Rh5AG445500

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005233
Physical Location & Seq
Forward (+)
76598941 .. 76600317
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG445500.1

Sequence Viewer

Length: 744 bp
ATGCAATATGTGCAGTGGGCTACGGAGTGGGGCCACTCCTTCAACAGTGATGACGACTTCTACAACAACACTGTTGTCAAAGGCTACTACAAGAACCATGTCAAGACTATTCTCACGAGAATTAATACAATAACAAATATGACATACAAAGATGATTCAACCATTTTCGCATGGGAGCTAATCAATGAACCCTGTTGTGAATCTGATGTCTCGGGAGCTCTTCTTCAGCAATGGGTGAACGAAATGGCTGCACACTTAAAATCCATTGATGGCAACCATTTACTGGAGATAGGACTTGAAGGATTTTATGGGGCAACGACGCAGGAGAAGAAGCAATATAATCCCGGTAACCAAGAGTTGGGCTCTGATTTCATAGCAACCAACTTGCTTCCACAAGTGGATTTTGCCACTATACATATCTACGCCGAGCAATGGTACGTGCAAGTACACATTGACGATTGTAATACAATAGTGAAGAAACCACTTATTATGGCGGAGTTCGGCAAGTCTTGGAAATATCCAGGGTACTCCAAAGAAAACAGAAACGCCTACTTTGGAAAGTTATACGACGCCGTATACAGCAGCGCCAAAAGTGGGGGAGCGTGTGTAGGTGGACTTTTCTGGCAGCTGTTTGCGCAAGGTATGGAAAACTTCGGGGACGGATACCAAGTCGTTTTAGAGGAGAGCCCGTCAACTGCTGATGTCATCGCCCAGCAGTCTCAAAGGCTCCGGTCGCTAAATTAA

Protein Analysis

247

Amino Acids

28.03

Weight (kDa)

4.92

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH5_mannosidase PF26410 4 - 209 5.9e-28 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 636
AccB7I CCANNNNNTGG 2 cut(s) 283, 358
AccI GTMKAC 1 cut(s) 576
AciI CCGC 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 3 cut(s) 437, 447, 527
AfiI CCNNNNNNNGG 4 cut(s) 283, 358, 432, 594
AgsI TTSAA 3 cut(s) 43, 159, 299
AjnI CCWGG 1 cut(s) 520
AluBI AGCT 3 cut(s) 178, 218, 628
AluI AGCT 3 cut(s) 178, 218, 628
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 214, 723
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 3 cut(s) 248, 582, 625
AseI ATTAAT 1 cut(s) 123
AspLEI GCGC 2 cut(s) 587, 637
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 211
BanII GRGCYC 3 cut(s) 220, 365, 689
BauI CACGAG 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 3 cut(s) 235, 594, 637
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 557
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 1 cut(s) 656
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 214, 723
BfoI RGCGCY 1 cut(s) 588
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 3 cut(s) 249, 583, 626
BlsI GCNGC 3 cut(s) 250, 584, 627
Bme1390I CCNGG 2 cut(s) 345, 522
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 32, 728
BmrFI CCNGG 2 cut(s) 345, 522
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 521
BsaWI WCCGGW 1 cut(s) 729
BsaXI ACNNNNNCTCC 4 cut(s) 591, 621, 674, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 358, 432, 594
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 2 cut(s) 236, 437
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 521
BseLI CCNNNNNNNGG 4 cut(s) 283, 358, 432, 594
BseMI GCAATG 2 cut(s) 236, 437
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 695
BseXI GCAGC 3 cut(s) 235, 594, 637
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 2 cut(s) 32, 234
Bsh1285I CGRYCG 1 cut(s) 734
BshFI GGCC 1 cut(s) 33
BsiEI CGRYCG 1 cut(s) 734
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 2 cut(s) 345, 730
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 4 cut(s) 283, 358, 432, 594
BsmAI GTCTC 2 cut(s) 214, 723
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 3 cut(s) 220, 365, 689
BspACI CCGC 1 cut(s) 494
BspANI GGCC 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 32, 728
BspQI GCTCTTC 1 cut(s) 225
BsrDI GCAATG 2 cut(s) 236, 437
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 521
BssNAI GTATAC 1 cut(s) 577
BssNI GRCGYC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 115
Bst1107I GTATAC 1 cut(s) 577
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 2 cut(s) 47, 73
Bst6I CTCTTC 1 cut(s) 225
BstACI GRCGYC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 439
BstEII GGTNACC 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 2 cut(s) 587, 637
BstMAI GTCTC 2 cut(s) 214, 723
BstMCI CGRYCG 1 cut(s) 734
BstMWI GCNNNNNNNGC 3 cut(s) 10, 634, 733
BstNI CCWGG 1 cut(s) 522
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 343, 520
BstV1I GCAGC 3 cut(s) 235, 594, 637
BstZ17I GTATAC 1 cut(s) 577
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 1 cut(s) 33
BtgZI GCGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 20
BtsIMutI CAGTG 3 cut(s) 20, 52, 69
CfoI GCGC 2 cut(s) 587, 637
Cfr13I GGNCC 1 cut(s) 31
CseI GACGC 2 cut(s) 328, 578
Csp6I GTAC 3 cut(s) 436, 446, 526
CviAII CATG 2 cut(s) 98, 171
CviQI GTAC 3 cut(s) 436, 446, 526
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 509
Ecl136II GAGCTC 1 cut(s) 218
Eco24I GRGCYC 3 cut(s) 220, 365, 689
Eco53kI GAGCTC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 211
Eco91I GGTNACC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 520
EcoT38I GRGCYC 3 cut(s) 220, 365, 689
FaeI CATG 2 cut(s) 101, 174
FaqI GGGAC 1 cut(s) 671
FatI CATG 2 cut(s) 97, 170
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 3 cut(s) 249, 583, 626
FriOI GRGCYC 3 cut(s) 220, 365, 689
Fsp4HI GCNGC 3 cut(s) 249, 583, 626
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 2 cut(s) 586, 636
GluI GCNGC 3 cut(s) 249, 583, 626
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 1 cut(s) 33
HapII CCGG 2 cut(s) 345, 730
HgaI GACGC 2 cut(s) 328, 578
HhaI GCGC 2 cut(s) 587, 637
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 2 cut(s) 101, 174
Hin6I GCGC 2 cut(s) 585, 635
HinP1I GCGC 2 cut(s) 585, 635
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HinfI GANTC 2 cut(s) 155, 200
HpaII CCGG 2 cut(s) 345, 730
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 5 cut(s) 238, 448, 577, 614, 693
Hpy188I TCNGA 2 cut(s) 205, 367
Hpy188III TCNNGA 3 cut(s) 103, 115, 213
Hpy8I GTNNAC 5 cut(s) 238, 448, 577, 614, 693
Hpy99I CGWCG 2 cut(s) 322, 572
HpyAV CCTTC 2 cut(s) 49, 293
HpyCH4III ACNGT 2 cut(s) 47, 73
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 4 cut(s) 4, 13, 251, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 634, 733
HpySE526I ACGT 1 cut(s) 438
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 2 cut(s) 101, 174
HspAI GCGC 2 cut(s) 585, 635
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 4 cut(s) 175, 215, 599, 732
LpnPI CCDG 8 cut(s) 205, 269, 308, 358, 507, 534, 607, 725
Lsp1109I GCAGC 3 cut(s) 235, 594, 637
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 347
MboII GAAGA 4 cut(s) 212, 215, 340, 487
MhlI GDGCHC 3 cut(s) 220, 365, 689
MluCI AATT 2 cut(s) 120, 739
MnlI CCTC 1 cut(s) 673
MseI TTAA 3 cut(s) 123, 257, 742
MspA1I CMGCKG 1 cut(s) 628
MspI CCGG 2 cut(s) 345, 730
MspR9I CCNGG 2 cut(s) 345, 522
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 10, 634, 733
NciI CCSGG 1 cut(s) 345
NlaIII CATG 2 cut(s) 101, 174
NlaIV GGNNCC 2 cut(s) 32, 728
NmeAIII GCCGAG 1 cut(s) 451
NsbI TGCGCA 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 155, 200
PflMI CCANNNNNTGG 2 cut(s) 283, 358
PkrI GCNGC 3 cut(s) 250, 584, 627
Ppu21I YACGTR 1 cut(s) 439
PshBI ATTAAT 1 cut(s) 123
Psp124BI GAGCTC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 520
PspEI GGTNACC 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 32, 728
PspPI GGNCC 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 3 cut(s) 437, 447, 527
RsaNI GTAC 3 cut(s) 436, 446, 526
SacI GAGCTC 1 cut(s) 220
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 3 cut(s) 123, 257, 742
SatI GCNGC 3 cut(s) 249, 583, 626
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 345, 522
SduI GDGCHC 3 cut(s) 220, 365, 689
SetI ASST 6 cut(s) 180, 220, 441, 613, 630, 643
Sse9I AATT 2 cut(s) 120, 739
SsiI CCGC 1 cut(s) 494
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 2 cut(s) 343, 520
TaaI ACNGT 2 cut(s) 47, 73
TaiI ACGT 1 cut(s) 441
TasI AATT 2 cut(s) 120, 739
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 2 cut(s) 155, 200
Tru1I TTAA 3 cut(s) 123, 257, 742
Tru9I TTAA 3 cut(s) 123, 257, 742
TscAI CASTG 3 cut(s) 20, 52, 76
TseI GCWGC 3 cut(s) 248, 582, 625
TspDTI ATGAA 2 cut(s) 201, 361
TspGWI ACGGA 2 cut(s) 38, 675
TspRI CASTG 3 cut(s) 20, 52, 76
Van91I CCANNNNNTGG 2 cut(s) 283, 358
VspI ATTAAT 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.