Rw7G032150

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006094
Physical Location & Seq
Reverse (-)
46515067 .. 46516938
1872 bp
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UTR
Exon/CDS
Intron
Rw7G032150.1

Sequence Viewer

Length: 843 bp
ATGCGCGGCATGGGTGGCTTAACGATGTGGTTCTGGGCGCTGACGTCAGCAGCAGCTATATTGGAGTCGTCAGTGTCGCCGTCGTCGTCTTGGGTGGCTGACGGGACGGTGCTGGAGCTGGACGATTCCAACTTCGAATCGGCGATTTCAGCGCTCGACTTGGTCTTGGTGGACTTCCACGCCCCATGGTGCCCCCACAGCAAGAGTCTCTCTCCTCAGTTGGACGCGGCTGCGCCGATGCTTGCTTCATTGAAGAAACCAATAGTGATAGCAAAAGTTGATGCTGACAAGTCTACATCTCTAGCTTGCAAATACGGAGTCGATGGATATCCTACGCTGAAGCTCTTTATACATGGTGTCCCCCAAGAGTATATGGGGCCAAGGAAAGCCGATTTGCTTGTTCGTTATCTCAAGAAATTTGCAGCTCCCGATGTTACCCTACTTGATTCAGACTCTGCTATTGCTGATTTTGTTGGAGCGGCAGGCACATTCTTCCCCATATATGTTGGTTTTGGCTTAGATGAGTCCGTGATATCGAAGTTGGCATTGAAGTACAAAAGGAAGGCCTGGTTTTCGGTTGCAAAGGATTTCTCTGAGGATGTAATGCTATTGTATGATTTTGACAAGGTTCCTGCTCTGGTATCCCTTCATCCAAGTTGCGATGAGCGCTACGTCTTTTACGGCCCCTTTGAAGAGGAATTCTTGGAAGATTTCATTAAACAAAGTTTGTTACCTTTGGCCATGCCCATCAACCATGACACATTAAAATCATTGGACGATGATGAAAGGAAAATTATGTTGACAATTGTGGAGGATGAGGATGAAGAAAGTCAAAGAACTTGA

Protein Analysis

280

Amino Acids

31.02

Weight (kDa)

4.62

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 37 - 139 1.7e-22 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 189
AccBSI CCGCTC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 293
AccII CGCG 2 cut(s) 6, 227
AciI CCGC 3 cut(s) 6, 227, 479
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 2 cut(s) 416, 698
AcuI CTGAAG 1 cut(s) 359
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 1 cut(s) 554
AfeI AGCGCT 2 cut(s) 153, 668
AgsI TTSAA 3 cut(s) 253, 550, 692
AjnI CCWGG 1 cut(s) 566
AluBI AGCT 5 cut(s) 56, 118, 305, 343, 425
AluI AGCT 5 cut(s) 56, 118, 305, 343, 425
Alw26I GTCTC 1 cut(s) 212
AlwNI CAGNNNCTG 1 cut(s) 455
Aor51HI AGCGCT 2 cut(s) 153, 668
AoxI GGCC 4 cut(s) 377, 564, 682, 738
ApeKI GCWGC 4 cut(s) 50, 53, 230, 422
ApoI RAATTY 2 cut(s) 416, 698
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 5 cut(s) 6, 40, 154, 235, 669
AspS9I GGNCC 2 cut(s) 377, 683
AsuII TTCGAA 1 cut(s) 135
BaeGI GKGCMC 1 cut(s) 194
BalI TGGCCA 1 cut(s) 740
BanI GGYRCC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 62, 65, 217, 434
BccI CCATC 2 cut(s) 317, 755
BceAI ACGGC 2 cut(s) 64, 697
BciT130I CCWGG 1 cut(s) 568
BciVI GTATCC 1 cut(s) 652
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 1 cut(s) 302
BfoI RGCGCY 3 cut(s) 41, 155, 670
BfuI GTATCC 1 cut(s) 652
BisI GCNGC 7 cut(s) 7, 51, 54, 228, 231, 423, 480
BlsI GCNGC 7 cut(s) 8, 52, 55, 229, 232, 424, 481
Bme1390I CCNGG 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 377, 683
BmiI GGNNCC 4 cut(s) 191, 378, 630, 685
BmrFI CCNGG 1 cut(s) 568
BmsI GCATC 2 cut(s) 228, 271
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpmI CTGGAG 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 185, 380
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 1 cut(s) 568
BseDI CCNNGG 2 cut(s) 185, 380
BseGI GGATG 4 cut(s) 604, 649, 820, 826
BseJI GATNNNNATC 1 cut(s) 327
BseMII CTCAG 2 cut(s) 230, 585
BseRI GAGGAG 1 cut(s) 204
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 4 cut(s) 62, 65, 217, 434
Bsh1236I CGCG 2 cut(s) 6, 227
BshFI GGCC 4 cut(s) 379, 566, 684, 740
BshNI GGYRCC 1 cut(s) 189
BslFI GGGAC 2 cut(s) 118, 344
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 2 cut(s) 118, 344
BsnI GGCC 4 cut(s) 379, 566, 684, 740
Bsp119I TTCGAA 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 194
Bsp19I CCATGG 1 cut(s) 185
BspACI CCGC 3 cut(s) 6, 227, 479
BspANI GGCC 4 cut(s) 379, 566, 684, 740
BspCNI CTCAG 2 cut(s) 229, 586
BspFNI CGCG 2 cut(s) 6, 227
BspLI GGNNCC 4 cut(s) 191, 378, 630, 685
BspT104I TTCGAA 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 189
BsrBI CCGCTC 1 cut(s) 479
BssECI CCNNGG 2 cut(s) 185, 380
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 2 cut(s) 185, 380
Bst2UI CCWGG 1 cut(s) 568
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 243, 307, 484
BstDEI CTNAG 3 cut(s) 216, 517, 594
BstDSI CCRYGG 1 cut(s) 185
BstF5I GGATG 4 cut(s) 604, 649, 820, 826
BstFNI CGCG 2 cut(s) 6, 227
BstH2I RGCGCY 3 cut(s) 41, 155, 670
BstHHI GCGC 5 cut(s) 6, 40, 154, 235, 669
BstMAI GTCTC 1 cut(s) 212
BstMWI GCNNNNNNNGC 4 cut(s) 15, 149, 198, 666
BstNI CCWGG 1 cut(s) 568
BstSCI CCNGG 1 cut(s) 566
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 2 cut(s) 6, 227
BstV1I GCAGC 4 cut(s) 62, 65, 217, 434
BsuI GTATCC 1 cut(s) 652
BsuRI GGCC 4 cut(s) 379, 566, 684, 740
BtgI CCRYGG 1 cut(s) 185
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 604, 649, 820, 826
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 243, 307, 484
CaiI CAGNNNCTG 1 cut(s) 455
CfoI GCGC 5 cut(s) 6, 40, 154, 235, 669
Cfr13I GGNCC 2 cut(s) 377, 683
CseI GACGC 1 cut(s) 233
Csp6I GTAC 1 cut(s) 553
CviAII CATG 5 cut(s) 10, 186, 353, 742, 755
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 3 cut(s) 216, 517, 594
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Eco130I CCWWGG 2 cut(s) 185, 380
Eco147I AGGCCT 1 cut(s) 566
Eco32I GATATC 2 cut(s) 329, 534
Eco47III AGCGCT 2 cut(s) 153, 668
Eco57I CTGAAG 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 566
EcoRV GATATC 2 cut(s) 329, 534
EcoT14I CCWWGG 2 cut(s) 185, 380
ErhI CCWWGG 2 cut(s) 185, 380
FaeI CATG 5 cut(s) 13, 189, 356, 745, 758
FaqI GGGAC 2 cut(s) 118, 344
FatI CATG 5 cut(s) 9, 185, 352, 741, 754
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 7 cut(s) 7, 51, 54, 228, 231, 423, 480
FokI GGATG 4 cut(s) 611, 636, 827, 833
Fsp4HI GCNGC 7 cut(s) 7, 51, 54, 228, 231, 423, 480
FspBI CTAG 1 cut(s) 302
GlaI GCGC 5 cut(s) 5, 39, 153, 234, 668
GluI GCNGC 7 cut(s) 7, 51, 54, 228, 231, 423, 480
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 3 cut(s) 41, 155, 670
HaeIII GGCC 4 cut(s) 379, 566, 684, 740
HgaI GACGC 1 cut(s) 233
HhaI GCGC 5 cut(s) 6, 40, 154, 235, 669
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 5 cut(s) 13, 189, 356, 745, 758
Hin6I GCGC 5 cut(s) 4, 38, 152, 233, 667
HinP1I GCGC 5 cut(s) 4, 38, 152, 233, 667
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HinfI GANTC 8 cut(s) 65, 125, 137, 205, 318, 446, 452, 524
Hpy166II GTNNAC 3 cut(s) 172, 294, 801
Hpy188I TCNGA 2 cut(s) 451, 595
Hpy188III TCNNGA 2 cut(s) 412, 428
Hpy8I GTNNAC 3 cut(s) 172, 294, 801
Hpy99I CGWCG 2 cut(s) 85, 88
HpyAV CCTTC 2 cut(s) 556, 656
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 44, 672
HpyCH4V TGCA 3 cut(s) 309, 422, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 149, 198, 666
HpyF3I CTNAG 3 cut(s) 216, 517, 594
HpySE526I ACGT 2 cut(s) 44, 672
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 5 cut(s) 13, 189, 356, 745, 758
HspAI GCGC 5 cut(s) 4, 38, 152, 233, 667
LmnI GCTCC 3 cut(s) 115, 430, 476
LpnPI CCDG 8 cut(s) 19, 98, 104, 468, 553, 580, 623, 645
Lsp1109I GCAGC 4 cut(s) 62, 65, 217, 434
LweI GCATC 2 cut(s) 228, 271
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 44, 672
MaeIII GTNAC 2 cut(s) 433, 729
MbiI CCGCTC 1 cut(s) 479
MboII GAAGA 5 cut(s) 265, 484, 704, 719, 836
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 4 cut(s) 416, 698, 792, 804
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 5 cut(s) 74, 214, 327, 446, 533
MmeI TCCRAC 3 cut(s) 153, 201, 454
MnlI CCTC 5 cut(s) 225, 589, 688, 805, 811
Mox20I TGGCCA 1 cut(s) 740
MscI TGGCCA 1 cut(s) 740
MseI TTAA 3 cut(s) 20, 717, 764
Msp20I TGGCCA 1 cut(s) 740
MspR9I CCNGG 1 cut(s) 568
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 1 cut(s) 568
MvnI CGCG 2 cut(s) 6, 227
MwoI GCNNNNNNNGC 4 cut(s) 15, 149, 198, 666
NcoI CCATGG 1 cut(s) 185
NlaIII CATG 5 cut(s) 13, 189, 356, 745, 758
NlaIV GGNNCC 4 cut(s) 191, 378, 630, 685
NspV TTCGAA 1 cut(s) 135
PceI AGGCCT 1 cut(s) 566
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PfeI GAWTC 3 cut(s) 125, 137, 446
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 7 cut(s) 8, 52, 55, 229, 232, 424, 481
PleI GAGTC 5 cut(s) 73, 213, 326, 446, 532
PpsI GAGTC 5 cut(s) 73, 213, 326, 446, 532
Psp6I CCWGG 1 cut(s) 566
PspGI CCWGG 1 cut(s) 566
PspN4I GGNNCC 4 cut(s) 191, 378, 630, 685
PspPI GGNCC 2 cut(s) 377, 683
PstNI CAGNNNCTG 1 cut(s) 455
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SaqAI TTAA 3 cut(s) 20, 717, 764
SatI GCNGC 7 cut(s) 7, 51, 54, 228, 231, 423, 480
Sau96I GGNCC 2 cut(s) 377, 683
SchI GAGTC 5 cut(s) 74, 214, 327, 446, 533
ScrFI CCNGG 1 cut(s) 568
SduI GDGCHC 1 cut(s) 194
SetI ASST 9 cut(s) 47, 58, 120, 307, 345, 427, 630, 675, 736
SfaNI GCATC 2 cut(s) 228, 271
SfuI TTCGAA 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 4 cut(s) 416, 698, 792, 804
SseBI AGGCCT 1 cut(s) 566
SsiI CCGC 3 cut(s) 6, 227, 479
SspMI CTAG 1 cut(s) 302
StuI AGGCCT 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 566
StyI CCWWGG 2 cut(s) 185, 380
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 47, 675
TaqI TCGA 4 cut(s) 135, 156, 321, 536
TasI AATT 4 cut(s) 416, 698, 792, 804
TatI WGTACW 1 cut(s) 552
TauI GCSGC 3 cut(s) 9, 230, 482
TfiI GAWTC 3 cut(s) 125, 137, 446
Tru1I TTAA 3 cut(s) 20, 717, 764
Tru9I TTAA 3 cut(s) 20, 717, 764
TscAI CASTG 1 cut(s) 78
TseI GCWGC 4 cut(s) 50, 53, 230, 422
TspDTI ATGAA 5 cut(s) 237, 638, 703, 798, 837
TspGWI ACGGA 2 cut(s) 330, 517
TspRI CASTG 1 cut(s) 78
Tth111I GACNNNGTC 1 cut(s) 161
XapI RAATTY 2 cut(s) 416, 698
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 302
ZraI GACGTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.