MD08G1248300.v1.1

Survival of motor neuron-related-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002626
Physical Location & Seq
Reverse (-)
31235245 .. 31241482
6238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1248300.v1.1.491

Sequence Viewer

Length: 915 bp
ATGGAAGGAGAAGGAGGAGAAGAGCTCAGCATACAGGAGCTGGCCTCCAATCTCTCAACCTACAAAGATCAGCTTCACCAGGTAAAGGAGCTTTTAGCTGATGATCCTGCAAACTCTGAGTATGTTGACATGGAAAGAGAGCTCAATGAGGTAATTGCTCTGACAGAAGAACTACTTGCAACTGCAAAGCAAAATCAGAATTCCGGATTAGATTTCGGGACAAGTGATAGTACATCACCTAGTTTGCCCCAGACTCAGAGGAATGAAAATGATTCAGGGAATATTTCCGATTATCATGACAAGTTTTCTGTGGGCACAAAAGTTCAAGCTGTTTGGAGTGAAGATGGGGAGTGGTATGATGCCACAATCGAGTCTCTTACTCCAAATGGGTATTTCGTTACTTATGATAGCTGGGGGAATAAAGAAGAGGTGGATCCAGACAATGTAAGACCAATTCAAGAAGGGGCTGTCAATGCATTGCTGGAAGCTGAAAGGGTTGCTGAGGCCACAAAACAAGCACTCAAAAGGAAGATTGCACAAGCTTCTTCTATTGACTTCCAATCACGAAGTTTACCACCAAAACTTCGTATAGAACCTGATGATCCAGAGGATGTGAAAGTTACCAAACGCAAAAAGATACATGCTTTCAAGTCGAAGATGAGGATGGAACAACTGGAAGTTACACAGAATAAACGTCAAAATGCTTGGCAACAGTTCCAAACAACCAAAGGCAAGACTAAGAAGATTGGTTTCTTTTCTGGACGCAAGCGTGAGAGCATTTTCAAGTCTCCTGATGATCCTTTTGGGAAGGTTGGGGTGACTGGAAGTGGGAAGGGCTTGACAGATTTTCAGAAGAGGGAAAAACACTTGCACCTCAAAGGGGGAACGGGTGAGAATGATGAAGAGGCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SMN

Protein Analysis

305

Amino Acids

34.07

Weight (kDa)

5.09

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_Tudor PF06003 101 - 144 5.4e-06 Survival motor neuron protein (SMN), Tudor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 203
AclWI GGATC 5 cut(s) 98, 428, 441, 596, 791
AcsI RAATTY 1 cut(s) 199
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 2 cut(s) 85, 880
AgsI TTSAA 4 cut(s) 326, 458, 649, 784
AjnI CCWGG 1 cut(s) 78
Alw21I GWGCWC 2 cut(s) 27, 144
Alw26I GTCTC 2 cut(s) 378, 792
AlwI GGATC 5 cut(s) 98, 428, 441, 596, 791
AlwNI CAGNNNCTG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 2 cut(s) 42, 504
ApoI RAATTY 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 68, 228, 829, 902
BaeGI GKGCMC 1 cut(s) 317
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 27, 144
Bbv12I GWGCWC 2 cut(s) 27, 144
BbvCI CCTCAGC 1 cut(s) 501
BccI CCATC 2 cut(s) 338, 658
BciT130I CCWGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 378, 792
BfaI CTAG 1 cut(s) 240
BlpI GCTNAGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 435, 909
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 349
BplI GAGNNNNNCTC 4 cut(s) 9, 41, 29, 61
Bpu10I CCTNAGC 1 cut(s) 501
Bpu1102I GCTNAGC 1 cut(s) 26
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 880
Bse1I ACTGG 2 cut(s) 678, 826
Bse3DI GCAATG 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 1 cut(s) 80
BseGI GGATG 2 cut(s) 616, 669
BseLI CCNNNNNNNGG 2 cut(s) 85, 880
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 4 cut(s) 40, 108, 269, 492
BseNI ACTGG 2 cut(s) 678, 826
BseRI GAGGAG 1 cut(s) 30
BseSI GKGCMC 1 cut(s) 317
BseYI CCCAGC 1 cut(s) 411
BshFI GGCC 2 cut(s) 44, 506
BsiHKAI GWGCWC 2 cut(s) 27, 144
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 85, 880
BsmAI GTCTC 2 cut(s) 378, 792
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 44, 506
Bsp1286I GDGCHC 3 cut(s) 27, 144, 317
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 5 cut(s) 67, 103, 433, 601, 796
Bsp1720I GCTNAGC 1 cut(s) 26
BspANI GGCC 2 cut(s) 44, 506
BspCNI CTCAG 4 cut(s) 39, 109, 268, 493
BspEI TCCGGA 1 cut(s) 203
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 435, 909
BspPI GGATC 5 cut(s) 98, 428, 441, 596, 791
BspQI GCTCTTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 476
BsrI ACTGG 2 cut(s) 678, 826
BssMI GATC 5 cut(s) 67, 103, 433, 601, 796
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 714
Bst6I CTCTTC 4 cut(s) 15, 420, 848, 897
BstC8I GCNNGC 2 cut(s) 42, 767
BstDEI CTNAG 6 cut(s) 26, 117, 255, 501, 738, 912
BstF5I GGATG 2 cut(s) 616, 669
BstKTI GATC 5 cut(s) 70, 106, 436, 604, 799
BstMAI GTCTC 2 cut(s) 378, 792
BstMBI GATC 5 cut(s) 67, 103, 433, 601, 796
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 44, 506
BtsCI GGATG 2 cut(s) 616, 669
Cac8I GCNNGC 2 cut(s) 42, 767
CaiI CAGNNNCTG 1 cut(s) 40
CciI TCATGA 1 cut(s) 295
CseI GACGC 1 cut(s) 771
CsiI ACCWGGT 1 cut(s) 78
Csp6I GTAC 1 cut(s) 231
CviAII CATG 3 cut(s) 130, 296, 641
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 6 cut(s) 26, 117, 255, 501, 738, 912
DpnI GATC 5 cut(s) 69, 105, 435, 603, 798
DpnII GATC 5 cut(s) 67, 103, 433, 601, 796
Eam1104I CTCTTC 4 cut(s) 15, 420, 848, 897
EarI CTCTTC 4 cut(s) 15, 420, 848, 897
Ecl136II GAGCTC 2 cut(s) 25, 142
Eco24I GRGCYC 2 cut(s) 27, 144
Eco53kI GAGCTC 2 cut(s) 25, 142
EcoICRI GAGCTC 2 cut(s) 25, 142
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 478
EcoT38I GRGCYC 2 cut(s) 27, 144
FaeI CATG 3 cut(s) 133, 299, 644
FaiI YATR 8 cut(s) 32, 123, 131, 297, 357, 405, 590, 642
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 159, 191
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 129, 295, 640
FokI GGATG 2 cut(s) 623, 676
FriOI GRGCYC 2 cut(s) 27, 144
FspBI CTAG 1 cut(s) 240
GsaI CCCAGC 1 cut(s) 415
HaeIII GGCC 2 cut(s) 44, 506
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 771
Hin1II CATG 3 cut(s) 133, 299, 644
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 3 cut(s) 253, 272, 371
HpaII CCGG 1 cut(s) 204
HphI GGTGA 4 cut(s) 68, 228, 829, 902
Hpy166II GTNNAC 2 cut(s) 127, 572
Hpy188I TCNGA 6 cut(s) 118, 162, 198, 258, 289, 852
Hpy188III TCNNGA 9 cut(s) 204, 217, 296, 437, 458, 564, 605, 759, 791
Hpy8I GTNNAC 2 cut(s) 127, 572
HpyAV CCTTC 4 cut(s) 5, 455, 802, 826
HpyCH4III ACNGT 1 cut(s) 714
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 6 cut(s) 110, 179, 185, 476, 536, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 6 cut(s) 26, 117, 255, 501, 738, 912
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 3 cut(s) 133, 299, 644
Kpn2I TCCGGA 1 cut(s) 203
Kzo9I GATC 5 cut(s) 67, 103, 433, 601, 796
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 37, 88, 913
LweI GCATC 1 cut(s) 349
MabI ACCWGGT 1 cut(s) 78
MaeI CTAG 1 cut(s) 240
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 4 cut(s) 397, 619, 679, 817
MalI GATC 5 cut(s) 69, 105, 435, 603, 798
MboI GATC 5 cut(s) 67, 103, 433, 601, 796
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 3 cut(s) 27, 144, 317
MluCI AATT 3 cut(s) 153, 199, 453
MlyI GAGTC 2 cut(s) 247, 380
Mph1103I ATGCAT 1 cut(s) 478
MroI TCCGGA 1 cut(s) 203
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 473
NdeII GATC 5 cut(s) 67, 103, 433, 601, 796
NlaIII CATG 3 cut(s) 133, 299, 644
NlaIV GGNNCC 2 cut(s) 435, 909
NmuCI GTSAC 1 cut(s) 817
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 1 cut(s) 644
PagI TCATGA 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 272
PleI GAGTC 2 cut(s) 247, 379
PpsI GAGTC 2 cut(s) 247, 379
Psp124BI GAGCTC 2 cut(s) 27, 144
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 435, 909
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SacI GAGCTC 2 cut(s) 27, 144
SapI GCTCTTC 1 cut(s) 15
Sau3AI GATC 5 cut(s) 67, 103, 433, 601, 796
SchI GAGTC 2 cut(s) 247, 380
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 3 cut(s) 27, 144, 317
SexAI ACCWGGT 1 cut(s) 78
SfaNI GCATC 1 cut(s) 349
Sse9I AATT 3 cut(s) 153, 199, 453
SspI AATATT 1 cut(s) 283
SspMI CTAG 1 cut(s) 240
SstI GAGCTC 2 cut(s) 27, 144
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 714
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 369, 653
TasI AATT 3 cut(s) 153, 199, 453
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 1 cut(s) 272
TseFI GTSAC 1 cut(s) 817
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 2 cut(s) 279, 915
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 644
XspI CTAG 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.