MD10G1294700.v1.1

Clavaminate synthase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005847
Physical Location & Seq
Forward (+)
38258276 .. 38260009
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1294700.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCATTGACAATGGTGGAGATCCCAGAGCAAAAGCTTTACGACGGAAATCGATTCCCGGCAGTTATATCTCCGACTCCGACTCCAGCAGCAGCTGCTACTCCTCGTCTCACAGAAACCATCCAAGCGAACAAACAGTACATACAGGACCAGCTCCTAAAATCCGGGGCCGTACTCTTCAGGGGGTTTCCTGTAAATACAGCCTCGGACTTCAACGACGTCGTGGAGGCCTTCGGCTACGAAGAGAATCCTTACGTTGGAACTGCTCCTCGGACAAAGATCGTGGGTCGGGTTTTCACCGCCAACGAGGCCCCGCCCGACCACAAGATTCCATTTCACCACGAAATGGTTCTGTATCCAGAGTTCCCATCCAAGCTGTTATTTTTCTGTGAAATAGAGCCTGTAAGTGGGGGCGAAACTCCCATTGTTCTAAGCCACATTGTGTACGAGAGGATGAAAGACAAGCACCCGCAATTTGTCCAAAAATTGGAGGAACATGGCTTGATATATACCAAGGTTTTAGGCCAAGATTACGACCCTTCTTCTGCGATGGGTCGTGGCTGGAAGTCCACATTTGGGACCGAAGACAAGAACATAGCTGAACAGAGGGCTGCTAAGCTGGGGTATGTGTTGGAATGGTTGGATGACGGAGTGAAAACAATCAGGGGACCGATCCCGGGCATCAAATGTGACAGCAGCACAAGGCAGCGCAAGGTTTGGTTTAACAACATCATGGCAGTATATTTAGGATGGAAAGATGATGCAAGAAATAGTGACCCTGAGAAGGCTCTCGCTTTCGGGGATGGCAGCCCCTTGGCTGCAGATGTCATCTACGACTGCCAGAAAATTCTTGAAGATGAAAGTGTGGCCATCCCTTGGCGCAAAGGTGATGTTTTGCTGCTCGATAATATGGCCGTCCTTCACTCCCGGAATCCATGCACTACACCTCGCAGAGTCCTAGCCTCCCTCTGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TauD

Protein Analysis

326

Amino Acids

36.34

Weight (kDa)

5.86

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TauD PF02668 39 - 320 2.3e-31 Taurine catabolism dioxygenase TauD, TfdA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
AccB7I CCANNNNNTGG 3 cut(s) 346, 487, 876
AciI CCGC 3 cut(s) 300, 314, 470
AclWI GGATC 2 cut(s) 16, 667
AcoI YGGCCR 2 cut(s) 867, 912
AcsI RAATTY 1 cut(s) 846
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 3 cut(s) 140, 174, 446
AfiI CCNNNNNNNGG 8 cut(s) 257, 346, 407, 487, 576, 677, 784, 876
AgsI TTSAA 2 cut(s) 214, 854
AluBI AGCT 6 cut(s) 37, 95, 154, 376, 599, 619
AluI AGCT 6 cut(s) 37, 95, 154, 376, 599, 619
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 2 cut(s) 16, 667
AlwNI CAGNNNCTG 1 cut(s) 95
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 6 cut(s) 168, 228, 309, 523, 867, 912
ApeKI GCWGC 9 cut(s) 89, 92, 95, 611, 696, 706, 807, 818, 898
ApoI RAATTY 1 cut(s) 846
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 2 cut(s) 711, 882
AspS9I GGNCC 5 cut(s) 148, 168, 310, 579, 668
AsuC2I CCSGG 5 cut(s) 59, 166, 677, 678, 928
AsuHPI GGTGA 3 cut(s) 289, 329, 899
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 3 cut(s) 148, 579, 668
BalI TGGCCA 1 cut(s) 869
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 9 cut(s) 82, 101, 104, 598, 708, 718, 805, 819, 885
BccI CCATC 6 cut(s) 128, 376, 544, 744, 797, 878
BceAI ACGGC 2 cut(s) 155, 899
BciVI GTATCC 1 cut(s) 366
BcnI CCSGG 5 cut(s) 59, 166, 677, 678, 928
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 1 cut(s) 819
BfuI GTATCC 1 cut(s) 366
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 9 cut(s) 90, 93, 96, 612, 697, 707, 808, 819, 899
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 9 cut(s) 91, 94, 97, 613, 698, 708, 809, 820, 900
Bme1390I CCNGG 5 cut(s) 59, 166, 677, 678, 928
Bme18I GGWCC 3 cut(s) 148, 579, 668
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 5 cut(s) 148, 168, 310, 579, 668
BmiI GGNNCC 4 cut(s) 169, 312, 580, 669
BmrFI CCNGG 5 cut(s) 59, 166, 677, 678, 928
BmsI GCATC 2 cut(s) 690, 751
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 69
Bpu1102I GCTNAGC 1 cut(s) 615
BpuMI CCSGG 5 cut(s) 59, 166, 677, 678, 928
Bsa29I ATCGAT 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 7 cut(s) 165, 204, 269, 513, 676, 813, 875
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 8 cut(s) 257, 346, 407, 487, 576, 677, 784, 876
BseCI ATCGAT 1 cut(s) 52
BseDI CCNNGG 7 cut(s) 165, 204, 269, 513, 676, 813, 875
BseGI GGATG 7 cut(s) 120, 368, 459, 649, 755, 808, 870
BseLI CCNNNNNNNGG 8 cut(s) 257, 346, 407, 487, 576, 677, 784, 876
BseMII CTCAG 1 cut(s) 771
BseRI GAGGAG 2 cut(s) 93, 258
BseXI GCAGC 9 cut(s) 82, 101, 104, 598, 708, 718, 805, 819, 885
BseYI CCCAGC 1 cut(s) 619
BshFI GGCC 6 cut(s) 170, 230, 311, 525, 869, 914
BshVI ATCGAT 1 cut(s) 52
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 4 cut(s) 59, 165, 677, 928
BslFI GGGAC 2 cut(s) 592, 681
BslI CCNNNNNNNGG 8 cut(s) 257, 346, 407, 487, 576, 677, 784, 876
BsmAI GTCTC 1 cut(s) 113
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 592, 681
BsnI GGCC 6 cut(s) 170, 230, 311, 525, 869, 914
BsoBI CYCGRG 1 cut(s) 676
Bsp143I GATC 3 cut(s) 21, 279, 672
Bsp1720I GCTNAGC 1 cut(s) 615
BspACI CCGC 3 cut(s) 300, 314, 470
BspANI GGCC 6 cut(s) 170, 230, 311, 525, 869, 914
BspCNI CTCAG 1 cut(s) 772
BspDI ATCGAT 1 cut(s) 52
BspLI GGNNCC 4 cut(s) 169, 312, 580, 669
BspMAI CTGCAG 1 cut(s) 823
BspPI GGATC 2 cut(s) 16, 667
BssECI CCNNGG 7 cut(s) 165, 204, 269, 513, 676, 813, 875
BssMI GATC 3 cut(s) 21, 279, 672
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 513, 813, 875
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 182, 237
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 95
BstDEI CTNAG 3 cut(s) 431, 615, 780
BstF5I GGATG 7 cut(s) 120, 368, 459, 649, 755, 808, 870
BstHHI GCGC 2 cut(s) 711, 882
BstKTI GATC 3 cut(s) 24, 282, 675
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 3 cut(s) 21, 279, 672
BstMWI GCNNNNNNNGC 2 cut(s) 95, 308
BstSCI CCNGG 5 cut(s) 57, 164, 675, 676, 926
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 9 cut(s) 82, 101, 104, 598, 708, 718, 805, 819, 885
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 52
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 6 cut(s) 170, 230, 311, 525, 869, 914
BsuTUI ATCGAT 1 cut(s) 52
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 7 cut(s) 120, 368, 459, 649, 755, 808, 870
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 2 cut(s) 711, 882
Cfr13I GGNCC 5 cut(s) 148, 168, 310, 579, 668
Cfr9I CCCGGG 1 cut(s) 676
ClaI ATCGAT 1 cut(s) 52
Csp6I GTAC 3 cut(s) 139, 173, 445
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 3 cut(s) 497, 733, 936
CviQI GTAC 3 cut(s) 139, 173, 445
DdeI CTNAG 3 cut(s) 431, 615, 780
DpnI GATC 3 cut(s) 23, 281, 674
DpnII GATC 3 cut(s) 21, 279, 672
DraIII CACNNNGTG 1 cut(s) 442
EaeI YGGCCR 2 cut(s) 867, 912
Eam1104I CTCTTC 2 cut(s) 182, 237
EarI CTCTTC 2 cut(s) 182, 237
Eco130I CCWWGG 3 cut(s) 513, 813, 875
Eco147I AGGCCT 1 cut(s) 230
Eco47I GGWCC 3 cut(s) 148, 579, 668
Eco57I CTGAAG 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 310
EcoT14I CCWWGG 3 cut(s) 513, 813, 875
ErhI CCWWGG 3 cut(s) 513, 813, 875
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 3 cut(s) 500, 736, 939
FaqI GGGAC 2 cut(s) 592, 681
FatI CATG 3 cut(s) 496, 732, 935
FauI CCCGC 2 cut(s) 321, 477
Fnu4HI GCNGC 9 cut(s) 90, 93, 96, 612, 697, 707, 808, 819, 899
FokI GGATG 7 cut(s) 107, 355, 466, 656, 762, 815, 857
Fsp4HI GCNGC 9 cut(s) 90, 93, 96, 612, 697, 707, 808, 819, 899
FspBI CTAG 1 cut(s) 959
GlaI GCGC 2 cut(s) 710, 881
GluI GCNGC 9 cut(s) 90, 93, 96, 612, 697, 707, 808, 819, 899
GsaI CCCAGC 1 cut(s) 623
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 6 cut(s) 170, 230, 311, 525, 869, 914
HapII CCGG 4 cut(s) 59, 165, 677, 928
HhaI GCGC 2 cut(s) 711, 882
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 3 cut(s) 500, 736, 939
Hin6I GCGC 2 cut(s) 709, 880
HinP1I GCGC 2 cut(s) 709, 880
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 7 cut(s) 54, 76, 82, 247, 328, 931, 954
HpaII CCGG 4 cut(s) 59, 165, 677, 928
HphI GGTGA 3 cut(s) 289, 329, 899
Hpy166II GTNNAC 2 cut(s) 445, 570
Hpy188I TCNGA 4 cut(s) 75, 81, 208, 273
Hpy188III TCNNGA 2 cut(s) 359, 851
Hpy8I GTNNAC 2 cut(s) 445, 570
Hpy99I CGWCG 3 cut(s) 47, 221, 224
HpyAV CCTTC 4 cut(s) 241, 549, 778, 929
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 2 cut(s) 219, 255
HpyCH4V TGCA 4 cut(s) 764, 821, 939, 972
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 308
HpyF3I CTNAG 3 cut(s) 431, 615, 780
HpySE526I ACGT 2 cut(s) 219, 255
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 3 cut(s) 500, 736, 939
HspAI GCGC 2 cut(s) 709, 880
Kzo9I GATC 3 cut(s) 21, 279, 672
LmnI GCTCC 2 cut(s) 159, 271
Lsp1109I GCAGC 9 cut(s) 82, 101, 104, 598, 708, 718, 805, 819, 885
LweI GCATC 2 cut(s) 690, 751
MaeI CTAG 1 cut(s) 959
MaeII ACGT 2 cut(s) 219, 255
MaeIII GTNAC 2 cut(s) 689, 773
MalI GATC 3 cut(s) 23, 281, 674
MboI GATC 3 cut(s) 21, 279, 672
MboII GAAGA 5 cut(s) 169, 254, 534, 596, 866
MflI RGATCY 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 869
MluCI AATT 3 cut(s) 473, 485, 846
MluNI TGGCCA 1 cut(s) 869
MlyI GAGTC 3 cut(s) 70, 76, 963
MmeI TCCRAC 5 cut(s) 98, 104, 238, 612, 621
Mox20I TGGCCA 1 cut(s) 869
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 869
MseI TTAA 1 cut(s) 723
Msp20I TGGCCA 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 4 cut(s) 59, 165, 677, 928
MspR9I CCNGG 5 cut(s) 59, 166, 677, 678, 928
MwoI GCNNNNNNNGC 2 cut(s) 95, 308
NciI CCSGG 5 cut(s) 59, 166, 677, 678, 928
NdeII GATC 3 cut(s) 21, 279, 672
NlaIII CATG 3 cut(s) 500, 736, 939
NlaIV GGNNCC 4 cut(s) 169, 312, 580, 669
NmuCI GTSAC 2 cut(s) 689, 773
PceI AGGCCT 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 54, 247, 328, 931
PflMI CCANNNNNTGG 3 cut(s) 346, 487, 876
PfoI TCCNGGA 1 cut(s) 926
PkrI GCNGC 9 cut(s) 91, 94, 97, 613, 698, 708, 809, 820, 900
PleI GAGTC 3 cut(s) 70, 76, 962
PpsI GAGTC 3 cut(s) 70, 76, 962
PspFI CCCAGC 1 cut(s) 619
PspN4I GGNNCC 4 cut(s) 169, 312, 580, 669
PspPI GGNCC 5 cut(s) 148, 168, 310, 579, 668
PsrI GAACNNNNNNTAC 4 cut(s) 122, 154, 347, 379
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 3 cut(s) 140, 174, 446
RsaNI GTAC 3 cut(s) 139, 173, 445
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 9 cut(s) 90, 93, 96, 612, 697, 707, 808, 819, 899
Sau3AI GATC 3 cut(s) 21, 279, 672
Sau96I GGNCC 5 cut(s) 148, 168, 310, 579, 668
SchI GAGTC 3 cut(s) 70, 76, 963
ScrFI CCNGG 5 cut(s) 59, 166, 677, 678, 928
SfaNI GCATC 2 cut(s) 690, 751
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 3 cut(s) 148, 579, 668
SmaI CCCGGG 1 cut(s) 678
Sse9I AATT 3 cut(s) 473, 485, 846
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 3 cut(s) 300, 314, 470
SspMI CTAG 1 cut(s) 959
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 5 cut(s) 57, 164, 675, 676, 926
StyI CCWWGG 3 cut(s) 513, 813, 875
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 2 cut(s) 222, 258
TaqI TCGA 2 cut(s) 52, 903
TaqII GACCGA 2 cut(s) 596, 685
TasI AATT 3 cut(s) 473, 485, 846
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 4 cut(s) 54, 247, 328, 931
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TseFI GTSAC 2 cut(s) 689, 773
TseI GCWGC 9 cut(s) 89, 92, 95, 611, 696, 706, 807, 818, 898
Tsp45I GTSAC 2 cut(s) 689, 773
TspDTI ATGAA 2 cut(s) 470, 873
TspGWI ACGGA 2 cut(s) 60, 663
TspMI CCCGGG 1 cut(s) 676
Van91I CCANNNNNTGG 3 cut(s) 346, 487, 876
VpaK11BI GGWCC 3 cut(s) 148, 579, 668
XapI RAATTY 1 cut(s) 846
XmaI CCCGGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 959
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.