Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006094

Physical Location & Seq

Genomic Coordinates Forward (+)

31904791 .. 31907822

Gene Structure 3032 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1249700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGCGCGTACGGGCGTTGTGGTGTTGGGCGCTTCTGATATCATTAGCAGCACTAGATTCGCCGGCAGCAGCAGCGGCGTCGTCGTGGGCGGTGGACGGGACGGTGTTGGAGCTCGACGATTCCAACTTCGATTCCGCCATCTCCGCCTTGGACTTGGTCTTGGTTGATTTCTACGCCCCCTGGTGCGGCCACTGCAAGCGTCTCTCTCCTCAGTTGGATGCAGCTGCCCCCTTGCTTGCTGGGTTGAAACACCCAGTAGCGATTGCAAAACTTAATGCTGACAAATTCACCTCTGTAGCTCGAAAATACGAAATCGAGTATGATCCTGTTTCTTCAAATCCCTTTTATGTCCATTGCCATGCATATCCGACCCTCAAACTGTTTATGCACGGTGTTCCCATGGAATACAATGGACCCAGGAAAGCGGAATCGCTCGTTCGCTATCTGAAGAAGTTTGCAGCTCCTGATGTTTCCATACTTGAATCAGACTCTGCTATAAGTGAATTTGTCCAGGCTGCTGGCACTTACTTTCCTATATATATTGGTTTTGGGTTGAACGAGTCTTTGGTATCGAAGGTAGCAATAAAGTACAAAAAGAAGGCATGGTTTTCGGTTGCAAAGGATTTCTCCGAAGATGTAATGGTGTTGTATGATTTTGACAAGGTTCCTGCTTTGGCATCCCTTCATCCAACGTATGATGAGCGCAACATCTTCTATGGTCCCTTTGAAGAGGAGTTTTTGGAAGATTTTATAAGACAAAGTTTGTTCCCTTTGGCCATGCCCATAAACTATGAGACATTAAAGTCGTTGAGTGATGATGAAAGAAAAATTGTTTTGACAATCGTGGAAGATGAGGATGAAGAGAAGTCTAAGAAACTTATCAATATTTTAAAATCAGCGGCATCTGCAAATCGGGACTTTGTATTTGGTTATGTTGGGATCAAACAGTGGGAAGACTTTGCTGATACCTTTGGAGCCAACAAGAAGACGAAACTACCAAAAATGGTTGTCTGGGATCGAATGGAGGAGTACATTACAGTAAATGGGTCAGAAAGCATTGATGAGGAAGATCAGGCATCTCAAGTCTCACAGTTTATACAGGGATACAAGGAAGGAAGAATAATAAAGGAAAGAATTGGCGGTCCATCATTTGCGAGCTTTATGACTTCATTTATAGGGATTGGAACTGTGTACATAATGGTTTTTGTGGTTATAGTAATGATGCTTATTCGATCAATCAATAAAGAAGACGACGAATCCCCTGTGGCTCGTACTGGAGACCAGGTTGATCACGCTACAAGCTCAACCGTTGAGGCTGAAAGCAGAGAGCACAGATCTGGAGAAAAGGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005911 GO:0006457 GO:0006950 GO:0008150 GO:0009505 GO:0009506 GO:0009987 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0030054 GO:0030312 GO:0031090 GO:0033554 GO:0034976 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0055044 GO:0071944 GO:0098588 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Thioredoxin Thioredoxin_6

Protein Analysis

452

Amino Acids

50.6

Weight (kDa)

4.94

Isoelectric Point (pI)

42.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	35 - 151	6.7e-20	Thioredoxin
Thioredoxin_6	PF13848	192 - 367	9.2e-12	Thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35620
fragaria_vesca	FvH4_5g30970 FvH4_5g30970
malus_domestica	MD08G1220700.v1.1 MD09G1249700.v1.1 MD15G1411400.v1.1
prunus_persica	Prupe.1G557400_v2.0.a1
pyrus_communis	pycom08g19150 pycom15g36620
rosa_chinensis	RchiOBHm_Chr7g0226321 RchiOBHm_Chr7g0226361
rosa_laevigata	RLG00000001746 RLG00000001751
rosa_multiflora	Rmu_sc0001044.1_g000026 Rmu_sc0004322.1_g000024 Rmu_sc0004426.1_g000003 Rmu_sc0004426.1_g000004
rosa_roxburghii	Rroxscaffold_3G00233030 Rroxscaffold_5G00366610
rosa_rugosa	Rorug07G0236600
rosa_samantha	Rh7AG383600 Rh7BG370400 Rh7CG403200 Rh7DG382000
rosa_wichuraiana	Rw7G032130 Rw7G032150 Rw7G032210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	752
AasI	GACNNNNNNGTC	1 cut(s)	802
AccII	CGCG	1 cut(s)	6
AciI	CCGC	8 cut(s)	74, 89, 135, 144, 186, 425, 899, 1140
AclWI	GGATC	3 cut(s)	317, 947, 1023
AcoI	YGGCCR	2 cut(s)	187, 774
AcsI	RAATTY	2 cut(s)	284, 503
AcuI	CTGAAG	1 cut(s)	467
AcyI	GRCGYC	1 cut(s)	77
AfaI	GTAC	5 cut(s)	9, 590, 1031, 1193, 1273
AfiI	CCNNNNNNNGG	1 cut(s)	185
AgsI	TTSAA	5 cut(s)	247, 336, 482, 556, 728
AjnI	CCWGG	4 cut(s)	179, 416, 510, 1281
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AluBI	AGCT	6 cut(s)	112, 224, 299, 461, 1158, 1302
AluI	AGCT	6 cut(s)	112, 224, 299, 461, 1158, 1302
Alw21I	GWGCWC	2 cut(s)	114, 1332
Alw26I	GTCTC	4 cut(s)	206, 788, 1090, 1272
AlwI	GGATC	3 cut(s)	317, 947, 1023
AlwNI	CAGNNNCTG	2 cut(s)	464, 491
AoxI	GGCC	2 cut(s)	187, 774
ApeKI	GCWGC	8 cut(s)	47, 65, 68, 71, 221, 224, 458, 515
ApoI	RAATTY	2 cut(s)	284, 503
ArsI	GACNNNNNNTTYG	2 cut(s)	908, 940
AspLEI	GCGC	3 cut(s)	6, 31, 705
AspS9I	GGNCC	3 cut(s)	413, 719, 1142
AsuHPI	GGTGA	1 cut(s)	280
AvaII	GGWCC	3 cut(s)	413, 719, 1142
BalI	TGGCCA	1 cut(s)	776
BanII	GRGCYC	1 cut(s)	114
BbsI	GAAGAC	3 cut(s)	960, 992, 1254
Bbv12I	GWGCWC	2 cut(s)	114, 1332
BbvI	GCAGC	8 cut(s)	59, 77, 80, 83, 211, 233, 470, 502
BccI	CCATC	2 cut(s)	146, 1153
BcgI	CGANNNNNNTGC	6 cut(s)	39, 60, 73, 94, 591, 625
BciT130I	CCWGG	4 cut(s)	181, 418, 512, 1283
BciVI	GTATCC	1 cut(s)	1097
BclI	TGATCA	1 cut(s)	1288
BcoDI	GTCTC	4 cut(s)	206, 788, 1090, 1272
BfaI	CTAG	1 cut(s)	53
BfmI	CTRYAG	1 cut(s)	294
BfoI	RGCGCY	1 cut(s)	32
BfuI	GTATCC	1 cut(s)	1097
BglII	AGATCT	1 cut(s)	1334
Bme1390I	CCNGG	4 cut(s)	181, 418, 512, 1283
Bme18I	GGWCC	3 cut(s)	413, 719, 1142
BmgT120I	GGNCC	3 cut(s)	413, 719, 1142
BmiI	GGNNCC	4 cut(s)	415, 666, 721, 976
BmrFI	CCNGG	4 cut(s)	181, 418, 512, 1283
BmrI	ACTGGG	1 cut(s)	248
BmsI	GCATC	5 cut(s)	208, 686, 911, 1085, 1212
BmuI	ACTGGG	1 cut(s)	248
BpiI	GAAGAC	3 cut(s)	960, 992, 1254
BpmI	CTGGAG	1 cut(s)	1296
BpuEI	CTTGAG	1 cut(s)	1065
BsaHI	GRCGYC	1 cut(s)	77
BsaI	GGTCTC	1 cut(s)	1272
BsaJI	CCNNGG	4 cut(s)	147, 179, 399, 416
BsaXI	ACNNNNNCTCC	2 cut(s)	1269, 1299
Bsc4I	CCNNNNNNNGG	1 cut(s)	185
Bse118I	RCCGGY	1 cut(s)	61
Bse1I	ACTGG	2 cut(s)	254, 1279
Bse3DI	GCAATG	1 cut(s)	352
BseBI	CCWGG	4 cut(s)	181, 418, 512, 1283
BseDI	CCNNGG	4 cut(s)	147, 179, 399, 416
BseGI	GGATG	4 cut(s)	223, 677, 685, 862
BseLI	CCNNNNNNNGG	1 cut(s)	185
BseMI	GCAATG	1 cut(s)	352
BseMII	CTCAG	1 cut(s)	224
BseNI	ACTGG	2 cut(s)	254, 1279
BseRI	GAGGAG	3 cut(s)	198, 746, 1040
BseXI	GCAGC	8 cut(s)	59, 77, 80, 83, 211, 233, 470, 502
BseYI	CCCAGC	1 cut(s)	239
Bsh1236I	CGCG	1 cut(s)	6
BshFI	GGCC	2 cut(s)	189, 776
BsiHKAI	GWGCWC	2 cut(s)	114, 1332
BsiSI	CCGG	1 cut(s)	62
BsiWI	CGTACG	1 cut(s)	7
BslFI	GGGAC	3 cut(s)	112, 705, 929
BslI	CCNNNNNNNGG	1 cut(s)	185
BsmAI	GTCTC	4 cut(s)	206, 788, 1090, 1272
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	3 cut(s)	112, 705, 929
BsnI	GGCC	2 cut(s)	189, 776
Bso31I	GGTCTC	1 cut(s)	1272
Bsp1286I	GDGCHC	2 cut(s)	114, 1332
Bsp1407I	TGTACA	1 cut(s)	1191
Bsp143I	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
Bsp19I	CCATGG	1 cut(s)	399
BspACI	CCGC	8 cut(s)	74, 89, 135, 144, 186, 425, 899, 1140
BspANI	GGCC	2 cut(s)	189, 776
BspCNI	CTCAG	1 cut(s)	223
BspFNI	CGCG	1 cut(s)	6
BspLI	GGNNCC	4 cut(s)	415, 666, 721, 976
BspPI	GGATC	3 cut(s)	317, 947, 1023
BspTNI	GGTCTC	1 cut(s)	1272
BsrDI	GCAATG	1 cut(s)	352
BsrFI	RCCGGY	1 cut(s)	61
BsrGI	TGTACA	1 cut(s)	1191
BsrI	ACTGG	2 cut(s)	254, 1279
BssAI	RCCGGY	1 cut(s)	61
BssECI	CCNNGG	4 cut(s)	147, 179, 399, 416
BssMI	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
BssNI	GRCGYC	1 cut(s)	77
BssT1I	CCWWGG	2 cut(s)	147, 399
Bst2UI	CCWGG	4 cut(s)	181, 418, 512, 1283
Bst4CI	ACNGT	8 cut(s)	103, 381, 392, 948, 1039, 1092, 1189, 1309
Bst6I	CTCTTC	2 cut(s)	723, 855
BstACI	GRCGYC	1 cut(s)	77
BstAUI	TGTACA	1 cut(s)	1191
BstC8I	GCNNGC	5 cut(s)	63, 197, 237, 520, 1156
BstDEI	CTNAG	2 cut(s)	210, 870
BstDSI	CCRYGG	1 cut(s)	399
BstF5I	GGATG	4 cut(s)	223, 677, 685, 862
BstFNI	CGCG	1 cut(s)	6
BstH2I	RGCGCY	1 cut(s)	32
BstHHI	GCGC	3 cut(s)	6, 31, 705
BstKTI	GATC	7 cut(s)	325, 942, 1018, 1072, 1235, 1291, 1337
BstMAI	GTCTC	4 cut(s)	206, 788, 1090, 1272
BstMBI	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
BstMWI	GCNNNNNNNGC	5 cut(s)	71, 74, 143, 192, 905
BstNI	CCWGG	4 cut(s)	181, 418, 512, 1283
BstSCI	CCNGG	4 cut(s)	179, 416, 510, 1281
BstSFI	CTRYAG	1 cut(s)	294
BstUI	CGCG	1 cut(s)	6
BstV1I	GCAGC	8 cut(s)	59, 77, 80, 83, 211, 233, 470, 502
BstV2I	GAAGAC	3 cut(s)	960, 992, 1254
BstX2I	RGATCY	1 cut(s)	1334
BstXI	CCANNNNNNTGG	1 cut(s)	518
BstYI	RGATCY	1 cut(s)	1334
BsuI	GTATCC	1 cut(s)	1097
BsuRI	GGCC	2 cut(s)	189, 776
BtgI	CCRYGG	1 cut(s)	399
BtsCI	GGATG	4 cut(s)	223, 677, 685, 862
BtsI	GCAGTG	1 cut(s)	190
BtsIMutI	CAGTG	2 cut(s)	190, 953
Cac8I	GCNNGC	5 cut(s)	63, 197, 237, 520, 1156
CaiI	CAGNNNCTG	2 cut(s)	464, 491
CfoI	GCGC	3 cut(s)	6, 31, 705
Cfr10I	RCCGGY	1 cut(s)	61
Cfr13I	GGNCC	3 cut(s)	413, 719, 1142
CseI	GACGC	2 cut(s)	66, 188
CsiI	ACCWGGT	1 cut(s)	1281
Csp6I	GTAC	5 cut(s)	8, 589, 1030, 1192, 1272
CviAII	CATG	4 cut(s)	359, 400, 603, 778
CviQI	GTAC	5 cut(s)	8, 589, 1030, 1192, 1272
DdeI	CTNAG	2 cut(s)	210, 870
DpnI	GATC	7 cut(s)	324, 941, 1017, 1071, 1234, 1290, 1336
DpnII	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
DraI	TTTAAA	1 cut(s)	891
DrdI	GACNNNNNNGTC	1 cut(s)	802
DseDI	GACNNNNNNGTC	1 cut(s)	802
EaeI	YGGCCR	2 cut(s)	187, 774
Eam1104I	CTCTTC	2 cut(s)	723, 855
EarI	CTCTTC	2 cut(s)	723, 855
EciI	GGCGGA	2 cut(s)	124, 133
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	2 cut(s)	147, 399
Eco24I	GRGCYC	1 cut(s)	114
Eco31I	GGTCTC	1 cut(s)	1272
Eco32I	GATATC	1 cut(s)	39
Eco47I	GGWCC	3 cut(s)	413, 719, 1142
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	1 cut(s)	467
EcoICRI	GAGCTC	1 cut(s)	112
EcoRII	CCWGG	4 cut(s)	179, 416, 510, 1281
EcoRV	GATATC	1 cut(s)	39
EcoT14I	CCWWGG	2 cut(s)	147, 399
EcoT22I	ATGCAT	1 cut(s)	364
EcoT38I	GRGCYC	1 cut(s)	114
ErhI	CCWWGG	2 cut(s)	147, 399
Esp3I	CGTCTC	1 cut(s)	206
FaeI	CATG	4 cut(s)	362, 403, 606, 781
FaqI	GGGAC	3 cut(s)	112, 705, 929
FatI	CATG	4 cut(s)	358, 399, 602, 777
FbaI	TGATCA	1 cut(s)	1288
FokI	GGATG	4 cut(s)	230, 664, 672, 869
FriOI	GRGCYC	1 cut(s)	114
FspBI	CTAG	1 cut(s)	53
GlaI	GCGC	3 cut(s)	5, 30, 704
GsaI	CCCAGC	1 cut(s)	243
GsuI	CTGGAG	1 cut(s)	1296
HaeII	RGCGCY	1 cut(s)	32
HaeIII	GGCC	2 cut(s)	189, 776
HapII	CCGG	1 cut(s)	62
HgaI	GACGC	2 cut(s)	66, 188
HhaI	GCGC	3 cut(s)	6, 31, 705
Hin1I	GRCGYC	1 cut(s)	77
Hin1II	CATG	4 cut(s)	362, 403, 606, 781
Hin6I	GCGC	3 cut(s)	4, 29, 703
HinP1I	GCGC	3 cut(s)	4, 29, 703
HinfI	GANTC	8 cut(s)	56, 119, 131, 428, 482, 488, 560, 1256
HpaII	CCGG	1 cut(s)	62
HphI	GGTGA	1 cut(s)	280
Hpy166II	GTNNAC	2 cut(s)	94, 1192
Hpy188I	TCNGA	6 cut(s)	36, 369, 447, 487, 631, 1051
Hpy188III	TCNNGA	3 cut(s)	464, 914, 1338
Hpy8I	GTNNAC	2 cut(s)	94, 1192
Hpy99I	CGWCG	4 cut(s)	82, 85, 119, 1256
HpyAV	CCTTC	4 cut(s)	568, 592, 692, 1106
HpyCH4III	ACNGT	8 cut(s)	103, 381, 392, 948, 1039, 1092, 1189, 1309
HpyCH4IV	ACGT	1 cut(s)	692
HpyCH4V	TGCA	8 cut(s)	195, 221, 266, 362, 388, 458, 617, 908
HpyF10VI	GCNNNNNNNGC	5 cut(s)	71, 74, 143, 192, 905
HpyF3I	CTNAG	2 cut(s)	210, 870
HpySE526I	ACGT	1 cut(s)	692
Hsp92I	GRCGYC	1 cut(s)	77
Hsp92II	CATG	4 cut(s)	362, 403, 606, 781
HspAI	GCGC	3 cut(s)	4, 29, 703
KroI	GCCGGC	1 cut(s)	61
KroNI	GCCGGC	1 cut(s)	63
Ksp22I	TGATCA	1 cut(s)	1288
Kzo9I	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
LmnI	GCTCC	3 cut(s)	109, 466, 974
Lsp1109I	GCAGC	8 cut(s)	59, 77, 80, 83, 211, 233, 470, 502
LweI	GCATC	5 cut(s)	208, 686, 911, 1085, 1212
MabI	ACCWGGT	1 cut(s)	1281
MaeI	CTAG	1 cut(s)	53
MaeII	ACGT	1 cut(s)	692
MalI	GATC	7 cut(s)	324, 941, 1017, 1071, 1234, 1290, 1336
MboI	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
MflI	RGATCY	1 cut(s)	1334
MhlI	GDGCHC	2 cut(s)	114, 1332
MlsI	TGGCCA	1 cut(s)	776
MluCI	AATT	4 cut(s)	284, 503, 828, 1134
MluNI	TGGCCA	1 cut(s)	776
MlyI	GAGTC	2 cut(s)	482, 569
MmeI	TCCRAC	5 cut(s)	87, 147, 195, 392, 713
MnlI	CCTC	8 cut(s)	219, 301, 383, 724, 847, 1018, 1057, 1306
Mox20I	TGGCCA	1 cut(s)	776
Mph1103I	ATGCAT	1 cut(s)	364
MroNI	GCCGGC	1 cut(s)	61
MscI	TGGCCA	1 cut(s)	776
MseI	TTAA	3 cut(s)	273, 800, 890
MslI	CAYNNNNRTG	1 cut(s)	357
Msp20I	TGGCCA	1 cut(s)	776
MspA1I	CMGCKG	3 cut(s)	74, 224, 899
MspI	CCGG	1 cut(s)	62
MspR9I	CCNGG	4 cut(s)	181, 418, 512, 1283
MvaI	CCWGG	4 cut(s)	181, 418, 512, 1283
MvnI	CGCG	1 cut(s)	6
MwoI	GCNNNNNNNGC	5 cut(s)	71, 74, 143, 192, 905
NaeI	GCCGGC	1 cut(s)	63
NcoI	CCATGG	1 cut(s)	399
NdeII	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
NgoMIV	GCCGGC	1 cut(s)	61
NlaIII	CATG	4 cut(s)	362, 403, 606, 781
NlaIV	GGNNCC	4 cut(s)	415, 666, 721, 976
NsiI	ATGCAT	1 cut(s)	364
PdiI	GCCGGC	1 cut(s)	63
PfeI	GAWTC	6 cut(s)	56, 119, 131, 428, 482, 1256
Pfl23II	CGTACG	1 cut(s)	7
PflFI	GACNNNGTC	1 cut(s)	155
PleI	GAGTC	2 cut(s)	482, 568
PpsI	GAGTC	2 cut(s)	482, 568
PsiI	TTATAA	1 cut(s)	752
Psp124BI	GAGCTC	1 cut(s)	114
Psp6I	CCWGG	4 cut(s)	179, 416, 510, 1281
PspFI	CCCAGC	1 cut(s)	239
PspGI	CCWGG	4 cut(s)	179, 416, 510, 1281
PspLI	CGTACG	1 cut(s)	7
PspN4I	GGNNCC	4 cut(s)	415, 666, 721, 976
PspPI	GGNCC	3 cut(s)	413, 719, 1142
PstNI	CAGNNNCTG	2 cut(s)	464, 491
PsuI	RGATCY	1 cut(s)	1334
PsyI	GACNNNGTC	1 cut(s)	155
PvuII	CAGCTG	1 cut(s)	224
RsaI	GTAC	5 cut(s)	9, 590, 1031, 1193, 1273
RsaNI	GTAC	5 cut(s)	8, 589, 1030, 1192, 1272
RseI	CAYNNNNRTG	1 cut(s)	357
SacI	GAGCTC	1 cut(s)	114
SaqAI	TTAA	3 cut(s)	273, 800, 890
Sau3AI	GATC	7 cut(s)	322, 939, 1015, 1069, 1232, 1288, 1334
Sau96I	GGNCC	3 cut(s)	413, 719, 1142
SchI	GAGTC	2 cut(s)	482, 569
ScrFI	CCNGG	4 cut(s)	181, 418, 512, 1283
SduI	GDGCHC	2 cut(s)	114, 1332
SexAI	ACCWGGT	1 cut(s)	1281
SfaNI	GCATC	5 cut(s)	208, 686, 911, 1085, 1212
SfcI	CTRYAG	1 cut(s)	294
SinI	GGWCC	3 cut(s)	413, 719, 1142
SmiMI	CAYNNNNRTG	1 cut(s)	357
SmlI	CTYRAG	1 cut(s)	1080
SmoI	CTYRAG	1 cut(s)	1080
Sse9I	AATT	4 cut(s)	284, 503, 828, 1134
SsiI	CCGC	8 cut(s)	74, 89, 135, 144, 186, 425, 899, 1140
SspI	AATATT	1 cut(s)	886
SspMI	CTAG	1 cut(s)	53
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	4 cut(s)	179, 416, 510, 1281
StyI	CCWWGG	2 cut(s)	147, 399
TaaI	ACNGT	8 cut(s)	103, 381, 392, 948, 1039, 1092, 1189, 1309
TaiI	ACGT	1 cut(s)	695
TaqI	TCGA	7 cut(s)	114, 129, 301, 315, 572, 1018, 1231
TasI	AATT	4 cut(s)	284, 503, 828, 1134
TatI	WGTACW	3 cut(s)	588, 1029, 1191
TauI	GCSGC	3 cut(s)	77, 189, 902
TfiI	GAWTC	6 cut(s)	56, 119, 131, 428, 482, 1256
Tru1I	TTAA	3 cut(s)	273, 800, 890
Tru9I	TTAA	3 cut(s)	273, 800, 890
TscAI	CASTG	2 cut(s)	197, 953
TseI	GCWGC	8 cut(s)	47, 65, 68, 71, 221, 224, 458, 515
TspDTI	ATGAA	4 cut(s)	674, 834, 873, 1158
TspRI	CASTG	2 cut(s)	197, 953
Tth111I	GACNNNGTC	1 cut(s)	155
VpaK11BI	GGWCC	3 cut(s)	413, 719, 1142
XapI	RAATTY	2 cut(s)	284, 503
XcmI	CCANNNNNNNNNTGG	1 cut(s)	145
XspI	CTAG	1 cut(s)	53
Zsp2I	ATGCAT	1 cut(s)	364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.