Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0003716

Physical Location & Seq

Genomic Coordinates Forward (+)

60143317 .. 60147103

Gene Structure 3787 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG425900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGACATAGAGTCCACCAAACTCGACGTGGACGACGACGTGTCACCCATCGAAGAGGTCCGCTTAACGGTCTCGAACGAAGACGACCCGACCCTTCCAGTATGGACCTTCCGGATGTGGACCCTAGGGATCCTCTCCTGCGTCCTCCTCTCCTTCCTCAACACCTTCTTCTCCTACCGCACCGAGCCCCTGATCATCACCATGATCACCGTACAGGTGGCGACACTGCCCATCGGACGGTTCATGGCTAGGGTACTGCCCACTAGGAAGTTTTCGATCGGGTCGATGGAGTTCTCGCTCAACCCGGGTCCGTTTAACAAGAAGGAGCACGTGCTGATATCCATATTTGCCAATGCCGGATCTGGGTTCGGAAGCGGAACGGCTTATGCTGTTGGCATTGTCGATATAATTAAGGCATTTTATCACAGGAAGATATCGTTCTTGGCCAGTTGGCTTCTTGTCAATACCACACAGGCATTGGGGTATGGGTGGGCTGGAATTCTCCGAAAGTACGTGGTGGATCCGGGAGAGATGTGGTGGCCCTCTAGTGTAGTTCAGGTCAACTTGTTCGATGCCCTACACGAGAAAGACAACAAGCGAATGTCCAGAGCGAAATTCTTCTTAATTGCATTCATATGCAGCTTCTCTTGGTATGTTGTCCCAGGCTACCTTTTCCCAACTATATCAACCATTTCGTGGATTTGTTGGATATACCCCAAGTCAGTAACAGTGCAACAAATTGCATCCGGCATGCGTGGACTTGGTGTTGGTGCATTGTCTCTCGACTGGTCTGTTGTAGCCTCGTACCTTCAAAGCCCTTTGATTAGCCCTTTCTTTGCCACTGTCAATGTTGCAGTTGGCTATGTTGCTTTCATGTTTGTCATACTGCCAATTGCCTACTGGGGAGTCAATTTATACAATGCCAAGAACTTCCCAATCTTCTCTTCCCATTTGTTTGATGCCCAAGGTCAAGTATATAACACTTCAGCAATTGTGAATGACAAATTTCAAATTGACATGCCGGCGTATGAAAAACAAGGACGTGTAAACTTAAGTGTGTTTTTTGCTCTGACATATGGTATTGGCTTTGCGGCCATTATATCAACCCTCACTCACGTGGCCCTCTTCAATGGAAGGGAGATATATGAACAATTTCGAGCATCGAAGAAGGATGGAAAGGTAGACATACACACCAAATTGATGAGGAAATACAAGGACATACCTAACTGGTGGTTTCACCTTACTCTTTTGGTGTCATTTGGAATCGCTCTGGCACTATGCATTTTCATGGAAGATGAGATTCCAATGCCGTGGTGGGCACTCATTTTCGCTGCAGTCCTTGCATTAATTTTCACTCTTCCGGTCAGCATTATTACCGCCACAACAAATCAGACGCCGGGACTAAACATTATCACAGAGTACATCATGGGATTGATTTTACCCGGAAAACCGATTCTTAATGTATGCTTCAAAACCTATGGTTATATTAGCATGAGTCAGGCAATTTCCTTTCTCAATGATTTCAAGCTTGGCCATTACATGAAGATTCCGCCCAGATCAATGTTTTTAGTTCAGTTGATAGGAACTATTATAGCTGGAACAGTCAATATCGGGGTTGCGTGGTGGCTGTTATCTACTGTGGAAAACATTTGTCAAGATCAATTACTCCCTCCTAATAGTCCTTGGACTTGTCCTGGTGATCGCGTCTTCTTTGATGCATCTGTCGTTTGGGGTCTTGTCGGTCCAAAGCGGATATTTGGTCCTCTTGGAAACTACCAAGCACTCAACTGGTTCTTCCTTATAGGTGCATTTGGACCACTCTTGGTATGGTTGTTACACAGAGCTTTTCCCAGTTATAAATGGATTGGACTTATCAACCTTCCAGTGCTTTTGAATGCAACAGCTGCAATGCCACCGGCTACATCAGTAAACTTCAATTGCTGGATTGTTATTGGAACTATTTTCGGCTACTACATCTTTAGATATAGAACGGCATGGTGGCAGAGGTACAATTATGTTCTTTCGGCTGCCTTAGACGCTGGATTGGCTTGCATGGCGGTGCTCTTATACTTCACATTAACCATGAACGGGAAGAGCATATCTTGGTGGGGTGCGGATGGTGAACACTGTGAATTGGCTTCATGTCCCACAGCCAAAGGAATCGTTGTTGATGGTTGTCCTGTCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006857 GO:0008150 GO:0015833 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0035672 GO:0035673 GO:0042886 GO:0042887 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:1904680

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OPT

Protein Analysis

729

Amino Acids

81.73

Weight (kDa)

8.55

Isoelectric Point (pI)

29.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OPT	PF03169	34 - 692	1.5e-184	OPT oligopeptide transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09930
fragaria_vesca	FvH4_5g34010 FvH4_5g34011
malus_domestica	MD08G1206700.v1.1 MD15G1390200.v1.1 MD15G1390400.v1.1
prunus_persica	Prupe.1G539200_v2.0.a1 Prupe.1G539300_v2.0.a1
pyrus_communis	pycom15g34950 pycom15g34960
rosa_chinensis	RchiOBHm_Chr7g0233451 RchiOBHm_Chr7g0233471
rosa_laevigata	RLG00000001268 RLG00000001269
rosa_multiflora	Rmu_sc0000029.1_g000038 Rmu_sc0000029.1_g000051
rosa_roxburghii	Rroxscaffold_3G00227270 Rroxscaffold_3G00227290
rosa_rugosa	Rorug07G0280800 Rorug07G0280800
rosa_samantha	Rh7AG436600 Rh7AG436700 Rh7BG409700 Rh7BG409800 Rh7CG456500 Rh7CG456700 Rh7DG425800 Rh7DG425900
rosa_wichuraiana	Rw0G008530 Rw0G022500 Rw7G036000 Rw7G036010 Rw7G036030 Rw7G036040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1857
AccB7I	CCANNNNNTGG	1 cut(s)	696
AccI	GTMKAC	1 cut(s)	1182
AccII	CGCG	1 cut(s)	1704
AccIII	TCCGGA	1 cut(s)	111
AciI	CCGC	9 cut(s)	61, 178, 375, 1091, 1377, 1550, 1750, 2057, 2114
AclWI	GGATC	5 cut(s)	124, 137, 367, 515, 528
AcoI	YGGCCR	3 cut(s)	444, 1092, 1531
AcsI	RAATTY	3 cut(s)	498, 614, 1004
AcuI	CTGAAG	1 cut(s)	969
AcvI	CACGTG	2 cut(s)	331, 1117
AcyI	GRCGYC	1 cut(s)	1394
AfaI	GTAC	6 cut(s)	213, 255, 512, 806, 1421, 2009
AfiI	CCNNNNNNNGG	5 cut(s)	67, 237, 363, 696, 2088
AflII	CTTAAG	1 cut(s)	1051
AflIII	ACRYGT	2 cut(s)	39, 1042
AgsI	TTSAA	7 cut(s)	812, 1010, 1129, 1471, 1525, 1894, 1936
AhdI	GACNNNNNGTC	2 cut(s)	10, 40
AjiI	CACGTC	3 cut(s)	28, 40, 1043
AjnI	CCWGG	2 cut(s)	661, 1693
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
AleI	CACNNNNGTG	1 cut(s)	1115
AluBI	AGCT	5 cut(s)	642, 1528, 1595, 1844, 1904
AluI	AGCT	5 cut(s)	642, 1528, 1595, 1844, 1904
Alw21I	GWGCWC	2 cut(s)	330, 2064
Alw26I	GTCTC	2 cut(s)	76, 783
AlwI	GGATC	5 cut(s)	124, 137, 367, 515, 528
Ama87I	CYCGRG	1 cut(s)	304
Aor13HI	TCCGGA	1 cut(s)	111
AoxI	GGCC	5 cut(s)	444, 539, 1092, 1119, 1531
ApeKI	GCWGC	4 cut(s)	639, 1331, 1904, 2027
ApoI	RAATTY	3 cut(s)	498, 614, 1004
AseI	ATTAAT	1 cut(s)	1346
Asp700I	GAANNNNTTC	2 cut(s)	617, 1152
AspA2I	CCTAGG	1 cut(s)	124
AspS9I	GGNCC	9 cut(s)	58, 105, 120, 308, 540, 1120, 1742, 1760, 1814
AsuC2I	CCSGG	5 cut(s)	305, 306, 525, 1398, 1443
AsuHPI	GGTGA	6 cut(s)	36, 190, 199, 1229, 1709, 2132
AvaI	CYCGRG	1 cut(s)	304
AvaII	GGWCC	7 cut(s)	58, 105, 120, 308, 1742, 1760, 1814
AvrII	CCTAGG	1 cut(s)	124
BaeGI	GKGCMC	1 cut(s)	1321
BalI	TGGCCA	2 cut(s)	446, 1533
BamHI	GGATCC	2 cut(s)	129, 520
BanII	GRGCYC	1 cut(s)	189
BauI	CACGAG	1 cut(s)	581
BbrPI	CACGTG	2 cut(s)	331, 1117
BbsI	GAAGAC	3 cut(s)	87, 1699, 2176
Bbv12I	GWGCWC	2 cut(s)	330, 2064
BbvI	GCAGC	4 cut(s)	651, 1318, 1891, 2014
BccI	CCATC	6 cut(s)	56, 239, 280, 1166, 2111, 2165
BceAI	ACGGC	3 cut(s)	396, 1294, 2007
BciT130I	CCWGG	2 cut(s)	663, 1695
BclI	TGATCA	2 cut(s)	192, 204
BcnI	CCSGG	5 cut(s)	305, 306, 525, 1398, 1443
BcoDI	GTCTC	2 cut(s)	76, 783
BfaI	CTAG	4 cut(s)	125, 249, 264, 546
BfmI	CTRYAG	1 cut(s)	1332
BfrI	CTTAAG	1 cut(s)	1051
BisI	GCNGC	5 cut(s)	640, 1092, 1332, 1905, 2028
BlnI	CCTAGG	1 cut(s)	124
BlsI	GCNGC	5 cut(s)	641, 1093, 1333, 1906, 2029
Bme1390I	CCNGG	7 cut(s)	305, 306, 525, 663, 1398, 1443, 1695
Bme18I	GGWCC	7 cut(s)	58, 105, 120, 308, 1742, 1760, 1814
BmeRI	GACNNNNNGTC	2 cut(s)	10, 40
BmeT110I	CYCGRG	1 cut(s)	304
BmgBI	CACGTC	3 cut(s)	28, 40, 1043
BmgT120I	GGNCC	9 cut(s)	58, 105, 120, 308, 540, 1120, 1742, 1760, 1814
BmiI	GGNNCC	4 cut(s)	122, 131, 309, 522
BmrFI	CCNGG	7 cut(s)	305, 306, 525, 663, 1398, 1443, 1695
BmrI	ACTGGG	2 cut(s)	910, 1845
BmsI	GCATC	6 cut(s)	562, 752, 949, 1169, 1705, 1727
BmuI	ACTGGG	2 cut(s)	910, 1845
BpiI	GAAGAC	3 cut(s)	87, 1699, 2176
BpuMI	CCSGG	5 cut(s)	305, 306, 525, 1398, 1443
BsaAI	YACGTR	3 cut(s)	331, 514, 1117
BsaHI	GRCGYC	1 cut(s)	1394
BsaI	GGTCTC	1 cut(s)	76
BsaJI	CCNNGG	6 cut(s)	124, 304, 661, 964, 1310, 1682
BsaWI	WCCGGW	2 cut(s)	111, 1360
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 237, 363, 696, 2088
Bse118I	RCCGGY	2 cut(s)	1021, 1915
Bse1I	ACTGG	8 cut(s)	98, 447, 791, 905, 1232, 1793, 1851, 1883
Bse3DI	GCAATG	1 cut(s)	1914
BseAI	TCCGGA	1 cut(s)	111
BseBI	CCWGG	2 cut(s)	663, 1695
BseDI	CCNNGG	6 cut(s)	124, 304, 661, 964, 1310, 1682
BseGI	GGATG	4 cut(s)	120, 743, 1177, 2122
BseLI	CCNNNNNNNGG	5 cut(s)	67, 237, 363, 696, 2088
BseMI	GCAATG	1 cut(s)	1914
BseNI	ACTGG	8 cut(s)	98, 447, 791, 905, 1232, 1793, 1851, 1883
BseRI	GAGGAG	1 cut(s)	137
BseSI	GKGCMC	1 cut(s)	1321
BseXI	GCAGC	4 cut(s)	651, 1318, 1891, 2014
Bsh1236I	CGCG	1 cut(s)	1704
Bsh1285I	CGRYCG	1 cut(s)	279
BshFI	GGCC	5 cut(s)	446, 541, 1094, 1121, 1533
BsiEI	CGRYCG	1 cut(s)	279
BsiHKAI	GWGCWC	2 cut(s)	330, 2064
BsiHKCI	CYCGRG	1 cut(s)	304
BslFI	GGGAC	3 cut(s)	644, 1413, 2130
BslI	CCNNNNNNNGG	5 cut(s)	67, 237, 363, 696, 2088
BsmAI	GTCTC	2 cut(s)	76, 783
BsmFI	GGGAC	3 cut(s)	644, 1413, 2130
BsmI	GAATGC	2 cut(s)	629, 1900
BsnI	GGCC	5 cut(s)	446, 541, 1094, 1121, 1533
Bso31I	GGTCTC	1 cut(s)	76
BsoBI	CYCGRG	1 cut(s)	304
Bsp1286I	GDGCHC	4 cut(s)	189, 330, 1321, 2064
Bsp13I	TCCGGA	1 cut(s)	111
Bsp143I	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
BspACI	CCGC	9 cut(s)	61, 178, 375, 1091, 1377, 1550, 1750, 2057, 2114
BspANI	GGCC	5 cut(s)	446, 541, 1094, 1121, 1533
BspEI	TCCGGA	1 cut(s)	111
BspFNI	CGCG	1 cut(s)	1704
BspLI	GGNNCC	4 cut(s)	122, 131, 309, 522
BspMAI	CTGCAG	1 cut(s)	1336
BspPI	GGATC	5 cut(s)	124, 137, 367, 515, 528
BspQI	GCTCTTC	1 cut(s)	2087
BspTI	CTTAAG	1 cut(s)	1051
BspTNI	GGTCTC	1 cut(s)	76
BsrDI	GCAATG	1 cut(s)	1914
BsrFI	RCCGGY	2 cut(s)	1021, 1915
BsrI	ACTGG	8 cut(s)	98, 447, 791, 905, 1232, 1793, 1851, 1883
BssAI	RCCGGY	2 cut(s)	1021, 1915
BssECI	CCNNGG	6 cut(s)	124, 304, 661, 964, 1310, 1682
BssMI	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
BssNI	GRCGYC	1 cut(s)	1394
BssSI	CACGAG	1 cut(s)	581
BssT1I	CCWWGG	3 cut(s)	124, 964, 1682
Bst2BI	CACGAG	1 cut(s)	581
Bst2UI	CCWGG	2 cut(s)	663, 1695
Bst4CI	ACNGT	8 cut(s)	70, 211, 240, 730, 844, 1603, 1639, 2129
Bst6I	CTCTTC	5 cut(s)	48, 949, 1130, 1362, 2087
BstACI	GRCGYC	1 cut(s)	1394
BstAFI	CTTAAG	1 cut(s)	1051
BstAPI	GCANNNNNTGC	2 cut(s)	1340, 1904
BstBAI	YACGTR	3 cut(s)	331, 514, 1117
BstC8I	GCNNGC	3 cut(s)	752, 1023, 2050
BstDEI	CTNAG	1 cut(s)	2032
BstDSI	CCRYGG	1 cut(s)	1310
BstF5I	GGATG	4 cut(s)	120, 743, 1177, 2122
BstFNI	CGCG	1 cut(s)	1704
BstKTI	GATC	9 cut(s)	132, 195, 207, 279, 362, 523, 1559, 1660, 1702
BstMAI	GTCTC	2 cut(s)	76, 783
BstMBI	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
BstMCI	CGRYCG	1 cut(s)	279
BstMWI	GCNNNNNNNGC	5 cut(s)	1340, 1904, 2036, 2045, 2054
BstNI	CCWGG	2 cut(s)	663, 1695
BstNSI	RCATGY	2 cut(s)	754, 1021
BstSCI	CCNGG	7 cut(s)	303, 304, 523, 661, 1396, 1441, 1693
BstSFI	CTRYAG	1 cut(s)	1332
BstSLI	GKGCMC	1 cut(s)	1321
BstUI	CGCG	1 cut(s)	1704
BstV1I	GCAGC	4 cut(s)	651, 1318, 1891, 2014
BstV2I	GAAGAC	3 cut(s)	87, 1699, 2176
BstX2I	RGATCY	3 cut(s)	129, 359, 520
BstXI	CCANNNNNNTGG	1 cut(s)	1311
BstYI	RGATCY	3 cut(s)	129, 359, 520
BsuRI	GGCC	5 cut(s)	446, 541, 1094, 1121, 1533
BtgI	CCRYGG	1 cut(s)	1310
BtrI	CACGTC	3 cut(s)	28, 40, 1043
BtsCI	GGATG	4 cut(s)	120, 743, 1177, 2122
BtsI	GCAGTG	1 cut(s)	224
BtsIMutI	CAGTG	5 cut(s)	224, 735, 840, 1890, 2125
Cac8I	GCNNGC	3 cut(s)	752, 1023, 2050
Cfr10I	RCCGGY	2 cut(s)	1021, 1915
Cfr13I	GGNCC	9 cut(s)	58, 105, 120, 308, 540, 1120, 1742, 1760, 1814
Cfr9I	CCCGGG	1 cut(s)	304
CseI	GACGC	4 cut(s)	130, 1402, 1693, 2045
Csp6I	GTAC	6 cut(s)	212, 254, 511, 805, 1420, 2008
CviQI	GTAC	6 cut(s)	212, 254, 511, 805, 1420, 2008
DdeI	CTNAG	1 cut(s)	2032
DpnI	GATC	9 cut(s)	131, 194, 206, 278, 361, 522, 1558, 1659, 1701
DpnII	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
DriI	GACNNNNNGTC	2 cut(s)	10, 40
EaeI	YGGCCR	3 cut(s)	444, 1092, 1531
Eam1104I	CTCTTC	5 cut(s)	48, 949, 1130, 1362, 2087
Eam1105I	GACNNNNNGTC	2 cut(s)	10, 40
EarI	CTCTTC	5 cut(s)	48, 949, 1130, 1362, 2087
EciI	GGCGGA	1 cut(s)	1539
Eco130I	CCWWGG	3 cut(s)	124, 964, 1682
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	1 cut(s)	76
Eco32I	GATATC	2 cut(s)	339, 435
Eco47I	GGWCC	7 cut(s)	58, 105, 120, 308, 1742, 1760, 1814
Eco57I	CTGAAG	1 cut(s)	969
Eco72I	CACGTG	2 cut(s)	331, 1117
Eco88I	CYCGRG	1 cut(s)	304
EcoRI	GAATTC	1 cut(s)	498
EcoRII	CCWGG	2 cut(s)	661, 1693
EcoRV	GATATC	2 cut(s)	339, 435
EcoT14I	CCWWGG	3 cut(s)	124, 964, 1682
EcoT22I	ATGCAT	2 cut(s)	1283, 1720
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	3 cut(s)	124, 964, 1682
FaqI	GGGAC	3 cut(s)	644, 1413, 2130
FauNDI	CATATG	2 cut(s)	635, 1075
FbaI	TGATCA	2 cut(s)	192, 204
FblI	GTMKAC	1 cut(s)	1182
Fnu4HI	GCNGC	5 cut(s)	640, 1092, 1332, 1905, 2028
FokI	GGATG	4 cut(s)	127, 730, 1184, 2129
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	5 cut(s)	640, 1092, 1332, 1905, 2028
FspBI	CTAG	4 cut(s)	125, 249, 264, 546
GluI	GCNGC	5 cut(s)	640, 1092, 1332, 1905, 2028
HaeIII	GGCC	5 cut(s)	446, 541, 1094, 1121, 1533
HgaI	GACGC	4 cut(s)	130, 1402, 1693, 2045
Hin1I	GRCGYC	1 cut(s)	1394
HincII	GTYRAC	1 cut(s)	562
HindII	GTYRAC	1 cut(s)	562
HindIII	AAGCTT	1 cut(s)	1526
HinfI	GANTC	8 cut(s)	11, 906, 1263, 1300, 1453, 1495, 1546, 2160
HphI	GGTGA	6 cut(s)	36, 190, 199, 1229, 1709, 2132
Hpy166II	GTNNAC	9 cut(s)	15, 31, 120, 562, 758, 1048, 1183, 1930, 2123
Hpy188I	TCNGA	5 cut(s)	236, 371, 506, 1071, 1392
Hpy188III	TCNNGA	5 cut(s)	73, 112, 606, 782, 1655
Hpy8I	GTNNAC	9 cut(s)	15, 31, 120, 562, 758, 1048, 1183, 1930, 2123
Hpy99I	CGWCG	3 cut(s)	29, 38, 41
HpyAV	CCTTC	9 cut(s)	104, 118, 163, 175, 316, 818, 1128, 1162, 1889
HpyCH4III	ACNGT	8 cut(s)	70, 211, 240, 730, 844, 1603, 1639, 2129
HpyCH4IV	ACGT	6 cut(s)	27, 39, 330, 513, 1042, 1116
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1340, 1904, 2036, 2045, 2054
HpyF3I	CTNAG	1 cut(s)	2032
HpySE526I	ACGT	6 cut(s)	27, 39, 330, 513, 1042, 1116
Hsp92I	GRCGYC	1 cut(s)	1394
Kpn2I	TCCGGA	1 cut(s)	111
KroI	GCCGGC	1 cut(s)	1021
KroNI	GCCGGC	1 cut(s)	1023
Ksp22I	TGATCA	2 cut(s)	192, 204
Kzo9I	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
LguI	GCTCTTC	1 cut(s)	2087
LmnI	GCTCC	1 cut(s)	325
Lsp1109I	GCAGC	4 cut(s)	651, 1318, 1891, 2014
LweI	GCATC	6 cut(s)	562, 752, 949, 1169, 1705, 1727
MaeI	CTAG	4 cut(s)	125, 249, 264, 546
MaeII	ACGT	6 cut(s)	27, 39, 330, 513, 1042, 1116
MaeIII	GTNAC	3 cut(s)	42, 724, 1833
MalI	GATC	9 cut(s)	131, 194, 206, 278, 361, 522, 1558, 1659, 1701
MboI	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
MfeI	CAATTG	3 cut(s)	891, 990, 1936
MflI	RGATCY	3 cut(s)	129, 359, 520
MhlI	GDGCHC	4 cut(s)	189, 330, 1321, 2064
MlsI	TGGCCA	2 cut(s)	446, 1533
MluNI	TGGCCA	2 cut(s)	446, 1533
MlyI	GAGTC	3 cut(s)	20, 915, 1504
MmeI	TCCRAC	1 cut(s)	686
Mox20I	TGGCCA	2 cut(s)	446, 1533
Mph1103I	ATGCAT	2 cut(s)	1283, 1720
MroI	TCCGGA	1 cut(s)	111
MroNI	GCCGGC	1 cut(s)	1021
MroXI	GAANNNNTTC	2 cut(s)	617, 1152
MscI	TGGCCA	2 cut(s)	446, 1533
MseI	TTAA	8 cut(s)	65, 315, 411, 623, 1052, 1346, 1458, 2078
MslI	CAYNNNNRTG	5 cut(s)	200, 634, 1115, 1286, 2057
Msp20I	TGGCCA	2 cut(s)	446, 1533
MspA1I	CMGCKG	1 cut(s)	1904
MspCI	CTTAAG	1 cut(s)	1051
MspR9I	CCNGG	7 cut(s)	305, 306, 525, 663, 1398, 1443, 1695
MunI	CAATTG	3 cut(s)	891, 990, 1936
Mva1269I	GAATGC	2 cut(s)	629, 1900
MvaI	CCWGG	2 cut(s)	663, 1695
MvnI	CGCG	1 cut(s)	1704
MwoI	GCNNNNNNNGC	5 cut(s)	1340, 1904, 2036, 2045, 2054
NaeI	GCCGGC	1 cut(s)	1023
NciI	CCSGG	5 cut(s)	305, 306, 525, 1398, 1443
NdeI	CATATG	2 cut(s)	635, 1075
NdeII	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
NgoMIV	GCCGGC	1 cut(s)	1021
NlaIV	GGNNCC	4 cut(s)	122, 131, 309, 522
NmuCI	GTSAC	1 cut(s)	42
NsiI	ATGCAT	2 cut(s)	1283, 1720
NspI	RCATGY	2 cut(s)	754, 1021
OliI	CACNNNNGTG	1 cut(s)	1115
PaeI	GCATGC	1 cut(s)	754
PciSI	GCTCTTC	1 cut(s)	2087
PcsI	WCGNNNNNNNCGW	3 cut(s)	30, 33, 281
PctI	GAATGC	2 cut(s)	629, 1900
PdiI	GCCGGC	1 cut(s)	1023
PdmI	GAANNNNTTC	2 cut(s)	617, 1152
PfeI	GAWTC	5 cut(s)	1263, 1300, 1453, 1546, 2160
PflMI	CCANNNNNTGG	1 cut(s)	696
PfoI	TCCNGGA	1 cut(s)	523
PkrI	GCNGC	5 cut(s)	641, 1093, 1333, 1906, 2029
Ple19I	CGATCG	1 cut(s)	279
PleI	GAGTC	3 cut(s)	19, 914, 1503
PmaCI	CACGTG	2 cut(s)	331, 1117
PmlI	CACGTG	2 cut(s)	331, 1117
PpsI	GAGTC	3 cut(s)	19, 914, 1503
Ppu21I	YACGTR	3 cut(s)	331, 514, 1117
PshBI	ATTAAT	1 cut(s)	1346
PsiI	TTATAA	1 cut(s)	1857
Psp6I	CCWGG	2 cut(s)	661, 1693
PspCI	CACGTG	2 cut(s)	331, 1117
PspGI	CCWGG	2 cut(s)	661, 1693
PspN4I	GGNNCC	4 cut(s)	122, 131, 309, 522
PspPI	GGNCC	9 cut(s)	58, 105, 120, 308, 540, 1120, 1742, 1760, 1814
PstI	CTGCAG	1 cut(s)	1336
PsuI	RGATCY	3 cut(s)	129, 359, 520
PvuI	CGATCG	1 cut(s)	279
PvuII	CAGCTG	1 cut(s)	1904
RsaI	GTAC	6 cut(s)	213, 255, 512, 806, 1421, 2009
RsaNI	GTAC	6 cut(s)	212, 254, 511, 805, 1420, 2008
RseI	CAYNNNNRTG	5 cut(s)	200, 634, 1115, 1286, 2057
SapI	GCTCTTC	1 cut(s)	2087
SaqAI	TTAA	8 cut(s)	65, 315, 411, 623, 1052, 1346, 1458, 2078
SatI	GCNGC	5 cut(s)	640, 1092, 1332, 1905, 2028
Sau3AI	GATC	9 cut(s)	129, 192, 204, 276, 359, 520, 1556, 1657, 1699
Sau96I	GGNCC	9 cut(s)	58, 105, 120, 308, 540, 1120, 1742, 1760, 1814
SchI	GAGTC	3 cut(s)	20, 915, 1504
ScrFI	CCNGG	7 cut(s)	305, 306, 525, 663, 1398, 1443, 1695
SduI	GDGCHC	4 cut(s)	189, 330, 1321, 2064
SfaNI	GCATC	6 cut(s)	562, 752, 949, 1169, 1705, 1727
SfcI	CTRYAG	1 cut(s)	1332
SinI	GGWCC	7 cut(s)	58, 105, 120, 308, 1742, 1760, 1814
SmaI	CCCGGG	1 cut(s)	306
SmiMI	CAYNNNNRTG	5 cut(s)	200, 634, 1115, 1286, 2057
SmlI	CTYRAG	1 cut(s)	1051
SmoI	CTYRAG	1 cut(s)	1051
SphI	GCATGC	1 cut(s)	754
SsiI	CCGC	9 cut(s)	61, 178, 375, 1091, 1377, 1550, 1750, 2057, 2114
SspMI	CTAG	4 cut(s)	125, 249, 264, 546
StyD4I	CCNGG	7 cut(s)	303, 304, 523, 661, 1396, 1441, 1693
StyI	CCWWGG	3 cut(s)	124, 964, 1682
TaaI	ACNGT	8 cut(s)	70, 211, 240, 730, 844, 1603, 1639, 2129
TaiI	ACGT	6 cut(s)	30, 42, 333, 516, 1045, 1119
TaqII	GACCGA	1 cut(s)	1730
TatI	WGTACW	1 cut(s)	1419
TauI	GCSGC	1 cut(s)	1094
TfiI	GAWTC	5 cut(s)	1263, 1300, 1453, 1546, 2160
Tru1I	TTAA	8 cut(s)	65, 315, 411, 623, 1052, 1346, 1458, 2078
Tru9I	TTAA	8 cut(s)	65, 315, 411, 623, 1052, 1346, 1458, 2078
TscAI	CASTG	5 cut(s)	231, 735, 847, 1890, 2132
TseFI	GTSAC	1 cut(s)	42
TseI	GCWGC	4 cut(s)	639, 1331, 1904, 2027
Tsp45I	GTSAC	1 cut(s)	42
TspDTI	ATGAA	9 cut(s)	232, 622, 862, 1044, 1161, 1276, 1556, 2099, 2130
TspGWI	ACGGA	1 cut(s)	300
TspMI	CCCGGG	1 cut(s)	304
TspRI	CASTG	5 cut(s)	231, 735, 847, 1890, 2132
Van91I	CCANNNNNTGG	1 cut(s)	696
Vha464I	CTTAAG	1 cut(s)	1051
VpaK11BI	GGWCC	7 cut(s)	58, 105, 120, 308, 1742, 1760, 1814
VspI	ATTAAT	1 cut(s)	1346
XapI	RAATTY	3 cut(s)	498, 614, 1004
XceI	RCATGY	2 cut(s)	754, 1021
XcmI	CCANNNNNNNNNTGG	5 cut(s)	25, 475, 897, 1311, 1824
XmaI	CCCGGG	1 cut(s)	304
XmaJI	CCTAGG	1 cut(s)	124
XmiI	GTMKAC	1 cut(s)	1182
XmnI	GAANNNNTTC	2 cut(s)	617, 1152
XspI	CTAG	4 cut(s)	125, 249, 264, 546
Zsp2I	ATGCAT	2 cut(s)	1283, 1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.