MD15G1390400.v1.1

oligopeptide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003716
Physical Location & Seq
Reverse (-)
48485937 .. 48493687
7751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1390400.v1.1.491

Sequence Viewer

Length: 2232 bp
ATGGGCACCCAGGTCACCACCCTAGAAGTGGAGGCCGCTGCTAAAACTAAGCTCGATGAGAGTGAGAACGACGATGACGTGTCATCAGTTGAGCAGGTCCGACTGACGGTCTCCAACGAGGACGACAGTTCCCTCCCCGTATGGACGTTCCGGATGTGGACTATAGGCATGCTCTCGTGCATTCTACTCTCATTCCTCAACACCTTCTTCTCTTACCGCACGGAGCCGCTTGTGATATCGATGATCTCCGTGCAGGTGGCGAGTCTGCCGATCGGACGGTTCATGGCTAGGACGCTGCCGAGGAGAAAGGTTTGGATTCGTGGGAAGTCATACTCGTTGAACCCGGGGGAATTTAACGTAAAAGAGCACGTGCTGATTTCAATATTTGCAAACGCCGGAGCCGGGTTTGGTAGCGGACCGGCGTATGCGGTTAATATTGTGACTATAATCAAAGCGTTTTACGGTAGGAAGATATCCTTCTTTGCAAGTTGGGCACTTGTGATTACCACACAGGTATTGGGGTATGGGTGGGCTGGTATTATCAGAAAGTACGTGGTTGATCCGGGGGCGATGTGGTGGCCTAGCAGTCTCGTTCAGGTTTCTTTGTTCAGGGCTCTGCATGAGAAAGACAACAATCGAATGTCGAGAGGGAAATTCTTTTTGATAGCACTGGTGTGCAGCTTCTCTTGGTACCTAGTGCCGGGCTACCTCTTCACAACTCTCTCAATAATTTCATGGATCTGTTGGATTTACCCCAAGTCCATAAGAGCTCAGCAAATTGGCTCAGGCTTCCGTGGGCTTGGCGTTGGGGCATTTACCCTAGATTGGTCGGTCATATCCTCTTACTTGGGGAGCCCTTTGATCAGCCCCTTCTTTGCCATTGTCAATATCACCGTCGGATACATCATCATCATGTACGTCATCATTCCAATTTCATACTGGAAACTCAACTTGTACAATGCAAGGAATTTTGCAATCTTCTCTTCGCACTTGTTTGATTCCGACGGCCAAATATATAATGTCAAAGGCATTGTGAATGACCGGTTTCAGATTGACATGCCTGCCTATGAAAAACAAGGATTCATAAACTTGAGTGTATTTTTTGCCCTAACATATGGCCTTGGTTTTGCTGCTGTTGTATCGACCCTCACTCATGTGGCTCTCTTCAATGGAAGGGACATATATGAACGATACCAATCGTCATATAGTGGAAAGATAGATATACACACCAAATTAATGAGGAAATACAGGGACATACCCAGCTGGTGGTTTCATATTTTGCTTGTGGTGTCGTTGGTACTTTCTCTAGCATTGTGCATTTTCATGAAAGATGAGGTGCAAATGCCATGGTGGGGACTCATTTTTGCTGCAGGAATTGCATTGATTTTCACCCTTCCAATCAGCATAATAACTGCCACAACAAATGTGACACCGGGACTAAACATCATCACGGAGTACGTCATGGGTTTGATATTGCCTGGCTATCCTATAGCCAATGTATGCTTCAAAACATATGGATACATAAGTATGGCACAGGCAATTTCATTTTTGAGTGATTTTAAGTTAGGCCATTACATGAAGATTCCGCCCATATCCATGTTCTTAGTTCAGTGCGTAGGAACTGTAGTAGCCGGAACCGTCAATATTGGGACAGCATGGTGGCTTCTAACTACAGTGAAAAATATTTGCAATACTGAATTACTCCCTGACACTAGTCCTTGGACTTGTCCTGGTGATAGTGTCTTCTTCAATGCATCTGTGATTTGGGGTCTGGTTGGACCGAAACGAATCTTCGGTTCCCGCGGACTCTACGGAGGACTCAACTGGTTCTTTCTGGTAGGTGCACTCGGACCTGTCATAGTATGGTTGTTTCACAGAGCTTTCCCCAGGCAGAAATGGATCGCCTACATCAACCTTCCAGTGCTTTTCGGATCAACCGCGGTGATGCCACCAGCTACAACTGTGAACTTTAACAGCTGGATTGTGGTCGGAATCGTTTTCAATTACTTTGTTTTCAGATACAGAAAGAAGTGGTGGCAGAGGTACAATTATGTTCTTTCTGCTGCCCTAGATGCTGGAGTGGCTTTTATGGGGGTGCTTATATACATATGTTTAACCATAAACGAGGTAAGCATTTCTTGGTGGGGTACACAAGACTATGACCACTGTAAATTGTCTACTTGTCCAACATCTAAGAGCATAGAACTTGGTCATGCTTGTGTATTCCCTTAA

Protein Analysis

744

Amino Acids

83.56

Weight (kDa)

9.16

Isoelectric Point (pI)

25.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 47 - 704 5.6e-186 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 2 cut(s) 85, 244
Acc65I GGTACC 1 cut(s) 690
AccB1I GGYRCC 2 cut(s) 5, 690
AccB7I CCANNNNNTGG 1 cut(s) 1266
AccI GTMKAC 1 cut(s) 2177
AccII CGCG 2 cut(s) 1803, 1940
AccIII TCCGGA 1 cut(s) 150
AclWI GGATC 4 cut(s) 554, 746, 1907, 1939
AcoI YGGCCR 1 cut(s) 1006
AcsI RAATTY 3 cut(s) 350, 653, 967
AcvI CACGTG 1 cut(s) 370
AfaI GTAC 8 cut(s) 551, 692, 917, 956, 1299, 1457, 2045, 2149
AfiI CCNNNNNNNGG 7 cut(s) 28, 106, 402, 700, 825, 1266, 1352
AflIII ACRYGT 1 cut(s) 78
AgeI ACCGGT 1 cut(s) 1041
AgsI TTSAA 6 cut(s) 340, 381, 1168, 1507, 1750, 2002
AhdI GACNNNNNGTC 1 cut(s) 107
AhlI ACTAGT 1 cut(s) 1712
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 4 cut(s) 9, 1477, 1729, 1886
AjuI GAANNNNNNNTTGG 2 cut(s) 1487, 1519
AleI CACNNNNGTG 2 cut(s) 673, 1154
AluBI AGCT 7 cut(s) 52, 681, 770, 1263, 1880, 1955, 1977
AluI AGCT 7 cut(s) 52, 681, 770, 1263, 1880, 1955, 1977
Alw21I GWGCWC 3 cut(s) 369, 772, 1846
Alw26I GTCTC 2 cut(s) 115, 593
Alw44I GTGCAC 1 cut(s) 1842
AlwI GGATC 4 cut(s) 554, 746, 1907, 1939
Ama87I CYCGRG 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 150
AoxI GGCC 5 cut(s) 33, 578, 1006, 1117, 1567
ApaLI GTGCAC 1 cut(s) 1842
ApeKI GCWGC 6 cut(s) 38, 295, 678, 1130, 1367, 2063
ApoI RAATTY 3 cut(s) 350, 653, 967
AseI ATTAAT 1 cut(s) 1235
AsiGI ACCGGT 1 cut(s) 1041
Asp718I GGTACC 1 cut(s) 690
AspS9I GGNCC 4 cut(s) 97, 416, 1778, 1850
AsuC2I CCSGG 6 cut(s) 344, 345, 403, 564, 702, 1434
AsuHPI GGTGA 5 cut(s) 7, 883, 1381, 1745, 1954
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 4 cut(s) 97, 416, 1778, 1850
BaeGI GKGCMC 3 cut(s) 8, 496, 1846
BaeI ACNNNNGTAYC 2 cut(s) 1510, 1543
BanI GGYRCC 2 cut(s) 5, 690
BanII GRGCYC 3 cut(s) 616, 772, 857
BauI CACGAG 1 cut(s) 175
BbrPI CACGTG 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 1735
Bbv12I GWGCWC 3 cut(s) 369, 772, 1846
BbvI GCAGC 6 cut(s) 25, 282, 690, 1117, 1354, 2050
BceAI ACGGC 1 cut(s) 1021
BciT130I CCWGG 4 cut(s) 11, 1479, 1731, 1888
BciVI GTATCC 2 cut(s) 893, 1511
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 6 cut(s) 344, 345, 403, 564, 702, 1434
BcoDI GTCTC 2 cut(s) 115, 593
BcuI ACTAGT 1 cut(s) 1712
BfaI CTAG 8 cut(s) 23, 288, 582, 695, 821, 1307, 1713, 2069
BfmI CTRYAG 5 cut(s) 162, 1368, 1488, 1623, 1671
BfuAI ACCTGC 2 cut(s) 85, 244
BfuI GTATCC 2 cut(s) 893, 1511
BisI GCNGC 8 cut(s) 36, 39, 227, 296, 679, 1131, 1368, 2064
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 8 cut(s) 37, 40, 228, 297, 680, 1132, 1369, 2065
Bme18I GGWCC 4 cut(s) 97, 416, 1778, 1850
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 343
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 4 cut(s) 97, 416, 1778, 1850
BmiI GGNNCC 7 cut(s) 7, 225, 400, 692, 854, 1636, 1798
BmsI GCATC 3 cut(s) 1763, 1935, 2062
BoxI GACNNNNGTC 1 cut(s) 1713
BpiI GAAGAC 1 cut(s) 1735
BpmI CTGGAG 1 cut(s) 2097
Bpu10I CCTNAGC 1 cut(s) 784
Bpu1102I GCTNAGC 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 1111
BpuMI CCSGG 6 cut(s) 344, 345, 403, 564, 702, 1434
Bsa29I ATCGAT 1 cut(s) 239
BsaAI YACGTR 2 cut(s) 370, 553
BsaBI GATNNNNATC 1 cut(s) 1195
BsaI GGTCTC 1 cut(s) 115
BsaWI WCCGGW 2 cut(s) 150, 1041
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 106, 402, 700, 825, 1266, 1352
Bse118I RCCGGY 2 cut(s) 418, 1041
Bse1I ACTGG 4 cut(s) 675, 944, 1829, 1919
Bse8I GATNNNNATC 1 cut(s) 1195
BseAI TCCGGA 1 cut(s) 150
BseBI CCWGG 4 cut(s) 11, 1479, 1731, 1888
BseCI ATCGAT 1 cut(s) 239
BseGI GGATG 1 cut(s) 159
BseJI GATNNNNATC 1 cut(s) 1195
BseLI CCNNNNNNNGG 7 cut(s) 28, 106, 402, 700, 825, 1266, 1352
BseMII CTCAG 2 cut(s) 785, 798
BseNI ACTGG 4 cut(s) 675, 944, 1829, 1919
BseRI GAGGAG 1 cut(s) 316
BseSI GKGCMC 3 cut(s) 8, 496, 1846
BseXI GCAGC 6 cut(s) 25, 282, 690, 1117, 1354, 2050
BseYI CCCAGC 1 cut(s) 1259
BsgI GTGCAG 2 cut(s) 272, 697
Bsh1236I CGCG 2 cut(s) 1803, 1940
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 5 cut(s) 35, 580, 1008, 1119, 1569
BshNI GGYRCC 2 cut(s) 5, 690
BshTI ACCGGT 1 cut(s) 1041
BshVI ATCGAT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 3 cut(s) 369, 772, 1846
BsiHKCI CYCGRG 1 cut(s) 343
BslFI GGGAC 5 cut(s) 1190, 1265, 1368, 1449, 1663
BslI CCNNNNNNNGG 7 cut(s) 28, 106, 402, 700, 825, 1266, 1352
BsmAI GTCTC 2 cut(s) 115, 593
BsmFI GGGAC 5 cut(s) 1190, 1265, 1368, 1449, 1663
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 5 cut(s) 35, 580, 1008, 1119, 1569
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 7 cut(s) 8, 369, 496, 616, 772, 857, 1846
Bsp13I TCCGGA 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
Bsp1720I GCTNAGC 1 cut(s) 771
Bsp19I CCATGG 1 cut(s) 1346
BspANI GGCC 5 cut(s) 35, 580, 1008, 1119, 1569
BspCNI CTCAG 2 cut(s) 784, 797
BspDI ATCGAT 1 cut(s) 239
BspEI TCCGGA 1 cut(s) 150
BspFNI CGCG 2 cut(s) 1803, 1940
BspHI TCATGA 1 cut(s) 1323
BspLI GGNNCC 7 cut(s) 7, 225, 400, 692, 854, 1636, 1798
BspMAI CTGCAG 1 cut(s) 1372
BspMI ACCTGC 2 cut(s) 85, 244
BspPI GGATC 4 cut(s) 554, 746, 1907, 1939
BspT107I GGYRCC 2 cut(s) 5, 690
BspTNI GGTCTC 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 418, 1041
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 4 cut(s) 675, 944, 1829, 1919
BssAI RCCGGY 2 cut(s) 418, 1041
BssMI GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
BssSI CACGAG 1 cut(s) 175
BssT1I CCWWGG 3 cut(s) 1120, 1346, 1718
Bst2BI CACGAG 1 cut(s) 175
Bst2UI CCWGG 4 cut(s) 11, 1479, 1731, 1888
Bst6I CTCTTC 3 cut(s) 716, 988, 1169
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstAUI TGTACA 1 cut(s) 954
BstBAI YACGTR 2 cut(s) 370, 553
BstC8I GCNNGC 2 cut(s) 170, 1062
BstDEI CTNAG 5 cut(s) 48, 771, 784, 1603, 2193
BstDSI CCRYGG 4 cut(s) 793, 1346, 1801, 1938
BstEII GGTNACC 1 cut(s) 13
BstF5I GGATG 1 cut(s) 159
BstFNI CGCG 2 cut(s) 1803, 1940
BstKTI GATC 7 cut(s) 246, 273, 562, 741, 864, 1902, 1934
BstMAI GTCTC 2 cut(s) 115, 593
BstMBI GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 4 cut(s) 491, 1376, 2072, 2081
BstNI CCWGG 4 cut(s) 11, 1479, 1731, 1888
BstNSI RCATGY 2 cut(s) 172, 1060
BstPAI GACNNNNGTC 1 cut(s) 1713
BstPI GGTNACC 1 cut(s) 13
BstSFI CTRYAG 5 cut(s) 162, 1368, 1488, 1623, 1671
BstSLI GKGCMC 3 cut(s) 8, 496, 1846
BstUI CGCG 2 cut(s) 1803, 1940
BstV1I GCAGC 6 cut(s) 25, 282, 690, 1117, 1354, 2050
BstV2I GAAGAC 1 cut(s) 1735
BstX2I RGATCY 1 cut(s) 738
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 239
BsuI GTATCC 2 cut(s) 893, 1511
BsuRI GGCC 5 cut(s) 35, 580, 1008, 1119, 1569
BsuTUI ATCGAT 1 cut(s) 239
BtgI CCRYGG 4 cut(s) 793, 1346, 1801, 1938
BtgZI GCGATG 1 cut(s) 584
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 159
BtsIMutI CAGTG 5 cut(s) 668, 1616, 1680, 1926, 2164
BveI ACCTGC 2 cut(s) 85, 244
Cac8I GCNNGC 2 cut(s) 170, 1062
CciI TCATGA 1 cut(s) 1323
Cfr10I RCCGGY 2 cut(s) 418, 1041
Cfr13I GGNCC 4 cut(s) 97, 416, 1778, 1850
Cfr42I CCGCGG 2 cut(s) 1804, 1941
Cfr9I CCCGGG 1 cut(s) 343
ClaI ATCGAT 1 cut(s) 239
CpoI CGGWCCG 1 cut(s) 416
CseI GACGC 1 cut(s) 301
Csp6I GTAC 8 cut(s) 550, 691, 916, 955, 1298, 1456, 2044, 2148
CspAI ACCGGT 1 cut(s) 1041
CspI CGGWCCG 1 cut(s) 416
CviQI GTAC 8 cut(s) 550, 691, 916, 955, 1298, 1456, 2044, 2148
DdeI CTNAG 5 cut(s) 48, 771, 784, 1603, 2193
DpnI GATC 7 cut(s) 245, 272, 561, 740, 863, 1901, 1933
DpnII GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
DriI GACNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 1006
Eam1104I CTCTTC 3 cut(s) 716, 988, 1169
Eam1105I GACNNNNNGTC 1 cut(s) 107
EarI CTCTTC 3 cut(s) 716, 988, 1169
EciI GGCGGA 1 cut(s) 1575
Ecl136II GAGCTC 1 cut(s) 770
Eco130I CCWWGG 3 cut(s) 1120, 1346, 1718
Eco24I GRGCYC 3 cut(s) 616, 772, 857
Eco31I GGTCTC 1 cut(s) 115
Eco32I GATATC 2 cut(s) 237, 474
Eco47I GGWCC 4 cut(s) 97, 416, 1778, 1850
Eco53kI GAGCTC 1 cut(s) 770
Eco72I CACGTG 1 cut(s) 370
Eco88I CYCGRG 1 cut(s) 343
Eco91I GGTNACC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 770
EcoO65I GGTNACC 1 cut(s) 13
EcoRII CCWGG 4 cut(s) 9, 1477, 1729, 1886
EcoRV GATATC 2 cut(s) 237, 474
EcoT14I CCWWGG 3 cut(s) 1120, 1346, 1718
EcoT22I ATGCAT 1 cut(s) 1756
EcoT38I GRGCYC 3 cut(s) 616, 772, 857
ErhI CCWWGG 3 cut(s) 1120, 1346, 1718
FalI AAGNNNNNCTT 4 cut(s) 461, 493, 2122, 2154
FaqI GGGAC 5 cut(s) 1190, 1265, 1368, 1449, 1663
FauI CCCGC 1 cut(s) 1808
FauNDI CATATG 3 cut(s) 1114, 1513, 2108
FbaI TGATCA 1 cut(s) 861
FblI GTMKAC 1 cut(s) 2177
Fnu4HI GCNGC 8 cut(s) 36, 39, 227, 296, 679, 1131, 1368, 2064
FokI GGATG 1 cut(s) 166
FriOI GRGCYC 3 cut(s) 616, 772, 857
Fsp4HI GCNGC 8 cut(s) 36, 39, 227, 296, 679, 1131, 1368, 2064
FspBI CTAG 8 cut(s) 23, 288, 582, 695, 821, 1307, 1713, 2069
GluI GCNGC 8 cut(s) 36, 39, 227, 296, 679, 1131, 1368, 2064
GsaI CCCAGC 1 cut(s) 1263
GsuI CTGGAG 1 cut(s) 2097
HaeIII GGCC 5 cut(s) 35, 580, 1008, 1119, 1569
HgaI GACGC 1 cut(s) 301
HphI GGTGA 5 cut(s) 7, 883, 1381, 1745, 1954
Hpy166II GTNNAC 5 cut(s) 159, 1844, 1966, 2150, 2178
Hpy188III TCNNGA 3 cut(s) 151, 645, 1324
Hpy8I GTNNAC 5 cut(s) 159, 1844, 1966, 2150, 2178
Hpy99I CGWCG 3 cut(s) 74, 899, 1007
HpyAV CCTTC 6 cut(s) 214, 487, 880, 1167, 1403, 1925
HpyCH4IV ACGT 7 cut(s) 78, 146, 357, 369, 552, 918, 1458
HpyF10VI GCNNNNNNNGC 4 cut(s) 491, 1376, 2072, 2081
HpyF3I CTNAG 5 cut(s) 48, 771, 784, 1603, 2193
HpySE526I ACGT 7 cut(s) 78, 146, 357, 369, 552, 918, 1458
Kpn2I TCCGGA 1 cut(s) 150
KpnI GGTACC 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 861
KspI CCGCGG 2 cut(s) 1804, 1941
Kzo9I GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
LmnI GCTCC 3 cut(s) 223, 398, 852
Lsp1109I GCAGC 6 cut(s) 25, 282, 690, 1117, 1354, 2050
LweI GCATC 3 cut(s) 1763, 1935, 2062
MaeI CTAG 8 cut(s) 23, 288, 582, 695, 821, 1307, 1713, 2069
MaeII ACGT 7 cut(s) 78, 146, 357, 369, 552, 918, 1458
MaeIII GTNAC 3 cut(s) 13, 439, 1426
MalI GATC 7 cut(s) 245, 272, 561, 740, 863, 1901, 1933
MboI GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 7 cut(s) 8, 369, 496, 616, 772, 857, 1846
MlyI GAGTC 4 cut(s) 271, 1350, 1800, 1812
MmeI TCCRAC 8 cut(s) 124, 138, 725, 877, 1026, 1756, 1969, 2210
Mph1103I ATGCAT 1 cut(s) 1756
MroI TCCGGA 1 cut(s) 150
MseI TTAA 7 cut(s) 354, 432, 1235, 1560, 1971, 2114, 2230
MslI CAYNNNNRTG 7 cut(s) 673, 911, 1154, 1322, 1526, 1595, 2217
MspA1I CMGCKG 5 cut(s) 38, 1263, 1803, 1940, 1977
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 4 cut(s) 11, 1479, 1731, 1888
MvnI CGCG 2 cut(s) 1803, 1940
MwoI GCNNNNNNNGC 4 cut(s) 491, 1376, 2072, 2081
NciI CCSGG 6 cut(s) 344, 345, 403, 564, 702, 1434
NcoI CCATGG 1 cut(s) 1346
NdeI CATATG 3 cut(s) 1114, 1513, 2108
NdeII GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
NlaIV GGNNCC 7 cut(s) 7, 225, 400, 692, 854, 1636, 1798
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 3 cut(s) 13, 439, 1426
NsiI ATGCAT 1 cut(s) 1756
NspI RCATGY 2 cut(s) 172, 1060
OliI CACNNNNGTG 2 cut(s) 673, 1154
PaeI GCATGC 1 cut(s) 172
PagI TCATGA 1 cut(s) 1323
PaqCI CACCTGC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 6 cut(s) 316, 998, 1080, 1582, 1788, 1992
PflMI CCANNNNNTGG 1 cut(s) 1266
PinAI ACCGGT 1 cut(s) 1041
PkrI GCNGC 8 cut(s) 37, 40, 228, 297, 680, 1132, 1369, 2065
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 4 cut(s) 270, 1350, 1800, 1812
PmaCI CACGTG 1 cut(s) 370
PmlI CACGTG 1 cut(s) 370
PpsI GAGTC 4 cut(s) 270, 1350, 1800, 1812
Ppu21I YACGTR 2 cut(s) 370, 553
PshAI GACNNNNGTC 1 cut(s) 1713
PshBI ATTAAT 1 cut(s) 1235
Psp124BI GAGCTC 1 cut(s) 772
Psp6I CCWGG 4 cut(s) 9, 1477, 1729, 1886
PspCI CACGTG 1 cut(s) 370
PspEI GGTNACC 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 1259
PspGI CCWGG 4 cut(s) 9, 1477, 1729, 1886
PspN4I GGNNCC 7 cut(s) 7, 225, 400, 692, 854, 1636, 1798
PspPI GGNCC 4 cut(s) 97, 416, 1778, 1850
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 1 cut(s) 738
PvuI CGATCG 1 cut(s) 273
PvuII CAGCTG 2 cut(s) 1263, 1977
RsaI GTAC 8 cut(s) 551, 692, 917, 956, 1299, 1457, 2045, 2149
RsaNI GTAC 8 cut(s) 550, 691, 916, 955, 1298, 1456, 2044, 2148
RseI CAYNNNNRTG 7 cut(s) 673, 911, 1154, 1322, 1526, 1595, 2217
Rsr2I CGGWCCG 1 cut(s) 416
RsrII CGGWCCG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 772
SacII CCGCGG 2 cut(s) 1804, 1941
SaqAI TTAA 7 cut(s) 354, 432, 1235, 1560, 1971, 2114, 2230
SatI GCNGC 8 cut(s) 36, 39, 227, 296, 679, 1131, 1368, 2064
Sau3AI GATC 7 cut(s) 243, 270, 559, 738, 861, 1899, 1931
Sau96I GGNCC 4 cut(s) 97, 416, 1778, 1850
SchI GAGTC 4 cut(s) 271, 1350, 1800, 1812
SduI GDGCHC 7 cut(s) 8, 369, 496, 616, 772, 857, 1846
SfaNI GCATC 3 cut(s) 1763, 1935, 2062
SfcI CTRYAG 5 cut(s) 162, 1368, 1488, 1623, 1671
Sfr303I CCGCGG 2 cut(s) 1804, 1941
SgrBI CCGCGG 2 cut(s) 1804, 1941
SinI GGWCC 4 cut(s) 97, 416, 1778, 1850
SmaI CCCGGG 1 cut(s) 345
SmiMI CAYNNNNRTG 7 cut(s) 673, 911, 1154, 1322, 1526, 1595, 2217
SmlI CTYRAG 1 cut(s) 1090
SmoI CTYRAG 1 cut(s) 1090
SpeI ACTAGT 1 cut(s) 1712
SphI GCATGC 1 cut(s) 172
SspI AATATT 4 cut(s) 384, 436, 1645, 1684
SspMI CTAG 8 cut(s) 23, 288, 582, 695, 821, 1307, 1713, 2069
SstI GAGCTC 1 cut(s) 772
StyI CCWWGG 3 cut(s) 1120, 1346, 1718
TaiI ACGT 7 cut(s) 81, 149, 360, 372, 555, 921, 1461
TaqI TCGA 5 cut(s) 54, 239, 637, 644, 1142
TaqII GACCGA 2 cut(s) 820, 1795
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 38, 229
TfiI GAWTC 6 cut(s) 316, 998, 1080, 1582, 1788, 1992
Tru1I TTAA 7 cut(s) 354, 432, 1235, 1560, 1971, 2114, 2230
Tru9I TTAA 7 cut(s) 354, 432, 1235, 1560, 1971, 2114, 2230
TscAI CASTG 5 cut(s) 675, 1616, 1680, 1926, 2171
TseFI GTSAC 3 cut(s) 13, 439, 1426
TseI GCWGC 6 cut(s) 38, 295, 678, 1130, 1367, 2063
Tsp45I GTSAC 3 cut(s) 13, 439, 1426
TspGWI ACGGA 5 cut(s) 236, 238, 782, 1466, 1827
TspMI CCCGGG 1 cut(s) 343
TspRI CASTG 5 cut(s) 675, 1616, 1680, 1926, 2171
Van91I CCANNNNNTGG 1 cut(s) 1266
VneI GTGCAC 1 cut(s) 1842
VpaK11BI GGWCC 4 cut(s) 97, 416, 1778, 1850
VspI ATTAAT 1 cut(s) 1235
XapI RAATTY 3 cut(s) 350, 653, 967
XceI RCATGY 2 cut(s) 172, 1060
XcmI CCANNNNNNNNNTGG 3 cut(s) 25, 514, 936
XmaI CCCGGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 2177
XspI CTAG 8 cut(s) 23, 288, 582, 695, 821, 1307, 1713, 2069
Zsp2I ATGCAT 1 cut(s) 1756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.