MD15G1381700.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003125
Physical Location & Seq
Reverse (-)
47044622 .. 47046542
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1381700.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGGTGCTCCACATGGCTTGGGGCAACAAAACTTCATTACTGCTTATGGGAAACCAGGCACTAATATGCTTTTGACAAATGGTACTATGCCGCCATATGCATCCTCAGAGTTGCCGCAACCACAAGTCGGCAAACCAAATGAACCTAAAGGTTGGTTCTATTGCTTACCCCGTATCAGACAGGCCTTCATGCCTGCCACCGACAATGGTCTCAAAGAGATAGTTTCTTCTGCCTGTCATGAAAGTCATGGGGGGACATTCGTACCGTACGCAGACAGTGCCTTTGAACAGAAGAGACTCCTTGTTAACAATCTTTCAGGGTATCGGACAACTTTGGTCTTTAGTTCTGAGATAGTGAATCCGTTGCAGTGCCGTACTTCTTGGAATCCAGACCAACAAGGTGCTTATTATTTGGATGGGGATGATCCTCGAAATGAAAGAGATTTTAAAAATCTGTCGGGAGCAGTTTTGACTGATGAATTCAGAGGAAATGATGAATGTGGTGCTGAAAGTGAGATGCATGAAGACACAGAAGAACTGAATGCCTTGCTCTATTCAGATGGTGATAGCGATTATACTGAGGATGATGAAGTTACTAGCACAGGCCATTCTCCGAGTACAACGACAGTTCATGATAAGCAAAATTGGTTTGAAGAAGTTGCCAGTTCTGATGGAATGAATAAAAAGCGGAAGCTATTTGATGGTGGTAACGATGGTGTGCCATCCGTAATGGACACTGCTAGTCCCATGAAACACAACCGATCGCTGGAGTTAGAGGATGATGCAGAATCCAGTTGTGCCTGCCAGCGAAGTTCTGGATTAAGAGAAGTGGATTCCTTGTCCAGCAACAAGAAGATGAGAAAGGAGAAAATCCGCGAAACCATTAACATTCTGCAGAACATAATTCCCGATGGAAAGGGAAAGGATGCGATTGTGGTGCTCGATGAAGCAATCCACTACTTGAAATCGTTGAAGCTCAAAGCCAAGGCTTTCGGACTTGACTCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

37.1

Weight (kDa)

4.92

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 282 - 327 1.7e-14 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 876
AciI CCGC 4 cut(s) 92, 116, 688, 874
AclWI GGATC 1 cut(s) 419
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 5 cut(s) 85, 264, 269, 376, 619
AfiI CCNNNNNNNGG 2 cut(s) 128, 766
AgsI TTSAA 4 cut(s) 287, 653, 964, 973
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 2 cut(s) 694, 976
AluI AGCT 2 cut(s) 694, 976
Alw21I GWGCWC 2 cut(s) 10, 942
Alw26I GTCTC 2 cut(s) 215, 289
AlwI GGATC 1 cut(s) 419
AoxI GGCC 2 cut(s) 183, 604
ApoI RAATTY 1 cut(s) 479
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AsuHPI GGTGA 1 cut(s) 575
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 10, 942
BccI CCATC 7 cut(s) 410, 554, 665, 695, 707, 730, 905
BceAI ACGGC 1 cut(s) 357
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 215, 289
BfaI CTAG 2 cut(s) 597, 741
BfmI CTRYAG 1 cut(s) 893
BisI GCNGC 2 cut(s) 92, 116
BlsI GCNGC 2 cut(s) 93, 117
Bme1390I CCNGG 1 cut(s) 57
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 4 cut(s) 110, 507, 772, 916
BoxI GACNNNNGTC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 984
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 766
Bse1I ACTGG 2 cut(s) 663, 792
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 984
BseGI GGATG 7 cut(s) 101, 421, 427, 589, 722, 784, 931
BseLI CCNNNNNNNGG 2 cut(s) 128, 766
BseMII CTCAG 3 cut(s) 120, 339, 570
BseNI ACTGG 2 cut(s) 663, 792
Bsh1236I CGCG 1 cut(s) 876
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 2 cut(s) 185, 606
BsiEI CGRYCG 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 10, 942
BsiWI CGTACG 1 cut(s) 267
BslFI GGGAC 2 cut(s) 268, 729
BslI CCNNNNNNNGG 2 cut(s) 128, 766
BsmAI GTCTC 2 cut(s) 215, 289
BsmFI GGGAC 2 cut(s) 268, 729
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 2 cut(s) 185, 606
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 10, 942
Bsp143I GATC 2 cut(s) 424, 761
BspACI CCGC 4 cut(s) 92, 116, 688, 874
BspANI GGCC 2 cut(s) 185, 606
BspCNI CTCAG 3 cut(s) 119, 340, 571
BspFNI CGCG 1 cut(s) 876
BspHI TCATGA 2 cut(s) 238, 631
BspMAI CTGCAG 1 cut(s) 897
BspPI GGATC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 215
BsrI ACTGG 2 cut(s) 663, 792
BssECI CCNNGG 1 cut(s) 984
BssMI GATC 2 cut(s) 424, 761
BssT1I CCWWGG 1 cut(s) 984
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 267, 278, 628
Bst6I CTCTTC 1 cut(s) 287
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 195, 802, 806
BstDEI CTNAG 3 cut(s) 106, 348, 579
BstF5I GGATG 7 cut(s) 101, 421, 427, 589, 722, 784, 931
BstFNI CGCG 1 cut(s) 876
BstKTI GATC 2 cut(s) 427, 764
BstMAI GTCTC 2 cut(s) 215, 289
BstMBI GATC 2 cut(s) 424, 761
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 1 cut(s) 57
BstPAI GACNNNNGTC 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 893
BstUI CGCG 1 cut(s) 876
BstV2I GAAGAC 1 cut(s) 531
BsuRI GGCC 2 cut(s) 185, 606
BtsCI GGATG 7 cut(s) 101, 421, 427, 589, 722, 784, 931
BtsI GCAGTG 2 cut(s) 374, 735
BtsIMutI CAGTG 3 cut(s) 283, 374, 735
Cac8I GCNNGC 3 cut(s) 195, 802, 806
CciI TCATGA 2 cut(s) 238, 631
Csp6I GTAC 5 cut(s) 84, 263, 268, 375, 618
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 7 cut(s) 14, 190, 239, 248, 521, 632, 748
CviJI RGCY 7 cut(s) 18, 185, 606, 694, 976, 983, 989
CviKI_1 RGCY 7 cut(s) 18, 185, 606, 694, 976, 983, 989
CviQI GTAC 5 cut(s) 84, 263, 268, 375, 618
DdeI CTNAG 3 cut(s) 106, 348, 579
DpnI GATC 2 cut(s) 426, 763
DpnII GATC 2 cut(s) 424, 761
DraI TTTAAA 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 984
Eco147I AGGCCT 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 2 cut(s) 103, 522
ErhI CCWWGG 1 cut(s) 984
FaeI CATG 7 cut(s) 17, 193, 242, 251, 524, 635, 751
FaqI GGGAC 2 cut(s) 268, 729
FatI CATG 7 cut(s) 13, 189, 238, 247, 520, 631, 747
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 92, 116
FokI GGATG 7 cut(s) 88, 428, 434, 596, 709, 791, 938
Fsp4HI GCNGC 2 cut(s) 92, 116
FspBI CTAG 2 cut(s) 597, 741
GluI GCNGC 2 cut(s) 92, 116
GsuI CTGGAG 1 cut(s) 788
HaeIII GGCC 2 cut(s) 185, 606
Hin1II CATG 7 cut(s) 17, 193, 242, 251, 524, 635, 751
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 6 cut(s) 297, 358, 385, 788, 833, 1001
HpaI GTTAAC 1 cut(s) 307
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 1 cut(s) 307
Hpy188III TCNNGA 6 cut(s) 239, 389, 459, 632, 816, 908
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 3 cut(s) 267, 278, 628
HpyCH4V TGCA 5 cut(s) 101, 367, 520, 785, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 3 cut(s) 106, 348, 579
Hsp92II CATG 7 cut(s) 17, 193, 242, 251, 524, 635, 751
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 424, 761
LmnI GCTCC 2 cut(s) 13, 461
LweI GCATC 4 cut(s) 110, 507, 772, 916
MaeI CTAG 2 cut(s) 597, 741
MaeIII GTNAC 2 cut(s) 592, 707
MalI GATC 2 cut(s) 426, 763
MboI GATC 2 cut(s) 424, 761
MboII GAAGA 6 cut(s) 219, 304, 536, 545, 665, 865
MhlI GDGCHC 2 cut(s) 10, 942
MluCI AATT 3 cut(s) 479, 643, 903
MlyI GAGTC 2 cut(s) 291, 995
MnlI CCTC 5 cut(s) 115, 438, 479, 574, 769
Mph1103I ATGCAT 2 cut(s) 103, 522
MseI TTAA 4 cut(s) 306, 447, 821, 885
MslI CAYNNNNRTG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 57
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeI CATATG 1 cut(s) 97
NdeII GATC 2 cut(s) 424, 761
NlaIII CATG 7 cut(s) 17, 193, 242, 251, 524, 635, 751
NsiI ATGCAT 2 cut(s) 103, 522
PagI TCATGA 2 cut(s) 238, 631
PceI AGGCCT 1 cut(s) 185
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 4 cut(s) 358, 385, 788, 833
Pfl23II CGTACG 1 cut(s) 267
PkrI GCNGC 2 cut(s) 93, 117
Ple19I CGATCG 1 cut(s) 764
PleI GAGTC 2 cut(s) 291, 995
PpsI GAGTC 2 cut(s) 291, 995
PshAI GACNNNNGTC 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspLI CGTACG 1 cut(s) 267
PstI CTGCAG 1 cut(s) 897
PvuI CGATCG 1 cut(s) 764
RsaI GTAC 5 cut(s) 85, 264, 269, 376, 619
RsaNI GTAC 5 cut(s) 84, 263, 268, 375, 618
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 4 cut(s) 306, 447, 821, 885
SatI GCNGC 2 cut(s) 92, 116
Sau3AI GATC 2 cut(s) 424, 761
SchI GAGTC 2 cut(s) 291, 995
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 2 cut(s) 10, 942
SetI ASST 5 cut(s) 148, 154, 403, 696, 978
SfaNI GCATC 4 cut(s) 110, 507, 772, 916
SfcI CTRYAG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 3 cut(s) 479, 643, 903
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 4 cut(s) 92, 116, 688, 874
SspMI CTAG 2 cut(s) 597, 741
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 3 cut(s) 267, 278, 628
TaqI TCGA 2 cut(s) 430, 942
TasI AATT 3 cut(s) 479, 643, 903
TatI WGTACW 1 cut(s) 617
TauI GCSGC 2 cut(s) 94, 118
TfiI GAWTC 4 cut(s) 358, 385, 788, 833
Tru1I TTAA 4 cut(s) 306, 447, 821, 885
Tru9I TTAA 4 cut(s) 306, 447, 821, 885
TscAI CASTG 3 cut(s) 283, 374, 742
TspGWI ACGGA 2 cut(s) 351, 715
TspRI CASTG 3 cut(s) 283, 374, 742
XapI RAATTY 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 812
XspI CTAG 2 cut(s) 597, 741
Zsp2I ATGCAT 2 cut(s) 103, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.