Rh5CG400200

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001514
Physical Location & Seq
Forward (+)
53301784 .. 53307104
5321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG400200.1

Sequence Viewer

Length: 1524 bp
ATGGAGACTCTATGCTTTACTCCTACATCTTCACCACAACATCTCAAAAGCCACCACACTTCATCATCAACCTCAGTCCCATCCCTCATCAGACCACTAGTTTTCAAAGACTATAACAATCCAAAGAAGAATATTAACCTCAGAAACCAACAATATTGCAAAGCTATATTAACAAACCCAGCTGAAGTGACCTCCTTCAAACCTTCCCCGCTGCCCAGTCCCTTCGAAACGACGCCGCTGCACGAACCCTCACAAGATAACTCCCATGAGTTAAGGAAGGTCCTCCCGGAGACATTGGAGTACGAGAGCGGCTTTCTCGGTTCCGTGCCGAAAAAGTTCGCCGGAGATGATGGTAACGGAATTCCCCAGACCATGATGAGCTACTTGACGAGGATTTTGACGTCTAAGGTTTATGATGTGGCCATTGAATCACCACTGGACTACGCTTCAAAGCTCTCCAACAAGCTCGGCGTTCATCTTTGGCTCAAGAGAGAGGATCTACAACCCGTTTTCTCCTTTAAACTGCGAGGAGCTTATAATATGATGGCAAAGCTTTCAAGTGAACAACTGGACAGAGGTGTCATTTGCTCATCAGCAGGGAATCATGCTCAAGGAGTTGCACTATCTGCACGAAAACTAGGGTGCACTGCCGTCATTGTCATGCCTATTACAACTCCGGAAATCAAATGGAAAACAGTGGAGAGTTTAGGTGCAACAGTTGTGCTTGTGGGAGAGTCCTATGATGAAGCTCAATCTTACGCACTACAAAGGGCCAAAGATGAAGGCCTCATATTTGTACATCCTTTTGATCACCCAGATGTGATAGCAGGTCAGGGAACTGTAGGAATGGAGATACTACGCCAAATTCAAGGCCCATTGCACGCAATCTTTGTGCCTGTTGGTGGTGGTGGACTAATTGCCGGCATTGCTTCCTATGTCAAGAGTATTCGGCCAGAGGTTAAGATCATTGGTGTAGAGTTATCCGATGCAAATGCAATGGCACTGTCATTACATCACGGTGAGAGAGTTGTGCTTGAGCGAGTTGGAGGATTTGCAGATGGCATGGCAGTCAAAAAAGTTGGGGAAGAAACTTTTCGATTGTGCCGGGAACTAATGGATGGAGTTGTTCTTGTTAATCGTGATGCCATTTGTGCATCCATAAAAGACATGTTTGAGCAAAAACGGAGCATACTTGAACCGGCAGGGGCAGCAGCCATTGCTGGAGCTGAAGCATATTGCAAATATTATGGACTGAAGGGTGAAAATGTTGTGGCAATAACCAGTGGAGCAAATATGAATTTTGATAGACTGAGGTTGGTATCGCAACTAGCTGATGTTGGTCGGAGACGTGAAGGTTTCCTTGCAACATTTGTTCCAGAAAAGTGTGGAAGCTTCAAACTTGTTTGTGAATTGGTAAGTCAAAAACATTGCTATATGTTTTATAACACTTCTTGTTCCATCTATTTTAAGCTTGTTGTCCACACTTGCCTAACAGGTGGGTCCAATGAATATCATCGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

507

Amino Acids

55.55

Weight (kDa)

7.91

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 143 - 429 4.6e-81 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 537, 1443
AasI GACNNNNNNGTC 1 cut(s) 578
AatII GACGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 818
AccBSI CCGCTC 1 cut(s) 309
AccIII TCCGGA 1 cut(s) 676
AciI CCGC 3 cut(s) 209, 236, 309
AclWI GGATC 1 cut(s) 504
AcoI YGGCCR 2 cut(s) 420, 950
AcsI RAATTY 3 cut(s) 360, 864, 1297
AcuI CTGAAG 3 cut(s) 204, 1248, 1274
AcyI GRCGYC 2 cut(s) 233, 401
AfaI GTAC 2 cut(s) 302, 798
AfiI CCNNNNNNNGG 1 cut(s) 902
AflIII ACRYGT 1 cut(s) 1167
AgsI TTSAA 8 cut(s) 106, 199, 428, 450, 558, 869, 1196, 1396
AhlI ACTAGT 1 cut(s) 97
AjiI CACGTC 1 cut(s) 1349
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 2 cut(s) 284, 1339
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 1 cut(s) 504
Aor13HI TCCGGA 1 cut(s) 676
AoxI GGCC 5 cut(s) 420, 771, 784, 871, 950
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 4 cut(s) 211, 238, 1208, 1211
ApoI RAATTY 3 cut(s) 360, 864, 1297
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
Asp700I GAANNNNTTC 2 cut(s) 335, 1092
AspS9I GGNCC 4 cut(s) 280, 771, 872, 1500
AsuC2I CCSGG 2 cut(s) 287, 1106
AsuHPI GGTGA 5 cut(s) 24, 423, 803, 1031, 1271
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 2 cut(s) 280, 1500
BaeGI GKGCMC 1 cut(s) 647
BalI TGGCCA 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 647
BbvI GCAGC 4 cut(s) 198, 225, 1220, 1223
BccI CCATC 6 cut(s) 88, 344, 538, 1052, 1112, 1466
BceAI ACGGC 1 cut(s) 635
BcgI CGANNNNNNTGC 4 cut(s) 220, 254, 974, 1008
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 2 cut(s) 287, 1106
BcoDI GTCTC 2 cut(s) 284, 1339
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 3 cut(s) 98, 638, 1328
BfmI CTRYAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 818
BisI GCNGC 6 cut(s) 212, 236, 239, 310, 1209, 1212
BlsI GCNGC 6 cut(s) 213, 237, 240, 311, 1210, 1213
Bme1390I CCNGG 2 cut(s) 287, 1106
Bme18I GGWCC 2 cut(s) 280, 1500
BmgBI CACGTC 1 cut(s) 1349
BmgT120I GGNCC 4 cut(s) 280, 771, 872, 1500
BmiI GGNNCC 2 cut(s) 322, 1501
BmrFI CCNGG 2 cut(s) 287, 1106
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 3 cut(s) 976, 1132, 1163
BmuI ACTGGG 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 1242
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 3 cut(s) 470, 594, 1055
BpuMI CCSGG 2 cut(s) 287, 1106
BsaHI GRCGYC 2 cut(s) 233, 401
BsaWI WCCGGW 1 cut(s) 676
Bsc4I CCNNNNNNNGG 1 cut(s) 902
Bse118I RCCGGY 2 cut(s) 920, 1198
Bse1I ACTGG 4 cut(s) 216, 441, 573, 1281
Bse3DI GCAATG 5 cut(s) 875, 924, 1002, 1215, 1426
BseAI TCCGGA 1 cut(s) 676
BseGI GGATG 4 cut(s) 80, 799, 1123, 1154
BseLI CCNNNNNNNGG 1 cut(s) 902
BseMI GCAATG 5 cut(s) 875, 924, 1002, 1215, 1426
BseMII CTCAG 3 cut(s) 87, 154, 1301
BseNI ACTGG 4 cut(s) 216, 441, 573, 1281
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 4 cut(s) 198, 225, 1220, 1223
BseYI CCCAGC 1 cut(s) 178
BsgI GTGCAG 2 cut(s) 224, 612
BshFI GGCC 5 cut(s) 422, 773, 786, 873, 952
BsiHKAI GWGCWC 1 cut(s) 647
BsiSI CCGG 6 cut(s) 287, 342, 677, 921, 1105, 1199
BslFI GGGAC 2 cut(s) 62, 204
BslI CCNNNNNNNGG 1 cut(s) 902
BsmAI GTCTC 2 cut(s) 284, 1339
BsmBI CGTCTC 1 cut(s) 1339
BsmFI GGGAC 2 cut(s) 62, 204
BsnI GGCC 5 cut(s) 422, 773, 786, 873, 952
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 647
Bsp13I TCCGGA 1 cut(s) 676
Bsp1407I TGTACA 1 cut(s) 796
Bsp143I GATC 3 cut(s) 496, 808, 963
BspACI CCGC 3 cut(s) 209, 236, 309
BspANI GGCC 5 cut(s) 422, 773, 786, 873, 952
BspCNI CTCAG 3 cut(s) 86, 153, 1302
BspEI TCCGGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 322, 1501
BspMI ACCTGC 1 cut(s) 818
BspPI GGATC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 309
BsrDI GCAATG 5 cut(s) 875, 924, 1002, 1215, 1426
BsrFI RCCGGY 2 cut(s) 920, 1198
BsrGI TGTACA 1 cut(s) 796
BsrI ACTGG 4 cut(s) 216, 441, 573, 1281
BssAI RCCGGY 2 cut(s) 920, 1198
BssMI GATC 3 cut(s) 496, 808, 963
BssNI GRCGYC 2 cut(s) 233, 401
Bst4CI ACNGT 5 cut(s) 697, 718, 841, 1005, 1019
BstACI GRCGYC 2 cut(s) 233, 401
BstAPI GCANNNNNTGC 2 cut(s) 626, 1217
BstAUI TGTACA 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 2 cut(s) 882, 922
BstDEI CTNAG 4 cut(s) 73, 140, 405, 1310
BstF5I GGATG 4 cut(s) 80, 799, 1123, 1154
BstKTI GATC 3 cut(s) 499, 811, 966
BstMAI GTCTC 2 cut(s) 284, 1339
BstMBI GATC 3 cut(s) 496, 808, 963
BstMWI GCNNNNNNNGC 5 cut(s) 626, 926, 1151, 1208, 1217
BstNSI RCATGY 1 cut(s) 1171
BstSCI CCNGG 2 cut(s) 285, 1104
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 647
BstV1I GCAGC 4 cut(s) 198, 225, 1220, 1223
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 5 cut(s) 422, 773, 786, 873, 952
BtrI CACGTC 1 cut(s) 1349
BtsCI GGATG 4 cut(s) 80, 799, 1123, 1154
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 5 cut(s) 434, 645, 702, 1001, 1288
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 2 cut(s) 882, 922
Cfr10I RCCGGY 2 cut(s) 920, 1198
Cfr13I GGNCC 4 cut(s) 280, 771, 872, 1500
CseI GACGC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 301, 797
CviAII CATG 6 cut(s) 266, 373, 605, 661, 1063, 1168
CviQI GTAC 2 cut(s) 301, 797
DdeI CTNAG 4 cut(s) 73, 140, 405, 1310
DpnI GATC 3 cut(s) 498, 810, 965
DpnII GATC 3 cut(s) 496, 808, 963
DraI TTTAAA 1 cut(s) 520
DrdI GACNNNNNNGTC 1 cut(s) 578
DseDI GACNNNNNNGTC 1 cut(s) 578
EaeI YGGCCR 2 cut(s) 420, 950
Eco147I AGGCCT 1 cut(s) 786
Eco47I GGWCC 2 cut(s) 280, 1500
Eco57I CTGAAG 3 cut(s) 204, 1248, 1274
EcoO109I RGGNCCY 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 1339
FaeI CATG 6 cut(s) 269, 376, 608, 664, 1066, 1171
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 2 cut(s) 62, 204
FatI CATG 6 cut(s) 265, 372, 604, 660, 1062, 1167
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 6 cut(s) 212, 236, 239, 310, 1209, 1212
FokI GGATG 4 cut(s) 67, 786, 1130, 1141
Fsp4HI GCNGC 6 cut(s) 212, 236, 239, 310, 1209, 1212
FspBI CTAG 3 cut(s) 98, 638, 1328
GluI GCNGC 6 cut(s) 212, 236, 239, 310, 1209, 1212
GsaI CCCAGC 1 cut(s) 182
GsuI CTGGAG 1 cut(s) 1242
HaeIII GGCC 5 cut(s) 422, 773, 786, 873, 952
HapII CCGG 6 cut(s) 287, 342, 677, 921, 1105, 1199
HgaI GACGC 1 cut(s) 241
Hin1I GRCGYC 2 cut(s) 233, 401
Hin1II CATG 6 cut(s) 269, 376, 608, 664, 1066, 1171
HindIII AAGCTT 3 cut(s) 551, 1390, 1469
HinfI GANTC 4 cut(s) 7, 428, 601, 734
HpaII CCGG 6 cut(s) 287, 342, 677, 921, 1105, 1199
HphI GGTGA 5 cut(s) 24, 423, 803, 1031, 1271
Hpy166II GTNNAC 4 cut(s) 563, 645, 911, 1480
Hpy188I TCNGA 4 cut(s) 92, 143, 985, 1344
Hpy188III TCNNGA 6 cut(s) 487, 677, 940, 1139, 1376, 1517
Hpy8I GTNNAC 4 cut(s) 563, 645, 911, 1480
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 7 cut(s) 205, 213, 232, 271, 776, 1249, 1346
HpyCH4III ACNGT 5 cut(s) 697, 718, 841, 1005, 1019
HpyCH4IV ACGT 2 cut(s) 401, 1348
HpyF10VI GCNNNNNNNGC 5 cut(s) 626, 926, 1151, 1208, 1217
HpyF3I CTNAG 4 cut(s) 73, 140, 405, 1310
HpySE526I ACGT 2 cut(s) 401, 1348
Hsp92I GRCGYC 2 cut(s) 233, 401
Hsp92II CATG 6 cut(s) 269, 376, 608, 664, 1066, 1171
Kpn2I TCCGGA 1 cut(s) 676
KroI GCCGGC 1 cut(s) 920
KroNI GCCGGC 1 cut(s) 922
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 3 cut(s) 496, 808, 963
LmnI GCTCC 4 cut(s) 530, 1185, 1223, 1286
Lsp1109I GCAGC 4 cut(s) 198, 225, 1220, 1223
LweI GCATC 3 cut(s) 976, 1132, 1163
MaeI CTAG 3 cut(s) 98, 638, 1328
MaeII ACGT 2 cut(s) 401, 1348
MaeIII GTNAC 2 cut(s) 187, 353
MalI GATC 3 cut(s) 498, 810, 965
MbiI CCGCTC 1 cut(s) 309
MboI GATC 3 cut(s) 496, 808, 963
MboII GAAGA 3 cut(s) 21, 139, 1097
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 1 cut(s) 647
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 5 cut(s) 360, 864, 915, 1297, 1409
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 1 cut(s) 743
MmeI TCCRAC 3 cut(s) 483, 1024, 1322
Mox20I TGGCCA 1 cut(s) 422
MroI TCCGGA 1 cut(s) 676
MroNI GCCGGC 1 cut(s) 920
MroXI GAANNNNTTC 2 cut(s) 335, 1092
MscI TGGCCA 1 cut(s) 422
MseI TTAA 7 cut(s) 135, 170, 272, 519, 960, 1134, 1467
MslI CAYNNNNRTG 3 cut(s) 659, 816, 1017
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 3 cut(s) 182, 211, 238
MspI CCGG 6 cut(s) 287, 342, 677, 921, 1105, 1199
MspR9I CCNGG 2 cut(s) 287, 1106
MwoI GCNNNNNNNGC 5 cut(s) 626, 926, 1151, 1208, 1217
NaeI GCCGGC 1 cut(s) 922
NciI CCSGG 2 cut(s) 287, 1106
NdeII GATC 3 cut(s) 496, 808, 963
NgoMIV GCCGGC 1 cut(s) 920
NlaIII CATG 6 cut(s) 269, 376, 608, 664, 1066, 1171
NlaIV GGNNCC 2 cut(s) 322, 1501
NmeAIII GCCGAG 1 cut(s) 447
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 225
PceI AGGCCT 1 cut(s) 786
PciI ACATGT 1 cut(s) 1167
PdiI GCCGGC 1 cut(s) 922
PdmI GAANNNNTTC 2 cut(s) 335, 1092
PfeI GAWTC 2 cut(s) 428, 601
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 6 cut(s) 213, 237, 240, 311, 1210, 1213
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 1167
PsiI TTATAA 2 cut(s) 537, 1443
Psp5II RGGWCCY 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 322, 1501
PspPI GGNCC 4 cut(s) 280, 771, 872, 1500
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 2 cut(s) 302, 798
RsaNI GTAC 2 cut(s) 301, 797
RseI CAYNNNNRTG 3 cut(s) 659, 816, 1017
SaqAI TTAA 7 cut(s) 135, 170, 272, 519, 960, 1134, 1467
SatI GCNGC 6 cut(s) 212, 236, 239, 310, 1209, 1212
Sau3AI GATC 3 cut(s) 496, 808, 963
Sau96I GGNCC 4 cut(s) 280, 771, 872, 1500
SchI GAGTC 1 cut(s) 743
ScrFI CCNGG 2 cut(s) 287, 1106
SduI GDGCHC 1 cut(s) 647
SfaNI GCATC 3 cut(s) 976, 1132, 1163
SfcI CTRYAG 1 cut(s) 840
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 2 cut(s) 280, 1500
SmiMI CAYNNNNRTG 3 cut(s) 659, 816, 1017
SmlI CTYRAG 3 cut(s) 485, 609, 1034
SmoI CTYRAG 3 cut(s) 485, 609, 1034
SpeI ACTAGT 1 cut(s) 97
Sse9I AATT 5 cut(s) 360, 864, 915, 1297, 1409
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 3 cut(s) 209, 236, 309
SspI AATATT 3 cut(s) 133, 155, 1244
SspMI CTAG 3 cut(s) 98, 638, 1328
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 2 cut(s) 285, 1104
TaaI ACNGT 5 cut(s) 697, 718, 841, 1005, 1019
TaiI ACGT 2 cut(s) 404, 1351
TaqI TCGA 3 cut(s) 225, 1096, 1516
TasI AATT 5 cut(s) 360, 864, 915, 1297, 1409
TatI WGTACW 1 cut(s) 796
TauI GCSGC 2 cut(s) 238, 312
TfiI GAWTC 2 cut(s) 428, 601
Tru1I TTAA 7 cut(s) 135, 170, 272, 519, 960, 1134, 1467
Tru9I TTAA 7 cut(s) 135, 170, 272, 519, 960, 1134, 1467
TscAI CASTG 5 cut(s) 441, 652, 702, 1008, 1288
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 4 cut(s) 211, 238, 1208, 1211
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 6 cut(s) 51, 464, 759, 795, 1310, 1521
TspGWI ACGGA 3 cut(s) 313, 372, 1198
TspRI CASTG 5 cut(s) 441, 652, 702, 1008, 1288
VneI GTGCAC 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 280, 1500
XapI RAATTY 3 cut(s) 360, 864, 1297
XceI RCATGY 1 cut(s) 1171
XmnI GAANNNNTTC 2 cut(s) 335, 1092
XspI CTAG 3 cut(s) 98, 638, 1328
ZraI GACGTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.