Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001895

Physical Location & Seq

Genomic Coordinates Forward (+)

25657145 .. 25666361

Gene Structure 9217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1198000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3831 bp

ATGGATAGTTCGGAATGTGTTCGAGTGGCTGTCAATGTAAGGCCCTTGATTACCTCCGAGCTTCTGGTAGGCTGCACAGATTGCATTTCCGTCGTTCCCGGTGAACCCCAGGTGCAAATTGGGACACATTCGTTCACATACGATTATGTGTATGGTAGCACCGGACTTCCTTCTAATGCGGTGTATGATGATTGTGTTGCTCCGCTCGTTGATGCCCTTTTCCATGGATACAATGCTACTGTACTTGCTTATGGACAGACGGGTTCCGGGAAGACTTATACAATGGGGACTAACTATACTGGAGAAGGAAGTAATGGTGGAATAATACCCAAGGTGATGGAAAGTATATTCAAGAAAGTTGAGACAACAAAAGATAACACAGAGTTTTTGATCAGGGTATCATTTATTGAGATATTTAAGGAGGAAGTGTTTGATTTGCTTGACCCAAATTCATCATCACTGCCCAAAAATGAAGGGGCAGCACCTGCAAAGCCTGCGCCGGCAAGAGTGCCTATCCAAATTAGAGAAACAGTGAATGGAGGGATAACTCTTGCTGGTGTCACTGAGGCAGAGGTTAGAACAAAAGAAGAGATGGCGTCATATCTCACTCGTGGATCATTATCTCGTGCTACTGGGAGCACCAATATGAATAGCCAGTCAAGCCGTTCACATGCAATATTTACAATAACAATGGAGCAAAAGAGGATTTCTCACTCTGTGAATGGAGCAAATAGTGACGATATTGGTGATGATATCCTTTGCGCCAAGCTCCATTTAGTGGATTTAGCAGGGTCTGAGCGGGCAAAACGGACAGGTGCTGACGGAATGAGGTTGAAAGAAGGCATTCATATTAACAAGGGTTTACTTGCTCTTGGTAATGTGATAAGTGCCTTGGGAGATGAAAAGAAGCGGAAAGAAGGCGGCCATGTTCCGTATCGTGATAGCAAATTAACACGTTTATTACAGGATTCGCTCGGAGGAAACAGTAAGACTGTGATGATTGCTTGTGTTAGTCCAGCTGACACAAATGCTGAAGAGACGTTAAATACTTTGAAATATGCTAACCGTGCTCGCAACATCCACAATAAGGCAGTTGTCAATCGTGATCCCATGGCAGCTCAGTTGCAAACAATGAGAAGCCAGATTGAACAGTTACAGACTGAACTTTTATTTTATCGTGGTGATGCCAGTGCACCATATGAGGAACTTCAGATCCTGAAGCACAAAGTATCATTACTCGAAGCAAGCAACGTGGAGTTGAGGCACGAGCTTCAAGAACGTCGAGTTTCATGCGAACACCTAAAGCAACGTGCTCTTGATGCTCAGGTTGAAAAAGATAAACTGGCCATGATAATTGAATCAGCACGAAGTGGTAAATCTTGGGATGAGATTGACTCAAATTCAGTTCAGGATTATGACTTGCTAAAAGGTTACGTGTCAAAAATTCAGGAGTTAGAAGGGGAGTTATTGTGCTTGAAAAACTCAAACAACTCAAAACACAATAGATTTGTTGACTGTGCTGATGATGGCGGCTTTCGTTCTAAGAATATTTTATTCCCCTCTATCAATGACTATTCATCTGATTATGATACTAAAGCTGAGGATATTTCAGATGAGATTGAAGATCATGAAAAGGAGCAAAAGGAGCAAGAATTTTCTTCTCTTCAAGAAAAATTTGATAGGGAGCTCAAAGAATTGGACAAAGCTCTTGAACAGAAGGAGGCTGAGATGAAGCGGTTTACTACTTCTGATACCTCGGTTCTTAAACTACATTATGAGAAGAAAGTTCAGGAGTTAGAAATTGAAAAGAAAACATTGCAGAAAGAGATTGAGGCGTTGAAACACAATCTTTCCAATATATCATCTACTTCTGATGATGGTGCTCAAAAGTTGAAGGAAGATTATCTACACAAGTTGAATGTCCTTGAAGGGCAGGTTTCTGAGCTAAAAAAGAAGCAAGATGCTCAAGCTCAACTATTGAGACAAAAACAAAAAAGTGATGAGGCTGCAAAGCGACTTCAGGATGAGATACAGAGAATAAAGACTCAGAAGGTTCAACTACAACACAAGATTAAACAAGAGTCTGAGCAGTTCAGATTATGGAAAGCATCACGAGAAAAAGAAGTTCTTCAGCTTAAGAAAGAGGGGAGGAGGAATGAGTATGAAATGCATAAACTATTAGCTTTGAATCAGAGGCAAAAAATGGTTTTGCAACGGAAGACAGAAGAAGCTTCTATGGCCACAAAAAGGCTAAAAGAGCTTTTAGAATCGCGAAAGACTTCTTCACGTGAAATTTCTGGAGCTGGCAGTGGACATGGTCCTGCAGTTCAGGCCTTGATGCAGGCAATTGAACATGAGCTTGAAGTCACAGTTCGGGTGCATGAAGTACGATCTGAGTATGACCGTCAAATGGAAGAGCGGGCTAGGATGGCCAAGGAGGTCGCAAAGCTAAAAGAAGAAGCAGAAATGTCAAAACGAACTAATTTCAGTGATTACCCCGAATCAATGTCTCCTGGTGCGAGAAATTCAAGGATTTATGCACTTGAAAACATGCTTGCTACTTCATCTAGCACCCTGGTTTCCATGGCATCACAATTGTCAGAAGCAGAAGAGCGCGAGCGGGGTTTTACTGGGAGGGGACGCTGGAACCATGTTCGGTCTCTTGCTGATGCAAAAAATTTGATGAATCATTTGTTCAATTTAGCTTCTTCCTCCAGGTGTTTGTTACGAGATAAAGAAGTTGCATACAGAGAGAGGGATTTGGAAATAAGAGATTTGAAGGAAAAAGTTGTAAGCCTGAGTGGTTTGCTTAGAAAATCAGAGATGCAGAGGGCTGAACTTGTTCATCAGAATCAGGCGTTGAAGAAATTTGCCATGAACTGTTCTAAGGATGCGGACCTAAACAATGGTGGACACAAGTATGATTTGCGCAAGTTGGAAAACCGGGCGTCATTTATCTGGGAGGATATGGATACGTCTGATTCAGAAAAATCTGATGCGGAGGAGGATGGCGACTGGGTAATGTCAAGAAAACGACCATCTAAAAAGCGAAAATCCAAAAGTGGAAGTTCAAGTGGGGGAGGATATAATGAGTCAGAAGTTAAGAATTTTGGAGGCTTCAAATTAGACGCTTCAGGTGATGGGATTGTGTCTCTGAAGAAAAGTGAATCAGGCGTATGCTGCTCTTGCAGCAAAAGCTCATCATGCAAAACTAGCAAATGTGAATGCCGGTCTTCCGCAGGCACTTGTGGGTTATCATGTGGCTGTGCGGCTACAAAGTGTTCAAATAGAGAATCAGCTTCACTTGTGGTGCAAGAATCTGCACAAGCGGAGGTGTCTGAAGGGATTAGAGATGAGATCGGGACTGATGAAGCAGAGAAGAATCAGCTTCTTGTTACTCATGGTGCAAGGTTGCTTCAGAATGCATTAGCTGATAACTCTAGTGAGACAACTGATGATGGTGAACCAAGAAGGAAGGCTCTTTCCGAAATTGGAAATACGTTGGTCAAATCCAACGCACCAAAGCCTAACCAGAGAAAGAAGTGGCGAAAATCCACAATTCAACTTGTTACAAATGCTCCGCCTCCCTCGCAGCCAGAAGTTGCTGAAGCACCTCGAAGGCCAGATAACAAAGGACAAGAGGCAAGCATCCCTATGAAGCTACCAAGGGCCATGCGATCAGCTGCTGCATCAAACAGCGGCAACCCATTTAGAGACAGGAATGCCGATAAACCACAGCAATCGGATGCCAACAAGGAAGCTGGAATTCCCGCACCAAGAAGTCCTCTCCGGCAGAAGAGAACAAAAGATGAGAAGGAAAACCACCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0008574 GO:0009506 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0055044 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1277

Amino Acids

142.32

Weight (kDa)

7.71

Isoelectric Point (pI)

50.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	6 - 147	4.9e-19	Microtubule binding
Kinesin	PF00225	38 - 360	6.5e-106	Kinesin motor domain
KIF21A_4th	PF25764	537 - 715	6.5e-55	KIF21A-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60930 AT5G60930 AT5G60930
fragaria_vesca	FvH4_5g35340
malus_domestica	MD08G1198000.v1.1 MD15G1385300.v1.1
prunus_persica	Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1
pyrus_communis	pycom08g17020 pycom15g07240 pycom15g34540
rosa_chinensis	RchiOBHm_Chr3g0494071 RchiOBHm_Chr7g0236541 RchiOBHm_Chr7g0236581 RchiOBHm_Chr7g0236691
rosa_laevigata	RLG00000000987 RLG00000000988 RLG00000001068 RLG00000001077 RLG00000001104
rosa_multiflora	Rmu_sc0002536.1_g000016 Rmu_sc0003720.1_g000010 Rmu_sc0011298.1_g000005 Rmu_ssc0000050.1_g000117
rosa_roxburghii	Rroxscaffold_3G00224960 Rroxscaffold_6G00429280
rosa_rugosa	Rorug07G0291900 Rorug07G0291900 Rorug07G0297600 Rorug07G0297700 Rorug07G0298200
rosa_samantha	Rh2CG391900 Rh7AG453300 Rh7AG453600 Rh7BG401100 Rh7BG418700 Rh7BG424500 Rh7CG471900 Rh7CG472100 Rh7DG438700 Rh7DG440900 Rh7DG441000
rosa_wichuraiana	Rw0G020690 Rw7G037620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	493
Acc16I	TGCGCA	1 cut(s)	2930
Acc36I	ACCTGC	2 cut(s)	493, 1924
AccB7I	CCANNNNNTGG	1 cut(s)	778
AccBSI	CCGCTC	4 cut(s)	205, 799, 2419, 2620
AccII	CGCG	2 cut(s)	2272, 2616
AclWI	GGATC	3 cut(s)	622, 1100, 1207
AcoI	YGGCCR	4 cut(s)	922, 1344, 2238, 2430
AcvI	CACGTG	1 cut(s)	2288
AcyI	GRCGYC	2 cut(s)	596, 2948
AdeI	CACNNNGTG	2 cut(s)	718, 1370
AfaI	GTAC	2 cut(s)	243, 2388
AfiI	CCNNNNNNNGG	4 cut(s)	778, 1087, 2247, 2410
AflII	CTTAAG	1 cut(s)	2135
AflIII	ACRYGT	2 cut(s)	953, 1434
AjnI	CCWGG	4 cut(s)	108, 2512, 2574, 2714
AloI	GAACNNNNNNTCC	2 cut(s)	1197, 1229
Alw21I	GWGCWC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
Alw26I	GTCTC	8 cut(s)	356, 1031, 1975, 2514, 2664, 3156, 3440, 3708
Alw44I	GTGCAC	1 cut(s)	1191
AlwI	GGATC	3 cut(s)	622, 1100, 1207
AlwNI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3686
AoxI	GGCC	8 cut(s)	41, 922, 1344, 2238, 2331, 2430, 3620, 3669
ApaLI	GTGCAC	1 cut(s)	1191
ApeKI	GCWGC	9 cut(s)	72, 479, 1115, 2006, 3180, 3189, 3592, 3683, 3686
Asp700I	GAANNNNTTC	5 cut(s)	18, 843, 2127, 2278, 2841
AspLEI	GCGC	4 cut(s)	499, 764, 2616, 2931
AspS9I	GGNCC	4 cut(s)	42, 2318, 2896, 3669
AsuC2I	CCSGG	3 cut(s)	99, 268, 2945
AsuHPI	GGTGA	6 cut(s)	113, 346, 758, 1193, 3149, 3473
AvaII	GGWCC	2 cut(s)	2318, 2896
BaeGI	GKGCMC	1 cut(s)	1195
BaeI	ACNNNNGTAYC	2 cut(s)	1743, 1776
BalI	TGGCCA	3 cut(s)	1346, 2240, 2432
BanII	GRGCYC	1 cut(s)	1689
BarI	GAAGNNNNNNTAC	2 cut(s)	1890, 1922
BauI	CACGAG	4 cut(s)	609, 624, 1265, 2112
BbrPI	CACGTG	1 cut(s)	2288
BbsI	GAAGAC	3 cut(s)	278, 2225, 3225
Bbv12I	GWGCWC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
BbvCI	CCTCAGC	1 cut(s)	1599
BbvI	GCAGC	9 cut(s)	59, 491, 1127, 1993, 3167, 3201, 3604, 3670, 3673
BccI	CCATC	9 cut(s)	331, 586, 1520, 1871, 2422, 3002, 3046, 3134, 3452
BceAI	ACGGC	1 cut(s)	648
BcgI	CGANNNNNNTGC	4 cut(s)	73, 107, 1272, 1306
BciT130I	CCWGG	4 cut(s)	110, 2514, 2576, 2716
BciVI	GTATCC	2 cut(s)	221, 2965
BclI	TGATCA	1 cut(s)	390
BcnI	CCSGG	3 cut(s)	99, 268, 2945
BcoDI	GTCTC	8 cut(s)	356, 1031, 1975, 2514, 2664, 3156, 3440, 3708
BfaI	CTAG	4 cut(s)	2424, 2568, 3213, 3441
BfmI	CTRYAG	1 cut(s)	2322
BfrI	CTTAAG	1 cut(s)	2135
BfuAI	ACCTGC	2 cut(s)	493, 1924
BfuI	GTATCC	2 cut(s)	221, 2965
Bme1390I	CCNGG	7 cut(s)	99, 110, 268, 2514, 2576, 2716, 2945
Bme18I	GGWCC	2 cut(s)	2318, 2896
BmgT120I	GGNCC	4 cut(s)	42, 2318, 2896, 3669
BmiI	GGNNCC	2 cut(s)	265, 2648
BmrFI	CCNGG	7 cut(s)	99, 110, 268, 2514, 2576, 2716, 2945
BmrI	ACTGGG	3 cut(s)	642, 2640, 3025
BmuI	ACTGGG	3 cut(s)	642, 2640, 3025
BpiI	GAAGAC	3 cut(s)	278, 2225, 3225
BplI	GAGNNNNNCTC	4 cut(s)	694, 726, 1379, 1411
BpmI	CTGGAG	3 cut(s)	321, 2319, 2698
Bpu10I	CCTNAGC	2 cut(s)	1323, 1599
BpuEI	CTTGAG	1 cut(s)	1950
BpuMI	CCSGG	3 cut(s)	99, 268, 2945
BsaAI	YACGTR	2 cut(s)	1435, 2288
BsaBI	GATNNNNATC	1 cut(s)	619
BsaHI	GRCGYC	2 cut(s)	596, 2948
BsaI	GGTCTC	1 cut(s)	2664
BsaWI	WCCGGW	1 cut(s)	161
BsaXI	ACNNNNNCTCC	6 cut(s)	413, 443, 1454, 1484, 3562, 3592
Bsc4I	CCNNNNNNNGG	4 cut(s)	778, 1087, 2247, 2410
Bse118I	RCCGGY	2 cut(s)	499, 3228
Bse1I	ACTGG	7 cut(s)	304, 637, 655, 1188, 1347, 2635, 3020
Bse3DI	GCAATG	1 cut(s)	1814
Bse8I	GATNNNNATC	1 cut(s)	619
BseBI	CCWGG	4 cut(s)	110, 2514, 2576, 2716
BseGI	GGATG	8 cut(s)	1077, 1390, 2029, 2433, 2896, 3013, 3648, 3751
BseJI	GATNNNNATC	1 cut(s)	619
BseLI	CCNNNNNNNGG	4 cut(s)	778, 1087, 2247, 2410
BseMI	GCAATG	1 cut(s)	1814
BseNI	ACTGG	7 cut(s)	304, 637, 655, 1188, 1347, 2635, 3020
BseRI	GAGGAG	2 cut(s)	2164, 3017
BseSI	GKGCMC	1 cut(s)	1195
BseXI	GCAGC	9 cut(s)	59, 491, 1127, 1993, 3167, 3201, 3604, 3670, 3673
BsgI	GTGCAG	2 cut(s)	58, 3306
Bsh1236I	CGCG	2 cut(s)	2272, 2616
BshFI	GGCC	8 cut(s)	43, 924, 1346, 2240, 2333, 2432, 3622, 3671
BsiHKAI	GWGCWC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
BsiSI	CCGG	7 cut(s)	99, 162, 267, 500, 2944, 3229, 3790
BslFI	GGGAC	4 cut(s)	136, 301, 2652, 3376
BslI	CCNNNNNNNGG	4 cut(s)	778, 1087, 2247, 2410
BsmAI	GTCTC	8 cut(s)	356, 1031, 1975, 2514, 2664, 3156, 3440, 3708
BsmBI	CGTCTC	1 cut(s)	1031
BsmFI	GGGAC	4 cut(s)	136, 301, 2652, 3376
BsmI	GAATGC	4 cut(s)	843, 3230, 3427, 3727
BsnI	GGCC	8 cut(s)	43, 924, 1346, 2240, 2333, 2432, 3622, 3671
Bso31I	GGTCTC	1 cut(s)	2664
Bsp1286I	GDGCHC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
Bsp143I	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
Bsp19I	CCATGG	3 cut(s)	223, 1110, 2583
Bsp68I	TCGCGA	1 cut(s)	2272
BspANI	GGCC	8 cut(s)	43, 924, 1346, 2240, 2333, 2432, 3622, 3671
BspFNI	CGCG	2 cut(s)	2272, 2616
BspHI	TCATGA	1 cut(s)	1627
BspLI	GGNNCC	2 cut(s)	265, 2648
BspMAI	CTGCAG	1 cut(s)	2326
BspMI	ACCTGC	2 cut(s)	493, 1924
BspPI	GGATC	3 cut(s)	622, 1100, 1207
BspQI	GCTCTTC	2 cut(s)	2409, 2604
BspTI	CTTAAG	1 cut(s)	2135
BspTNI	GGTCTC	1 cut(s)	2664
BsrBI	CCGCTC	4 cut(s)	205, 799, 2419, 2620
BsrDI	GCAATG	1 cut(s)	1814
BsrFI	RCCGGY	2 cut(s)	499, 3228
BsrI	ACTGG	7 cut(s)	304, 637, 655, 1188, 1347, 2635, 3020
BssAI	RCCGGY	2 cut(s)	499, 3228
BssMI	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
BssNI	GRCGYC	2 cut(s)	596, 2948
BssSI	CACGAG	4 cut(s)	609, 624, 1265, 2112
BssT1I	CCWWGG	7 cut(s)	223, 330, 891, 1110, 2433, 2583, 3665
Bst2BI	CACGAG	4 cut(s)	609, 624, 1265, 2112
Bst2UI	CCWGG	4 cut(s)	110, 2514, 2576, 2716
Bst6I	CTCTTC	6 cut(s)	582, 1029, 1668, 2409, 2604, 3791
BstACI	GRCGYC	2 cut(s)	596, 2948
BstAFI	CTTAAG	1 cut(s)	2135
BstAPI	GCANNNNNTGC	3 cut(s)	81, 485, 494
BstBAI	YACGTR	2 cut(s)	1435, 2288
BstDSI	CCRYGG	3 cut(s)	223, 1110, 2583
BstF5I	GGATG	8 cut(s)	1077, 1390, 2029, 2433, 2896, 3013, 3648, 3751
BstFNI	CGCG	2 cut(s)	2272, 2616
BstHHI	GCGC	4 cut(s)	499, 764, 2616, 2931
BstKTI	GATC	8 cut(s)	393, 617, 1108, 1215, 1627, 2393, 3360, 3680
BstMAI	GTCTC	8 cut(s)	356, 1031, 1975, 2514, 2664, 3156, 3440, 3708
BstMBI	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
BstNI	CCWGG	4 cut(s)	110, 2514, 2576, 2716
BstNSI	RCATGY	2 cut(s)	674, 2554
BstSCI	CCNGG	7 cut(s)	97, 108, 266, 2512, 2574, 2714, 2943
BstSFI	CTRYAG	1 cut(s)	2322
BstSLI	GKGCMC	1 cut(s)	1195
BstUI	CGCG	2 cut(s)	2272, 2616
BstV1I	GCAGC	9 cut(s)	59, 491, 1127, 1993, 3167, 3201, 3604, 3670, 3673
BstV2I	GAAGAC	3 cut(s)	278, 2225, 3225
BstX2I	RGATCY	1 cut(s)	1212
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	1 cut(s)	1212
BsuI	GTATCC	2 cut(s)	221, 2965
BsuRI	GGCC	8 cut(s)	43, 924, 1346, 2240, 2333, 2432, 3622, 3671
BtgI	CCRYGG	3 cut(s)	223, 1110, 2583
BtsCI	GGATG	8 cut(s)	1077, 1390, 2029, 2433, 2896, 3013, 3648, 3751
BtsI	GCAGTG	2 cut(s)	458, 2314
BtsIMutI	CAGTG	6 cut(s)	458, 537, 561, 1195, 2314, 2494
BtuMI	TCGCGA	1 cut(s)	2272
BveI	ACCTGC	2 cut(s)	493, 1924
CaiI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3686
CciI	TCATGA	1 cut(s)	1627
CfoI	GCGC	4 cut(s)	499, 764, 2616, 2931
Cfr10I	RCCGGY	2 cut(s)	499, 3228
Cfr13I	GGNCC	4 cut(s)	42, 2318, 2896, 3669
CseI	GACGC	4 cut(s)	585, 2649, 2937, 3137
Csp6I	GTAC	2 cut(s)	242, 2387
CspCI	CAANNNNNGTGG	2 cut(s)	1233, 1268
CviQI	GTAC	2 cut(s)	242, 2387
DpnI	GATC	8 cut(s)	392, 616, 1107, 1214, 1626, 2392, 3359, 3679
DpnII	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
DraIII	CACNNNGTG	2 cut(s)	718, 1370
EaeI	YGGCCR	4 cut(s)	922, 1344, 2238, 2430
Eam1104I	CTCTTC	6 cut(s)	582, 1029, 1668, 2409, 2604, 3791
EarI	CTCTTC	6 cut(s)	582, 1029, 1668, 2409, 2604, 3791
EciI	GGCGGA	1 cut(s)	3570
Ecl136II	GAGCTC	1 cut(s)	1687
Eco130I	CCWWGG	7 cut(s)	223, 330, 891, 1110, 2433, 2583, 3665
Eco147I	AGGCCT	1 cut(s)	2333
Eco24I	GRGCYC	1 cut(s)	1689
Eco31I	GGTCTC	1 cut(s)	2664
Eco32I	GATATC	1 cut(s)	754
Eco47I	GGWCC	2 cut(s)	2318, 2896
Eco53kI	GAGCTC	1 cut(s)	1687
Eco72I	CACGTG	1 cut(s)	2288
EcoICRI	GAGCTC	1 cut(s)	1687
EcoO109I	RGGNCCY	1 cut(s)	42
EcoRI	GAATTC	1 cut(s)	3765
EcoRII	CCWGG	4 cut(s)	108, 2512, 2574, 2714
EcoRV	GATATC	1 cut(s)	754
EcoT14I	CCWWGG	7 cut(s)	223, 330, 891, 1110, 2433, 2583, 3665
EcoT22I	ATGCAT	2 cut(s)	2172, 3427
EcoT38I	GRGCYC	1 cut(s)	1689
ErhI	CCWWGG	7 cut(s)	223, 330, 891, 1110, 2433, 2583, 3665
Esp3I	CGTCTC	1 cut(s)	1031
FalI	AAGNNNNNCTT	2 cut(s)	2112, 2144
FaqI	GGGAC	4 cut(s)	136, 301, 2652, 3376
FauI	CCCGC	4 cut(s)	792, 2412, 2613, 3778
FauNDI	CATATG	1 cut(s)	1198
FbaI	TGATCA	1 cut(s)	390
FokI	GGATG	8 cut(s)	1064, 1397, 2036, 2440, 2903, 3020, 3635, 3758
FriOI	GRGCYC	1 cut(s)	1689
FspBI	CTAG	4 cut(s)	2424, 2568, 3213, 3441
FspI	TGCGCA	1 cut(s)	2930
GlaI	GCGC	4 cut(s)	498, 763, 2615, 2930
GsuI	CTGGAG	3 cut(s)	321, 2319, 2698
HaeIII	GGCC	8 cut(s)	43, 924, 1346, 2240, 2333, 2432, 3622, 3671
HapII	CCGG	7 cut(s)	99, 162, 267, 500, 2944, 3229, 3790
HgaI	GACGC	4 cut(s)	585, 2649, 2937, 3137
HhaI	GCGC	4 cut(s)	499, 764, 2616, 2931
Hin1I	GRCGYC	2 cut(s)	596, 2948
Hin6I	GCGC	4 cut(s)	497, 762, 2614, 2929
HinP1I	GCGC	4 cut(s)	497, 762, 2614, 2929
HincII	GTYRAC	1 cut(s)	1513
HindII	GTYRAC	1 cut(s)	1513
HindIII	AAGCTT	1 cut(s)	2229
HpaII	CCGG	7 cut(s)	99, 162, 267, 500, 2944, 3229, 3790
HphI	GGTGA	6 cut(s)	113, 346, 758, 1193, 3149, 3473
Hpy99I	CGWCG	2 cut(s)	95, 1284
HpyCH4IV	ACGT	9 cut(s)	955, 1040, 1251, 1279, 1309, 1434, 2287, 2975, 3500
HpySE526I	ACGT	9 cut(s)	955, 1040, 1251, 1279, 1309, 1434, 2287, 2975, 3500
Hsp92I	GRCGYC	2 cut(s)	596, 2948
HspAI	GCGC	4 cut(s)	497, 762, 2614, 2929
KroI	GCCGGC	1 cut(s)	499
KroNI	GCCGGC	1 cut(s)	501
Ksp22I	TGATCA	1 cut(s)	390
Kzo9I	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
LguI	GCTCTTC	2 cut(s)	2409, 2604
Lsp1109I	GCAGC	9 cut(s)	59, 491, 1127, 1993, 3167, 3201, 3604, 3670, 3673
MaeI	CTAG	4 cut(s)	2424, 2568, 3213, 3441
MaeII	ACGT	9 cut(s)	955, 1040, 1251, 1279, 1309, 1434, 2287, 2975, 3500
MaeIII	GTNAC	8 cut(s)	559, 734, 1152, 1430, 2365, 2724, 3394, 3568
MalI	GATC	8 cut(s)	392, 616, 1107, 1214, 1626, 2392, 3359, 3679
MbiI	CCGCTC	4 cut(s)	205, 799, 2419, 2620
MboI	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
MfeI	CAATTG	2 cut(s)	2346, 2594
MflI	RGATCY	1 cut(s)	1212
MhlI	GDGCHC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
MlsI	TGGCCA	3 cut(s)	1346, 2240, 2432
MluNI	TGGCCA	3 cut(s)	1346, 2240, 2432
MlyI	GAGTC	4 cut(s)	1388, 2038, 2090, 3101
MmeI	TCCRAC	2 cut(s)	2916, 3537
Mox20I	TGGCCA	3 cut(s)	1346, 2240, 2432
Mph1103I	ATGCAT	2 cut(s)	2172, 3427
MroNI	GCCGGC	1 cut(s)	499
MroXI	GAANNNNTTC	5 cut(s)	18, 843, 2127, 2278, 2841
MscI	TGGCCA	3 cut(s)	1346, 2240, 2432
MseI	TTAA	8 cut(s)	417, 852, 950, 1043, 1764, 2073, 2136, 3102
MslI	CAYNNNNRTG	3 cut(s)	644, 2919, 3653
Msp20I	TGGCCA	3 cut(s)	1346, 2240, 2432
MspA1I	CMGCKG	3 cut(s)	1019, 3683, 3699
MspCI	CTTAAG	1 cut(s)	2135
MspI	CCGG	7 cut(s)	99, 162, 267, 500, 2944, 3229, 3790
MspR9I	CCNGG	7 cut(s)	99, 110, 268, 2514, 2576, 2716, 2945
MunI	CAATTG	2 cut(s)	2346, 2594
Mva1269I	GAATGC	4 cut(s)	843, 3230, 3427, 3727
MvaI	CCWGG	4 cut(s)	110, 2514, 2576, 2716
MvnI	CGCG	2 cut(s)	2272, 2616
NaeI	GCCGGC	1 cut(s)	501
NciI	CCSGG	3 cut(s)	99, 268, 2945
NcoI	CCATGG	3 cut(s)	223, 1110, 2583
NdeI	CATATG	1 cut(s)	1198
NdeII	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
NgoMIV	GCCGGC	1 cut(s)	499
NlaIV	GGNNCC	2 cut(s)	265, 2648
NmuCI	GTSAC	3 cut(s)	559, 734, 2365
NruI	TCGCGA	1 cut(s)	2272
NsbI	TGCGCA	1 cut(s)	2930
NsiI	ATGCAT	2 cut(s)	2172, 3427
NspI	RCATGY	2 cut(s)	674, 2554
PagI	TCATGA	1 cut(s)	1627
PaqCI	CACCTGC	1 cut(s)	493
PceI	AGGCCT	1 cut(s)	2333
PciSI	GCTCTTC	2 cut(s)	2409, 2604
PctI	GAATGC	4 cut(s)	843, 3230, 3427, 3727
PdiI	GCCGGC	1 cut(s)	501
PdmI	GAANNNNTTC	5 cut(s)	18, 843, 2127, 2278, 2841
PflFI	GACNNNGTC	1 cut(s)	2316
PflMI	CCANNNNNTGG	1 cut(s)	778
PfoI	TCCNGGA	1 cut(s)	266
PleI	GAGTC	4 cut(s)	1388, 2038, 2089, 3100
PmaCI	CACGTG	1 cut(s)	2288
PmlI	CACGTG	1 cut(s)	2288
PpsI	GAGTC	4 cut(s)	1388, 2038, 2089, 3100
Ppu21I	YACGTR	2 cut(s)	1435, 2288
Psp124BI	GAGCTC	1 cut(s)	1689
Psp6I	CCWGG	4 cut(s)	108, 2512, 2574, 2714
PspCI	CACGTG	1 cut(s)	2288
PspGI	CCWGG	4 cut(s)	108, 2512, 2574, 2714
PspN4I	GGNNCC	2 cut(s)	265, 2648
PspPI	GGNCC	4 cut(s)	42, 2318, 2896, 3669
PstI	CTGCAG	1 cut(s)	2326
PstNI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3686
PsuI	RGATCY	1 cut(s)	1212
PsyI	GACNNNGTC	1 cut(s)	2316
PvuII	CAGCTG	2 cut(s)	1019, 3683
RruI	TCGCGA	1 cut(s)	2272
RsaI	GTAC	2 cut(s)	243, 2388
RsaNI	GTAC	2 cut(s)	242, 2387
RseI	CAYNNNNRTG	3 cut(s)	644, 2919, 3653
SacI	GAGCTC	1 cut(s)	1689
SapI	GCTCTTC	2 cut(s)	2409, 2604
SaqAI	TTAA	8 cut(s)	417, 852, 950, 1043, 1764, 2073, 2136, 3102
Sau3AI	GATC	8 cut(s)	390, 614, 1105, 1212, 1624, 2390, 3357, 3677
Sau96I	GGNCC	4 cut(s)	42, 2318, 2896, 3669
SchI	GAGTC	4 cut(s)	1388, 2038, 2090, 3101
ScrFI	CCNGG	7 cut(s)	99, 110, 268, 2514, 2576, 2716, 2945
SduI	GDGCHC	6 cut(s)	641, 1072, 1195, 1315, 1689, 1885
SfcI	CTRYAG	1 cut(s)	2322
SinI	GGWCC	2 cut(s)	2318, 2896
SmiMI	CAYNNNNRTG	3 cut(s)	644, 2919, 3653
SmlI	CTYRAG	2 cut(s)	1965, 2135
SmoI	CTYRAG	2 cut(s)	1965, 2135
SseBI	AGGCCT	1 cut(s)	2333
SspI	AATATT	2 cut(s)	678, 1549
SspMI	CTAG	4 cut(s)	2424, 2568, 3213, 3441
SstI	GAGCTC	1 cut(s)	1689
StuI	AGGCCT	1 cut(s)	2333
StyD4I	CCNGG	7 cut(s)	97, 108, 266, 2512, 2574, 2714, 2943
StyI	CCWWGG	7 cut(s)	223, 330, 891, 1110, 2433, 2583, 3665
TaiI	ACGT	9 cut(s)	958, 1043, 1254, 1282, 1312, 1437, 2290, 2978, 3503
TaqI	TCGA	4 cut(s)	22, 1239, 1282, 3616
TaqII	GACCGA	1 cut(s)	2646
TatI	WGTACW	1 cut(s)	241
TauI	GCSGC	4 cut(s)	924, 1533, 3272, 3702
Tru1I	TTAA	8 cut(s)	417, 852, 950, 1043, 1764, 2073, 2136, 3102
Tru9I	TTAA	8 cut(s)	417, 852, 950, 1043, 1764, 2073, 2136, 3102
TscAI	CASTG	6 cut(s)	465, 537, 568, 1195, 2314, 2494
TseFI	GTSAC	3 cut(s)	559, 734, 2365
TseI	GCWGC	9 cut(s)	72, 479, 1115, 2006, 3180, 3189, 3592, 3683, 3686
Tsp45I	GTSAC	3 cut(s)	559, 734, 2365
TspGWI	ACGGA	5 cut(s)	79, 823, 837, 921, 2230
TspRI	CASTG	6 cut(s)	465, 537, 568, 1195, 2314, 2494
Tth111I	GACNNNGTC	1 cut(s)	2316
Van91I	CCANNNNNTGG	1 cut(s)	778
Vha464I	CTTAAG	1 cut(s)	2135
VneI	GTGCAC	1 cut(s)	1191
VpaK11BI	GGWCC	2 cut(s)	2318, 2896
XceI	RCATGY	2 cut(s)	674, 2554
XcmI	CCANNNNNNNNNTGG	1 cut(s)	116
XmnI	GAANNNNTTC	5 cut(s)	18, 843, 2127, 2278, 2841
XspI	CTAG	4 cut(s)	2424, 2568, 3213, 3441
Zsp2I	ATGCAT	2 cut(s)	2172, 3427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.