MD09G1170400.v1.1

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005233
Physical Location & Seq
Forward (+)
14288710 .. 14289918
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1170400.v1.1.491

Sequence Viewer

Length: 618 bp
ATGACGGGACAAGTGGAGGGTATGGGAGGGCACAGGAAGGAAGTGGGAGGGCAGAGAATCTGCCTCCGAAAGAAAGGTGCTGCACACGTTAAGTCCATTGACAGTCAGCATTTACTAGAGATAGGGCTTGAAGGATTTTACGGGCAAACAACGCCGGACAAGATGCAGTTCACTCCTGGTAACGAAGAGTATGGATCTGAATTCACTGCCAGCAACTTGCTTCCCCAGATCGATCTCGCCACTATACATATCTACGCTGAGCAATGGTTATCGGGGTCAAGCGAAGAGGCTCAAGAGGCTTTTGTAGACAAATGGGTGCAGGAACACATTCAAGACAGCAATACAGTAGTTAAGAAACCACTGATTTTGGGAGAATTTGGCAAGTCTTATAAATTACCAGGGTACAGCATAGAAAAGAGGAACACCTACATTGCTAAACTATACAATGGCATATACAACAGTGCCAGTACCGGTGGCTCATGTGTGGGTGGGCTTTTCTGGCAGCTGATGGCTCAAGGAATGGAGAATTTTGGGGATGGATACGAAGTCGTTTTGGAGGAGAGCCCTACTACTGCCAATGTCATTGATCGGCAGTCCAGGAAGCTTGGTGGGTTATAG

Protein Analysis

206

Amino Acids

22.57

Weight (kDa)

5.41

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 25 - 168 9.8e-08 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AccI GTMKAC 1 cut(s) 306
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 3 cut(s) 200, 374, 526
AfaI GTAC 2 cut(s) 404, 469
AflIII ACRYGT 1 cut(s) 85
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 2 cut(s) 131, 332
AjnI CCWGG 3 cut(s) 175, 397, 596
AluBI AGCT 2 cut(s) 505, 604
AluI AGCT 2 cut(s) 505, 604
AlwI GGATC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 80, 502
ApoI RAATTY 3 cut(s) 200, 374, 526
AsiGI ACCGGT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 566
BbvI GCAGC 2 cut(s) 67, 514
BccI CCATC 2 cut(s) 502, 530
BciT130I CCWGG 3 cut(s) 177, 399, 598
BciVI GTATCC 1 cut(s) 533
BfaI CTAG 1 cut(s) 116
BfuI GTATCC 1 cut(s) 533
BisI GCNGC 2 cut(s) 81, 503
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 82, 504
Bme1390I CCNGG 3 cut(s) 177, 399, 598
BmrFI CCNGG 3 cut(s) 177, 399, 598
BmsI GCATC 1 cut(s) 153
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 2 cut(s) 276, 498
Bsa29I ATCGAT 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 465
Bse3DI GCAATG 2 cut(s) 269, 429
BseBI CCWGG 3 cut(s) 177, 399, 598
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 541
BseMI GCAATG 2 cut(s) 269, 429
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 1 cut(s) 572
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 2 cut(s) 67, 514
BsgI GTGCAG 2 cut(s) 66, 338
BshTI ACCGGT 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 231
BsiSI CCGG 2 cut(s) 155, 471
BslFI GGGAC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 21
Bsp1286I GDGCHC 2 cut(s) 33, 566
Bsp143I GATC 4 cut(s) 194, 228, 232, 586
Bsp1720I GCTNAGC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 250
BspDI ATCGAT 1 cut(s) 231
BspPI GGATC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 269, 429
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 4 cut(s) 194, 228, 232, 586
Bst2UI CCWGG 3 cut(s) 177, 399, 598
Bst4CI ACNGT 3 cut(s) 104, 346, 461
Bst6I CTCTTC 2 cut(s) 180, 279
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 541
BstKTI GATC 4 cut(s) 197, 231, 235, 589
BstMBI GATC 4 cut(s) 194, 228, 232, 586
BstMWI GCNNNNNNNGC 3 cut(s) 151, 296, 499
BstNI CCWGG 3 cut(s) 177, 399, 598
BstSCI CCNGG 3 cut(s) 175, 397, 596
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 67, 514
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 533
BsuTUI ATCGAT 1 cut(s) 231
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 3 cut(s) 204, 359, 466
Cac8I GCNNGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 231
Csp6I GTAC 2 cut(s) 403, 468
CspAI ACCGGT 1 cut(s) 470
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 1 cut(s) 480
CviJI RGCY 9 cut(s) 127, 290, 299, 477, 493, 505, 512, 564, 604
CviKI_1 RGCY 9 cut(s) 127, 290, 299, 477, 493, 505, 512, 564, 604
CviQI GTAC 2 cut(s) 403, 468
DdeI CTNAG 1 cut(s) 258
DpnI GATC 4 cut(s) 196, 230, 234, 588
DpnII GATC 4 cut(s) 194, 228, 232, 586
Eam1104I CTCTTC 2 cut(s) 180, 279
EarI CTCTTC 2 cut(s) 180, 279
Eco24I GRGCYC 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 175, 397, 596
EcoT38I GRGCYC 1 cut(s) 566
FaeI CATG 1 cut(s) 483
FaqI GGGAC 1 cut(s) 21
FatI CATG 1 cut(s) 479
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 81, 503
FokI GGATG 1 cut(s) 548
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 2 cut(s) 81, 503
FspBI CTAG 1 cut(s) 116
GluI GCNGC 2 cut(s) 81, 503
HapII CCGG 2 cut(s) 155, 471
Hin1II CATG 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 1 cut(s) 57
HpaII CCGG 2 cut(s) 155, 471
Hpy166II GTNNAC 2 cut(s) 171, 307
Hpy188I TCNGA 2 cut(s) 68, 199
Hpy188III TCNNGA 2 cut(s) 293, 332
Hpy8I GTNNAC 2 cut(s) 171, 307
HpyAV CCTTC 2 cut(s) 31, 125
HpyCH4III ACNGT 3 cut(s) 104, 346, 461
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 3 cut(s) 83, 166, 319
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 296, 499
HpyF3I CTNAG 1 cut(s) 258
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 1 cut(s) 483
Kzo9I GATC 4 cut(s) 194, 228, 232, 586
Lsp1109I GCAGC 2 cut(s) 67, 514
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 179
MalI GATC 4 cut(s) 196, 230, 234, 588
MboI GATC 4 cut(s) 194, 228, 232, 586
MboII GAAGA 2 cut(s) 197, 296
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 2 cut(s) 33, 566
MluCI AATT 4 cut(s) 200, 374, 392, 526
MnlI CCTC 8 cut(s) 10, 20, 41, 74, 280, 289, 411, 550
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 2 cut(s) 90, 351
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 2 cut(s) 155, 471
MspR9I CCNGG 3 cut(s) 177, 399, 598
MvaI CCWGG 3 cut(s) 177, 399, 598
MwoI GCNNNNNNNGC 3 cut(s) 151, 296, 499
NdeII GATC 4 cut(s) 194, 228, 232, 586
NlaIII CATG 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 57
PfoI TCCNGGA 1 cut(s) 596
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 2 cut(s) 82, 504
PsiI TTATAA 1 cut(s) 390
Psp6I CCWGG 3 cut(s) 175, 397, 596
PspGI CCWGG 3 cut(s) 175, 397, 596
PsuI RGATCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 2 cut(s) 404, 469
RsaNI GTAC 2 cut(s) 403, 468
SaqAI TTAA 2 cut(s) 90, 351
SatI GCNGC 2 cut(s) 81, 503
Sau3AI GATC 4 cut(s) 194, 228, 232, 586
ScrFI CCNGG 3 cut(s) 177, 399, 598
SduI GDGCHC 2 cut(s) 33, 566
SetI ASST 5 cut(s) 79, 90, 428, 507, 606
SfaNI GCATC 1 cut(s) 153
SmlI CTYRAG 2 cut(s) 291, 513
SmoI CTYRAG 2 cut(s) 291, 513
Sse9I AATT 4 cut(s) 200, 374, 392, 526
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 175, 397, 596
TaaI ACNGT 3 cut(s) 104, 346, 461
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 231
TasI AATT 4 cut(s) 200, 374, 392, 526
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 2 cut(s) 90, 351
Tru9I TTAA 2 cut(s) 90, 351
TscAI CASTG 3 cut(s) 211, 366, 466
TseI GCWGC 2 cut(s) 80, 502
TspRI CASTG 3 cut(s) 211, 366, 466
XapI RAATTY 3 cut(s) 200, 374, 526
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.