Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Forward (+)

68038840 .. 68044624

Gene Structure 5785 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005488

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGCATGGAGCTCAAGAAAGACGCAGATCGTCAGGAGGAGCTGAGGGTCACCGCCACGACTCCTCTGACGATGAAGGATACGAGGTCGAGGACGACGAGCTCATCACCGATTCGAGGTCGTCGTCGAGACAGTTCTGGTGGAAAAGGCGTTTCTCTTTGAAGAGAAGTGAGTCTGGCACTAATGCCACCACTGTTGAGACTACTGCGGCCAATGCAAGCAGAACATCATCATCATCGGCTTGTAATTCGCTTTTCAGTAGAATGGTCATTCATCCCGATAACTGGTGGTACGTGGGGTGGACGCATTTCATTCTGTTATGGGCAGTCTACTCCTCCTTCTTCACACCTCTGGAGTTCGGCTTCTTCAGAGGTCTGCCGGAGAACCTGTTCATGCTCGACAGCTGCGGCCAGATAGCCTTTCTCATCGATATCATCGTCCGCTTCTTCGTCGCCTATCGCGACTTCCACTCCCACCGCATTGTCTACGATCGCCGCCTCATCGCTCTCCGATACCTCAAGTCTCGTTTCCTCGTCGATTTTCTCGGCTGTCTTCCCTGGGATGCCATCTACAAGGCTTCTGGGAGAGAAGAGGCGGTGAGGTACTTATTGTGGATACGGCTAAGCCGGGCACGGAGAGTAACCGAGTTTTTTGAGAAATTGGAGAAAGATATCAGAATCAATTACCTGTTTACAAGAATTGCCAAGCTATTTGTTGTTGAACTTTATAGCACACACACAGCTGCTTGCATATTTTATTATCTGGCTACTACCATGCCTCCATCACAGGAAGGTTACACATGGATTGGGAGTTTGAAGATGGGTGACTATAGCTATGCACATTTCAGGGAGATTGATCTTTGGAAGCGTTACATAACTTCGCTCTATTTTGCAGTTGTTACCATGGCCACCGTTGGTTATGGAGAGATACATGCAGTGAATGTGAGGGAGATGATATTCATCATGTTTTATGTGTCTTTTGATATGATTCTTGGTGCTTATCTGCTTGGTAACATTGCGGCATTGATAGTGAAAGGATCAAAAACAGAGAAGTTTAGGGACAAGATGGCGGACCTTATCAAATACATGAATAGAAACCGTATTGATAGAGGGATAAGTAAGGAGATTAAAAACCATTTGAGATTACAGTATGACCGTAGCTACACCGAAGCTGCTGTTCTTCAGGATATTCCTGCCTCTATTCGCAGTAAGATTTCACAGAAATTGTATGAGCCATACATCAAAGAAGTTCATCTTTTTAAAGGGTGCTCTACAGGATTCATAAAACAGATAGCAACCAGAATTCATGAGGAGTTTTTCCTTCCAGCAGAAGTGATTATAGAGCAGGGGGATGTAGTAGATCACCTATATATAGTATGCCATGGTGAACTGGAAAAGCTGGGAAGGAGTGAAGATGATGACAATGACGAGCTCCTTGAGCGTTTGCAAACCTATAGCTCATTTGGAGAGGTTTCTTTTCTTTGCAACACTCCCCAGCCGTATGCTGTTCGGGTTTGTCAACTGTGTAGGGTCTTACGACTTGATAGACAGTCCTTTAGAGAAATACTGGAGATATATTTCTTAGATGGCCGGCTTATCTTGGATAACCTGCTTGAGGGAAAAGACTTGTATACCCGGAATAGGCTTTTGGAATCAGACATCACTTTATATATCGGAAAGCATGAAATGGAGTTAGTTATGAAAGTAAATTGTGCTGCCTATGATGGAGATTTTTATCGAGTAAAACGTTTGATTGGAGCTGGTGTAGATCCAAACAAGGTGGATTATGCTGGAAGGTCCCCTTTGCATGCTGCTGCATCAAAAGGGTACGAGGATATTGCTCGTTTTCTTATCGAAAAAGGGGGAGACGTCAAGATGTCTGATAAGTTTGGGAACACTCCATTGCTTGAAGCCATAAAAAATGGCCATGATCAAGTTGCTTCTTTACTTGTTCAGGCAGGGGCATCACTTACTATTGAAGATGCTGGTGATTTTCTCTGTGCTACTGTAGCAAAGAGGAATTTGGACCTCGTGAAAAGGCTTTTAGCCAATGACATGAACCCAAATGCGAAAAATTATGATCAGAGATCACCACTACATGTAGCTGCTTCAGAAGGGTTATATCCAATGGCAGAGTTCCTAGTAGATGCAGGAGCAAGTGTTCTATCAAAGGACAGGTACAATCTGACTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

731

Amino Acids

83.95

Weight (kDa)

6.7

Isoelectric Point (pI)

45.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	100 - 347	1.1e-25	Ion transport protein
Ion_trans_2	PF07885	287 - 343	3.9e-08	Ion channel
cNMP_binding	PF00027	438 - 523	1.6e-16	Cyclic nucleotide-binding domain
Ank_4	PF13637	570 - 617	3.3e-10	Ankyrin repeats (many copies)
Ank_2	PF12796	571 - 660	7.2e-17	Ankyrin repeats (3 copies)
Ank_5	PF13857	587 - 634	1.5e-07	Ankyrin repeats (many copies)
Ank	PF00023	597 - 627	6.5e-06	Ankyrin repeat
Ank_4	PF13637	601 - 649	1.4e-08	Ankyrin repeats (many copies)
Ank_2	PF12796	666 - 724	4.5e-07	Ankyrin repeats (3 copies)
Ank_4	PF13637	670 - 714	2.7e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	680 - 729	2.5e-06	Ankyrin repeats (many copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1869
Acc36I	ACCTGC	1 cut(s)	1614
AccI	GTMKAC	3 cut(s)	327, 483, 1628
AccII	CGCG	1 cut(s)	459
AciI	CCGC	9 cut(s)	52, 206, 405, 439, 475, 493, 593, 1016, 1067
AclI	AACGTT	1 cut(s)	1744
AclWI	GGATC	2 cut(s)	1042, 1760
AcoI	YGGCCR	5 cut(s)	207, 406, 903, 1585, 1921
AcsI	RAATTY	2 cut(s)	1299, 2017
AcuI	CTGAAG	3 cut(s)	349, 1163, 2091
AcyI	GRCGYC	1 cut(s)	1866
AfaI	GTAC	4 cut(s)	290, 602, 1826, 2177
AfiI	CCNNNNNNNGG	4 cut(s)	114, 282, 1612, 1638
AflIII	ACRYGT	1 cut(s)	2095
AgsI	TTSAA	5 cut(s)	160, 719, 814, 1907, 1976
AjnI	CCWGG	1 cut(s)	554
AjuI	GAANNNNNNNTTGG	2 cut(s)	1628, 1660
AloI	GAACNNNNNNTCC	4 cut(s)	371, 403, 2142, 2174
Alw21I	GWGCWC	4 cut(s)	13, 102, 1268, 1431
Alw26I	GTCTC	4 cut(s)	121, 191, 525, 1857
AlwI	GGATC	2 cut(s)	1042, 1760
AoxI	GGCC	5 cut(s)	207, 406, 903, 1585, 1921
ApeKI	GCWGC	7 cut(s)	402, 740, 1169, 1712, 1808, 1811, 2102
ApoI	RAATTY	2 cut(s)	1299, 2017
Asp700I	GAANNNNTTC	1 cut(s)	386
AspS9I	GGNCC	3 cut(s)	1069, 1794, 2023
AsuC2I	CCSGG	2 cut(s)	626, 1633
AsuHPI	GGTGA	8 cut(s)	41, 97, 607, 833, 1352, 1394, 1997, 2079
AvaII	GGWCC	3 cut(s)	1069, 1794, 2023
BaeGI	GKGCMC	1 cut(s)	631
BalI	TGGCCA	2 cut(s)	905, 1923
BanII	GRGCYC	3 cut(s)	13, 102, 1431
BauI	CACGAG	1 cut(s)	2027
BbsI	GAAGAC	1 cut(s)	542
Bbv12I	GWGCWC	4 cut(s)	13, 102, 1268, 1431
BbvCI	CCTCAGC	1 cut(s)	42
BbvI	GCAGC	7 cut(s)	389, 727, 1156, 1699, 1795, 1798, 2089
BccI	CCATC	6 cut(s)	572, 787, 811, 1057, 1577, 1715
BceAI	ACGGC	2 cut(s)	632, 1480
BcgI	CGANNNNNNTGC	2 cut(s)	1817, 1851
BciT130I	CCWGG	1 cut(s)	556
BciVI	GTATCC	2 cut(s)	71, 606
BclI	TGATCA	2 cut(s)	1927, 2077
BcnI	CCSGG	2 cut(s)	626, 1633
BcoDI	GTCTC	4 cut(s)	121, 191, 525, 1857
BfaI	CTAG	1 cut(s)	2138
BfmI	CTRYAG	4 cut(s)	826, 1269, 1450, 2004
BfuAI	ACCTGC	1 cut(s)	1614
BfuI	GTATCC	2 cut(s)	71, 606
BlpI	GCTNAGC	1 cut(s)	620
Bme1390I	CCNGG	3 cut(s)	556, 626, 1633
Bme18I	GGWCC	3 cut(s)	1069, 1794, 2023
BmgT120I	GGNCC	3 cut(s)	1069, 1794, 2023
BmiI	GGNNCC	1 cut(s)	1796
BmrFI	CCNGG	3 cut(s)	556, 626, 1633
BmsI	GCATC	5 cut(s)	550, 1823, 1969, 1970, 2134
BpiI	GAAGAC	1 cut(s)	542
BpmI	CTGGAG	2 cut(s)	371, 1586
Bpu10I	CCTNAGC	1 cut(s)	42
Bpu1102I	GCTNAGC	1 cut(s)	620
BpuEI	CTTGAG	3 cut(s)	500, 1454, 1631
BpuMI	CCSGG	2 cut(s)	626, 1633
Bsa29I	ATCGAT	1 cut(s)	426
BsaAI	YACGTR	1 cut(s)	292
BsaBI	GATNNNNATC	2 cut(s)	956, 1731
BsaHI	GRCGYC	1 cut(s)	1866
BsaJI	CCNNGG	4 cut(s)	554, 555, 900, 1378
Bsc4I	CCNNNNNNNGG	4 cut(s)	114, 282, 1612, 1638
Bse118I	RCCGGY	1 cut(s)	1587
Bse1I	ACTGG	3 cut(s)	287, 1392, 1569
Bse3DI	GCAATG	2 cut(s)	1011, 1898
Bse8I	GATNNNNATC	2 cut(s)	956, 1731
BseBI	CCWGG	1 cut(s)	556
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	4 cut(s)	554, 555, 900, 1378
BseGI	GGATG	3 cut(s)	271, 565, 1354
BseJI	GATNNNNATC	2 cut(s)	956, 1731
BseLI	CCNNNNNNNGG	4 cut(s)	114, 282, 1612, 1638
BseMI	GCAATG	2 cut(s)	1011, 1898
BseMII	CTCAG	1 cut(s)	33
BseNI	ACTGG	3 cut(s)	287, 1392, 1569
BseRI	GAGGAG	4 cut(s)	51, 52, 322, 1322
BseSI	GKGCMC	1 cut(s)	631
BseXI	GCAGC	7 cut(s)	389, 727, 1156, 1699, 1795, 1798, 2089
BseYI	CCCAGC	2 cut(s)	1396, 1491
Bsh1236I	CGCG	1 cut(s)	459
Bsh1285I	CGRYCG	1 cut(s)	490
BshFI	GGCC	5 cut(s)	209, 408, 905, 1587, 1923
BshVI	ATCGAT	1 cut(s)	426
BsiEI	CGRYCG	1 cut(s)	490
BsiHKAI	GWGCWC	4 cut(s)	13, 102, 1268, 1431
BsiSI	CCGG	4 cut(s)	377, 625, 1588, 1633
BslFI	GGGAC	2 cut(s)	1070, 1780
BslI	CCNNNNNNNGG	4 cut(s)	114, 282, 1612, 1638
BsmAI	GTCTC	4 cut(s)	121, 191, 525, 1857
BsmBI	CGTCTC	1 cut(s)	1857
BsmFI	GGGAC	2 cut(s)	1070, 1780
BsnI	GGCC	5 cut(s)	209, 408, 905, 1587, 1923
Bsp1286I	GDGCHC	5 cut(s)	13, 102, 631, 1268, 1431
Bsp143I	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
Bsp1720I	GCTNAGC	1 cut(s)	620
Bsp19I	CCATGG	2 cut(s)	900, 1378
Bsp68I	TCGCGA	1 cut(s)	459
BspACI	CCGC	9 cut(s)	52, 206, 405, 439, 475, 493, 593, 1016, 1067
BspANI	GGCC	5 cut(s)	209, 408, 905, 1587, 1923
BspCNI	CTCAG	1 cut(s)	34
BspDI	ATCGAT	1 cut(s)	426
BspFNI	CGCG	1 cut(s)	459
BspHI	TCATGA	1 cut(s)	1303
BspLI	GGNNCC	1 cut(s)	1796
BspMI	ACCTGC	1 cut(s)	1614
BspPI	GGATC	2 cut(s)	1042, 1760
BsrDI	GCAATG	2 cut(s)	1011, 1898
BsrFI	RCCGGY	1 cut(s)	1587
BsrI	ACTGG	3 cut(s)	287, 1392, 1569
BssAI	RCCGGY	1 cut(s)	1587
BssECI	CCNNGG	4 cut(s)	554, 555, 900, 1378
BssMI	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
BssNAI	GTATAC	1 cut(s)	1629
BssNI	GRCGYC	1 cut(s)	1866
BssSI	CACGAG	1 cut(s)	2027
BssT1I	CCWWGG	2 cut(s)	900, 1378
Bst1107I	GTATAC	1 cut(s)	1629
Bst2BI	CACGAG	1 cut(s)	2027
Bst2UI	CCWGG	1 cut(s)	556
Bst4CI	ACNGT	9 cut(s)	132, 193, 910, 1097, 1146, 1154, 1521, 1548, 2005
Bst6I	CTCTTC	2 cut(s)	155, 582
BstACI	GRCGYC	1 cut(s)	1866
BstBAI	YACGTR	1 cut(s)	292
BstC8I	GCNNGC	4 cut(s)	217, 745, 1589, 1806
BstDEI	CTNAG	3 cut(s)	42, 620, 1579
BstDSI	CCRYGG	2 cut(s)	900, 1378
BstEII	GGTNACC	1 cut(s)	47
BstENI	CCTNNNNNAGG	1 cut(s)	1610
BstF5I	GGATG	3 cut(s)	271, 565, 1354
BstFNI	CGCG	1 cut(s)	459
BstKTI	GATC	9 cut(s)	29, 490, 856, 1037, 1360, 1768, 1930, 2080, 2087
BstMAI	GTCTC	4 cut(s)	121, 191, 525, 1857
BstMBI	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
BstMCI	CGRYCG	1 cut(s)	490
BstMWI	GCNNNNNNNGC	3 cut(s)	212, 1435, 2006
BstNI	CCWGG	1 cut(s)	556
BstNSI	RCATGY	3 cut(s)	932, 1808, 2099
BstPI	GGTNACC	1 cut(s)	47
BstSCI	CCNGG	3 cut(s)	554, 624, 1631
BstSFI	CTRYAG	4 cut(s)	826, 1269, 1450, 2004
BstSLI	GKGCMC	1 cut(s)	631
BstUI	CGCG	1 cut(s)	459
BstV1I	GCAGC	7 cut(s)	389, 727, 1156, 1699, 1795, 1798, 2089
BstV2I	GAAGAC	1 cut(s)	542
BstX2I	RGATCY	1 cut(s)	1765
BstYI	RGATCY	1 cut(s)	1765
BstZ17I	GTATAC	1 cut(s)	1629
Bsu15I	ATCGAT	1 cut(s)	426
BsuI	GTATCC	2 cut(s)	71, 606
BsuRI	GGCC	5 cut(s)	209, 408, 905, 1587, 1923
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	2 cut(s)	900, 1378
BtgZI	GCGATG	1 cut(s)	484
BtsCI	GGATG	3 cut(s)	271, 565, 1354
BtsI	GCAGTG	1 cut(s)	939
BtsIMutI	CAGTG	2 cut(s)	189, 939
BtuMI	TCGCGA	1 cut(s)	459
BveI	ACCTGC	1 cut(s)	1614
Cac8I	GCNNGC	4 cut(s)	217, 745, 1589, 1806
CciI	TCATGA	1 cut(s)	1303
Cfr10I	RCCGGY	1 cut(s)	1587
Cfr13I	GGNCC	3 cut(s)	1069, 1794, 2023
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	2 cut(s)	30, 310
Csp6I	GTAC	4 cut(s)	289, 601, 1825, 2176
CspCI	CAANNNNNGTGG	4 cut(s)	175, 210, 1758, 1793
CviQI	GTAC	4 cut(s)	289, 601, 1825, 2176
DdeI	CTNAG	3 cut(s)	42, 620, 1579
DpnI	GATC	9 cut(s)	28, 489, 855, 1036, 1359, 1767, 1929, 2079, 2086
DpnII	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
DraI	TTTAAA	1 cut(s)	1258
EaeI	YGGCCR	5 cut(s)	207, 406, 903, 1585, 1921
Eam1104I	CTCTTC	2 cut(s)	155, 582
EarI	CTCTTC	2 cut(s)	155, 582
EciI	GGCGGA	1 cut(s)	1082
Ecl136II	GAGCTC	3 cut(s)	11, 100, 1429
Eco130I	CCWWGG	2 cut(s)	900, 1378
Eco24I	GRGCYC	3 cut(s)	13, 102, 1431
Eco32I	GATATC	2 cut(s)	430, 670
Eco47I	GGWCC	3 cut(s)	1069, 1794, 2023
Eco53kI	GAGCTC	3 cut(s)	11, 100, 1429
Eco57I	CTGAAG	3 cut(s)	349, 1163, 2091
Eco91I	GGTNACC	1 cut(s)	47
EcoICRI	GAGCTC	3 cut(s)	11, 100, 1429
EcoNI	CCTNNNNNAGG	1 cut(s)	1610
EcoO109I	RGGNCCY	1 cut(s)	1794
EcoO65I	GGTNACC	1 cut(s)	47
EcoRI	GAATTC	1 cut(s)	1299
EcoRII	CCWGG	1 cut(s)	554
EcoRV	GATATC	2 cut(s)	430, 670
EcoT14I	CCWWGG	2 cut(s)	900, 1378
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	13, 102, 1431
ErhI	CCWWGG	2 cut(s)	900, 1378
Esp3I	CGTCTC	1 cut(s)	1857
FalI	AAGNNNNNCTT	4 cut(s)	1236, 1268, 1783, 1815
FaqI	GGGAC	2 cut(s)	1070, 1780
FbaI	TGATCA	2 cut(s)	1927, 2077
FblI	GTMKAC	3 cut(s)	327, 483, 1628
FokI	GGATG	3 cut(s)	258, 572, 1361
FriOI	GRGCYC	3 cut(s)	13, 102, 1431
FspBI	CTAG	1 cut(s)	2138
GsaI	CCCAGC	2 cut(s)	1400, 1495
GsuI	CTGGAG	2 cut(s)	371, 1586
HaeIII	GGCC	5 cut(s)	209, 408, 905, 1587, 1923
HapII	CCGG	4 cut(s)	377, 625, 1588, 1633
HgaI	GACGC	2 cut(s)	30, 310
Hin1I	GRCGYC	1 cut(s)	1866
HincII	GTYRAC	1 cut(s)	1517
HindII	GTYRAC	1 cut(s)	1517
HinfI	GANTC	7 cut(s)	59, 110, 170, 675, 985, 1275, 1649
HpaII	CCGG	4 cut(s)	377, 625, 1588, 1633
HphI	GGTGA	8 cut(s)	41, 97, 607, 833, 1352, 1394, 1997, 2079
Hpy166II	GTNNAC	7 cut(s)	300, 328, 484, 690, 1385, 1517, 1629
Hpy8I	GTNNAC	7 cut(s)	300, 328, 484, 690, 1385, 1517, 1629
Hpy99I	CGWCG	5 cut(s)	98, 124, 127, 452, 536
HpyAV	CCTTC	7 cut(s)	68, 346, 782, 1328, 1395, 1785, 2105
HpyCH4III	ACNGT	9 cut(s)	132, 193, 910, 1097, 1146, 1154, 1521, 1548, 2005
HpyCH4IV	ACGT	3 cut(s)	291, 1744, 1866
HpyF10VI	GCNNNNNNNGC	3 cut(s)	212, 1435, 2006
HpyF3I	CTNAG	3 cut(s)	42, 620, 1579
HpySE526I	ACGT	3 cut(s)	291, 1744, 1866
Hsp92I	GRCGYC	1 cut(s)	1866
KroI	GCCGGC	1 cut(s)	1587
KroNI	GCCGGC	1 cut(s)	1589
Ksp22I	TGATCA	2 cut(s)	1927, 2077
Kzo9I	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
LmnI	GCTCC	5 cut(s)	8, 38, 1434, 1754, 2150
Lsp1109I	GCAGC	7 cut(s)	389, 727, 1156, 1699, 1795, 1798, 2089
LweI	GCATC	5 cut(s)	550, 1823, 1969, 1970, 2134
MaeI	CTAG	1 cut(s)	2138
MaeII	ACGT	3 cut(s)	291, 1744, 1866
MaeIII	GTNAC	7 cut(s)	47, 637, 791, 821, 866, 895, 1007
MalI	GATC	9 cut(s)	28, 489, 855, 1036, 1359, 1767, 1929, 2079, 2086
MboI	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
MflI	RGATCY	1 cut(s)	1765
MhlI	GDGCHC	5 cut(s)	13, 102, 631, 1268, 1431
MlsI	TGGCCA	2 cut(s)	905, 1923
MluCI	AATT	9 cut(s)	244, 656, 679, 696, 1220, 1299, 1705, 2017, 2071
MluNI	TGGCCA	2 cut(s)	905, 1923
MlyI	GAGTC	2 cut(s)	53, 179
Mox20I	TGGCCA	2 cut(s)	905, 1923
Mph1103I	ATGCAT	1 cut(s)	6
MroNI	GCCGGC	1 cut(s)	1587
MroXI	GAANNNNTTC	1 cut(s)	386
MscI	TGGCCA	2 cut(s)	905, 1923
MseI	TTAA	2 cut(s)	1125, 1257
Msp20I	TGGCCA	2 cut(s)	905, 1923
MspA1I	CMGCKG	2 cut(s)	402, 740
MspI	CCGG	4 cut(s)	377, 625, 1588, 1633
MspR9I	CCNGG	3 cut(s)	556, 626, 1633
MvaI	CCWGG	1 cut(s)	556
MvnI	CGCG	1 cut(s)	459
MwoI	GCNNNNNNNGC	3 cut(s)	212, 1435, 2006
NaeI	GCCGGC	1 cut(s)	1589
NciI	CCSGG	2 cut(s)	626, 1633
NcoI	CCATGG	2 cut(s)	900, 1378
NdeII	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
NgoMIV	GCCGGC	1 cut(s)	1587
NlaIV	GGNNCC	1 cut(s)	1796
NmeAIII	GCCGAG	1 cut(s)	522
NmuCI	GTSAC	2 cut(s)	47, 821
NruI	TCGCGA	1 cut(s)	459
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	3 cut(s)	932, 1808, 2099
PaeI	GCATGC	1 cut(s)	1808
PagI	TCATGA	1 cut(s)	1303
PasI	CCCWGGG	1 cut(s)	555
PciI	ACATGT	1 cut(s)	2095
PcsI	WCGNNNNNNNCGW	5 cut(s)	93, 119, 432, 456, 1741
PdiI	GCCGGC	1 cut(s)	1589
PdmI	GAANNNNTTC	1 cut(s)	386
PfeI	GAWTC	5 cut(s)	110, 675, 985, 1275, 1649
Ple19I	CGATCG	1 cut(s)	490
PleI	GAGTC	2 cut(s)	53, 178
PpsI	GAGTC	2 cut(s)	53, 178
Ppu21I	YACGTR	1 cut(s)	292
PpuMI	RGGWCCY	1 cut(s)	1794
PscI	ACATGT	1 cut(s)	2095
Psp124BI	GAGCTC	3 cut(s)	13, 102, 1431
Psp1406I	AACGTT	1 cut(s)	1744
Psp5II	RGGWCCY	1 cut(s)	1794
Psp6I	CCWGG	1 cut(s)	554
PspEI	GGTNACC	1 cut(s)	47
PspFI	CCCAGC	2 cut(s)	1396, 1491
PspGI	CCWGG	1 cut(s)	554
PspN4I	GGNNCC	1 cut(s)	1796
PspPI	GGNCC	3 cut(s)	1069, 1794, 2023
PspPPI	RGGWCCY	1 cut(s)	1794
PsuI	RGATCY	1 cut(s)	1765
PvuI	CGATCG	1 cut(s)	490
PvuII	CAGCTG	2 cut(s)	402, 740
RruI	TCGCGA	1 cut(s)	459
RsaI	GTAC	4 cut(s)	290, 602, 1826, 2177
RsaNI	GTAC	4 cut(s)	289, 601, 1825, 2176
SacI	GAGCTC	3 cut(s)	13, 102, 1431
SaqAI	TTAA	2 cut(s)	1125, 1257
Sau3AI	GATC	9 cut(s)	26, 487, 853, 1034, 1357, 1765, 1927, 2077, 2084
Sau96I	GGNCC	3 cut(s)	1069, 1794, 2023
SchI	GAGTC	2 cut(s)	53, 179
ScrFI	CCNGG	3 cut(s)	556, 626, 1633
SduI	GDGCHC	5 cut(s)	13, 102, 631, 1268, 1431
SfaNI	GCATC	5 cut(s)	550, 1823, 1969, 1970, 2134
SfcI	CTRYAG	4 cut(s)	826, 1269, 1450, 2004
SinI	GGWCC	3 cut(s)	1069, 1794, 2023
SmlI	CTYRAG	4 cut(s)	12, 515, 1433, 1610
SmoI	CTYRAG	4 cut(s)	12, 515, 1433, 1610
SphI	GCATGC	1 cut(s)	1808
Sse9I	AATT	9 cut(s)	244, 656, 679, 696, 1220, 1299, 1705, 2017, 2071
SsiI	CCGC	9 cut(s)	52, 206, 405, 439, 475, 493, 593, 1016, 1067
SspMI	CTAG	1 cut(s)	2138
SstI	GAGCTC	3 cut(s)	13, 102, 1431
StyD4I	CCNGG	3 cut(s)	554, 624, 1631
StyI	CCWWGG	2 cut(s)	900, 1378
TaaI	ACNGT	9 cut(s)	132, 193, 910, 1097, 1146, 1154, 1521, 1548, 2005
TaiI	ACGT	3 cut(s)	294, 1747, 1869
TaqI	TCGA	8 cut(s)	87, 113, 125, 396, 426, 534, 1735, 1851
TasI	AATT	9 cut(s)	244, 656, 679, 696, 1220, 1299, 1705, 2017, 2071
TauI	GCSGC	4 cut(s)	209, 408, 495, 1019
TfiI	GAWTC	5 cut(s)	110, 675, 985, 1275, 1649
Tru1I	TTAA	2 cut(s)	1125, 1257
Tru9I	TTAA	2 cut(s)	1125, 1257
TscAI	CASTG	2 cut(s)	196, 939
TseFI	GTSAC	2 cut(s)	47, 821
TseI	GCWGC	7 cut(s)	402, 740, 1169, 1712, 1808, 1811, 2102
Tsp45I	GTSAC	2 cut(s)	47, 821
TspGWI	ACGGA	1 cut(s)	646
TspRI	CASTG	2 cut(s)	196, 939
VpaK11BI	GGWCC	3 cut(s)	1069, 1794, 2023
XagI	CCTNNNNNAGG	1 cut(s)	1610
XapI	RAATTY	2 cut(s)	1299, 2017
XceI	RCATGY	3 cut(s)	932, 1808, 2099
XmiI	GTMKAC	3 cut(s)	327, 483, 1628
XmnI	GAANNNNTTC	1 cut(s)	386
XspI	CTAG	1 cut(s)	2138
ZraI	GACGTC	1 cut(s)	1867
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.