RchiOBHm_Chr7g0226361

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006094
Physical Location & Seq
Reverse (-)
49562384 .. 49566710
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20275

Sequence Viewer

Length: 834 bp
ATGGGTGGCTTAACGGTGTGGTTCTGGGCGCTGACGTCAGCAGCAGCTATATTGGAGGCGTCAGTGTCGTCGTCGTCGTCTTGGGTGGCTGACGGGACGGTGCTGGAGCTGGACGATTCCAACTTCGAATCGGCGATTTCAGCGCTCGACTTGGTCTTGGTGGACTTCCACGCCCCATGGTGCCCCCACAGCAAGAGTCTCTCTCCTCAGTTGGACGCGGCTGCGCCGATGCTTGCTTCATTGAAGAAACCAATAGTGATAGCAAAAGTTGATGCTGACAAGTCTACATCTCTAGCTTGCAAATGCGGAGTCGATGGATATCCTACGCTGAAGCTCTTTATACATGGTGTCCCCCAAGAGTATATGGGGCCAAGGAAAGCCGATTTGCTTGTTCGTTATCTCAAGAAATTTGCAGCTCCCGATGTTACCCTACTTGATTCAGACTCTGCTATTGCTGATTTTGTTGGAGCGGCAGGCACATTCTTCCCCATATATGTTGGTTTTGGCTTAGATGAGTCCGTGATATCTAAGTTGGCATTGAAGTACAAAAGGAAGGCCTGGTTTTCGGTTGCAAAGGATTTCTCTGAGGATGTAATGCTGTTGTATGATTTTGACAACGTTCCTGCTCTGGTATCCCTTCATCCAAGTTGCGATGAGCGCTACGTCTTTTACGGCCCCTTTGAAGAGGAATTCTTGGAAGATTTCATTAAACAAAGTTTGTTACCTTTGGCCATGCCCATCAACCATGACACATTAAAATCATTGGACGATGATGAAAGGAAAATTATGTTGACAATTGTGGAGGATGAGGATGAAGAAAGTCAAAGAATTTGA

Protein Analysis

277

Amino Acids

30.56

Weight (kDa)

4.53

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 34 - 136 3.1e-22 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 180
AccBSI CCGCTC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 284
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 218, 306, 470
AclI AACGTT 1 cut(s) 618
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 3 cut(s) 407, 689, 828
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 2 cut(s) 35, 59
AfaI GTAC 1 cut(s) 545
AfeI AGCGCT 2 cut(s) 144, 659
AgsI TTSAA 3 cut(s) 244, 541, 683
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 5 cut(s) 47, 109, 296, 334, 416
AluI AGCT 5 cut(s) 47, 109, 296, 334, 416
Alw26I GTCTC 1 cut(s) 203
AlwNI CAGNNNCTG 1 cut(s) 446
Aor51HI AGCGCT 2 cut(s) 144, 659
AoxI GGCC 4 cut(s) 368, 555, 673, 729
ApeKI GCWGC 4 cut(s) 41, 44, 221, 413
ApoI RAATTY 3 cut(s) 407, 689, 828
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspLEI GCGC 4 cut(s) 31, 145, 226, 660
AspS9I GGNCC 2 cut(s) 368, 674
AsuII TTCGAA 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 180
BbvI GCAGC 4 cut(s) 53, 56, 208, 425
BccI CCATC 2 cut(s) 308, 746
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 1 cut(s) 559
BciVI GTATCC 1 cut(s) 643
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 293
BfoI RGCGCY 3 cut(s) 32, 146, 661
BfuI GTATCC 1 cut(s) 643
BisI GCNGC 6 cut(s) 42, 45, 219, 222, 414, 471
BlsI GCNGC 6 cut(s) 43, 46, 220, 223, 415, 472
Bme1390I CCNGG 1 cut(s) 559
BmgT120I GGNCC 2 cut(s) 368, 674
BmiI GGNNCC 3 cut(s) 182, 369, 676
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 2 cut(s) 219, 262
BplI GAGNNNNNCTC 2 cut(s) 187, 219
BpmI CTGGAG 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 386
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 2 cut(s) 35, 59
BsaJI CCNNGG 2 cut(s) 176, 371
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 2 cut(s) 176, 371
BseGI GGATG 4 cut(s) 595, 640, 811, 817
BseJI GATNNNNATC 1 cut(s) 318
BseMII CTCAG 2 cut(s) 221, 576
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 4 cut(s) 53, 56, 208, 425
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 4 cut(s) 370, 557, 675, 731
BshNI GGYRCC 1 cut(s) 180
BslFI GGGAC 2 cut(s) 109, 335
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 109, 335
BsnI GGCC 4 cut(s) 370, 557, 675, 731
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 185
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 3 cut(s) 218, 306, 470
BspANI GGCC 4 cut(s) 370, 557, 675, 731
BspCNI CTCAG 2 cut(s) 220, 577
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 3 cut(s) 182, 369, 676
BspT104I TTCGAA 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 180
BsrBI CCGCTC 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 176, 371
BssNI GRCGYC 2 cut(s) 35, 59
BssT1I CCWWGG 2 cut(s) 176, 371
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 16, 100
Bst6I CTCTTC 1 cut(s) 678
BstACI GRCGYC 2 cut(s) 35, 59
BstBI TTCGAA 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 234, 298, 475
BstDEI CTNAG 4 cut(s) 207, 508, 528, 585
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 4 cut(s) 595, 640, 811, 817
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 3 cut(s) 32, 146, 661
BstHHI GCGC 4 cut(s) 31, 145, 226, 660
BstMAI GTCTC 1 cut(s) 203
BstMWI GCNNNNNNNGC 3 cut(s) 140, 189, 657
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 4 cut(s) 53, 56, 208, 425
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 4 cut(s) 370, 557, 675, 731
BtgI CCRYGG 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 4 cut(s) 595, 640, 811, 817
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 234, 298, 475
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 4 cut(s) 31, 145, 226, 660
Cfr13I GGNCC 2 cut(s) 368, 674
CseI GACGC 2 cut(s) 48, 224
Csp6I GTAC 1 cut(s) 544
CviAII CATG 4 cut(s) 177, 344, 733, 746
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 4 cut(s) 207, 508, 528, 585
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 176, 371
Eco147I AGGCCT 1 cut(s) 557
Eco32I GATATC 2 cut(s) 320, 525
Eco47III AGCGCT 2 cut(s) 144, 659
Eco57I CTGAAG 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 689
EcoRII CCWGG 1 cut(s) 557
EcoRV GATATC 2 cut(s) 320, 525
EcoT14I CCWWGG 2 cut(s) 176, 371
ErhI CCWWGG 2 cut(s) 176, 371
FaeI CATG 4 cut(s) 180, 347, 736, 749
FaqI GGGAC 2 cut(s) 109, 335
FatI CATG 4 cut(s) 176, 343, 732, 745
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 6 cut(s) 42, 45, 219, 222, 414, 471
FokI GGATG 4 cut(s) 602, 627, 818, 824
Fsp4HI GCNGC 6 cut(s) 42, 45, 219, 222, 414, 471
FspBI CTAG 1 cut(s) 293
GlaI GCGC 4 cut(s) 30, 144, 225, 659
GluI GCNGC 6 cut(s) 42, 45, 219, 222, 414, 471
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 3 cut(s) 32, 146, 661
HaeIII GGCC 4 cut(s) 370, 557, 675, 731
HgaI GACGC 2 cut(s) 48, 224
HhaI GCGC 4 cut(s) 31, 145, 226, 660
Hin1I GRCGYC 2 cut(s) 35, 59
Hin1II CATG 4 cut(s) 180, 347, 736, 749
Hin6I GCGC 4 cut(s) 29, 143, 224, 658
HinP1I GCGC 4 cut(s) 29, 143, 224, 658
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HinfI GANTC 7 cut(s) 116, 128, 196, 309, 437, 443, 515
Hpy166II GTNNAC 3 cut(s) 163, 285, 792
Hpy188I TCNGA 2 cut(s) 442, 586
Hpy188III TCNNGA 2 cut(s) 403, 419
Hpy8I GTNNAC 3 cut(s) 163, 285, 792
Hpy99I CGWCG 3 cut(s) 73, 76, 79
HpyAV CCTTC 2 cut(s) 547, 647
HpyCH4III ACNGT 2 cut(s) 16, 100
HpyCH4IV ACGT 3 cut(s) 35, 618, 663
HpyCH4V TGCA 3 cut(s) 300, 413, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 189, 657
HpyF3I CTNAG 4 cut(s) 207, 508, 528, 585
HpySE526I ACGT 3 cut(s) 35, 618, 663
Hsp92I GRCGYC 2 cut(s) 35, 59
Hsp92II CATG 4 cut(s) 180, 347, 736, 749
HspAI GCGC 4 cut(s) 29, 143, 224, 658
LmnI GCTCC 3 cut(s) 106, 421, 467
LpnPI CCDG 8 cut(s) 10, 89, 95, 459, 544, 571, 614, 636
Lsp1109I GCAGC 4 cut(s) 53, 56, 208, 425
LweI GCATC 2 cut(s) 219, 262
MaeI CTAG 1 cut(s) 293
MaeII ACGT 3 cut(s) 35, 618, 663
MaeIII GTNAC 2 cut(s) 424, 720
MbiI CCGCTC 1 cut(s) 470
MboII GAAGA 5 cut(s) 256, 475, 695, 710, 827
MfeI CAATTG 1 cut(s) 795
MhlI GDGCHC 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 5 cut(s) 407, 689, 783, 795, 828
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 4 cut(s) 205, 318, 437, 524
MmeI TCCRAC 3 cut(s) 144, 192, 445
MnlI CCTC 6 cut(s) 49, 216, 580, 679, 796, 802
Mox20I TGGCCA 1 cut(s) 731
MscI TGGCCA 1 cut(s) 731
MseI TTAA 3 cut(s) 11, 708, 755
Msp20I TGGCCA 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 559
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 3 cut(s) 140, 189, 657
NcoI CCATGG 1 cut(s) 176
NlaIII CATG 4 cut(s) 180, 347, 736, 749
NlaIV GGNNCC 3 cut(s) 182, 369, 676
NspV TTCGAA 1 cut(s) 126
PceI AGGCCT 1 cut(s) 557
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PfeI GAWTC 3 cut(s) 116, 128, 437
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 6 cut(s) 43, 46, 220, 223, 415, 472
PleI GAGTC 4 cut(s) 204, 317, 437, 523
PpsI GAGTC 4 cut(s) 204, 317, 437, 523
Psp1406I AACGTT 1 cut(s) 618
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 3 cut(s) 182, 369, 676
PspPI GGNCC 2 cut(s) 368, 674
PstNI CAGNNNCTG 1 cut(s) 446
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SaqAI TTAA 3 cut(s) 11, 708, 755
SatI GCNGC 6 cut(s) 42, 45, 219, 222, 414, 471
Sau96I GGNCC 2 cut(s) 368, 674
SchI GAGTC 4 cut(s) 205, 318, 437, 524
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 1 cut(s) 185
SetI ASST 9 cut(s) 38, 49, 111, 298, 336, 418, 621, 666, 727
SfaNI GCATC 2 cut(s) 219, 262
SfuI TTCGAA 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 5 cut(s) 407, 689, 783, 795, 828
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 3 cut(s) 218, 306, 470
SspMI CTAG 1 cut(s) 293
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 557
StyI CCWWGG 2 cut(s) 176, 371
TaaI ACNGT 2 cut(s) 16, 100
TaiI ACGT 3 cut(s) 38, 621, 666
TaqI TCGA 3 cut(s) 126, 147, 312
TasI AATT 5 cut(s) 407, 689, 783, 795, 828
TatI WGTACW 1 cut(s) 543
TauI GCSGC 2 cut(s) 221, 473
TfiI GAWTC 3 cut(s) 116, 128, 437
Tru1I TTAA 3 cut(s) 11, 708, 755
Tru9I TTAA 3 cut(s) 11, 708, 755
TscAI CASTG 1 cut(s) 69
TseI GCWGC 4 cut(s) 41, 44, 221, 413
TspDTI ATGAA 5 cut(s) 228, 629, 694, 789, 828
TspGWI ACGGA 1 cut(s) 508
TspRI CASTG 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 152
XapI RAATTY 3 cut(s) 407, 689, 828
XmiI GTMKAC 1 cut(s) 284
XspI CTAG 1 cut(s) 293
ZraI GACGTC 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.