Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Reverse (-)

12237045 .. 12246859

Gene Structure 9815 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022790

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2520 bp

ATGTCGAGGAGAGTAGTGGAGGACGAGGACGTGGACGAGGTTGGGGATGATGAGGAGGAAGAGAACGAGTATGAGGTGCAGGATCTGCGGGACCGGATCAAGTCGTCACGGGGAAGCAGATTCAATCTGATATCGAACGATTTGGGGCTGGATGGATCTTCGACGTCGACGACTCGGAGGAGAATGAGGAACTACTTGAGCCGAGAGAGCGTCATCAATGGCGTCAAAGGCCTCTCCAAGGGCATCGGCGTTATTCATCCAGACAACAGGTGGTATCGGGTATGGACGAAAGTGATACTAGTTTGGGCAATCTACTCGTCCTTCTTCACACCATTCGAGTTCGGGTTTTTCAGGGGTTTGGAAGAGAGACTCTTTATCCTAGACATCGTGGGTCAAGTAGCTTTCCTCGTCGACATTATTCTGCACTTCTTCCTCGCCTACAGGGACAGCCAGACCTATCGAATGGTCTATAAGCGCAACCTCATTGCTCTCCGGTATTTGAAATCTAGTTTCATCGTTGACTTGCTTGGTTGCATGCCTTGGGATAATATCTACAAGGCTTGTGGGAGAAGAGAGGAAGTGAGGTACCTTCTATGGATAAGGTTGAGTCGGGTGCGCAAAGTCACTAAGTTTTTCCGAGACCTTGAGAAGGATATTCGGATTAGTTATGAGTTTACCAGGATTGCAAAGCTTCTGGTTGTTGAGCTCTATTGCACACATACAGCAGCCTGCATCTTTTACTATTTGGCCACCACCTTGCCTCCTGTAGAAGAGGGGTACACATGGATTGGAAGTTTGAAGTTGGGTGACTATAGTTATTCAAACTTCAGAGATATCGATCTATGGAAGCGCTATACAACATCTTTATATTTCGCCATTGTAACCATGGCCACTGTTGGATATGGAGATATACATGCAGTAAACCTGAGGGAAATGATCTTCATAATGGCTTATGTTTCTTTTGACATGGTTCTTGGGGCTTATTTGATTGGTAACATGACAGCCTTAATTGTAAAAGGATCAAAGACAGAAAAGTTCAGGGATAAAATGACAGATCTTATCAAATATATGAACAGAAATAGACTTGGTAAGGATATCCGTAATCAGATCAAAGGCCACTTGCGTTTGCAGTATGAGAGTAGCTACACAGAGGCTGCTGTTCTCCAGGACATCCCTGCTTCAATTCGCGCTAAGATTTCACAAACCTTATATTTTCCAAACATTGTAAATGTTCCTCTCTTCAAGGGATGCTCAACGGAATTCATCAATCAGATTGTTACTAAGCTACATGAGGAATTCTTTCTTCCAGGAGAAGTGATAATGGAACCTGGAAATGTGGTAGATCAAGTTTATTTTGTCTGTCATGGTGTACTGGAGGAGGTGGGTTTAGGCGAAGATGGATCAGAAGAAACAGTTTCGCTTTTGCAACCTAACAGCTCATTTGGACAAGTCTCCATCCTTTGCAACATTCCCCAACCTTATACAGTTAGGGTATGTGAACTGTGCAGGCTTCTGCGACTGGATAAACAATCATTTACAAGTATCCTTGACATATACTTTTATGATGGGCGGAAAATCTTGAACAACCTTCTAGAGGGTAAAGGACCTCATATCAAGCAACTAGAATCAGACATTACTTTCCATATTGGAAAGCAAGAAGCTGAACTTGCTTTGAAGGTGAATAGTGCTGCTTATCATGGAGATCTGCATCAGCTAAAATGTTTGATCCGAGCTGGAGCTGATCCCAACAAGACAGATTATGATGGAAGGTCACCCTTGCATCTTGCTGCATTACGAGGGCATGAAGATATTACACTTTTCCTTATTCAGGAAGGAGTAGACATCAACGTCAAAGACAATTTTGGGAACACACCCTTACTTGAAGCCATCAAGAATGGTCATGATCGAGTTTCTTCTCTACTTATAAAAGAAGGGGCCTCCTTGAAGATAGAGAATGCTGGTAGTTTTTTATGTTCAGCAATAGCAAAGGGGGATTCAGATTTCCTTAAAAAGTTATTGTCAAATGGCATTGATCCGAACTCCAAAGGTTATGATCAACGGACCCCACTGCATATAGCTGCCTCAGAGGGACTATATTTGATGGCAAAGTTGCTGTTAGAAGCTGGGGCAAACGTTTTCTCAAAGGACAGGTGGGGAAACACCCCACTAGATGAAGGTCGGATGTGTGGGAACAAGAATTTGATTAGATTGCTGGAAGAAGCAAAGGATGCTCAGTTGTCGGAATTCCCATACCGTGCTCAAGAAATTGCAGATAAAATGCATCCAAAGAAATGCACAGTGTTTCCATTCCACCCATGGGATGGCAAAGAACACAGAAAACCTGGAATAGTGTTATGGGTACCTGCCACTATTGAAAAGCTTATCAACACCGCGTCGGAGAAACTAGAATTTGAAGGTGGTATGTGTATATTATCAGAAGATGCAGGTAAAATTCTTGATGTAGGCTTGATAAATGATGGTCAGAAGCTGTATTTAGTCACCGAGACGCATTTGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009987 GO:0010033 GO:0010035 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033993 GO:0034220 GO:0042221 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

840

Amino Acids

95.68

Weight (kDa)

6.39

Isoelectric Point (pI)

38.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	92 - 342	2.3e-26	Ion transport protein
Ion_trans_2	PF07885	282 - 338	7.3e-08	Ion channel
cNMP_binding	PF00027	432 - 517	6.5e-17	Cyclic nucleotide-binding domain
Ank_2	PF12796	563 - 650	1e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	563 - 609	2e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	575 - 626	2.6e-09	Ankyrin repeats (many copies)
Ank	PF00023	588 - 620	9.8e-08	Ankyrin repeat
Ank_4	PF13637	599 - 641	7.3e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	658 - 739	3e-10	Ankyrin repeats (3 copies)
Ank_5	PF13857	672 - 724	1.7e-08	Ankyrin repeats (many copies)
Ank	PF00023	686 - 716	1.2e-06	Ankyrin repeat
KHA	PF11834	763 - 832	3.1e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1925
AasI	GACNNNNNNGTC	1 cut(s)	1847
AatII	GACGTC	1 cut(s)	167
Acc16I	TGCGCA	1 cut(s)	617
Acc36I	ACCTGC	2 cut(s)	2371, 2435
Acc65I	GGTACC	2 cut(s)	585, 2359
AccB1I	GGYRCC	2 cut(s)	585, 2359
AccB7I	CCANNNNNTGG	1 cut(s)	2321
AccI	GTMKAC	3 cut(s)	167, 411, 1839
AccII	CGCG	2 cut(s)	1188, 2393
AciI	CCGC	3 cut(s)	88, 1570, 2391
AclI	AACGTT	1 cut(s)	2133
AclWI	GGATC	8 cut(s)	90, 104, 163, 1027, 1408, 1720, 1736, 2027
AcoI	YGGCCR	2 cut(s)	747, 888
AcsI	RAATTY	6 cut(s)	1259, 1295, 2197, 2243, 2408, 2451
AcuI	CTGAAG	1 cut(s)	811
AcyI	GRCGYC	2 cut(s)	164, 222
AfaI	GTAC	4 cut(s)	587, 779, 1371, 2361
AfeI	AGCGCT	1 cut(s)	851
AfiI	CCNNNNNNNGG	6 cut(s)	238, 649, 1594, 1828, 2317, 2321
AhlI	ACTAGT	1 cut(s)	298
AjiI	CACGTC	1 cut(s)	31
AjnI	CCWGG	5 cut(s)	677, 1164, 1306, 1327, 2341
Alw21I	GWGCWC	2 cut(s)	708, 2260
Alw26I	GTCTC	4 cut(s)	361, 633, 1456, 2498
AlwI	GGATC	8 cut(s)	90, 104, 163, 1027, 1408, 1720, 1736, 2027
AlwNI	CAGNNNCTG	3 cut(s)	85, 1154, 2488
Aor51HI	AGCGCT	1 cut(s)	851
AoxI	GGCC	5 cut(s)	229, 747, 888, 1114, 1935
ApeKI	GCWGC	5 cut(s)	725, 1154, 1688, 1787, 2078
ApoI	RAATTY	6 cut(s)	1259, 1295, 2197, 2243, 2408, 2451
Asp700I	GAANNNNTTC	1 cut(s)	1299
Asp718I	GGTACC	2 cut(s)	585, 2359
AspLEI	GCGC	4 cut(s)	477, 618, 852, 1190
AspS9I	GGNCC	4 cut(s)	91, 1604, 1935, 2061
AsuHPI	GGTGA	4 cut(s)	818, 1690, 1764, 2491
AvaII	GGWCC	3 cut(s)	91, 1604, 2061
AxyI	CCTNAGG	1 cut(s)	926
BalI	TGGCCA	2 cut(s)	749, 890
BanI	GGYRCC	2 cut(s)	585, 2359
BanII	GRGCYC	1 cut(s)	708
Bbv12I	GWGCWC	2 cut(s)	708, 2260
BbvI	GCAGC	5 cut(s)	737, 1141, 1675, 1774, 2065
BccI	CCATC	9 cut(s)	146, 1391, 1463, 1559, 1757, 1895, 2095, 2315, 2471
BcgI	CGANNNNNNTGC	2 cut(s)	297, 331
BciT130I	CCWGG	5 cut(s)	679, 1166, 1308, 1329, 2343
BciVI	GTATCC	1 cut(s)	1553
BclI	TGATCA	1 cut(s)	2053
BcoDI	GTCTC	4 cut(s)	361, 633, 1456, 2498
BcuI	ACTAGT	1 cut(s)	298
BfaI	CTAG	7 cut(s)	299, 380, 507, 1592, 1622, 2168, 2405
BfmI	CTRYAG	3 cut(s)	439, 765, 811
BfoI	RGCGCY	1 cut(s)	853
BfuAI	ACCTGC	2 cut(s)	2371, 2435
BfuI	GTATCC	1 cut(s)	1553
BglII	AGATCT	2 cut(s)	1054, 1702
BisI	GCNGC	5 cut(s)	726, 1155, 1689, 1788, 2079
BlsI	GCNGC	5 cut(s)	727, 1156, 1690, 1789, 2080
Bme1390I	CCNGG	5 cut(s)	679, 1166, 1308, 1329, 2343
Bme18I	GGWCC	3 cut(s)	91, 1604, 2061
BmgBI	CACGTC	1 cut(s)	31
BmgT120I	GGNCC	4 cut(s)	91, 1604, 1935, 2061
BmiI	GGNNCC	6 cut(s)	92, 587, 1326, 1936, 2063, 2361
BmrFI	CCNGG	5 cut(s)	679, 1166, 1308, 1329, 2343
BmsI	GCATC	8 cut(s)	252, 741, 1238, 1717, 1789, 2218, 2290, 2431
BpmI	CTGGAG	3 cut(s)	1148, 1394, 1755
BpuEI	CTTGAG	3 cut(s)	217, 665, 2244
Bsa29I	ATCGAT	1 cut(s)	837
BsaBI	GATNNNNATC	2 cut(s)	837, 1707
BsaHI	GRCGYC	2 cut(s)	164, 222
BsaI	GGTCTC	1 cut(s)	633
BsaJI	CCNNGG	4 cut(s)	237, 539, 885, 2315
BsaWI	WCCGGW	2 cut(s)	93, 492
BsaXI	ACNNNNNCTCC	2 cut(s)	745, 775
Bsc4I	CCNNNNNNNGG	6 cut(s)	238, 649, 1594, 1828, 2317, 2321
Bse1I	ACTGG	2 cut(s)	1377, 1524
Bse21I	CCTNAGG	1 cut(s)	926
Bse3DI	GCAATG	1 cut(s)	483
Bse8I	GATNNNNATC	2 cut(s)	837, 1707
BseBI	CCWGG	5 cut(s)	679, 1166, 1308, 1329, 2343
BseCI	ATCGAT	1 cut(s)	837
BseDI	CCNNGG	4 cut(s)	237, 539, 885, 2315
BseJI	GATNNNNATC	2 cut(s)	837, 1707
BseLI	CCNNNNNNNGG	6 cut(s)	238, 649, 1594, 1828, 2317, 2321
BseMI	GCAATG	1 cut(s)	483
BseMII	CTCAG	3 cut(s)	917, 2097, 2246
BseNI	ACTGG	2 cut(s)	1377, 1524
BseRI	GAGGAG	4 cut(s)	22, 68, 193, 1391
BseXI	GCAGC	5 cut(s)	737, 1141, 1675, 1774, 2065
BseYI	CCCAGC	1 cut(s)	2123
BsgI	GTGCAG	3 cut(s)	98, 407, 1525
Bsh1236I	CGCG	2 cut(s)	1188, 2393
BshFI	GGCC	5 cut(s)	231, 749, 890, 1116, 1937
BshNI	GGYRCC	2 cut(s)	585, 2359
BshVI	ATCGAT	1 cut(s)	837
BsiHKAI	GWGCWC	2 cut(s)	708, 2260
BsiSI	CCGG	2 cut(s)	94, 493
BslFI	GGGAC	3 cut(s)	104, 458, 2103
BslI	CCNNNNNNNGG	6 cut(s)	238, 649, 1594, 1828, 2317, 2321
BsmAI	GTCTC	4 cut(s)	361, 633, 1456, 2498
BsmBI	CGTCTC	1 cut(s)	2498
BsmFI	GGGAC	3 cut(s)	104, 458, 2103
BsmI	GAATGC	1 cut(s)	1960
BsnI	GGCC	5 cut(s)	231, 749, 890, 1116, 1937
Bso31I	GGTCTC	1 cut(s)	633
Bsp1286I	GDGCHC	2 cut(s)	708, 2260
Bsp19I	CCATGG	2 cut(s)	885, 2315
BspACI	CCGC	3 cut(s)	88, 1570, 2391
BspANI	GGCC	5 cut(s)	231, 749, 890, 1116, 1937
BspCNI	CTCAG	3 cut(s)	918, 2096, 2245
BspDI	ATCGAT	1 cut(s)	837
BspFNI	CGCG	2 cut(s)	1188, 2393
BspHI	TCATGA	1 cut(s)	1900
BspLI	GGNNCC	6 cut(s)	92, 587, 1326, 1936, 2063, 2361
BspMI	ACCTGC	2 cut(s)	2371, 2435
BspPI	GGATC	8 cut(s)	90, 104, 163, 1027, 1408, 1720, 1736, 2027
BspT107I	GGYRCC	2 cut(s)	585, 2359
BspTNI	GGTCTC	1 cut(s)	633
BsrDI	GCAATG	1 cut(s)	483
BsrI	ACTGG	2 cut(s)	1377, 1524
BssECI	CCNNGG	4 cut(s)	237, 539, 885, 2315
BssNI	GRCGYC	2 cut(s)	164, 222
BssT1I	CCWWGG	4 cut(s)	237, 539, 885, 2315
Bst2UI	CCWGG	5 cut(s)	679, 1166, 1308, 1329, 2343
Bst4CI	ACNGT	6 cut(s)	895, 1414, 1486, 1503, 2255, 2299
Bst6I	CTCTTC	5 cut(s)	54, 357, 565, 765, 1244
BstACI	GRCGYC	2 cut(s)	164, 222
BstAPI	GCANNNNNTGC	2 cut(s)	85, 2228
BstC8I	GCNNGC	3 cut(s)	536, 730, 1508
BstDEI	CTNAG	6 cut(s)	627, 926, 1191, 1281, 2083, 2232
BstDSI	CCRYGG	2 cut(s)	885, 2315
BstEII	GGTNACC	1 cut(s)	1770
BstENI	CCTNNNNNAGG	4 cut(s)	236, 647, 1592, 1826
BstFNI	CGCG	2 cut(s)	1188, 2393
BstH2I	RGCGCY	1 cut(s)	853
BstHHI	GCGC	4 cut(s)	477, 618, 852, 1190
BstMAI	GTCTC	4 cut(s)	361, 633, 1456, 2498
BstMWI	GCNNNNNNNGC	5 cut(s)	85, 207, 228, 1667, 2228
BstNI	CCWGG	5 cut(s)	679, 1166, 1308, 1329, 2343
BstNSI	RCATGY	2 cut(s)	538, 917
BstPI	GGTNACC	1 cut(s)	1770
BstSCI	CCNGG	5 cut(s)	677, 1164, 1306, 1327, 2341
BstSFI	CTRYAG	3 cut(s)	439, 765, 811
BstUI	CGCG	2 cut(s)	1188, 2393
BstV1I	GCAGC	5 cut(s)	737, 1141, 1675, 1774, 2065
BstX2I	RGATCY	4 cut(s)	82, 155, 1054, 1702
BstYI	RGATCY	4 cut(s)	82, 155, 1054, 1702
Bsu15I	ATCGAT	1 cut(s)	837
Bsu36I	CCTNAGG	1 cut(s)	926
BsuI	GTATCC	1 cut(s)	1553
BsuRI	GGCC	5 cut(s)	231, 749, 890, 1116, 1937
BsuTUI	ATCGAT	1 cut(s)	837
BtgI	CCRYGG	2 cut(s)	885, 2315
BtrI	CACGTC	1 cut(s)	31
BtsI	GCAGTG	1 cut(s)	2066
BtsIMutI	CAGTG	3 cut(s)	891, 2066, 2304
BveI	ACCTGC	2 cut(s)	2371, 2435
Cac8I	GCNNGC	3 cut(s)	536, 730, 1508
CaiI	CAGNNNCTG	3 cut(s)	85, 1154, 2488
CciI	TCATGA	1 cut(s)	1900
CfoI	GCGC	4 cut(s)	477, 618, 852, 1190
Cfr13I	GGNCC	4 cut(s)	91, 1604, 1935, 2061
ClaI	ATCGAT	1 cut(s)	837
CseI	GACGC	4 cut(s)	199, 211, 2382, 2515
Csp6I	GTAC	4 cut(s)	586, 778, 1370, 2360
CspCI	CAANNNNNGTGG	2 cut(s)	544, 579
CviQI	GTAC	4 cut(s)	586, 778, 1370, 2360
DdeI	CTNAG	6 cut(s)	627, 926, 1191, 1281, 2083, 2232
DrdI	GACNNNNNNGTC	1 cut(s)	1847
DseDI	GACNNNNNNGTC	1 cut(s)	1847
EaeI	YGGCCR	2 cut(s)	747, 888
Eam1104I	CTCTTC	5 cut(s)	54, 357, 565, 765, 1244
EarI	CTCTTC	5 cut(s)	54, 357, 565, 765, 1244
EciI	GGCGGA	1 cut(s)	1585
Ecl136II	GAGCTC	1 cut(s)	706
Eco130I	CCWWGG	4 cut(s)	237, 539, 885, 2315
Eco147I	AGGCCT	1 cut(s)	231
Eco24I	GRGCYC	1 cut(s)	708
Eco31I	GGTCTC	1 cut(s)	633
Eco32I	GATATC	3 cut(s)	132, 835, 1096
Eco47I	GGWCC	3 cut(s)	91, 1604, 2061
Eco47III	AGCGCT	1 cut(s)	851
Eco53kI	GAGCTC	1 cut(s)	706
Eco57I	CTGAAG	1 cut(s)	811
Eco81I	CCTNAGG	1 cut(s)	926
Eco91I	GGTNACC	1 cut(s)	1770
EcoICRI	GAGCTC	1 cut(s)	706
EcoNI	CCTNNNNNAGG	4 cut(s)	236, 647, 1592, 1826
EcoO109I	RGGNCCY	2 cut(s)	1604, 1935
EcoO65I	GGTNACC	1 cut(s)	1770
EcoRI	GAATTC	3 cut(s)	1259, 1295, 2243
EcoRII	CCWGG	5 cut(s)	677, 1164, 1306, 1327, 2341
EcoRV	GATATC	3 cut(s)	132, 835, 1096
EcoT14I	CCWWGG	4 cut(s)	237, 539, 885, 2315
EcoT22I	ATGCAT	1 cut(s)	2283
EcoT38I	GRGCYC	1 cut(s)	708
ErhI	CCWWGG	4 cut(s)	237, 539, 885, 2315
Esp3I	CGTCTC	1 cut(s)	2498
FalI	AAGNNNNNCTT	2 cut(s)	1650, 1682
FaqI	GGGAC	3 cut(s)	104, 458, 2103
FauI	CCCGC	1 cut(s)	81
FbaI	TGATCA	1 cut(s)	2053
FblI	GTMKAC	3 cut(s)	167, 411, 1839
Fnu4HI	GCNGC	5 cut(s)	726, 1155, 1689, 1788, 2079
FriOI	GRGCYC	1 cut(s)	708
Fsp4HI	GCNGC	5 cut(s)	726, 1155, 1689, 1788, 2079
FspBI	CTAG	7 cut(s)	299, 380, 507, 1592, 1622, 2168, 2405
FspI	TGCGCA	1 cut(s)	617
GlaI	GCGC	4 cut(s)	476, 617, 851, 1189
GluI	GCNGC	5 cut(s)	726, 1155, 1689, 1788, 2079
GsaI	CCCAGC	1 cut(s)	2127
GsuI	CTGGAG	3 cut(s)	1148, 1394, 1755
HaeII	RGCGCY	1 cut(s)	853
HaeIII	GGCC	5 cut(s)	231, 749, 890, 1116, 1937
HapII	CCGG	2 cut(s)	94, 493
HgaI	GACGC	4 cut(s)	199, 211, 2382, 2515
HhaI	GCGC	4 cut(s)	477, 618, 852, 1190
Hin1I	GRCGYC	2 cut(s)	164, 222
Hin6I	GCGC	4 cut(s)	475, 616, 850, 1188
HinP1I	GCGC	4 cut(s)	475, 616, 850, 1188
HincII	GTYRAC	3 cut(s)	168, 412, 520
HindII	GTYRAC	3 cut(s)	168, 412, 520
HindIII	AAGCTT	2 cut(s)	689, 2378
HinfI	GANTC	6 cut(s)	120, 172, 369, 607, 1625, 1994
HpaII	CCGG	2 cut(s)	94, 493
HphI	GGTGA	4 cut(s)	818, 1690, 1764, 2491
Hpy188III	TCNNGA	8 cut(s)	260, 1579, 1592, 1829, 1891, 1901, 2261, 2456
Hpy99I	CGWCG	5 cut(s)	166, 169, 172, 413, 2398
HpyCH4III	ACNGT	6 cut(s)	895, 1414, 1486, 1503, 2255, 2299
HpyCH4IV	ACGT	4 cut(s)	30, 164, 1848, 2133
HpyF10VI	GCNNNNNNNGC	5 cut(s)	85, 207, 228, 1667, 2228
HpyF3I	CTNAG	6 cut(s)	627, 926, 1191, 1281, 2083, 2232
HpySE526I	ACGT	4 cut(s)	30, 164, 1848, 2133
Hsp92I	GRCGYC	2 cut(s)	164, 222
HspAI	GCGC	4 cut(s)	475, 616, 850, 1188
KpnI	GGTACC	2 cut(s)	589, 2363
Ksp22I	TGATCA	1 cut(s)	2053
LmnI	GCTCC	1 cut(s)	1736
Lsp1109I	GCAGC	5 cut(s)	737, 1141, 1675, 1774, 2065
LweI	GCATC	8 cut(s)	252, 741, 1238, 1717, 1789, 2218, 2290, 2431
MaeI	CTAG	7 cut(s)	299, 380, 507, 1592, 1622, 2168, 2405
MaeII	ACGT	4 cut(s)	30, 164, 1848, 2133
MaeIII	GTNAC	8 cut(s)	105, 622, 806, 880, 992, 1276, 1770, 2497
MflI	RGATCY	4 cut(s)	82, 155, 1054, 1702
MhlI	GDGCHC	2 cut(s)	708, 2260
MlsI	TGGCCA	2 cut(s)	749, 890
MluNI	TGGCCA	2 cut(s)	749, 890
MlyI	GAGTC	3 cut(s)	166, 363, 616
MmeI	TCCRAC	4 cut(s)	877, 2159, 2220, 2376
Mox20I	TGGCCA	2 cut(s)	749, 890
Mph1103I	ATGCAT	1 cut(s)	2283
MroXI	GAANNNNTTC	1 cut(s)	1299
MscI	TGGCCA	2 cut(s)	749, 890
MseI	TTAA	2 cut(s)	1007, 2007
Msp20I	TGGCCA	2 cut(s)	749, 890
MspI	CCGG	2 cut(s)	94, 493
MspR9I	CCNGG	5 cut(s)	679, 1166, 1308, 1329, 2343
Mva1269I	GAATGC	1 cut(s)	1960
MvaI	CCWGG	5 cut(s)	679, 1166, 1308, 1329, 2343
MvnI	CGCG	2 cut(s)	1188, 2393
MwoI	GCNNNNNNNGC	5 cut(s)	85, 207, 228, 1667, 2228
NcoI	CCATGG	2 cut(s)	885, 2315
NlaIV	GGNNCC	6 cut(s)	92, 587, 1326, 1936, 2063, 2361
NmeAIII	GCCGAG	1 cut(s)	227
NmuCI	GTSAC	5 cut(s)	105, 622, 806, 1770, 2497
NsbI	TGCGCA	1 cut(s)	617
NsiI	ATGCAT	1 cut(s)	2283
NspI	RCATGY	2 cut(s)	538, 917
PaeI	GCATGC	1 cut(s)	538
PagI	TCATGA	1 cut(s)	1900
PceI	AGGCCT	1 cut(s)	231
PcsI	WCGNNNNNNNCGW	1 cut(s)	167
PctI	GAATGC	1 cut(s)	1960
PdmI	GAANNNNTTC	1 cut(s)	1299
PfeI	GAWTC	3 cut(s)	120, 1625, 1994
PflMI	CCANNNNNTGG	1 cut(s)	2321
PfoI	TCCNGGA	2 cut(s)	1164, 1306
PkrI	GCNGC	5 cut(s)	727, 1156, 1690, 1789, 2080
PleI	GAGTC	3 cut(s)	166, 363, 615
PpsI	GAGTC	3 cut(s)	166, 363, 615
PpuMI	RGGWCCY	1 cut(s)	1604
PsiI	TTATAA	1 cut(s)	1925
Psp124BI	GAGCTC	1 cut(s)	708
Psp1406I	AACGTT	1 cut(s)	2133
Psp5II	RGGWCCY	1 cut(s)	1604
Psp6I	CCWGG	5 cut(s)	677, 1164, 1306, 1327, 2341
PspEI	GGTNACC	1 cut(s)	1770
PspFI	CCCAGC	1 cut(s)	2123
PspGI	CCWGG	5 cut(s)	677, 1164, 1306, 1327, 2341
PspN4I	GGNNCC	6 cut(s)	92, 587, 1326, 1936, 2063, 2361
PspPI	GGNCC	4 cut(s)	91, 1604, 1935, 2061
PspPPI	RGGWCCY	1 cut(s)	1604
PstNI	CAGNNNCTG	3 cut(s)	85, 1154, 2488
PsuI	RGATCY	4 cut(s)	82, 155, 1054, 1702
RsaI	GTAC	4 cut(s)	587, 779, 1371, 2361
RsaNI	GTAC	4 cut(s)	586, 778, 1370, 2360
SacI	GAGCTC	1 cut(s)	708
SalI	GTCGAC	2 cut(s)	166, 410
SaqAI	TTAA	2 cut(s)	1007, 2007
SatI	GCNGC	5 cut(s)	726, 1155, 1689, 1788, 2079
Sau96I	GGNCC	4 cut(s)	91, 1604, 1935, 2061
SchI	GAGTC	3 cut(s)	166, 363, 616
ScrFI	CCNGG	5 cut(s)	679, 1166, 1308, 1329, 2343
SduI	GDGCHC	2 cut(s)	708, 2260
SfaNI	GCATC	8 cut(s)	252, 741, 1238, 1717, 1789, 2218, 2290, 2431
SfcI	CTRYAG	3 cut(s)	439, 765, 811
SgrDI	CGTCGACG	1 cut(s)	166
SinI	GGWCC	3 cut(s)	91, 1604, 2061
SmlI	CTYRAG	3 cut(s)	196, 644, 2259
SmoI	CTYRAG	3 cut(s)	196, 644, 2259
SpeI	ACTAGT	1 cut(s)	298
SphI	GCATGC	1 cut(s)	538
SseBI	AGGCCT	1 cut(s)	231
SsiI	CCGC	3 cut(s)	88, 1570, 2391
SspMI	CTAG	7 cut(s)	299, 380, 507, 1592, 1622, 2168, 2405
SstI	GAGCTC	1 cut(s)	708
StuI	AGGCCT	1 cut(s)	231
StyD4I	CCNGG	5 cut(s)	677, 1164, 1306, 1327, 2341
StyI	CCWWGG	4 cut(s)	237, 539, 885, 2315
TaaI	ACNGT	6 cut(s)	895, 1414, 1486, 1503, 2255, 2299
TaiI	ACGT	4 cut(s)	33, 167, 1851, 2136
TaqI	TCGA	9 cut(s)	5, 134, 161, 167, 336, 411, 460, 837, 1906
TatI	WGTACW	1 cut(s)	1369
TfiI	GAWTC	3 cut(s)	120, 1625, 1994
Tru1I	TTAA	2 cut(s)	1007, 2007
Tru9I	TTAA	2 cut(s)	1007, 2007
TscAI	CASTG	3 cut(s)	898, 2073, 2304
TseFI	GTSAC	5 cut(s)	105, 622, 806, 1770, 2497
TseI	GCWGC	5 cut(s)	725, 1154, 1688, 1787, 2078
Tsp45I	GTSAC	5 cut(s)	105, 622, 806, 1770, 2497
TspDTI	ATGAA	7 cut(s)	245, 502, 931, 1085, 1252, 1818, 2187
TspGWI	ACGGA	3 cut(s)	1088, 1271, 2074
TspRI	CASTG	3 cut(s)	898, 2073, 2304
Van91I	CCANNNNNTGG	1 cut(s)	2321
VpaK11BI	GGWCC	3 cut(s)	91, 1604, 2061
XagI	CCTNNNNNAGG	4 cut(s)	236, 647, 1592, 1826
XapI	RAATTY	6 cut(s)	1259, 1295, 2197, 2243, 2408, 2451
XbaI	TCTAGA	1 cut(s)	1591
XceI	RCATGY	2 cut(s)	538, 917
XcmI	CCANNNNNNNNNTGG	4 cut(s)	267, 883, 2313, 2318
XmiI	GTMKAC	3 cut(s)	167, 411, 1839
XmnI	GAANNNNTTC	1 cut(s)	1299
XspI	CTAG	7 cut(s)	299, 380, 507, 1592, 1622, 2168, 2405
ZraI	GACGTC	1 cut(s)	165
Zsp2I	ATGCAT	1 cut(s)	2283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.