Rroxscaffold_3G00239950

Survival of motor neuron-related-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002626
Physical Location & Seq
Forward (+)
31289836 .. 31295747
5912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239950.1

Sequence Viewer

Length: 1023 bp
ATGCAAGGGGAAGGAGGAGAAGAGCTAACCATAGAGGAGCTCGCCTCCAATCTCTCCACATACAGAGAGCAGCTTCAACAGGTCAAGGAGGTTTTAGCCCATGACCCTGGAAACTCTGAGTATGCTGATGTGGAAAGAGAGCTCCATGAGAGTCAACATCTTCTCTGGCCCTTAGGGTTGATTCCCAAGAGCAGTTACTATTATATGGATAGGTATGCTATGTGTGGCGTTGATCAGATTCTCTTTCTCTGTCACCTGGTAATAGCCATGACAGAAGAGCTACTTGCAACTGCCAAGCAAAATCAGAATTTTGGATTAGAAATTGGAACAAGGGGTAGTGCGTCTCCTAGTTTTCCCCAATCTCAGAAGAGTGAAAACGATGCAGGAAGCATTTCTGATTATCATGACAAATTTGCTCCTGGTACAAAAGTTCAAGCTGTTTGGAGTGAAGATGGGGAGTGGTATGAAGCAACAATTGATTCTCTTACTCCAAATGGGTATTATGTTACATATGATGGCTGGGGGAATAAAGAAGAGGTGGATCCAGCCAATGTAAGGCCAATTCAAGAAGGGGTTGTCAATGCATTGTTGGAAGCGGAAAAAGTTGCTGAAGCTACAAAACAAGCAATCAAAAGGAAAATTGCACAAGCTGCTTCTGTTGACTTCCAATCACGAAGCCTACCGGCAAAGCTTCGTATAGAACCGGATGATCCTGAGGATGTGAAAATTGCAAAACGCAAGAAGATACATGCTTTTAAGTCAAAAATGAGGATGGAGCAGTTGGAAGTCACTCAGAACAAGCGTCAAAATGCTTGGCAGCAGTTCCAAACTACCAAAGGCAAGACTAAGAAGAGTGGTTATTTATCTGGACGCAAGCGGGAGAGCATCTTCAAGTCTCCTGACGATCCTTTTGGAAAGGTTGGGGTGACTGGCAGTGGGAAGGGTTTGACAGACTTTCAAAAGAGAGAAAAGCATTTGCATCTCAAAGGGGGAGCAGCTGAGAATGATGATGAGGCTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SMN

Protein Analysis

340

Amino Acids

38.07

Weight (kDa)

5.63

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_Tudor PF06003 140 - 186 3e-06 Survival motor neuron protein (SMN), Tudor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 596, 877
AclWI GGATC 4 cut(s) 536, 549, 704, 899
AcsI RAATTY 2 cut(s) 307, 410
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 555
AgsI TTSAA 5 cut(s) 77, 434, 566, 892, 959
AjnI CCWGG 3 cut(s) 106, 255, 418
Alw21I GWGCWC 2 cut(s) 42, 144
Alw26I GTCTC 2 cut(s) 348, 900
AlwI GGATC 4 cut(s) 536, 549, 704, 899
AoxI GGCC 2 cut(s) 167, 557
ApeKI GCWGC 4 cut(s) 70, 650, 817, 995
ApoI RAATTY 2 cut(s) 307, 410
ArsI GACNNNNNNTTYG 2 cut(s) 893, 925
Asp700I GAANNNNTTC 1 cut(s) 391
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 245, 937
AxyI CCTNAGG 2 cut(s) 172, 714
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 42, 144
Bbv12I GWGCWC 2 cut(s) 42, 144
BbvI GCAGC 4 cut(s) 82, 637, 829, 1007
BccI CCATC 3 cut(s) 446, 509, 766
BciT130I CCWGG 3 cut(s) 108, 257, 420
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 348, 900
BfaI CTAG 1 cut(s) 348
BisI GCNGC 4 cut(s) 71, 651, 818, 996
BlsI GCNGC 4 cut(s) 72, 652, 819, 997
Bme1390I CCNGG 3 cut(s) 108, 257, 420
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 543, 1017
BmrFI CCNGG 3 cut(s) 108, 257, 420
BmsI GCATC 3 cut(s) 370, 894, 988
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse118I RCCGGY 1 cut(s) 682
Bse1I ACTGG 1 cut(s) 934
Bse21I CCTNAGG 2 cut(s) 172, 714
BseBI CCWGG 3 cut(s) 108, 257, 420
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 3 cut(s) 712, 724, 777
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMII CTCAG 5 cut(s) 108, 377, 705, 806, 990
BseNI ACTGG 1 cut(s) 934
BseRI GAGGAG 2 cut(s) 30, 50
BseXI GCAGC 4 cut(s) 82, 637, 829, 1007
BseYI CCCAGC 1 cut(s) 519
BshFI GGCC 2 cut(s) 169, 559
BsiHKAI GWGCWC 2 cut(s) 42, 144
BsiSI CCGG 2 cut(s) 683, 704
BslI CCNNNNNNNGG 1 cut(s) 555
BsmAI GTCTC 2 cut(s) 348, 900
BsmBI CGTCTC 1 cut(s) 348
BsnI GGCC 2 cut(s) 169, 559
Bsp1286I GDGCHC 2 cut(s) 42, 144
Bsp143I GATC 4 cut(s) 232, 541, 709, 904
BspACI CCGC 2 cut(s) 596, 877
BspANI GGCC 2 cut(s) 169, 559
BspCNI CTCAG 5 cut(s) 109, 376, 706, 805, 991
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 543, 1017
BspPI GGATC 4 cut(s) 536, 549, 704, 899
BspQI GCTCTTC 2 cut(s) 15, 270
BsrFI RCCGGY 1 cut(s) 682
BsrI ACTGG 1 cut(s) 934
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 4 cut(s) 232, 541, 709, 904
Bst2UI CCWGG 3 cut(s) 108, 257, 420
Bst6I CTCTTC 5 cut(s) 15, 270, 362, 528, 845
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 42, 875
BstDEI CTNAG 7 cut(s) 117, 172, 363, 714, 792, 846, 999
BstF5I GGATG 3 cut(s) 712, 724, 777
BstKTI GATC 4 cut(s) 235, 544, 712, 907
BstMAI GTCTC 2 cut(s) 348, 900
BstMBI GATC 4 cut(s) 232, 541, 709, 904
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 3 cut(s) 108, 257, 420
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 3 cut(s) 106, 255, 418
BstV1I GCAGC 4 cut(s) 82, 637, 829, 1007
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 541
Bsu36I CCTNAGG 2 cut(s) 172, 714
BsuRI GGCC 2 cut(s) 169, 559
BtsCI GGATG 3 cut(s) 712, 724, 777
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 1 cut(s) 940
Cac8I GCNNGC 2 cut(s) 42, 875
CciI TCATGA 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 1 cut(s) 168
CseI GACGC 3 cut(s) 330, 791, 879
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 1 cut(s) 423
CviAII CATG 5 cut(s) 101, 146, 268, 404, 749
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 7 cut(s) 117, 172, 363, 714, 792, 846, 999
DpnI GATC 4 cut(s) 234, 543, 711, 906
DpnII GATC 4 cut(s) 232, 541, 709, 904
Eam1104I CTCTTC 5 cut(s) 15, 270, 362, 528, 845
EarI CTCTTC 5 cut(s) 15, 270, 362, 528, 845
Ecl136II GAGCTC 2 cut(s) 40, 142
Eco24I GRGCYC 2 cut(s) 42, 144
Eco53kI GAGCTC 2 cut(s) 40, 142
Eco57I CTGAAG 1 cut(s) 630
Eco81I CCTNAGG 2 cut(s) 172, 714
EcoICRI GAGCTC 2 cut(s) 40, 142
EcoRII CCWGG 3 cut(s) 106, 255, 418
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 2 cut(s) 42, 144
Esp3I CGTCTC 1 cut(s) 348
FaeI CATG 5 cut(s) 104, 149, 271, 407, 752
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FatI CATG 5 cut(s) 100, 145, 267, 403, 748
FauI CCCGC 1 cut(s) 870
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 4 cut(s) 71, 651, 818, 996
FokI GGATG 3 cut(s) 719, 731, 784
FriOI GRGCYC 2 cut(s) 42, 144
Fsp4HI GCNGC 4 cut(s) 71, 651, 818, 996
FspBI CTAG 1 cut(s) 348
GluI GCNGC 4 cut(s) 71, 651, 818, 996
GsaI CCCAGC 1 cut(s) 523
HaeIII GGCC 2 cut(s) 169, 559
HapII CCGG 2 cut(s) 683, 704
HgaI GACGC 3 cut(s) 330, 791, 879
Hin1II CATG 5 cut(s) 104, 149, 271, 407, 752
HincII GTYRAC 2 cut(s) 155, 661
HindII GTYRAC 2 cut(s) 155, 661
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 151, 181, 238, 479
HpaII CCGG 2 cut(s) 683, 704
HphI GGTGA 2 cut(s) 245, 937
Hpy166II GTNNAC 2 cut(s) 155, 661
Hpy188I TCNGA 6 cut(s) 118, 237, 306, 366, 397, 795
Hpy188III TCNNGA 6 cut(s) 404, 566, 672, 713, 867, 899
Hpy8I GTNNAC 2 cut(s) 155, 661
HpyAV CCTTC 3 cut(s) 5, 563, 934
HpyCH4V TGCA 7 cut(s) 4, 287, 383, 584, 644, 731, 979
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 7 cut(s) 117, 172, 363, 714, 792, 846, 999
Hsp92II CATG 5 cut(s) 104, 149, 271, 407, 752
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 4 cut(s) 232, 541, 709, 904
LguI GCTCTTC 2 cut(s) 15, 270
LmnI GCTCC 6 cut(s) 37, 147, 421, 775, 992, 1021
Lsp1109I GCAGC 4 cut(s) 82, 637, 829, 1007
LweI GCATC 3 cut(s) 370, 894, 988
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 5 cut(s) 194, 251, 505, 787, 925
MalI GATC 4 cut(s) 234, 543, 711, 906
MboI GATC 4 cut(s) 232, 541, 709, 904
MboII GAAGA 9 cut(s) 32, 152, 287, 379, 461, 545, 754, 862, 880
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 42, 144
MluCI AATT 7 cut(s) 307, 321, 410, 474, 561, 639, 726
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 2 cut(s) 570, 762
MnlI CCTC 8 cut(s) 8, 28, 55, 82, 529, 709, 762, 1006
Mph1103I ATGCAT 1 cut(s) 586
MroXI GAANNNNTTC 1 cut(s) 391
MseI TTAA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 2 cut(s) 683, 704
MspR9I CCNGG 3 cut(s) 108, 257, 420
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 3 cut(s) 108, 257, 420
MwoI GCNNNNNNNGC 1 cut(s) 650
NdeI CATATG 1 cut(s) 511
NdeII GATC 4 cut(s) 232, 541, 709, 904
NlaIII CATG 5 cut(s) 104, 149, 271, 407, 752
NlaIV GGNNCC 2 cut(s) 543, 1017
NmuCI GTSAC 3 cut(s) 251, 787, 925
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 752
PagI TCATGA 1 cut(s) 403
PciSI GCTCTTC 2 cut(s) 15, 270
PdmI GAANNNNTTC 1 cut(s) 391
PfeI GAWTC 3 cut(s) 181, 238, 479
PkrI GCNGC 4 cut(s) 72, 652, 819, 997
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 2 cut(s) 42, 144
Psp6I CCWGG 3 cut(s) 106, 255, 418
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 3 cut(s) 106, 255, 418
PspN4I GGNNCC 2 cut(s) 543, 1017
PspPI GGNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 2 cut(s) 42, 144
SapI GCTCTTC 2 cut(s) 15, 270
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 4 cut(s) 71, 651, 818, 996
Sau3AI GATC 4 cut(s) 232, 541, 709, 904
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 3 cut(s) 108, 257, 420
SduI GDGCHC 2 cut(s) 42, 144
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 3 cut(s) 370, 894, 988
Sse9I AATT 7 cut(s) 307, 321, 410, 474, 561, 639, 726
SsiI CCGC 2 cut(s) 596, 877
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 2 cut(s) 42, 144
StyD4I CCNGG 3 cut(s) 106, 255, 418
TasI AATT 7 cut(s) 307, 321, 410, 474, 561, 639, 726
TfiI GAWTC 3 cut(s) 181, 238, 479
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 3 cut(s) 251, 787, 925
TseI GCWGC 4 cut(s) 70, 650, 817, 995
Tsp45I GTSAC 3 cut(s) 251, 787, 925
TspDTI ATGAA 1 cut(s) 480
TspGWI ACGGA 1 cut(s) 1008
TspRI CASTG 1 cut(s) 940
XapI RAATTY 2 cut(s) 307, 410
XceI RCATGY 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 391
XspI CTAG 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.