Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Reverse (-)

34149450 .. 34157940

Gene Structure 8491 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1267300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGTCGAGGAGAGTGGAGGTGGATGGCCACCGTGAGACGGAGGACGACCGCGACGAGGAGGAGGAAGAGTACGAGGTGCAGGATCTGAGAGAACGAATCAAATCGTCGCGCGGGAGCCAGTTCGATCTAATCAAGAACGAGTTAGGACTCGGCGACGGGTCTTCGATTTGGCGGCGGCGGTTCAGTCGTCAGAGCCTGATCGACGGCGTCAGAGGGCTCTCCAAAGGACTCGTCATTCATCCTGACAACCGGTGGTATCGGGCATGGATGAAATTCATACTTTTATGGGCAGTGTACTCGTCCTTCTTCACACCTTTCGAGTTCGGGTTTTTCAGGGGTTTGGGGGAGAGACTCTTTATACTGGATGTAGTAGGACAGGTAGCATTCCTTGTAGACATTGTTTCGCAGTTCTTCATCGCCTACCGAGACAGTCACACCTATCGAATGGTCTATAAGCGCAACCGGATTGCTCTACGGTACTTGAAATCGAGTTTTATCGTTGACTTGCTTGGCTGTATGCCTTGGGATAACATCTACAAGGCTTGTGGGAGGAGAGAGGAAGTGAGGTATCTCCTATTGATAAGGTTATGTCGGGTGCGCAAAGTCACTAGGTTCTTTCATGACCTAGAAAAGGATATTCGTATCAATTATCTGTTTACCAGGATTATAAAGCTTATTGTCGTTGAACTCTACTGCACACATACAGCGGCCTGCATATTCTACTATTTGGCCACCACCCTGCCTGCTTCACAAGAAGGGTACACATGGATTGGAAGTTTGAAGTTAGGTGACTATAGTTATTCAAGCTTCAGGGACATTGACTTATGGAAGCGCTACACAACTTCTTTGTATTTTGCCATTGTAACCATGGCCACTGTTGGTTATGGAGATATTCATGCAGTCAATCTGAGGGAAATGATATTCATAATGGTTTATGTATCTTTTGACATGATTCTTGGAGCGTATTTGATTGGTAACATGACAGCGTTAATTGTAAAAGGATCAAAGACAGAAAAGTTCAGGGACAAAATGACAGATCTCATCAAATATATGAACAGAAATAGACTTGGGAAGGATCTCCGTAATCAAATCAAAGGTCACTTGCGTTTACAATATGAGAGTACCTACACGGAGGCTGCTGTTCTCCAGGAAATTCCTGCTTCAATTCGCTCTAAGATTTCACAAACTTTATATTTTCCATACATTGAAAGTGTTCCTCTCTTCAAGGGATGCTCGACAGAATTCATCAATCAGATTGTTATTAAACTGCATGAAGAATTTTTTCTCCCTGGAGAGGTGATCATGGAACCAGGAAACGTCGTGGATCAACTTTATTTCGTCTGTCATGGTGTGCTGGAGGAGGTGGGCATAGGGGAAGATGGAACAGAAGAAACTCTTGAGGTTTTGGAACCTAACAGCTCATTTGGAGAAATCTCCATTCTTTGCAACATTCCTCAACTTCATACAGTTAGGGTGTGTGAATTATGTAGACTTCTGCGACTTGATAAACAATCTTTTACAAGCATCCTTGACATATACTTTTATGACGGGCGGAAAATCTTGAACAACCTTTTAGAGGGTAAAGCACCTCGCATGAAACAACTAGAGTCAGACATTACATTTCATATTGGAAAGCAAGAAGCTGAGTTTGCTTTGAAGGTAAATAGTGCGGCTTACCACGGAGATCTATTTCAGCTAAAAGGTTTGATCCGAACTGGAGCTGATCCCAACAAGACAGATTATGATGGAAGGTCACCCTTGCATCTCGCTGCATTAAAAGGACATGAAGATATTACACTTTTCCTTATTCAGGAAGGAGTAGACATCAACATCAAAGACAATTTTGGGAACACACCCTTACTTGAAGCCATCAAGAACGGGAATGATCGAGTTTCTTCTTTACTTATCCAACAAGGGGCCTCCTTGAACATAGACAATGCTGGTAGTTTTATATGTACTTCCATTGCAAGGGGGGACGCAGATCTCCTTAAAAGGTTATTGGCAAATGGCATTGACCCGAATTCAAAAGATTATGATCACCGGACTCCACTTCATGTAGCTGCATCAGAAGGACTATACATAATGTCAAAGCTGCTGTTAGAAGCTGGGGCTAGCGTTTTCTCGAAGGACAGATGGGGAAATACCCCACTAGATGAAGGTCGGATGTGTGGGAACAAGAATTTAATCAAACTGCTGGAAGAAGCAAAGGCAGCTCAGTTGACAGAATTACCTTACTGTGCTCAAGAAATCACAGATAAAATGCACCCGAAGAAGTGCACGGTATACCCTTTCCACCCATGGGAAAGCAAAGAAGGAAGAAGACCTGGAATCGTGTTATGGGTCCCAAGTACCATTGGAGAGCTCATCAGAACAGCAGGAGAGCAACTGGAGTTTTCATCGAGTGGTTCCGGTATAATATTAACAGACGATGCAGGTAAAATTCTTGATGTGGACTTGATAAATGATGGTCAGAAGCTGTATTTGGTCAGTGAGACACATTTTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

836

Amino Acids

95.47

Weight (kDa)

6.15

Isoelectric Point (pI)

41.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	87 - 336	1.4e-25	Ion transport protein
Ion_trans_2	PF07885	276 - 332	5.2e-08	Ion channel
cNMP_binding	PF00027	426 - 511	2.5e-17	Cyclic nucleotide-binding domain
Ank_4	PF13637	557 - 603	8.4e-09	Ankyrin repeats (many copies)
Ank_2	PF12796	557 - 645	2e-15	Ankyrin repeats (3 copies)
Ank_5	PF13857	569 - 620	3.3e-09	Ankyrin repeats (many copies)
Ank	PF00023	582 - 614	6.6e-08	Ankyrin repeat
Ank_4	PF13637	590 - 634	1.4e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	656 - 733	2.8e-09	Ankyrin repeats (3 copies)
Ank_4	PF13637	657 - 700	1.5e-06	Ankyrin repeats (many copies)
Ank_5	PF13857	666 - 718	9.7e-09	Ankyrin repeats (many copies)
KHA	PF11834	757 - 828	4.3e-20	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	668
Acc16I	TGCGCA	1 cut(s)	599
Acc36I	ACCTGC	1 cut(s)	2423
AccI	GTMKAC	4 cut(s)	393, 1489, 1821, 2283
AccII	CGCG	3 cut(s)	51, 109, 111
AciI	CCGC	8 cut(s)	49, 111, 172, 175, 178, 707, 1552, 1670
AclWI	GGATC	6 cut(s)	90, 1009, 1083, 1332, 1702, 1718
AcoI	YGGCCR	3 cut(s)	25, 729, 870
AcsI	RAATTY	7 cut(s)	272, 1152, 1241, 1277, 2020, 2179, 2439
AcuI	CTGAAG	1 cut(s)	793
AcyI	GRCGYC	1 cut(s)	207
AfaI	GTAC	7 cut(s)	71, 296, 479, 761, 1123, 1957, 2350
AfeI	AGCGCT	1 cut(s)	833
AfiI	CCNNNNNNNGG	9 cut(s)	37, 55, 631, 1294, 1576, 1810, 1915, 1968, 2299
AgeI	ACCGGT	1 cut(s)	249
AjnI	CCWGG	5 cut(s)	659, 1146, 1288, 1309, 2323
AjuI	GAANNNNNNNTTGG	2 cut(s)	2465, 2497
Alw21I	GWGCWC	3 cut(s)	2242, 2279, 2364
Alw26I	GTCTC	4 cut(s)	29, 343, 420, 2486
Alw44I	GTGCAC	1 cut(s)	2275
AlwI	GGATC	6 cut(s)	90, 1009, 1083, 1332, 1702, 1718
AlwNI	CAGNNNCTG	3 cut(s)	85, 196, 2476
Aor51HI	AGCGCT	1 cut(s)	833
AoxI	GGCC	5 cut(s)	25, 708, 729, 870, 1917
ApaLI	GTGCAC	1 cut(s)	2275
ApeKI	GCWGC	5 cut(s)	1136, 1769, 2060, 2092, 2210
ApoI	RAATTY	7 cut(s)	272, 1152, 1241, 1277, 2020, 2179, 2439
ArsI	GACNNNNNNTTYG	4 cut(s)	216, 248, 1081, 1113
AsiGI	ACCGGT	1 cut(s)	249
Asp700I	GAANNNNTTC	2 cut(s)	1212, 1281
AspLEI	GCGC	4 cut(s)	111, 459, 600, 834
AspS9I	GGNCC	2 cut(s)	1917, 2341
AsuHPI	GGTGA	4 cut(s)	800, 1309, 1746, 2030
AsuNHI	GCTAGC	1 cut(s)	2111
AvaII	GGWCC	1 cut(s)	2341
BaeGI	GKGCMC	1 cut(s)	2279
BalI	TGGCCA	3 cut(s)	27, 731, 872
BanII	GRGCYC	2 cut(s)	219, 2364
BarI	GAAGNNNNNNTAC	2 cut(s)	287, 319
BbsI	GAAGAC	2 cut(s)	153, 2326
Bbv12I	GWGCWC	3 cut(s)	2242, 2279, 2364
BbvI	GCAGC	5 cut(s)	1123, 1756, 2047, 2079, 2222
BccI	CCATC	6 cut(s)	17, 1373, 1739, 1877, 2127, 2459
BceAI	ACGGC	1 cut(s)	220
BcgI	CGANNNNNNTGC	2 cut(s)	279, 313
BciT130I	CCWGG	5 cut(s)	661, 1148, 1290, 1311, 2325
BclI	TGATCA	2 cut(s)	1299, 2035
BcoDI	GTCTC	4 cut(s)	29, 343, 420, 2486
BfaI	CTAG	5 cut(s)	609, 626, 1604, 2112, 2150
BfmI	CTRYAG	1 cut(s)	793
BfoI	RGCGCY	1 cut(s)	835
BfuAI	ACCTGC	1 cut(s)	2423
BglII	AGATCT	3 cut(s)	1036, 1684, 1981
BisI	GCNGC	9 cut(s)	173, 176, 708, 1137, 1671, 1770, 2061, 2093, 2211
BlsI	GCNGC	9 cut(s)	174, 177, 709, 1138, 1672, 1771, 2062, 2094, 2212
Bme1390I	CCNGG	5 cut(s)	661, 1148, 1290, 1311, 2325
Bme18I	GGWCC	1 cut(s)	2341
BmgT120I	GGNCC	2 cut(s)	1917, 2341
BmiI	GGNNCC	7 cut(s)	116, 1308, 1410, 1918, 2342, 2343, 2407
BmrFI	CCNGG	5 cut(s)	661, 1148, 1290, 1311, 2325
BmsI	GCATC	5 cut(s)	1220, 1533, 1771, 2072, 2419
BmtI	GCTAGC	1 cut(s)	2115
BpiI	GAAGAC	2 cut(s)	153, 2326
BpmI	CTGGAG	5 cut(s)	1130, 1311, 1376, 1737, 2408
BpuEI	CTTGAG	2 cut(s)	1418, 2226
BsaBI	GATNNNNATC	1 cut(s)	2034
BsaHI	GRCGYC	1 cut(s)	207
BsaJI	CCNNGG	5 cut(s)	521, 867, 1288, 1678, 2297
BsaWI	WCCGGW	4 cut(s)	249, 462, 2040, 2408
BsaXI	ACNNNNNCTCC	2 cut(s)	53, 83
Bsc4I	CCNNNNNNNGG	9 cut(s)	37, 55, 631, 1294, 1576, 1810, 1915, 1968, 2299
Bse118I	RCCGGY	1 cut(s)	249
Bse1I	ACTGG	4 cut(s)	118, 366, 1720, 2391
Bse3DI	GCAATG	1 cut(s)	1962
Bse8I	GATNNNNATC	1 cut(s)	2034
BseBI	CCWGG	5 cut(s)	661, 1148, 1290, 1311, 2325
BseDI	CCNNGG	5 cut(s)	521, 867, 1288, 1678, 2297
BseGI	GGATG	7 cut(s)	28, 238, 273, 370, 1235, 1524, 2169
BseJI	GATNNNNATC	1 cut(s)	2034
BseLI	CCNNNNNNNGG	9 cut(s)	37, 55, 631, 1294, 1576, 1810, 1915, 1968, 2299
BseMI	GCAATG	1 cut(s)	1962
BseMII	CTCAG	4 cut(s)	77, 899, 1635, 2228
BseNI	ACTGG	4 cut(s)	118, 366, 1720, 2391
BseRI	GAGGAG	5 cut(s)	22, 71, 74, 565, 1373
BseSI	GKGCMC	1 cut(s)	2279
BseXI	GCAGC	5 cut(s)	1123, 1756, 2047, 2079, 2222
BseYI	CCCAGC	1 cut(s)	2105
BsgI	GTGCAG	2 cut(s)	98, 679
Bsh1236I	CGCG	3 cut(s)	51, 109, 111
Bsh1285I	CGRYCG	1 cut(s)	49
BshFI	GGCC	5 cut(s)	27, 710, 731, 872, 1919
BshTI	ACCGGT	1 cut(s)	249
BsiEI	CGRYCG	1 cut(s)	49
BsiHKAI	GWGCWC	3 cut(s)	2242, 2279, 2364
BsiSI	CCGG	4 cut(s)	250, 463, 2041, 2409
BslFI	GGGAC	4 cut(s)	827, 1037, 1988, 2327
BslI	CCNNNNNNNGG	9 cut(s)	37, 55, 631, 1294, 1576, 1810, 1915, 1968, 2299
BsmAI	GTCTC	4 cut(s)	29, 343, 420, 2486
BsmBI	CGTCTC	1 cut(s)	29
BsmFI	GGGAC	4 cut(s)	827, 1037, 1988, 2327
BsmI	GAATGC	1 cut(s)	383
BsnI	GGCC	5 cut(s)	27, 710, 731, 872, 1919
Bsp1286I	GDGCHC	4 cut(s)	219, 2242, 2279, 2364
Bsp19I	CCATGG	2 cut(s)	867, 2297
BspACI	CCGC	8 cut(s)	49, 111, 172, 175, 178, 707, 1552, 1670
BspANI	GGCC	5 cut(s)	27, 710, 731, 872, 1919
BspCNI	CTCAG	4 cut(s)	78, 900, 1636, 2227
BspFNI	CGCG	3 cut(s)	51, 109, 111
BspHI	TCATGA	1 cut(s)	619
BspLI	GGNNCC	7 cut(s)	116, 1308, 1410, 1918, 2342, 2343, 2407
BspMI	ACCTGC	1 cut(s)	2423
BspOI	GCTAGC	1 cut(s)	2115
BspPI	GGATC	6 cut(s)	90, 1009, 1083, 1332, 1702, 1718
BsrDI	GCAATG	1 cut(s)	1962
BsrFI	RCCGGY	1 cut(s)	249
BsrI	ACTGG	4 cut(s)	118, 366, 1720, 2391
BssAI	RCCGGY	1 cut(s)	249
BssECI	CCNNGG	5 cut(s)	521, 867, 1288, 1678, 2297
BssNAI	GTATAC	1 cut(s)	2284
BssNI	GRCGYC	1 cut(s)	207
BssT1I	CCWWGG	3 cut(s)	521, 867, 2297
Bst1107I	GTATAC	1 cut(s)	2284
Bst2UI	CCWGG	5 cut(s)	661, 1148, 1290, 1311, 2325
Bst4CI	ACNGT	7 cut(s)	32, 431, 477, 877, 1468, 2237, 2281
Bst6I	CTCTTC	2 cut(s)	60, 1226
BstACI	GRCGYC	1 cut(s)	207
BstC8I	GCNNGC	3 cut(s)	712, 744, 2113
BstDEI	CTNAG	5 cut(s)	86, 908, 1173, 1644, 2214
BstDSI	CCRYGG	3 cut(s)	867, 1678, 2297
BstEII	GGTNACC	1 cut(s)	1752
BstENI	CCTNNNNNAGG	3 cut(s)	629, 1574, 1808
BstF5I	GGATG	7 cut(s)	28, 238, 273, 370, 1235, 1524, 2169
BstFNI	CGCG	3 cut(s)	51, 109, 111
BstH2I	RGCGCY	1 cut(s)	835
BstHHI	GCGC	4 cut(s)	111, 459, 600, 834
BstMAI	GTCTC	4 cut(s)	29, 343, 420, 2486
BstMCI	CGRYCG	1 cut(s)	49
BstMWI	GCNNNNNNNGC	2 cut(s)	1649, 2210
BstNI	CCWGG	5 cut(s)	661, 1148, 1290, 1311, 2325
BstPI	GGTNACC	1 cut(s)	1752
BstSCI	CCNGG	5 cut(s)	659, 1146, 1288, 1309, 2323
BstSFI	CTRYAG	1 cut(s)	793
BstSLI	GKGCMC	1 cut(s)	2279
BstUI	CGCG	3 cut(s)	51, 109, 111
BstV1I	GCAGC	5 cut(s)	1123, 1756, 2047, 2079, 2222
BstV2I	GAAGAC	2 cut(s)	153, 2326
BstX2I	RGATCY	5 cut(s)	82, 1036, 1075, 1684, 1981
BstYI	RGATCY	5 cut(s)	82, 1036, 1075, 1684, 1981
BstZ17I	GTATAC	1 cut(s)	2284
BsuRI	GGCC	5 cut(s)	27, 710, 731, 872, 1919
BtgI	CCRYGG	3 cut(s)	867, 1678, 2297
BtgZI	GCGATG	1 cut(s)	400
BtsCI	GGATG	7 cut(s)	28, 238, 273, 370, 1235, 1524, 2169
BtsI	GCAGTG	1 cut(s)	297
BtsIMutI	CAGTG	3 cut(s)	297, 873, 2494
BveI	ACCTGC	1 cut(s)	2423
Cac8I	GCNNGC	3 cut(s)	712, 744, 2113
CaiI	CAGNNNCTG	3 cut(s)	85, 196, 2476
CciI	TCATGA	1 cut(s)	619
CfoI	GCGC	4 cut(s)	111, 459, 600, 834
Cfr10I	RCCGGY	1 cut(s)	249
Cfr13I	GGNCC	2 cut(s)	1917, 2341
CseI	GACGC	2 cut(s)	196, 1985
Csp6I	GTAC	7 cut(s)	70, 295, 478, 760, 1122, 1956, 2349
CspAI	ACCGGT	1 cut(s)	249
CspCI	CAANNNNNGTGG	2 cut(s)	526, 561
CviQI	GTAC	7 cut(s)	70, 295, 478, 760, 1122, 1956, 2349
DdeI	CTNAG	5 cut(s)	86, 908, 1173, 1644, 2214
EaeI	YGGCCR	3 cut(s)	25, 729, 870
Eam1104I	CTCTTC	2 cut(s)	60, 1226
EarI	CTCTTC	2 cut(s)	60, 1226
EciI	GGCGGA	1 cut(s)	1567
Ecl136II	GAGCTC	1 cut(s)	2362
Eco130I	CCWWGG	3 cut(s)	521, 867, 2297
Eco24I	GRGCYC	2 cut(s)	219, 2364
Eco47I	GGWCC	1 cut(s)	2341
Eco47III	AGCGCT	1 cut(s)	833
Eco53kI	GAGCTC	1 cut(s)	2362
Eco57I	CTGAAG	1 cut(s)	793
Eco91I	GGTNACC	1 cut(s)	1752
EcoICRI	GAGCTC	1 cut(s)	2362
EcoNI	CCTNNNNNAGG	3 cut(s)	629, 1574, 1808
EcoO109I	RGGNCCY	2 cut(s)	1917, 2341
EcoO65I	GGTNACC	1 cut(s)	1752
EcoRI	GAATTC	2 cut(s)	1241, 2020
EcoRII	CCWGG	5 cut(s)	659, 1146, 1288, 1309, 2323
EcoT14I	CCWWGG	3 cut(s)	521, 867, 2297
EcoT38I	GRGCYC	2 cut(s)	219, 2364
ErhI	CCWWGG	3 cut(s)	521, 867, 2297
Esp3I	CGTCTC	1 cut(s)	29
FalI	AAGNNNNNCTT	2 cut(s)	1380, 1412
FaqI	GGGAC	4 cut(s)	827, 1037, 1988, 2327
FauI	CCCGC	1 cut(s)	104
FbaI	TGATCA	2 cut(s)	1299, 2035
FblI	GTMKAC	4 cut(s)	393, 1489, 1821, 2283
Fnu4HI	GCNGC	9 cut(s)	173, 176, 708, 1137, 1671, 1770, 2061, 2093, 2211
FokI	GGATG	7 cut(s)	35, 225, 280, 377, 1242, 1511, 2176
FriOI	GRGCYC	2 cut(s)	219, 2364
Fsp4HI	GCNGC	9 cut(s)	173, 176, 708, 1137, 1671, 1770, 2061, 2093, 2211
FspBI	CTAG	5 cut(s)	609, 626, 1604, 2112, 2150
FspI	TGCGCA	1 cut(s)	599
GlaI	GCGC	4 cut(s)	110, 458, 599, 833
GluI	GCNGC	9 cut(s)	173, 176, 708, 1137, 1671, 1770, 2061, 2093, 2211
GsaI	CCCAGC	1 cut(s)	2109
GsuI	CTGGAG	5 cut(s)	1130, 1311, 1376, 1737, 2408
HaeII	RGCGCY	1 cut(s)	835
HaeIII	GGCC	5 cut(s)	27, 710, 731, 872, 1919
HapII	CCGG	4 cut(s)	250, 463, 2041, 2409
HgaI	GACGC	2 cut(s)	196, 1985
HhaI	GCGC	4 cut(s)	111, 459, 600, 834
Hin1I	GRCGYC	1 cut(s)	207
Hin6I	GCGC	4 cut(s)	109, 457, 598, 832
HinP1I	GCGC	4 cut(s)	109, 457, 598, 832
HincII	GTYRAC	2 cut(s)	502, 2220
HindII	GTYRAC	2 cut(s)	502, 2220
HindIII	AAGCTT	2 cut(s)	671, 805
HinfI	GANTC	8 cut(s)	96, 147, 228, 351, 952, 1607, 2044, 2328
HpaII	CCGG	4 cut(s)	250, 463, 2041, 2409
HphI	GGTGA	4 cut(s)	800, 1309, 1746, 2030
Hpy99I	CGWCG	5 cut(s)	56, 109, 158, 206, 1322
HpyCH4III	ACNGT	7 cut(s)	32, 431, 477, 877, 1468, 2237, 2281
HpyCH4IV	ACGT	1 cut(s)	1317
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1649, 2210
HpyF3I	CTNAG	5 cut(s)	86, 908, 1173, 1644, 2214
HpySE526I	ACGT	1 cut(s)	1317
Hsp92I	GRCGYC	1 cut(s)	207
HspAI	GCGC	4 cut(s)	109, 457, 598, 832
KflI	GGGWCCC	1 cut(s)	2341
Ksp22I	TGATCA	2 cut(s)	1299, 2035
LmnI	GCTCC	3 cut(s)	114, 959, 1718
Lsp1109I	GCAGC	5 cut(s)	1123, 1756, 2047, 2079, 2222
LweI	GCATC	5 cut(s)	1220, 1533, 1771, 2072, 2419
MaeI	CTAG	5 cut(s)	609, 626, 1604, 2112, 2150
MaeII	ACGT	1 cut(s)	1317
MaeIII	GTNAC	7 cut(s)	431, 604, 788, 862, 974, 1097, 1752
MflI	RGATCY	5 cut(s)	82, 1036, 1075, 1684, 1981
MhlI	GDGCHC	4 cut(s)	219, 2242, 2279, 2364
MlsI	TGGCCA	3 cut(s)	27, 731, 872
MluNI	TGGCCA	3 cut(s)	27, 731, 872
MlyI	GAGTC	5 cut(s)	141, 222, 345, 1616, 2038
MmeI	TCCRAC	2 cut(s)	1933, 2141
Mox20I	TGGCCA	3 cut(s)	27, 731, 872
MroXI	GAANNNNTTC	2 cut(s)	1212, 1281
MscI	TGGCCA	3 cut(s)	27, 731, 872
MseI	TTAA	6 cut(s)	989, 1263, 1775, 1989, 2183, 2420
Msp20I	TGGCCA	3 cut(s)	27, 731, 872
MspA1I	CMGCKG	1 cut(s)	707
MspI	CCGG	4 cut(s)	250, 463, 2041, 2409
MspR9I	CCNGG	5 cut(s)	661, 1148, 1290, 1311, 2325
Mva1269I	GAATGC	1 cut(s)	383
MvaI	CCWGG	5 cut(s)	661, 1148, 1290, 1311, 2325
MvnI	CGCG	3 cut(s)	51, 109, 111
MwoI	GCNNNNNNNGC	2 cut(s)	1649, 2210
NcoI	CCATGG	2 cut(s)	867, 2297
NheI	GCTAGC	1 cut(s)	2111
NlaIV	GGNNCC	7 cut(s)	116, 1308, 1410, 1918, 2342, 2343, 2407
NmeAIII	GCCGAG	1 cut(s)	129
NmuCI	GTSAC	5 cut(s)	431, 604, 788, 1097, 1752
NsbI	TGCGCA	1 cut(s)	599
PagI	TCATGA	1 cut(s)	619
PcsI	WCGNNNNNNNCGW	1 cut(s)	51
PctI	GAATGC	1 cut(s)	383
PdmI	GAANNNNTTC	2 cut(s)	1212, 1281
PfeI	GAWTC	3 cut(s)	96, 952, 2328
PflFI	GACNNNGTC	1 cut(s)	206
PfoI	TCCNGGA	1 cut(s)	1146
PinAI	ACCGGT	1 cut(s)	249
PkrI	GCNGC	9 cut(s)	174, 177, 709, 1138, 1672, 1771, 2062, 2094, 2212
PleI	GAGTC	5 cut(s)	141, 222, 345, 1615, 2038
PpsI	GAGTC	5 cut(s)	141, 222, 345, 1615, 2038
PpuMI	RGGWCCY	1 cut(s)	2341
PsiI	TTATAA	1 cut(s)	668
Psp124BI	GAGCTC	1 cut(s)	2364
Psp5II	RGGWCCY	1 cut(s)	2341
Psp6I	CCWGG	5 cut(s)	659, 1146, 1288, 1309, 2323
PspEI	GGTNACC	1 cut(s)	1752
PspFI	CCCAGC	1 cut(s)	2105
PspGI	CCWGG	5 cut(s)	659, 1146, 1288, 1309, 2323
PspN4I	GGNNCC	7 cut(s)	116, 1308, 1410, 1918, 2342, 2343, 2407
PspPI	GGNCC	2 cut(s)	1917, 2341
PspPPI	RGGWCCY	1 cut(s)	2341
PstNI	CAGNNNCTG	3 cut(s)	85, 196, 2476
PsuI	RGATCY	5 cut(s)	82, 1036, 1075, 1684, 1981
PsyI	GACNNNGTC	1 cut(s)	206
RsaI	GTAC	7 cut(s)	71, 296, 479, 761, 1123, 1957, 2350
RsaNI	GTAC	7 cut(s)	70, 295, 478, 760, 1122, 1956, 2349
SacI	GAGCTC	1 cut(s)	2364
SaqAI	TTAA	6 cut(s)	989, 1263, 1775, 1989, 2183, 2420
SatI	GCNGC	9 cut(s)	173, 176, 708, 1137, 1671, 1770, 2061, 2093, 2211
Sau96I	GGNCC	2 cut(s)	1917, 2341
SchI	GAGTC	5 cut(s)	141, 222, 345, 1616, 2038
ScrFI	CCNGG	5 cut(s)	661, 1148, 1290, 1311, 2325
SduI	GDGCHC	4 cut(s)	219, 2242, 2279, 2364
SfaNI	GCATC	5 cut(s)	1220, 1533, 1771, 2072, 2419
SfcI	CTRYAG	1 cut(s)	793
SinI	GGWCC	1 cut(s)	2341
SmlI	CTYRAG	2 cut(s)	1397, 2241
SmoI	CTYRAG	2 cut(s)	1397, 2241
SsiI	CCGC	8 cut(s)	49, 111, 172, 175, 178, 707, 1552, 1670
SspI	AATATT	1 cut(s)	2418
SspMI	CTAG	5 cut(s)	609, 626, 1604, 2112, 2150
SstI	GAGCTC	1 cut(s)	2364
StyD4I	CCNGG	5 cut(s)	659, 1146, 1288, 1309, 2323
StyI	CCWWGG	3 cut(s)	521, 867, 2297
TaaI	ACNGT	7 cut(s)	32, 431, 477, 877, 1468, 2237, 2281
TaiI	ACGT	1 cut(s)	1320
TatI	WGTACW	2 cut(s)	294, 1955
TauI	GCSGC	4 cut(s)	175, 178, 710, 1673
TfiI	GAWTC	3 cut(s)	96, 952, 2328
Tru1I	TTAA	6 cut(s)	989, 1263, 1775, 1989, 2183, 2420
Tru9I	TTAA	6 cut(s)	989, 1263, 1775, 1989, 2183, 2420
TscAI	CASTG	3 cut(s)	297, 880, 2494
TseFI	GTSAC	5 cut(s)	431, 604, 788, 1097, 1752
TseI	GCWGC	5 cut(s)	1136, 1769, 2060, 2092, 2210
Tsp45I	GTSAC	5 cut(s)	431, 604, 788, 1097, 1752
TspGWI	ACGGA	4 cut(s)	53, 1070, 1145, 1695
TspRI	CASTG	3 cut(s)	297, 880, 2494
Tth111I	GACNNNGTC	1 cut(s)	206
VneI	GTGCAC	1 cut(s)	2275
VpaK11BI	GGWCC	1 cut(s)	2341
XagI	CCTNNNNNAGG	3 cut(s)	629, 1574, 1808
XapI	RAATTY	7 cut(s)	272, 1152, 1241, 1277, 2020, 2179, 2439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	865
XmiI	GTMKAC	4 cut(s)	393, 1489, 1821, 2283
XmnI	GAANNNNTTC	2 cut(s)	1212, 1281
XspI	CTAG	5 cut(s)	609, 626, 1604, 2112, 2150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.