Rh5BG462900

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005233
Physical Location & Seq
Forward (+)
73983679 .. 73985706
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG462900.1

Sequence Viewer

Length: 1068 bp
ATGAGCTTCAGATCTCCACACTCTCCATCTCCACCAAGCAAACCGAGCTCCAGACCTCACAGTCTCATCTCCACAAGCTCATCGCCCTCAAGACCCTCCCCGTCTTCCACGGCCGCGCCGGCAACGAGAACTCCCTCGACTGGCTCACCGCCACCTAGCGCCACTTTGCCTACCTCGACATCCCCGCCGCTAACCGAGTCAAGATCGCCGCCACTCGTTTGCACCGCGACGCGTGCCCCTGGATGTGTTGTGAACAACTACAACGATTTTGGGGGAAGAAAGCAATATGTGCAGTGGGCAAGGGAGCGGGGCCAATCCGTCAACAGTGATGACGACTTCTACAACAACACTGTTGTCAAAGGCTACTACAAGAACCATGTCAAGACTATTCTCACGAGAATTAATACAATAACGAATGTGGCATACAAAGATGATCCAATCATTTTCGCCTGGGAGCTAATCAATGAACCACGTTGCGAATCTGATGTCTCGGGAGCTCTTCTTCAGCAATGGATGAACGAAATGGCTGCACACTTAAAATCCATTGATGGCAACCATTTACTGGAGATAGGACTTGAAGGATTTTATGGGGCAACGACGCCGGAGAAGCAGCAATATAATCCCAATAACCAAGAGTTGGGCTCTGATTTCATAGCAACCAACTTGCTTCCACAAGTGGATTTTGCCACTATACATATCTACGCCGAGCAATGGTTATCCGGACAGAGCGAAGAGGCTCAAGCTGCTTTCGTGGATAAATGGGTGCAAGTACACATTGACGATTGTAATACAATAGTGAAGAAACCACTTATTATGGCGGAGTTCGGCAAGTCTTGGAAATATCCAGGGTTTTCCATAGAAAACAGAAACGCCTACTTTCGAAAGTTATACGACGCCGTATACAGCAGCGCCAAAAGTGGGGGAGCGTGTGTAGGTGGACTTTTCTGGCAGCTGTTTGCGCAAGGTATGGAAAACTTCGGGGACGGATACCAAGTCGTTTTAGAGGAGAGCCCGTCAACAGCTGATGTCATCGCCCAGCAGTCTCAAAGGCTCCAGTCGCTAAATTAA

Protein Analysis

355

Amino Acids

39.14

Weight (kDa)

5.83

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 85 - 317 9e-32 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 60
Acc16I TGCGCA 1 cut(s) 960
AccB7I CCANNNNNTGG 2 cut(s) 562, 637
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 900
AccII CGCG 3 cut(s) 116, 227, 232
AccIII TCCGGA 1 cut(s) 719
AciI CCGC 8 cut(s) 114, 149, 185, 188, 209, 225, 307, 818
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 488
AcyI GRCGYC 2 cut(s) 599, 894
AfaI GTAC 1 cut(s) 771
AfiI CCNNNNNNNGG 5 cut(s) 140, 562, 637, 711, 918
AflIII ACRYGT 1 cut(s) 230
AgsI TTSAA 1 cut(s) 578
AjnI CCWGG 3 cut(s) 238, 449, 844
AluBI AGCT 8 cut(s) 6, 48, 78, 457, 497, 743, 952, 1022
AluI AGCT 8 cut(s) 6, 48, 78, 457, 497, 743, 952, 1022
Alw21I GWGCWC 2 cut(s) 50, 499
Alw26I GTCTC 3 cut(s) 68, 493, 1047
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 719
AoxI GGCC 2 cut(s) 111, 310
ApeKI GCWGC 5 cut(s) 527, 610, 743, 906, 949
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
AseI ATTAAT 1 cut(s) 402
AspLEI GCGC 4 cut(s) 118, 161, 911, 961
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 138
AsuII TTCGAA 1 cut(s) 880
AvaI CYCGRG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 238
BanII GRGCYC 4 cut(s) 50, 499, 644, 1013
BauI CACGAG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 50, 499
BbvI GCAGC 5 cut(s) 514, 622, 730, 918, 961
BccI CCATC 2 cut(s) 34, 542
BceAI ACGGC 2 cut(s) 126, 881
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 3 cut(s) 240, 451, 846
BciVI GTATCC 1 cut(s) 980
BcoDI GTCTC 3 cut(s) 68, 493, 1047
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 2 cut(s) 162, 912
BfuI GTATCC 1 cut(s) 980
BglI GCCNNNNNGGC 1 cut(s) 119
BglII AGATCT 1 cut(s) 11
BisI GCNGC 8 cut(s) 114, 188, 209, 528, 611, 744, 907, 950
BlsI GCNGC 8 cut(s) 115, 189, 210, 529, 612, 745, 908, 951
Bme1390I CCNGG 3 cut(s) 240, 451, 846
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 2 cut(s) 311, 1052
BmrFI CCNGG 3 cut(s) 240, 451, 846
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 626, 658
BpmI CTGGAG 3 cut(s) 34, 584, 1037
Bpu14I TTCGAA 1 cut(s) 880
BpuEI CTTGAG 2 cut(s) 73, 723
BsaHI GRCGYC 2 cut(s) 599, 894
BsaJI CCNNGG 4 cut(s) 108, 238, 450, 845
BsaWI WCCGGW 1 cut(s) 719
BsaXI ACNNNNNCTCC 6 cut(s) 115, 145, 915, 945, 998, 1028
Bsc4I CCNNNNNNNGG 5 cut(s) 140, 562, 637, 711, 918
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 3 cut(s) 145, 567, 1054
Bse3DI GCAATG 2 cut(s) 515, 716
BseAI TCCGGA 1 cut(s) 719
BseBI CCWGG 3 cut(s) 240, 451, 846
BseDI CCNNGG 4 cut(s) 108, 238, 450, 845
BseGI GGATG 3 cut(s) 179, 248, 519
BseLI CCNNNNNNNGG 5 cut(s) 140, 562, 637, 711, 918
BseMI GCAATG 2 cut(s) 515, 716
BseNI ACTGG 3 cut(s) 145, 567, 1054
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 238
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 5 cut(s) 514, 622, 730, 918, 961
BseYI CCCAGC 1 cut(s) 1035
BsgI GTGCAG 2 cut(s) 311, 513
Bsh1236I CGCG 3 cut(s) 116, 227, 232
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 113, 312
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 2 cut(s) 50, 499
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 3 cut(s) 119, 602, 720
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 5 cut(s) 140, 562, 637, 711, 918
BsmAI GTCTC 3 cut(s) 68, 493, 1047
BsmFI GGGAC 1 cut(s) 995
BsnI GGCC 2 cut(s) 113, 312
BsoBI CYCGRG 1 cut(s) 490
Bsp119I TTCGAA 1 cut(s) 880
Bsp1286I GDGCHC 5 cut(s) 50, 238, 499, 644, 1013
Bsp13I TCCGGA 1 cut(s) 719
Bsp143I GATC 3 cut(s) 11, 203, 433
BspACI CCGC 8 cut(s) 114, 149, 185, 188, 209, 225, 307, 818
BspANI GGCC 2 cut(s) 113, 312
BspEI TCCGGA 1 cut(s) 719
BspFNI CGCG 3 cut(s) 116, 227, 232
BspLI GGNNCC 2 cut(s) 311, 1052
BspPI GGATC 1 cut(s) 428
BspQI GCTCTTC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 880
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 515, 716
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 3 cut(s) 145, 567, 1054
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 4 cut(s) 108, 238, 450, 845
BssMI GATC 3 cut(s) 11, 203, 433
BssNAI GTATAC 1 cut(s) 901
BssNI GRCGYC 2 cut(s) 599, 894
BssSI CACGAG 1 cut(s) 394
Bst1107I GTATAC 1 cut(s) 901
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 3 cut(s) 240, 451, 846
Bst4CI ACNGT 3 cut(s) 62, 326, 352
Bst6I CTCTTC 2 cut(s) 504, 726
BstACI GRCGYC 2 cut(s) 599, 894
BstAPI GCANNNNNTGC 1 cut(s) 289
BstBI TTCGAA 1 cut(s) 880
BstC8I GCNNGC 2 cut(s) 120, 234
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 3 cut(s) 179, 248, 519
BstFNI CGCG 3 cut(s) 116, 227, 232
BstH2I RGCGCY 2 cut(s) 162, 912
BstHHI GCGC 4 cut(s) 118, 161, 911, 961
BstKTI GATC 3 cut(s) 14, 206, 436
BstMAI GTCTC 3 cut(s) 68, 493, 1047
BstMBI GATC 3 cut(s) 11, 203, 433
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 8 cut(s) 45, 119, 233, 289, 607, 743, 958, 1057
BstNI CCWGG 3 cut(s) 240, 451, 846
BstSCI CCNGG 3 cut(s) 238, 449, 844
BstSLI GKGCMC 1 cut(s) 238
BstUI CGCG 3 cut(s) 116, 227, 232
BstV1I GCAGC 5 cut(s) 514, 622, 730, 918, 961
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 901
BstZI CGGCCG 1 cut(s) 111
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 2 cut(s) 113, 312
BtgI CCRYGG 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 66, 1015
BtsCI GGATG 3 cut(s) 179, 248, 519
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 3 cut(s) 299, 331, 348
Cac8I GCNNGC 2 cut(s) 120, 234
CfoI GCGC 4 cut(s) 118, 161, 911, 961
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 3 cut(s) 238, 607, 902
Csp6I GTAC 1 cut(s) 770
CviAII CATG 1 cut(s) 377
CviQI GTAC 1 cut(s) 770
DpnI GATC 3 cut(s) 13, 205, 435
DpnII GATC 3 cut(s) 11, 203, 433
DrdI GACNNNNNNGTC 1 cut(s) 60
DseDI GACNNNNNNGTC 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 111
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 2 cut(s) 504, 726
EarI CTCTTC 2 cut(s) 504, 726
EciI GGCGGA 1 cut(s) 833
Ecl136II GAGCTC 2 cut(s) 48, 497
EclXI CGGCCG 1 cut(s) 111
Eco24I GRGCYC 4 cut(s) 50, 499, 644, 1013
Eco52I CGGCCG 1 cut(s) 111
Eco53kI GAGCTC 2 cut(s) 48, 497
Eco57I CTGAAG 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 490
EcoICRI GAGCTC 2 cut(s) 48, 497
EcoRII CCWGG 3 cut(s) 238, 449, 844
EcoT38I GRGCYC 4 cut(s) 50, 499, 644, 1013
FaeI CATG 1 cut(s) 380
FaqI GGGAC 1 cut(s) 995
FatI CATG 1 cut(s) 376
FauI CCCGC 2 cut(s) 192, 300
FblI GTMKAC 1 cut(s) 900
Fnu4HI GCNGC 8 cut(s) 114, 188, 209, 528, 611, 744, 907, 950
FokI GGATG 3 cut(s) 166, 255, 526
FriOI GRGCYC 4 cut(s) 50, 499, 644, 1013
Fsp4HI GCNGC 8 cut(s) 114, 188, 209, 528, 611, 744, 907, 950
FspBI CTAG 1 cut(s) 156
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 4 cut(s) 117, 160, 910, 960
GluI GCNGC 8 cut(s) 114, 188, 209, 528, 611, 744, 907, 950
GsaI CCCAGC 1 cut(s) 1039
GsuI CTGGAG 3 cut(s) 34, 584, 1037
HaeII RGCGCY 2 cut(s) 162, 912
HaeIII GGCC 2 cut(s) 113, 312
HapII CCGG 3 cut(s) 119, 602, 720
HgaI GACGC 3 cut(s) 238, 607, 902
HhaI GCGC 4 cut(s) 118, 161, 911, 961
Hin1I GRCGYC 2 cut(s) 599, 894
Hin1II CATG 1 cut(s) 380
Hin6I GCGC 4 cut(s) 116, 159, 909, 959
HinP1I GCGC 4 cut(s) 116, 159, 909, 959
HincII GTYRAC 2 cut(s) 322, 1017
HindII GTYRAC 2 cut(s) 322, 1017
HinfI GANTC 2 cut(s) 197, 479
HpaII CCGG 3 cut(s) 119, 602, 720
HphI GGTGA 1 cut(s) 138
Hpy166II GTNNAC 6 cut(s) 253, 322, 772, 901, 938, 1017
Hpy188I TCNGA 3 cut(s) 11, 484, 646
Hpy188III TCNNGA 7 cut(s) 51, 90, 201, 382, 394, 492, 720
Hpy8I GTNNAC 6 cut(s) 253, 322, 772, 901, 938, 1017
Hpy99I CGWCG 3 cut(s) 232, 601, 896
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 3 cut(s) 62, 326, 352
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 4 cut(s) 222, 292, 530, 766
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 119, 233, 289, 607, 743, 958, 1057
HpySE526I ACGT 1 cut(s) 472
Hsp92I GRCGYC 2 cut(s) 599, 894
Hsp92II CATG 1 cut(s) 380
HspAI GCGC 4 cut(s) 116, 159, 909, 959
Kpn2I TCCGGA 1 cut(s) 719
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 3 cut(s) 11, 203, 433
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 6 cut(s) 53, 304, 454, 494, 923, 1056
Lsp1109I GCAGC 5 cut(s) 514, 622, 730, 918, 961
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 472
MalI GATC 3 cut(s) 13, 205, 435
MbiI CCGCTC 1 cut(s) 307
MboI GATC 3 cut(s) 11, 203, 433
MboII GAAGA 6 cut(s) 96, 288, 491, 494, 743, 811
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 5 cut(s) 50, 238, 499, 644, 1013
MluCI AATT 2 cut(s) 399, 1063
MluI ACGCGT 1 cut(s) 230
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 66, 97, 106, 145, 184, 727, 997
MroI TCCGGA 1 cut(s) 719
MroNI GCCGGC 1 cut(s) 118
MseI TTAA 3 cut(s) 402, 536, 1066
MspA1I CMGCKG 2 cut(s) 952, 1022
MspI CCGG 3 cut(s) 119, 602, 720
MspR9I CCNGG 3 cut(s) 240, 451, 846
MvaI CCWGG 3 cut(s) 240, 451, 846
MvnI CGCG 3 cut(s) 116, 227, 232
MwoI GCNNNNNNNGC 8 cut(s) 45, 119, 233, 289, 607, 743, 958, 1057
NaeI GCCGGC 1 cut(s) 120
NdeII GATC 3 cut(s) 11, 203, 433
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 2 cut(s) 311, 1052
NmeAIII GCCGAG 1 cut(s) 730
NsbI TGCGCA 1 cut(s) 960
NspV TTCGAA 1 cut(s) 880
PciSI GCTCTTC 1 cut(s) 504
PdiI GCCGGC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 479
PflMI CCANNNNNTGG 2 cut(s) 562, 637
PkrI GCNGC 8 cut(s) 115, 189, 210, 529, 612, 745, 908, 951
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PshBI ATTAAT 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 50, 499
Psp6I CCWGG 3 cut(s) 238, 449, 844
PspFI CCCAGC 1 cut(s) 1035
PspGI CCWGG 3 cut(s) 238, 449, 844
PspN4I GGNNCC 2 cut(s) 311, 1052
PspPI GGNCC 1 cut(s) 310
PsuI RGATCY 1 cut(s) 11
PvuII CAGCTG 2 cut(s) 952, 1022
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
SacI GAGCTC 2 cut(s) 50, 499
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 3 cut(s) 402, 536, 1066
SatI GCNGC 8 cut(s) 114, 188, 209, 528, 611, 744, 907, 950
Sau3AI GATC 3 cut(s) 11, 203, 433
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 3 cut(s) 240, 451, 846
SduI GDGCHC 5 cut(s) 50, 238, 499, 644, 1013
SfuI TTCGAA 1 cut(s) 880
SmlI CTYRAG 2 cut(s) 88, 738
SmoI CTYRAG 2 cut(s) 88, 738
Sse9I AATT 2 cut(s) 399, 1063
SsiI CCGC 8 cut(s) 114, 149, 185, 188, 209, 225, 307, 818
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 2 cut(s) 50, 499
StyD4I CCNGG 3 cut(s) 238, 449, 844
TaaI ACNGT 3 cut(s) 62, 326, 352
TaiI ACGT 1 cut(s) 475
TaqI TCGA 3 cut(s) 137, 176, 880
TasI AATT 2 cut(s) 399, 1063
TatI WGTACW 1 cut(s) 769
TauI GCSGC 3 cut(s) 116, 190, 211
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 3 cut(s) 402, 536, 1066
Tru9I TTAA 3 cut(s) 402, 536, 1066
TscAI CASTG 3 cut(s) 299, 331, 355
TseI GCWGC 5 cut(s) 527, 610, 743, 906, 949
TspDTI ATGAA 3 cut(s) 480, 530, 640
TspGWI ACGGA 2 cut(s) 307, 999
TspRI CASTG 3 cut(s) 299, 331, 355
Van91I CCANNNNNTGG 2 cut(s) 562, 637
VspI ATTAAT 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 900
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.