Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Forward (+)

66837122 .. 66844792

Gene Structure 7671 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00275370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGCATGGTGCTCAAGAAAGACGAAGATCGTCAGGAGGAGCTGAGGGTCACCGCCACGACTCCTCTGACGATGAAGGATTCGAGGTCGAGGACGACGAGCTCATCACCGATTCGAGGTCGTCGTCGAGACAGTTCTGGTGGAAAAGGCGTTTCTCTTTGAAGAGAAGTGAGTCTGCAGGCACTAATGCCACCACTGTTGAGACTACTGCGGCCAATGCAAGCAGAACATCATCATCATCGGCTTCTAATTCGCTTTTCAGTAGAATGGTCATTCATCCCGATAACTGGTGGTACGTGGGGTGGACGCATTTCATTCTGTTATGGGCAGTCTACTCCTCCTTCTTCACACCTCTGGAGTTCGGCTTCTTCAGAGGCCTGCCGGAGAACCTGTTCATGCTCGACAGCTGCGGCCAGATAGCCTTTCTCATCGATATCATCGTCCGCTTCTTCGTCGCCTATCGCGACTTCCACTCCCACCGCATTGTCTACGATCGCCACCTCATCGCTCTCCGATACCTCAAGTCTCGTTTCCTCGTCGATTTTCTCGGCTGTCTTCCCTGGGATGCCATCTATAAGGTTAATGGCATGTGTAATGCATGGAGTCATGGAGAGAATGATGGAAATGAAACATCAAGTACATTGTATGGAAGTGTGAATGCGGACGTCGACGCGACGGCGCTTGCGGGCGGCGACGGAGGCGTGGGGCTTGCAGGGAGGGAGGGCGGGAGGCGTCCGGACCGTTTGAGGTCGGCCTTGCGGTTGGCGCGCCACCTCACCTGCTGCTGCGGGTTCGTGCAAGATCTCGCAAAACCCGTCGCCAACGACTCCACCCGACCGCGACCCCTCCGCACGACCCCCGGCCTCCCTCGGGCAAGCCGCGCCGCGCCGTCGCCGCCGGATCGAGGCCGGAGCGATGACGCTTCTGGGAGAGAAGAGGCGGTGAGGTACTTATTGTGGATACGGCTAAGCCGGGCACGGAGAGTAACGGAGTTTTTTGAGAAATTGGAGAAAGATATCAGAATCAATTACCTGTTTACAAGAATTGCCAAGCTATTTGTTGTTGAACTTTATAGCACACACACAGCTGCTTGCATATTTTATTATCTGGCTACTACCATGCCTCCATCACAGGAAGGTTACACATGGATTGGGAGTTTGAAGATGGGTGACTATAGCTATGCACATTTCAGGGAGATTGATCTTTGGAAGCGTTACATAACTTCGCTCTATTTTGCAGTTGTTACCATGGCCACCGTTGGTTATGGAGAGATACATGCAGTGAATGTGAGGGAGATGATATTCATCATGTTTTATGTGTCTTTTGATATGATTCTTGGTGCTTATCTGCTTGGTAACATTGCGGCATTGATAGTGAAAGGATCAAAAACAGAGAAGTTTAGGGACAAGATGGCGGACCTTATCAAATACATGAATAGAAACCGTATTGATAGAGGGATAAGTAAGGAAATTAAAAACCATTTGAGATTACAGTATGACCGTAGCTACACCGAAGCTGCTGTTCTTCAGGATATCCCTGCCTCTATTCGCAGTAAGATTTCACAGAAATTGTATGAGCCATACATCAAAGAAGTTCATCTTTTTAAAGGGTGCTCTGCAGGATTCATAAAACAGATAGAAAAGCTGGGAAGGGGTGAAGATGATGACAGTGAAGAGCTGCTTGATCGTTTGCAAACCTATAGCTCATTTGGAGAGGTTTCTTTTCTTTGCAACACTCCCCAGCCGTATGCTGTTCGGGTTTGTCAACTGTGTAGGGTCTTACGACTTGATAGACAGTCCTTTAGAGAAATACTGGAGATATATTTCTTAGATGGCCGGCTCATCTTGGATAACCTGCTTGAGGGAAAAGACTTGTATACCCGGAATAAGCTTTTGGAATCAGATATCACTCTATATATCGGAAAGCATGAAATGGAGTTAGTTATGAAAGTAAATTGTGCTGTCTATGATGGAGATTTTTATCTAGTAAAACGTTTGATTGGAGCTGGTGTAGATCCAAACAAGGTGGATTATGCTGGAAGGTCCCCTTTGCATGTTGCTGCATCAAAAGGGTACGAGGATATTGCTCGTTTTCTTATTGAAAAAGGGGAGACGTCAAGATGTCAGCTTTCCGTATTACGTGCTGTACCAGATAAGTTTGGGAAGACTCCATTGCTTGAAGCCATAAAAAATGGGCATGATCAAGTTGCTTCTTTACTTGTTCAGGCAGGGGCATCACTTACTATTGAAGATGCTGGTGATTTTCTCTGTGCTACTGTAGCAAAGAGGGATTTGGACCTCGTAAAAAGGCTTTTAGCCAATGACATGAATCCAAATGCGAAAAATTATGATCAGAGATCACCACTACATGTAGCTGCTTCAGAAGGGTTATATCCAATGGCAGAGTTCCTATTAGAAGCAGGAGCAAGTGTTCTATCAAAGGACAGATGGGGGAAAACTCCACTTGATGAAGCCCGCATAGGTGGAAGTAACAAGCTAATGCAATTGCTCGAATCTACAAGACTTTCTCAGTTGTCAGAGTTCTCTGATGGTTCTCAAGTAGTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

855

Amino Acids

96.68

Weight (kDa)

8.05

Isoelectric Point (pI)

43.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	101 - 193	5.8e-06	Ion transport protein
Ion_trans	PF00520	314 - 462	2.7e-16	Ion transport protein
Ion_trans_2	PF07885	402 - 458	2.2e-08	Ion channel
cNMP_binding	PF00027	553 - 605	1.7e-07	Cyclic nucleotide-binding domain
Ank_4	PF13637	652 - 699	2.7e-10	Ankyrin repeats (many copies)
Ank_2	PF12796	653 - 750	9.1e-16	Ankyrin repeats (3 copies)
Ank_2	PF12796	759 - 836	3.2e-08	Ankyrin repeats (3 copies)
Ank_4	PF13637	760 - 804	3e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	770 - 824	5.3e-10	Ankyrin repeats (many copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	785
AatII	GACGTC	2 cut(s)	666, 2114
Acc36I	ACCTGC	2 cut(s)	785, 1860
AccI	GTMKAC	4 cut(s)	330, 486, 666, 1874
AccII	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
AccIII	TCCGGA	1 cut(s)	733
AclI	AACGTT	1 cut(s)	1990
AclWI	GGATC	3 cut(s)	906, 1387, 2006
AcoI	YGGCCR	4 cut(s)	210, 409, 1248, 1831
AcuI	CTGAAG	3 cut(s)	352, 1508, 2361
AcyI	GRCGYC	3 cut(s)	663, 730, 2111
AfaI	GTAC	5 cut(s)	293, 637, 947, 2072, 2145
AfiI	CCNNNNNNNGG	5 cut(s)	114, 285, 868, 902, 1858
AflIII	ACRYGT	1 cut(s)	2365
AgsI	TTSAA	6 cut(s)	160, 1064, 1159, 2099, 2177, 2246
AjnI	CCWGG	1 cut(s)	557
AjuI	GAANNNNNNNTTGG	2 cut(s)	1874, 1906
AloI	GAACNNNNNNTCC	4 cut(s)	374, 406, 2412, 2444
Alw21I	GWGCWC	3 cut(s)	13, 102, 1613
Alw26I	GTCTC	4 cut(s)	121, 194, 528, 2102
AlwI	GGATC	3 cut(s)	906, 1387, 2006
Ama87I	CYCGRG	1 cut(s)	867
Aor13HI	TCCGGA	1 cut(s)	733
AoxI	GGCC	8 cut(s)	210, 373, 409, 750, 859, 904, 1248, 1831
ApeKI	GCWGC	8 cut(s)	405, 780, 783, 1085, 1514, 1675, 2057, 2372
AscI	GGCGCGCC	1 cut(s)	764
Asp700I	GAANNNNTTC	1 cut(s)	389
AspLEI	GCGC	5 cut(s)	679, 766, 768, 881, 886
AspS9I	GGNCC	4 cut(s)	736, 1414, 2040, 2293
AsuC2I	CCSGG	3 cut(s)	858, 971, 1879
AsuHPI	GGTGA	8 cut(s)	41, 97, 766, 952, 1178, 1664, 2267, 2349
AvaI	CYCGRG	1 cut(s)	867
AvaII	GGWCC	4 cut(s)	736, 1414, 2040, 2293
BaeGI	GKGCMC	1 cut(s)	976
BalI	TGGCCA	1 cut(s)	1250
BanII	GRGCYC	1 cut(s)	102
BbsI	GAAGAC	2 cut(s)	545, 2168
Bbv12I	GWGCWC	3 cut(s)	13, 102, 1613
BbvCI	CCTCAGC	1 cut(s)	42
BbvI	GCAGC	8 cut(s)	392, 767, 770, 1072, 1501, 1662, 2044, 2359
BccI	CCATC	9 cut(s)	575, 611, 1132, 1156, 1402, 1823, 1961, 2439, 2540
BceAI	ACGGC	4 cut(s)	690, 871, 977, 1726
BcgI	CGANNNNNNTGC	2 cut(s)	2063, 2097
BciT130I	CCWGG	1 cut(s)	559
BciVI	GTATCC	1 cut(s)	951
BclI	TGATCA	2 cut(s)	2197, 2347
BcnI	CCSGG	3 cut(s)	858, 971, 1879
BcoDI	GTCTC	4 cut(s)	121, 194, 528, 2102
BfaI	CTAG	1 cut(s)	1982
BfmI	CTRYAG	5 cut(s)	174, 1171, 1614, 1696, 2274
BfoI	RGCGCY	1 cut(s)	680
BfuAI	ACCTGC	2 cut(s)	785, 1860
BfuI	GTATCC	1 cut(s)	951
BglII	AGATCT	1 cut(s)	799
BlpI	GCTNAGC	1 cut(s)	965
Bme1390I	CCNGG	4 cut(s)	559, 858, 971, 1879
Bme18I	GGWCC	4 cut(s)	736, 1414, 2040, 2293
BmeT110I	CYCGRG	1 cut(s)	867
BmgT120I	GGNCC	4 cut(s)	736, 1414, 2040, 2293
BmiI	GGNNCC	1 cut(s)	2042
BmrFI	CCNGG	4 cut(s)	559, 858, 971, 1879
BmsI	GCATC	4 cut(s)	553, 2069, 2239, 2240
BpiI	GAAGAC	2 cut(s)	545, 2168
BpmI	CTGGAG	2 cut(s)	374, 1832
Bpu10I	CCTNAGC	1 cut(s)	42
Bpu1102I	GCTNAGC	1 cut(s)	965
BpuEI	CTTGAG	3 cut(s)	503, 1877, 2538
BpuMI	CCSGG	3 cut(s)	858, 971, 1879
Bsa29I	ATCGAT	1 cut(s)	429
BsaAI	YACGTR	2 cut(s)	295, 2138
BsaBI	GATNNNNATC	2 cut(s)	1301, 1977
BsaHI	GRCGYC	3 cut(s)	663, 730, 2111
BsaJI	CCNNGG	5 cut(s)	557, 558, 856, 866, 1245
BsaWI	WCCGGW	1 cut(s)	733
Bsc4I	CCNNNNNNNGG	5 cut(s)	114, 285, 868, 902, 1858
Bse118I	RCCGGY	1 cut(s)	1833
Bse1I	ACTGG	2 cut(s)	290, 1815
Bse3DI	GCAATG	2 cut(s)	1356, 2168
Bse8I	GATNNNNATC	2 cut(s)	1301, 1977
BseAI	TCCGGA	1 cut(s)	733
BseBI	CCWGG	1 cut(s)	559
BseCI	ATCGAT	1 cut(s)	429
BseDI	CCNNGG	5 cut(s)	557, 558, 856, 866, 1245
BseGI	GGATG	2 cut(s)	274, 568
BseJI	GATNNNNATC	2 cut(s)	1301, 1977
BseLI	CCNNNNNNNGG	5 cut(s)	114, 285, 868, 902, 1858
BseMI	GCAATG	2 cut(s)	1356, 2168
BseMII	CTCAG	2 cut(s)	33, 2540
BseNI	ACTGG	2 cut(s)	290, 1815
BsePI	GCGCGC	1 cut(s)	764
BseRI	GAGGAG	3 cut(s)	51, 52, 325
BseSI	GKGCMC	1 cut(s)	976
BseXI	GCAGC	8 cut(s)	392, 767, 770, 1072, 1501, 1662, 2044, 2359
BseYI	CCCAGC	2 cut(s)	1642, 1737
Bsh1236I	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
Bsh1285I	CGRYCG	2 cut(s)	493, 836
BshFI	GGCC	8 cut(s)	212, 375, 411, 752, 861, 906, 1250, 1833
BshVI	ATCGAT	1 cut(s)	429
BsiEI	CGRYCG	2 cut(s)	493, 836
BsiHKAI	GWGCWC	3 cut(s)	13, 102, 1613
BsiHKCI	CYCGRG	1 cut(s)	867
BsiSI	CCGG	8 cut(s)	380, 734, 858, 896, 907, 970, 1834, 1879
BslFI	GGGAC	2 cut(s)	1415, 2026
BslI	CCNNNNNNNGG	5 cut(s)	114, 285, 868, 902, 1858
BsmAI	GTCTC	4 cut(s)	121, 194, 528, 2102
BsmBI	CGTCTC	1 cut(s)	2102
BsmFI	GGGAC	2 cut(s)	1415, 2026
BsmI	GAATGC	1 cut(s)	661
BsnI	GGCC	8 cut(s)	212, 375, 411, 752, 861, 906, 1250, 1833
BsoBI	CYCGRG	1 cut(s)	867
Bsp1286I	GDGCHC	4 cut(s)	13, 102, 976, 1613
Bsp13I	TCCGGA	1 cut(s)	733
Bsp1720I	GCTNAGC	1 cut(s)	965
Bsp19I	CCATGG	1 cut(s)	1245
Bsp68I	TCGCGA	1 cut(s)	462
BspANI	GGCC	8 cut(s)	212, 375, 411, 752, 861, 906, 1250, 1833
BspCNI	CTCAG	2 cut(s)	34, 2539
BspDI	ATCGAT	1 cut(s)	429
BspEI	TCCGGA	1 cut(s)	733
BspFNI	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
BspLI	GGNNCC	1 cut(s)	2042
BspMAI	CTGCAG	2 cut(s)	178, 1618
BspMI	ACCTGC	2 cut(s)	785, 1860
BspPI	GGATC	3 cut(s)	906, 1387, 2006
BspQI	GCTCTTC	1 cut(s)	1665
BsrDI	GCAATG	2 cut(s)	1356, 2168
BsrFI	RCCGGY	1 cut(s)	1833
BsrI	ACTGG	2 cut(s)	290, 1815
BssAI	RCCGGY	1 cut(s)	1833
BssECI	CCNNGG	5 cut(s)	557, 558, 856, 866, 1245
BssHII	GCGCGC	1 cut(s)	764
BssNAI	GTATAC	1 cut(s)	1875
BssNI	GRCGYC	3 cut(s)	663, 730, 2111
BssT1I	CCWWGG	1 cut(s)	1245
Bst1107I	GTATAC	1 cut(s)	1875
Bst2UI	CCWGG	1 cut(s)	559
Bst6I	CTCTTC	3 cut(s)	155, 927, 1665
BstACI	GRCGYC	3 cut(s)	663, 730, 2111
BstBAI	YACGTR	2 cut(s)	295, 2138
BstDEI	CTNAG	4 cut(s)	42, 965, 1825, 2526
BstDSI	CCRYGG	1 cut(s)	1245
BstEII	GGTNACC	1 cut(s)	47
BstENI	CCTNNNNNAGG	1 cut(s)	1856
BstF5I	GGATG	2 cut(s)	274, 568
BstFNI	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
BstH2I	RGCGCY	1 cut(s)	680
BstHHI	GCGC	5 cut(s)	679, 766, 768, 881, 886
BstMAI	GTCTC	4 cut(s)	121, 194, 528, 2102
BstMCI	CGRYCG	2 cut(s)	493, 836
BstMWI	GCNNNNNNNGC	6 cut(s)	215, 696, 763, 878, 892, 2276
BstNI	CCWGG	1 cut(s)	559
BstNSI	RCATGY	4 cut(s)	589, 1277, 2054, 2369
BstPI	GGTNACC	1 cut(s)	47
BstSCI	CCNGG	4 cut(s)	557, 856, 969, 1877
BstSFI	CTRYAG	5 cut(s)	174, 1171, 1614, 1696, 2274
BstSLI	GKGCMC	1 cut(s)	976
BstUI	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
BstV1I	GCAGC	8 cut(s)	392, 767, 770, 1072, 1501, 1662, 2044, 2359
BstV2I	GAAGAC	2 cut(s)	545, 2168
BstX2I	RGATCY	2 cut(s)	799, 2011
BstYI	RGATCY	2 cut(s)	799, 2011
BstZ17I	GTATAC	1 cut(s)	1875
Bsu15I	ATCGAT	1 cut(s)	429
BsuI	GTATCC	1 cut(s)	951
BsuRI	GGCC	8 cut(s)	212, 375, 411, 752, 861, 906, 1250, 1833
BsuTUI	ATCGAT	1 cut(s)	429
BtgI	CCRYGG	1 cut(s)	1245
BtgZI	GCGATG	2 cut(s)	487, 927
BtsCI	GGATG	2 cut(s)	274, 568
BtsI	GCAGTG	1 cut(s)	1284
BtsIMutI	CAGTG	3 cut(s)	192, 1284, 1672
BtuMI	TCGCGA	1 cut(s)	462
BveI	ACCTGC	2 cut(s)	785, 1860
CfoI	GCGC	5 cut(s)	679, 766, 768, 881, 886
Cfr10I	RCCGGY	1 cut(s)	1833
Cfr13I	GGNCC	4 cut(s)	736, 1414, 2040, 2293
ClaI	ATCGAT	1 cut(s)	429
CpoI	CGGWCCG	1 cut(s)	736
CseI	GACGC	4 cut(s)	313, 677, 719, 926
Csp6I	GTAC	5 cut(s)	292, 636, 946, 2071, 2144
CspCI	CAANNNNNGTGG	4 cut(s)	178, 213, 2004, 2039
CspI	CGGWCCG	1 cut(s)	736
CviQI	GTAC	5 cut(s)	292, 636, 946, 2071, 2144
DdeI	CTNAG	4 cut(s)	42, 965, 1825, 2526
DraI	TTTAAA	1 cut(s)	1603
EaeI	YGGCCR	4 cut(s)	210, 409, 1248, 1831
Eam1104I	CTCTTC	3 cut(s)	155, 927, 1665
EarI	CTCTTC	3 cut(s)	155, 927, 1665
EciI	GGCGGA	1 cut(s)	1427
Ecl136II	GAGCTC	1 cut(s)	100
Eco130I	CCWWGG	1 cut(s)	1245
Eco147I	AGGCCT	1 cut(s)	375
Eco24I	GRGCYC	1 cut(s)	102
Eco32I	GATATC	4 cut(s)	433, 1015, 1531, 1903
Eco47I	GGWCC	4 cut(s)	736, 1414, 2040, 2293
Eco53kI	GAGCTC	1 cut(s)	100
Eco57I	CTGAAG	3 cut(s)	352, 1508, 2361
Eco88I	CYCGRG	1 cut(s)	867
Eco91I	GGTNACC	1 cut(s)	47
EcoICRI	GAGCTC	1 cut(s)	100
EcoNI	CCTNNNNNAGG	1 cut(s)	1856
EcoO109I	RGGNCCY	1 cut(s)	2040
EcoO65I	GGTNACC	1 cut(s)	47
EcoRII	CCWGG	1 cut(s)	557
EcoRV	GATATC	4 cut(s)	433, 1015, 1531, 1903
EcoT14I	CCWWGG	1 cut(s)	1245
EcoT22I	ATGCAT	2 cut(s)	6, 598
EcoT38I	GRGCYC	1 cut(s)	102
ErhI	CCWWGG	1 cut(s)	1245
Esp3I	CGTCTC	1 cut(s)	2102
FalI	AAGNNNNNCTT	6 cut(s)	1581, 1613, 1662, 1694, 2029, 2061
FaqI	GGGAC	2 cut(s)	1415, 2026
FauI	CCCGC	4 cut(s)	676, 716, 779, 2480
FbaI	TGATCA	2 cut(s)	2197, 2347
FblI	GTMKAC	4 cut(s)	330, 486, 666, 1874
FokI	GGATG	2 cut(s)	261, 575
FriOI	GRGCYC	1 cut(s)	102
FspBI	CTAG	1 cut(s)	1982
GlaI	GCGC	5 cut(s)	678, 765, 767, 880, 885
GsaI	CCCAGC	2 cut(s)	1646, 1741
GsuI	CTGGAG	2 cut(s)	374, 1832
HaeII	RGCGCY	1 cut(s)	680
HaeIII	GGCC	8 cut(s)	212, 375, 411, 752, 861, 906, 1250, 1833
HapII	CCGG	8 cut(s)	380, 734, 858, 896, 907, 970, 1834, 1879
HgaI	GACGC	4 cut(s)	313, 677, 719, 926
HhaI	GCGC	5 cut(s)	679, 766, 768, 881, 886
Hin1I	GRCGYC	3 cut(s)	663, 730, 2111
Hin6I	GCGC	5 cut(s)	677, 764, 766, 879, 884
HinP1I	GCGC	5 cut(s)	677, 764, 766, 879, 884
HincII	GTYRAC	2 cut(s)	667, 1763
HindII	GTYRAC	2 cut(s)	667, 1763
HindIII	AAGCTT	1 cut(s)	1886
HpaII	CCGG	8 cut(s)	380, 734, 858, 896, 907, 970, 1834, 1879
HphI	GGTGA	8 cut(s)	41, 97, 766, 952, 1178, 1664, 2267, 2349
Hpy166II	GTNNAC	7 cut(s)	303, 331, 487, 667, 1035, 1763, 1875
Hpy188III	TCNNGA	9 cut(s)	14, 33, 126, 278, 353, 461, 734, 1526, 2115
Hpy8I	GTNNAC	7 cut(s)	303, 331, 487, 667, 1035, 1763, 1875
HpyAV	CCTTC	6 cut(s)	68, 349, 1127, 1641, 2031, 2375
HpyCH4IV	ACGT	5 cut(s)	294, 663, 1990, 2111, 2137
HpyF10VI	GCNNNNNNNGC	6 cut(s)	215, 696, 763, 878, 892, 2276
HpyF3I	CTNAG	4 cut(s)	42, 965, 1825, 2526
HpySE526I	ACGT	5 cut(s)	294, 663, 1990, 2111, 2137
Hsp92I	GRCGYC	3 cut(s)	663, 730, 2111
HspAI	GCGC	5 cut(s)	677, 764, 766, 879, 884
Kpn2I	TCCGGA	1 cut(s)	733
KroI	GCCGGC	1 cut(s)	1833
KroNI	GCCGGC	1 cut(s)	1835
Ksp22I	TGATCA	2 cut(s)	2197, 2347
LguI	GCTCTTC	1 cut(s)	1665
LmnI	GCTCC	4 cut(s)	38, 909, 2000, 2420
Lsp1109I	GCAGC	8 cut(s)	392, 767, 770, 1072, 1501, 1662, 2044, 2359
LweI	GCATC	4 cut(s)	553, 2069, 2239, 2240
MaeI	CTAG	1 cut(s)	1982
MaeII	ACGT	5 cut(s)	294, 663, 1990, 2111, 2137
MaeIII	GTNAC	8 cut(s)	47, 982, 1136, 1166, 1211, 1240, 1352, 2486
MfeI	CAATTG	1 cut(s)	2501
MflI	RGATCY	2 cut(s)	799, 2011
MhlI	GDGCHC	4 cut(s)	13, 102, 976, 1613
MlsI	TGGCCA	1 cut(s)	1250
MluNI	TGGCCA	1 cut(s)	1250
MlyI	GAGTC	5 cut(s)	53, 179, 610, 818, 2158
Mox20I	TGGCCA	1 cut(s)	1250
Mph1103I	ATGCAT	2 cut(s)	6, 598
MroI	TCCGGA	1 cut(s)	733
MroNI	GCCGGC	1 cut(s)	1833
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	1250
MseI	TTAA	3 cut(s)	579, 1470, 1602
Msp20I	TGGCCA	1 cut(s)	1250
MspA1I	CMGCKG	2 cut(s)	405, 1085
MspI	CCGG	8 cut(s)	380, 734, 858, 896, 907, 970, 1834, 1879
MspR9I	CCNGG	4 cut(s)	559, 858, 971, 1879
MteI	GCGCNGCGC	1 cut(s)	882
MunI	CAATTG	1 cut(s)	2501
Mva1269I	GAATGC	1 cut(s)	661
MvaI	CCWGG	1 cut(s)	559
MvnI	CGCG	6 cut(s)	462, 671, 766, 838, 879, 884
MwoI	GCNNNNNNNGC	6 cut(s)	215, 696, 763, 878, 892, 2276
NaeI	GCCGGC	1 cut(s)	1835
NciI	CCSGG	3 cut(s)	858, 971, 1879
NcoI	CCATGG	1 cut(s)	1245
NgoMIV	GCCGGC	1 cut(s)	1833
NlaIV	GGNNCC	1 cut(s)	2042
NmeAIII	GCCGAG	1 cut(s)	525
NmuCI	GTSAC	2 cut(s)	47, 1166
NruI	TCGCGA	1 cut(s)	462
NsiI	ATGCAT	2 cut(s)	6, 598
NspI	RCATGY	4 cut(s)	589, 1277, 2054, 2369
PalAI	GGCGCGCC	1 cut(s)	764
PaqCI	CACCTGC	1 cut(s)	785
PasI	CCCWGGG	1 cut(s)	558
PauI	GCGCGC	1 cut(s)	764
PceI	AGGCCT	1 cut(s)	375
PciI	ACATGT	1 cut(s)	2365
PciSI	GCTCTTC	1 cut(s)	1665
PcsI	WCGNNNNNNNCGW	5 cut(s)	93, 119, 435, 459, 810
PctI	GAATGC	1 cut(s)	661
PdiI	GCCGGC	1 cut(s)	1835
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	8 cut(s)	78, 110, 1020, 1330, 1620, 1895, 2326, 2510
Ple19I	CGATCG	1 cut(s)	493
PleI	GAGTC	5 cut(s)	53, 178, 609, 818, 2158
PpsI	GAGTC	5 cut(s)	53, 178, 609, 818, 2158
Ppu21I	YACGTR	2 cut(s)	295, 2138
PpuMI	RGGWCCY	1 cut(s)	2040
PscI	ACATGT	1 cut(s)	2365
Psp124BI	GAGCTC	1 cut(s)	102
Psp1406I	AACGTT	1 cut(s)	1990
Psp5II	RGGWCCY	1 cut(s)	2040
Psp6I	CCWGG	1 cut(s)	557
PspEI	GGTNACC	1 cut(s)	47
PspFI	CCCAGC	2 cut(s)	1642, 1737
PspGI	CCWGG	1 cut(s)	557
PspN4I	GGNNCC	1 cut(s)	2042
PspPI	GGNCC	4 cut(s)	736, 1414, 2040, 2293
PspPPI	RGGWCCY	1 cut(s)	2040
PstI	CTGCAG	2 cut(s)	178, 1618
PsuI	RGATCY	2 cut(s)	799, 2011
PteI	GCGCGC	1 cut(s)	764
PvuI	CGATCG	1 cut(s)	493
PvuII	CAGCTG	2 cut(s)	405, 1085
RruI	TCGCGA	1 cut(s)	462
RsaI	GTAC	5 cut(s)	293, 637, 947, 2072, 2145
RsaNI	GTAC	5 cut(s)	292, 636, 946, 2071, 2144
Rsr2I	CGGWCCG	1 cut(s)	736
RsrII	CGGWCCG	1 cut(s)	736
SacI	GAGCTC	1 cut(s)	102
SalI	GTCGAC	1 cut(s)	665
SapI	GCTCTTC	1 cut(s)	1665
SaqAI	TTAA	3 cut(s)	579, 1470, 1602
Sau96I	GGNCC	4 cut(s)	736, 1414, 2040, 2293
SchI	GAGTC	5 cut(s)	53, 179, 610, 818, 2158
ScrFI	CCNGG	4 cut(s)	559, 858, 971, 1879
SduI	GDGCHC	4 cut(s)	13, 102, 976, 1613
SfaNI	GCATC	4 cut(s)	553, 2069, 2239, 2240
SfcI	CTRYAG	5 cut(s)	174, 1171, 1614, 1696, 2274
SgrDI	CGTCGACG	1 cut(s)	665
SgsI	GGCGCGCC	1 cut(s)	764
SinI	GGWCC	4 cut(s)	736, 1414, 2040, 2293
SmlI	CTYRAG	4 cut(s)	12, 518, 1856, 2553
SmoI	CTYRAG	4 cut(s)	12, 518, 1856, 2553
SseBI	AGGCCT	1 cut(s)	375
SspMI	CTAG	1 cut(s)	1982
SstI	GAGCTC	1 cut(s)	102
StuI	AGGCCT	1 cut(s)	375
StyD4I	CCNGG	4 cut(s)	557, 856, 969, 1877
StyI	CCWWGG	1 cut(s)	1245
TaiI	ACGT	5 cut(s)	297, 666, 1993, 2114, 2140
TatI	WGTACW	1 cut(s)	635
TauI	GCSGC	7 cut(s)	212, 411, 690, 879, 884, 895, 1364
TfiI	GAWTC	8 cut(s)	78, 110, 1020, 1330, 1620, 1895, 2326, 2510
Tru1I	TTAA	3 cut(s)	579, 1470, 1602
Tru9I	TTAA	3 cut(s)	579, 1470, 1602
TscAI	CASTG	3 cut(s)	199, 1284, 1672
TseFI	GTSAC	2 cut(s)	47, 1166
TseI	GCWGC	8 cut(s)	405, 780, 783, 1085, 1514, 1675, 2057, 2372
Tsp45I	GTSAC	2 cut(s)	47, 1166
TspGWI	ACGGA	4 cut(s)	708, 991, 1001, 2119
TspRI	CASTG	3 cut(s)	199, 1284, 1672
VpaK11BI	GGWCC	4 cut(s)	736, 1414, 2040, 2293
XagI	CCTNNNNNAGG	1 cut(s)	1856
XceI	RCATGY	4 cut(s)	589, 1277, 2054, 2369
XmiI	GTMKAC	4 cut(s)	330, 486, 666, 1874
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	1 cut(s)	1982
ZraI	GACGTC	2 cut(s)	664, 2112
Zsp2I	ATGCAT	2 cut(s)	6, 598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.