RchiOBHm_Chr5g0067931

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005233
Physical Location & Seq
Forward (+)
74022704 .. 74027182
4479 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34358

Sequence Viewer

Length: 1569 bp
ATGATGTACATGGCATCTGACCCATCTACGCAGGCCAAGGTTACTTCTGCATTCCAACAAGCCTCCAAGTATAAGATGAATGTTGCTAGAACTTGGGCTTTCAGTGATGGTGTTGGGAAAGCCCTTCAAACATCGCCTGGTTCGTATAACGAAGATATGTTCAAGGCTAAGGGTAGCGGAGCTGAGCTCTGGGGTATGGTTCTCTGCCCAGGTTTAGGCGAGTTTGTTCGACCTGCAGAGGAGGTAGCTGAAAGGTGCTCGGTGCCTGGTTCTGTGGGTGCGGCGACGAAGGAATCATTGATTTGTATGGTGAGTATGAATATGGCGGCAGGTTCGGTGGTGGCTATAGGTGATGAGGGTGGAAGGGTGCCCAGATCAGATTGCTGGCCCAAGTCAAGTTGGGGGCCCAAATCATGCTGGGTGTCCAAAACAGTTGAGATGTTTTCCTCGATTTGGACCTGTTTTCTGACCAAACTGATGGTCTCTGGCCCTTTAGGGTTTCAGATTTGGGATCCGGGAGGATTGACCCAACTACCTACGCCTATGTTTGACACAGGTGATACAAGTTTGCATACTATGGGGTTGATTTTACGCCAAGTAAATGAATTTTCTGGAGTACTACAATTGTGTGCATTGGCAAAGGAGGATTCAAGCTACTTTACTTTGTGTGATATGACCTTCAATAGATATGGATTAGACTTTGTGATATCAGAAGCTAAAAAGTACGGTGTATATCTGATACTGAGTTTAGTGAACAACTATAACGATTTTGGGGGAAGAAAGCAATATGTGCAGTGGGCAAGGGAGCGGGGCCAATCCCTCAACAGTGATGACGACTTCTACAACAACACTGTTGTCAAAGGCTACTACCAGAACCATGTCAAGACTATTCTCACGAGAATTAATACAATAACGAATGTGGCATACAAAGATGATCCAATCATTTTCGCATGGGAGCTAATCAATGAACTCCGTTGCGAATCTGATGTCTCGGGAGCTCTTCTTCAGCAATGGGTGAACGAAATGGCTGCACACTTAAAATCCATTGATGGCAACCATTTACTGGAGATAGGACTTGAAGGATTTTATGGGGCAACGACGCCGGAGAAGCAGCAATATAATCCCAATAACCAAGAGTTGGGCTCTGATTTCACAGCAACCAACTTGCTTCCACAAGTGGATTTTGCCACTATACATATCTACGCCGAGCAATGGTTATCCGGACAGAGCGAAGAGGCTCAAGCTGCTTTCGTGGATAAATGGGTGCAAGTACACATTGACGATTGTAATACAATAATGAAGAAACCACTTATTATGGCGGAGTTCGGCAAGTCTTGGAAATATCCAGGGTACACCATAGAAAACAGAAACGCCTACTTTGGAAAGTTATACGACGCCGAATACAGCAGCGCCAAAAGTGGGGGAGCGTGTGTAGGTGGACTTTTCTGGCAGCTGTTTGCGCAAGGTATGGAAAACTTCGGGGACGGATACCAAGTCGTTTTAGAGGAGAGCCCGTCAACTGCTGATGTCATTGCCCAGCAGTCTCAAAGGCTCCAGTCGCTAAATTAA

Protein Analysis

522

Amino Acids

57.92

Weight (kDa)

4.94

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 230 - 484 7.1e-34 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1461
Acc36I ACCTGC 2 cut(s) 241, 320
AccB1I GGYRCC 2 cut(s) 262, 367
AccB7I CCANNNNNTGG 2 cut(s) 1063, 1138
AccBSI CCGCTC 1 cut(s) 808
AccIII TCCGGA 1 cut(s) 1220
AciI CCGC 5 cut(s) 177, 281, 326, 808, 1319
AclWI GGATC 3 cut(s) 506, 519, 929
AcsI RAATTY 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 989
AcyI GRCGYC 2 cut(s) 1100, 1395
AfaI GTAC 5 cut(s) 8, 618, 725, 1272, 1352
AfiI CCNNNNNNNGG 6 cut(s) 215, 453, 1063, 1138, 1212, 1419
AgsI TTSAA 5 cut(s) 128, 163, 651, 682, 1079
AjnI CCWGG 4 cut(s) 136, 208, 265, 1345
AluBI AGCT 9 cut(s) 182, 187, 248, 654, 716, 958, 998, 1244, 1453
AluI AGCT 9 cut(s) 182, 187, 248, 654, 716, 958, 998, 1244, 1453
Alw21I GWGCWC 3 cut(s) 189, 260, 1000
Alw26I GTCTC 3 cut(s) 487, 994, 1548
AlwI GGATC 3 cut(s) 506, 519, 929
Ama87I CYCGRG 1 cut(s) 991
Aor13HI TCCGGA 1 cut(s) 1220
AoxI GGCC 5 cut(s) 33, 386, 404, 487, 811
ApaI GGGCCC 1 cut(s) 408
ApeKI GCWGC 5 cut(s) 1028, 1111, 1244, 1407, 1450
ApoI RAATTY 1 cut(s) 605
ArsI GACNNNNNNTTYG 4 cut(s) 465, 497, 972, 1004
AseI ATTAAT 1 cut(s) 903
AspLEI GCGC 2 cut(s) 1412, 1462
AspS9I GGNCC 6 cut(s) 387, 404, 405, 456, 488, 811
AsuC2I CCSGG 1 cut(s) 516
AsuHPI GGTGA 4 cut(s) 322, 362, 569, 1027
AvaI CYCGRG 1 cut(s) 991
AvaII GGWCC 1 cut(s) 456
BaeGI GKGCMC 2 cut(s) 372, 408
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 2 cut(s) 262, 367
BanII GRGCYC 5 cut(s) 189, 408, 1000, 1145, 1514
BauI CACGAG 1 cut(s) 895
Bbv12I GWGCWC 3 cut(s) 189, 260, 1000
BbvI GCAGC 5 cut(s) 1015, 1123, 1231, 1419, 1462
BccI CCATC 4 cut(s) 31, 101, 472, 1043
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 4 cut(s) 138, 210, 267, 1347
BciVI GTATCC 1 cut(s) 1481
BcnI CCSGG 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 487, 994, 1548
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 2 cut(s) 234, 345
BfoI RGCGCY 1 cut(s) 1413
BfuAI ACCTGC 2 cut(s) 241, 320
BfuI GTATCC 1 cut(s) 1481
BisI GCNGC 7 cut(s) 282, 327, 1029, 1112, 1245, 1408, 1451
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 7 cut(s) 283, 328, 1030, 1113, 1246, 1409, 1452
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 5 cut(s) 138, 210, 267, 516, 1347
Bme18I GGWCC 1 cut(s) 456
BmeT110I CYCGRG 1 cut(s) 991
BmgT120I GGNCC 6 cut(s) 387, 404, 405, 456, 488, 811
BmiI GGNNCC 7 cut(s) 264, 369, 405, 406, 513, 812, 1553
BmrFI CCNGG 5 cut(s) 138, 210, 267, 516, 1347
BmsI GCATC 1 cut(s) 23
BplI GAGNNNNNCTC 4 cut(s) 171, 203, 1127, 1159
BpmI CTGGAG 3 cut(s) 633, 1085, 1538
Bpu10I CCTNAGC 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 1224
BpuMI CCSGG 1 cut(s) 516
BsaHI GRCGYC 2 cut(s) 1100, 1395
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 36, 208, 1346
BsaWI WCCGGW 1 cut(s) 1220
BsaXI ACNNNNNCTCC 4 cut(s) 1416, 1446, 1499, 1529
Bsc4I CCNNNNNNNGG 6 cut(s) 215, 453, 1063, 1138, 1212, 1419
Bse1I ACTGG 2 cut(s) 1068, 1555
Bse3DI GCAATG 3 cut(s) 1016, 1217, 1530
BseAI TCCGGA 1 cut(s) 1220
BseBI CCWGG 4 cut(s) 138, 210, 267, 1347
BseDI CCNNGG 3 cut(s) 36, 208, 1346
BseLI CCNNNNNNNGG 6 cut(s) 215, 453, 1063, 1138, 1212, 1419
BseMI GCAATG 3 cut(s) 1016, 1217, 1530
BseMII CTCAG 2 cut(s) 174, 734
BseNI ACTGG 2 cut(s) 1068, 1555
BseRI GAGGAG 2 cut(s) 254, 1520
BseSI GKGCMC 2 cut(s) 372, 408
BseXI GCAGC 5 cut(s) 1015, 1123, 1231, 1419, 1462
BseYI CCCAGC 2 cut(s) 417, 1536
BsgI GTGCAG 2 cut(s) 812, 1014
BshFI GGCC 5 cut(s) 35, 388, 406, 489, 813
BshNI GGYRCC 2 cut(s) 262, 367
BsiHKAI GWGCWC 3 cut(s) 189, 260, 1000
BsiHKCI CYCGRG 1 cut(s) 991
BsiSI CCGG 3 cut(s) 515, 1103, 1221
BslFI GGGAC 1 cut(s) 1496
BslI CCNNNNNNNGG 6 cut(s) 215, 453, 1063, 1138, 1212, 1419
BsmAI GTCTC 3 cut(s) 487, 994, 1548
BsmFI GGGAC 1 cut(s) 1496
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 5 cut(s) 35, 388, 406, 489, 813
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 991
Bsp120I GGGCCC 1 cut(s) 404
Bsp1286I GDGCHC 7 cut(s) 189, 260, 372, 408, 1000, 1145, 1514
Bsp13I TCCGGA 1 cut(s) 1220
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 3 cut(s) 374, 511, 934
Bsp1720I GCTNAGC 1 cut(s) 183
BspACI CCGC 5 cut(s) 177, 281, 326, 808, 1319
BspANI GGCC 5 cut(s) 35, 388, 406, 489, 813
BspCNI CTCAG 2 cut(s) 175, 735
BspEI TCCGGA 1 cut(s) 1220
BspLI GGNNCC 7 cut(s) 264, 369, 405, 406, 513, 812, 1553
BspMAI CTGCAG 1 cut(s) 238
BspMI ACCTGC 2 cut(s) 241, 320
BspPI GGATC 3 cut(s) 506, 519, 929
BspQI GCTCTTC 1 cut(s) 1005
BspT107I GGYRCC 2 cut(s) 262, 367
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 808
BsrDI GCAATG 3 cut(s) 1016, 1217, 1530
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 2 cut(s) 1068, 1555
BssECI CCNNGG 3 cut(s) 36, 208, 1346
BssMI GATC 3 cut(s) 374, 511, 934
BssNI GRCGYC 2 cut(s) 1100, 1395
BssSI CACGAG 1 cut(s) 895
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 895
Bst2UI CCWGG 4 cut(s) 138, 210, 267, 1347
Bst4CI ACNGT 4 cut(s) 433, 728, 827, 853
Bst6I CTCTTC 2 cut(s) 1005, 1227
BstACI GRCGYC 2 cut(s) 1100, 1395
BstAPI GCANNNNNTGC 1 cut(s) 790
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 33, 386
BstDEI CTNAG 3 cut(s) 168, 183, 743
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 2 cut(s) 1412, 1462
BstKTI GATC 3 cut(s) 377, 514, 937
BstMAI GTCTC 3 cut(s) 487, 994, 1548
BstMBI GATC 3 cut(s) 374, 511, 934
BstMWI GCNNNNNNNGC 5 cut(s) 790, 1108, 1244, 1459, 1558
BstNI CCWGG 4 cut(s) 138, 210, 267, 1347
BstSCI CCNGG 5 cut(s) 136, 208, 265, 514, 1345
BstSFI CTRYAG 2 cut(s) 234, 345
BstSLI GKGCMC 2 cut(s) 372, 408
BstV1I GCAGC 5 cut(s) 1015, 1123, 1231, 1419, 1462
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 511
BsuI GTATCC 1 cut(s) 1481
BsuRI GGCC 5 cut(s) 35, 388, 406, 489, 813
BtgZI GCGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 800
BtsIMutI CAGTG 4 cut(s) 109, 800, 832, 849
BveI ACCTGC 2 cut(s) 241, 320
Cac8I GCNNGC 2 cut(s) 33, 386
CfoI GCGC 2 cut(s) 1412, 1462
Cfr13I GGNCC 6 cut(s) 387, 404, 405, 456, 488, 811
CseI GACGC 2 cut(s) 1108, 1403
Csp6I GTAC 5 cut(s) 7, 617, 724, 1271, 1351
CviAII CATG 4 cut(s) 10, 414, 878, 951
CviQI GTAC 5 cut(s) 7, 617, 724, 1271, 1351
DdeI CTNAG 3 cut(s) 168, 183, 743
DpnI GATC 3 cut(s) 376, 513, 936
DpnII GATC 3 cut(s) 374, 511, 934
Eam1104I CTCTTC 2 cut(s) 1005, 1227
EarI CTCTTC 2 cut(s) 1005, 1227
EciI GGCGGA 1 cut(s) 1334
Ecl136II GAGCTC 2 cut(s) 187, 998
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 5 cut(s) 189, 408, 1000, 1145, 1514
Eco31I GGTCTC 1 cut(s) 487
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 456
Eco53kI GAGCTC 2 cut(s) 187, 998
Eco57I CTGAAG 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 991
EcoICRI GAGCTC 2 cut(s) 187, 998
EcoO109I RGGNCCY 1 cut(s) 404
EcoRII CCWGG 4 cut(s) 136, 208, 265, 1345
EcoRV GATATC 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 5 cut(s) 189, 408, 1000, 1145, 1514
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 4 cut(s) 13, 417, 881, 954
FaqI GGGAC 1 cut(s) 1496
FatI CATG 4 cut(s) 9, 413, 877, 950
FauI CCCGC 1 cut(s) 801
Fnu4HI GCNGC 7 cut(s) 282, 327, 1029, 1112, 1245, 1408, 1451
FriOI GRGCYC 5 cut(s) 189, 408, 1000, 1145, 1514
Fsp4HI GCNGC 7 cut(s) 282, 327, 1029, 1112, 1245, 1408, 1451
FspBI CTAG 1 cut(s) 87
FspI TGCGCA 1 cut(s) 1461
GlaI GCGC 2 cut(s) 1411, 1461
GluI GCNGC 7 cut(s) 282, 327, 1029, 1112, 1245, 1408, 1451
GsaI CCCAGC 2 cut(s) 421, 1540
GsuI CTGGAG 3 cut(s) 633, 1085, 1538
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 5 cut(s) 35, 388, 406, 489, 813
HapII CCGG 3 cut(s) 515, 1103, 1221
HgaI GACGC 2 cut(s) 1108, 1403
HhaI GCGC 2 cut(s) 1412, 1462
Hin1I GRCGYC 2 cut(s) 1100, 1395
Hin1II CATG 4 cut(s) 13, 417, 881, 954
Hin6I GCGC 2 cut(s) 1410, 1460
HinP1I GCGC 2 cut(s) 1410, 1460
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HinfI GANTC 3 cut(s) 293, 647, 980
HpaII CCGG 3 cut(s) 515, 1103, 1221
HphI GGTGA 4 cut(s) 322, 362, 569, 1027
Hpy166II GTNNAC 6 cut(s) 754, 1018, 1273, 1353, 1439, 1518
Hpy188I TCNGA 8 cut(s) 19, 379, 468, 504, 712, 738, 985, 1147
Hpy188III TCNNGA 5 cut(s) 612, 883, 895, 993, 1221
Hpy8I GTNNAC 6 cut(s) 754, 1018, 1273, 1353, 1439, 1518
Hpy99I CGWCG 3 cut(s) 289, 1102, 1397
HpyAV CCTTC 5 cut(s) 134, 283, 357, 688, 1073
HpyCH4III ACNGT 4 cut(s) 433, 728, 827, 853
HpyCH4V TGCA 7 cut(s) 50, 236, 571, 632, 793, 1031, 1267
HpyF10VI GCNNNNNNNGC 5 cut(s) 790, 1108, 1244, 1459, 1558
HpyF3I CTNAG 3 cut(s) 168, 183, 743
Hsp92I GRCGYC 2 cut(s) 1100, 1395
Hsp92II CATG 4 cut(s) 13, 417, 881, 954
HspAI GCGC 2 cut(s) 1410, 1460
Kpn2I TCCGGA 1 cut(s) 1220
Kzo9I GATC 3 cut(s) 374, 511, 934
LguI GCTCTTC 1 cut(s) 1005
LmnI GCTCC 6 cut(s) 179, 805, 955, 995, 1424, 1557
Lsp1109I GCAGC 5 cut(s) 1015, 1123, 1231, 1419, 1462
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 40
MalI GATC 3 cut(s) 376, 513, 936
MbiI CCGCTC 1 cut(s) 808
MboI GATC 3 cut(s) 374, 511, 934
MboII GAAGA 6 cut(s) 164, 789, 992, 995, 1244, 1312
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 7 cut(s) 189, 260, 372, 408, 1000, 1145, 1514
MluCI AATT 4 cut(s) 605, 623, 900, 1564
MmeI TCCRAC 1 cut(s) 79
MroI TCCGGA 1 cut(s) 1220
MseI TTAA 3 cut(s) 903, 1037, 1567
MspA1I CMGCKG 1 cut(s) 1453
MspI CCGG 3 cut(s) 515, 1103, 1221
MspR9I CCNGG 5 cut(s) 138, 210, 267, 516, 1347
MunI CAATTG 1 cut(s) 623
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 4 cut(s) 138, 210, 267, 1347
MwoI GCNNNNNNNGC 5 cut(s) 790, 1108, 1244, 1459, 1558
NciI CCSGG 1 cut(s) 516
NdeII GATC 3 cut(s) 374, 511, 934
NlaIII CATG 4 cut(s) 13, 417, 881, 954
NlaIV GGNNCC 7 cut(s) 264, 369, 405, 406, 513, 812, 1553
NmeAIII GCCGAG 1 cut(s) 1231
NsbI TGCGCA 1 cut(s) 1461
PciSI GCTCTTC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 1 cut(s) 50
PfeI GAWTC 3 cut(s) 293, 647, 980
PflMI CCANNNNNTGG 2 cut(s) 1063, 1138
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 7 cut(s) 283, 328, 1030, 1113, 1246, 1409, 1452
PshBI ATTAAT 1 cut(s) 903
Psp124BI GAGCTC 2 cut(s) 189, 1000
Psp6I CCWGG 4 cut(s) 136, 208, 265, 1345
PspFI CCCAGC 2 cut(s) 417, 1536
PspGI CCWGG 4 cut(s) 136, 208, 265, 1345
PspN4I GGNNCC 7 cut(s) 264, 369, 405, 406, 513, 812, 1553
PspOMI GGGCCC 1 cut(s) 404
PspPI GGNCC 6 cut(s) 387, 404, 405, 456, 488, 811
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 511
PvuII CAGCTG 1 cut(s) 1453
RsaI GTAC 5 cut(s) 8, 618, 725, 1272, 1352
RsaNI GTAC 5 cut(s) 7, 617, 724, 1271, 1351
SacI GAGCTC 2 cut(s) 189, 1000
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 3 cut(s) 903, 1037, 1567
SatI GCNGC 7 cut(s) 282, 327, 1029, 1112, 1245, 1408, 1451
Sau3AI GATC 3 cut(s) 374, 511, 934
Sau96I GGNCC 6 cut(s) 387, 404, 405, 456, 488, 811
ScaI AGTACT 1 cut(s) 618
ScrFI CCNGG 5 cut(s) 138, 210, 267, 516, 1347
SduI GDGCHC 7 cut(s) 189, 260, 372, 408, 1000, 1145, 1514
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 2 cut(s) 234, 345
SinI GGWCC 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 1239
SmoI CTYRAG 1 cut(s) 1239
Sse9I AATT 4 cut(s) 605, 623, 900, 1564
SsiI CCGC 5 cut(s) 177, 281, 326, 808, 1319
SspMI CTAG 1 cut(s) 87
SstI GAGCTC 2 cut(s) 189, 1000
StyD4I CCNGG 5 cut(s) 136, 208, 265, 514, 1345
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 4 cut(s) 433, 728, 827, 853
TaqI TCGA 2 cut(s) 229, 449
TasI AATT 4 cut(s) 605, 623, 900, 1564
TatI WGTACW 3 cut(s) 6, 616, 1270
TauI GCSGC 2 cut(s) 284, 329
TfiI GAWTC 3 cut(s) 293, 647, 980
Tru1I TTAA 3 cut(s) 903, 1037, 1567
Tru9I TTAA 3 cut(s) 903, 1037, 1567
TscAI CASTG 4 cut(s) 109, 800, 832, 856
TseI GCWGC 5 cut(s) 1028, 1111, 1244, 1407, 1450
TspDTI ATGAA 5 cut(s) 92, 332, 618, 981, 1313
TspGWI ACGGA 2 cut(s) 962, 1500
TspRI CASTG 4 cut(s) 109, 800, 832, 856
Van91I CCANNNNNTGG 2 cut(s) 1063, 1138
VpaK11BI GGWCC 1 cut(s) 456
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 1 cut(s) 605
XspI CTAG 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.