Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001895

Physical Location & Seq

Genomic Coordinates Forward (+)

47600342 .. 47610538

Gene Structure 10197 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1385300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3840 bp

ATGGATAGTTCGGAATGTGTTCAAGTGGCTGTCAATGTGAGGCCCTTGATTACCTCCGAGCTTCTGGTAGGCTGCACAGATTGCATTTCCGTCGTTCCCGGTGAACCCCAGGTGCAAATTGGGACACATTCATTCACATATGATTATGTGTATGGTAGCTCCGGACTTCCTTCCAATGCGGTGTATGACGATTGTGTTGCTCCGCTTGTTGATGCCCTTTTCCATGGATACAATGCGACTGTACTCGCTTATGGACAGACGGGTTCCGGTAAGACATATACAATGGGGACTAACTATACTGGAGAAGGAAGTAATGGTGGAATAGTACCCAAAGTGATGGAAAGTATATTTAAGAAAGTGGAGACAACGAAAGATAACACGGAGTTTTTGATCAGGGTATCATTTATTGAGATATTTAAGGAGGAAGTATTTGATTTGCTTGACCCAAATTCATCATCACTCCACAAAAACGAAGGGGCAGCACCTGCAAAGCCTGTCCCAGCAAGAGTGCCTATACAAATTAGAGAAACAGTGAATGGAGGGATAACTCTTGCTGGTGTCACTGAGGCAGAGGTTAGAACAAAAGAAGAGATGGCATCATATCTCACTCGTGGATCATTATCTCGTGCTACTGGGAGCACCAATATGAACAGCCAGTCAAGCCGTTCACATGCAATATTTACAATAACTATGGAGCAGAAGAGGATAGCTCACTCTCTGAATGGAGCAAATAATGATGATATTGGTGATGATATCTTATGCGCCAAGCTCCATTTAGTGGATTTAGCAGGGTCTGAGCGGGCAAAACGGACAGGTGCTGACGGAATGCGGTTGAAAGAAGGCATTCATATCAACAAGGGTTTACTTGCTCTTGGCAATGTGATAAGTGCCTTGGGAGATGAAAAGAAGCGGAAAGAAGGCGGCCATGTTCCATATCGTGATAGCAAATTAACACGTTTGTTACAGGATTCACTCGGAGGAAATAGTAAGACAGTGATGATTGCTTGTGTTAGTCCAGCTGACACAAATGCTGAAGAGACGTTGAATACTTTGAAATATGCTAATCGTGCTCGCAACATCCAGAATAAGGCAGTTGTCAATCGTGATCCCATGGCAGCTCAGTTGCAAACAATGAGAAGCCAGATTGAACAGTTACAGACTGAACTTTTATTTTATCGTGGCGATGCTAGTGCACCATATGAGGAACTTCAGATCCTGAAGCACAAAGTATCGTTACTCGAAGAAAGCAATGTGGAATTGCGTCACGAGCTTCAAGAACGTCGAGTTTCATGTGAACATCTAAAGCAACGTGCTTTTGAGGCTCAGGTTGAAAAAGATAAACTGGCCATGATAATTGAATCAGCACGAAGTGGTAAACCTTGGGATGAGATTGACTCGAATTCAGTTCAGGATTATGACTTGCTAAAAGGTTATGTGTCAAAAATTCAGGAGTTAGAAGGGGAATTATTGTGCTTGAAAAAATCAAACAACTCAAAGCACAAGAGACTTGTTGAGTGTGCTGAGTCAGATGATGACAGCTTTCGCTCTAAGAATATATTATTCCCCTCTATCAATGAATATTCATCTGATTATGATACTAAAGCTGGGGATATTTCAGTTGACGAGATTGAAGATCACGAAAAGGAGCAAAAGGAGCAAGAATTTTCTTCTCTTCAAGAAAAATTTGACAGGGAGCTCAAAGAATTGGACAAAGCTCTTGAACAGAAGGAGGCTGAAATGAAGCGGTTTACTACTTCTGATACCTCGGTTCTTAAACTACATTATGAGAAGAAAGTTCAGGAGTTAGAGCATGAAAAGAAAACACTGCAGAAAGAGATTGAGGAGTTGAGACACAATCTTTCAAATATATCATCTACTTCTGATGATGGTGCTCAAAAGTTGAAAGAAGATTATCTACACAAGTTGAATGTCCTTGAAGGGCAGGTTTCTGAGCTCAAAAAGAAGCAAGATGCTCAAGCTCAACTCTTGAGACAAAAACAAAAAAGTGATGAGGCTGCAAAGCGACTTCAGGATGAAATACAGAGAATAAAGACTCAAAAGGTTCAACTGCAACACAAGATCAAACAAGAGTCTGAGCAGTTTAGATTATGGAAAACATCACGAGAAAAAGAAGTTCTTCAGCTTAAGAAAGAGGGGAGGAGGAATGAGTATGAAATGCATAAACTATTAGCTTTGAATCAGAGGCAAAAAATGGTTTTGCAACGGAAGACAGAAGAAGCTTCTATGGCCACAAAAAGGCTAAAAGAGCTTTTAGAATCGCGAAAGACTTCTTCACGTGAAACTTTTGGAGCTGGCAGTGGACATGGTCCTGGAGTTCAGGCTTTGATGCAGGCAATTGAACATGAGCTTGAAGTCACAGTTCGGGTGCATGAAGTACGTTCTGAGTATGAGCGTCAAATGGAAGAGCGGGCTAGGATGGCCAAGGAGGTTGCAAAGCTAAAAGAAGAAGCAGAAATGTTAAAACGAACTAATTTCAGCGATTACCCTGAATCTATGTCTCCTGGTGCAAGAAATTCGAGGATTTATGCACTTGAAAACATGCTTGCTACTTCATCTAGCACCCTGGTTTCTATGGCATCACAATTATCAGAAGCAGAAGAGCGCGAGCGGGGTTTTACTGGGAGGGCACGCTGGAACCAAGTTCGGTCTCTTGCTGATGCAAAAAATGTGATGAATCATTTGTTCAATTTAGCTTCTTCCTCCAGGTGCTTGTTGCGAGATAAAGAAGTTGCATACAGAGAGAGGGATTTGGAAATAAGAGATTTGAAGGAAAAAGTTGTAAGCTTGAGTCGTTTCCTTAGAAAATCAGAGATGCAGAGGTCTGAACTTGTTCATCAGAATGCAGCCTTGAAGAAATTTGCCATGAACTGTTCCAAGGATGCGGACCTAAACAATGGTGGACACAAGTATGATTTGCGCAAACTGGAAAACCGGGCATCGTTTATCTGGGAGGATATGGATACGTCTGATTCAGAAAAATCTGAAGCGGAGGATGGCGACTGGGTGATGTCAGGAAAACGACCATCTAAAAAGAGAAAATCCAAAAGTGGAAGTTCAAGTGGGGGAGGATATAATGAGTCAGAAATTAAGAATGTTGGAGGCTTCAAATTAGATGCTTCAGGTGAGGGGATTGTGTCTGTGAAGAAAAGTGAATCAGGCGTATGCTGCTCTTGCAGCAAAAGCTCCTCATGCAAAACTAGCAAATGCGAATGCCGGTCTTCCAGAGGCACTTGTGGGTCATCATGTGGCTGCGCGGCTGCAAAGTGTTCAAATAGAGAATCAGCTTCACTTGTGGCGCAAGAATCTGCACACGCGGAGGTGTCTGAAGTGATTAGAAACGAGATAGGGACTGATGAAGAAGAGAAGAATCAGATTCTTGTTACTCATGGTGCAAGGTTGCTTCAGAATGCATTAGCTGATAACTCTAGCGAGACAACTGATGATGGTGAACTAAGAAGGAAGGCTCTTTCCGAAATTGGAAATACGTTGGTCAAATCCAACGCACCAAAGCCTAACCAGAGAAAGAAGTGGCGAAAATCCACAATTCAACTTGTTACAAATGCTCCGCCTCCCCCGCAGCCAGAAGTTGCTGAAGCACCTCGAAGGCCAGATAACAAAGGACATGAGGCAAGCATCCCTATGAAGCTACCAAGGGCCATGCGATCAGTTGCTGCATCAAACGTTGGCAACCCATTTAGAGAAAGGAATGCCGAAAAACCTGAACAATCGGGCGCCAACAAGGAAGCTGGAATTCCTGCGGCACCAACAAGTCCTCTCCGGCAGAAGAGAACAACAGATGAGAAGGAAAACCACCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0008574 GO:0009506 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0055044 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1280

Amino Acids

143.01

Weight (kDa)

7.34

Isoelectric Point (pI)

51.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	37 - 147	1e-17	Microtubule binding
Kinesin	PF00225	38 - 360	4e-105	Kinesin motor domain
KIF21A_4th	PF25764	541 - 718	6e-56	KIF21A-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60930 AT5G60930 AT5G60930
fragaria_vesca	FvH4_5g35340
malus_domestica	MD08G1198000.v1.1 MD15G1385300.v1.1
prunus_persica	Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1 Prupe.1G532000_v2.0.a1
pyrus_communis	pycom08g17020 pycom15g07240 pycom15g34540
rosa_chinensis	RchiOBHm_Chr3g0494071 RchiOBHm_Chr7g0236541 RchiOBHm_Chr7g0236581 RchiOBHm_Chr7g0236691
rosa_laevigata	RLG00000000987 RLG00000000988 RLG00000001068 RLG00000001077 RLG00000001104
rosa_multiflora	Rmu_sc0002536.1_g000016 Rmu_sc0003720.1_g000010 Rmu_sc0011298.1_g000005 Rmu_ssc0000050.1_g000117
rosa_roxburghii	Rroxscaffold_3G00224960 Rroxscaffold_6G00429280
rosa_rugosa	Rorug07G0291900 Rorug07G0291900 Rorug07G0297600 Rorug07G0297700 Rorug07G0298200
rosa_samantha	Rh2CG391900 Rh7AG453300 Rh7AG453600 Rh7BG401100 Rh7BG418700 Rh7BG424500 Rh7CG471900 Rh7CG472100 Rh7DG438700 Rh7DG440900 Rh7DG441000
rosa_wichuraiana	Rw0G020690 Rw7G037620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	493
Acc16I	TGCGCA	1 cut(s)	2939
Acc36I	ACCTGC	2 cut(s)	493, 1933
AccB1I	GGYRCC	2 cut(s)	3752, 3781
AccB7I	CCANNNNNTGG	1 cut(s)	778
AccBSI	CCGCTC	3 cut(s)	799, 2428, 2629
AccII	CGCG	4 cut(s)	2281, 2625, 3275, 3335
AccIII	TCCGGA	1 cut(s)	161
AclI	AACGTT	1 cut(s)	3702
AclWI	GGATC	3 cut(s)	622, 1100, 1207
AcoI	YGGCCR	4 cut(s)	922, 1344, 2247, 2439
AcsI	RAATTY	8 cut(s)	448, 1399, 1443, 1661, 1682, 2533, 2876, 3771
AcvI	CACGTG	1 cut(s)	2297
AcyI	GRCGYC	1 cut(s)	3753
AdeI	CACNNNGTG	1 cut(s)	1370
AfaI	GTAC	3 cut(s)	243, 327, 2397
AfiI	CCNNNNNNNGG	3 cut(s)	778, 1087, 2256
AflII	CTTAAG	1 cut(s)	2144
AflIII	ACRYGT	1 cut(s)	953
AjnI	CCWGG	5 cut(s)	108, 2329, 2521, 2583, 2723
AloI	GAACNNNNNNTCC	2 cut(s)	1197, 1229
Alw21I	GWGCWC	6 cut(s)	641, 1072, 1195, 1698, 1894, 1956
Alw26I	GTCTC	8 cut(s)	356, 1031, 1498, 1843, 1984, 2523, 2673, 3446
Alw44I	GTGCAC	1 cut(s)	1191
AlwI	GGATC	3 cut(s)	622, 1100, 1207
AlwNI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3692
Aor13HI	TCCGGA	1 cut(s)	161
AoxI	GGCC	7 cut(s)	41, 922, 1344, 2247, 2439, 3626, 3675
ApaLI	GTGCAC	1 cut(s)	1191
ApoI	RAATTY	8 cut(s)	448, 1399, 1443, 1661, 1682, 2533, 2876, 3771
Asp700I	GAANNNNTTC	5 cut(s)	18, 843, 2136, 2287, 2850
AspLEI	GCGC	6 cut(s)	764, 2625, 2940, 3275, 3319, 3755
AspS9I	GGNCC	4 cut(s)	42, 2327, 2905, 3675
AsuC2I	CCSGG	2 cut(s)	99, 2954
AsuHPI	GGTGA	5 cut(s)	113, 758, 3037, 3155, 3479
AvaII	GGWCC	2 cut(s)	2327, 2905
BaeGI	GKGCMC	2 cut(s)	1195, 2650
BaeI	ACNNNNGTAYC	2 cut(s)	1752, 1785
BalI	TGGCCA	3 cut(s)	1346, 2249, 2441
BanI	GGYRCC	2 cut(s)	3752, 3781
BanII	GRGCYC	2 cut(s)	1698, 1956
BarI	GAAGNNNNNNTAC	2 cut(s)	1899, 1931
BauI	CACGAG	4 cut(s)	609, 624, 1265, 2121
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	2 cut(s)	2234, 3231
Bbv12I	GWGCWC	6 cut(s)	641, 1072, 1195, 1698, 1894, 1956
BccI	CCATC	7 cut(s)	331, 586, 1880, 2431, 3008, 3052, 3458
BceAI	ACGGC	1 cut(s)	648
BcgI	CGANNNNNNTGC	8 cut(s)	73, 107, 179, 213, 1172, 1206, 2517, 2551
BciT130I	CCWGG	5 cut(s)	110, 2331, 2523, 2585, 2725
BciVI	GTATCC	2 cut(s)	221, 2974
BclI	TGATCA	1 cut(s)	390
BcnI	CCSGG	2 cut(s)	99, 2954
BcoDI	GTCTC	8 cut(s)	356, 1031, 1498, 1843, 1984, 2523, 2673, 3446
BfaI	CTAG	5 cut(s)	1188, 2433, 2577, 3219, 3447
BfmI	CTRYAG	1 cut(s)	1826
BfoI	RGCGCY	1 cut(s)	3756
BfrI	CTTAAG	1 cut(s)	2144
BfuAI	ACCTGC	2 cut(s)	493, 1933
BfuI	GTATCC	2 cut(s)	221, 2974
Bme1390I	CCNGG	7 cut(s)	99, 110, 2331, 2523, 2585, 2725, 2954
Bme18I	GGWCC	2 cut(s)	2327, 2905
BmgT120I	GGNCC	4 cut(s)	42, 2327, 2905, 3675
BmiI	GGNNCC	4 cut(s)	265, 2657, 3754, 3783
BmrFI	CCNGG	7 cut(s)	99, 110, 2331, 2523, 2585, 2725, 2954
BmrI	ACTGGG	3 cut(s)	642, 2649, 3031
BmuI	ACTGGG	3 cut(s)	642, 2649, 3031
BpiI	GAAGAC	2 cut(s)	2234, 3231
BplI	GAGNNNNNCTC	4 cut(s)	694, 726, 1379, 1411
BpmI	CTGGAG	3 cut(s)	321, 2352, 2707
Bpu10I	CCTNAGC	1 cut(s)	1323
BpuEI	CTTGAG	3 cut(s)	1959, 2008, 2827
BpuMI	CCSGG	2 cut(s)	99, 2954
BsaAI	YACGTR	1 cut(s)	2297
BsaBI	GATNNNNATC	1 cut(s)	619
BsaHI	GRCGYC	1 cut(s)	3753
BsaI	GGTCTC	1 cut(s)	2673
BsaWI	WCCGGW	2 cut(s)	161, 266
BsaXI	ACNNNNNCTCC	2 cut(s)	3568, 3598
Bsc4I	CCNNNNNNNGG	3 cut(s)	778, 1087, 2256
Bse118I	RCCGGY	1 cut(s)	3234
Bse1I	ACTGG	7 cut(s)	304, 637, 655, 1347, 2644, 2949, 3026
Bse3DI	GCAATG	2 cut(s)	883, 1255
Bse8I	GATNNNNATC	1 cut(s)	619
BseAI	TCCGGA	1 cut(s)	161
BseBI	CCWGG	5 cut(s)	110, 2331, 2523, 2585, 2725
BseGI	GGATG	7 cut(s)	1077, 1390, 2038, 2442, 2905, 3019, 3654
BseJI	GATNNNNATC	1 cut(s)	619
BseLI	CCNNNNNNNGG	3 cut(s)	778, 1087, 2256
BseMI	GCAATG	2 cut(s)	883, 1255
BseMII	CTCAG	8 cut(s)	555, 786, 1133, 1337, 1512, 1941, 2085, 2394
BseNI	ACTGG	7 cut(s)	304, 637, 655, 1347, 2644, 2949, 3026
BseRI	GAGGAG	3 cut(s)	1856, 2173, 3196
BseSI	GKGCMC	2 cut(s)	1195, 2650
BseYI	CCCAGC	2 cut(s)	499, 1604
BsgI	GTGCAG	2 cut(s)	58, 3312
Bsh1236I	CGCG	4 cut(s)	2281, 2625, 3275, 3335
BshFI	GGCC	7 cut(s)	43, 924, 1346, 2249, 2441, 3628, 3677
BshNI	GGYRCC	2 cut(s)	3752, 3781
BsiHKAI	GWGCWC	6 cut(s)	641, 1072, 1195, 1698, 1894, 1956
BsiSI	CCGG	6 cut(s)	99, 162, 267, 2953, 3235, 3799
BslFI	GGGAC	4 cut(s)	136, 301, 482, 3382
BslI	CCNNNNNNNGG	3 cut(s)	778, 1087, 2256
BsmAI	GTCTC	8 cut(s)	356, 1031, 1498, 1843, 1984, 2523, 2673, 3446
BsmBI	CGTCTC	1 cut(s)	1031
BsmFI	GGGAC	4 cut(s)	136, 301, 482, 3382
BsmI	GAATGC	6 cut(s)	831, 843, 2866, 3236, 3433, 3733
BsnI	GGCC	7 cut(s)	43, 924, 1346, 2249, 2441, 3628, 3677
Bso31I	GGTCTC	1 cut(s)	2673
Bsp1286I	GDGCHC	7 cut(s)	641, 1072, 1195, 1698, 1894, 1956, 2650
Bsp13I	TCCGGA	1 cut(s)	161
Bsp143I	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
Bsp19I	CCATGG	2 cut(s)	223, 1110
Bsp68I	TCGCGA	1 cut(s)	2281
BspANI	GGCC	7 cut(s)	43, 924, 1346, 2249, 2441, 3628, 3677
BspCNI	CTCAG	8 cut(s)	556, 787, 1132, 1336, 1513, 1942, 2086, 2395
BspEI	TCCGGA	1 cut(s)	161
BspFNI	CGCG	4 cut(s)	2281, 2625, 3275, 3335
BspLI	GGNNCC	4 cut(s)	265, 2657, 3754, 3783
BspMAI	CTGCAG	1 cut(s)	1830
BspMI	ACCTGC	2 cut(s)	493, 1933
BspPI	GGATC	3 cut(s)	622, 1100, 1207
BspQI	GCTCTTC	2 cut(s)	2418, 2613
BspT107I	GGYRCC	2 cut(s)	3752, 3781
BspTI	CTTAAG	1 cut(s)	2144
BspTNI	GGTCTC	1 cut(s)	2673
BsrBI	CCGCTC	3 cut(s)	799, 2428, 2629
BsrDI	GCAATG	2 cut(s)	883, 1255
BsrFI	RCCGGY	1 cut(s)	3234
BsrI	ACTGG	7 cut(s)	304, 637, 655, 1347, 2644, 2949, 3026
BssAI	RCCGGY	1 cut(s)	3234
BssMI	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
BssNI	GRCGYC	1 cut(s)	3753
BssSI	CACGAG	4 cut(s)	609, 624, 1265, 2121
BssT1I	CCWWGG	7 cut(s)	223, 891, 1110, 1379, 2442, 2895, 3671
Bst2BI	CACGAG	4 cut(s)	609, 624, 1265, 2121
Bst2UI	CCWGG	5 cut(s)	110, 2331, 2523, 2585, 2725
Bst4CI	ACNGT	6 cut(s)	241, 532, 994, 1152, 2380, 2891
Bst6I	CTCTTC	8 cut(s)	582, 695, 1029, 1677, 2418, 2613, 3375, 3800
BstACI	GRCGYC	1 cut(s)	3753
BstAFI	CTTAAG	1 cut(s)	2144
BstAPI	GCANNNNNTGC	2 cut(s)	81, 485
BstBAI	YACGTR	1 cut(s)	2297
BstC8I	GCNNGC	9 cut(s)	801, 1072, 2314, 2352, 2430, 2565, 2627, 2650, 3652
BstDSI	CCRYGG	2 cut(s)	223, 1110
BstF5I	GGATG	7 cut(s)	1077, 1390, 2038, 2442, 2905, 3019, 3654
BstFNI	CGCG	4 cut(s)	2281, 2625, 3275, 3335
BstH2I	RGCGCY	1 cut(s)	3756
BstHHI	GCGC	6 cut(s)	764, 2625, 2940, 3275, 3319, 3755
BstKTI	GATC	7 cut(s)	393, 617, 1108, 1215, 1636, 2082, 3686
BstMAI	GTCTC	8 cut(s)	356, 1031, 1498, 1843, 1984, 2523, 2673, 3446
BstMBI	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
BstNI	CCWGG	5 cut(s)	110, 2331, 2523, 2585, 2725
BstNSI	RCATGY	2 cut(s)	674, 2563
BstSCI	CCNGG	7 cut(s)	97, 108, 2329, 2521, 2583, 2723, 2952
BstSFI	CTRYAG	1 cut(s)	1826
BstSLI	GKGCMC	2 cut(s)	1195, 2650
BstUI	CGCG	4 cut(s)	2281, 2625, 3275, 3335
BstV2I	GAAGAC	2 cut(s)	2234, 3231
BstX2I	RGATCY	1 cut(s)	1212
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	1 cut(s)	1212
BsuI	GTATCC	2 cut(s)	221, 2974
BsuRI	GGCC	7 cut(s)	43, 924, 1346, 2249, 2441, 3628, 3677
BtgI	CCRYGG	2 cut(s)	223, 1110
BtgZI	GCGATG	1 cut(s)	1197
BtsCI	GGATG	7 cut(s)	1077, 1390, 2038, 2442, 2905, 3019, 3654
BtsI	GCAGTG	2 cut(s)	1823, 2323
BtsIMutI	CAGTG	5 cut(s)	537, 561, 999, 1823, 2323
BtuMI	TCGCGA	1 cut(s)	2281
BveI	ACCTGC	2 cut(s)	493, 1933
Cac8I	GCNNGC	9 cut(s)	801, 1072, 2314, 2352, 2430, 2565, 2627, 2650, 3652
CaiI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3692
CfoI	GCGC	6 cut(s)	764, 2625, 2940, 3275, 3319, 3755
Cfr10I	RCCGGY	1 cut(s)	3234
Cfr13I	GGNCC	4 cut(s)	42, 2327, 2905, 3675
CseI	GACGC	2 cut(s)	1250, 2402
Csp6I	GTAC	3 cut(s)	242, 326, 2396
CviQI	GTAC	3 cut(s)	242, 326, 2396
DinI	GGCGCC	1 cut(s)	3754
DpnI	GATC	7 cut(s)	392, 616, 1107, 1214, 1635, 2081, 3685
DpnII	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
DraIII	CACNNNGTG	1 cut(s)	1370
EaeI	YGGCCR	4 cut(s)	922, 1344, 2247, 2439
Eam1104I	CTCTTC	8 cut(s)	582, 695, 1029, 1677, 2418, 2613, 3375, 3800
EarI	CTCTTC	8 cut(s)	582, 695, 1029, 1677, 2418, 2613, 3375, 3800
EciI	GGCGGA	1 cut(s)	3576
Ecl136II	GAGCTC	2 cut(s)	1696, 1954
Eco130I	CCWWGG	7 cut(s)	223, 891, 1110, 1379, 2442, 2895, 3671
Eco24I	GRGCYC	2 cut(s)	1698, 1956
Eco31I	GGTCTC	1 cut(s)	2673
Eco32I	GATATC	1 cut(s)	754
Eco47I	GGWCC	2 cut(s)	2327, 2905
Eco53kI	GAGCTC	2 cut(s)	1696, 1954
Eco72I	CACGTG	1 cut(s)	2297
EcoICRI	GAGCTC	2 cut(s)	1696, 1954
EcoO109I	RGGNCCY	1 cut(s)	42
EcoRI	GAATTC	2 cut(s)	1399, 3771
EcoRII	CCWGG	5 cut(s)	108, 2329, 2521, 2583, 2723
EcoRV	GATATC	1 cut(s)	754
EcoT14I	CCWWGG	7 cut(s)	223, 891, 1110, 1379, 2442, 2895, 3671
EcoT22I	ATGCAT	2 cut(s)	2181, 3433
EcoT38I	GRGCYC	2 cut(s)	1698, 1956
EgeI	GGCGCC	1 cut(s)	3754
EheI	GGCGCC	1 cut(s)	3754
ErhI	CCWWGG	7 cut(s)	223, 891, 1110, 1379, 2442, 2895, 3671
Esp3I	CGTCTC	1 cut(s)	1031
FalI	AAGNNNNNCTT	2 cut(s)	2121, 2153
FaqI	GGGAC	4 cut(s)	136, 301, 482, 3382
FauI	CCCGC	4 cut(s)	792, 2421, 2622, 3603
FauNDI	CATATG	2 cut(s)	139, 1198
FbaI	TGATCA	1 cut(s)	390
FokI	GGATG	7 cut(s)	1064, 1397, 2045, 2449, 2912, 3026, 3641
FriOI	GRGCYC	2 cut(s)	1698, 1956
FspBI	CTAG	5 cut(s)	1188, 2433, 2577, 3219, 3447
FspI	TGCGCA	1 cut(s)	2939
GlaI	GCGC	6 cut(s)	763, 2624, 2939, 3274, 3318, 3754
GsaI	CCCAGC	2 cut(s)	503, 1608
GsuI	CTGGAG	3 cut(s)	321, 2352, 2707
HaeII	RGCGCY	1 cut(s)	3756
HaeIII	GGCC	7 cut(s)	43, 924, 1346, 2249, 2441, 3628, 3677
HapII	CCGG	6 cut(s)	99, 162, 267, 2953, 3235, 3799
HgaI	GACGC	2 cut(s)	1250, 2402
HhaI	GCGC	6 cut(s)	764, 2625, 2940, 3275, 3319, 3755
Hin1I	GRCGYC	1 cut(s)	3753
Hin6I	GCGC	6 cut(s)	762, 2623, 2938, 3273, 3317, 3753
HinP1I	GCGC	6 cut(s)	762, 2623, 2938, 3273, 3317, 3753
HincII	GTYRAC	1 cut(s)	1621
HindII	GTYRAC	1 cut(s)	1621
HindIII	AAGCTT	2 cut(s)	2238, 2803
HpaII	CCGG	6 cut(s)	99, 162, 267, 2953, 3235, 3799
HphI	GGTGA	5 cut(s)	113, 758, 3037, 3155, 3479
Hpy99I	CGWCG	2 cut(s)	95, 1284
HpyCH4III	ACNGT	6 cut(s)	241, 532, 994, 1152, 2380, 2891
HpyCH4IV	ACGT	9 cut(s)	955, 1040, 1279, 1309, 2296, 2398, 2984, 3506, 3702
HpySE526I	ACGT	9 cut(s)	955, 1040, 1279, 1309, 2296, 2398, 2984, 3506, 3702
Hsp92I	GRCGYC	1 cut(s)	3753
HspAI	GCGC	6 cut(s)	762, 2623, 2938, 3273, 3317, 3753
KasI	GGCGCC	1 cut(s)	3752
Kpn2I	TCCGGA	1 cut(s)	161
Ksp22I	TGATCA	1 cut(s)	390
Kzo9I	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
LguI	GCTCTTC	2 cut(s)	2418, 2613
MaeI	CTAG	5 cut(s)	1188, 2433, 2577, 3219, 3447
MaeII	ACGT	9 cut(s)	955, 1040, 1279, 1309, 2296, 2398, 2984, 3506, 3702
MaeIII	GTNAC	8 cut(s)	559, 960, 1152, 1233, 1262, 2374, 3400, 3574
MalI	GATC	7 cut(s)	392, 616, 1107, 1214, 1635, 2081, 3685
MbiI	CCGCTC	3 cut(s)	799, 2428, 2629
MboI	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
MfeI	CAATTG	1 cut(s)	2355
MflI	RGATCY	1 cut(s)	1212
MhlI	GDGCHC	7 cut(s)	641, 1072, 1195, 1698, 1894, 1956, 2650
MlsI	TGGCCA	3 cut(s)	1346, 2249, 2441
MluNI	TGGCCA	3 cut(s)	1346, 2249, 2441
Mly113I	GGCGCC	1 cut(s)	3753
MlyI	GAGTC	6 cut(s)	1388, 1532, 2047, 2099, 2818, 3107
MmeI	TCCRAC	2 cut(s)	3097, 3543
Mox20I	TGGCCA	3 cut(s)	1346, 2249, 2441
Mph1103I	ATGCAT	2 cut(s)	2181, 3433
MroI	TCCGGA	1 cut(s)	161
MroXI	GAANNNNTTC	5 cut(s)	18, 843, 2136, 2287, 2850
MscI	TGGCCA	3 cut(s)	1346, 2249, 2441
MseI	TTAA	7 cut(s)	351, 417, 950, 1773, 2145, 2480, 3108
MslI	CAYNNNNRTG	4 cut(s)	644, 2859, 2928, 3659
Msp20I	TGGCCA	3 cut(s)	1346, 2249, 2441
MspA1I	CMGCKG	1 cut(s)	1019
MspCI	CTTAAG	1 cut(s)	2144
MspI	CCGG	6 cut(s)	99, 162, 267, 2953, 3235, 3799
MspR9I	CCNGG	7 cut(s)	99, 110, 2331, 2523, 2585, 2725, 2954
MunI	CAATTG	1 cut(s)	2355
Mva1269I	GAATGC	6 cut(s)	831, 843, 2866, 3236, 3433, 3733
MvaI	CCWGG	5 cut(s)	110, 2331, 2523, 2585, 2725
MvnI	CGCG	4 cut(s)	2281, 2625, 3275, 3335
NarI	GGCGCC	1 cut(s)	3753
NciI	CCSGG	2 cut(s)	99, 2954
NcoI	CCATGG	2 cut(s)	223, 1110
NdeI	CATATG	2 cut(s)	139, 1198
NdeII	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
NlaIV	GGNNCC	4 cut(s)	265, 2657, 3754, 3783
NmuCI	GTSAC	3 cut(s)	559, 1262, 2374
NruI	TCGCGA	1 cut(s)	2281
NsbI	TGCGCA	1 cut(s)	2939
NsiI	ATGCAT	2 cut(s)	2181, 3433
NspI	RCATGY	2 cut(s)	674, 2563
PaqCI	CACCTGC	1 cut(s)	493
PciSI	GCTCTTC	2 cut(s)	2418, 2613
PctI	GAATGC	6 cut(s)	831, 843, 2866, 3236, 3433, 3733
PdmI	GAANNNNTTC	5 cut(s)	18, 843, 2136, 2287, 2850
PflFI	GACNNNGTC	1 cut(s)	2325
PflMI	CCANNNNNTGG	1 cut(s)	778
PfoI	TCCNGGA	1 cut(s)	2329
PleI	GAGTC	6 cut(s)	1388, 1531, 2047, 2098, 2817, 3106
PluTI	GGCGCC	1 cut(s)	3756
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	6 cut(s)	1388, 1531, 2047, 2098, 2817, 3106
Ppu21I	YACGTR	1 cut(s)	2297
Psp124BI	GAGCTC	2 cut(s)	1698, 1956
Psp1406I	AACGTT	1 cut(s)	3702
Psp6I	CCWGG	5 cut(s)	108, 2329, 2521, 2583, 2723
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	2 cut(s)	499, 1604
PspGI	CCWGG	5 cut(s)	108, 2329, 2521, 2583, 2723
PspN4I	GGNNCC	4 cut(s)	265, 2657, 3754, 3783
PspPI	GGNCC	4 cut(s)	42, 2327, 2905, 3675
PstI	CTGCAG	1 cut(s)	1830
PstNI	CAGNNNCTG	5 cut(s)	485, 794, 818, 1216, 3692
PsuI	RGATCY	1 cut(s)	1212
PsyI	GACNNNGTC	1 cut(s)	2325
PvuII	CAGCTG	1 cut(s)	1019
RruI	TCGCGA	1 cut(s)	2281
RsaI	GTAC	3 cut(s)	243, 327, 2397
RsaNI	GTAC	3 cut(s)	242, 326, 2396
RseI	CAYNNNNRTG	4 cut(s)	644, 2859, 2928, 3659
SacI	GAGCTC	2 cut(s)	1698, 1956
SapI	GCTCTTC	2 cut(s)	2418, 2613
SaqAI	TTAA	7 cut(s)	351, 417, 950, 1773, 2145, 2480, 3108
Sau3AI	GATC	7 cut(s)	390, 614, 1105, 1212, 1633, 2079, 3683
Sau96I	GGNCC	4 cut(s)	42, 2327, 2905, 3675
SchI	GAGTC	6 cut(s)	1388, 1532, 2047, 2099, 2818, 3107
ScrFI	CCNGG	7 cut(s)	99, 110, 2331, 2523, 2585, 2725, 2954
SduI	GDGCHC	7 cut(s)	641, 1072, 1195, 1698, 1894, 1956, 2650
SfcI	CTRYAG	1 cut(s)	1826
SfoI	GGCGCC	1 cut(s)	3754
SinI	GGWCC	2 cut(s)	2327, 2905
SmiMI	CAYNNNNRTG	4 cut(s)	644, 2859, 2928, 3659
SmlI	CTYRAG	4 cut(s)	1974, 1987, 2144, 2806
SmoI	CTYRAG	4 cut(s)	1974, 1987, 2144, 2806
SspDI	GGCGCC	1 cut(s)	3752
SspI	AATATT	2 cut(s)	678, 1580
SspMI	CTAG	5 cut(s)	1188, 2433, 2577, 3219, 3447
SstI	GAGCTC	2 cut(s)	1698, 1956
StyD4I	CCNGG	7 cut(s)	97, 108, 2329, 2521, 2583, 2723, 2952
StyI	CCWWGG	7 cut(s)	223, 891, 1110, 1379, 2442, 2895, 3671
TaaI	ACNGT	6 cut(s)	241, 532, 994, 1152, 2380, 2891
TaiI	ACGT	9 cut(s)	958, 1043, 1282, 1312, 2299, 2401, 2987, 3509, 3705
TaqI	TCGA	5 cut(s)	1239, 1282, 1397, 2537, 3622
TaqII	GACCGA	1 cut(s)	2655
TatI	WGTACW	1 cut(s)	241
TauI	GCSGC	3 cut(s)	924, 3278, 3782
Tru1I	TTAA	7 cut(s)	351, 417, 950, 1773, 2145, 2480, 3108
Tru9I	TTAA	7 cut(s)	351, 417, 950, 1773, 2145, 2480, 3108
TscAI	CASTG	5 cut(s)	537, 568, 999, 1830, 2323
TseFI	GTSAC	3 cut(s)	559, 1262, 2374
Tsp45I	GTSAC	3 cut(s)	559, 1262, 2374
TspGWI	ACGGA	5 cut(s)	79, 395, 823, 837, 2239
TspRI	CASTG	5 cut(s)	537, 568, 999, 1830, 2323
Tth111I	GACNNNGTC	1 cut(s)	2325
Van91I	CCANNNNNTGG	1 cut(s)	778
Vha464I	CTTAAG	1 cut(s)	2144
VneI	GTGCAC	1 cut(s)	1191
VpaK11BI	GGWCC	2 cut(s)	2327, 2905
XapI	RAATTY	8 cut(s)	448, 1399, 1443, 1661, 1682, 2533, 2876, 3771
XceI	RCATGY	2 cut(s)	674, 2563
XcmI	CCANNNNNNNNNTGG	1 cut(s)	116
XmnI	GAANNNNTTC	5 cut(s)	18, 843, 2136, 2287, 2850
XspI	CTAG	5 cut(s)	1188, 2433, 2577, 3219, 3447
Zsp2I	ATGCAT	2 cut(s)	2181, 3433

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.