Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Forward (+)

37425534 .. 37435844

Gene Structure 10311 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG335700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGTCGAGGAGAGTAGTGGAGGACGAGGACGTGGACGAGGTTGGGGATGATGATGAGGAGGAAGAGAACGAGTATGAGGTGCAGGATCTGCGGGACCGGATCAAGTCGTCACGGGGAAGCAGATTCAATCTGATATCGAACGATTTGGGGCTGGATGGATCGTCGACGTCGACGACTCGGAGGAGAATGAGGAACTACCTGAGCCGAGAGAGCGTCATCAATGGCGTCAAAGGCCTCTCCAAGGGCATCGGCGTTATTCATCCAGACAACAGGTGGTATCGGGTATGGACAAAAGTGATACTAGTATGGGCAATCTACTCGTCCTTCTTCACACCATTCGAGTTCGGGTTTTTCAGGGGTTTGGAAGAGAGACTCTTTGTCCTAGACATCGTGGGTCAAGTAGCTTTCCTCGTTGACATTATTCTGCACTTCTTCCTCGCCTACAGGGACAGCCAGACCTATCGAATGGTCCATAAGCGCAACCTCATTGCTCTCCGGTATTTGAAATCTAGTTTCATCGTTGACTTGCTTGGTTGCATGCCTTGGGATAATATCTACAAGGCTTGTGGGAGAAGAGAGGAAGTGAGGTACCTTCTATGGATAAGGTTGAGTCGGGTGCGCAAAGTCACTAAGTTTTTCCGAGACCTTGAGAAGGATATTCGGATTAGTTATGAGTTTACCAGGATTGCAAAGCTTCTGGTTGTTGAGCTCTATTGCACACATACAGCAGCCTGCATCTTTTACTATTTGGCCACCACCTTGCCTCCTGTTGAAGAGGGGTACACATGGATTGGAAGTTTGAAGTTGGGTGACTATAGTTATTCAAACTTCAGAGATATCGATCTATGGAAGCGCTATACAACATCTTTATATTTTGCCATTGTAACCATGGCTACTGTTGGATATGGAGATATACATGCAGTAAACCTGAGGGAAATGATCTTCATAATGGTTTATGTTTCTTTTGACATGGTTCTTGGGGCTTATTTGATTGGTAACATGACAGCCTTAATTGTAAAAGGATCAAAGACAGAAAAGTTCAGGGATAAAATGACAGATCTTATCAAATATATGAACAGAAATAGACTTGGTAAGGATATCCGTAATCAGATCAAAGGCCACTTGCGTTTGCAGTATGAGAGTAACTACACGGAGGCTGCTGTTCTCCAGGACATCCCTGCTTCAATTCGTGCTAAGGTTACTAAGCTACATGAGGAATTTTTTCTTCCAGGAGAAGTGATAATGGAACCTGGAAATGTGGTAGATCAAGTTTATTTTGTCTGTCATGGTGTACTGGAGGAGGTGGGTTTAGGCGAAGATGGATCAGAAGAAACAGTTTCGCTTTTGCAACCTGACAGCTCATTTGGACAAGTCTCCATCCTTTGCAACATTCCCCAACCTTATACAGTTAGGGTATGTGAACTGTGCAGGCTTCTGCGACTGGATAAACAATCATTTACAAGTATCCTTGACATATACTTTTATGATGGGCGGAAAATCTTGAACAACCTTCTAGAGGGTAAAGGACCTCATATCAAGCAACTAGAATCAGACATTACTTTCCATATTGGAAAGCAAGAAGCTGAACTTGCTTTGAAGGTGAATAGTGCTGCTTATCATGGAGATCTGCATCAGCTAAAATGTTTGATCCGAGCTGGAGCTGATCCCAACAAGACAGATTACGATGGAAGGTCACCCTTGCATCTTGCTGCATTACGAGGGCATGAAGATATTACACTTTTCCTTATTCAGGAAGGAGTAGACATCAACATCAAAGACAATTTTGGGAACACACCCTTACTTGAATCCATCAAGAATGGTCATGATCGAGTTTCTTCTCTACTTATAAAAGAAGGGGCCTCCTTGAAGATAGAGAATGCTGGTAGTTTTTTATGTTCAGCAATAGCAAAGGGGGATTCAGATTTCCTTAAAAAGTTATTGTCAAACGGCATTGATCCGAACTCCAAGGGTTATGATCAACGGACCCCACTGCATATAGCTGCCTCAGAAGGACTATATTTGATGGCAAAGTTGCTGTTAGAAGCTGGGGCAAACGTTTTCTCAAAGGACAGATGGGGAAACACCCCACTAGATGAAGGTCGGATGTGTGGGAACAAGAATTTGATTAGATTACTGGAAGAAGCAAAGGATGCTCAGTTGTCGGAATTCCCATACCGTGCTCAAGAAATTGCAGATAAAATGCATCCGAAGAAATGCACAGTGTTTCCATTCCACCCATGGGATAGCAAAGAACACAGAAAACCTGGAATAGTGTTATGGGTCCCTGCCACTATTGAAAAGCTTATCGACACAGCGTCGGAGAAACTAGAATTTGAAGGTGGTATGTGTATATTATCAGAAGATGCAGGTAAAATTCTTGATGTAGGCTTGATAAATGATGGTCAGAAGCTGTATTTAGTCACCGAGACGCATTTGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

813

Amino Acids

92.81

Weight (kDa)

6.18

Isoelectric Point (pI)

38.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	93 - 343	9.6e-27	Ion transport protein
Ion_trans_2	PF07885	283 - 339	5e-08	Ion channel
cNMP_binding	PF00027	406 - 491	1.1e-16	Cyclic nucleotide-binding domain
Ank_2	PF12796	537 - 624	1e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	537 - 583	1.9e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	549 - 600	3.1e-09	Ankyrin repeats (many copies)
Ank	PF00023	562 - 594	5.2e-08	Ankyrin repeat
Ank_2	PF12796	632 - 713	2.9e-10	Ankyrin repeats (3 copies)
Ank_5	PF13857	646 - 698	1.6e-08	Ankyrin repeats (many copies)
Ank	PF00023	660 - 690	1.2e-06	Ankyrin repeat
KHA	PF11834	737 - 806	1.4e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1847
AatII	GACGTC	1 cut(s)	170
Acc16I	TGCGCA	1 cut(s)	620
Acc36I	ACCTGC	1 cut(s)	2357
Acc65I	GGTACC	1 cut(s)	588
AccB1I	GGYRCC	1 cut(s)	588
AccI	GTMKAC	3 cut(s)	164, 170, 1761
AciI	CCGC	2 cut(s)	91, 1492
AclI	AACGTT	1 cut(s)	2055
AclWI	GGATC	8 cut(s)	93, 107, 166, 1030, 1330, 1642, 1658, 1949
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	5 cut(s)	1217, 2119, 2165, 2330, 2373
AcuI	CTGAAG	1 cut(s)	814
AcyI	GRCGYC	2 cut(s)	167, 225
AfaI	GTAC	3 cut(s)	590, 782, 1293
AfeI	AGCGCT	1 cut(s)	854
AfiI	CCNNNNNNNGG	5 cut(s)	241, 652, 1516, 1750, 2239
AhdI	GACNNNNNGTC	2 cut(s)	377, 2314
AhlI	ACTAGT	1 cut(s)	301
AjiI	CACGTC	1 cut(s)	31
AjnI	CCWGG	5 cut(s)	680, 1167, 1228, 1249, 2263
Alw21I	GWGCWC	2 cut(s)	711, 2182
Alw26I	GTCTC	4 cut(s)	364, 636, 1378, 2420
AlwI	GGATC	8 cut(s)	93, 107, 166, 1030, 1330, 1642, 1658, 1949
AlwNI	CAGNNNCTG	2 cut(s)	88, 2410
Aor51HI	AGCGCT	1 cut(s)	854
AoxI	GGCC	4 cut(s)	232, 750, 1117, 1857
ApeKI	GCWGC	5 cut(s)	728, 1157, 1610, 1709, 2000
ApoI	RAATTY	5 cut(s)	1217, 2119, 2165, 2330, 2373
ArsI	GACNNNNNNTTYG	2 cut(s)	1346, 1378
Asp700I	GAANNNNTTC	1 cut(s)	1221
Asp718I	GGTACC	1 cut(s)	588
AspLEI	GCGC	3 cut(s)	480, 621, 855
AspS9I	GGNCC	6 cut(s)	94, 469, 1526, 1857, 1983, 2281
AsuHPI	GGTGA	4 cut(s)	821, 1612, 1686, 2413
AvaII	GGWCC	5 cut(s)	94, 469, 1526, 1983, 2281
AxyI	CCTNAGG	1 cut(s)	929
BalI	TGGCCA	1 cut(s)	752
BanI	GGYRCC	1 cut(s)	588
BanII	GRGCYC	1 cut(s)	711
Bbv12I	GWGCWC	2 cut(s)	711, 2182
BbvI	GCAGC	5 cut(s)	740, 1144, 1597, 1696, 1987
BccI	CCATC	9 cut(s)	149, 1313, 1385, 1481, 1679, 1817, 2017, 2067, 2393
BceAI	ACGGC	1 cut(s)	1963
BcgI	CGANNNNNNTGC	2 cut(s)	300, 334
BciT130I	CCWGG	5 cut(s)	682, 1169, 1230, 1251, 2265
BciVI	GTATCC	1 cut(s)	1475
BclI	TGATCA	1 cut(s)	1975
BcoDI	GTCTC	4 cut(s)	364, 636, 1378, 2420
BcuI	ACTAGT	1 cut(s)	301
BfaI	CTAG	7 cut(s)	302, 383, 510, 1514, 1544, 2090, 2327
BfmI	CTRYAG	2 cut(s)	442, 814
BfoI	RGCGCY	1 cut(s)	856
BfuAI	ACCTGC	1 cut(s)	2357
BfuI	GTATCC	1 cut(s)	1475
BglII	AGATCT	2 cut(s)	1057, 1624
BisI	GCNGC	5 cut(s)	729, 1158, 1611, 1710, 2001
BlsI	GCNGC	5 cut(s)	730, 1159, 1612, 1711, 2002
Bme1390I	CCNGG	5 cut(s)	682, 1169, 1230, 1251, 2265
Bme18I	GGWCC	5 cut(s)	94, 469, 1526, 1983, 2281
BmeRI	GACNNNNNGTC	2 cut(s)	377, 2314
BmgBI	CACGTC	1 cut(s)	31
BmgT120I	GGNCC	6 cut(s)	94, 469, 1526, 1857, 1983, 2281
BmiI	GGNNCC	7 cut(s)	95, 590, 1248, 1858, 1985, 2282, 2283
BmrFI	CCNGG	5 cut(s)	682, 1169, 1230, 1251, 2265
BmsI	GCATC	7 cut(s)	255, 744, 1639, 1711, 2140, 2212, 2353
BpmI	CTGGAG	3 cut(s)	1151, 1316, 1677
Bpu10I	CCTNAGC	2 cut(s)	200, 1194
BpuEI	CTTGAG	2 cut(s)	668, 2166
Bsa29I	ATCGAT	1 cut(s)	840
BsaBI	GATNNNNATC	2 cut(s)	840, 1629
BsaHI	GRCGYC	2 cut(s)	167, 225
BsaI	GGTCTC	1 cut(s)	636
BsaJI	CCNNGG	5 cut(s)	240, 542, 888, 1965, 2237
BsaWI	WCCGGW	2 cut(s)	96, 495
BsaXI	ACNNNNNCTCC	2 cut(s)	748, 778
Bsc4I	CCNNNNNNNGG	5 cut(s)	241, 652, 1516, 1750, 2239
Bse1I	ACTGG	3 cut(s)	1299, 1446, 2139
Bse21I	CCTNAGG	1 cut(s)	929
Bse3DI	GCAATG	1 cut(s)	486
Bse8I	GATNNNNATC	2 cut(s)	840, 1629
BseBI	CCWGG	5 cut(s)	682, 1169, 1230, 1251, 2265
BseCI	ATCGAT	1 cut(s)	840
BseDI	CCNNGG	5 cut(s)	240, 542, 888, 1965, 2237
BseGI	GGATG	8 cut(s)	52, 160, 259, 1173, 1377, 2109, 2155, 2203
BseJI	GATNNNNATC	2 cut(s)	840, 1629
BseLI	CCNNNNNNNGG	5 cut(s)	241, 652, 1516, 1750, 2239
BseMI	GCAATG	1 cut(s)	486
BseMII	CTCAG	4 cut(s)	191, 920, 2019, 2168
BseNI	ACTGG	3 cut(s)	1299, 1446, 2139
BseRI	GAGGAG	4 cut(s)	22, 71, 196, 1313
BseXI	GCAGC	5 cut(s)	740, 1144, 1597, 1696, 1987
BseYI	CCCAGC	1 cut(s)	2045
BsgI	GTGCAG	3 cut(s)	101, 410, 1447
BshFI	GGCC	4 cut(s)	234, 752, 1119, 1859
BshNI	GGYRCC	1 cut(s)	588
BshVI	ATCGAT	1 cut(s)	840
BsiHKAI	GWGCWC	2 cut(s)	711, 2182
BsiSI	CCGG	2 cut(s)	97, 496
BslFI	GGGAC	3 cut(s)	107, 461, 2267
BslI	CCNNNNNNNGG	5 cut(s)	241, 652, 1516, 1750, 2239
BsmAI	GTCTC	4 cut(s)	364, 636, 1378, 2420
BsmBI	CGTCTC	1 cut(s)	2420
BsmFI	GGGAC	3 cut(s)	107, 461, 2267
BsmI	GAATGC	1 cut(s)	1882
BsnI	GGCC	4 cut(s)	234, 752, 1119, 1859
Bso31I	GGTCTC	1 cut(s)	636
Bsp1286I	GDGCHC	2 cut(s)	711, 2182
Bsp19I	CCATGG	2 cut(s)	888, 2237
BspACI	CCGC	2 cut(s)	91, 1492
BspANI	GGCC	4 cut(s)	234, 752, 1119, 1859
BspCNI	CTCAG	4 cut(s)	192, 921, 2018, 2167
BspDI	ATCGAT	1 cut(s)	840
BspHI	TCATGA	1 cut(s)	1822
BspLI	GGNNCC	7 cut(s)	95, 590, 1248, 1858, 1985, 2282, 2283
BspMI	ACCTGC	1 cut(s)	2357
BspPI	GGATC	8 cut(s)	93, 107, 166, 1030, 1330, 1642, 1658, 1949
BspT107I	GGYRCC	1 cut(s)	588
BspTNI	GGTCTC	1 cut(s)	636
BsrDI	GCAATG	1 cut(s)	486
BsrI	ACTGG	3 cut(s)	1299, 1446, 2139
BssECI	CCNNGG	5 cut(s)	240, 542, 888, 1965, 2237
BssNI	GRCGYC	2 cut(s)	167, 225
BssT1I	CCWWGG	5 cut(s)	240, 542, 888, 1965, 2237
Bst2UI	CCWGG	5 cut(s)	682, 1169, 1230, 1251, 2265
Bst4CI	ACNGT	6 cut(s)	898, 1336, 1408, 1425, 2177, 2221
Bst6I	CTCTTC	4 cut(s)	57, 360, 568, 768
BstACI	GRCGYC	2 cut(s)	167, 225
BstAPI	GCANNNNNTGC	2 cut(s)	88, 2150
BstC8I	GCNNGC	3 cut(s)	539, 733, 1430
BstDEI	CTNAG	7 cut(s)	200, 630, 929, 1194, 1203, 2005, 2154
BstDSI	CCRYGG	2 cut(s)	888, 2237
BstEII	GGTNACC	1 cut(s)	1692
BstENI	CCTNNNNNAGG	4 cut(s)	239, 650, 1514, 1748
BstF5I	GGATG	8 cut(s)	52, 160, 259, 1173, 1377, 2109, 2155, 2203
BstH2I	RGCGCY	1 cut(s)	856
BstHHI	GCGC	3 cut(s)	480, 621, 855
BstMAI	GTCTC	4 cut(s)	364, 636, 1378, 2420
BstMWI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1589, 2150
BstNI	CCWGG	5 cut(s)	682, 1169, 1230, 1251, 2265
BstNSI	RCATGY	2 cut(s)	541, 920
BstPI	GGTNACC	1 cut(s)	1692
BstSCI	CCNGG	5 cut(s)	680, 1167, 1228, 1249, 2263
BstSFI	CTRYAG	2 cut(s)	442, 814
BstV1I	GCAGC	5 cut(s)	740, 1144, 1597, 1696, 1987
BstX2I	RGATCY	3 cut(s)	85, 1057, 1624
BstYI	RGATCY	3 cut(s)	85, 1057, 1624
Bsu15I	ATCGAT	1 cut(s)	840
Bsu36I	CCTNAGG	1 cut(s)	929
BsuI	GTATCC	1 cut(s)	1475
BsuRI	GGCC	4 cut(s)	234, 752, 1119, 1859
BsuTUI	ATCGAT	1 cut(s)	840
BtgI	CCRYGG	2 cut(s)	888, 2237
BtrI	CACGTC	1 cut(s)	31
BtsCI	GGATG	8 cut(s)	52, 160, 259, 1173, 1377, 2109, 2155, 2203
BtsI	GCAGTG	1 cut(s)	1988
BtsIMutI	CAGTG	2 cut(s)	1988, 2226
BveI	ACCTGC	1 cut(s)	2357
Cac8I	GCNNGC	3 cut(s)	539, 733, 1430
CaiI	CAGNNNCTG	2 cut(s)	88, 2410
CciI	TCATGA	1 cut(s)	1822
CfoI	GCGC	3 cut(s)	480, 621, 855
Cfr13I	GGNCC	6 cut(s)	94, 469, 1526, 1857, 1983, 2281
ClaI	ATCGAT	1 cut(s)	840
CseI	GACGC	4 cut(s)	202, 214, 2304, 2437
Csp6I	GTAC	3 cut(s)	589, 781, 1292
CspCI	CAANNNNNGTGG	2 cut(s)	547, 582
CviQI	GTAC	3 cut(s)	589, 781, 1292
DdeI	CTNAG	7 cut(s)	200, 630, 929, 1194, 1203, 2005, 2154
DriI	GACNNNNNGTC	2 cut(s)	377, 2314
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	4 cut(s)	57, 360, 568, 768
Eam1105I	GACNNNNNGTC	2 cut(s)	377, 2314
EarI	CTCTTC	4 cut(s)	57, 360, 568, 768
EciI	GGCGGA	1 cut(s)	1507
Ecl136II	GAGCTC	1 cut(s)	709
Eco130I	CCWWGG	5 cut(s)	240, 542, 888, 1965, 2237
Eco147I	AGGCCT	1 cut(s)	234
Eco24I	GRGCYC	1 cut(s)	711
Eco31I	GGTCTC	1 cut(s)	636
Eco32I	GATATC	3 cut(s)	135, 838, 1099
Eco47I	GGWCC	5 cut(s)	94, 469, 1526, 1983, 2281
Eco47III	AGCGCT	1 cut(s)	854
Eco53kI	GAGCTC	1 cut(s)	709
Eco57I	CTGAAG	1 cut(s)	814
Eco81I	CCTNAGG	1 cut(s)	929
Eco91I	GGTNACC	1 cut(s)	1692
EcoICRI	GAGCTC	1 cut(s)	709
EcoNI	CCTNNNNNAGG	4 cut(s)	239, 650, 1514, 1748
EcoO109I	RGGNCCY	3 cut(s)	1526, 1857, 2281
EcoO65I	GGTNACC	1 cut(s)	1692
EcoRI	GAATTC	1 cut(s)	2165
EcoRII	CCWGG	5 cut(s)	680, 1167, 1228, 1249, 2263
EcoRV	GATATC	3 cut(s)	135, 838, 1099
EcoT14I	CCWWGG	5 cut(s)	240, 542, 888, 1965, 2237
EcoT22I	ATGCAT	1 cut(s)	2205
EcoT38I	GRGCYC	1 cut(s)	711
ErhI	CCWWGG	5 cut(s)	240, 542, 888, 1965, 2237
Esp3I	CGTCTC	1 cut(s)	2420
FalI	AAGNNNNNCTT	2 cut(s)	1572, 1604
FaqI	GGGAC	3 cut(s)	107, 461, 2267
FauI	CCCGC	1 cut(s)	84
FbaI	TGATCA	1 cut(s)	1975
FblI	GTMKAC	3 cut(s)	164, 170, 1761
Fnu4HI	GCNGC	5 cut(s)	729, 1158, 1611, 1710, 2001
FokI	GGATG	8 cut(s)	59, 167, 246, 1160, 1364, 2116, 2162, 2190
FriOI	GRGCYC	1 cut(s)	711
Fsp4HI	GCNGC	5 cut(s)	729, 1158, 1611, 1710, 2001
FspBI	CTAG	7 cut(s)	302, 383, 510, 1514, 1544, 2090, 2327
FspI	TGCGCA	1 cut(s)	620
GlaI	GCGC	3 cut(s)	479, 620, 854
GluI	GCNGC	5 cut(s)	729, 1158, 1611, 1710, 2001
GsaI	CCCAGC	1 cut(s)	2049
GsuI	CTGGAG	3 cut(s)	1151, 1316, 1677
HaeII	RGCGCY	1 cut(s)	856
HaeIII	GGCC	4 cut(s)	234, 752, 1119, 1859
HapII	CCGG	2 cut(s)	97, 496
HgaI	GACGC	4 cut(s)	202, 214, 2304, 2437
HhaI	GCGC	3 cut(s)	480, 621, 855
Hin1I	GRCGYC	2 cut(s)	167, 225
Hin6I	GCGC	3 cut(s)	478, 619, 853
HinP1I	GCGC	3 cut(s)	478, 619, 853
HincII	GTYRAC	4 cut(s)	165, 171, 415, 523
HindII	GTYRAC	4 cut(s)	165, 171, 415, 523
HindIII	AAGCTT	2 cut(s)	692, 2300
HinfI	GANTC	7 cut(s)	123, 175, 372, 610, 1547, 1805, 1916
HpaII	CCGG	2 cut(s)	97, 496
HphI	GGTGA	4 cut(s)	821, 1612, 1686, 2413
Hpy188III	TCNNGA	8 cut(s)	263, 1501, 1514, 1751, 1813, 1823, 2183, 2378
Hpy99I	CGWCG	5 cut(s)	166, 169, 172, 175, 2320
HpyCH4III	ACNGT	6 cut(s)	898, 1336, 1408, 1425, 2177, 2221
HpyCH4IV	ACGT	3 cut(s)	30, 167, 2055
HpyF10VI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1589, 2150
HpyF3I	CTNAG	7 cut(s)	200, 630, 929, 1194, 1203, 2005, 2154
HpySE526I	ACGT	3 cut(s)	30, 167, 2055
Hsp92I	GRCGYC	2 cut(s)	167, 225
HspAI	GCGC	3 cut(s)	478, 619, 853
KflI	GGGWCCC	1 cut(s)	2281
KpnI	GGTACC	1 cut(s)	592
Ksp22I	TGATCA	1 cut(s)	1975
LmnI	GCTCC	1 cut(s)	1658
Lsp1109I	GCAGC	5 cut(s)	740, 1144, 1597, 1696, 1987
LweI	GCATC	7 cut(s)	255, 744, 1639, 1711, 2140, 2212, 2353
MaeI	CTAG	7 cut(s)	302, 383, 510, 1514, 1544, 2090, 2327
MaeII	ACGT	3 cut(s)	30, 167, 2055
MaeIII	GTNAC	9 cut(s)	108, 625, 809, 883, 995, 1142, 1198, 1692, 2419
MflI	RGATCY	3 cut(s)	85, 1057, 1624
MhlI	GDGCHC	2 cut(s)	711, 2182
MlsI	TGGCCA	1 cut(s)	752
MluCI	AATT	9 cut(s)	1011, 1185, 1217, 1780, 2119, 2165, 2187, 2330, 2373
MluNI	TGGCCA	1 cut(s)	752
MlyI	GAGTC	3 cut(s)	169, 366, 619
MmeI	TCCRAC	4 cut(s)	880, 2081, 2142, 2298
Mox20I	TGGCCA	1 cut(s)	752
Mph1103I	ATGCAT	1 cut(s)	2205
MroXI	GAANNNNTTC	1 cut(s)	1221
MscI	TGGCCA	1 cut(s)	752
MseI	TTAA	2 cut(s)	1010, 1929
Msp20I	TGGCCA	1 cut(s)	752
MspI	CCGG	2 cut(s)	97, 496
MspR9I	CCNGG	5 cut(s)	682, 1169, 1230, 1251, 2265
Mva1269I	GAATGC	1 cut(s)	1882
MvaI	CCWGG	5 cut(s)	682, 1169, 1230, 1251, 2265
MwoI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1589, 2150
NcoI	CCATGG	2 cut(s)	888, 2237
NlaIV	GGNNCC	7 cut(s)	95, 590, 1248, 1858, 1985, 2282, 2283
NmeAIII	GCCGAG	1 cut(s)	230
NmuCI	GTSAC	5 cut(s)	108, 625, 809, 1692, 2419
NsbI	TGCGCA	1 cut(s)	620
NsiI	ATGCAT	1 cut(s)	2205
NspI	RCATGY	2 cut(s)	541, 920
PaeI	GCATGC	1 cut(s)	541
PagI	TCATGA	1 cut(s)	1822
PceI	AGGCCT	1 cut(s)	234
PcsI	WCGNNNNNNNCGW	2 cut(s)	167, 170
PctI	GAATGC	1 cut(s)	1882
PdmI	GAANNNNTTC	1 cut(s)	1221
PfeI	GAWTC	4 cut(s)	123, 1547, 1805, 1916
PfoI	TCCNGGA	2 cut(s)	1167, 1228
PkrI	GCNGC	5 cut(s)	730, 1159, 1612, 1711, 2002
PleI	GAGTC	3 cut(s)	169, 366, 618
PpsI	GAGTC	3 cut(s)	169, 366, 618
PpuMI	RGGWCCY	2 cut(s)	1526, 2281
PsiI	TTATAA	1 cut(s)	1847
Psp124BI	GAGCTC	1 cut(s)	711
Psp1406I	AACGTT	1 cut(s)	2055
Psp5II	RGGWCCY	2 cut(s)	1526, 2281
Psp6I	CCWGG	5 cut(s)	680, 1167, 1228, 1249, 2263
PspEI	GGTNACC	1 cut(s)	1692
PspFI	CCCAGC	1 cut(s)	2045
PspGI	CCWGG	5 cut(s)	680, 1167, 1228, 1249, 2263
PspN4I	GGNNCC	7 cut(s)	95, 590, 1248, 1858, 1985, 2282, 2283
PspPI	GGNCC	6 cut(s)	94, 469, 1526, 1857, 1983, 2281
PspPPI	RGGWCCY	2 cut(s)	1526, 2281
PstNI	CAGNNNCTG	2 cut(s)	88, 2410
PsuI	RGATCY	3 cut(s)	85, 1057, 1624
RsaI	GTAC	3 cut(s)	590, 782, 1293
RsaNI	GTAC	3 cut(s)	589, 781, 1292
SacI	GAGCTC	1 cut(s)	711
SalI	GTCGAC	2 cut(s)	163, 169
SaqAI	TTAA	2 cut(s)	1010, 1929
SatI	GCNGC	5 cut(s)	729, 1158, 1611, 1710, 2001
Sau96I	GGNCC	6 cut(s)	94, 469, 1526, 1857, 1983, 2281
SchI	GAGTC	3 cut(s)	169, 366, 619
ScrFI	CCNGG	5 cut(s)	682, 1169, 1230, 1251, 2265
SduI	GDGCHC	2 cut(s)	711, 2182
SfaNI	GCATC	7 cut(s)	255, 744, 1639, 1711, 2140, 2212, 2353
SfcI	CTRYAG	2 cut(s)	442, 814
SgrDI	CGTCGACG	2 cut(s)	163, 169
SinI	GGWCC	5 cut(s)	94, 469, 1526, 1983, 2281
SmlI	CTYRAG	2 cut(s)	647, 2181
SmoI	CTYRAG	2 cut(s)	647, 2181
SpeI	ACTAGT	1 cut(s)	301
SphI	GCATGC	1 cut(s)	541
Sse9I	AATT	9 cut(s)	1011, 1185, 1217, 1780, 2119, 2165, 2187, 2330, 2373
SseBI	AGGCCT	1 cut(s)	234
SsiI	CCGC	2 cut(s)	91, 1492
SspMI	CTAG	7 cut(s)	302, 383, 510, 1514, 1544, 2090, 2327
SstI	GAGCTC	1 cut(s)	711
StuI	AGGCCT	1 cut(s)	234
StyD4I	CCNGG	5 cut(s)	680, 1167, 1228, 1249, 2263
StyI	CCWWGG	5 cut(s)	240, 542, 888, 1965, 2237
TaaI	ACNGT	6 cut(s)	898, 1336, 1408, 1425, 2177, 2221
TaiI	ACGT	3 cut(s)	33, 170, 2058
TaqI	TCGA	9 cut(s)	5, 137, 164, 170, 339, 463, 840, 1828, 2307
TasI	AATT	9 cut(s)	1011, 1185, 1217, 1780, 2119, 2165, 2187, 2330, 2373
TatI	WGTACW	1 cut(s)	1291
TfiI	GAWTC	4 cut(s)	123, 1547, 1805, 1916
Tru1I	TTAA	2 cut(s)	1010, 1929
Tru9I	TTAA	2 cut(s)	1010, 1929
TscAI	CASTG	2 cut(s)	1995, 2226
TseFI	GTSAC	5 cut(s)	108, 625, 809, 1692, 2419
TseI	GCWGC	5 cut(s)	728, 1157, 1610, 1709, 2000
Tsp45I	GTSAC	5 cut(s)	108, 625, 809, 1692, 2419
TspDTI	ATGAA	6 cut(s)	248, 505, 934, 1088, 1740, 2109
TspGWI	ACGGA	3 cut(s)	1091, 1166, 1996
TspRI	CASTG	2 cut(s)	1995, 2226
VpaK11BI	GGWCC	5 cut(s)	94, 469, 1526, 1983, 2281
XagI	CCTNNNNNAGG	4 cut(s)	239, 650, 1514, 1748
XapI	RAATTY	5 cut(s)	1217, 2119, 2165, 2330, 2373
XbaI	TCTAGA	1 cut(s)	1513
XceI	RCATGY	2 cut(s)	541, 920
XcmI	CCANNNNNNNNNTGG	3 cut(s)	270, 886, 2235
XmiI	GTMKAC	3 cut(s)	164, 170, 1761
XmnI	GAANNNNTTC	1 cut(s)	1221
XspI	CTAG	7 cut(s)	302, 383, 510, 1514, 1544, 2090, 2327
ZraI	GACGTC	1 cut(s)	168
Zsp2I	ATGCAT	1 cut(s)	2205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.