Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005233

Physical Location & Seq

Genomic Coordinates Reverse (-)

70107625 .. 70109895

Gene Structure 2271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG485600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGCCTTTGTTACGTACCTGTATTGTTTTATCTTCTTTAGTGTTCCTCAATATCCAGCAGGGAAATTATTGCAGAGGTGAAACCCAGGCTACCTCTAGTAGTTTTGCTCAAACAAAAGGAAACCATTTTGTTATGAATGAGAGATCATTCTACTTGAATGGGTTCAATGCATATTGGATGATGTACATGGCATCTGACCCCTCTACAAGGGCCAAGGTCACTTCTGCATTCAAACAAGCCTCCAAGTATGGTATGAACATTGCCAGAACTTGGGCTTTCAGCGATGGCGGTAGCTACAGACCCCTTCAGTCTTCTCCTGGTTCCTATAACGAAGACATGTTCAAGGGATTAGACTTTGTGATCTCAGAAGCTAGAAATTATGGTGTGCATGTGATACTGAGCTTGGTGAACAACTTCAACGATTATGGGGGGAAAAAGCAATATGTGCAGTGGGCAAAGGAACGTGGCCAAGCCATAAACAACGAAGACGACTTCTATAACAACACTGTTGTCAAAGGCTACTATAAAGATCACGTTAAGACTGTTCTCACGAGAATCAACTCCATTACTGGGGTGGCTTACAGGGATGATTCAACCATTTTTTCTTGGGAATTAATGAACGAGCCTCGTTGTCAACTTGATCCCTCTGGAACCCTTCTCCAGCAATGGGTGAAAGAAATGGCTGCTCACGTCAAGTCCATTGATAGTAACCATTTACTGGAGATAGGGCTTGAAGGATTCTATGGTGGAACAACCCCGGAAAAGAACCAGTTCAATCCTAGTAACCTAGAATATGGGTCTGATTTCATAGCCAATAACTTGATTCCCGAGATTGACTTTGCCTCGATTCATATATACGCTGAGCAATGGTTACGAGGGGCAAGTGAAGAGGCACAAGCTGATTTTGTGGACAAATGGGTCGGAGTACACATTGAAGACTGTAACTCAGTGGTAAGAAAACCACTAATTATGGGGGAGTTTGGCAAATCTTACAAACTGCCAGGGTACACCATAGAAAAGAGGAACGCCTACTTCGAAAAACTTTACGACGTCGTCTACCGCAGCGCAAGCAGTAACGGAGCATGCACAGGTGCGCTTTTCTGGCAGCTGCTTGCTCAAGGAATGGATAACTTCAAAGACGGATATGATGTCGTTCTGGAGGAGAGCCCTTCCACGGCTAGTGTGATTGCTCAACAATCTCACAAGCTAATCGAGTTCACCAGGCAACATAGTAATGTTACAAAAAGTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010412 GO:0015923 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0046355 GO:0071554 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

417

Amino Acids

47.16

Weight (kDa)

5.89

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH5_mannosidase	PF26410	34 - 369	1.6e-50	GH family 5 beta-mannosidase-like
Cellulase	PF00150	53 - 344	1.6e-12	Cellulase (glycosyl hydrolase family 5)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005233)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02310 AT1G02310
fragaria_vesca	FvH4_6g08680 FvH4_6g36270 FvH4_6g36270
malus_domestica	MD09G1170400.v1.1 MD17G1156500.v1.1
prunus_persica	Prupe.3G028800_v2.0.a1
pyrus_communis	pycom09g08810
rosa_chinensis	RchiOBHm_Chr2g0148271 RchiOBHm_Chr5g0067931
rosa_laevigata	RLG00000020331 RLG00000035944
rosa_multiflora	Rmu_sc0003937.1_g000001
rosa_roxburghii	Rroxscaffold_1G00012680 Rroxscaffold_1G00013380 Rroxscaffold_2G00098950
rosa_rugosa	Rorug02G0405200 Rorug05G0386300
rosa_samantha	Rh2AG463800 Rh2BG476900 Rh2CG450600 Rh2DG485600 Rh5AG445500 Rh5BG462900 Rh5CG484800 Rh5DG476900
rosa_wichuraiana	Rw2G037810 Rw5G041600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	917
AatII	GACGTC	1 cut(s)	1053
AccB7I	CCANNNNNTGG	2 cut(s)	270, 718
AccI	GTMKAC	1 cut(s)	1056
AciI	CCGC	2 cut(s)	288, 1060
AclWI	GGATC	1 cut(s)	635
AcoI	YGGCCR	1 cut(s)	466
AcuI	CTGAAG	1 cut(s)	290
AcyI	GRCGYC	1 cut(s)	1050
AfaI	GTAC	5 cut(s)	16, 185, 927, 1007, 1249
AfiI	CCNNNNNNNGG	6 cut(s)	207, 270, 570, 667, 718, 1174
AflIII	ACRYGT	1 cut(s)	336
AjiI	CACGTC	1 cut(s)	691
AjnI	CCWGG	4 cut(s)	84, 316, 1000, 1220
AluBI	AGCT	6 cut(s)	294, 371, 402, 899, 1108, 1207
AluI	AGCT	6 cut(s)	294, 371, 402, 899, 1108, 1207
AlwI	GGATC	1 cut(s)	635
Ama87I	CYCGRG	1 cut(s)	827
AoxI	GGCC	2 cut(s)	210, 466
ApeKI	GCWGC	4 cut(s)	683, 1062, 1105, 1108
AseI	ATTAAT	1 cut(s)	614
Asp700I	GAANNNNTTC	3 cut(s)	161, 413, 770
AspLEI	GCGC	2 cut(s)	1067, 1096
AspS9I	GGNCC	1 cut(s)	210
AsuC2I	CCSGG	1 cut(s)	758
AsuHPI	GGTGA	4 cut(s)	89, 418, 682, 1210
AsuII	TTCGAA	1 cut(s)	1035
AvaI	CYCGRG	1 cut(s)	827
BalI	TGGCCA	1 cut(s)	468
BanII	GRGCYC	1 cut(s)	1169
BauI	CACGAG	1 cut(s)	550
BbsI	GAAGAC	4 cut(s)	303, 339, 492, 942
BbvI	GCAGC	4 cut(s)	670, 1074, 1095, 1117
BccI	CCATC	1 cut(s)	278
BceAI	ACGGC	1 cut(s)	1191
BciT130I	CCWGG	4 cut(s)	86, 318, 1002, 1222
BcnI	CCSGG	1 cut(s)	758
BfaI	CTAG	5 cut(s)	96, 372, 780, 788, 1179
BfmI	CTRYAG	1 cut(s)	295
BisI	GCNGC	4 cut(s)	684, 1063, 1106, 1109
BlpI	GCTNAGC	1 cut(s)	861
BlsI	GCNGC	4 cut(s)	685, 1064, 1107, 1110
BmcAI	AGTACT	1 cut(s)	1249
Bme1390I	CCNGG	5 cut(s)	86, 318, 758, 1002, 1222
BmeT110I	CYCGRG	1 cut(s)	827
BmgBI	CACGTC	1 cut(s)	691
BmgT120I	GGNCC	1 cut(s)	210
BmiI	GGNNCC	2 cut(s)	322, 652
BmrFI	CCNGG	5 cut(s)	86, 318, 758, 1002, 1222
BmrI	ACTGGG	1 cut(s)	579
BmsI	GCATC	1 cut(s)	200
BmuI	ACTGGG	1 cut(s)	579
BpiI	GAAGAC	4 cut(s)	303, 339, 492, 942
BpmI	CTGGAG	3 cut(s)	644, 740, 1178
Bpu1102I	GCTNAGC	1 cut(s)	861
Bpu14I	TTCGAA	1 cut(s)	1035
BpuEI	CTTGAG	1 cut(s)	1101
BpuMI	CCSGG	1 cut(s)	758
BsaAI	YACGTR	1 cut(s)	14
BsaHI	GRCGYC	1 cut(s)	1050
BsaJI	CCNNGG	5 cut(s)	84, 213, 756, 1001, 1173
Bsc4I	CCNNNNNNNGG	6 cut(s)	207, 270, 570, 667, 718, 1174
Bse1I	ACTGG	3 cut(s)	574, 723, 769
Bse3DI	GCAATG	3 cut(s)	258, 671, 872
BseBI	CCWGG	4 cut(s)	86, 318, 1002, 1222
BseDI	CCNNGG	5 cut(s)	84, 213, 756, 1001, 1173
BseGI	GGATG	2 cut(s)	183, 592
BseLI	CCNNNNNNNGG	6 cut(s)	207, 270, 570, 667, 718, 1174
BseMI	GCAATG	3 cut(s)	258, 671, 872
BseMII	CTCAG	4 cut(s)	378, 389, 852, 960
BseNI	ACTGG	3 cut(s)	574, 723, 769
BseRI	GAGGAG	1 cut(s)	1175
BseXI	GCAGC	4 cut(s)	670, 1074, 1095, 1117
BsgI	GTGCAG	1 cut(s)	467
BshFI	GGCC	2 cut(s)	212, 468
BsiHKCI	CYCGRG	1 cut(s)	827
BsiSI	CCGG	1 cut(s)	758
BslI	CCNNNNNNNGG	6 cut(s)	207, 270, 570, 667, 718, 1174
BsmI	GAATGC	1 cut(s)	227
BsnI	GGCC	2 cut(s)	212, 468
BsoBI	CYCGRG	1 cut(s)	827
Bsp119I	TTCGAA	1 cut(s)	1035
Bsp1286I	GDGCHC	1 cut(s)	1169
Bsp1407I	TGTACA	1 cut(s)	183
Bsp143I	GATC	4 cut(s)	143, 360, 529, 640
Bsp1720I	GCTNAGC	1 cut(s)	861
BspACI	CCGC	2 cut(s)	288, 1060
BspANI	GGCC	2 cut(s)	212, 468
BspCNI	CTCAG	4 cut(s)	377, 390, 853, 959
BspLI	GGNNCC	2 cut(s)	322, 652
BspPI	GGATC	1 cut(s)	635
BspT104I	TTCGAA	1 cut(s)	1035
BsrDI	GCAATG	3 cut(s)	258, 671, 872
BsrGI	TGTACA	1 cut(s)	183
BsrI	ACTGG	3 cut(s)	574, 723, 769
BssECI	CCNNGG	5 cut(s)	84, 213, 756, 1001, 1173
BssMI	GATC	4 cut(s)	143, 360, 529, 640
BssNI	GRCGYC	1 cut(s)	1050
BssSI	CACGAG	1 cut(s)	550
BssT1I	CCWWGG	1 cut(s)	213
Bst2BI	CACGAG	1 cut(s)	550
Bst2UI	CCWGG	4 cut(s)	86, 318, 1002, 1222
Bst4CI	ACNGT	3 cut(s)	508, 544, 941
Bst6I	CTCTTC	1 cut(s)	882
BstACI	GRCGYC	1 cut(s)	1050
BstAPI	GCANNNNNTGC	1 cut(s)	445
BstAUI	TGTACA	1 cut(s)	183
BstBAI	YACGTR	1 cut(s)	14
BstBI	TTCGAA	1 cut(s)	1035
BstC8I	GCNNGC	3 cut(s)	1069, 1084, 1113
BstDEI	CTNAG	4 cut(s)	364, 398, 861, 946
BstDSI	CCRYGG	1 cut(s)	1173
BstENI	CCTNNNNNAGG	1 cut(s)	205
BstF5I	GGATG	2 cut(s)	183, 592
BstHHI	GCGC	2 cut(s)	1067, 1096
BstKTI	GATC	4 cut(s)	146, 363, 532, 643
BstMBI	GATC	4 cut(s)	143, 360, 529, 640
BstMWI	GCNNNNNNNGC	3 cut(s)	445, 1068, 1102
BstNI	CCWGG	4 cut(s)	86, 318, 1002, 1222
BstNSI	RCATGY	3 cut(s)	340, 392, 1086
BstSCI	CCNGG	5 cut(s)	84, 316, 756, 1000, 1220
BstSFI	CTRYAG	1 cut(s)	295
BstSNI	TACGTA	1 cut(s)	14
BstV1I	GCAGC	4 cut(s)	670, 1074, 1095, 1117
BstV2I	GAAGAC	4 cut(s)	303, 339, 492, 942
BsuRI	GGCC	2 cut(s)	212, 468
BtgI	CCRYGG	1 cut(s)	1173
BtgZI	GCGATG	1 cut(s)	297
BtrI	CACGTC	1 cut(s)	691
BtsCI	GGATG	2 cut(s)	183, 592
BtsI	GCAGTG	1 cut(s)	455
BtsIMutI	CAGTG	3 cut(s)	455, 504, 954
Cac8I	GCNNGC	3 cut(s)	1069, 1084, 1113
CfoI	GCGC	2 cut(s)	1067, 1096
Cfr13I	GGNCC	1 cut(s)	210
Csp6I	GTAC	5 cut(s)	15, 184, 926, 1006, 1248
CviAII	CATG	4 cut(s)	187, 337, 389, 1083
CviQI	GTAC	5 cut(s)	15, 184, 926, 1006, 1248
DdeI	CTNAG	4 cut(s)	364, 398, 861, 946
DpnI	GATC	4 cut(s)	145, 362, 531, 642
DpnII	GATC	4 cut(s)	143, 360, 529, 640
DrdI	GACNNNNNNGTC	1 cut(s)	917
DseDI	GACNNNNNNGTC	1 cut(s)	917
EaeI	YGGCCR	1 cut(s)	466
Eam1104I	CTCTTC	1 cut(s)	882
EarI	CTCTTC	1 cut(s)	882
Eco105I	TACGTA	1 cut(s)	14
Eco130I	CCWWGG	1 cut(s)	213
Eco24I	GRGCYC	1 cut(s)	1169
Eco57I	CTGAAG	1 cut(s)	290
Eco88I	CYCGRG	1 cut(s)	827
EcoNI	CCTNNNNNAGG	1 cut(s)	205
EcoRII	CCWGG	4 cut(s)	84, 316, 1000, 1220
EcoT14I	CCWWGG	1 cut(s)	213
EcoT22I	ATGCAT	1 cut(s)	172
EcoT38I	GRGCYC	1 cut(s)	1169
ErhI	CCWWGG	1 cut(s)	213
FaeI	CATG	4 cut(s)	190, 340, 392, 1086
FatI	CATG	4 cut(s)	186, 336, 388, 1082
FblI	GTMKAC	1 cut(s)	1056
Fnu4HI	GCNGC	4 cut(s)	684, 1063, 1106, 1109
FokI	GGATG	2 cut(s)	190, 599
FriOI	GRGCYC	1 cut(s)	1169
Fsp4HI	GCNGC	4 cut(s)	684, 1063, 1106, 1109
FspBI	CTAG	5 cut(s)	96, 372, 780, 788, 1179
GlaI	GCGC	2 cut(s)	1066, 1095
GluI	GCNGC	4 cut(s)	684, 1063, 1106, 1109
GsuI	CTGGAG	3 cut(s)	644, 740, 1178
HaeIII	GGCC	2 cut(s)	212, 468
HapII	CCGG	1 cut(s)	758
HhaI	GCGC	2 cut(s)	1067, 1096
Hin1I	GRCGYC	1 cut(s)	1050
Hin1II	CATG	4 cut(s)	190, 340, 392, 1086
Hin6I	GCGC	2 cut(s)	1065, 1094
HinP1I	GCGC	2 cut(s)	1065, 1094
HincII	GTYRAC	1 cut(s)	635
HindII	GTYRAC	1 cut(s)	635
HinfI	GANTC	5 cut(s)	555, 590, 738, 823, 847
HpaII	CCGG	1 cut(s)	758
HphI	GGTGA	4 cut(s)	89, 418, 682, 1210
Hpy166II	GTNNAC	7 cut(s)	409, 635, 910, 928, 1008, 1057, 1218
Hpy188I	TCNGA	4 cut(s)	196, 367, 802, 923
Hpy188III	TCNNGA	4 cut(s)	550, 648, 827, 1157
Hpy8I	GTNNAC	7 cut(s)	409, 635, 910, 928, 1008, 1057, 1218
Hpy99I	CGWCG	2 cut(s)	1052, 1055
HpyAV	CCTTC	4 cut(s)	314, 665, 728, 1179
HpyCH4III	ACNGT	3 cut(s)	508, 544, 941
HpyCH4IV	ACGT	5 cut(s)	13, 463, 534, 690, 1050
HpyCH4V	TGCA	6 cut(s)	72, 170, 227, 388, 448, 1086
HpyF10VI	GCNNNNNNNGC	3 cut(s)	445, 1068, 1102
HpyF3I	CTNAG	4 cut(s)	364, 398, 861, 946
HpySE526I	ACGT	5 cut(s)	13, 463, 534, 690, 1050
Hsp92I	GRCGYC	1 cut(s)	1050
Hsp92II	CATG	4 cut(s)	190, 340, 392, 1086
HspAI	GCGC	2 cut(s)	1065, 1094
Kzo9I	GATC	4 cut(s)	143, 360, 529, 640
LmnI	GCTCC	1 cut(s)	1079
Lsp1109I	GCAGC	4 cut(s)	670, 1074, 1095, 1117
LweI	GCATC	1 cut(s)	200
MaeI	CTAG	5 cut(s)	96, 372, 780, 788, 1179
MaeII	ACGT	5 cut(s)	13, 463, 534, 690, 1050
MaeIII	GTNAC	8 cut(s)	9, 217, 707, 782, 870, 941, 1073, 1237
MalI	GATC	4 cut(s)	145, 362, 531, 642
MboI	GATC	4 cut(s)	143, 360, 529, 640
MboII	GAAGA	6 cut(s)	24, 303, 344, 497, 899, 947
MhlI	GDGCHC	1 cut(s)	1169
MlsI	TGGCCA	1 cut(s)	468
MluCI	AATT	4 cut(s)	64, 376, 611, 966
MluNI	TGGCCA	1 cut(s)	468
MmeI	TCCRAC	1 cut(s)	901
Mox20I	TGGCCA	1 cut(s)	468
Mph1103I	ATGCAT	1 cut(s)	172
MroXI	GAANNNNTTC	3 cut(s)	161, 413, 770
MscI	TGGCCA	1 cut(s)	468
MseI	TTAA	2 cut(s)	537, 614
MslI	CAYNNNNRTG	1 cut(s)	1233
Msp20I	TGGCCA	1 cut(s)	468
MspA1I	CMGCKG	1 cut(s)	1108
MspI	CCGG	1 cut(s)	758
MspR9I	CCNGG	5 cut(s)	86, 318, 758, 1002, 1222
Mva1269I	GAATGC	1 cut(s)	227
MvaI	CCWGG	4 cut(s)	86, 318, 1002, 1222
MwoI	GCNNNNNNNGC	3 cut(s)	445, 1068, 1102
NciI	CCSGG	1 cut(s)	758
NdeII	GATC	4 cut(s)	143, 360, 529, 640
NlaIII	CATG	4 cut(s)	190, 340, 392, 1086
NlaIV	GGNNCC	2 cut(s)	322, 652
NmuCI	GTSAC	1 cut(s)	217
NsiI	ATGCAT	1 cut(s)	172
NspI	RCATGY	3 cut(s)	340, 392, 1086
NspV	TTCGAA	1 cut(s)	1035
PaeI	GCATGC	1 cut(s)	1086
PciI	ACATGT	1 cut(s)	336
PcsI	WCGNNNNNNNCGW	1 cut(s)	1032
PctI	GAATGC	1 cut(s)	227
PdmI	GAANNNNTTC	3 cut(s)	161, 413, 770
PfeI	GAWTC	5 cut(s)	555, 590, 738, 823, 847
PflFI	GACNNNGTC	1 cut(s)	1052
PflMI	CCANNNNNTGG	2 cut(s)	270, 718
PkrI	GCNGC	4 cut(s)	685, 1064, 1107, 1110
Ppu21I	YACGTR	1 cut(s)	14
PscI	ACATGT	1 cut(s)	336
PshBI	ATTAAT	1 cut(s)	614
Psp6I	CCWGG	4 cut(s)	84, 316, 1000, 1220
PspGI	CCWGG	4 cut(s)	84, 316, 1000, 1220
PspN4I	GGNNCC	2 cut(s)	322, 652
PspPI	GGNCC	1 cut(s)	210
PsyI	GACNNNGTC	1 cut(s)	1052
PvuII	CAGCTG	1 cut(s)	1108
RsaI	GTAC	5 cut(s)	16, 185, 927, 1007, 1249
RsaNI	GTAC	5 cut(s)	15, 184, 926, 1006, 1248
RseI	CAYNNNNRTG	1 cut(s)	1233
SaqAI	TTAA	2 cut(s)	537, 614
SatI	GCNGC	4 cut(s)	684, 1063, 1106, 1109
Sau3AI	GATC	4 cut(s)	143, 360, 529, 640
Sau96I	GGNCC	1 cut(s)	210
ScaI	AGTACT	1 cut(s)	1249
ScrFI	CCNGG	5 cut(s)	86, 318, 758, 1002, 1222
SduI	GDGCHC	1 cut(s)	1169
SfaNI	GCATC	1 cut(s)	200
SfcI	CTRYAG	1 cut(s)	295
SfuI	TTCGAA	1 cut(s)	1035
SmiMI	CAYNNNNRTG	1 cut(s)	1233
SmlI	CTYRAG	1 cut(s)	1116
SmoI	CTYRAG	1 cut(s)	1116
SnaBI	TACGTA	1 cut(s)	14
SphI	GCATGC	1 cut(s)	1086
Sse9I	AATT	4 cut(s)	64, 376, 611, 966
SsiI	CCGC	2 cut(s)	288, 1060
SspMI	CTAG	5 cut(s)	96, 372, 780, 788, 1179
StyD4I	CCNGG	5 cut(s)	84, 316, 756, 1000, 1220
StyI	CCWWGG	1 cut(s)	213
TaaI	ACNGT	3 cut(s)	508, 544, 941
TaiI	ACGT	5 cut(s)	16, 466, 537, 693, 1053
TaqI	TCGA	3 cut(s)	845, 1035, 1212
TasI	AATT	4 cut(s)	64, 376, 611, 966
TatI	WGTACW	3 cut(s)	183, 925, 1247
TfiI	GAWTC	5 cut(s)	555, 590, 738, 823, 847
Tru1I	TTAA	2 cut(s)	537, 614
Tru9I	TTAA	2 cut(s)	537, 614
TscAI	CASTG	3 cut(s)	455, 511, 954
TseFI	GTSAC	1 cut(s)	217
TseI	GCWGC	4 cut(s)	683, 1062, 1105, 1108
Tsp45I	GTSAC	1 cut(s)	217
TspDTI	ATGAA	5 cut(s)	149, 269, 632, 796, 839
TspGWI	ACGGA	2 cut(s)	1092, 1155
TspRI	CASTG	3 cut(s)	455, 511, 954
Tth111I	GACNNNGTC	1 cut(s)	1052
Van91I	CCANNNNNTGG	2 cut(s)	270, 718
VspI	ATTAAT	1 cut(s)	614
XagI	CCTNNNNNAGG	1 cut(s)	205
XceI	RCATGY	3 cut(s)	340, 392, 1086
XcmI	CCANNNNNNNNNTGG	1 cut(s)	571
XmiI	GTMKAC	1 cut(s)	1056
XmnI	GAANNNNTTC	3 cut(s)	161, 413, 770
XspI	CTAG	5 cut(s)	96, 372, 780, 788, 1179
ZraI	GACGTC	1 cut(s)	1051
ZrmI	AGTACT	1 cut(s)	1249
Zsp2I	ATGCAT	1 cut(s)	172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.