Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Forward (+)

36205539 .. 36216538

Gene Structure 11000 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG338200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGTCGAGGAGAGCAGTGGAGGACGAGGACGTGGACGAGGTTGGGGATGATGATGAGGAGGAAGAGAACGAGTATGAGGTGCAGGATCTGCGGGACCGGATCAAGTCGTCACGGGGAAGCAGATTCAATCTGATATCGAACGATTTGGGGCTGGATGGATCGTCGACGTCGACGACTCGGAGGAGAATGAGGAACTACCTGAGCCGAGAGAGCGTCATCAATGGCGTCAAAGGCCTCTCCAAGGGCATCGGCGTTATTCATCCAGACAACAGGTGGTATCGGGTATGGACGAAAGTGATACTAGTATGGGCAATCTACTCGTCCTTCTTCACACCATTCGAGTTCGGGTTTTTCAGGGGTTTGGAAGAGAGGCTCTTTATCCTAGACATCGTGGGTCAAGTAGCTTTCCTCGTCGACATTATTCTGCACTTCTTCCTCGCCTACAGGGACAGCCAGACCTATCGAATGGTCCATAAGCGCAACCTCATTGCTCTCCGGTATTTGAAATCTAGTTTCATCGTTGACTTGCTTGGTTGCATGCCTTGGGATAATATCTACAAGGCTTGTGGGAGAAGAGAGGAAGTGAGGTACCTTCTATGGATAAGGTTGAGTCGGGTGCGCAAAGTCACTAAGTTTTTCCGAGACCTTGAGAAGGATATTCGGATTAGTTATGAGTTTACCAGGATTGCAAAGCTTCTGGTTGTTGAGCTCTATTGCACACATACAGCAGCCTGCATCTTTTACTATTTGGCCACCACCTTGCCTCCTGTAGAAGAGGGGTACACATGGATTGGAAGTTTGAAGTTGGGTGACTATAGTTATTCAAACTTCAGAGATATCGATCTATGGAAGCGCTATACAACATCTTTATATTTTGCCATTGTAACCATGGCTACTGTTGGATATGGAGATATACATGCAGTAAACCTGAGGGAAATGATCTTCATAATGGCTTATGTTTCTTTTGACATGGTTCTTGGGGCTTATTTGATTGGTAACATGACAGCCTTAATTGTAAAAGGATCAAAGACAGAAAGGTTCAGGGATAAAATGACAGATCTTATCAAATATATGAACAGAAATAGACTTGGTAAGGATATCCGTAATCAGATCAAAGGCCACTTGCGTTTGCAGTATGAGAGTAGCTACACGGAGGCTGCTGTTCTCCAGGACATCCCTGCTTCAATTCGCGCTAAGATTTCACAAACCTTATATTTTCCAAACATTGTAAATGTTCCTCTCTTCAAGGGATGCTCAACGGAATTCATCAATCAGATTGTTACTAAGCTACATGAGGAATTTTTTCTTCCAGGAGAAGTGATAATGGAACCTGGAAATGTGGTAGATCAAGTTTATTTTGTCTGTCATGGTGTACTGGAGGAGGTGGGTTTAGGCGAAGATGGATCAGAAGAAACAGTTTCGCTTTTGCAACCCAACAGCTCATTTGGACAAGTCTCCATCCTTTGCAACATTCCCCAACCTTATACAGTTAGGGTATGTGAACTGTGCAGGCTTCTGCGACTGGATAAACAATCATTTACAAGTATCCTTGACATATACTTTTATGATGGGCGGAAAATCTTGAACAACCTTCTAGAGGGTAAAGGACCTCATATCAAGCAACTAGAATCAGACATTACTTTCCATATTGGAAAGCAAGAAGCTGAACTTGCTTTGAAGGTGAATAGTGCTGCTTATCATGGAGATCTGCATCAGCTAAAATGTTTGATCCGAGCTGGAGCTGATCCCAACAAGACAGATTATGATGGAAGGTCACCCTTGCATCTTGCTGCATTACGAGGGCATGAAGATATTACACTTTTCCTTATTCAGGAAGGAGTAGACATCAATATCAATGACAATTTTGGGAACACACCCTTACTTGAAGCCATCAAGAATGGTCATGATCGAGTTTCTTCTCTACTTATAAAAGAAGGGGCCTCCTTGAAGATAGAGAATGCTGGTAGTTTTTTATGTTCAGCAATAGCAAAGGGGGATTCAGATTTCCTTAAAAAGTTATTGTCAAATGGCATTGATCCGAACTCCAAGGGTTATGATCAACGGACCCCACTGCATATAGCTGCCTCAGAAGGACTATATTTGATGGCAAAGTTGCTGTTAGAAGCTGGGGCAAACGTTTTCTCAAAGGACAGGTGGGGAAACACCCCACTAGATGAAGGTCGGATGTGTGGGAACAAGAATTTGATTAGATTGCTGGAAGAAGCAAAGGATGCTCAGTTGTCGGAATTTCCATACCGTGCTCAAGAAATTGCAGATAAAATGCATCCGAAGAAATGCACAGTGTTTCCATTCCACCCATGGGATAGCAAAGAACACAGAAAACCTGGAATAGTGTTATGGGTCCCTGCCACTATTGAAAAGCTTATCGACACAGCGTCGGAGAAACTAGAATTTGAAGGTGGTATGTGTATATTATCAGAAGATGCAGGTAAAATTCTTGATGTAGGCTTGATAAATGATGGTCAGAAGCTGTATTTAGTCACCGAGACGCATTTGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

840

Amino Acids

95.8

Weight (kDa)

6.18

Isoelectric Point (pI)

39.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	93 - 343	1.5e-26	Ion transport protein
Ion_trans_2	PF07885	283 - 339	7.3e-08	Ion channel
cNMP_binding	PF00027	433 - 518	6.6e-17	Cyclic nucleotide-binding domain
Ank_2	PF12796	564 - 651	8.6e-17	Ankyrin repeats (3 copies)
Ank_4	PF13637	564 - 610	2e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	576 - 627	1e-08	Ankyrin repeats (many copies)
Ank	PF00023	589 - 621	2.3e-07	Ankyrin repeat
Ank_2	PF12796	659 - 740	3e-10	Ankyrin repeats (3 copies)
Ank_5	PF13857	673 - 725	1.7e-08	Ankyrin repeats (many copies)
Ank	PF00023	687 - 717	1.2e-06	Ankyrin repeat
KHA	PF11834	764 - 833	1.4e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1928
AatII	GACGTC	1 cut(s)	170
Acc16I	TGCGCA	1 cut(s)	620
Acc36I	ACCTGC	1 cut(s)	2438
Acc65I	GGTACC	1 cut(s)	588
AccB1I	GGYRCC	1 cut(s)	588
AccI	GTMKAC	4 cut(s)	164, 170, 414, 1842
AccII	CGCG	1 cut(s)	1191
AciI	CCGC	2 cut(s)	91, 1573
AclI	AACGTT	1 cut(s)	2136
AclWI	GGATC	8 cut(s)	93, 107, 166, 1030, 1411, 1723, 1739, 2030
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	6 cut(s)	1262, 1298, 2200, 2246, 2411, 2454
AcuI	CTGAAG	1 cut(s)	814
AcyI	GRCGYC	2 cut(s)	167, 225
AfaI	GTAC	3 cut(s)	590, 782, 1374
AfeI	AGCGCT	1 cut(s)	854
AfiI	CCNNNNNNNGG	5 cut(s)	241, 652, 1597, 1831, 2320
AhdI	GACNNNNNGTC	1 cut(s)	2395
AhlI	ACTAGT	1 cut(s)	301
AjiI	CACGTC	1 cut(s)	31
AjnI	CCWGG	5 cut(s)	680, 1167, 1309, 1330, 2344
Alw21I	GWGCWC	2 cut(s)	711, 2263
Alw26I	GTCTC	3 cut(s)	636, 1459, 2501
AlwI	GGATC	8 cut(s)	93, 107, 166, 1030, 1411, 1723, 1739, 2030
AlwNI	CAGNNNCTG	2 cut(s)	88, 2491
Aor51HI	AGCGCT	1 cut(s)	854
AoxI	GGCC	4 cut(s)	232, 750, 1117, 1938
ApeKI	GCWGC	5 cut(s)	728, 1157, 1691, 1790, 2081
ApoI	RAATTY	6 cut(s)	1262, 1298, 2200, 2246, 2411, 2454
Asp700I	GAANNNNTTC	1 cut(s)	1302
Asp718I	GGTACC	1 cut(s)	588
AspLEI	GCGC	4 cut(s)	480, 621, 855, 1193
AspS9I	GGNCC	6 cut(s)	94, 469, 1607, 1938, 2064, 2362
AsuHPI	GGTGA	4 cut(s)	821, 1693, 1767, 2494
AvaII	GGWCC	5 cut(s)	94, 469, 1607, 2064, 2362
AxyI	CCTNAGG	1 cut(s)	929
BalI	TGGCCA	1 cut(s)	752
BanI	GGYRCC	1 cut(s)	588
BanII	GRGCYC	1 cut(s)	711
Bbv12I	GWGCWC	2 cut(s)	711, 2263
BbvI	GCAGC	5 cut(s)	740, 1144, 1678, 1777, 2068
BccI	CCATC	8 cut(s)	149, 1394, 1466, 1562, 1760, 1898, 2098, 2474
BcgI	CGANNNNNNTGC	2 cut(s)	300, 334
BciT130I	CCWGG	5 cut(s)	682, 1169, 1311, 1332, 2346
BciVI	GTATCC	1 cut(s)	1556
BclI	TGATCA	1 cut(s)	2056
BcoDI	GTCTC	3 cut(s)	636, 1459, 2501
BcuI	ACTAGT	1 cut(s)	301
BfaI	CTAG	7 cut(s)	302, 383, 510, 1595, 1625, 2171, 2408
BfmI	CTRYAG	3 cut(s)	442, 768, 814
BfoI	RGCGCY	1 cut(s)	856
BfuAI	ACCTGC	1 cut(s)	2438
BfuI	GTATCC	1 cut(s)	1556
BglII	AGATCT	2 cut(s)	1057, 1705
BisI	GCNGC	5 cut(s)	729, 1158, 1692, 1791, 2082
BlsI	GCNGC	5 cut(s)	730, 1159, 1693, 1792, 2083
Bme1390I	CCNGG	5 cut(s)	682, 1169, 1311, 1332, 2346
Bme18I	GGWCC	5 cut(s)	94, 469, 1607, 2064, 2362
BmeRI	GACNNNNNGTC	1 cut(s)	2395
BmgBI	CACGTC	1 cut(s)	31
BmgT120I	GGNCC	6 cut(s)	94, 469, 1607, 1938, 2064, 2362
BmiI	GGNNCC	7 cut(s)	95, 590, 1329, 1939, 2066, 2363, 2364
BmrFI	CCNGG	5 cut(s)	682, 1169, 1311, 1332, 2346
BmsI	GCATC	8 cut(s)	255, 744, 1241, 1720, 1792, 2221, 2293, 2434
BpmI	CTGGAG	3 cut(s)	1151, 1397, 1758
Bpu10I	CCTNAGC	1 cut(s)	200
BpuEI	CTTGAG	2 cut(s)	668, 2247
Bsa29I	ATCGAT	1 cut(s)	840
BsaBI	GATNNNNATC	2 cut(s)	840, 1710
BsaHI	GRCGYC	2 cut(s)	167, 225
BsaI	GGTCTC	1 cut(s)	636
BsaJI	CCNNGG	5 cut(s)	240, 542, 888, 2046, 2318
BsaWI	WCCGGW	2 cut(s)	96, 495
BsaXI	ACNNNNNCTCC	2 cut(s)	748, 778
Bsc4I	CCNNNNNNNGG	5 cut(s)	241, 652, 1597, 1831, 2320
Bse1I	ACTGG	2 cut(s)	1380, 1527
Bse21I	CCTNAGG	1 cut(s)	929
Bse3DI	GCAATG	1 cut(s)	486
Bse8I	GATNNNNATC	2 cut(s)	840, 1710
BseBI	CCWGG	5 cut(s)	682, 1169, 1311, 1332, 2346
BseCI	ATCGAT	1 cut(s)	840
BseDI	CCNNGG	5 cut(s)	240, 542, 888, 2046, 2318
BseGI	GGATG	9 cut(s)	52, 160, 259, 1173, 1256, 1458, 2190, 2236, 2284
BseJI	GATNNNNATC	2 cut(s)	840, 1710
BseLI	CCNNNNNNNGG	5 cut(s)	241, 652, 1597, 1831, 2320
BseMI	GCAATG	1 cut(s)	486
BseMII	CTCAG	4 cut(s)	191, 920, 2100, 2249
BseNI	ACTGG	2 cut(s)	1380, 1527
BseRI	GAGGAG	4 cut(s)	22, 71, 196, 1394
BseXI	GCAGC	5 cut(s)	740, 1144, 1678, 1777, 2068
BseYI	CCCAGC	1 cut(s)	2126
BsgI	GTGCAG	3 cut(s)	101, 410, 1528
Bsh1236I	CGCG	1 cut(s)	1191
BshFI	GGCC	4 cut(s)	234, 752, 1119, 1940
BshNI	GGYRCC	1 cut(s)	588
BshVI	ATCGAT	1 cut(s)	840
BsiHKAI	GWGCWC	2 cut(s)	711, 2263
BsiSI	CCGG	2 cut(s)	97, 496
BslFI	GGGAC	3 cut(s)	107, 461, 2348
BslI	CCNNNNNNNGG	5 cut(s)	241, 652, 1597, 1831, 2320
BsmAI	GTCTC	3 cut(s)	636, 1459, 2501
BsmBI	CGTCTC	1 cut(s)	2501
BsmFI	GGGAC	3 cut(s)	107, 461, 2348
BsmI	GAATGC	1 cut(s)	1963
BsnI	GGCC	4 cut(s)	234, 752, 1119, 1940
Bso31I	GGTCTC	1 cut(s)	636
Bsp1286I	GDGCHC	2 cut(s)	711, 2263
Bsp19I	CCATGG	2 cut(s)	888, 2318
BspACI	CCGC	2 cut(s)	91, 1573
BspANI	GGCC	4 cut(s)	234, 752, 1119, 1940
BspCNI	CTCAG	4 cut(s)	192, 921, 2099, 2248
BspDI	ATCGAT	1 cut(s)	840
BspFNI	CGCG	1 cut(s)	1191
BspHI	TCATGA	1 cut(s)	1903
BspLI	GGNNCC	7 cut(s)	95, 590, 1329, 1939, 2066, 2363, 2364
BspMI	ACCTGC	1 cut(s)	2438
BspPI	GGATC	8 cut(s)	93, 107, 166, 1030, 1411, 1723, 1739, 2030
BspT107I	GGYRCC	1 cut(s)	588
BspTNI	GGTCTC	1 cut(s)	636
BsrDI	GCAATG	1 cut(s)	486
BsrI	ACTGG	2 cut(s)	1380, 1527
BssECI	CCNNGG	5 cut(s)	240, 542, 888, 2046, 2318
BssNI	GRCGYC	2 cut(s)	167, 225
BssT1I	CCWWGG	5 cut(s)	240, 542, 888, 2046, 2318
Bst2UI	CCWGG	5 cut(s)	682, 1169, 1311, 1332, 2346
Bst4CI	ACNGT	6 cut(s)	898, 1417, 1489, 1506, 2258, 2302
Bst6I	CTCTTC	5 cut(s)	57, 360, 568, 768, 1247
BstACI	GRCGYC	2 cut(s)	167, 225
BstAPI	GCANNNNNTGC	2 cut(s)	88, 2231
BstC8I	GCNNGC	3 cut(s)	539, 733, 1511
BstDEI	CTNAG	7 cut(s)	200, 630, 929, 1194, 1284, 2086, 2235
BstDSI	CCRYGG	2 cut(s)	888, 2318
BstEII	GGTNACC	1 cut(s)	1773
BstENI	CCTNNNNNAGG	4 cut(s)	239, 650, 1595, 1829
BstF5I	GGATG	9 cut(s)	52, 160, 259, 1173, 1256, 1458, 2190, 2236, 2284
BstFNI	CGCG	1 cut(s)	1191
BstH2I	RGCGCY	1 cut(s)	856
BstHHI	GCGC	4 cut(s)	480, 621, 855, 1193
BstMAI	GTCTC	3 cut(s)	636, 1459, 2501
BstMWI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1670, 2231
BstNI	CCWGG	5 cut(s)	682, 1169, 1311, 1332, 2346
BstNSI	RCATGY	2 cut(s)	541, 920
BstPI	GGTNACC	1 cut(s)	1773
BstSCI	CCNGG	5 cut(s)	680, 1167, 1309, 1330, 2344
BstSFI	CTRYAG	3 cut(s)	442, 768, 814
BstUI	CGCG	1 cut(s)	1191
BstV1I	GCAGC	5 cut(s)	740, 1144, 1678, 1777, 2068
BstX2I	RGATCY	3 cut(s)	85, 1057, 1705
BstYI	RGATCY	3 cut(s)	85, 1057, 1705
Bsu15I	ATCGAT	1 cut(s)	840
Bsu36I	CCTNAGG	1 cut(s)	929
BsuI	GTATCC	1 cut(s)	1556
BsuRI	GGCC	4 cut(s)	234, 752, 1119, 1940
BsuTUI	ATCGAT	1 cut(s)	840
BtgI	CCRYGG	2 cut(s)	888, 2318
BtrI	CACGTC	1 cut(s)	31
BtsCI	GGATG	9 cut(s)	52, 160, 259, 1173, 1256, 1458, 2190, 2236, 2284
BtsI	GCAGTG	2 cut(s)	21, 2069
BtsIMutI	CAGTG	3 cut(s)	21, 2069, 2307
BveI	ACCTGC	1 cut(s)	2438
Cac8I	GCNNGC	3 cut(s)	539, 733, 1511
CaiI	CAGNNNCTG	2 cut(s)	88, 2491
CciI	TCATGA	1 cut(s)	1903
CfoI	GCGC	4 cut(s)	480, 621, 855, 1193
Cfr13I	GGNCC	6 cut(s)	94, 469, 1607, 1938, 2064, 2362
ClaI	ATCGAT	1 cut(s)	840
CseI	GACGC	4 cut(s)	202, 214, 2385, 2518
Csp6I	GTAC	3 cut(s)	589, 781, 1373
CspCI	CAANNNNNGTGG	2 cut(s)	547, 582
CviQI	GTAC	3 cut(s)	589, 781, 1373
DdeI	CTNAG	7 cut(s)	200, 630, 929, 1194, 1284, 2086, 2235
DriI	GACNNNNNGTC	1 cut(s)	2395
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	5 cut(s)	57, 360, 568, 768, 1247
Eam1105I	GACNNNNNGTC	1 cut(s)	2395
EarI	CTCTTC	5 cut(s)	57, 360, 568, 768, 1247
EciI	GGCGGA	1 cut(s)	1588
Ecl136II	GAGCTC	1 cut(s)	709
Eco130I	CCWWGG	5 cut(s)	240, 542, 888, 2046, 2318
Eco147I	AGGCCT	1 cut(s)	234
Eco24I	GRGCYC	1 cut(s)	711
Eco31I	GGTCTC	1 cut(s)	636
Eco32I	GATATC	3 cut(s)	135, 838, 1099
Eco47I	GGWCC	5 cut(s)	94, 469, 1607, 2064, 2362
Eco47III	AGCGCT	1 cut(s)	854
Eco53kI	GAGCTC	1 cut(s)	709
Eco57I	CTGAAG	1 cut(s)	814
Eco81I	CCTNAGG	1 cut(s)	929
Eco91I	GGTNACC	1 cut(s)	1773
EcoICRI	GAGCTC	1 cut(s)	709
EcoNI	CCTNNNNNAGG	4 cut(s)	239, 650, 1595, 1829
EcoO109I	RGGNCCY	3 cut(s)	1607, 1938, 2362
EcoO65I	GGTNACC	1 cut(s)	1773
EcoRI	GAATTC	1 cut(s)	1262
EcoRII	CCWGG	5 cut(s)	680, 1167, 1309, 1330, 2344
EcoRV	GATATC	3 cut(s)	135, 838, 1099
EcoT14I	CCWWGG	5 cut(s)	240, 542, 888, 2046, 2318
EcoT22I	ATGCAT	1 cut(s)	2286
EcoT38I	GRGCYC	1 cut(s)	711
ErhI	CCWWGG	5 cut(s)	240, 542, 888, 2046, 2318
Esp3I	CGTCTC	1 cut(s)	2501
FalI	AAGNNNNNCTT	2 cut(s)	1653, 1685
FaqI	GGGAC	3 cut(s)	107, 461, 2348
FauI	CCCGC	1 cut(s)	84
FbaI	TGATCA	1 cut(s)	2056
FblI	GTMKAC	4 cut(s)	164, 170, 414, 1842
Fnu4HI	GCNGC	5 cut(s)	729, 1158, 1692, 1791, 2082
FokI	GGATG	9 cut(s)	59, 167, 246, 1160, 1263, 1445, 2197, 2243, 2271
FriOI	GRGCYC	1 cut(s)	711
Fsp4HI	GCNGC	5 cut(s)	729, 1158, 1692, 1791, 2082
FspBI	CTAG	7 cut(s)	302, 383, 510, 1595, 1625, 2171, 2408
FspI	TGCGCA	1 cut(s)	620
GlaI	GCGC	4 cut(s)	479, 620, 854, 1192
GluI	GCNGC	5 cut(s)	729, 1158, 1692, 1791, 2082
GsaI	CCCAGC	1 cut(s)	2130
GsuI	CTGGAG	3 cut(s)	1151, 1397, 1758
HaeII	RGCGCY	1 cut(s)	856
HaeIII	GGCC	4 cut(s)	234, 752, 1119, 1940
HapII	CCGG	2 cut(s)	97, 496
HgaI	GACGC	4 cut(s)	202, 214, 2385, 2518
HhaI	GCGC	4 cut(s)	480, 621, 855, 1193
Hin1I	GRCGYC	2 cut(s)	167, 225
Hin6I	GCGC	4 cut(s)	478, 619, 853, 1191
HinP1I	GCGC	4 cut(s)	478, 619, 853, 1191
HincII	GTYRAC	4 cut(s)	165, 171, 415, 523
HindII	GTYRAC	4 cut(s)	165, 171, 415, 523
HindIII	AAGCTT	2 cut(s)	692, 2381
HinfI	GANTC	5 cut(s)	123, 175, 610, 1628, 1997
HpaII	CCGG	2 cut(s)	97, 496
HphI	GGTGA	4 cut(s)	821, 1693, 1767, 2494
Hpy188III	TCNNGA	8 cut(s)	263, 1582, 1595, 1832, 1894, 1904, 2264, 2459
Hpy99I	CGWCG	6 cut(s)	166, 169, 172, 175, 416, 2401
HpyCH4III	ACNGT	6 cut(s)	898, 1417, 1489, 1506, 2258, 2302
HpyCH4IV	ACGT	3 cut(s)	30, 167, 2136
HpyF10VI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1670, 2231
HpyF3I	CTNAG	7 cut(s)	200, 630, 929, 1194, 1284, 2086, 2235
HpySE526I	ACGT	3 cut(s)	30, 167, 2136
Hsp92I	GRCGYC	2 cut(s)	167, 225
HspAI	GCGC	4 cut(s)	478, 619, 853, 1191
KflI	GGGWCCC	1 cut(s)	2362
KpnI	GGTACC	1 cut(s)	592
Ksp22I	TGATCA	1 cut(s)	2056
LmnI	GCTCC	1 cut(s)	1739
Lsp1109I	GCAGC	5 cut(s)	740, 1144, 1678, 1777, 2068
LweI	GCATC	8 cut(s)	255, 744, 1241, 1720, 1792, 2221, 2293, 2434
MaeI	CTAG	7 cut(s)	302, 383, 510, 1595, 1625, 2171, 2408
MaeII	ACGT	3 cut(s)	30, 167, 2136
MaeIII	GTNAC	8 cut(s)	108, 625, 809, 883, 995, 1279, 1773, 2500
MflI	RGATCY	3 cut(s)	85, 1057, 1705
MhlI	GDGCHC	2 cut(s)	711, 2263
MlsI	TGGCCA	1 cut(s)	752
MluNI	TGGCCA	1 cut(s)	752
MlyI	GAGTC	2 cut(s)	169, 619
MmeI	TCCRAC	4 cut(s)	880, 2162, 2223, 2379
Mox20I	TGGCCA	1 cut(s)	752
Mph1103I	ATGCAT	1 cut(s)	2286
MroXI	GAANNNNTTC	1 cut(s)	1302
MscI	TGGCCA	1 cut(s)	752
MseI	TTAA	2 cut(s)	1010, 2010
Msp20I	TGGCCA	1 cut(s)	752
MspI	CCGG	2 cut(s)	97, 496
MspR9I	CCNGG	5 cut(s)	682, 1169, 1311, 1332, 2346
Mva1269I	GAATGC	1 cut(s)	1963
MvaI	CCWGG	5 cut(s)	682, 1169, 1311, 1332, 2346
MvnI	CGCG	1 cut(s)	1191
MwoI	GCNNNNNNNGC	5 cut(s)	88, 210, 231, 1670, 2231
NcoI	CCATGG	2 cut(s)	888, 2318
NlaIV	GGNNCC	7 cut(s)	95, 590, 1329, 1939, 2066, 2363, 2364
NmeAIII	GCCGAG	1 cut(s)	230
NmuCI	GTSAC	5 cut(s)	108, 625, 809, 1773, 2500
NsbI	TGCGCA	1 cut(s)	620
NsiI	ATGCAT	1 cut(s)	2286
NspI	RCATGY	2 cut(s)	541, 920
PaeI	GCATGC	1 cut(s)	541
PagI	TCATGA	1 cut(s)	1903
PceI	AGGCCT	1 cut(s)	234
PcsI	WCGNNNNNNNCGW	2 cut(s)	167, 170
PctI	GAATGC	1 cut(s)	1963
PdmI	GAANNNNTTC	1 cut(s)	1302
PfeI	GAWTC	3 cut(s)	123, 1628, 1997
PfoI	TCCNGGA	2 cut(s)	1167, 1309
PkrI	GCNGC	5 cut(s)	730, 1159, 1693, 1792, 2083
PleI	GAGTC	2 cut(s)	169, 618
PpsI	GAGTC	2 cut(s)	169, 618
PpuMI	RGGWCCY	2 cut(s)	1607, 2362
PsiI	TTATAA	1 cut(s)	1928
Psp124BI	GAGCTC	1 cut(s)	711
Psp1406I	AACGTT	1 cut(s)	2136
Psp5II	RGGWCCY	2 cut(s)	1607, 2362
Psp6I	CCWGG	5 cut(s)	680, 1167, 1309, 1330, 2344
PspEI	GGTNACC	1 cut(s)	1773
PspFI	CCCAGC	1 cut(s)	2126
PspGI	CCWGG	5 cut(s)	680, 1167, 1309, 1330, 2344
PspN4I	GGNNCC	7 cut(s)	95, 590, 1329, 1939, 2066, 2363, 2364
PspPI	GGNCC	6 cut(s)	94, 469, 1607, 1938, 2064, 2362
PspPPI	RGGWCCY	2 cut(s)	1607, 2362
PstNI	CAGNNNCTG	2 cut(s)	88, 2491
PsuI	RGATCY	3 cut(s)	85, 1057, 1705
RsaI	GTAC	3 cut(s)	590, 782, 1374
RsaNI	GTAC	3 cut(s)	589, 781, 1373
SacI	GAGCTC	1 cut(s)	711
SalI	GTCGAC	3 cut(s)	163, 169, 413
SaqAI	TTAA	2 cut(s)	1010, 2010
SatI	GCNGC	5 cut(s)	729, 1158, 1692, 1791, 2082
Sau96I	GGNCC	6 cut(s)	94, 469, 1607, 1938, 2064, 2362
SchI	GAGTC	2 cut(s)	169, 619
ScrFI	CCNGG	5 cut(s)	682, 1169, 1311, 1332, 2346
SduI	GDGCHC	2 cut(s)	711, 2263
SfaNI	GCATC	8 cut(s)	255, 744, 1241, 1720, 1792, 2221, 2293, 2434
SfcI	CTRYAG	3 cut(s)	442, 768, 814
SgrDI	CGTCGACG	2 cut(s)	163, 169
SinI	GGWCC	5 cut(s)	94, 469, 1607, 2064, 2362
SmlI	CTYRAG	2 cut(s)	647, 2262
SmoI	CTYRAG	2 cut(s)	647, 2262
SpeI	ACTAGT	1 cut(s)	301
SphI	GCATGC	1 cut(s)	541
SseBI	AGGCCT	1 cut(s)	234
SsiI	CCGC	2 cut(s)	91, 1573
SspMI	CTAG	7 cut(s)	302, 383, 510, 1595, 1625, 2171, 2408
SstI	GAGCTC	1 cut(s)	711
StuI	AGGCCT	1 cut(s)	234
StyD4I	CCNGG	5 cut(s)	680, 1167, 1309, 1330, 2344
StyI	CCWWGG	5 cut(s)	240, 542, 888, 2046, 2318
TaaI	ACNGT	6 cut(s)	898, 1417, 1489, 1506, 2258, 2302
TaiI	ACGT	3 cut(s)	33, 170, 2139
TatI	WGTACW	1 cut(s)	1372
TfiI	GAWTC	3 cut(s)	123, 1628, 1997
Tru1I	TTAA	2 cut(s)	1010, 2010
Tru9I	TTAA	2 cut(s)	1010, 2010
TscAI	CASTG	3 cut(s)	21, 2076, 2307
TseFI	GTSAC	5 cut(s)	108, 625, 809, 1773, 2500
TseI	GCWGC	5 cut(s)	728, 1157, 1691, 1790, 2081
Tsp45I	GTSAC	5 cut(s)	108, 625, 809, 1773, 2500
TspDTI	ATGAA	7 cut(s)	248, 505, 934, 1088, 1255, 1821, 2190
TspGWI	ACGGA	4 cut(s)	1091, 1166, 1274, 2077
TspRI	CASTG	3 cut(s)	21, 2076, 2307
VpaK11BI	GGWCC	5 cut(s)	94, 469, 1607, 2064, 2362
XagI	CCTNNNNNAGG	4 cut(s)	239, 650, 1595, 1829
XapI	RAATTY	6 cut(s)	1262, 1298, 2200, 2246, 2411, 2454
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	2 cut(s)	541, 920
XcmI	CCANNNNNNNNNTGG	3 cut(s)	270, 886, 2316
XmiI	GTMKAC	4 cut(s)	164, 170, 414, 1842
XmnI	GAANNNNTTC	1 cut(s)	1302
XspI	CTAG	7 cut(s)	302, 383, 510, 1595, 1625, 2171, 2408
ZraI	GACGTC	1 cut(s)	168
Zsp2I	ATGCAT	1 cut(s)	2286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.