Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Reverse (-)

31093260 .. 31100124

Gene Structure 6865 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1255200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGTCGAGGCGAGTGGTGGTGGAGGACCACCGTCACACGGAGGGTGAGTCGTTGAAGGACGACGCCGAAGAGGAGGATGAAGAGTATCAGGTGCAGGATCTGAGGGAGCGAATCAAATCGTCGCTCGGGAGCCAATTCAATCTGATTAAGAACGAGTTAGGACTCGGAGACGGTTCTTCGACACGGCGGCGGCGGTTCAGTCGCCAGAGCCTGATCGATGGCGTCAGGGGGCTCTCCAAAGGATTCGTCGTTCATCCTGACAACTGGTGGTATCGGGCATGGATGATTTTCATACTTGTATGGGCAGTGTACTCGTCCTTCTTCACACCTTTCGAGTTCGGGTTTTTCAGGGGTTTGGAAAAGAAACTTTTTATACTGGATGTAGTAGGACAGATAGCATTTCTTGTAGACATTGTTTTGCAGTTCTTCCTTGCCTACCGGGACAGTAACACCTATCGAATGGTCTATAACCGCAAACGGATTGCTCTGAGGTACTTGAAATCGAGTTTTATCATTGACTTGCTTGGTTGTATGCCTTGGGATCTCATCTACAAGGCTAGTGGGAGAAGAGAGGAAGTTAGGTATCTCCTATGGATAAGGCTATATCGGTTGTGCAAAGTCACCAAGTTCCTTCATGACCTAGAGAAGGATATTCGTATCAGTTATCAGTTTACCAGAATTATAAAGCTTATTGTCGTTGAACTCTACTGCACCCATACAGCGGCCTGCATCTTTTATTATTTGGCCACCACCCTGCCTGCTTCACAAGAGGGGTATACATGGATTGGAAGTTTGAAGTTAGGTGACTATAGTTATTCAAGCTTCAGGGACATCGACTTGTGGAAGCGCTATACAACTTCATTGTATTTTGCCATTGTAACCATGGCCACTGTTGGTTATGGAGATATTCATGCAGTCAACCTGAGGGAAATGATATTCATAATGGTTTATGTATCTTTTGACATGGTTCTTGGAGCGTATTTAATTGGTAACATGACAGCGTTAATTGTAAAAGGATCAAAGACAGAAAAGTTCAGGGACAAAATGACAGATATTATGAAATATATGAACAGAAATAGACTTGGGAGGGATATCCGTAATCAAATCAAAGGTCACTTGCGTTTACAATATGAGAGTACCTACACGGAGGCTGCTGTTCTCCAGGAAATTCCTGCTTCAATTCGCGCTAAGATTTCACAAACTTTATATTTCCCATACATTGAAAGTGTTCCTATCTTCAAGGGATGCTCGACAGAATTCATCAATCAGATCGTTATTAAACTTCATGAAGAATTTTTCCTCCCTGGAGAAGTGATCATGGAACCAGGAAACGTCATAGATCAACTTTATTTCGTCTGTCATGGTGTGCTGGAGGAGGTAGGCATAGGGGAAGATGGAACAGAAGAAATTGTTGAGCGATTGGAACCAAACAGCACATTTGGAGAAATTTCCATTCTTTGCAACATTCCTCAACTTCATACAGTTAGGGTGTGTGAATTATGTAGACTTCTGCGACTTGATAAGCAATCTTTGACAAGTATCCTTGACATATACTTCTATGACGGGCGGAAAATCTTGAACAACCTTTTAGAGGGTAAAGCACCTCGCATGAAACAACTAGAGTCGGACATTACATTTCATATCGGAAAGCAAGAAGCTGAACTTGCTTTGAAGGTCAATAGCGCAGCTTACCACGGAGATCTATTTCAGCTGAAAGGTTTGATCCGAACTGGAGCTGATCCCAACAAGGCAGATTATGATGGAAGGTCACCTTTGCATCTCGCTGCATTAAAAGGACATGAAGATATTACACTTTTCCTTATTCAGGAAGGAGTAGACATCAACATCAAAGACAATTTTGGGAACACACCTTTACTTGAAGCCATCAAGAACGGGAATGATCGAGTTTCTTCTTTACTTATTCAAGAAGGGGCCTCCTTGAACATAGACAATGCTGGTAGTTTTATATGTACATCCGTTGCAAGGGGGGACACAGATCTGCTTAAAAGGTTATTGGCAAATGGCATTGACCCAAATTCAAAAGATTCTGATCACCGGACTCCACTTCATGTAGCTGCATCAGAAGGACTATATGTGATGGCAAAGTTGCTGTTAGAATCTGGGGCTAGCGTCTTCCCAAAGGACAGATGGGGAAATACCCCACTAGATGAAAGTCGGATGTGTGGGAACAAGAATTTAATTAAACTGCTGGAAGAAGCAAAGTCAGCTCAGTCGATAGAATTCCCTTGCAATGCTCAAGAAAACACAGATAAAATGCACCGGAAGAAGTGCACAGTATACCCTTTCCACCCATGGGAAAGCAAACAAGAAAGAAGACCTGGAATCGTGTTATGGGTCCCGACTACCATTGGAGAACTCATCAGAACAGCAGGAGAGCAACTAGAGTTTTCATCGAGCAGTTCCTGCATATTATTAACAGAAGATGCAGGTAAAATTCTTGATGTGGACTTGATAAATGATGGTCAGAAGCTGTATTTGGTCAGTGAGACGCATTTTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

841

Amino Acids

96.15

Weight (kDa)

6.23

Isoelectric Point (pI)

41.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	91 - 341	3.7e-28	Ion transport protein
Ion_trans_2	PF07885	281 - 337	4.7e-08	Ion channel
cNMP_binding	PF00027	431 - 516	9e-17	Cyclic nucleotide-binding domain
Ank_2	PF12796	562 - 650	6.1e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	562 - 608	4.2e-08	Ankyrin repeats (many copies)
Ank_5	PF13857	574 - 625	2.1e-08	Ankyrin repeats (many copies)
Ank	PF00023	587 - 619	6.6e-08	Ankyrin repeat
Ank_4	PF13637	595 - 639	1.4e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	660 - 737	2.4e-11	Ankyrin repeats (3 copies)
Ank_4	PF13637	661 - 696	1.2e-06	Ankyrin repeats (many copies)
Ank_5	PF13857	671 - 723	6.1e-08	Ankyrin repeats (many copies)
KHA	PF11834	762 - 833	6.2e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	683
Acc36I	ACCTGC	1 cut(s)	2438
AccI	GTMKAC	5 cut(s)	408, 776, 1504, 1836, 2298
AccII	CGCG	1 cut(s)	1185
AciI	CCGC	6 cut(s)	187, 190, 193, 472, 722, 1567
AclWI	GGATC	5 cut(s)	105, 549, 1024, 1717, 1733
AcoI	YGGCCR	2 cut(s)	744, 885
AcsI	RAATTY	8 cut(s)	1167, 1256, 1292, 1446, 2035, 2194, 2240, 2454
AcuI	CTGAAG	1 cut(s)	808
AcyI	GRCGYC	2 cut(s)	63, 222
AfaI	GTAC	4 cut(s)	311, 494, 1138, 1972
AfeI	AGCGCT	1 cut(s)	848
AfiI	CCNNNNNNNGG	7 cut(s)	37, 646, 721, 1591, 1825, 1983, 2314
AjnI	CCWGG	4 cut(s)	1161, 1303, 1324, 2338
AjuI	GAANNNNNNNTTGG	2 cut(s)	2480, 2512
AloI	GAACNNNNNNTCC	2 cut(s)	234, 266
AluBI	AGCT	9 cut(s)	688, 822, 1658, 1688, 1711, 1736, 2075, 2228, 2491
AluI	AGCT	9 cut(s)	688, 822, 1658, 1688, 1711, 1736, 2075, 2228, 2491
Alw21I	GWGCWC	1 cut(s)	2294
Alw26I	GTCTC	2 cut(s)	162, 2501
Alw44I	GTGCAC	1 cut(s)	2290
AlwI	GGATC	5 cut(s)	105, 549, 1024, 1717, 1733
AlwNI	CAGNNNCTG	4 cut(s)	100, 211, 2424, 2491
Ama87I	CYCGRG	1 cut(s)	125
Aor51HI	AGCGCT	1 cut(s)	848
AoxI	GGCC	4 cut(s)	723, 744, 885, 1932
ApaLI	GTGCAC	1 cut(s)	2290
ApeKI	GCWGC	4 cut(s)	1151, 1685, 1784, 2075
ApoI	RAATTY	8 cut(s)	1167, 1256, 1292, 1446, 2035, 2194, 2240, 2454
ArsI	GACNNNNNNTTYG	4 cut(s)	231, 263, 1096, 1128
Asp700I	GAANNNNTTC	1 cut(s)	1227
AspLEI	GCGC	3 cut(s)	849, 1187, 1685
AspS9I	GGNCC	3 cut(s)	25, 1932, 2356
AsuC2I	CCSGG	1 cut(s)	440
AsuHPI	GGTGA	5 cut(s)	56, 613, 815, 1761, 2045
AsuNHI	GCTAGC	1 cut(s)	2126
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	2 cut(s)	25, 2356
AxyI	CCTNAGG	1 cut(s)	923
BaeGI	GKGCMC	1 cut(s)	2294
BalI	TGGCCA	2 cut(s)	746, 887
BanII	GRGCYC	1 cut(s)	234
BarI	GAAGNNNNNNTAC	2 cut(s)	302, 334
BbsI	GAAGAC	2 cut(s)	2125, 2341
Bbv12I	GWGCWC	1 cut(s)	2294
BbvI	GCAGC	4 cut(s)	1138, 1697, 1771, 2062
BccI	CCATC	7 cut(s)	212, 1388, 1754, 1892, 2092, 2142, 2474
BceAI	ACGGC	1 cut(s)	200
BcgI	CGANNNNNNTGC	2 cut(s)	294, 328
BciT130I	CCWGG	4 cut(s)	1163, 1305, 1326, 2340
BciVI	GTATCC	1 cut(s)	1550
BclI	TGATCA	2 cut(s)	1314, 2050
BcnI	CCSGG	1 cut(s)	440
BcoDI	GTCTC	2 cut(s)	162, 2501
BfaI	CTAG	6 cut(s)	558, 641, 1619, 2127, 2165, 2402
BfmI	CTRYAG	1 cut(s)	808
BfoI	RGCGCY	1 cut(s)	850
BfuAI	ACCTGC	1 cut(s)	2438
BfuI	GTATCC	1 cut(s)	1550
BglII	AGATCT	2 cut(s)	1699, 1996
BisI	GCNGC	7 cut(s)	188, 191, 723, 1152, 1686, 1785, 2076
BlsI	GCNGC	7 cut(s)	189, 192, 724, 1153, 1687, 1786, 2077
Bme1390I	CCNGG	5 cut(s)	440, 1163, 1305, 1326, 2340
Bme18I	GGWCC	2 cut(s)	25, 2356
BmeT110I	CYCGRG	1 cut(s)	125
BmgT120I	GGNCC	3 cut(s)	25, 1932, 2356
BmiI	GGNNCC	6 cut(s)	131, 1323, 1425, 1933, 2357, 2358
BmrFI	CCNGG	5 cut(s)	440, 1163, 1305, 1326, 2340
BmsI	GCATC	5 cut(s)	738, 1235, 1786, 2087, 2434
BmtI	GCTAGC	1 cut(s)	2130
BoxI	GACNNNNGTC	1 cut(s)	30
BpiI	GAAGAC	2 cut(s)	2125, 2341
BpmI	CTGGAG	4 cut(s)	1145, 1326, 1391, 1752
BpuEI	CTTGAG	1 cut(s)	2241
BpuMI	CCSGG	1 cut(s)	440
Bsa29I	ATCGAT	1 cut(s)	216
BsaBI	GATNNNNATC	1 cut(s)	2049
BsaHI	GRCGYC	2 cut(s)	63, 222
BsaJI	CCNNGG	5 cut(s)	536, 882, 1303, 1693, 2312
BsaWI	WCCGGW	2 cut(s)	2055, 2280
BsaXI	ACNNNNNCTCC	2 cut(s)	32, 62
Bsc4I	CCNNNNNNNGG	7 cut(s)	37, 646, 721, 1591, 1825, 1983, 2314
Bse1I	ACTGG	3 cut(s)	269, 381, 1735
Bse21I	CCTNAGG	1 cut(s)	923
Bse3DI	GCAATG	1 cut(s)	2257
Bse8I	GATNNNNATC	1 cut(s)	2049
BseBI	CCWGG	4 cut(s)	1163, 1305, 1326, 2340
BseCI	ATCGAT	1 cut(s)	216
BseDI	CCNNGG	5 cut(s)	536, 882, 1303, 1693, 2312
BseGI	GGATG	7 cut(s)	82, 253, 288, 385, 1250, 1973, 2184
BseJI	GATNNNNATC	1 cut(s)	2049
BseLI	CCNNNNNNNGG	7 cut(s)	37, 646, 721, 1591, 1825, 1983, 2314
BseMI	GCAATG	1 cut(s)	2257
BseMII	CTCAG	4 cut(s)	92, 479, 914, 2243
BseNI	ACTGG	3 cut(s)	269, 381, 1735
BseRI	GAGGAG	2 cut(s)	86, 1388
BseSI	GKGCMC	1 cut(s)	2294
BseXI	GCAGC	4 cut(s)	1138, 1697, 1771, 2062
BsgI	GTGCAG	2 cut(s)	113, 694
Bsh1236I	CGCG	1 cut(s)	1185
BshFI	GGCC	4 cut(s)	725, 746, 887, 1934
BshVI	ATCGAT	1 cut(s)	216
BsiHKAI	GWGCWC	1 cut(s)	2294
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	3 cut(s)	439, 2056, 2281
BslFI	GGGAC	5 cut(s)	455, 842, 1052, 2003, 2342
BslI	CCNNNNNNNGG	7 cut(s)	37, 646, 721, 1591, 1825, 1983, 2314
BsmAI	GTCTC	2 cut(s)	162, 2501
BsmBI	CGTCTC	2 cut(s)	162, 2501
BsmFI	GGGAC	5 cut(s)	455, 842, 1052, 2003, 2342
BsnI	GGCC	4 cut(s)	725, 746, 887, 1934
BsoBI	CYCGRG	1 cut(s)	125
Bsp1286I	GDGCHC	2 cut(s)	234, 2294
Bsp1407I	TGTACA	1 cut(s)	1970
Bsp19I	CCATGG	2 cut(s)	882, 2312
BspACI	CCGC	6 cut(s)	187, 190, 193, 472, 722, 1567
BspANI	GGCC	4 cut(s)	725, 746, 887, 1934
BspCNI	CTCAG	4 cut(s)	93, 480, 915, 2242
BspDI	ATCGAT	1 cut(s)	216
BspFNI	CGCG	1 cut(s)	1185
BspHI	TCATGA	2 cut(s)	634, 1285
BspLI	GGNNCC	6 cut(s)	131, 1323, 1425, 1933, 2357, 2358
BspMI	ACCTGC	1 cut(s)	2438
BspOI	GCTAGC	1 cut(s)	2130
BspPI	GGATC	5 cut(s)	105, 549, 1024, 1717, 1733
BsrDI	GCAATG	1 cut(s)	2257
BsrGI	TGTACA	1 cut(s)	1970
BsrI	ACTGG	3 cut(s)	269, 381, 1735
BssECI	CCNNGG	5 cut(s)	536, 882, 1303, 1693, 2312
BssNAI	GTATAC	2 cut(s)	777, 2299
BssNI	GRCGYC	2 cut(s)	63, 222
BssT1I	CCWWGG	3 cut(s)	536, 882, 2312
Bst1107I	GTATAC	2 cut(s)	777, 2299
Bst2UI	CCWGG	4 cut(s)	1163, 1305, 1326, 2340
Bst4CI	ACNGT	6 cut(s)	32, 173, 446, 892, 1483, 2296
Bst6I	CTCTTC	3 cut(s)	63, 75, 562
BstACI	GRCGYC	2 cut(s)	63, 222
BstAPI	GCANNNNNTGC	1 cut(s)	2424
BstAUI	TGTACA	1 cut(s)	1970
BstC8I	GCNNGC	3 cut(s)	727, 759, 2128
BstDEI	CTNAG	5 cut(s)	101, 488, 923, 1188, 2229
BstDSI	CCRYGG	3 cut(s)	882, 1693, 2312
BstEII	GGTNACC	1 cut(s)	1767
BstENI	CCTNNNNNAGG	3 cut(s)	644, 1589, 1823
BstF5I	GGATG	7 cut(s)	82, 253, 288, 385, 1250, 1973, 2184
BstFNI	CGCG	1 cut(s)	1185
BstH2I	RGCGCY	1 cut(s)	850
BstHHI	GCGC	3 cut(s)	849, 1187, 1685
BstMAI	GTCTC	2 cut(s)	162, 2501
BstMWI	GCNNNNNNNGC	3 cut(s)	1664, 2225, 2424
BstNI	CCWGG	4 cut(s)	1163, 1305, 1326, 2340
BstPAI	GACNNNNGTC	1 cut(s)	30
BstPI	GGTNACC	1 cut(s)	1767
BstSCI	CCNGG	5 cut(s)	438, 1161, 1303, 1324, 2338
BstSFI	CTRYAG	1 cut(s)	808
BstSLI	GKGCMC	1 cut(s)	2294
BstUI	CGCG	1 cut(s)	1185
BstV1I	GCAGC	4 cut(s)	1138, 1697, 1771, 2062
BstV2I	GAAGAC	2 cut(s)	2125, 2341
BstX2I	RGATCY	4 cut(s)	97, 541, 1699, 1996
BstYI	RGATCY	4 cut(s)	97, 541, 1699, 1996
BstZ17I	GTATAC	2 cut(s)	777, 2299
Bsu15I	ATCGAT	1 cut(s)	216
Bsu36I	CCTNAGG	1 cut(s)	923
BsuI	GTATCC	1 cut(s)	1550
BsuRI	GGCC	4 cut(s)	725, 746, 887, 1934
BsuTUI	ATCGAT	1 cut(s)	216
BtgI	CCRYGG	3 cut(s)	882, 1693, 2312
BtsCI	GGATG	7 cut(s)	82, 253, 288, 385, 1250, 1973, 2184
BtsI	GCAGTG	1 cut(s)	312
BtsIMutI	CAGTG	3 cut(s)	312, 888, 2509
BveI	ACCTGC	1 cut(s)	2438
Cac8I	GCNNGC	3 cut(s)	727, 759, 2128
CaiI	CAGNNNCTG	4 cut(s)	100, 211, 2424, 2491
CciI	TCATGA	2 cut(s)	634, 1285
CfoI	GCGC	3 cut(s)	849, 1187, 1685
Cfr13I	GGNCC	3 cut(s)	25, 1932, 2356
ClaI	ATCGAT	1 cut(s)	216
CseI	GACGC	4 cut(s)	71, 211, 2119, 2518
Csp6I	GTAC	4 cut(s)	310, 493, 1137, 1971
CspCI	CAANNNNNGTGG	2 cut(s)	541, 576
CviQI	GTAC	4 cut(s)	310, 493, 1137, 1971
DdeI	CTNAG	5 cut(s)	101, 488, 923, 1188, 2229
EaeI	YGGCCR	2 cut(s)	744, 885
Eam1104I	CTCTTC	3 cut(s)	63, 75, 562
EarI	CTCTTC	3 cut(s)	63, 75, 562
EciI	GGCGGA	1 cut(s)	1582
Eco130I	CCWWGG	3 cut(s)	536, 882, 2312
Eco24I	GRGCYC	1 cut(s)	234
Eco32I	GATATC	1 cut(s)	1093
Eco47I	GGWCC	2 cut(s)	25, 2356
Eco47III	AGCGCT	1 cut(s)	848
Eco57I	CTGAAG	1 cut(s)	808
Eco81I	CCTNAGG	1 cut(s)	923
Eco88I	CYCGRG	1 cut(s)	125
Eco91I	GGTNACC	1 cut(s)	1767
EcoNI	CCTNNNNNAGG	3 cut(s)	644, 1589, 1823
EcoO109I	RGGNCCY	2 cut(s)	1932, 2356
EcoO65I	GGTNACC	1 cut(s)	1767
EcoRI	GAATTC	2 cut(s)	1256, 2240
EcoRII	CCWGG	4 cut(s)	1161, 1303, 1324, 2338
EcoRV	GATATC	1 cut(s)	1093
EcoT14I	CCWWGG	3 cut(s)	536, 882, 2312
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	3 cut(s)	536, 882, 2312
Esp3I	CGTCTC	2 cut(s)	162, 2501
FalI	AAGNNNNNCTT	4 cut(s)	1647, 1679, 1756, 1788
FaqI	GGGAC	5 cut(s)	455, 842, 1052, 2003, 2342
FbaI	TGATCA	2 cut(s)	1314, 2050
FblI	GTMKAC	5 cut(s)	408, 776, 1504, 1836, 2298
Fnu4HI	GCNGC	7 cut(s)	188, 191, 723, 1152, 1686, 1785, 2076
FokI	GGATG	7 cut(s)	89, 240, 295, 392, 1257, 1960, 2191
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	7 cut(s)	188, 191, 723, 1152, 1686, 1785, 2076
FspBI	CTAG	6 cut(s)	558, 641, 1619, 2127, 2165, 2402
GlaI	GCGC	3 cut(s)	848, 1186, 1684
GluI	GCNGC	7 cut(s)	188, 191, 723, 1152, 1686, 1785, 2076
GsuI	CTGGAG	4 cut(s)	1145, 1326, 1391, 1752
HaeII	RGCGCY	1 cut(s)	850
HaeIII	GGCC	4 cut(s)	725, 746, 887, 1934
HapII	CCGG	3 cut(s)	439, 2056, 2281
HgaI	GACGC	4 cut(s)	71, 211, 2119, 2518
HhaI	GCGC	3 cut(s)	849, 1187, 1685
Hin1I	GRCGYC	2 cut(s)	63, 222
Hin6I	GCGC	3 cut(s)	847, 1185, 1683
HinP1I	GCGC	3 cut(s)	847, 1185, 1683
HincII	GTYRAC	1 cut(s)	919
HindII	GTYRAC	1 cut(s)	919
HindIII	AAGCTT	2 cut(s)	686, 820
HinfI	GANTC	9 cut(s)	47, 111, 162, 243, 1622, 2045, 2059, 2117, 2343
HpaII	CCGG	3 cut(s)	439, 2056, 2281
HphI	GGTGA	5 cut(s)	56, 613, 815, 1761, 2045
Hpy99I	CGWCG	3 cut(s)	65, 124, 251
HpyAV	CCTTC	9 cut(s)	49, 328, 640, 641, 1666, 1758, 1823, 1922, 2078
HpyCH4III	ACNGT	6 cut(s)	32, 173, 446, 892, 1483, 2296
HpyCH4IV	ACGT	1 cut(s)	1332
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1664, 2225, 2424
HpyF3I	CTNAG	5 cut(s)	101, 488, 923, 1188, 2229
HpySE526I	ACGT	1 cut(s)	1332
Hsp92I	GRCGYC	2 cut(s)	63, 222
HspAI	GCGC	3 cut(s)	847, 1185, 1683
KflI	GGGWCCC	1 cut(s)	2356
Ksp22I	TGATCA	2 cut(s)	1314, 2050
LmnI	GCTCC	4 cut(s)	106, 129, 974, 1733
Lsp1109I	GCAGC	4 cut(s)	1138, 1697, 1771, 2062
LweI	GCATC	5 cut(s)	738, 1235, 1786, 2087, 2434
MaeI	CTAG	6 cut(s)	558, 641, 1619, 2127, 2165, 2402
MaeII	ACGT	1 cut(s)	1332
MaeIII	GTNAC	8 cut(s)	32, 446, 619, 803, 877, 989, 1112, 1767
MflI	RGATCY	4 cut(s)	97, 541, 1699, 1996
MhlI	GDGCHC	2 cut(s)	234, 2294
MlsI	TGGCCA	2 cut(s)	746, 887
MluNI	TGGCCA	2 cut(s)	746, 887
MlyI	GAGTC	4 cut(s)	56, 156, 1631, 2053
MmeI	TCCRAC	2 cut(s)	1605, 2156
Mox20I	TGGCCA	2 cut(s)	746, 887
MroXI	GAANNNNTTC	1 cut(s)	1227
MscI	TGGCCA	2 cut(s)	746, 887
MseI	TTAA	9 cut(s)	147, 983, 1004, 1278, 1790, 2004, 2198, 2202, 2435
Msp20I	TGGCCA	2 cut(s)	746, 887
MspA1I	CMGCKG	2 cut(s)	722, 1711
MspI	CCGG	3 cut(s)	439, 2056, 2281
MspR9I	CCNGG	5 cut(s)	440, 1163, 1305, 1326, 2340
MvaI	CCWGG	4 cut(s)	1163, 1305, 1326, 2340
MvnI	CGCG	1 cut(s)	1185
MwoI	GCNNNNNNNGC	3 cut(s)	1664, 2225, 2424
NciI	CCSGG	1 cut(s)	440
NcoI	CCATGG	2 cut(s)	882, 2312
NheI	GCTAGC	1 cut(s)	2126
NlaIV	GGNNCC	6 cut(s)	131, 1323, 1425, 1933, 2357, 2358
NmuCI	GTSAC	5 cut(s)	32, 619, 803, 1112, 1767
PacI	TTAATTAA	1 cut(s)	2202
PagI	TCATGA	2 cut(s)	634, 1285
PdmI	GAANNNNTTC	1 cut(s)	1227
PfeI	GAWTC	5 cut(s)	111, 243, 2045, 2117, 2343
PfoI	TCCNGGA	1 cut(s)	1161
PkrI	GCNGC	7 cut(s)	189, 192, 724, 1153, 1687, 1786, 2077
PleI	GAGTC	4 cut(s)	55, 156, 1630, 2053
PpsI	GAGTC	4 cut(s)	55, 156, 1630, 2053
PpuMI	RGGWCCY	1 cut(s)	2356
PshAI	GACNNNNGTC	1 cut(s)	30
PsiI	TTATAA	1 cut(s)	683
Psp5II	RGGWCCY	1 cut(s)	2356
Psp6I	CCWGG	4 cut(s)	1161, 1303, 1324, 2338
PspEI	GGTNACC	1 cut(s)	1767
PspGI	CCWGG	4 cut(s)	1161, 1303, 1324, 2338
PspN4I	GGNNCC	6 cut(s)	131, 1323, 1425, 1933, 2357, 2358
PspPI	GGNCC	3 cut(s)	25, 1932, 2356
PspPPI	RGGWCCY	1 cut(s)	2356
PstNI	CAGNNNCTG	4 cut(s)	100, 211, 2424, 2491
PsuI	RGATCY	4 cut(s)	97, 541, 1699, 1996
PvuII	CAGCTG	1 cut(s)	1711
RsaI	GTAC	4 cut(s)	311, 494, 1138, 1972
RsaNI	GTAC	4 cut(s)	310, 493, 1137, 1971
SaqAI	TTAA	9 cut(s)	147, 983, 1004, 1278, 1790, 2004, 2198, 2202, 2435
SatI	GCNGC	7 cut(s)	188, 191, 723, 1152, 1686, 1785, 2076
Sau96I	GGNCC	3 cut(s)	25, 1932, 2356
SchI	GAGTC	4 cut(s)	56, 156, 1631, 2053
ScrFI	CCNGG	5 cut(s)	440, 1163, 1305, 1326, 2340
SduI	GDGCHC	2 cut(s)	234, 2294
SfaNI	GCATC	5 cut(s)	738, 1235, 1786, 2087, 2434
SfcI	CTRYAG	1 cut(s)	808
SinI	GGWCC	2 cut(s)	25, 2356
SmlI	CTYRAG	1 cut(s)	2256
SmoI	CTYRAG	1 cut(s)	2256
SsiI	CCGC	6 cut(s)	187, 190, 193, 472, 722, 1567
SspMI	CTAG	6 cut(s)	558, 641, 1619, 2127, 2165, 2402
StyD4I	CCNGG	5 cut(s)	438, 1161, 1303, 1324, 2338
StyI	CCWWGG	3 cut(s)	536, 882, 2312
TaaI	ACNGT	6 cut(s)	32, 173, 446, 892, 1483, 2296
TaiI	ACGT	1 cut(s)	1335
TatI	WGTACW	2 cut(s)	309, 1970
TauI	GCSGC	3 cut(s)	190, 193, 725
TfiI	GAWTC	5 cut(s)	111, 243, 2045, 2117, 2343
Tru1I	TTAA	9 cut(s)	147, 983, 1004, 1278, 1790, 2004, 2198, 2202, 2435
Tru9I	TTAA	9 cut(s)	147, 983, 1004, 1278, 1790, 2004, 2198, 2202, 2435
TscAI	CASTG	3 cut(s)	312, 895, 2509
TseFI	GTSAC	5 cut(s)	32, 619, 803, 1112, 1767
TseI	GCWGC	4 cut(s)	1151, 1685, 1784, 2075
Tsp45I	GTSAC	5 cut(s)	32, 619, 803, 1112, 1767
TspGWI	ACGGA	6 cut(s)	53, 493, 1085, 1160, 1710, 1966
TspRI	CASTG	3 cut(s)	312, 895, 2509
VneI	GTGCAC	1 cut(s)	2290
VpaK11BI	GGWCC	2 cut(s)	25, 2356
XagI	CCTNNNNNAGG	3 cut(s)	644, 1589, 1823
XapI	RAATTY	8 cut(s)	1167, 1256, 1292, 1446, 2035, 2194, 2240, 2454
XcmI	CCANNNNNNNNNTGG	2 cut(s)	880, 2145
XmiI	GTMKAC	5 cut(s)	408, 776, 1504, 1836, 2298
XmnI	GAANNNNTTC	1 cut(s)	1227
XspI	CTAG	6 cut(s)	558, 641, 1619, 2127, 2165, 2402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.