MD10G1225900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003267
Physical Location & Seq
Forward (+)
32324476 .. 32326264
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1225900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGCAATCCCTCGTCATCTCTCTCTCCAACCATCTTATAAAACCCAAAAGCAAATACCACGCCTCCTTCTGTTGCTTTCTCAAAACCTACAATTCATGGGCTTCCGCTATCCGAAATGCTTCTTCCCCCCACAATGCTCTGCACATCTACTCTCAAATGCACCGAAATTCGATTCCTTTCGATACCTTCTCCGTCCTCTTTACGCTAAAATCGTGTACCCAGTTGAAGAACCACGCAATCATTCAACACCTCCATGCCCATGTAATCAAACTTGGGTTTTGCTCGCATGTATTTGTGGCCACTTCACTTCTCCATGCGTACGTTGTTGTGTCGTTTGTGGATGCATGCCTACTGTTCGATGAAATTCCTGAGAAGAACACGGTCACGTGGAACACGATGATTGCGGGGTTTTCGAGGTCGGGGGATGTGGAAAGAGCGCGTTTGGTTTTTGAGGAAATGCCGTTGAGAGATGTTGCGTCGTGGTCCGCCATGATAGCTGCGTACGTGAACAATGGCAAGCATGAGTGTGGTTTATCGTTGTTTCGGGATATGGTGATGGATGAAGGTTTGAAGCCTGACCAAGTGAGTGCGGGCTCAATTTTATCGGGTTGTGCCCACTTGGGTTCTCTTGGTTTGTTGGTGGGGAAATCAGTTCATGGGTTTATGGCTAAGAACGGGTGGGAATTGAATGTGGAAATTGGTACGATATTGGTTGACATGTATGCTAAGTGTGGGTTTTTGAAGGGAGCTGCACAGGTTTTTGAATTGATGCAAGAAAGGAATGTCATGACTTGGACCGCTTTGATTTGTGGATCGGCACAACATGGCTACAGCGAAGCAGCATTGTCTTTGTTTGAGATGATGCAAAAGGCCAGTGTAAGAGCTAATGAACTGACCTTTACTGGAGTTCTGAGTGCTTGTGTGCATACAGGACTAGTTGAGGAGGGCCGGAAATATTTCAAGATGATCGAAGAAAGTGGCTTGGAGCCTAGAATACATCATTACGGATGCATGGTTGATTTGTACGGCAAGGCAGGACTGTTAGAGGAAGCTTATGAGGTTATTATGAAAATGAGTCTTGAGCCCAATGTCGTTATTTGGACTGCTTTTTTATCTGCTTGTAAGGAGCATAAACAGTTTGAGATGGCAGAACGAGTGGTTGAGCAGGTCATGAAGATGGTAAAACCAGAGAACGATGGTGGGGTTTATTCTCTTCTCTCGAATCTGTATGCTTTGGGTGGGAAGTGGGATGATGCAGAAAAAGTGAGGAATTTGATGAACAACCAACGTGTGAGGAAGGTCGGAGCATCTAGTATGGTTAGAAGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

444

Amino Acids

49.3

Weight (kDa)

7.28

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 125 - 156 4.2e-07 PPR repeat family
PPR_1 PF12854 126 - 153 4.4e-07 PPR repeat
PPR PF01535 128 - 156 8.9e-08 PPR repeat
PPR_2 PF13041 156 - 206 2.4e-07 PPR repeat family
PPR_2 PF13041 260 - 308 1.3e-09 PPR repeat family
PPR_long PF17177 269 - 359 3.2e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 377 - 438 7.2e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 1156
AccII CGCG 1 cut(s) 439
AciI CCGC 5 cut(s) 105, 404, 486, 590, 798
AclWI GGATC 1 cut(s) 820
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 3 cut(s) 166, 363, 1270
AcvI CACGTG 1 cut(s) 387
AfaI GTAC 5 cut(s) 217, 320, 503, 703, 1025
AflIII ACRYGT 2 cut(s) 717, 1288
AgsI TTSAA 7 cut(s) 226, 245, 571, 688, 742, 764, 961
AhlI ACTAGT 1 cut(s) 934
AluBI AGCT 4 cut(s) 497, 749, 884, 1052
AluI AGCT 4 cut(s) 497, 749, 884, 1052
AlwI GGATC 1 cut(s) 820
AoxI GGCC 3 cut(s) 297, 870, 946
ApeKI GCWGC 3 cut(s) 497, 749, 839
ApoI RAATTY 3 cut(s) 166, 363, 1270
Asp700I GAANNNNTTC 2 cut(s) 118, 956
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 483, 795, 946
AsuHPI GGTGA 1 cut(s) 565
AvaII GGWCC 2 cut(s) 483, 795
BaeGI GKGCMC 1 cut(s) 616
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 2 cut(s) 596, 1086
BbrPI CACGTG 1 cut(s) 387
BbvI GCAGC 3 cut(s) 484, 736, 851
BccI CCATC 5 cut(s) 39, 550, 1138, 1171, 1190
BceAI ACGGC 2 cut(s) 445, 1042
BcuI ACTAGT 1 cut(s) 934
BfaI CTAG 3 cut(s) 935, 990, 1311
BfmI CTRYAG 1 cut(s) 829
BfuAI ACCTGC 1 cut(s) 1156
BisI GCNGC 3 cut(s) 498, 750, 840
BlsI GCNGC 3 cut(s) 499, 751, 841
Bme18I GGWCC 2 cut(s) 483, 795
BmgT120I GGNCC 3 cut(s) 483, 795, 946
BmiI GGNNCC 1 cut(s) 987
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 6 cut(s) 331, 759, 852, 998, 1243, 1316
BmuI ACTGGG 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 1100
BsaAI YACGTR 2 cut(s) 387, 505
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bse1I ACTGG 3 cut(s) 220, 873, 907
BseGI GGATG 5 cut(s) 346, 430, 565, 1013, 1255
BseMII CTCAG 2 cut(s) 360, 902
BseNI ACTGG 3 cut(s) 220, 873, 907
BseRI GAGGAG 1 cut(s) 956
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 3 cut(s) 484, 736, 851
BsgI GTGCAG 2 cut(s) 125, 735
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 299, 872, 948
BsiSI CCGG 1 cut(s) 949
BsiWI CGTACG 2 cut(s) 318, 501
BsnI GGCC 3 cut(s) 299, 872, 948
Bsp1286I GDGCHC 3 cut(s) 596, 616, 1086
Bsp143I GATC 2 cut(s) 812, 966
BspACI CCGC 5 cut(s) 105, 404, 486, 590, 798
BspANI GGCC 3 cut(s) 299, 872, 948
BspCNI CTCAG 2 cut(s) 361, 903
BspFNI CGCG 1 cut(s) 439
BspHI TCATGA 2 cut(s) 786, 1170
BspLI GGNNCC 1 cut(s) 987
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 1 cut(s) 820
BsrI ACTGG 3 cut(s) 220, 873, 907
BssMI GATC 2 cut(s) 812, 966
Bst4CI ACNGT 4 cut(s) 354, 382, 1041, 1137
Bst6I CTCTTC 1 cut(s) 1218
BstBAI YACGTR 2 cut(s) 387, 505
BstC8I GCNNGC 4 cut(s) 284, 346, 518, 592
BstDEI CTNAG 4 cut(s) 369, 669, 726, 911
BstF5I GGATG 5 cut(s) 346, 430, 565, 1013, 1255
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 2 cut(s) 815, 969
BstMBI GATC 2 cut(s) 812, 966
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNSI RCATGY 3 cut(s) 290, 348, 721
BstSFI CTRYAG 1 cut(s) 829
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 3 cut(s) 484, 736, 851
BsuRI GGCC 3 cut(s) 299, 872, 948
BtsCI GGATG 5 cut(s) 346, 430, 565, 1013, 1255
BtsIMutI CAGTG 1 cut(s) 880
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 4 cut(s) 284, 346, 518, 592
CciI TCATGA 2 cut(s) 786, 1170
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 483, 795, 946
CseI GACGC 1 cut(s) 465
Csp6I GTAC 5 cut(s) 216, 319, 502, 702, 1024
CviQI GTAC 5 cut(s) 216, 319, 502, 702, 1024
DdeI CTNAG 4 cut(s) 369, 669, 726, 911
DpnI GATC 2 cut(s) 814, 968
DpnII GATC 2 cut(s) 812, 966
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
EciI GGCGGA 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 596, 1086
Eco47I GGWCC 2 cut(s) 483, 795
Eco72I CACGTG 1 cut(s) 387
EcoT22I ATGCAT 2 cut(s) 346, 1013
EcoT38I GRGCYC 2 cut(s) 596, 1086
FauI CCCGC 2 cut(s) 397, 583
Fnu4HI GCNGC 3 cut(s) 498, 750, 840
FokI GGATG 5 cut(s) 353, 437, 572, 1020, 1262
FriOI GRGCYC 2 cut(s) 596, 1086
Fsp4HI GCNGC 3 cut(s) 498, 750, 840
FspBI CTAG 3 cut(s) 935, 990, 1311
GlaI GCGC 1 cut(s) 438
GluI GCNGC 3 cut(s) 498, 750, 840
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 3 cut(s) 299, 872, 948
HapII CCGG 1 cut(s) 949
HgaI GACGC 1 cut(s) 465
HhaI GCGC 1 cut(s) 439
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HindIII AAGCTT 1 cut(s) 1050
HinfI GANTC 3 cut(s) 172, 1075, 1222
HpaII CCGG 1 cut(s) 949
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 3 cut(s) 216, 508, 715
Hpy188I TCNGA 3 cut(s) 113, 912, 1304
Hpy188III TCNNGA 7 cut(s) 368, 545, 787, 961, 1079, 1171, 1219
Hpy8I GTNNAC 3 cut(s) 216, 508, 715
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 5 cut(s) 76, 196, 557, 736, 1291
HpyCH4III ACNGT 4 cut(s) 354, 382, 1041, 1137
HpyCH4IV ACGT 4 cut(s) 321, 386, 504, 1288
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 4 cut(s) 369, 669, 726, 911
HpySE526I ACGT 4 cut(s) 321, 386, 504, 1288
HspAI GCGC 1 cut(s) 437
Kzo9I GATC 2 cut(s) 812, 966
LmnI GCTCC 4 cut(s) 746, 985, 1126, 1304
Lsp1109I GCAGC 3 cut(s) 484, 736, 851
LweI GCATC 6 cut(s) 331, 759, 852, 998, 1243, 1316
MaeI CTAG 3 cut(s) 935, 990, 1311
MaeII ACGT 4 cut(s) 321, 386, 504, 1288
MaeIII GTNAC 1 cut(s) 382
MalI GATC 2 cut(s) 814, 968
MboI GATC 2 cut(s) 812, 966
MboII GAAGA 6 cut(s) 114, 238, 385, 983, 1186, 1205
MhlI GDGCHC 3 cut(s) 596, 616, 1086
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 8 cut(s) 91, 166, 363, 597, 683, 696, 764, 1270
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 1 cut(s) 1084
MmeI TCCRAC 2 cut(s) 51, 1282
Mox20I TGGCCA 1 cut(s) 299
Mph1103I ATGCAT 2 cut(s) 346, 1013
MroXI GAANNNNTTC 2 cut(s) 118, 956
MscI TGGCCA 1 cut(s) 299
MslI CAYNNNNRTG 4 cut(s) 252, 258, 525, 1175
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 1 cut(s) 949
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 494
NdeII GATC 2 cut(s) 812, 966
NlaIV GGNNCC 1 cut(s) 987
NmuCI GTSAC 1 cut(s) 382
NsiI ATGCAT 2 cut(s) 346, 1013
NspI RCATGY 3 cut(s) 290, 348, 721
PaeI GCATGC 1 cut(s) 348
PagI TCATGA 2 cut(s) 786, 1170
PciI ACATGT 1 cut(s) 717
PcsI WCGNNNNNNNCGW 2 cut(s) 209, 392
PdmI GAANNNNTTC 2 cut(s) 118, 956
PfeI GAWTC 2 cut(s) 172, 1222
Pfl23II CGTACG 2 cut(s) 318, 501
PkrI GCNGC 3 cut(s) 499, 751, 841
PleI GAGTC 1 cut(s) 1083
PmaCI CACGTG 1 cut(s) 387
PmlI CACGTG 1 cut(s) 387
PpsI GAGTC 1 cut(s) 1083
Ppu21I YACGTR 2 cut(s) 387, 505
PscI ACATGT 1 cut(s) 717
PsiI TTATAA 1 cut(s) 38
PspCI CACGTG 1 cut(s) 387
PspLI CGTACG 2 cut(s) 318, 501
PspN4I GGNNCC 1 cut(s) 987
PspPI GGNCC 3 cut(s) 483, 795, 946
RsaI GTAC 5 cut(s) 217, 320, 503, 703, 1025
RsaNI GTAC 5 cut(s) 216, 319, 502, 702, 1024
RseI CAYNNNNRTG 4 cut(s) 252, 258, 525, 1175
SatI GCNGC 3 cut(s) 498, 750, 840
Sau3AI GATC 2 cut(s) 812, 966
Sau96I GGNCC 3 cut(s) 483, 795, 946
SchI GAGTC 1 cut(s) 1084
SduI GDGCHC 3 cut(s) 596, 616, 1086
SfaNI GCATC 6 cut(s) 331, 759, 852, 998, 1243, 1316
SfcI CTRYAG 1 cut(s) 829
SinI GGWCC 2 cut(s) 483, 795
SmiMI CAYNNNNRTG 4 cut(s) 252, 258, 525, 1175
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
SpeI ACTAGT 1 cut(s) 934
SphI GCATGC 1 cut(s) 348
Sse9I AATT 8 cut(s) 91, 166, 363, 597, 683, 696, 764, 1270
SsiI CCGC 5 cut(s) 105, 404, 486, 590, 798
SspI AATATT 1 cut(s) 956
SspMI CTAG 3 cut(s) 935, 990, 1311
TaaI ACNGT 4 cut(s) 354, 382, 1041, 1137
TaiI ACGT 4 cut(s) 324, 389, 507, 1291
TaqI TCGA 6 cut(s) 170, 180, 357, 413, 969, 1220
TasI AATT 8 cut(s) 91, 166, 363, 597, 683, 696, 764, 1270
TfiI GAWTC 2 cut(s) 172, 1222
TscAI CASTG 1 cut(s) 880
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 3 cut(s) 497, 749, 839
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 8 cut(s) 84, 375, 576, 644, 903, 1082, 1187, 1292
TspGWI ACGGA 2 cut(s) 181, 1020
TspRI CASTG 1 cut(s) 880
VpaK11BI GGWCC 2 cut(s) 483, 795
XapI RAATTY 3 cut(s) 166, 363, 1270
XceI RCATGY 3 cut(s) 290, 348, 721
XmnI GAANNNNTTC 2 cut(s) 118, 956
XspI CTAG 3 cut(s) 935, 990, 1311
Zsp2I ATGCAT 2 cut(s) 346, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.