MD10G1294600.v1.1

Clavaminate synthase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005847
Physical Location & Seq
Forward (+)
38255115 .. 38256718
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1294600.v1.1.491

Sequence Viewer

Length: 996 bp
ATGACATTGACAATGGTGGAGATCCCAGAGCAAAAGCTTTACGACGGAAATCGATTCCCGGCAGTTATATCTCCGACTCCGACTCCGACTCCAGCTCCAGCAGCAGCTGCTGCTGCTACCCCTCGTCTCACAGAAACCATCCAAGCGAACAAACAGTACATACAGGACCAGCTCCGAAAATCGGGGGCCGTACTCTTCAGGGGGTTTCCTGTAAATACGGCCTCGGACTTCAACGACGTCGTGGAGGCCTTCGGCTACGAAGAGAATCCTTACGTTGGAACTTGGAACTGCTCCTCGGACCAAGATCGTGGGTCGGGTTTTTACCGCCAACGAGGCCCCACCAGATGTCAAAATTCCATTTCACCACGAAATGGCTTTGAGTACCCATCCAAACTGTTATTCTTTTGTGAAGTAGAGCCTGTAAGTGGGGGTGAAACTCCCATTGTTCTAAGCCACATTGTGTACGAGAGGATGAAAAACAAACACCCGCAATTTGTTCAAAAATTGGAGGACCATGGATTGATATATACCAAGATTATAGGCCACCACGACGACCCTTCTTCCGCGATAGGCCGTGGCTGGAAGGCCACATTTGCGACCGAAGATAAGAACATAGTTGAACAAAGGGTTTCTAAGGAGGGGTTTAGGTTGGAATGGTTGGATAATGGAGGAGTGAAAACAATCAAGGGACCGATCCTGGGCATCAAATGTGACAGCAGCACAGGGCAGCGCAAGACTTGGTTTAACAGCATCGTGATAGCATATACAGAGTGGAAAGGAGATGCAAGAAATGACCCTGAGAAGGTTGTCACATTCGGGGATGGCAGCCCGTTGGCAGCGGATATCATCTACGACTGCCAGAAAATTCTTGAAGTTGAAAGTGTGGCCATCCCTTGGCGCAAAGGTGATGTTTTGCTGCTGGACAATTTGGCCATTCTTCACTCCCGGAATCCATGTACTACACCTCGCAGAGTCCTTGCCGCCCTCTGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TauD

Protein Analysis

332

Amino Acids

36.86

Weight (kDa)

7.0

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TauD PF02668 42 - 326 2.5e-21 Taurine catabolism dioxygenase TauD, TfdA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AccB7I CCANNNNNTGG 2 cut(s) 371, 894
AccII CGCG 1 cut(s) 566
AciI CCGC 5 cut(s) 325, 488, 564, 839, 981
AclWI GGATC 2 cut(s) 16, 688
AcoI YGGCCR 2 cut(s) 885, 930
AcsI RAATTY 2 cut(s) 352, 864
AcuI CTGAAG 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 237
AdeI CACNNNGTG 1 cut(s) 460
AfaI GTAC 5 cut(s) 158, 192, 383, 464, 958
AfiI CCNNNNNNNGG 7 cut(s) 181, 275, 371, 425, 698, 802, 894
AgsI TTSAA 5 cut(s) 232, 500, 620, 872, 878
AjnI CCWGG 1 cut(s) 696
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 4 cut(s) 37, 95, 107, 172
AluI AGCT 4 cut(s) 37, 95, 107, 172
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 2 cut(s) 16, 688
AlwNI CAGNNNCTG 2 cut(s) 107, 110
AoxI GGCC 9 cut(s) 186, 219, 246, 334, 541, 571, 585, 885, 930
ApoI RAATTY 2 cut(s) 352, 864
AspLEI GCGC 2 cut(s) 732, 900
AspS9I GGNCC 6 cut(s) 166, 186, 298, 335, 511, 689
AsuC2I CCSGG 2 cut(s) 59, 946
AsuHPI GGTGA 3 cut(s) 354, 443, 917
AvaII GGWCC 4 cut(s) 166, 298, 511, 689
BalI TGGCCA 2 cut(s) 887, 932
BccI CCATC 4 cut(s) 146, 394, 815, 896
BceAI ACGGC 3 cut(s) 173, 234, 558
BciT130I CCWGG 1 cut(s) 698
BcnI CCSGG 2 cut(s) 59, 946
BcoDI GTCTC 1 cut(s) 131
BglI GCCNNNNNGGC 1 cut(s) 333
Bme1390I CCNGG 3 cut(s) 59, 698, 946
Bme18I GGWCC 4 cut(s) 166, 298, 511, 689
BmgT120I GGNCC 6 cut(s) 166, 186, 298, 335, 511, 689
BmiI GGNNCC 3 cut(s) 187, 337, 690
BmrFI CCNGG 3 cut(s) 59, 698, 946
BmsI GCATC 3 cut(s) 711, 759, 772
BpmI CTGGAG 2 cut(s) 75, 81
BpuMI CCSGG 2 cut(s) 59, 946
Bsa29I ATCGAT 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 6 cut(s) 222, 294, 514, 574, 697, 893
BsaXI ACNNNNNCTCC 6 cut(s) 67, 73, 79, 97, 103, 109
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 275, 371, 425, 698, 802, 894
BseBI CCWGG 1 cut(s) 698
BseCI ATCGAT 1 cut(s) 52
BseDI CCNNGG 6 cut(s) 222, 294, 514, 574, 697, 893
BseGI GGATG 5 cut(s) 138, 386, 477, 826, 888
BseLI CCNNNNNNNGG 7 cut(s) 181, 275, 371, 425, 698, 802, 894
BseMII CTCAG 1 cut(s) 789
BseRI GAGGAG 2 cut(s) 283, 684
Bsh1236I CGCG 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 600
BshFI GGCC 9 cut(s) 188, 221, 248, 336, 543, 573, 587, 887, 932
BshVI ATCGAT 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 600
BsiSI CCGG 2 cut(s) 59, 946
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 7 cut(s) 181, 275, 371, 425, 698, 802, 894
BsmAI GTCTC 1 cut(s) 131
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 9 cut(s) 188, 221, 248, 336, 543, 573, 587, 887, 932
Bsp143I GATC 3 cut(s) 21, 304, 693
Bsp19I CCATGG 1 cut(s) 514
BspACI CCGC 5 cut(s) 325, 488, 564, 839, 981
BspANI GGCC 9 cut(s) 188, 221, 248, 336, 543, 573, 587, 887, 932
BspCNI CTCAG 1 cut(s) 790
BspDI ATCGAT 1 cut(s) 52
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 3 cut(s) 187, 337, 690
BspPI GGATC 2 cut(s) 16, 688
BssECI CCNNGG 6 cut(s) 222, 294, 514, 574, 697, 893
BssMI GATC 3 cut(s) 21, 304, 693
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 514, 893
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 2 cut(s) 156, 396
Bst6I CTCTTC 2 cut(s) 200, 255
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 2 cut(s) 107, 110
BstDEI CTNAG 3 cut(s) 449, 633, 798
BstDSI CCRYGG 2 cut(s) 514, 574
BstF5I GGATG 5 cut(s) 138, 386, 477, 826, 888
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 2 cut(s) 732, 900
BstKTI GATC 3 cut(s) 24, 307, 696
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 21, 304, 693
BstMCI CGRYCG 1 cut(s) 600
BstMWI GCNNNNNNNGC 6 cut(s) 101, 107, 110, 113, 333, 593
BstNI CCWGG 1 cut(s) 698
BstSCI CCNGG 3 cut(s) 57, 696, 944
BstUI CGCG 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 52
BsuRI GGCC 9 cut(s) 188, 221, 248, 336, 543, 573, 587, 887, 932
BsuTUI ATCGAT 1 cut(s) 52
BtgI CCRYGG 2 cut(s) 514, 574
BtsCI GGATG 5 cut(s) 138, 386, 477, 826, 888
CaiI CAGNNNCTG 2 cut(s) 107, 110
CfoI GCGC 2 cut(s) 732, 900
Cfr13I GGNCC 6 cut(s) 166, 186, 298, 335, 511, 689
ClaI ATCGAT 1 cut(s) 52
Csp6I GTAC 5 cut(s) 157, 191, 382, 463, 957
CviAII CATG 2 cut(s) 515, 954
CviQI GTAC 5 cut(s) 157, 191, 382, 463, 957
DdeI CTNAG 3 cut(s) 449, 633, 798
DpnI GATC 3 cut(s) 23, 306, 695
DpnII GATC 3 cut(s) 21, 304, 693
DraIII CACNNNGTG 1 cut(s) 460
EaeI YGGCCR 2 cut(s) 885, 930
Eam1104I CTCTTC 2 cut(s) 200, 255
EarI CTCTTC 2 cut(s) 200, 255
Eco130I CCWWGG 2 cut(s) 514, 893
Eco147I AGGCCT 1 cut(s) 248
Eco32I GATATC 1 cut(s) 844
Eco47I GGWCC 4 cut(s) 166, 298, 511, 689
Eco57I CTGAAG 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 2 cut(s) 514, 893
ErhI CCWWGG 2 cut(s) 514, 893
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 2 cut(s) 518, 957
FaqI GGGAC 1 cut(s) 702
FatI CATG 2 cut(s) 514, 953
FauI CCCGC 1 cut(s) 495
FokI GGATG 5 cut(s) 125, 373, 484, 833, 875
GlaI GCGC 2 cut(s) 731, 899
GsuI CTGGAG 2 cut(s) 75, 81
HaeIII GGCC 9 cut(s) 188, 221, 248, 336, 543, 573, 587, 887, 932
HapII CCGG 2 cut(s) 59, 946
HhaI GCGC 2 cut(s) 732, 900
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 2 cut(s) 518, 957
Hin6I GCGC 2 cut(s) 730, 898
HinP1I GCGC 2 cut(s) 730, 898
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 7 cut(s) 54, 76, 82, 88, 265, 949, 972
HpaII CCGG 2 cut(s) 59, 946
HphI GGTGA 3 cut(s) 354, 443, 917
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 6 cut(s) 75, 81, 87, 176, 226, 298
Hpy188III TCNNGA 2 cut(s) 754, 869
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 4 cut(s) 47, 239, 242, 554
HpyAV CCTTC 4 cut(s) 259, 567, 577, 796
HpyCH4III ACNGT 2 cut(s) 156, 396
HpyCH4IV ACGT 2 cut(s) 237, 273
HpyCH4V TGCA 2 cut(s) 785, 990
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 107, 110, 113, 333, 593
HpyF3I CTNAG 3 cut(s) 449, 633, 798
HpySE526I ACGT 2 cut(s) 237, 273
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 2 cut(s) 518, 957
HspAI GCGC 2 cut(s) 730, 898
Kzo9I GATC 3 cut(s) 21, 304, 693
LmnI GCTCC 3 cut(s) 100, 177, 296
LweI GCATC 3 cut(s) 711, 759, 772
MaeII ACGT 2 cut(s) 237, 273
MaeIII GTNAC 2 cut(s) 710, 808
MalI GATC 3 cut(s) 23, 306, 695
MboI GATC 3 cut(s) 21, 304, 693
MboII GAAGA 5 cut(s) 187, 272, 552, 614, 929
MflI RGATCY 1 cut(s) 21
MlsI TGGCCA 2 cut(s) 887, 932
MluCI AATT 5 cut(s) 352, 491, 503, 864, 925
MluNI TGGCCA 2 cut(s) 887, 932
MlyI GAGTC 4 cut(s) 70, 76, 82, 981
MmeI TCCRAC 6 cut(s) 98, 104, 110, 256, 630, 639
Mox20I TGGCCA 2 cut(s) 887, 932
MscI TGGCCA 2 cut(s) 887, 932
MseI TTAA 1 cut(s) 744
Msp20I TGGCCA 2 cut(s) 887, 932
MspA1I CMGCKG 2 cut(s) 107, 839
MspI CCGG 2 cut(s) 59, 946
MspR9I CCNGG 3 cut(s) 59, 698, 946
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 6 cut(s) 101, 107, 110, 113, 333, 593
NciI CCSGG 2 cut(s) 59, 946
NcoI CCATGG 1 cut(s) 514
NdeII GATC 3 cut(s) 21, 304, 693
NlaIII CATG 2 cut(s) 518, 957
NlaIV GGNNCC 3 cut(s) 187, 337, 690
NmuCI GTSAC 2 cut(s) 710, 808
PceI AGGCCT 1 cut(s) 248
PfeI GAWTC 3 cut(s) 54, 265, 949
PflMI CCANNNNNTGG 2 cut(s) 371, 894
PfoI TCCNGGA 1 cut(s) 944
PleI GAGTC 4 cut(s) 70, 76, 82, 980
PpsI GAGTC 4 cut(s) 70, 76, 82, 980
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 3 cut(s) 187, 337, 690
PspPI GGNCC 6 cut(s) 166, 186, 298, 335, 511, 689
PsrI GAACNNNNNNTAC 2 cut(s) 140, 172
PstNI CAGNNNCTG 2 cut(s) 107, 110
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 5 cut(s) 158, 192, 383, 464, 958
RsaNI GTAC 5 cut(s) 157, 191, 382, 463, 957
SaqAI TTAA 1 cut(s) 744
Sau3AI GATC 3 cut(s) 21, 304, 693
Sau96I GGNCC 6 cut(s) 166, 186, 298, 335, 511, 689
SchI GAGTC 4 cut(s) 70, 76, 82, 981
ScrFI CCNGG 3 cut(s) 59, 698, 946
SfaNI GCATC 3 cut(s) 711, 759, 772
SinI GGWCC 4 cut(s) 166, 298, 511, 689
Sse9I AATT 5 cut(s) 352, 491, 503, 864, 925
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 5 cut(s) 325, 488, 564, 839, 981
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 3 cut(s) 57, 696, 944
StyI CCWWGG 2 cut(s) 514, 893
TaaI ACNGT 2 cut(s) 156, 396
TaiI ACGT 2 cut(s) 240, 276
TaqI TCGA 1 cut(s) 52
TaqII GACCGA 2 cut(s) 614, 706
TasI AATT 5 cut(s) 352, 491, 503, 864, 925
TatI WGTACW 2 cut(s) 156, 956
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 3 cut(s) 54, 265, 949
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TseFI GTSAC 2 cut(s) 710, 808
Tsp45I GTSAC 2 cut(s) 710, 808
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 60
Van91I CCANNNNNTGG 2 cut(s) 371, 894
VpaK11BI GGWCC 4 cut(s) 166, 298, 511, 689
XapI RAATTY 2 cut(s) 352, 864
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.