MD08G1192900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003125
Physical Location & Seq
Reverse (-)
24846677 .. 24848559
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1192900.v1.1.491

Sequence Viewer

Length: 924 bp
ATGCCACCATATGCATCTTCCGAGTTGCCTCATCCACAAGTCGGCAGACCAAATGAACCTCACGGTTGGTTCTATTGCTTACCCTGTATCAGACAGGCTGTCATGCCTGCCACCGATAATGGTCTCAAAGAGAGAGTTTCTTCTGCCCGTCATGGAATTCCTGGGGAGACATTTGTGCCGAATGCAGACGGTGCCTTTGAACAGAAGAGACTCCTTGTTAACAATCCTTGTGGGGATCAGACAACTTTGATCTTTAGTTCTGGGATGGTGAATCCCATGCAGTGCCGTACTTCTTGGAATCCGTTCCAACAAGGAGCTTATTATTTGGATGGGGATGATCCTCGAAATGAAAGAGATTTTAAAAATTTATCGGGAGCAGCTTTGACTGATGAATTCAAAGGAAGTGATGAAAATGGTGCTGAAAGTGAGATGCATGAAGACACCGAAGAACTGAATGCCTTGCTCTACTCAGATGGTGATAGCGATTATACCGAGGACGATGAAGTTACTAGCACAGGCCATTCCCCTAGTACAATGACAGTTCATGACAAGCAAAATTGGTTTGAAGAAGTTGCTAGTTCTGATGGAATGGATAAAAAGCGGAAGCTATTTGATGAACGTTATGATCATGTACCATCTGTCATGGACACTGCGAGTTCCATGAAACACAACCGACCTCTGGAGTTGGAGGATGATGCGGAATCCAGTTGTGCCTGCAACAGAAGTTCTGGATTAAGAGAAGTGGATTCCTTTACCAGCAACAAGAAGATGAGAAAGGAAAAAATCCGTGAAACCATTAACATTCTGCAGAACATAATTCCCGATGGCAAGGGAAAGGATGCGATGGTGGTGCTGGATGAAGCAATCCACTACTTAAAGTCGTTGAAGCTCAAGGCCAAGGCTTTGGGACTCGACTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

34.29

Weight (kDa)

4.87

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 253 - 298 1.1e-14 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 191
AciI CCGC 2 cut(s) 601, 698
AclI AACGTT 1 cut(s) 619
AclWI GGATC 2 cut(s) 243, 332
AcsI RAATTY 3 cut(s) 156, 364, 392
AfaI GTAC 3 cut(s) 289, 532, 633
AfiI CCNNNNNNNGG 2 cut(s) 41, 679
AgsI TTSAA 4 cut(s) 200, 397, 566, 886
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 4 cut(s) 317, 380, 607, 889
AluI AGCT 4 cut(s) 317, 380, 607, 889
Alw26I GTCTC 3 cut(s) 128, 161, 202
AlwI GGATC 2 cut(s) 243, 332
AoxI GGCC 2 cut(s) 517, 894
ApeKI GCWGC 1 cut(s) 377
ApoI RAATTY 3 cut(s) 156, 364, 392
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
Asp700I GAANNNNTTC 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 280, 488
BanI GGYRCC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 389
BccI CCATC 7 cut(s) 259, 323, 467, 578, 643, 818, 838
BceAI ACGGC 1 cut(s) 270
BcgI CGANNNNNNTGC 2 cut(s) 832, 866
BciT130I CCWGG 1 cut(s) 162
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 3 cut(s) 128, 161, 202
BfaI CTAG 3 cut(s) 510, 528, 576
BfmI CTRYAG 1 cut(s) 806
BisI GCNGC 1 cut(s) 378
BlsI GCNGC 1 cut(s) 379
Bme1390I CCNGG 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 4 cut(s) 23, 420, 685, 829
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 701
BpuEI CTTGAG 1 cut(s) 875
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 161, 492, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 679
Bse1I ACTGG 1 cut(s) 705
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 161, 492, 897
BseGI GGATG 7 cut(s) 31, 270, 334, 340, 697, 844, 862
BseLI CCNNNNNNNGG 2 cut(s) 41, 679
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 705
BseXI GCAGC 1 cut(s) 389
BshFI GGCC 2 cut(s) 519, 896
BshNI GGYRCC 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 41, 679
BsmAI GTCTC 3 cut(s) 128, 161, 202
BsmI GAATGC 2 cut(s) 187, 460
BsnI GGCC 2 cut(s) 519, 896
Bso31I GGTCTC 1 cut(s) 128
Bsp143I GATC 4 cut(s) 235, 249, 337, 625
BspACI CCGC 2 cut(s) 601, 698
BspANI GGCC 2 cut(s) 519, 896
BspCNI CTCAG 1 cut(s) 482
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 810
BspPI GGATC 2 cut(s) 243, 332
BspT107I GGYRCC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 3 cut(s) 161, 492, 897
BssMI GATC 4 cut(s) 235, 249, 337, 625
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 65, 191, 541
Bst6I CTCTTC 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 108, 715
BstDEI CTNAG 1 cut(s) 469
BstF5I GGATG 7 cut(s) 31, 270, 334, 340, 697, 844, 862
BstKTI GATC 4 cut(s) 238, 252, 340, 628
BstMAI GTCTC 3 cut(s) 128, 161, 202
BstMBI GATC 4 cut(s) 235, 249, 337, 625
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 806
BstV1I GCAGC 1 cut(s) 389
BstV2I GAAGAC 1 cut(s) 444
BstXI CCANNNNNNTGG 1 cut(s) 904
BsuRI GGCC 2 cut(s) 519, 896
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 7 cut(s) 31, 270, 334, 340, 697, 844, 862
BtsI GCAGTG 2 cut(s) 287, 648
BtsIMutI CAGTG 2 cut(s) 287, 648
Cac8I GCNNGC 2 cut(s) 108, 715
CciI TCATGA 1 cut(s) 544
Csp6I GTAC 3 cut(s) 288, 531, 632
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 8 cut(s) 103, 152, 277, 434, 545, 629, 643, 661
CviJI RGCY 8 cut(s) 98, 317, 380, 519, 607, 889, 896, 902
CviKI_1 RGCY 8 cut(s) 98, 317, 380, 519, 607, 889, 896, 902
CviQI GTAC 3 cut(s) 288, 531, 632
DdeI CTNAG 1 cut(s) 469
DpnI GATC 4 cut(s) 237, 251, 339, 627
DpnII GATC 4 cut(s) 235, 249, 337, 625
DraI TTTAAA 1 cut(s) 361
DriI GACNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 200
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 897
Eco31I GGTCTC 1 cut(s) 128
EcoRI GAATTC 2 cut(s) 156, 392
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 897
EcoT22I ATGCAT 2 cut(s) 16, 435
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 8 cut(s) 106, 155, 280, 437, 548, 632, 646, 664
FatI CATG 8 cut(s) 102, 151, 276, 433, 544, 628, 642, 660
FauNDI CATATG 1 cut(s) 10
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 1 cut(s) 378
FokI GGATG 7 cut(s) 18, 277, 341, 347, 704, 851, 869
Fsp4HI GCNGC 1 cut(s) 378
FspBI CTAG 3 cut(s) 510, 528, 576
GluI GCNGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 2 cut(s) 519, 896
Hin1II CATG 8 cut(s) 106, 155, 280, 437, 548, 632, 646, 664
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 7 cut(s) 210, 271, 298, 701, 746, 909, 914
HpaI GTTAAC 1 cut(s) 220
HphI GGTGA 2 cut(s) 280, 488
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 6 cut(s) 22, 92, 240, 472, 583, 923
Hpy188III TCNNGA 5 cut(s) 372, 545, 680, 729, 821
Hpy8I GTNNAC 1 cut(s) 220
HpyCH4III ACNGT 3 cut(s) 65, 191, 541
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 6 cut(s) 14, 185, 280, 433, 717, 808
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 1 cut(s) 469
HpySE526I ACGT 1 cut(s) 619
Hsp92II CATG 8 cut(s) 106, 155, 280, 437, 548, 632, 646, 664
Ksp22I TGATCA 1 cut(s) 625
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 235, 249, 337, 625
LmnI GCTCC 2 cut(s) 314, 374
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 4 cut(s) 23, 420, 685, 829
MaeI CTAG 3 cut(s) 510, 528, 576
MaeII ACGT 1 cut(s) 619
MaeIII GTNAC 1 cut(s) 505
MalI GATC 4 cut(s) 237, 251, 339, 627
MboI GATC 4 cut(s) 235, 249, 337, 625
MboII GAAGA 7 cut(s) 9, 132, 217, 449, 458, 578, 778
MluCI AATT 5 cut(s) 156, 364, 392, 556, 816
MlyI GAGTC 3 cut(s) 204, 903, 908
MmeI TCCRAC 2 cut(s) 331, 666
MnlI CCTC 6 cut(s) 39, 69, 351, 487, 682, 687
Mph1103I ATGCAT 2 cut(s) 16, 435
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 5 cut(s) 219, 360, 734, 798, 875
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 2 cut(s) 187, 460
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeI CATATG 1 cut(s) 10
NdeII GATC 4 cut(s) 235, 249, 337, 625
NlaIII CATG 8 cut(s) 106, 155, 280, 437, 548, 632, 646, 664
NlaIV GGNNCC 1 cut(s) 193
NsiI ATGCAT 2 cut(s) 16, 435
PagI TCATGA 1 cut(s) 544
PctI GAATGC 2 cut(s) 187, 460
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 4 cut(s) 271, 298, 701, 746
PkrI GCNGC 1 cut(s) 379
PleI GAGTC 3 cut(s) 204, 903, 908
PpsI GAGTC 3 cut(s) 204, 903, 908
Psp1406I AACGTT 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 193
PstI CTGCAG 1 cut(s) 810
RsaI GTAC 3 cut(s) 289, 532, 633
RsaNI GTAC 3 cut(s) 288, 531, 632
SaqAI TTAA 5 cut(s) 219, 360, 734, 798, 875
SatI GCNGC 1 cut(s) 378
Sau3AI GATC 4 cut(s) 235, 249, 337, 625
SchI GAGTC 3 cut(s) 204, 903, 908
ScrFI CCNGG 1 cut(s) 162
SetI ASST 7 cut(s) 61, 319, 382, 609, 622, 679, 891
SfaNI GCATC 4 cut(s) 23, 420, 685, 829
SfcI CTRYAG 1 cut(s) 806
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
Sse9I AATT 5 cut(s) 156, 364, 392, 556, 816
SsiI CCGC 2 cut(s) 601, 698
SspMI CTAG 3 cut(s) 510, 528, 576
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 3 cut(s) 65, 191, 541
TaiI ACGT 1 cut(s) 622
TaqI TCGA 2 cut(s) 343, 912
TasI AATT 5 cut(s) 156, 364, 392, 556, 816
TatI WGTACW 1 cut(s) 530
TfiI GAWTC 4 cut(s) 271, 298, 701, 746
Tru1I TTAA 5 cut(s) 219, 360, 734, 798, 875
Tru9I TTAA 5 cut(s) 219, 360, 734, 798, 875
TscAI CASTG 2 cut(s) 287, 655
TseI GCWGC 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 291, 776
TspRI CASTG 2 cut(s) 287, 655
XapI RAATTY 3 cut(s) 156, 364, 392
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 3 cut(s) 510, 528, 576
Zsp2I ATGCAT 2 cut(s) 16, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.