Rorug05G0386300

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005233
Physical Location & Seq
Reverse (-)
52532768 .. 52534611
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0386300.1

Sequence Viewer

Length: 660 bp
ATGGAGATGGTCAAGAGTGCGATGGTGGTGGTGGTGCTAGTTTTGGTGGCGATGGTGCCGAGTCTGGAGGCGATTGAATCGCCGCAGTACACGGTGGTGCACTGGGAATCGGAGTTTGAGATAAGAGAATACAGAGCCGGCGCGTGGATGTCTGCTCCTGTTAAAGACATCTCCTTTGATAAGGCCACTAGAAATGGCTTCCACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAATTCCTCTCGAATTGCAATGACGGCTCCTGTGCTGACAAGCATAGTCCCTGGAGCCGGCCCGCTGCACTCATCCGCATACTTTGTGAGTTTCTATTTGCCAGTGAAGTTTCAAGCTTCCCCACCCACACCACTCCCGGAACTCAACTTGAAACCATATACATGGGACAGTCATTACGTTGCAGTCAGGAAATTCTCAGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTCGCCACCAGCCTTAGCAGGTCTCCATGGGCAAACTCTACATCAGAAGAAAGTAGCTATGCTTACTCGATTGCACAGTATAACTCCCCATTCCAGTGGGTTGGGCGTGTGAATGAAGTTTGGGTCGATGTCAATGCCTCTGGATTAGAGGGAGATAAAGGCGGTGAAATAGCTACCTACTGA

Protein Analysis

219

Amino Acids

24.33

Weight (kDa)

5.81

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 25 - 201 4.4e-55 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccB1I GGYRCC 2 cut(s) 55, 227
AccII CGCG 1 cut(s) 143
AciI CCGC 4 cut(s) 83, 299, 312, 639
AcsI RAATTY 2 cut(s) 238, 428
AfaI GTAC 2 cut(s) 89, 218
AfiI CCNNNNNNNGG 2 cut(s) 144, 293
AgsI TTSAA 4 cut(s) 77, 238, 350, 388
AjnI CCWGG 1 cut(s) 286
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 3 cut(s) 353, 534, 650
AluI AGCT 3 cut(s) 353, 534, 650
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 504
Alw44I GTGCAC 1 cut(s) 98
AoxI GGCC 2 cut(s) 183, 295
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 301
ApoI RAATTY 2 cut(s) 238, 428
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 102
BanI GGYRCC 2 cut(s) 55, 227
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 288
BccI CCATC 2 cut(s) 16, 46
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 2 cut(s) 38, 189
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 2 cut(s) 83, 302
BlsI GCNGC 2 cut(s) 84, 303
Bme1390I CCNGG 2 cut(s) 288, 374
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 57, 229, 264, 292
BmrFI CCNGG 2 cut(s) 288, 374
BmrI ACTGGG 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 112
BpmI CTGGAG 2 cut(s) 86, 309
Bpu10I CCTNAGC 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 374
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 3 cut(s) 223, 286, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 293
Bse118I RCCGGY 2 cut(s) 137, 293
Bse1I ACTGG 4 cut(s) 107, 214, 338, 571
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 3 cut(s) 223, 286, 503
BseGI GGATG 3 cut(s) 153, 219, 308
BseLI CCNNNNNNNGG 2 cut(s) 144, 293
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 4 cut(s) 107, 214, 338, 571
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 1 cut(s) 288
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 185, 297
BshNI GGYRCC 2 cut(s) 55, 227
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 3 cut(s) 138, 294, 374
BslFI GGGAC 2 cut(s) 269, 416
BslI CCNNNNNNNGG 2 cut(s) 144, 293
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 2 cut(s) 269, 416
BsnI GGCC 2 cut(s) 185, 297
Bso31I GGTCTC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 102
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 4 cut(s) 83, 299, 312, 639
BspANI GGCC 2 cut(s) 185, 297
BspCNI CTCAG 1 cut(s) 446
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 4 cut(s) 57, 229, 264, 292
BspMI ACCTGC 1 cut(s) 486
BspT107I GGYRCC 2 cut(s) 55, 227
BspTNI GGTCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 2 cut(s) 137, 293
BsrI ACTGG 4 cut(s) 107, 214, 338, 571
BssAI RCCGGY 2 cut(s) 137, 293
BssECI CCNNGG 3 cut(s) 223, 286, 503
BssT1I CCWWGG 2 cut(s) 223, 503
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 94, 407, 555
BstC8I GCNNGC 3 cut(s) 139, 295, 299
BstDEI CTNAG 2 cut(s) 433, 491
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 3 cut(s) 153, 219, 308
BstFNI CGCG 1 cut(s) 143
BstHHI GCGC 1 cut(s) 143
BstMAI GTCTC 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 286, 372
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 288
BstXI CCANNNNNNTGG 3 cut(s) 399, 573, 578
BsuRI GGCC 2 cut(s) 185, 297
BtgI CCRYGG 1 cut(s) 503
BtgZI GCGATG 2 cut(s) 35, 65
BtsCI GGATG 3 cut(s) 153, 219, 308
BtsIMutI CAGTG 3 cut(s) 100, 345, 578
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 3 cut(s) 139, 295, 299
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 2 cut(s) 137, 293
Cfr13I GGNCC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 88, 217
CviAII CATG 2 cut(s) 399, 504
CviQI GTAC 2 cut(s) 88, 217
DdeI CTNAG 2 cut(s) 433, 491
Eco130I CCWWGG 2 cut(s) 223, 503
Eco31I GGTCTC 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 223, 503
ErhI CCWWGG 2 cut(s) 223, 503
FaeI CATG 2 cut(s) 402, 507
FaiI YATR 8 cut(s) 281, 316, 394, 396, 400, 505, 537, 558
FaqI GGGAC 2 cut(s) 269, 416
FatI CATG 2 cut(s) 398, 503
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 83, 302
FokI GGATG 3 cut(s) 160, 206, 295
Fsp4HI GCNGC 2 cut(s) 83, 302
FspBI CTAG 2 cut(s) 38, 189
GlaI GCGC 1 cut(s) 142
GluI GCNGC 2 cut(s) 83, 302
GsuI CTGGAG 2 cut(s) 86, 309
HaeIII GGCC 2 cut(s) 185, 297
HapII CCGG 3 cut(s) 138, 294, 374
HhaI GCGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 402, 507
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 3 cut(s) 61, 77, 107
HpaII CCGG 3 cut(s) 138, 294, 374
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 2 cut(s) 90, 100
Hpy188I TCNGA 2 cut(s) 112, 523
Hpy188III TCNNGA 6 cut(s) 13, 65, 246, 424, 435, 618
Hpy8I GTNNAC 2 cut(s) 90, 100
HpyCH4III ACNGT 3 cut(s) 94, 407, 555
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 7 cut(s) 100, 254, 304, 419, 443, 463, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 433, 491
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 2 cut(s) 402, 507
HspAI GCGC 1 cut(s) 141
KroI GCCGGC 2 cut(s) 137, 293
KroNI GCCGGC 2 cut(s) 139, 295
LmnI GCTCC 3 cut(s) 160, 268, 290
Lsp1109I GCAGC 1 cut(s) 288
MaeI CTAG 2 cut(s) 38, 189
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 452
MboII GAAGA 1 cut(s) 536
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 4 cut(s) 232, 238, 249, 428
MlyI GAGTC 1 cut(s) 70
MnlI CCTC 4 cut(s) 61, 253, 619, 625
MroNI GCCGGC 2 cut(s) 137, 293
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 3 cut(s) 95, 397, 571
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 3 cut(s) 138, 294, 374
MspR9I CCNGG 2 cut(s) 288, 374
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 260
NaeI GCCGGC 2 cut(s) 139, 295
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 1 cut(s) 503
NgoMIV GCCGGC 2 cut(s) 137, 293
NlaIII CATG 2 cut(s) 402, 507
NlaIV GGNNCC 4 cut(s) 57, 229, 264, 292
NmeAIII GCCGAG 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 95
PdiI GCCGGC 2 cut(s) 139, 295
PfeI GAWTC 2 cut(s) 77, 107
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 2 cut(s) 84, 303
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 4 cut(s) 57, 229, 264, 292
PspPI GGNCC 1 cut(s) 296
RsaI GTAC 2 cut(s) 89, 218
RsaNI GTAC 2 cut(s) 88, 217
RseI CAYNNNNRTG 3 cut(s) 95, 397, 571
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 2 cut(s) 83, 302
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 288, 374
SduI GDGCHC 1 cut(s) 102
SetI ASST 7 cut(s) 229, 355, 417, 500, 536, 652, 656
SmiMI CAYNNNNRTG 3 cut(s) 95, 397, 571
Sse9I AATT 4 cut(s) 232, 238, 249, 428
SsiI CCGC 4 cut(s) 83, 299, 312, 639
SspMI CTAG 2 cut(s) 38, 189
StyD4I CCNGG 2 cut(s) 286, 372
StyI CCWWGG 2 cut(s) 223, 503
TaaI ACNGT 3 cut(s) 94, 407, 555
TaiI ACGT 1 cut(s) 417
TaqI TCGA 3 cut(s) 247, 545, 603
TasI AATT 4 cut(s) 232, 238, 249, 428
TatI WGTACW 2 cut(s) 87, 216
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 2 cut(s) 77, 107
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 3 cut(s) 107, 345, 578
TseI GCWGC 1 cut(s) 301
TspDTI ATGAA 1 cut(s) 606
TspRI CASTG 3 cut(s) 107, 345, 578
VneI GTGCAC 1 cut(s) 98
XapI RAATTY 2 cut(s) 238, 428
XspI CTAG 2 cut(s) 38, 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.