MD11G1153900.v1.1

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001514
Physical Location & Seq
Reverse (-)
14751537 .. 14756980
5444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1153900.v1.1.491

Sequence Viewer

Length: 1782 bp
ATGGAGACTCTCTGCAGCCTGACTCCTTCACCCTCCTCACCTCTGTTCCGTACAAACGTACGGCAGCATAGTTCATCATCAACATCCTTCCCACACCTCATAATAAACCAATCACCATGCATCACCACTGCTATACTAACAAATCCAGCTGCTGAAGTAATCTCCATCAAACCTTCCAAACTACCACTCGGTCCCTTCAAAACGACACCGCAGGAGTTGAAGATAGTGGCCCCGGAGACATTACAGTACGAGAGTGGGTTTCTTGGAGCAGTGCCTGATAAATTAGTGGAAGGAGAAGGAAGTAGTGATGATATTCACGATGCCATGAGTTACTTGACAAAGATTTTGACCTCTAAAGCTTATGATGTTGCCATTGAGTCTCCATTGGACTATGCTTCCAAGCTCTCTGAGAAGCTTCATGTTAATATTTGGCTCAAGAGAGAGGACCTACAACCTATTTTTTCCTTTAAAATTCGAGGAGCTTACAATATGATGGCAAAGCTTTCCACGAAACAATTGGCAATGGGTGTCATTTGTTCATCAGCAGGGAATCATGCTCAAGGAGTTGCACTATCAGCACAAAAACTTGGTTGCACTGCTCTGATTGTCATGCCCATTACAACTCCTGAAATCAAATGGAAAACAGTGGAGAAGTTAGGTGGACAAGTAGTGCTTGTGGGTGACTCATATGATGAAGCCCAAGCATATGCACAACAAAGGGCCAAAGATGAAGGCTTAATATTTGTGCACCCTTTTGACCACCCAGATGTCATAGCTGGCCAAGGAACTATAGGAATGGAGATTCTGCGTCAAATTCAAAGTCCATTGCATGCAAGAGAGTTCGCCCCGAGGTTTGTCAAGATAATTGGGGTAGAGGTATCCGATGCAAACGCAATGGCACTATCCATACATCATGGTCAGAGAATTATGTTGGTGAAAGTGGGAGGATTTGCAGATGGAATAGCAGTAAAAAAGGTAGGGGAAGAAACGTTTCGGTTATGCAGAGAACTCATTGATGGAGTAGTTCTTGTTAGTCGTGATGCCGTTTGTGCATCAATAAAAGATATGTTTGAGGAAAAACGGAGCATACTTGAACCGGCAGGAGCTGTAGCCATCGCTGGAGCAGAAGCATATTGCAAATATTATGGACTGAAGGGTGAAAATGTTGTTGCTATAAGTAGTGGAGCTAACATGAACTTTGACAGACTAAGATTGGTATCTCAACTAGCCGATGTTGGTAGGAGACGCGAAGTTTTCCTTGCAACATTTGTTCCAGAAAAGCGTGGAAGCTTCAAACAGTTTTGTGAACTGGTTGGTCCAATGAATATTCTTGAGATAAACTACAGATATGATGATAAAAAAGAAAGTGGCCTTATTCTGTACAGTGTTGGCTTTCAAACTGTTTTGGAGTTGGAAGAAATGTTGGATCGGATGAAGTCGGCTCAACTACTGACGATTAACCTTACGAATAAGGAACTAGTTAAAGATCATATGCAATTTTTGGTGGGAGGTCGATCGTCCTCTATCGAAAATGAGATCCTTTGTCAGTTTTCCATTCCAGCGAGGCCAGGTGCTTTGTTGAAGTTCTTAGATGCTTTCAGTCCCCTTTGGAACATAAGCTTGTTCCATTACAGTGGACAGGGTGAAAGTGGTGCGGATGCGTTGGTCGGTTTACATGTTGCAAGCTCTGAGATAAACAAGTTCAAGGCTTTGGCTGATGCTCTAGGGTTTGATTATAAATTTGAGACAAACAATGAGACCTTACAATTGTTGTTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

594

Amino Acids

65.06

Weight (kDa)

6.1

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 126 - 395 1.1e-61 Pyridoxal-phosphate dependent enzyme
Thr_dehydrat_C PF00585 410 - 497 1.8e-15 C-terminal regulatory domain of Threonine dehydratase
Thr_dehydrat_C PF00585 504 - 592 7.5e-18 C-terminal regulatory domain of Threonine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1737
AccII CGCG 1 cut(s) 1248
AciI CCGC 2 cut(s) 209, 1655
AclI AACGTT 1 cut(s) 989
AclWI GGATC 2 cut(s) 1434, 1531
AcoI YGGCCR 1 cut(s) 778
AcsI RAATTY 3 cut(s) 471, 813, 1739
AcuI CTGAAG 2 cut(s) 174, 1172
AfaI GTAC 4 cut(s) 52, 60, 248, 1382
AflIII ACRYGT 1 cut(s) 1675
AgsI TTSAA 8 cut(s) 199, 220, 818, 1094, 1294, 1397, 1582, 1705
AhlI ACTAGT 1 cut(s) 1477
AjnI CCWGG 1 cut(s) 1567
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 5 cut(s) 230, 384, 1237, 1739, 1751
Alw44I GTGCAC 1 cut(s) 746
AlwI GGATC 2 cut(s) 1434, 1531
AlwNI CAGNNNCTG 3 cut(s) 152, 275, 1106
Ama87I CYCGRG 1 cut(s) 847
AoxI GGCC 5 cut(s) 228, 720, 778, 1369, 1565
ApaLI GTGCAC 1 cut(s) 746
ApeKI GCWGC 3 cut(s) 15, 64, 149
ApoI RAATTY 3 cut(s) 471, 813, 1739
ArsI GACNNNNNNTTYG 4 cut(s) 328, 360, 805, 837
Asp700I GAANNNNTTC 1 cut(s) 990
AspS9I GGNCC 5 cut(s) 191, 229, 445, 720, 1316
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 8 cut(s) 21, 30, 105, 115, 692, 946, 1169, 1655
AvaI CYCGRG 1 cut(s) 847
AvaII GGWCC 3 cut(s) 191, 445, 1316
BaeGI GKGCMC 1 cut(s) 750
BalI TGGCCA 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 3 cut(s) 27, 76, 136
BccI CCATC 5 cut(s) 173, 487, 950, 1010, 1121
BceAI ACGGC 2 cut(s) 77, 1028
BciT130I CCWGG 1 cut(s) 1569
BciVI GTATCC 1 cut(s) 889
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 5 cut(s) 230, 384, 1237, 1739, 1751
BcuI ACTAGT 1 cut(s) 1477
BfaI CTAG 3 cut(s) 1226, 1478, 1724
BfmI CTRYAG 4 cut(s) 13, 789, 1107, 1342
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 3 cut(s) 16, 65, 150
BlsI GCNGC 3 cut(s) 17, 66, 151
Bme1390I CCNGG 2 cut(s) 233, 1569
Bme18I GGWCC 3 cut(s) 191, 445, 1316
BmeT110I CYCGRG 1 cut(s) 847
BmgT120I GGNCC 5 cut(s) 191, 229, 445, 720, 1316
BmiI GGNNCC 2 cut(s) 193, 231
BmrFI CCNGG 2 cut(s) 233, 1569
BmsI GCATC 8 cut(s) 129, 310, 874, 1030, 1061, 1582, 1648, 1708
BpmI CTGGAG 1 cut(s) 1140
BpuEI CTTGAG 3 cut(s) 419, 543, 1352
BpuMI CCSGG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 1216
BsaI GGTCTC 1 cut(s) 1751
BsaJI CCNNGG 3 cut(s) 231, 781, 848
Bse118I RCCGGY 1 cut(s) 1096
Bse1I ACTGG 1 cut(s) 1314
Bse3DI GCAATG 3 cut(s) 528, 824, 900
Bse8I GATNNNNATC 1 cut(s) 1216
BseBI CCWGG 1 cut(s) 1569
BseDI CCNNGG 3 cut(s) 231, 781, 848
BseGI GGATG 3 cut(s) 83, 1437, 1663
BseJI GATNNNNATC 1 cut(s) 1216
BseMI GCAATG 3 cut(s) 528, 824, 900
BseMII CTCAG 2 cut(s) 399, 1680
BseNI ACTGG 1 cut(s) 1314
BseRI GAGGAG 2 cut(s) 25, 492
BseSI GKGCMC 1 cut(s) 750
BseXI GCAGC 3 cut(s) 27, 76, 136
Bsh1236I CGCG 1 cut(s) 1248
Bsh1285I CGRYCG 1 cut(s) 1517
BshFI GGCC 5 cut(s) 230, 722, 780, 1371, 1567
BsiEI CGRYCG 1 cut(s) 1517
BsiHKAI GWGCWC 1 cut(s) 750
BsiHKCI CYCGRG 1 cut(s) 847
BsiSI CCGG 2 cut(s) 233, 1097
BsiWI CGTACG 1 cut(s) 58
BslFI GGGAC 2 cut(s) 177, 1587
BsmAI GTCTC 5 cut(s) 230, 384, 1237, 1739, 1751
BsmBI CGTCTC 1 cut(s) 1237
BsmFI GGGAC 2 cut(s) 177, 1587
BsnI GGCC 5 cut(s) 230, 722, 780, 1371, 1567
Bso31I GGTCTC 1 cut(s) 1751
BsoBI CYCGRG 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 750
Bsp1407I TGTACA 1 cut(s) 1380
Bsp143I GATC 4 cut(s) 1426, 1486, 1514, 1536
BspACI CCGC 2 cut(s) 209, 1655
BspANI GGCC 5 cut(s) 230, 722, 780, 1371, 1567
BspCNI CTCAG 2 cut(s) 400, 1681
BspFNI CGCG 1 cut(s) 1248
BspLI GGNNCC 2 cut(s) 193, 231
BspMAI CTGCAG 1 cut(s) 17
BspPI GGATC 2 cut(s) 1434, 1531
BspTNI GGTCTC 1 cut(s) 1751
BsrDI GCAATG 3 cut(s) 528, 824, 900
BsrFI RCCGGY 1 cut(s) 1096
BsrGI TGTACA 1 cut(s) 1380
BsrI ACTGG 1 cut(s) 1314
BssAI RCCGGY 1 cut(s) 1096
BssECI CCNNGG 3 cut(s) 231, 781, 848
BssMI GATC 4 cut(s) 1426, 1486, 1514, 1536
BssT1I CCWWGG 1 cut(s) 781
Bst2UI CCWGG 1 cut(s) 1569
Bst4CI ACNGT 6 cut(s) 246, 646, 1299, 1385, 1402, 1634
BstAUI TGTACA 1 cut(s) 1380
BstC8I GCNNGC 3 cut(s) 778, 831, 1684
BstDEI CTNAG 4 cut(s) 408, 1208, 1588, 1689
BstF5I GGATG 3 cut(s) 83, 1437, 1663
BstFNI CGCG 1 cut(s) 1248
BstKTI GATC 4 cut(s) 1429, 1489, 1517, 1539
BstMAI GTCTC 5 cut(s) 230, 384, 1237, 1739, 1751
BstMBI GATC 4 cut(s) 1426, 1486, 1514, 1536
BstMCI CGRYCG 1 cut(s) 1517
BstMWI GCNNNNNNNGC 2 cut(s) 575, 1049
BstNI CCWGG 1 cut(s) 1569
BstNSI RCATGY 2 cut(s) 833, 1679
BstSCI CCNGG 2 cut(s) 231, 1567
BstSFI CTRYAG 4 cut(s) 13, 789, 1107, 1342
BstSLI GKGCMC 1 cut(s) 750
BstUI CGCG 1 cut(s) 1248
BstV1I GCAGC 3 cut(s) 27, 76, 136
BstX2I RGATCY 1 cut(s) 1536
BstXI CCANNNNNNTGG 1 cut(s) 1634
BstYI RGATCY 1 cut(s) 1536
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 5 cut(s) 230, 722, 780, 1371, 1567
BtgZI GCGATG 1 cut(s) 1099
BtsCI GGATG 3 cut(s) 83, 1437, 1663
BtsI GCAGTG 3 cut(s) 126, 276, 594
BtsIMutI CAGTG 6 cut(s) 126, 276, 594, 651, 1390, 1639
Cac8I GCNNGC 3 cut(s) 778, 831, 1684
CaiI CAGNNNCTG 3 cut(s) 152, 275, 1106
Cfr10I RCCGGY 1 cut(s) 1096
Cfr13I GGNCC 5 cut(s) 191, 229, 445, 720, 1316
CseI GACGC 2 cut(s) 797, 1254
Csp6I GTAC 4 cut(s) 51, 59, 247, 1381
CviAII CATG 9 cut(s) 117, 325, 419, 554, 610, 830, 914, 1192, 1676
CviQI GTAC 4 cut(s) 51, 59, 247, 1381
DdeI CTNAG 4 cut(s) 408, 1208, 1588, 1689
DpnI GATC 4 cut(s) 1428, 1488, 1516, 1538
DpnII GATC 4 cut(s) 1426, 1486, 1514, 1536
DraI TTTAAA 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 778
Eco130I CCWWGG 1 cut(s) 781
Eco31I GGTCTC 1 cut(s) 1751
Eco47I GGWCC 3 cut(s) 191, 445, 1316
Eco57I CTGAAG 2 cut(s) 174, 1172
Eco88I CYCGRG 1 cut(s) 847
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 1567
EcoT14I CCWWGG 1 cut(s) 781
EcoT22I ATGCAT 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 781
Esp3I CGTCTC 1 cut(s) 1237
FaeI CATG 9 cut(s) 120, 328, 422, 557, 613, 833, 917, 1195, 1679
FalI AAGNNNNNCTT 4 cut(s) 657, 689, 1242, 1274
FaqI GGGAC 2 cut(s) 177, 1587
FatI CATG 9 cut(s) 116, 324, 418, 553, 609, 829, 913, 1191, 1675
FauNDI CATATG 3 cut(s) 688, 706, 1491
Fnu4HI GCNGC 3 cut(s) 16, 65, 150
FokI GGATG 3 cut(s) 70, 1444, 1670
Fsp4HI GCNGC 3 cut(s) 16, 65, 150
FspBI CTAG 3 cut(s) 1226, 1478, 1724
GluI GCNGC 3 cut(s) 16, 65, 150
GsuI CTGGAG 1 cut(s) 1140
HaeIII GGCC 5 cut(s) 230, 722, 780, 1371, 1567
HapII CCGG 2 cut(s) 233, 1097
HgaI GACGC 2 cut(s) 797, 1254
Hin1II CATG 9 cut(s) 120, 328, 422, 557, 613, 833, 917, 1195, 1679
HindIII AAGCTT 5 cut(s) 357, 413, 500, 1288, 1618
HinfI GANTC 6 cut(s) 7, 22, 377, 550, 683, 802
HpaII CCGG 2 cut(s) 233, 1097
HphI GGTGA 8 cut(s) 21, 30, 105, 115, 692, 946, 1169, 1655
Hpy166II GTNNAC 5 cut(s) 662, 748, 1307, 1637, 1673
Hpy188I TCNGA 6 cut(s) 409, 603, 883, 921, 1431, 1690
Hpy188III TCNNGA 7 cut(s) 317, 436, 626, 859, 1037, 1274, 1331
Hpy8I GTNNAC 5 cut(s) 662, 748, 1307, 1637, 1673
HpyAV CCTTC 8 cut(s) 36, 97, 183, 205, 284, 290, 725, 1147
HpyCH4III ACNGT 6 cut(s) 246, 646, 1299, 1385, 1402, 1634
HpyCH4IV ACGT 2 cut(s) 57, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 1049
HpyF3I CTNAG 4 cut(s) 408, 1208, 1588, 1689
HpySE526I ACGT 2 cut(s) 57, 989
Hsp92II CATG 9 cut(s) 120, 328, 422, 557, 613, 833, 917, 1195, 1679
Kzo9I GATC 4 cut(s) 1426, 1486, 1514, 1536
LmnI GCTCC 6 cut(s) 266, 479, 1083, 1103, 1121, 1184
Lsp1109I GCAGC 3 cut(s) 27, 76, 136
LweI GCATC 8 cut(s) 129, 310, 874, 1030, 1061, 1582, 1648, 1708
MaeI CTAG 3 cut(s) 1226, 1478, 1724
MaeII ACGT 2 cut(s) 57, 989
MaeIII GTNAC 2 cut(s) 329, 680
MalI GATC 4 cut(s) 1428, 1488, 1516, 1538
MboI GATC 4 cut(s) 1426, 1486, 1514, 1536
MboII GAAGA 3 cut(s) 232, 995, 1427
MfeI CAATTG 2 cut(s) 515, 1766
MflI RGATCY 1 cut(s) 1536
MhlI GDGCHC 1 cut(s) 750
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 9 cut(s) 281, 471, 515, 813, 864, 924, 1496, 1739, 1766
MluNI TGGCCA 1 cut(s) 780
MlyI GAGTC 3 cut(s) 16, 386, 677
MmeI TCCRAC 2 cut(s) 1392, 1404
Mox20I TGGCCA 1 cut(s) 780
Mph1103I ATGCAT 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 990
MscI TGGCCA 1 cut(s) 780
MseI TTAA 5 cut(s) 423, 468, 737, 1458, 1482
MslI CAYNNNNRTG 2 cut(s) 765, 1632
Msp20I TGGCCA 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 233, 1097
MspR9I CCNGG 2 cut(s) 233, 1569
MunI CAATTG 2 cut(s) 515, 1766
MvaI CCWGG 1 cut(s) 1569
MvnI CGCG 1 cut(s) 1248
MwoI GCNNNNNNNGC 2 cut(s) 575, 1049
NciI CCSGG 1 cut(s) 233
NdeI CATATG 3 cut(s) 688, 706, 1491
NdeII GATC 4 cut(s) 1426, 1486, 1514, 1536
NlaIII CATG 9 cut(s) 120, 328, 422, 557, 613, 833, 917, 1195, 1679
NlaIV GGNNCC 2 cut(s) 193, 231
NmuCI GTSAC 1 cut(s) 680
NsiI ATGCAT 1 cut(s) 122
NspI RCATGY 2 cut(s) 833, 1679
PaeI GCATGC 1 cut(s) 833
PciI ACATGT 1 cut(s) 1675
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 2 cut(s) 550, 802
Pfl23II CGTACG 1 cut(s) 58
PkrI GCNGC 3 cut(s) 17, 66, 151
Ple19I CGATCG 1 cut(s) 1517
PleI GAGTC 3 cut(s) 16, 385, 677
PpsI GAGTC 3 cut(s) 16, 385, 677
PpuMI RGGWCCY 1 cut(s) 445
PscI ACATGT 1 cut(s) 1675
PsiI TTATAA 1 cut(s) 1737
Psp1406I AACGTT 1 cut(s) 989
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 1567
PspGI CCWGG 1 cut(s) 1567
PspLI CGTACG 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 193, 231
PspPI GGNCC 5 cut(s) 191, 229, 445, 720, 1316
PspPPI RGGWCCY 1 cut(s) 445
PstI CTGCAG 1 cut(s) 17
PstNI CAGNNNCTG 3 cut(s) 152, 275, 1106
PsuI RGATCY 1 cut(s) 1536
PvuI CGATCG 1 cut(s) 1517
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 4 cut(s) 52, 60, 248, 1382
RsaNI GTAC 4 cut(s) 51, 59, 247, 1381
RseI CAYNNNNRTG 2 cut(s) 765, 1632
SaqAI TTAA 5 cut(s) 423, 468, 737, 1458, 1482
SatI GCNGC 3 cut(s) 16, 65, 150
Sau3AI GATC 4 cut(s) 1426, 1486, 1514, 1536
Sau96I GGNCC 5 cut(s) 191, 229, 445, 720, 1316
SchI GAGTC 3 cut(s) 16, 386, 677
ScrFI CCNGG 2 cut(s) 233, 1569
SduI GDGCHC 1 cut(s) 750
SfaNI GCATC 8 cut(s) 129, 310, 874, 1030, 1061, 1582, 1648, 1708
SfcI CTRYAG 4 cut(s) 13, 789, 1107, 1342
SinI GGWCC 3 cut(s) 191, 445, 1316
SmiMI CAYNNNNRTG 2 cut(s) 765, 1632
SmlI CTYRAG 3 cut(s) 434, 558, 1331
SmoI CTYRAG 3 cut(s) 434, 558, 1331
SpeI ACTAGT 1 cut(s) 1477
SphI GCATGC 1 cut(s) 833
Sse9I AATT 9 cut(s) 281, 471, 515, 813, 864, 924, 1496, 1739, 1766
SsiI CCGC 2 cut(s) 209, 1655
SspI AATATT 4 cut(s) 427, 741, 1142, 1327
SspMI CTAG 3 cut(s) 1226, 1478, 1724
StyD4I CCNGG 2 cut(s) 231, 1567
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 6 cut(s) 246, 646, 1299, 1385, 1402, 1634
TaiI ACGT 2 cut(s) 60, 992
TaqI TCGA 3 cut(s) 475, 1513, 1527
TaqII GACCGA 1 cut(s) 179
TasI AATT 9 cut(s) 281, 471, 515, 813, 864, 924, 1496, 1739, 1766
TatI WGTACW 1 cut(s) 1380
TfiI GAWTC 2 cut(s) 550, 802
Tru1I TTAA 5 cut(s) 423, 468, 737, 1458, 1482
Tru9I TTAA 5 cut(s) 423, 468, 737, 1458, 1482
TscAI CASTG 6 cut(s) 133, 276, 601, 651, 1390, 1639
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 3 cut(s) 15, 64, 149
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 8 cut(s) 63, 407, 528, 708, 744, 1208, 1337, 1448
TspGWI ACGGA 2 cut(s) 38, 1096
TspRI CASTG 6 cut(s) 133, 276, 601, 651, 1390, 1639
VneI GTGCAC 1 cut(s) 746
VpaK11BI GGWCC 3 cut(s) 191, 445, 1316
XapI RAATTY 3 cut(s) 471, 813, 1739
XceI RCATGY 2 cut(s) 833, 1679
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 3 cut(s) 1226, 1478, 1724
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.