Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001450

Physical Location & Seq

Genomic Coordinates Forward (+)

31857192 .. 31863313

Gene Structure 6122 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1239500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGCATGGAGCTGAAAGGAGAAAATCGTCAGGAGGAGGTGCTCATGATCAGTACGAAGATGAGGGGTCGACGTTTGAGGATGATGATGATTATGAGGAGGAGCATATTAATGATGATTCTAAGAAACAGTGGCTGTGGAAATTAGGGCGTTTTATCGCTTGTACGACTGCCGGCGACTCCACCACTCTTACTACTACTCCTGGCAGTACAAGTACTGGAAGCAGATCATCGTCTGCATCTTCGTTTCTTGATCGACTCGTTATTCATCCTGATAACTGGTTGTACGTGGGGTGGACGCATTTCATTCTGCTATGGGCAGTCTATTCGTCCTTCTTCACGCCATTGGAGTTCGGCTTCTTCCGGGGTCTGCCCGAGAATCTGTTCCTGCTCGACATCGCCGGCCAGCTGGCCTTCCTTGTCGACATCGTCGTACGCTTCTTCGTGGCCTACCGAGACTTTCACTCGCACCGTATTGTCTACAACCGCAACCTCATCGCTCTCCGCTACTTGAAGTCTCGATTTCTCGTTGATTTGCTCGGTTGTCTGCCTTGGGACGCCATCTACAAGGCTTCTGGGAAAGAAGAGGGAGTGAGGTACCTGTTATGGATAAGACTAAGTCGAGCACGCAGAGTAACAGAGTTTTTTGAGAAGTTGGAGAAAGATATAAGGATCAACTACCTTTTTACAAGAATTGCCAAACTTTTTGTTGTGGAACTTTATAGCACACACACGGCTGCCTGCATCTTTTATTACCTTGCTACTACCATACCTCCGTCACGGGAAGGATACACATGGATTGGAAGTTTGAAGATGGGTGACTATAGCTATTCCCATTTTAGAGAAATTGACCTTTGGAAGCGTTACGTTACCTCACTCTATTTCGCCGTTGTTACCATGGCAACCGTTGGATATGGAGAGATACATGCAGTGAATGTGAGGGAAATGATATTCATCATGTTTTATGTGTCTTTTGACATGCTTTTGGGTGCTTATCTGCTGGGTAACATTACCGCACTGATTGTAAAAGGATCAAAAACTGAAAAGTTTAGGGACAAGATGGCCGAACTTATAAAATACATGAAGAAAAACAGGCTTGATAGGGGGATAAGTAAGGATATCAAAAATCATTTGAGGTTACAGTATGATCGTAGCTACACCGAAGCGGCTGTTCTTCAGGATATTCCAGCCTCCATTCGCAGCAAGATTTCACAGAAATTATATGAACCATACATCAAAGAAGTTTCTCTTTTTAAGGGATGCTCTTTAGGATTCATAAAGCAGATTGCAACCAAAATTCATGAGGAGTTTTTCCTTCCAGGAGAAGTGATCATAGAGCAGGGACAAGCAGTGGATCAATTATACATAGTATGCCATGGGGAGCTGGAGAAGGTGAGAAGGGATGAAGACAGTGAAAGTGAAGAGCTCCTTGAGCGCCTGCAAACCTATAGCTCGTTTGGCGAGGTTTCTTTTCTTTGTAATACTCCCCAGCCATATTCTGTTCGGGCTAGTCAACTGTGCAGGGTCTTGCGACTTGATAAACAATCTTTTAGGGAAATCCTGGATATACATTTCTTAGATGGGCGGATTATCCTAAACAACCTCCTCGAGGGAAAAGACGTGAATACCCGGAACAAGCTTTTGGAATCAGATATCATCCTATATATTGGAAAGCATGAAATGGAGTTAGTTATGAAAGTAAATTGTGCTGCCTATGATGGAGATTTTTATCGAGTAAAACGTTTGATTGGAGCTGGAGCTGATCCCAACAAGATGGACTATGACGGAAGGTCGCCTTTGCATGTTGCTGCATCAAAAGGGTTTGTTGATATCACTCGTTTCCTTATTGAACAAGGAGCGGCCGCTGAGATGTCAGATAAATATGGGAACACTCCTTTACTTGAAGCCATTAAAAATGGGCACGACGAAGTAGGTTCTTTACTTGTTAAGGCGGGGGCATCACTCGCTGTTGATGATGCCGGTGATTTTCTCTGTACCACGGCAGCAAGGAGGAATATGGAGCTTCTGAAAAGGCTTTTATCCAATGACATAAACCCGAATGCGAAAAATTATGATCAGCGGTCACCACTTCATGTAGCTGCTTCAGAAGGCTTATATCCAATGGCAGAATTCCTTTTAGAAGCAGGAGCAAGTGTTCTATCAAAGGACAGATGGGGAAGAACTCCACTTGATGAAGCCCGCATAGGTGGAAATAAGAAACTGATCAAATTGCTTGAAGTCGCCAGAGCTTCTCAGTTGTCAGATTTCTCCGATCATTCTCAAGAAACTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015271 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

764

Amino Acids

86.98

Weight (kDa)

6.3

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	98 - 345	6.4e-26	Ion transport protein
Ion_trans_2	PF07885	285 - 341	1.4e-08	Ion channel
cNMP_binding	PF00027	435 - 521	5.2e-19	Cyclic nucleotide-binding domain
Ank_4	PF13637	568 - 615	2.2e-10	Ankyrin repeats (many copies)
Ank_2	PF12796	569 - 655	7.1e-17	Ankyrin repeats (3 copies)
Ank_5	PF13857	585 - 632	1.8e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	667 - 744	6.1e-09	Ankyrin repeats (3 copies)
Ank_4	PF13637	667 - 712	2.1e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	678 - 732	2.2e-09	Ankyrin repeats (many copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02850 AT3G02850 AT3G02850 AT5G37500 AT5G37500 AT5G37500 AT5G37500
fragaria_vesca	FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_1g30020 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700 FvH4_5g17700
malus_domestica	MD09G1267200.v1.1 MD09G1267300.v1.1 MD14G1239500.v1.1 MD17G1255200.v1.1
prunus_persica	Prupe.3G164900_v2.0.a1 Prupe.3G164900_v2.0.a1 Prupe.5G237000_v2.0.a1 Prupe.5G237000_v2.0.a1
pyrus_communis	pycom09g18000 pycom14g20040 pycom14g20050 pycom17g26330
rosa_chinensis	RchiOBHm_Chr3g0492241 RchiOBHm_Chr7g0177951
rosa_laevigata	RLG00000005488 RLG00000022790
rosa_multiflora	Rmu_co8354969.1_g000001 Rmu_sc0003579.1_g000005 Rmu_sc0017005.1_g000005
rosa_roxburghii	Rroxscaffold_3G00275370 Rroxscaffold_6G00392480
rosa_rugosa	Rorug03G0252500 Rorug03G0252600 Rorug06G0411600.1 Rorug06G0411700.1
rosa_samantha	Rh3AG302600 Rh3BG338200 Rh3CG335700 Rh3DG337800 Rh7AG010400 Rh7BG010600 Rh7CG011000 Rh7DG010700
rosa_wichuraiana	Rw3G026690 Rw7G000890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1070
AbsI	CCTCGAGG	1 cut(s)	1604
Acc65I	GGTACC	1 cut(s)	594
AccB1I	GGYRCC	1 cut(s)	594
AccBSI	CCGCTC	1 cut(s)	1856
AccI	GTMKAC	3 cut(s)	68, 420, 477
AclI	AACGTT	1 cut(s)	1738
AclWI	GGATC	4 cut(s)	677, 1036, 1359, 1754
AcoI	YGGCCR	3 cut(s)	400, 1059, 1857
AcsI	RAATTY	2 cut(s)	1293, 2126
AcuI	CTGAAG	2 cut(s)	1157, 2085
AcyI	GRCGYC	1 cut(s)	555
AfaI	GTAC	8 cut(s)	53, 163, 208, 214, 284, 432, 596, 1993
AfiI	CCNNNNNNNGG	1 cut(s)	1606
AgsI	TTSAA	5 cut(s)	511, 808, 1847, 1901, 2234
AhdI	GACNNNNNGTC	1 cut(s)	1786
AjiI	CACGTC	1 cut(s)	1618
AjnI	CCWGG	3 cut(s)	199, 1315, 1557
AjuI	GAANNNNNNNTTGG	2 cut(s)	1622, 1654
AloI	GAACNNNNNNTCC	2 cut(s)	2136, 2168
Alw21I	GWGCWC	3 cut(s)	43, 625, 1425
Alw26I	GTCTC	2 cut(s)	447, 519
AlwI	GGATC	4 cut(s)	677, 1036, 1359, 1754
AlwNI	CAGNNNCTG	1 cut(s)	133
Ama87I	CYCGRG	2 cut(s)	371, 1604
AoxI	GGCC	5 cut(s)	400, 408, 444, 1059, 1857
ApeKI	GCWGC	6 cut(s)	734, 1197, 1706, 1805, 2000, 2096
ApoI	RAATTY	2 cut(s)	1293, 2126
AseI	ATTAAT	1 cut(s)	108
Asp700I	GAANNNNTTC	1 cut(s)	380
Asp718I	GGTACC	1 cut(s)	594
AspLEI	GCGC	1 cut(s)	1434
AsuC2I	CCSGG	2 cut(s)	362, 1627
AsuHPI	GGTGA	4 cut(s)	827, 1402, 1991, 2073
AvaI	CYCGRG	2 cut(s)	371, 1604
BaeGI	GKGCMC	1 cut(s)	1920
BanI	GGYRCC	1 cut(s)	594
BanII	GRGCYC	1 cut(s)	1425
BbsI	GAAGAC	1 cut(s)	1410
Bbv12I	GWGCWC	3 cut(s)	43, 625, 1425
BbvI	GCAGC	6 cut(s)	721, 1209, 1693, 1792, 2012, 2083
BccI	CCATC	7 cut(s)	566, 805, 1051, 1571, 1709, 1765, 2163
BceAI	ACGGC	3 cut(s)	747, 869, 2013
BcgI	CGANNNNNNTGC	4 cut(s)	306, 340, 475, 509
BciT130I	CCWGG	3 cut(s)	201, 1317, 1559
BciVI	GTATCC	1 cut(s)	779
BclI	TGATCA	4 cut(s)	46, 1326, 2071, 2220
BcnI	CCSGG	2 cut(s)	362, 1627
BcoDI	GTCTC	2 cut(s)	447, 519
BfaI	CTAG	1 cut(s)	1506
BfmI	CTRYAG	2 cut(s)	820, 1444
BfoI	RGCGCY	1 cut(s)	1435
BfuI	GTATCC	1 cut(s)	779
BisI	GCNGC	9 cut(s)	735, 1164, 1198, 1707, 1806, 1857, 1860, 2001, 2097
BlsI	GCNGC	9 cut(s)	736, 1165, 1199, 1708, 1807, 1858, 1861, 2002, 2098
BmcAI	AGTACT	1 cut(s)	214
Bme1390I	CCNGG	5 cut(s)	201, 362, 1317, 1559, 1627
BmeRI	GACNNNNNGTC	1 cut(s)	1786
BmeT110I	CYCGRG	2 cut(s)	371, 1604
BmgBI	CACGTC	1 cut(s)	1618
BmiI	GGNNCC	1 cut(s)	596
BmrFI	CCNGG	5 cut(s)	201, 362, 1317, 1559, 1627
BmsI	GCATC	6 cut(s)	245, 750, 1247, 1817, 1963, 1964
BpiI	GAAGAC	1 cut(s)	1410
BpmI	CTGGAG	2 cut(s)	1403, 1773
BpuEI	CTTGAG	2 cut(s)	1448, 2262
BpuMI	CCSGG	2 cut(s)	362, 1627
BsaAI	YACGTR	1 cut(s)	286
BsaBI	GATNNNNATC	2 cut(s)	950, 1725
BsaHI	GRCGYC	1 cut(s)	555
BsaJI	CCNNGG	5 cut(s)	361, 548, 894, 1372, 1995
BsaXI	ACNNNNNCTCC	6 cut(s)	181, 211, 754, 784, 2136, 2166
Bsc4I	CCNNNNNNNGG	1 cut(s)	1606
Bse118I	RCCGGY	3 cut(s)	170, 398, 1976
Bse1I	ACTGG	3 cut(s)	220, 281, 2290
Bse8I	GATNNNNATC	2 cut(s)	950, 1725
BseBI	CCWGG	3 cut(s)	201, 1317, 1559
BseDI	CCNNGG	5 cut(s)	361, 548, 894, 1372, 1995
BseGI	GGATG	5 cut(s)	85, 265, 1262, 1405, 1653
BseJI	GATNNNNATC	2 cut(s)	950, 1725
BseLI	CCNNNNNNNGG	1 cut(s)	1606
BseMII	CTCAG	2 cut(s)	1854, 2264
BseNI	ACTGG	3 cut(s)	220, 281, 2290
BseRI	GAGGAG	5 cut(s)	48, 110, 113, 1316, 1592
BseSI	GKGCMC	1 cut(s)	1920
BseX3I	CGGCCG	1 cut(s)	1857
BseXI	GCAGC	6 cut(s)	721, 1209, 1693, 1792, 2012, 2083
BseYI	CCCAGC	2 cut(s)	997, 1485
BsgI	GTGCAG	1 cut(s)	1537
Bsh1285I	CGRYCG	1 cut(s)	1860
BshFI	GGCC	5 cut(s)	402, 410, 446, 1061, 1859
BshNI	GGYRCC	1 cut(s)	594
BsiEI	CGRYCG	1 cut(s)	1860
BsiHKAI	GWGCWC	3 cut(s)	43, 625, 1425
BsiHKCI	CYCGRG	2 cut(s)	371, 1604
BsiSI	CCGG	5 cut(s)	171, 361, 399, 1627, 1977
BsiWI	CGTACG	1 cut(s)	430
BslFI	GGGAC	3 cut(s)	566, 1064, 1353
BslI	CCNNNNNNNGG	1 cut(s)	1606
BsmAI	GTCTC	2 cut(s)	447, 519
BsmFI	GGGAC	3 cut(s)	566, 1064, 1353
BsmI	GAATGC	1 cut(s)	2062
BsnI	GGCC	5 cut(s)	402, 410, 446, 1061, 1859
BsoBI	CYCGRG	2 cut(s)	371, 1604
Bsp1286I	GDGCHC	4 cut(s)	43, 625, 1425, 1920
Bsp19I	CCATGG	2 cut(s)	894, 1372
BspANI	GGCC	5 cut(s)	402, 410, 446, 1061, 1859
BspCNI	CTCAG	2 cut(s)	1855, 2263
BspHI	TCATGA	2 cut(s)	43, 1297
BspLI	GGNNCC	1 cut(s)	596
BspPI	GGATC	4 cut(s)	677, 1036, 1359, 1754
BspQI	GCTCTTC	1 cut(s)	1413
BspT107I	GGYRCC	1 cut(s)	594
BsrBI	CCGCTC	1 cut(s)	1856
BsrFI	RCCGGY	3 cut(s)	170, 398, 1976
BsrI	ACTGG	3 cut(s)	220, 281, 2290
BssAI	RCCGGY	3 cut(s)	170, 398, 1976
BssECI	CCNNGG	5 cut(s)	361, 548, 894, 1372, 1995
BssNI	GRCGYC	1 cut(s)	555
BssT1I	CCWWGG	3 cut(s)	548, 894, 1372
Bst2UI	CCWGG	3 cut(s)	201, 1317, 1559
Bst4CI	ACNGT	6 cut(s)	129, 470, 904, 1140, 1409, 1515
Bst6I	CTCTTC	2 cut(s)	576, 1413
BstACI	GRCGYC	1 cut(s)	555
BstBAI	YACGTR	1 cut(s)	286
BstC8I	GCNNGC	8 cut(s)	172, 400, 404, 408, 625, 739, 1436, 2197
BstDEI	CTNAG	5 cut(s)	120, 614, 1573, 1863, 2250
BstDSI	CCRYGG	3 cut(s)	894, 1372, 1995
BstEII	GGTNACC	1 cut(s)	2079
BstENI	CCTNNNNNAGG	1 cut(s)	1604
BstF5I	GGATG	5 cut(s)	85, 265, 1262, 1405, 1653
BstH2I	RGCGCY	1 cut(s)	1435
BstHHI	GCGC	1 cut(s)	1434
BstMAI	GTCTC	2 cut(s)	447, 519
BstMCI	CGRYCG	1 cut(s)	1860
BstMWI	GCNNNNNNNGC	3 cut(s)	1429, 1455, 1961
BstNI	CCWGG	3 cut(s)	201, 1317, 1559
BstNSI	RCATGY	3 cut(s)	926, 979, 1802
BstPI	GGTNACC	1 cut(s)	2079
BstSCI	CCNGG	5 cut(s)	199, 360, 1315, 1557, 1625
BstSFI	CTRYAG	2 cut(s)	820, 1444
BstSLI	GKGCMC	1 cut(s)	1920
BstV1I	GCAGC	6 cut(s)	721, 1209, 1693, 1792, 2012, 2083
BstV2I	GAAGAC	1 cut(s)	1410
BstXI	CCANNNNNNTGG	1 cut(s)	1771
BstZI	CGGCCG	1 cut(s)	1857
BsuI	GTATCC	1 cut(s)	779
BsuRI	GGCC	5 cut(s)	402, 410, 446, 1061, 1859
BtgI	CCRYGG	3 cut(s)	894, 1372, 1995
BtgZI	GCGATG	2 cut(s)	379, 478
BtrI	CACGTC	1 cut(s)	1618
BtsCI	GGATG	5 cut(s)	85, 265, 1262, 1405, 1653
BtsI	GCAGTG	2 cut(s)	933, 1353
BtsIMutI	CAGTG	5 cut(s)	134, 933, 1013, 1353, 1414
Cac8I	GCNNGC	8 cut(s)	172, 400, 404, 408, 625, 739, 1436, 2197
CaiI	CAGNNNCTG	1 cut(s)	133
CciI	TCATGA	2 cut(s)	43, 1297
CciNI	GCGGCCGC	1 cut(s)	1857
CfoI	GCGC	1 cut(s)	1434
Cfr10I	RCCGGY	3 cut(s)	170, 398, 1976
CseI	GACGC	2 cut(s)	304, 563
Csp6I	GTAC	8 cut(s)	52, 162, 207, 213, 283, 431, 595, 1992
CviQI	GTAC	8 cut(s)	52, 162, 207, 213, 283, 431, 595, 1992
DdeI	CTNAG	5 cut(s)	120, 614, 1573, 1863, 2250
DriI	GACNNNNNGTC	1 cut(s)	1786
EaeI	YGGCCR	3 cut(s)	400, 1059, 1857
EagI	CGGCCG	1 cut(s)	1857
Eam1104I	CTCTTC	2 cut(s)	576, 1413
Eam1105I	GACNNNNNGTC	1 cut(s)	1786
EarI	CTCTTC	2 cut(s)	576, 1413
EciI	GGCGGA	1 cut(s)	1597
Ecl136II	GAGCTC	1 cut(s)	1423
EclXI	CGGCCG	1 cut(s)	1857
Eco130I	CCWWGG	3 cut(s)	548, 894, 1372
Eco24I	GRGCYC	1 cut(s)	1425
Eco32I	GATATC	3 cut(s)	1117, 1651, 1828
Eco52I	CGGCCG	1 cut(s)	1857
Eco53kI	GAGCTC	1 cut(s)	1423
Eco57I	CTGAAG	2 cut(s)	1157, 2085
Eco88I	CYCGRG	2 cut(s)	371, 1604
Eco91I	GGTNACC	1 cut(s)	2079
EcoICRI	GAGCTC	1 cut(s)	1423
EcoNI	CCTNNNNNAGG	1 cut(s)	1604
EcoO65I	GGTNACC	1 cut(s)	2079
EcoRI	GAATTC	1 cut(s)	2126
EcoRII	CCWGG	3 cut(s)	199, 1315, 1557
EcoRV	GATATC	3 cut(s)	1117, 1651, 1828
EcoT14I	CCWWGG	3 cut(s)	548, 894, 1372
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	1425
ErhI	CCWWGG	3 cut(s)	548, 894, 1372
FalI	AAGNNNNNCTT	6 cut(s)	1230, 1262, 1410, 1442, 1777, 1809
FaqI	GGGAC	3 cut(s)	566, 1064, 1353
FauI	CCCGC	2 cut(s)	1942, 2204
FbaI	TGATCA	4 cut(s)	46, 1326, 2071, 2220
FblI	GTMKAC	3 cut(s)	68, 420, 477
Fnu4HI	GCNGC	9 cut(s)	735, 1164, 1198, 1707, 1806, 1857, 1860, 2001, 2097
FokI	GGATG	5 cut(s)	92, 252, 1269, 1412, 1640
FriOI	GRGCYC	1 cut(s)	1425
Fsp4HI	GCNGC	9 cut(s)	735, 1164, 1198, 1707, 1806, 1857, 1860, 2001, 2097
FspBI	CTAG	1 cut(s)	1506
GlaI	GCGC	1 cut(s)	1433
GluI	GCNGC	9 cut(s)	735, 1164, 1198, 1707, 1806, 1857, 1860, 2001, 2097
GsaI	CCCAGC	2 cut(s)	1001, 1489
GsuI	CTGGAG	2 cut(s)	1403, 1773
HaeII	RGCGCY	1 cut(s)	1435
HaeIII	GGCC	5 cut(s)	402, 410, 446, 1061, 1859
HapII	CCGG	5 cut(s)	171, 361, 399, 1627, 1977
HgaI	GACGC	2 cut(s)	304, 563
HhaI	GCGC	1 cut(s)	1434
Hin1I	GRCGYC	1 cut(s)	555
Hin6I	GCGC	1 cut(s)	1432
HinP1I	GCGC	1 cut(s)	1432
HincII	GTYRAC	3 cut(s)	69, 421, 1511
HindII	GTYRAC	3 cut(s)	69, 421, 1511
HindIII	AAGCTT	1 cut(s)	1634
HinfI	GANTC	6 cut(s)	116, 176, 255, 376, 1269, 1643
HpaII	CCGG	5 cut(s)	171, 361, 399, 1627, 1977
HphI	GGTGA	4 cut(s)	827, 1402, 1991, 2073
Hpy166II	GTNNAC	5 cut(s)	69, 294, 421, 478, 1511
Hpy188I	TCNGA	6 cut(s)	1648, 1873, 2025, 2104, 2260, 2269
Hpy188III	TCNNGA	8 cut(s)	30, 44, 248, 269, 516, 1175, 1298, 2279
Hpy8I	GTNNAC	5 cut(s)	69, 294, 421, 478, 1511
Hpy99I	CGWCG	3 cut(s)	73, 431, 1925
HpyAV	CCTTC	8 cut(s)	340, 421, 776, 1322, 1381, 1389, 1779, 2099
HpyCH4III	ACNGT	6 cut(s)	129, 470, 904, 1140, 1409, 1515
HpyCH4IV	ACGT	5 cut(s)	71, 285, 864, 1617, 1738
HpyCH4V	TGCA	9 cut(s)	4, 236, 741, 926, 1286, 1438, 1518, 1798, 1808
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1429, 1455, 1961
HpyF3I	CTNAG	5 cut(s)	120, 614, 1573, 1863, 2250
HpySE526I	ACGT	5 cut(s)	71, 285, 864, 1617, 1738
Hsp92I	GRCGYC	1 cut(s)	555
HspAI	GCGC	1 cut(s)	1432
KpnI	GGTACC	1 cut(s)	598
KroI	GCCGGC	2 cut(s)	170, 398
KroNI	GCCGGC	2 cut(s)	172, 400
Ksp22I	TGATCA	4 cut(s)	46, 1326, 2071, 2220
LguI	GCTCTTC	1 cut(s)	1413
LmnI	GCTCC	9 cut(s)	8, 100, 1378, 1428, 1748, 1754, 1853, 2017, 2144
Lsp1109I	GCAGC	6 cut(s)	721, 1209, 1693, 1792, 2012, 2083
LweI	GCATC	6 cut(s)	245, 750, 1247, 1817, 1963, 1964
MaeI	CTAG	1 cut(s)	1506
MaeII	ACGT	5 cut(s)	71, 285, 864, 1617, 1738
MaeIII	GTNAC	9 cut(s)	631, 774, 815, 860, 865, 889, 1001, 1134, 2079
MbiI	CCGCTC	1 cut(s)	1856
MhlI	GDGCHC	4 cut(s)	43, 625, 1425, 1920
MlyI	GAGTC	2 cut(s)	170, 249
MmeI	TCCRAC	2 cut(s)	633, 886
Mph1103I	ATGCAT	1 cut(s)	6
MroNI	GCCGGC	2 cut(s)	170, 398
MroXI	GAANNNNTTC	1 cut(s)	380
MseI	TTAA	5 cut(s)	108, 1251, 1908, 1944, 2290
MslI	CAYNNNNRTG	1 cut(s)	108
MspA1I	CMGCKG	3 cut(s)	406, 1862, 2077
MspI	CCGG	5 cut(s)	171, 361, 399, 1627, 1977
MspR9I	CCNGG	5 cut(s)	201, 362, 1317, 1559, 1627
Mva1269I	GAATGC	1 cut(s)	2062
MvaI	CCWGG	3 cut(s)	201, 1317, 1559
MwoI	GCNNNNNNNGC	3 cut(s)	1429, 1455, 1961
NaeI	GCCGGC	2 cut(s)	172, 400
NciI	CCSGG	2 cut(s)	362, 1627
NcoI	CCATGG	2 cut(s)	894, 1372
NgoMIV	GCCGGC	2 cut(s)	170, 398
NlaIV	GGNNCC	1 cut(s)	596
NmuCI	GTSAC	3 cut(s)	774, 815, 2079
NotI	GCGGCCGC	1 cut(s)	1857
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	3 cut(s)	926, 979, 1802
PaeR7I	CTCGAG	1 cut(s)	1604
PagI	TCATGA	2 cut(s)	43, 1297
PciSI	GCTCTTC	1 cut(s)	1413
PcsI	WCGNNNNNNNCGW	2 cut(s)	426, 1735
PctI	GAATGC	1 cut(s)	2062
PdiI	GCCGGC	2 cut(s)	172, 400
PdmI	GAANNNNTTC	1 cut(s)	380
PfeI	GAWTC	4 cut(s)	116, 376, 1269, 1643
Pfl23II	CGTACG	1 cut(s)	430
PflFI	GACNNNGTC	2 cut(s)	425, 615
PfoI	TCCNGGA	2 cut(s)	1315, 1557
PkrI	GCNGC	9 cut(s)	736, 1165, 1199, 1708, 1807, 1858, 1861, 2002, 2098
PleI	GAGTC	2 cut(s)	170, 249
PpsI	GAGTC	2 cut(s)	170, 249
Ppu21I	YACGTR	1 cut(s)	286
PshBI	ATTAAT	1 cut(s)	108
PsiI	TTATAA	1 cut(s)	1070
Psp124BI	GAGCTC	1 cut(s)	1425
Psp1406I	AACGTT	1 cut(s)	1738
Psp6I	CCWGG	3 cut(s)	199, 1315, 1557
PspEI	GGTNACC	1 cut(s)	2079
PspFI	CCCAGC	2 cut(s)	997, 1485
PspGI	CCWGG	3 cut(s)	199, 1315, 1557
PspLI	CGTACG	1 cut(s)	430
PspN4I	GGNNCC	1 cut(s)	596
PspXI	VCTCGAGB	1 cut(s)	1604
PstNI	CAGNNNCTG	1 cut(s)	133
PsyI	GACNNNGTC	2 cut(s)	425, 615
PvuII	CAGCTG	1 cut(s)	406
RsaI	GTAC	8 cut(s)	53, 163, 208, 214, 284, 432, 596, 1993
RsaNI	GTAC	8 cut(s)	52, 162, 207, 213, 283, 431, 595, 1992
RseI	CAYNNNNRTG	1 cut(s)	108
SacI	GAGCTC	1 cut(s)	1425
SalI	GTCGAC	2 cut(s)	67, 419
SapI	GCTCTTC	1 cut(s)	1413
SaqAI	TTAA	5 cut(s)	108, 1251, 1908, 1944, 2290
SatI	GCNGC	9 cut(s)	735, 1164, 1198, 1707, 1806, 1857, 1860, 2001, 2097
ScaI	AGTACT	1 cut(s)	214
SchI	GAGTC	2 cut(s)	170, 249
ScrFI	CCNGG	5 cut(s)	201, 362, 1317, 1559, 1627
SduI	GDGCHC	4 cut(s)	43, 625, 1425, 1920
SfaNI	GCATC	6 cut(s)	245, 750, 1247, 1817, 1963, 1964
SfcI	CTRYAG	2 cut(s)	820, 1444
Sfr274I	CTCGAG	1 cut(s)	1604
SlaI	CTCGAG	1 cut(s)	1604
SmiMI	CAYNNNNRTG	1 cut(s)	108
SmlI	CTYRAG	3 cut(s)	1427, 1604, 2277
SmoI	CTYRAG	3 cut(s)	1427, 1604, 2277
SspMI	CTAG	1 cut(s)	1506
SstI	GAGCTC	1 cut(s)	1425
StyD4I	CCNGG	5 cut(s)	199, 360, 1315, 1557, 1625
StyI	CCWWGG	3 cut(s)	548, 894, 1372
TaaI	ACNGT	6 cut(s)	129, 470, 904, 1140, 1409, 1515
TaiI	ACGT	5 cut(s)	74, 288, 867, 1620, 1741
TaqI	TCGA	8 cut(s)	68, 253, 390, 420, 517, 619, 1605, 1729
TatI	WGTACW	2 cut(s)	206, 212
TauI	GCSGC	3 cut(s)	1166, 1859, 1862
TfiI	GAWTC	4 cut(s)	116, 376, 1269, 1643
Tru1I	TTAA	5 cut(s)	108, 1251, 1908, 1944, 2290
Tru9I	TTAA	5 cut(s)	108, 1251, 1908, 1944, 2290
TscAI	CASTG	5 cut(s)	134, 933, 1020, 1353, 1414
TseFI	GTSAC	3 cut(s)	774, 815, 2079
TseI	GCWGC	6 cut(s)	734, 1197, 1706, 1805, 2000, 2096
Tsp45I	GTSAC	3 cut(s)	774, 815, 2079
TspGWI	ACGGA	2 cut(s)	762, 1797
TspRI	CASTG	5 cut(s)	134, 933, 1020, 1353, 1414
Tth111I	GACNNNGTC	2 cut(s)	425, 615
VspI	ATTAAT	1 cut(s)	108
XagI	CCTNNNNNAGG	1 cut(s)	1604
XapI	RAATTY	2 cut(s)	1293, 2126
XceI	RCATGY	3 cut(s)	926, 979, 1802
XhoI	CTCGAG	1 cut(s)	1604
XmiI	GTMKAC	3 cut(s)	68, 420, 477
XmnI	GAANNNNTTC	1 cut(s)	380
XspI	CTAG	1 cut(s)	1506
ZrmI	AGTACT	1 cut(s)	214
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.