Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003716

Physical Location & Seq

Genomic Coordinates Reverse (-)

26846234 .. 26851515

Gene Structure 5282 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1206700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGACACCCCAGCCACCGCCTTAGAAGTGGAGGCCGCTGCTATAACCGAGATCGAGGAGGACAAGAACGACGATGACGTGTCATCGGTCGAGCAGGTCCGTCTAACGGTCTCCAACGAAGACGACAGTTCCCTCCCTGTCTGGACGTTCCGTATGTGGACTATAGGCATATGCTCGTGCTTCCTCCTCTCATTCCTCAACACCTTCTTCTCGTACCGCACGGAGCCACTCGTGATATCAATGATTTCCGTGCAGGTGGCAAGTCTGCCGATCGGGAGGTTCATGGCTAGGACGCTGCCGAGGAGAAAGTTTTGGATTGGTGGGAAGTCATTCTCGTTGAACCCGGGAGAATTTAACATAAAAGAGCACGTGCTGATTTCGATATTTGCAAACGCGGGATCTGGGTTTGGGAACGGACCGGCGTATGCGGTTGGTATTGTGAATATAATCAAAGCGTTTTACGGTAGGAAGATATCCTTCTTTGCAAGTTGGGCACTTGTGATTACAACACAGGTATTGGGGTATGGGTGGGCTGGTATTATCAGAAAGTACGTGGTGGATCCGGCGGCGATGTGGTGGCCTAGTAGTCTCGTTCAGGTTTCTTTGTTCAGGGCTCTGCATGAGAAAGACAAAAATCGAATGTCCAGAGGGAAATTCTTTTTGATAGCACTGGTATGCAGCTTCTCTTGGTACGCAGTGCCGGGCTACCTCTTCACAACTCTCTCAATAATTTCATGGATTTGTTGGATTTACCCTAAGTCCATAAGAGCTCAGCAAATCGGCTCAGGCTACCGTGGACTTGGGCTTGGGTCATTTACCCTAGATTGGTCGGTCATATCCTCTTACTTGGGGAGCCCTTTGATCAGCCCCTTCTTTGCCATTGCCAACATCACCGTCGGATACATCGCCATCATGTATGTCATCATTCCGATTGCCTACTGGAGGCTCAACTTGTACAATGCAAAGAATTTTGCAATCTTCTCTTCTCACTTGTTCGATTCCGATGGCCAAATATATAATGTCAAAGGAATTGTGAATGACCAGTTTCAGATTGACATGCCTGCCTATGAAAAACAAGGATACATAAACTTGAGTGTATTCTTTACCCTATCATATGGTCTTGGTTTTGCTGCTGTTATATCAACCCTCACACATGTGGCTCTATTCAATGGAAGGGACATATATGAACGATATAAATCGTCATATAGTGGAAAGATAGATATACACACCAAATTAATGAGGAAATACAGGGACATACCCAGCTGGTGGTTTCATGTTTTGCTTGTAGTGTCGTTCGTATTCTCTCTAGCATTGTGCATCTTCATGAAGGATGAGGTGCAAATGCCATGGTGGGGACTCATTTTTGCAGCAGGAATTGCATTGATTTTCACACTTCCAATCAGCATAATAACTGCCACAACAAATGTTACCCCGGGACTAAACATCATCACGGAGTACGTCATGGGTTTGATACTACCTGGATATCCGATAGCCAATGTATGCTTCAAAACTTATGGATACATAAGTATGTCACAGGCAATTTCATTTTTGAGTGATTTTAAGTTAGGCCATTACATGAAGATTCCTCCCATATCCATGTTCGTAGTTCAGGGAAAGTTTCTAATTTGGGACGCATGGGAAGCTGCTATCTTCTTATTACTCTGGGTTTGTAATTTTCTTTTTTCTGCCATTATAAAGTGCATAGGAACTATAGTAGCCGGAACAGTCAATATTGGGACAGCATGGTGGCTGCTAACTACAGTGGAAAACATATGCAATACTGACCTACTCCCGGACACTAGTCCTTGGACTTGTCCTAATGATAGTGTCTTCTTCACTGCATCTGTGATTTGGGGTCTGGTTGGACCGACACGAATCTTTGGCTCTCTCGGACTCTACGGAGGACTCAACTGGTTCTTCGTGGTAGGTGCACTCGGACCTGTCTTGGTATGGTTGTTGCACAAAGCTTTCCCTAGTCAGAAATGGATCGCCTACATCAACCTTCCAGTGCTTTTCGGAGCAACGGCTGCTATGCCACCAGCTACAACTGTGAACTTCAACAGCTGGATTGTGGTCGGAATCGTTTTCAATTACTTTGTTTTCAGATACAGAAAGAAGTGGTGGCAGAGGTACAATTATGTTCTTTCTGCTGCCCTAGATGCTGGAGTGGCTTTCATGGGGGTGCTTATATATATATGTTTAACCATAAACAACGTAAGCATTTCCTGGTGGGGTACAGCGGACTATGACCACTGTATATTGTCTACTTGTCCAACATCCAAGGGCATAGAACTCGATCATGCTTGTGTATTCCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006857 GO:0008150 GO:0015833 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0035672 GO:0035673 GO:0042886 GO:0042887 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:1904680

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OPT

Protein Analysis

773

Amino Acids

86.68

Weight (kDa)

8.57

Isoelectric Point (pI)

26.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OPT	PF03169	47 - 733	6.1e-175	OPT oligopeptide transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09930
fragaria_vesca	FvH4_5g34010 FvH4_5g34011
malus_domestica	MD08G1206700.v1.1 MD15G1390200.v1.1 MD15G1390400.v1.1
prunus_persica	Prupe.1G539200_v2.0.a1 Prupe.1G539300_v2.0.a1
pyrus_communis	pycom15g34950 pycom15g34960
rosa_chinensis	RchiOBHm_Chr7g0233451 RchiOBHm_Chr7g0233471
rosa_laevigata	RLG00000001268 RLG00000001269
rosa_multiflora	Rmu_sc0000029.1_g000038 Rmu_sc0000029.1_g000051
rosa_roxburghii	Rroxscaffold_3G00227270 Rroxscaffold_3G00227290
rosa_rugosa	Rorug07G0280800 Rorug07G0280800
rosa_samantha	Rh7AG436600 Rh7AG436700 Rh7BG409700 Rh7BG409800 Rh7CG456500 Rh7CG456700 Rh7DG425800 Rh7DG425900
rosa_wichuraiana	Rw0G008530 Rw0G022500 Rw7G036000 Rw7G036010 Rw7G036030 Rw7G036040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1694
AarI	CACCTGC	1 cut(s)	244
Acc36I	ACCTGC	2 cut(s)	85, 244
AccB7I	CCANNNNNTGG	1 cut(s)	1266
AccI	GTMKAC	1 cut(s)	2264
AccII	CGCG	1 cut(s)	395
AciI	CCGC	7 cut(s)	18, 36, 217, 395, 428, 566, 2240
AclWI	GGATC	4 cut(s)	406, 554, 567, 1994
AcoI	YGGCCR	1 cut(s)	1006
AcsI	RAATTY	3 cut(s)	350, 653, 967
AcvI	CACGTG	1 cut(s)	370
AfaI	GTAC	7 cut(s)	215, 551, 692, 956, 1457, 2132, 2236
AfiI	CCNNNNNNNGG	6 cut(s)	106, 825, 942, 1266, 1352, 1792
AflIII	ACRYGT	2 cut(s)	78, 1153
AgsI	TTSAA	5 cut(s)	340, 1168, 1507, 2059, 2089
AhlI	ACTAGT	1 cut(s)	1799
AjiI	CACGTC	1 cut(s)	79
AjnI	CCWGG	2 cut(s)	1477, 2225
AjuI	GAANNNNNNNTTGG	2 cut(s)	1487, 1519
AleI	CACNNNNGTG	1 cut(s)	1154
AluBI	AGCT	7 cut(s)	681, 770, 1263, 1643, 1967, 2042, 2064
AluI	AGCT	7 cut(s)	681, 770, 1263, 1643, 1967, 2042, 2064
Alw21I	GWGCWC	3 cut(s)	369, 772, 1933
Alw26I	GTCTC	2 cut(s)	115, 593
Alw44I	GTGCAC	1 cut(s)	1929
AlwI	GGATC	4 cut(s)	406, 554, 567, 1994
Ama87I	CYCGRG	2 cut(s)	343, 1432
AoxI	GGCC	4 cut(s)	33, 578, 1006, 1567
ApaLI	GTGCAC	1 cut(s)	1929
ApeKI	GCWGC	9 cut(s)	38, 295, 678, 1130, 1367, 1643, 1750, 2027, 2150
ApoI	RAATTY	3 cut(s)	350, 653, 967
AseI	ATTAAT	1 cut(s)	1235
Asp700I	GAANNNNTTC	1 cut(s)	329
AspS9I	GGNCC	4 cut(s)	97, 416, 1865, 1937
AsuC2I	CCSGG	6 cut(s)	344, 345, 702, 1433, 1434, 1793
AsuHPI	GGTGA	1 cut(s)	883
AvaI	CYCGRG	2 cut(s)	343, 1432
AvaII	GGWCC	4 cut(s)	97, 416, 1865, 1937
BaeGI	GKGCMC	2 cut(s)	496, 1933
BaeI	ACNNNNGTAYC	2 cut(s)	1510, 1543
BalI	TGGCCA	1 cut(s)	1008
BamHI	GGATCC	1 cut(s)	559
BanII	GRGCYC	3 cut(s)	616, 772, 857
BauI	CACGAG	2 cut(s)	175, 230
BbrPI	CACGTG	1 cut(s)	370
BbsI	GAAGAC	2 cut(s)	126, 1822
Bbv12I	GWGCWC	3 cut(s)	369, 772, 1933
BbvI	GCAGC	9 cut(s)	25, 282, 690, 1117, 1379, 1630, 1737, 2014, 2137
BccI	CCATC	2 cut(s)	917, 998
BceAI	ACGGC	1 cut(s)	2040
BcgI	CGANNNNNNTGC	2 cut(s)	2275, 2309
BciT130I	CCWGG	2 cut(s)	1479, 2227
BciVI	GTATCC	3 cut(s)	893, 1073, 1511
BclI	TGATCA	1 cut(s)	861
BcnI	CCSGG	6 cut(s)	344, 345, 702, 1433, 1434, 1793
BcoDI	GTCTC	2 cut(s)	115, 593
BcuI	ACTAGT	1 cut(s)	1799
BfaI	CTAG	7 cut(s)	288, 582, 821, 1307, 1800, 1974, 2156
BfmI	CTRYAG	3 cut(s)	162, 1710, 1758
BfuAI	ACCTGC	2 cut(s)	85, 244
BfuI	GTATCC	3 cut(s)	893, 1073, 1511
BlpI	GCTNAGC	1 cut(s)	771
Bme1390I	CCNGG	8 cut(s)	344, 345, 702, 1433, 1434, 1479, 1793, 2227
Bme18I	GGWCC	4 cut(s)	97, 416, 1865, 1937
BmeT110I	CYCGRG	2 cut(s)	343, 1432
BmgBI	CACGTC	1 cut(s)	79
BmgT120I	GGNCC	4 cut(s)	97, 416, 1865, 1937
BmiI	GGNNCC	3 cut(s)	225, 561, 854
BmrFI	CCNGG	8 cut(s)	344, 345, 702, 1433, 1434, 1479, 1793, 2227
BmsI	GCATC	3 cut(s)	1326, 1850, 2149
BoxI	GACNNNNGTC	1 cut(s)	1800
BpiI	GAAGAC	2 cut(s)	126, 1822
BpmI	CTGGAG	2 cut(s)	961, 2184
Bpu10I	CCTNAGC	1 cut(s)	784
Bpu1102I	GCTNAGC	1 cut(s)	771
BpuEI	CTTGAG	1 cut(s)	1111
BpuMI	CCSGG	6 cut(s)	344, 345, 702, 1433, 1434, 1793
BsaAI	YACGTR	2 cut(s)	370, 553
BsaBI	GATNNNNATC	1 cut(s)	1195
BsaI	GGTCTC	1 cut(s)	115
BsaJI	CCNNGG	8 cut(s)	299, 343, 793, 1346, 1431, 1432, 1805, 2280
Bsc4I	CCNNNNNNNGG	6 cut(s)	106, 825, 942, 1266, 1352, 1792
Bse118I	RCCGGY	1 cut(s)	418
Bse1I	ACTGG	5 cut(s)	675, 944, 1042, 1916, 2006
Bse3DI	GCAATG	1 cut(s)	879
Bse8I	GATNNNNATC	1 cut(s)	1195
BseBI	CCWGG	2 cut(s)	1479, 2227
BseDI	CCNNGG	8 cut(s)	299, 343, 793, 1346, 1431, 1432, 1805, 2280
BseGI	GGATG	2 cut(s)	1336, 2276
BseJI	GATNNNNATC	1 cut(s)	1195
BseLI	CCNNNNNNNGG	6 cut(s)	106, 825, 942, 1266, 1352, 1792
BseMI	GCAATG	1 cut(s)	879
BseMII	CTCAG	2 cut(s)	785, 798
BseNI	ACTGG	5 cut(s)	675, 944, 1042, 1916, 2006
BseRI	GAGGAG	3 cut(s)	71, 176, 316
BseSI	GKGCMC	2 cut(s)	496, 1933
BseXI	GCAGC	9 cut(s)	25, 282, 690, 1117, 1379, 1630, 1737, 2014, 2137
BseYI	CCCAGC	2 cut(s)	10, 1259
BsgI	GTGCAG	1 cut(s)	272
Bsh1236I	CGCG	1 cut(s)	395
Bsh1285I	CGRYCG	2 cut(s)	90, 273
BshFI	GGCC	4 cut(s)	35, 580, 1008, 1569
BsiEI	CGRYCG	2 cut(s)	90, 273
BsiHKAI	GWGCWC	3 cut(s)	369, 772, 1933
BsiHKCI	CYCGRG	2 cut(s)	343, 1432
BsiSI	CCGG	7 cut(s)	344, 419, 563, 701, 1433, 1719, 1793
BslFI	GGGAC	6 cut(s)	1190, 1265, 1368, 1449, 1643, 1750
BslI	CCNNNNNNNGG	6 cut(s)	106, 825, 942, 1266, 1352, 1792
BsmAI	GTCTC	2 cut(s)	115, 593
BsmFI	GGGAC	6 cut(s)	1190, 1265, 1368, 1449, 1643, 1750
BsnI	GGCC	4 cut(s)	35, 580, 1008, 1569
Bso31I	GGTCTC	1 cut(s)	115
BsoBI	CYCGRG	2 cut(s)	343, 1432
Bsp1286I	GDGCHC	6 cut(s)	369, 496, 616, 772, 857, 1933
Bsp1407I	TGTACA	1 cut(s)	954
Bsp143I	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
Bsp1720I	GCTNAGC	1 cut(s)	771
Bsp19I	CCATGG	1 cut(s)	1346
BspACI	CCGC	7 cut(s)	18, 36, 217, 395, 428, 566, 2240
BspANI	GGCC	4 cut(s)	35, 580, 1008, 1569
BspCNI	CTCAG	2 cut(s)	784, 797
BspFNI	CGCG	1 cut(s)	395
BspHI	TCATGA	1 cut(s)	1323
BspLI	GGNNCC	3 cut(s)	225, 561, 854
BspMI	ACCTGC	2 cut(s)	85, 244
BspPI	GGATC	4 cut(s)	406, 554, 567, 1994
BspTNI	GGTCTC	1 cut(s)	115
BsrDI	GCAATG	1 cut(s)	879
BsrFI	RCCGGY	1 cut(s)	418
BsrGI	TGTACA	1 cut(s)	954
BsrI	ACTGG	5 cut(s)	675, 944, 1042, 1916, 2006
BssAI	RCCGGY	1 cut(s)	418
BssECI	CCNNGG	8 cut(s)	299, 343, 793, 1346, 1431, 1432, 1805, 2280
BssMI	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
BssSI	CACGAG	2 cut(s)	175, 230
BssT1I	CCWWGG	3 cut(s)	1346, 1805, 2280
Bst2BI	CACGAG	2 cut(s)	175, 230
Bst2UI	CCWGG	2 cut(s)	1479, 2227
Bst4CI	ACNGT	9 cut(s)	109, 128, 464, 794, 895, 1726, 1762, 2050, 2255
Bst6I	CTCTTC	2 cut(s)	716, 988
BstAPI	GCANNNNNTGC	2 cut(s)	1376, 2027
BstAUI	TGTACA	1 cut(s)	954
BstBAI	YACGTR	2 cut(s)	370, 553
BstC8I	GCNNGC	1 cut(s)	1062
BstDEI	CTNAG	4 cut(s)	22, 756, 771, 784
BstDSI	CCRYGG	2 cut(s)	793, 1346
BstENI	CCTNNNNNAGG	1 cut(s)	940
BstF5I	GGATG	2 cut(s)	1336, 2276
BstFNI	CGCG	1 cut(s)	395
BstKTI	GATC	7 cut(s)	54, 273, 401, 562, 864, 1989, 2299
BstMAI	GTCTC	2 cut(s)	115, 593
BstMBI	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
BstMCI	CGRYCG	2 cut(s)	90, 273
BstMWI	GCNNNNNNNGC	6 cut(s)	491, 1376, 1640, 2027, 2159, 2168
BstNI	CCWGG	2 cut(s)	1479, 2227
BstNSI	RCATGY	2 cut(s)	1060, 1157
BstPAI	GACNNNNGTC	1 cut(s)	1800
BstSCI	CCNGG	8 cut(s)	342, 343, 700, 1431, 1432, 1477, 1791, 2225
BstSFI	CTRYAG	3 cut(s)	162, 1710, 1758
BstSLI	GKGCMC	2 cut(s)	496, 1933
BstUI	CGCG	1 cut(s)	395
BstV1I	GCAGC	9 cut(s)	25, 282, 690, 1117, 1379, 1630, 1737, 2014, 2137
BstV2I	GAAGAC	2 cut(s)	126, 1822
BstX2I	RGATCY	2 cut(s)	398, 559
BstYI	RGATCY	2 cut(s)	398, 559
BsuI	GTATCC	3 cut(s)	893, 1073, 1511
BsuRI	GGCC	4 cut(s)	35, 580, 1008, 1569
BtgI	CCRYGG	2 cut(s)	793, 1346
BtgZI	GCGATG	2 cut(s)	584, 889
BtrI	CACGTC	1 cut(s)	79
BtsCI	GGATG	2 cut(s)	1336, 2276
BtsI	GCAGTG	2 cut(s)	702, 1836
BtsIMutI	CAGTG	6 cut(s)	668, 702, 1767, 1836, 2013, 2251
BveI	ACCTGC	2 cut(s)	85, 244
Cac8I	GCNNGC	1 cut(s)	1062
CciI	TCATGA	1 cut(s)	1323
Cfr10I	RCCGGY	1 cut(s)	418
Cfr13I	GGNCC	4 cut(s)	97, 416, 1865, 1937
Cfr9I	CCCGGG	2 cut(s)	343, 1432
CpoI	CGGWCCG	1 cut(s)	416
CseI	GACGC	2 cut(s)	301, 1640
Csp6I	GTAC	7 cut(s)	214, 550, 691, 955, 1456, 2131, 2235
CspI	CGGWCCG	1 cut(s)	416
CviQI	GTAC	7 cut(s)	214, 550, 691, 955, 1456, 2131, 2235
DdeI	CTNAG	4 cut(s)	22, 756, 771, 784
DpnI	GATC	7 cut(s)	53, 272, 400, 561, 863, 1988, 2298
DpnII	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
EaeI	YGGCCR	1 cut(s)	1006
Eam1104I	CTCTTC	2 cut(s)	716, 988
EarI	CTCTTC	2 cut(s)	716, 988
Ecl136II	GAGCTC	1 cut(s)	770
Eco130I	CCWWGG	3 cut(s)	1346, 1805, 2280
Eco24I	GRGCYC	3 cut(s)	616, 772, 857
Eco31I	GGTCTC	1 cut(s)	115
Eco32I	GATATC	3 cut(s)	237, 474, 1484
Eco47I	GGWCC	4 cut(s)	97, 416, 1865, 1937
Eco53kI	GAGCTC	1 cut(s)	770
Eco72I	CACGTG	1 cut(s)	370
Eco88I	CYCGRG	2 cut(s)	343, 1432
EcoICRI	GAGCTC	1 cut(s)	770
EcoNI	CCTNNNNNAGG	1 cut(s)	940
EcoRII	CCWGG	2 cut(s)	1477, 2225
EcoRV	GATATC	3 cut(s)	237, 474, 1484
EcoT14I	CCWWGG	3 cut(s)	1346, 1805, 2280
EcoT38I	GRGCYC	3 cut(s)	616, 772, 857
ErhI	CCWWGG	3 cut(s)	1346, 1805, 2280
FalI	AAGNNNNNCTT	2 cut(s)	461, 493
FaqI	GGGAC	6 cut(s)	1190, 1265, 1368, 1449, 1643, 1750
FauI	CCCGC	1 cut(s)	388
FauNDI	CATATG	3 cut(s)	170, 1114, 1772
FbaI	TGATCA	1 cut(s)	861
FblI	GTMKAC	1 cut(s)	2264
FokI	GGATG	2 cut(s)	1343, 2263
FriOI	GRGCYC	3 cut(s)	616, 772, 857
FspBI	CTAG	7 cut(s)	288, 582, 821, 1307, 1800, 1974, 2156
GsaI	CCCAGC	2 cut(s)	14, 1263
GsuI	CTGGAG	2 cut(s)	961, 2184
HaeIII	GGCC	4 cut(s)	35, 580, 1008, 1569
HapII	CCGG	7 cut(s)	344, 419, 563, 701, 1433, 1719, 1793
HgaI	GACGC	2 cut(s)	301, 1640
HindIII	AAGCTT	1 cut(s)	1965
HinfI	GANTC	7 cut(s)	998, 1356, 1582, 1875, 1893, 1905, 2079
HpaII	CCGG	7 cut(s)	344, 419, 563, 701, 1433, 1719, 1793
HphI	GGTGA	1 cut(s)	883
Hpy166II	GTNNAC	5 cut(s)	159, 797, 1931, 2053, 2265
Hpy188III	TCNNGA	5 cut(s)	142, 232, 274, 645, 1324
Hpy8I	GTNNAC	5 cut(s)	159, 797, 1931, 2053, 2265
Hpy99I	CGWCG	2 cut(s)	74, 899
HpyAV	CCTTC	6 cut(s)	214, 487, 880, 1167, 1321, 2012
HpyCH4III	ACNGT	9 cut(s)	109, 128, 464, 794, 895, 1726, 1762, 2050, 2255
HpyCH4IV	ACGT	6 cut(s)	78, 146, 369, 552, 1458, 2214
HpyF10VI	GCNNNNNNNGC	6 cut(s)	491, 1376, 1640, 2027, 2159, 2168
HpyF3I	CTNAG	4 cut(s)	22, 756, 771, 784
HpySE526I	ACGT	6 cut(s)	78, 146, 369, 552, 1458, 2214
Ksp22I	TGATCA	1 cut(s)	861
Kzo9I	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
LmnI	GCTCC	3 cut(s)	223, 852, 2018
Lsp1109I	GCAGC	9 cut(s)	25, 282, 690, 1117, 1379, 1630, 1737, 2014, 2137
LweI	GCATC	3 cut(s)	1326, 1850, 2149
MaeI	CTAG	7 cut(s)	288, 582, 821, 1307, 1800, 1974, 2156
MaeII	ACGT	6 cut(s)	78, 146, 369, 552, 1458, 2214
MaeIII	GTNAC	2 cut(s)	1426, 1530
MalI	GATC	7 cut(s)	53, 272, 400, 561, 863, 1988, 2298
MboI	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
MflI	RGATCY	2 cut(s)	398, 559
MhlI	GDGCHC	6 cut(s)	369, 496, 616, 772, 857, 1933
MlsI	TGGCCA	1 cut(s)	1008
MluNI	TGGCCA	1 cut(s)	1008
MlyI	GAGTC	3 cut(s)	1350, 1887, 1899
MmeI	TCCRAC	6 cut(s)	138, 725, 877, 1843, 2056, 2297
Mox20I	TGGCCA	1 cut(s)	1008
MroXI	GAANNNNTTC	1 cut(s)	329
MscI	TGGCCA	1 cut(s)	1008
MseI	TTAA	5 cut(s)	354, 1235, 1560, 2201, 2317
MslI	CAYNNNNRTG	7 cut(s)	673, 1154, 1322, 1526, 1595, 2180, 2304
Msp20I	TGGCCA	1 cut(s)	1008
MspA1I	CMGCKG	4 cut(s)	38, 1263, 2064, 2240
MspI	CCGG	7 cut(s)	344, 419, 563, 701, 1433, 1719, 1793
MspR9I	CCNGG	8 cut(s)	344, 345, 702, 1433, 1434, 1479, 1793, 2227
MvaI	CCWGG	2 cut(s)	1479, 2227
MvnI	CGCG	1 cut(s)	395
MwoI	GCNNNNNNNGC	6 cut(s)	491, 1376, 1640, 2027, 2159, 2168
NciI	CCSGG	6 cut(s)	344, 345, 702, 1433, 1434, 1793
NcoI	CCATGG	1 cut(s)	1346
NdeI	CATATG	3 cut(s)	170, 1114, 1772
NdeII	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
NlaIV	GGNNCC	3 cut(s)	225, 561, 854
NmeAIII	GCCGAG	1 cut(s)	324
NmuCI	GTSAC	1 cut(s)	1530
NspI	RCATGY	2 cut(s)	1060, 1157
OliI	CACNNNNGTG	1 cut(s)	1154
PagI	TCATGA	1 cut(s)	1323
PaqCI	CACCTGC	1 cut(s)	244
PciI	ACATGT	1 cut(s)	1153
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PdmI	GAANNNNTTC	1 cut(s)	329
PfeI	GAWTC	4 cut(s)	998, 1582, 1875, 2079
PflMI	CCANNNNNTGG	1 cut(s)	1266
PfoI	TCCNGGA	1 cut(s)	1791
Ple19I	CGATCG	1 cut(s)	273
PleI	GAGTC	3 cut(s)	1350, 1887, 1899
PmaCI	CACGTG	1 cut(s)	370
PmlI	CACGTG	1 cut(s)	370
PpsI	GAGTC	3 cut(s)	1350, 1887, 1899
Ppu21I	YACGTR	2 cut(s)	370, 553
PscI	ACATGT	1 cut(s)	1153
PshAI	GACNNNNGTC	1 cut(s)	1800
PshBI	ATTAAT	1 cut(s)	1235
PsiI	TTATAA	1 cut(s)	1694
Psp124BI	GAGCTC	1 cut(s)	772
Psp6I	CCWGG	2 cut(s)	1477, 2225
PspCI	CACGTG	1 cut(s)	370
PspFI	CCCAGC	2 cut(s)	10, 1259
PspGI	CCWGG	2 cut(s)	1477, 2225
PspN4I	GGNNCC	3 cut(s)	225, 561, 854
PspPI	GGNCC	4 cut(s)	97, 416, 1865, 1937
PsuI	RGATCY	2 cut(s)	398, 559
PvuI	CGATCG	1 cut(s)	273
PvuII	CAGCTG	2 cut(s)	1263, 2064
RsaI	GTAC	7 cut(s)	215, 551, 692, 956, 1457, 2132, 2236
RsaNI	GTAC	7 cut(s)	214, 550, 691, 955, 1456, 2131, 2235
RseI	CAYNNNNRTG	7 cut(s)	673, 1154, 1322, 1526, 1595, 2180, 2304
Rsr2I	CGGWCCG	1 cut(s)	416
RsrII	CGGWCCG	1 cut(s)	416
SacI	GAGCTC	1 cut(s)	772
SaqAI	TTAA	5 cut(s)	354, 1235, 1560, 2201, 2317
Sau3AI	GATC	7 cut(s)	51, 270, 398, 559, 861, 1986, 2296
Sau96I	GGNCC	4 cut(s)	97, 416, 1865, 1937
SchI	GAGTC	3 cut(s)	1350, 1887, 1899
ScrFI	CCNGG	8 cut(s)	344, 345, 702, 1433, 1434, 1479, 1793, 2227
SduI	GDGCHC	6 cut(s)	369, 496, 616, 772, 857, 1933
SfaNI	GCATC	3 cut(s)	1326, 1850, 2149
SfcI	CTRYAG	3 cut(s)	162, 1710, 1758
SinI	GGWCC	4 cut(s)	97, 416, 1865, 1937
SmaI	CCCGGG	2 cut(s)	345, 1434
SmiMI	CAYNNNNRTG	7 cut(s)	673, 1154, 1322, 1526, 1595, 2180, 2304
SmlI	CTYRAG	1 cut(s)	1090
SmoI	CTYRAG	1 cut(s)	1090
SpeI	ACTAGT	1 cut(s)	1799
SsiI	CCGC	7 cut(s)	18, 36, 217, 395, 428, 566, 2240
SspI	AATATT	1 cut(s)	1732
SspMI	CTAG	7 cut(s)	288, 582, 821, 1307, 1800, 1974, 2156
SstI	GAGCTC	1 cut(s)	772
StyD4I	CCNGG	8 cut(s)	342, 343, 700, 1431, 1432, 1477, 1791, 2225
StyI	CCWWGG	3 cut(s)	1346, 1805, 2280
TaaI	ACNGT	9 cut(s)	109, 128, 464, 794, 895, 1726, 1762, 2050, 2255
TaiI	ACGT	6 cut(s)	81, 149, 372, 555, 1461, 2217
TaqI	TCGA	6 cut(s)	54, 90, 380, 637, 996, 2295
TaqII	GACCGA	3 cut(s)	76, 820, 1882
TatI	WGTACW	1 cut(s)	954
TauI	GCSGC	2 cut(s)	38, 569
TfiI	GAWTC	4 cut(s)	998, 1582, 1875, 2079
Tru1I	TTAA	5 cut(s)	354, 1235, 1560, 2201, 2317
Tru9I	TTAA	5 cut(s)	354, 1235, 1560, 2201, 2317
TscAI	CASTG	6 cut(s)	675, 702, 1767, 1843, 2013, 2258
TseFI	GTSAC	1 cut(s)	1530
TseI	GCWGC	9 cut(s)	38, 295, 678, 1130, 1367, 1643, 1750, 2027, 2150
Tsp45I	GTSAC	1 cut(s)	1530
TspGWI	ACGGA	7 cut(s)	89, 140, 236, 238, 429, 1466, 1914
TspMI	CCCGGG	2 cut(s)	343, 1432
TspRI	CASTG	6 cut(s)	675, 702, 1767, 1843, 2013, 2258
Van91I	CCANNNNNTGG	1 cut(s)	1266
VneI	GTGCAC	1 cut(s)	1929
VpaK11BI	GGWCC	4 cut(s)	97, 416, 1865, 1937
VspI	ATTAAT	1 cut(s)	1235
XagI	CCTNNNNNAGG	1 cut(s)	940
XapI	RAATTY	3 cut(s)	350, 653, 967
XceI	RCATGY	2 cut(s)	1060, 1157
XmaI	CCCGGG	2 cut(s)	343, 1432
XmiI	GTMKAC	1 cut(s)	2264
XmnI	GAANNNNTTC	1 cut(s)	329
XspI	CTAG	7 cut(s)	288, 582, 821, 1307, 1800, 1974, 2156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.