Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001895

Physical Location & Seq

Genomic Coordinates Reverse (-)

10383809 .. 10390559

Gene Structure 6751 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001104

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGACTAAAGCTCGGACTCCTGCAACAACATTGGTGACTTGGAGACTTGTTGACTGTGTAGAGGCGGATGGTGATGACTTTCACTCAAACAAAATATTATTCCCCTGTAATGATGAATATTCATCTGATTACGATACCAAAGCTAGGGATGTTTCTGATGTGATAGATGTAGAAAAAGAGCAAGAACATTCTTCTCTTCAAGAAAAACTGGATCGGGAGCTGAAAGAATTGGACAAGCCTCTCGTGCAAAAGGAGGCTAAAATGAAGTGGTTTACAAGTTCTGATACCTCGGTTCTTAAGCTGTATGAGAAGAAGGTTCATGAGTTAGAACATGAGAAGAAAGCCCTTCAGAGAGAGGTTGAAGAGTTGAGACAAAATCTTTCAAATATCTCATCTACTTCTGGGGATGGAGCTCAAAAGTTAAAGGAGGATTATCTACACAAGTTGAACCTTCTTGAAGGACAGGTTTCTGAGCTGAAAAAGAAACAGGATGCTCAAGCCCAATTGTTGAGACAAAAACAAAAAACCGATGAGGCTGTTCAATTGCAACACAAGATCAAACAGGAGTCTGAGCAGTTTAGATTATGGAAAGCATCACGAGAAAAAGAAGTCCTTCAGAGGGAAGGAGATGAGATGCATAAGCTGTTAGCTTTAAACCAGAGGCAAAAAATGGTTTTGCAACGAAAGACAGAAGAAGCTAATATGGCCACAAAAAAGCTAAAGGAGCTTTTAGAATCCAGAAAGACTTCTTCACTGAAACTTCTGGCTCTGATGCTGGCAATCGAACTTGAGCTTGAAGTCACAGTTAAGACTCATGAAGTAAGATCTGAGTATGAGCGGCAAATTGAAGAGCGTGCTATGATGGCCAAGGAAAGAACCAAGCTAATAGAAGAACACCGTAATTTAAGGTGCCTGTTACGAGATAAAGAAGTTGCATACAGAGAGAAGGATTTGGAAATAAGAGATTTGAAGGAAAAAGTTGTAAGCCTCAGTAGTTCGCTCAGGAAGTTGGAGAATAAGAATGCTGAACTTATGCATCAGGGATTCAATGCGCAAAGTACTAATGACTCTGGAGGCTTCAAGTTAGATAGTGCTTGTGAGCGGATAGTCAGTGTTGTGAAGAAAAGCGAGTCCGGCGTGTGCTGCTCTTGTAGCAAGACTTCCTCCTGCAAAACAACTAAATGTCAATGTCAAACTACTGGAGGAGCCTGTGGGCCATCATGTGGTTGTGTGCCTGCTAAGTGTTCAAATAGAGAATCCAGCCTTGAGGTACATGGGTCTCCAGAAGAAGAAACTGCTCAGGGAATTGGAAATGATGAAGGGACTGATGAAACAGAGAAGAATCAGCATCTTGCTTCTCAGGGTGCCAGGTTACTTCAGAATGCATTAGTTGAGAGGCCTAGTGAAACAACTGATGATGGTGGACCAAGAAGGAAAGCTCTTTCAGAGATCGGAAATGCGTTGGCCAAATCCAATGCACCGCAGCCTAACCAGAGAAAGAAATGGCGAAGAACCACAATCCAACTGGTTACAAATGCTCCGCCTCCCTCGCAGCCAGCAACTGCTGAAGCACCTAAAAGGGCAGATAAAGGGGCACCAGAGGTACCCATCCCTATGAAGCTTCCTCAAGGGGCAGATCAAGGGGCACCAGAGGTACCCATCTCTTTGAAGCTTCCTAAAAGGCCAGAAAAAGGGGCACCAGAGGCACCCATCCCTATGAAGCTTCCTAGGGCAATGCGATCAGCTGCATCAAATGGTGGCAACTTATTTAGAGAAAGGAATTCTGATAAAGCAGATCAATCTGGTGTCAATAAGGAGGCCGGTGTTCCTGCTCCAAGAAGTCCTCTGCGGCAGAACAAAACATCAGATGAGAAGGAGAACTGTGGCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0008574 GO:0009506 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0055044 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

631

Amino Acids

70.61

Weight (kDa)

8.43

Isoelectric Point (pI)

51.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KIF21A_4th	PF25764	50 - 206	7.5e-33	KIF21A-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60930 AT5G60930 AT5G60930
fragaria_vesca	FvH4_5g35340
malus_domestica	MD08G1198000.v1.1 MD15G1385300.v1.1
prunus_persica	Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1
pyrus_communis	pycom08g17020 pycom15g07240 pycom15g34540
rosa_chinensis	RchiOBHm_Chr3g0494071 RchiOBHm_Chr7g0236541 RchiOBHm_Chr7g0236581 RchiOBHm_Chr7g0236691
rosa_laevigata	RLG00000000987 RLG00000000988 RLG00000001068 RLG00000001077 RLG00000001104
rosa_multiflora	Rmu_sc0002536.1_g000016 Rmu_sc0003720.1_g000010 Rmu_sc0011298.1_g000005 Rmu_ssc0000050.1_g000117
rosa_roxburghii	Rroxscaffold_3G00224960 Rroxscaffold_6G00429280
rosa_rugosa	Rorug07G0291900 Rorug07G0291900 Rorug07G0297600 Rorug07G0297700 Rorug07G0298200
rosa_samantha	Rh2CG391900 Rh7AG453300 Rh7AG453600 Rh7BG401100 Rh7BG418700 Rh7BG424500 Rh7CG471900 Rh7CG472100 Rh7DG438700 Rh7DG440900 Rh7DG441000
rosa_wichuraiana	Rw0G020690 Rw7G037620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1053
Acc65I	GGTACC	2 cut(s)	1603, 1654
AccB1I	GGYRCC	8 cut(s)	909, 1364, 1594, 1603, 1645, 1654, 1696, 1705
AccB7I	CCANNNNNTGG	1 cut(s)	1223
AccBSI	CCGCTC	2 cut(s)	838, 1102
AciI	CCGC	6 cut(s)	65, 838, 1102, 1481, 1541, 1849
AclWI	GGATC	1 cut(s)	219
AcoI	YGGCCR	3 cut(s)	705, 864, 1464
AcsI	RAATTY	1 cut(s)	1780
AcuI	CTGAAG	4 cut(s)	332, 599, 1361, 1587
AfaI	GTAC	4 cut(s)	1060, 1272, 1605, 1656
AfiI	CCNNNNNNNGG	4 cut(s)	144, 619, 1223, 1691
AflII	CTTAAG	1 cut(s)	296
AjnI	CCWGG	1 cut(s)	1367
Alw21I	GWGCWC	1 cut(s)	415
Alw26I	GTCTC	4 cut(s)	37, 364, 505, 1284
AlwI	GGATC	1 cut(s)	219
AlwNI	CAGNNNCTG	1 cut(s)	1562
AoxI	GGCC	8 cut(s)	705, 864, 1214, 1397, 1464, 1682, 1818, 1885
ApeKI	GCWGC	4 cut(s)	1143, 1483, 1552, 1745
ApoI	RAATTY	1 cut(s)	1780
Asp700I	GAANNNNTTC	2 cut(s)	612, 745
Asp718I	GGTACC	2 cut(s)	1603, 1654
AspA2I	CCTAGG	1 cut(s)	1727
AspLEI	GCGC	1 cut(s)	1054
AspS9I	GGNCC	2 cut(s)	1214, 1424
AsuHPI	GGTGA	2 cut(s)	46, 83
AvaII	GGWCC	1 cut(s)	1424
AvrII	CCTAGG	1 cut(s)	1727
BaeGI	GKGCMC	3 cut(s)	1597, 1648, 1699
BaeI	ACNNNNGTAYC	4 cut(s)	276, 309, 1262, 1295
BalI	TGGCCA	3 cut(s)	707, 866, 1466
BanI	GGYRCC	8 cut(s)	909, 1364, 1594, 1603, 1645, 1654, 1696, 1705
BanII	GRGCYC	1 cut(s)	415
BarI	GAAGNNNNNNTAC	2 cut(s)	1144, 1176
BauI	CACGAG	2 cut(s)	242, 597
Bbv12I	GWGCWC	1 cut(s)	415
BbvI	GCAGC	4 cut(s)	1130, 1495, 1564, 1732
BccI	CCATC	8 cut(s)	62, 401, 856, 1225, 1412, 1616, 1667, 1718
BciT130I	CCWGG	1 cut(s)	1369
BcoDI	GTCTC	4 cut(s)	37, 364, 505, 1284
BfaI	CTAG	3 cut(s)	144, 1401, 1728
BfrI	CTTAAG	1 cut(s)	296
BglII	AGATCT	1 cut(s)	824
BisI	GCNGC	6 cut(s)	839, 1144, 1484, 1553, 1746, 1850
BlnI	CCTAGG	1 cut(s)	1727
BlsI	GCNGC	6 cut(s)	840, 1145, 1485, 1554, 1747, 1851
BmcAI	AGTACT	1 cut(s)	1060
Bme1390I	CCNGG	1 cut(s)	1369
Bme18I	GGWCC	1 cut(s)	1424
BmgT120I	GGNCC	2 cut(s)	1214, 1424
BmiI	GGNNCC	9 cut(s)	911, 1207, 1366, 1596, 1605, 1647, 1656, 1698, 1707
BmrFI	CCNGG	1 cut(s)	1369
BmsI	GCATC	7 cut(s)	481, 602, 624, 762, 1045, 1357, 1757
BpmI	CTGGAG	3 cut(s)	1092, 1221, 1266
Bpu10I	CCTNAGC	2 cut(s)	1001, 1299
BpuEI	CTTGAG	4 cut(s)	480, 809, 1286, 1611
BsaI	GGTCTC	1 cut(s)	1284
BsaJI	CCNNGG	3 cut(s)	288, 867, 1727
BsaXI	ACNNNNNCTCC	2 cut(s)	1522, 1552
Bsc4I	CCNNNNNNNGG	4 cut(s)	144, 619, 1223, 1691
Bse118I	RCCGGY	1 cut(s)	1820
Bse1I	ACTGG	3 cut(s)	213, 1204, 1530
Bse3DI	GCAATG	1 cut(s)	1740
BseBI	CCWGG	1 cut(s)	1369
BseDI	CCNNGG	3 cut(s)	288, 867, 1727
BseGI	GGATG	6 cut(s)	73, 154, 412, 496, 1608, 1710
BseLI	CCNNNNNNNGG	4 cut(s)	144, 619, 1223, 1691
BseMI	GCAATG	1 cut(s)	1740
BseMII	CTCAG	7 cut(s)	462, 561, 819, 1003, 1015, 1313, 1373
BseNI	ACTGG	3 cut(s)	213, 1204, 1530
BseRI	GAGGAG	1 cut(s)	1218
BseSI	GKGCMC	3 cut(s)	1597, 1648, 1699
BseXI	GCAGC	4 cut(s)	1130, 1495, 1564, 1732
BshFI	GGCC	8 cut(s)	707, 866, 1216, 1399, 1466, 1684, 1820, 1887
BshNI	GGYRCC	8 cut(s)	909, 1364, 1594, 1603, 1645, 1654, 1696, 1705
BsiHKAI	GWGCWC	1 cut(s)	415
BsiSI	CCGG	2 cut(s)	1134, 1821
BslFI	GGGAC	1 cut(s)	1336
BslI	CCNNNNNNNGG	4 cut(s)	144, 619, 1223, 1691
BsmAI	GTCTC	4 cut(s)	37, 364, 505, 1284
BsmFI	GGGAC	1 cut(s)	1336
BsmI	GAATGC	2 cut(s)	1027, 1387
BsnI	GGCC	8 cut(s)	707, 866, 1216, 1399, 1466, 1684, 1820, 1887
Bso31I	GGTCTC	1 cut(s)	1284
Bsp1286I	GDGCHC	4 cut(s)	415, 1597, 1648, 1699
Bsp143I	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
BspACI	CCGC	6 cut(s)	65, 838, 1102, 1481, 1541, 1849
BspANI	GGCC	8 cut(s)	707, 866, 1216, 1399, 1466, 1684, 1820, 1887
BspCNI	CTCAG	7 cut(s)	463, 562, 820, 1002, 1014, 1312, 1372
BspHI	TCATGA	2 cut(s)	319, 814
BspLI	GGNNCC	9 cut(s)	911, 1207, 1366, 1596, 1605, 1647, 1656, 1698, 1707
BspPI	GGATC	1 cut(s)	219
BspQI	GCTCTTC	1 cut(s)	843
BspT107I	GGYRCC	8 cut(s)	909, 1364, 1594, 1603, 1645, 1654, 1696, 1705
BspTI	CTTAAG	1 cut(s)	296
BspTNI	GGTCTC	1 cut(s)	1284
BsrBI	CCGCTC	2 cut(s)	838, 1102
BsrDI	GCAATG	1 cut(s)	1740
BsrFI	RCCGGY	1 cut(s)	1820
BsrI	ACTGG	3 cut(s)	213, 1204, 1530
BssAI	RCCGGY	1 cut(s)	1820
BssECI	CCNNGG	3 cut(s)	288, 867, 1727
BssMI	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
BssSI	CACGAG	2 cut(s)	242, 597
BssT1I	CCWWGG	2 cut(s)	867, 1727
Bst2BI	CACGAG	2 cut(s)	242, 597
Bst2UI	CCWGG	1 cut(s)	1369
Bst4CI	ACNGT	4 cut(s)	56, 805, 899, 1883
Bst6I	CTCTTC	3 cut(s)	201, 357, 843
BstAFI	CTTAAG	1 cut(s)	296
BstC8I	GCNNGC	4 cut(s)	777, 855, 1236, 1557
BstDEI	CTNAG	8 cut(s)	471, 570, 828, 989, 1001, 1239, 1299, 1359
BstF5I	GGATG	6 cut(s)	73, 154, 412, 496, 1608, 1710
BstHHI	GCGC	1 cut(s)	1054
BstKTI	GATC	7 cut(s)	214, 558, 827, 1452, 1639, 1742, 1798
BstMAI	GTCTC	4 cut(s)	37, 364, 505, 1284
BstMBI	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
BstMWI	GCNNNNNNNGC	9 cut(s)	244, 704, 724, 863, 1134, 1143, 1152, 1549, 1703
BstNI	CCWGG	1 cut(s)	1369
BstSCI	CCNGG	1 cut(s)	1367
BstSLI	GKGCMC	3 cut(s)	1597, 1648, 1699
BstV1I	GCAGC	4 cut(s)	1130, 1495, 1564, 1732
BstX2I	RGATCY	1 cut(s)	824
BstYI	RGATCY	1 cut(s)	824
BsuRI	GGCC	8 cut(s)	707, 866, 1216, 1399, 1466, 1684, 1820, 1887
BtsCI	GGATG	6 cut(s)	73, 154, 412, 496, 1608, 1710
BtsIMutI	CAGTG	2 cut(s)	752, 1117
Cac8I	GCNNGC	4 cut(s)	777, 855, 1236, 1557
CaiI	CAGNNNCTG	1 cut(s)	1562
CciI	TCATGA	2 cut(s)	319, 814
CfoI	GCGC	1 cut(s)	1054
Cfr10I	RCCGGY	1 cut(s)	1820
Cfr13I	GGNCC	2 cut(s)	1214, 1424
Csp6I	GTAC	4 cut(s)	1059, 1271, 1604, 1655
CviAII	CATG	5 cut(s)	320, 332, 815, 1221, 1274
CviQI	GTAC	4 cut(s)	1059, 1271, 1604, 1655
DdeI	CTNAG	8 cut(s)	471, 570, 828, 989, 1001, 1239, 1299, 1359
DpnI	GATC	7 cut(s)	213, 557, 826, 1451, 1638, 1741, 1797
DpnII	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
DraI	TTTAAA	1 cut(s)	654
EaeI	YGGCCR	3 cut(s)	705, 864, 1464
Eam1104I	CTCTTC	3 cut(s)	201, 357, 843
EarI	CTCTTC	3 cut(s)	201, 357, 843
EciI	GGCGGA	2 cut(s)	80, 1530
Ecl136II	GAGCTC	1 cut(s)	413
Eco130I	CCWWGG	2 cut(s)	867, 1727
Eco147I	AGGCCT	1 cut(s)	1399
Eco24I	GRGCYC	1 cut(s)	415
Eco31I	GGTCTC	1 cut(s)	1284
Eco47I	GGWCC	1 cut(s)	1424
Eco53kI	GAGCTC	1 cut(s)	413
Eco57I	CTGAAG	4 cut(s)	332, 599, 1361, 1587
EcoICRI	GAGCTC	1 cut(s)	413
EcoRI	GAATTC	1 cut(s)	1780
EcoRII	CCWGG	1 cut(s)	1367
EcoT14I	CCWWGG	2 cut(s)	867, 1727
EcoT22I	ATGCAT	3 cut(s)	639, 1038, 1387
EcoT38I	GRGCYC	1 cut(s)	415
ErhI	CCWWGG	2 cut(s)	867, 1727
FaeI	CATG	5 cut(s)	323, 335, 818, 1224, 1277
FalI	AAGNNNNNCTT	2 cut(s)	597, 629
FaqI	GGGAC	1 cut(s)	1336
FatI	CATG	5 cut(s)	319, 331, 814, 1220, 1273
Fnu4HI	GCNGC	6 cut(s)	839, 1144, 1484, 1553, 1746, 1850
FokI	GGATG	6 cut(s)	80, 161, 419, 503, 1595, 1697
FriOI	GRGCYC	1 cut(s)	415
Fsp4HI	GCNGC	6 cut(s)	839, 1144, 1484, 1553, 1746, 1850
FspBI	CTAG	3 cut(s)	144, 1401, 1728
FspI	TGCGCA	1 cut(s)	1053
GlaI	GCGC	1 cut(s)	1053
GluI	GCNGC	6 cut(s)	839, 1144, 1484, 1553, 1746, 1850
GsuI	CTGGAG	3 cut(s)	1092, 1221, 1266
HaeIII	GGCC	8 cut(s)	707, 866, 1216, 1399, 1466, 1684, 1820, 1887
HapII	CCGG	2 cut(s)	1134, 1821
HhaI	GCGC	1 cut(s)	1054
Hin1II	CATG	5 cut(s)	323, 335, 818, 1224, 1277
Hin6I	GCGC	1 cut(s)	1052
HinP1I	GCGC	1 cut(s)	1052
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	3 cut(s)	1619, 1670, 1721
HinfI	GANTC	9 cut(s)	16, 566, 734, 811, 1044, 1067, 1130, 1256, 1342
HpaII	CCGG	2 cut(s)	1134, 1821
HphI	GGTGA	2 cut(s)	46, 83
Hpy166II	GTNNAC	3 cut(s)	52, 273, 1424
Hpy8I	GTNNAC	3 cut(s)	52, 273, 1424
HpyCH4III	ACNGT	4 cut(s)	56, 805, 899, 1883
HpyF10VI	GCNNNNNNNGC	9 cut(s)	244, 704, 724, 863, 1134, 1143, 1152, 1549, 1703
HpyF3I	CTNAG	8 cut(s)	471, 570, 828, 989, 1001, 1239, 1299, 1359
Hsp92II	CATG	5 cut(s)	323, 335, 818, 1224, 1277
HspAI	GCGC	1 cut(s)	1052
KpnI	GGTACC	2 cut(s)	1607, 1658
Kzo9I	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
LguI	GCTCTTC	1 cut(s)	843
LmnI	GCTCC	6 cut(s)	217, 410, 724, 1205, 1543, 1837
Lsp1109I	GCAGC	4 cut(s)	1130, 1495, 1564, 1732
LweI	GCATC	7 cut(s)	481, 602, 624, 762, 1045, 1357, 1757
MaeI	CTAG	3 cut(s)	144, 1401, 1728
MaeIII	GTNAC	5 cut(s)	34, 799, 915, 1371, 1528
MalI	GATC	7 cut(s)	213, 557, 826, 1451, 1638, 1741, 1797
MbiI	CCGCTC	2 cut(s)	838, 1102
MboI	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
MfeI	CAATTG	2 cut(s)	503, 542
MflI	RGATCY	1 cut(s)	824
MhlI	GDGCHC	4 cut(s)	415, 1597, 1648, 1699
MlsI	TGGCCA	3 cut(s)	707, 866, 1466
MluCI	AATT	7 cut(s)	227, 503, 542, 843, 901, 1305, 1780
MluNI	TGGCCA	3 cut(s)	707, 866, 1466
MlyI	GAGTC	5 cut(s)	10, 575, 805, 1061, 1139
MmeI	TCCRAC	2 cut(s)	990, 1546
Mox20I	TGGCCA	3 cut(s)	707, 866, 1466
Mph1103I	ATGCAT	3 cut(s)	639, 1038, 1387
MroXI	GAANNNNTTC	2 cut(s)	612, 745
MscI	TGGCCA	3 cut(s)	707, 866, 1466
MseI	TTAA	6 cut(s)	297, 422, 653, 807, 905, 1891
MslI	CAYNNNNRTG	2 cut(s)	1613, 1715
Msp20I	TGGCCA	3 cut(s)	707, 866, 1466
MspA1I	CMGCKG	1 cut(s)	1745
MspCI	CTTAAG	1 cut(s)	296
MspI	CCGG	2 cut(s)	1134, 1821
MspR9I	CCNGG	1 cut(s)	1369
MunI	CAATTG	2 cut(s)	503, 542
Mva1269I	GAATGC	2 cut(s)	1027, 1387
MvaI	CCWGG	1 cut(s)	1369
MwoI	GCNNNNNNNGC	9 cut(s)	244, 704, 724, 863, 1134, 1143, 1152, 1549, 1703
NdeII	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
NlaIII	CATG	5 cut(s)	323, 335, 818, 1224, 1277
NlaIV	GGNNCC	9 cut(s)	911, 1207, 1366, 1596, 1605, 1647, 1656, 1698, 1707
NmuCI	GTSAC	2 cut(s)	34, 799
NsbI	TGCGCA	1 cut(s)	1053
NsiI	ATGCAT	3 cut(s)	639, 1038, 1387
PagI	TCATGA	2 cut(s)	319, 814
PceI	AGGCCT	1 cut(s)	1399
PciSI	GCTCTTC	1 cut(s)	843
PctI	GAATGC	2 cut(s)	1027, 1387
PdmI	GAANNNNTTC	2 cut(s)	612, 745
PfeI	GAWTC	4 cut(s)	734, 1044, 1256, 1342
PflMI	CCANNNNNTGG	1 cut(s)	1223
PkrI	GCNGC	6 cut(s)	840, 1145, 1485, 1554, 1747, 1851
PleI	GAGTC	5 cut(s)	10, 574, 805, 1061, 1138
PpsI	GAGTC	5 cut(s)	10, 574, 805, 1061, 1138
Psp124BI	GAGCTC	1 cut(s)	415
Psp6I	CCWGG	1 cut(s)	1367
PspGI	CCWGG	1 cut(s)	1367
PspN4I	GGNNCC	9 cut(s)	911, 1207, 1366, 1596, 1605, 1647, 1656, 1698, 1707
PspPI	GGNCC	2 cut(s)	1214, 1424
PstNI	CAGNNNCTG	1 cut(s)	1562
PsuI	RGATCY	1 cut(s)	824
PvuII	CAGCTG	1 cut(s)	1745
RsaI	GTAC	4 cut(s)	1060, 1272, 1605, 1656
RsaNI	GTAC	4 cut(s)	1059, 1271, 1604, 1655
RseI	CAYNNNNRTG	2 cut(s)	1613, 1715
SacI	GAGCTC	1 cut(s)	415
SapI	GCTCTTC	1 cut(s)	843
SaqAI	TTAA	6 cut(s)	297, 422, 653, 807, 905, 1891
SatI	GCNGC	6 cut(s)	839, 1144, 1484, 1553, 1746, 1850
Sau3AI	GATC	7 cut(s)	211, 555, 824, 1449, 1636, 1739, 1795
Sau96I	GGNCC	2 cut(s)	1214, 1424
ScaI	AGTACT	1 cut(s)	1060
SchI	GAGTC	5 cut(s)	10, 575, 805, 1061, 1139
ScrFI	CCNGG	1 cut(s)	1369
SduI	GDGCHC	4 cut(s)	415, 1597, 1648, 1699
SfaNI	GCATC	7 cut(s)	481, 602, 624, 762, 1045, 1357, 1757
SinI	GGWCC	1 cut(s)	1424
SmiMI	CAYNNNNRTG	2 cut(s)	1613, 1715
SmlI	CTYRAG	5 cut(s)	296, 495, 788, 1265, 1626
SmoI	CTYRAG	5 cut(s)	296, 495, 788, 1265, 1626
Sse9I	AATT	7 cut(s)	227, 503, 542, 843, 901, 1305, 1780
SseBI	AGGCCT	1 cut(s)	1399
SsiI	CCGC	6 cut(s)	65, 838, 1102, 1481, 1541, 1849
SspI	AATATT	2 cut(s)	96, 119
SspMI	CTAG	3 cut(s)	144, 1401, 1728
SstI	GAGCTC	1 cut(s)	415
StuI	AGGCCT	1 cut(s)	1399
StyD4I	CCNGG	1 cut(s)	1367
StyI	CCWWGG	2 cut(s)	867, 1727
TaaI	ACNGT	4 cut(s)	56, 805, 899, 1883
TaqI	TCGA	1 cut(s)	783
TasI	AATT	7 cut(s)	227, 503, 542, 843, 901, 1305, 1780
TatI	WGTACW	1 cut(s)	1058
TauI	GCSGC	2 cut(s)	841, 1852
TfiI	GAWTC	4 cut(s)	734, 1044, 1256, 1342
Tru1I	TTAA	6 cut(s)	297, 422, 653, 807, 905, 1891
Tru9I	TTAA	6 cut(s)	297, 422, 653, 807, 905, 1891
TscAI	CASTG	2 cut(s)	759, 1117
TseFI	GTSAC	2 cut(s)	34, 799
TseI	GCWGC	4 cut(s)	1143, 1483, 1552, 1745
Tsp45I	GTSAC	2 cut(s)	34, 799
TspDTI	ATGAA	9 cut(s)	111, 129, 278, 308, 831, 1332, 1344, 1631, 1733
TspRI	CASTG	2 cut(s)	759, 1117
Van91I	CCANNNNNTGG	1 cut(s)	1223
Vha464I	CTTAAG	1 cut(s)	296
VpaK11BI	GGWCC	1 cut(s)	1424
XapI	RAATTY	1 cut(s)	1780
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1522
XmaJI	CCTAGG	1 cut(s)	1727
XmnI	GAANNNNTTC	2 cut(s)	612, 745
XspI	CTAG	3 cut(s)	144, 1401, 1728
ZrmI	AGTACT	1 cut(s)	1060
Zsp2I	ATGCAT	3 cut(s)	639, 1038, 1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.