Rh5BG376900

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001514
Physical Location & Seq
Forward (+)
59705260 .. 59711399
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG376900.1

Sequence Viewer

Length: 1884 bp
ATGGAGACTCTATGCTTTACTCCTACATCTTCACCACAACATCTCAAAAACCACCACACTTCATCATCAACCTCAGTCCCATCCCTCATCAGACCACTAGTTTTCAAAGACTATAACAATCCAAAGAAGAATATTAACCTCAGAAACCAATTACAATATTGCAAAGCTATATTAACAAACCCAGCTGAAGTGACCTCCTTCAAACCTTCCCCGCTGCCCAGTCCCTTCGAAACGACGCCGCTGCACGAACCCTCACAAGATCACTCCCATGAGTTAAGGAAGGTCCTCCCGGAGACATTGGAGTACGAGAGCGGCTTTCTCGGTTCCGTGCCGAAAAAGTTCGCGGGCGATGATGGCAACGGAATTCCCCAGACCATGATGAGCTACTTGACGAGGATTTTGACGTCTAAGGTTTATGATGTGGCCATTGAATCACCACTGGACTACGCTTCAAAGCTTTCCGACAAGCTCGGCGTTCATCTTTGGCTCAAGAGAGAGGATCTACAACCCGTTTTCTCCTTTAAACTGCGAGGAGCTTATAATATGATGGCAAAGCTTGAAAGTGAACAACTGGACAGAGGTGTCATTTGCTCATCAGCAGGGAATCATGCTCAAGGAGTTGCACTATCTGCACGAAAACTAGGGTGCACTGCCGTCATTGTCATGCCTATTACAACTCCGGAAATCAAATGGAAAACAGTGGAGAGTTTAGGTGCAACAGTTGTGCTTGTGGGAGAGTCCTATGATGAAGCTCAATCTTACGCACTACAAAGGGCCAAAGATGAAGGCCTCATATTTGTACATCCTTTTGATCACCCAGATGTGATAGCAGGTCAGGGAACTGTAGGAATGGAGATACTACGCCAAATTCAAGGCCCATTGCACGCAATCTTTGTGCCTGTTGGTGGTGGTGGACTAATTGCCGGCATTGCTACCTATGTCAAGAGTATTCGGCCGGAGGTTAAGATCATTGGTGTAGAGTTATCCGATGCAAATGCAATGGCACTGTCATTACATCACGGTGAGAGAGTTGTGCTTGAGCGAGTTGGAGGATTTGCAGATGGCATGGCAGTCAAAAAAGTTGGGGAAGAAACTTTTCGATTGTGCCGGGAACTAATGGATGGAGTTGTTCTTGTTAATCGTGATGCCATTTGTGCATCCATAAAAGACATGTTTGAGCAAAAACGGAGCATACTTGAACCGGCAGGGGCAGCAGCCATTGCTGGAGCTGAAGCATATTGCAAATATTATGGACTGAAGGGTGAAAATGTTGTGGCAATAACCAGTGGAGCAAACATGAATTTTGATAGACTGAGGTTGGTATCCCAACTAGCTGATGTTGGTCGGAGACGTGAAGGTTTCCTTGCAACATTTGTTCCAGAAAAGTGTGGAAGCTTCAAACTTTTTTGTGAATTGGTGGGTCCAATGAATATCATCGAGATTAGCTACAGATTTGATGATAAAAAACAAGAAGGTCTTATCCTGTACAGTGTTTCCTTTCACAGAATTTCAGAGTTGGAAGGGATGTTGGACCGTATGAAGTTGGCTCAGCTTCAAACAATTAACCTAACAGATAAAGAACTAATCAAAGATCACATGGAATTTTTGGTGGGAGGTCGATCAAATGTCGAAAACGAGATTCTTTGCCGCCTTTTCTTTCCAGAGAGACCAGGCGCTCTGATGAAGTTCTTAAATGCTTTCAGTCCCCTTTGGAACATTACCTTGTTCCATTACAGTGGACAGGGTGAAACTGTTGCAAATGCTTTGGTGGGTTTCCAAGTCGCAAGCTCTGAGATTGACAAGTTCAAGGGTTTGGCCGATACTTTAGGATATGATTATGCATTTGAGACGAACAATGAAACCTTACAACTGCTATTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

627

Amino Acids

69.28

Weight (kDa)

6.13

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 144 - 430 1.1e-81 Pyridoxal-phosphate dependent enzyme
Thr_dehydrat_C PF00585 445 - 532 3e-14 C-terminal regulatory domain of Threonine dehydratase
Thr_dehydrat_C PF00585 538 - 626 4.8e-20 C-terminal regulatory domain of Threonine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AasI GACNNNNNNGTC 1 cut(s) 581
AatII GACGTC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 312
AccII CGCG 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 679
AciI CCGC 5 cut(s) 212, 239, 312, 344, 1648
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 3 cut(s) 423, 953, 1815
AcsI RAATTY 5 cut(s) 363, 867, 1300, 1506, 1601
AcuI CTGAAG 3 cut(s) 207, 1251, 1277
AcyI GRCGYC 2 cut(s) 236, 404
AfaI GTAC 3 cut(s) 305, 801, 1487
AfiI CCNNNNNNNGG 1 cut(s) 905
AflIII ACRYGT 1 cut(s) 1170
AhlI ACTAGT 1 cut(s) 97
AjiI CACGTC 1 cut(s) 1352
AjnI CCWGG 1 cut(s) 1669
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 4 cut(s) 287, 1342, 1660, 1841
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 1 cut(s) 507
Aor13HI TCCGGA 1 cut(s) 679
AoxI GGCC 6 cut(s) 423, 774, 787, 874, 953, 1815
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 4 cut(s) 214, 241, 1211, 1214
ApoI RAATTY 5 cut(s) 363, 867, 1300, 1506, 1601
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
Asp700I GAANNNNTTC 2 cut(s) 338, 1095
AspLEI GCGC 1 cut(s) 1676
AspS9I GGNCC 5 cut(s) 283, 774, 875, 1421, 1531
AsuC2I CCSGG 2 cut(s) 290, 1109
AsuHPI GGTGA 6 cut(s) 24, 426, 806, 1034, 1274, 1757
AsuII TTCGAA 1 cut(s) 228
AvaII GGWCC 3 cut(s) 283, 1421, 1531
BaeGI GKGCMC 1 cut(s) 650
BalI TGGCCA 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 4 cut(s) 201, 228, 1223, 1226
BccI CCATC 5 cut(s) 88, 347, 541, 1055, 1115
BceAI ACGGC 1 cut(s) 638
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 977, 1011
BciT130I CCWGG 1 cut(s) 1671
BciVI GTATCC 1 cut(s) 1333
BclI TGATCA 1 cut(s) 811
BcnI CCSGG 2 cut(s) 290, 1109
BcoDI GTCTC 4 cut(s) 287, 1342, 1660, 1841
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 3 cut(s) 98, 641, 1331
BfmI CTRYAG 2 cut(s) 843, 1447
BfoI RGCGCY 1 cut(s) 1677
BfuAI ACCTGC 1 cut(s) 821
BfuI GTATCC 1 cut(s) 1333
BisI GCNGC 7 cut(s) 215, 239, 242, 313, 1212, 1215, 1648
BlpI GCTNAGC 1 cut(s) 1548
BlsI GCNGC 7 cut(s) 216, 240, 243, 314, 1213, 1216, 1649
Bme1390I CCNGG 3 cut(s) 290, 1109, 1671
Bme18I GGWCC 3 cut(s) 283, 1421, 1531
BmgBI CACGTC 1 cut(s) 1352
BmgT120I GGNCC 5 cut(s) 283, 774, 875, 1421, 1531
BmiI GGNNCC 2 cut(s) 325, 1422
BmrFI CCNGG 3 cut(s) 290, 1109, 1671
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 979, 1135, 1166
BmuI ACTGGG 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 1245
Bpu1102I GCTNAGC 1 cut(s) 1548
Bpu14I TTCGAA 1 cut(s) 228
BpuEI CTTGAG 3 cut(s) 473, 597, 1058
BpuMI CCSGG 2 cut(s) 290, 1109
BsaHI GRCGYC 2 cut(s) 236, 404
BsaI GGTCTC 1 cut(s) 1660
BsaWI WCCGGW 1 cut(s) 679
Bsc4I CCNNNNNNNGG 1 cut(s) 905
Bse118I RCCGGY 2 cut(s) 923, 1201
Bse1I ACTGG 4 cut(s) 219, 444, 576, 1284
Bse3DI GCAATG 4 cut(s) 878, 927, 1005, 1218
BseAI TCCGGA 1 cut(s) 679
BseBI CCWGG 1 cut(s) 1671
BseGI GGATG 5 cut(s) 80, 802, 1126, 1157, 1530
BseLI CCNNNNNNNGG 1 cut(s) 905
BseMI GCAATG 4 cut(s) 878, 927, 1005, 1218
BseMII CTCAG 5 cut(s) 87, 154, 1304, 1562, 1782
BseNI ACTGG 4 cut(s) 219, 444, 576, 1284
BseRI GAGGAG 1 cut(s) 546
BseSI GKGCMC 1 cut(s) 650
BseX3I CGGCCG 1 cut(s) 953
BseXI GCAGC 4 cut(s) 201, 228, 1223, 1226
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 2 cut(s) 227, 615
Bsh1236I CGCG 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 956
BshFI GGCC 6 cut(s) 425, 776, 789, 876, 955, 1817
BsiEI CGRYCG 1 cut(s) 956
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 6 cut(s) 290, 680, 924, 956, 1108, 1202
BslFI GGGAC 3 cut(s) 62, 207, 1689
BslI CCNNNNNNNGG 1 cut(s) 905
BsmAI GTCTC 4 cut(s) 287, 1342, 1660, 1841
BsmBI CGTCTC 2 cut(s) 1342, 1841
BsmFI GGGAC 3 cut(s) 62, 207, 1689
BsnI GGCC 6 cut(s) 425, 776, 789, 876, 955, 1817
Bso31I GGTCTC 1 cut(s) 1660
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 650
Bsp13I TCCGGA 1 cut(s) 679
Bsp1407I TGTACA 2 cut(s) 799, 1485
Bsp143I GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
Bsp1720I GCTNAGC 1 cut(s) 1548
BspACI CCGC 5 cut(s) 212, 239, 312, 344, 1648
BspANI GGCC 6 cut(s) 425, 776, 789, 876, 955, 1817
BspCNI CTCAG 5 cut(s) 86, 153, 1305, 1561, 1783
BspEI TCCGGA 1 cut(s) 679
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 2 cut(s) 325, 1422
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 1 cut(s) 507
BspT104I TTCGAA 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 1660
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 4 cut(s) 878, 927, 1005, 1218
BsrFI RCCGGY 2 cut(s) 923, 1201
BsrGI TGTACA 2 cut(s) 799, 1485
BsrI ACTGG 4 cut(s) 219, 444, 576, 1284
BssAI RCCGGY 2 cut(s) 923, 1201
BssMI GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
BssNI GRCGYC 2 cut(s) 236, 404
Bst2UI CCWGG 1 cut(s) 1671
Bst4CI ACNGT 9 cut(s) 700, 721, 844, 1008, 1022, 1490, 1535, 1736, 1753
BstACI GRCGYC 2 cut(s) 236, 404
BstAPI GCANNNNNTGC 2 cut(s) 629, 1220
BstAUI TGTACA 2 cut(s) 799, 1485
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 4 cut(s) 346, 885, 925, 1786
BstDEI CTNAG 6 cut(s) 73, 140, 408, 1313, 1548, 1791
BstF5I GGATG 5 cut(s) 80, 802, 1126, 1157, 1530
BstFNI CGCG 1 cut(s) 344
BstH2I RGCGCY 1 cut(s) 1677
BstHHI GCGC 1 cut(s) 1676
BstKTI GATC 6 cut(s) 262, 502, 814, 969, 1594, 1622
BstMAI GTCTC 4 cut(s) 287, 1342, 1660, 1841
BstMBI GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
BstMCI CGRYCG 1 cut(s) 956
BstMWI GCNNNNNNNGC 6 cut(s) 354, 629, 929, 1154, 1211, 1220
BstNI CCWGG 1 cut(s) 1671
BstNSI RCATGY 1 cut(s) 1174
BstSCI CCNGG 3 cut(s) 288, 1107, 1669
BstSFI CTRYAG 2 cut(s) 843, 1447
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 4 cut(s) 201, 228, 1223, 1226
BstX2I RGATCY 1 cut(s) 499
BstXI CCANNNNNNTGG 1 cut(s) 1736
BstYI RGATCY 1 cut(s) 499
BstZI CGGCCG 1 cut(s) 953
BsuI GTATCC 1 cut(s) 1333
BsuRI GGCC 6 cut(s) 425, 776, 789, 876, 955, 1817
BtgZI GCGATG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 1352
BtsCI GGATG 5 cut(s) 80, 802, 1126, 1157, 1530
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 7 cut(s) 437, 648, 705, 1004, 1291, 1495, 1741
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 4 cut(s) 346, 885, 925, 1786
CfoI GCGC 1 cut(s) 1676
Cfr10I RCCGGY 2 cut(s) 923, 1201
Cfr13I GGNCC 5 cut(s) 283, 774, 875, 1421, 1531
CseI GACGC 1 cut(s) 244
Csp6I GTAC 3 cut(s) 304, 800, 1486
CviAII CATG 8 cut(s) 269, 376, 608, 664, 1066, 1171, 1297, 1597
CviQI GTAC 3 cut(s) 304, 800, 1486
DdeI CTNAG 6 cut(s) 73, 140, 408, 1313, 1548, 1791
DpnI GATC 6 cut(s) 261, 501, 813, 968, 1593, 1621
DpnII GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
DraI TTTAAA 1 cut(s) 523
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 3 cut(s) 423, 953, 1815
EagI CGGCCG 1 cut(s) 953
EclXI CGGCCG 1 cut(s) 953
Eco147I AGGCCT 1 cut(s) 789
Eco31I GGTCTC 1 cut(s) 1660
Eco47I GGWCC 3 cut(s) 283, 1421, 1531
Eco52I CGGCCG 1 cut(s) 953
Eco57I CTGAAG 3 cut(s) 207, 1251, 1277
EcoO109I RGGNCCY 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 1669
EcoT22I ATGCAT 1 cut(s) 1843
Esp3I CGTCTC 2 cut(s) 1342, 1841
FaeI CATG 8 cut(s) 272, 379, 611, 667, 1069, 1174, 1300, 1600
FalI AAGNNNNNCTT 4 cut(s) 1347, 1379, 1461, 1493
FaqI GGGAC 3 cut(s) 62, 207, 1689
FatI CATG 8 cut(s) 268, 375, 607, 663, 1065, 1170, 1296, 1596
FauI CCCGC 2 cut(s) 219, 337
FbaI TGATCA 1 cut(s) 811
Fnu4HI GCNGC 7 cut(s) 215, 239, 242, 313, 1212, 1215, 1648
FokI GGATG 5 cut(s) 67, 789, 1133, 1144, 1537
Fsp4HI GCNGC 7 cut(s) 215, 239, 242, 313, 1212, 1215, 1648
FspBI CTAG 3 cut(s) 98, 641, 1331
GlaI GCGC 1 cut(s) 1675
GluI GCNGC 7 cut(s) 215, 239, 242, 313, 1212, 1215, 1648
GsaI CCCAGC 1 cut(s) 185
GsuI CTGGAG 1 cut(s) 1245
HaeII RGCGCY 1 cut(s) 1677
HaeIII GGCC 6 cut(s) 425, 776, 789, 876, 955, 1817
HapII CCGG 6 cut(s) 290, 680, 924, 956, 1108, 1202
HgaI GACGC 1 cut(s) 244
HhaI GCGC 1 cut(s) 1676
Hin1I GRCGYC 2 cut(s) 236, 404
Hin1II CATG 8 cut(s) 272, 379, 611, 667, 1069, 1174, 1300, 1600
Hin6I GCGC 1 cut(s) 1674
HinP1I GCGC 1 cut(s) 1674
HindIII AAGCTT 3 cut(s) 455, 554, 1393
HinfI GANTC 5 cut(s) 7, 431, 604, 737, 1639
HpaII CCGG 6 cut(s) 290, 680, 924, 956, 1108, 1202
HphI GGTGA 6 cut(s) 24, 426, 806, 1034, 1274, 1757
Hpy166II GTNNAC 4 cut(s) 566, 648, 914, 1739
Hpy188I TCNGA 8 cut(s) 92, 143, 463, 988, 1347, 1513, 1680, 1792
Hpy188III TCNNGA 7 cut(s) 490, 680, 943, 1142, 1379, 1438, 1661
Hpy8I GTNNAC 4 cut(s) 566, 648, 914, 1739
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 9 cut(s) 208, 216, 235, 274, 779, 1252, 1349, 1466, 1514
HpyCH4III ACNGT 9 cut(s) 700, 721, 844, 1008, 1022, 1490, 1535, 1736, 1753
HpyCH4IV ACGT 2 cut(s) 404, 1351
HpyF10VI GCNNNNNNNGC 6 cut(s) 354, 629, 929, 1154, 1211, 1220
HpyF3I CTNAG 6 cut(s) 73, 140, 408, 1313, 1548, 1791
HpySE526I ACGT 2 cut(s) 404, 1351
Hsp92I GRCGYC 2 cut(s) 236, 404
Hsp92II CATG 8 cut(s) 272, 379, 611, 667, 1069, 1174, 1300, 1600
HspAI GCGC 1 cut(s) 1674
Kpn2I TCCGGA 1 cut(s) 679
KroI GCCGGC 1 cut(s) 923
KroNI GCCGGC 1 cut(s) 925
Ksp22I TGATCA 1 cut(s) 811
Kzo9I GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
LmnI GCTCC 4 cut(s) 533, 1188, 1226, 1289
Lsp1109I GCAGC 4 cut(s) 201, 228, 1223, 1226
LweI GCATC 3 cut(s) 979, 1135, 1166
MaeI CTAG 3 cut(s) 98, 641, 1331
MaeII ACGT 2 cut(s) 404, 1351
MaeIII GTNAC 1 cut(s) 190
MalI GATC 6 cut(s) 261, 501, 813, 968, 1593, 1621
MbiI CCGCTC 1 cut(s) 312
MboI GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
MboII GAAGA 3 cut(s) 21, 139, 1100
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 650
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 9 cut(s) 149, 363, 867, 918, 1300, 1412, 1506, 1560, 1601
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 746
MmeI TCCRAC 5 cut(s) 486, 1027, 1325, 1497, 1509
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 1843
MroI TCCGGA 1 cut(s) 679
MroNI GCCGGC 1 cut(s) 923
MroXI GAANNNNTTC 2 cut(s) 338, 1095
MscI TGGCCA 1 cut(s) 425
MseI TTAA 8 cut(s) 135, 173, 275, 522, 963, 1137, 1563, 1691
MslI CAYNNNNRTG 5 cut(s) 267, 662, 819, 1020, 1734
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 3 cut(s) 185, 214, 241
MspI CCGG 6 cut(s) 290, 680, 924, 956, 1108, 1202
MspR9I CCNGG 3 cut(s) 290, 1109, 1671
MvaI CCWGG 1 cut(s) 1671
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 6 cut(s) 354, 629, 929, 1154, 1211, 1220
NaeI GCCGGC 1 cut(s) 925
NciI CCSGG 2 cut(s) 290, 1109
NdeII GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
NgoMIV GCCGGC 1 cut(s) 923
NlaIII CATG 8 cut(s) 272, 379, 611, 667, 1069, 1174, 1300, 1600
NlaIV GGNNCC 2 cut(s) 325, 1422
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 1843
NspI RCATGY 1 cut(s) 1174
NspV TTCGAA 1 cut(s) 228
PceI AGGCCT 1 cut(s) 789
PciI ACATGT 1 cut(s) 1170
PdiI GCCGGC 1 cut(s) 925
PdmI GAANNNNTTC 2 cut(s) 338, 1095
PfeI GAWTC 3 cut(s) 431, 604, 1639
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 7 cut(s) 216, 240, 243, 314, 1213, 1216, 1649
PleI GAGTC 1 cut(s) 745
PpsI GAGTC 1 cut(s) 745
PpuMI RGGWCCY 1 cut(s) 283
PscI ACATGT 1 cut(s) 1170
PsiI TTATAA 1 cut(s) 540
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 1669
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 1 cut(s) 1669
PspN4I GGNNCC 2 cut(s) 325, 1422
PspPI GGNCC 5 cut(s) 283, 774, 875, 1421, 1531
PspPPI RGGWCCY 1 cut(s) 283
PsuI RGATCY 1 cut(s) 499
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 3 cut(s) 305, 801, 1487
RsaNI GTAC 3 cut(s) 304, 800, 1486
RseI CAYNNNNRTG 5 cut(s) 267, 662, 819, 1020, 1734
SaqAI TTAA 8 cut(s) 135, 173, 275, 522, 963, 1137, 1563, 1691
SatI GCNGC 7 cut(s) 215, 239, 242, 313, 1212, 1215, 1648
Sau3AI GATC 6 cut(s) 259, 499, 811, 966, 1591, 1619
Sau96I GGNCC 5 cut(s) 283, 774, 875, 1421, 1531
SchI GAGTC 1 cut(s) 746
ScrFI CCNGG 3 cut(s) 290, 1109, 1671
SduI GDGCHC 1 cut(s) 650
SfaNI GCATC 3 cut(s) 979, 1135, 1166
SfcI CTRYAG 2 cut(s) 843, 1447
SfuI TTCGAA 1 cut(s) 228
SinI GGWCC 3 cut(s) 283, 1421, 1531
SmiMI CAYNNNNRTG 5 cut(s) 267, 662, 819, 1020, 1734
SmlI CTYRAG 3 cut(s) 488, 612, 1037
SmoI CTYRAG 3 cut(s) 488, 612, 1037
SpeI ACTAGT 1 cut(s) 97
Sse9I AATT 9 cut(s) 149, 363, 867, 918, 1300, 1412, 1506, 1560, 1601
SseBI AGGCCT 1 cut(s) 789
SsiI CCGC 5 cut(s) 212, 239, 312, 344, 1648
SspI AATATT 3 cut(s) 133, 158, 1247
SspMI CTAG 3 cut(s) 98, 641, 1331
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 3 cut(s) 288, 1107, 1669
TaaI ACNGT 9 cut(s) 700, 721, 844, 1008, 1022, 1490, 1535, 1736, 1753
TaiI ACGT 2 cut(s) 407, 1354
TaqI TCGA 5 cut(s) 228, 1099, 1437, 1618, 1629
TasI AATT 9 cut(s) 149, 363, 867, 918, 1300, 1412, 1506, 1560, 1601
TatI WGTACW 2 cut(s) 799, 1485
TauI GCSGC 3 cut(s) 241, 315, 1650
TfiI GAWTC 3 cut(s) 431, 604, 1639
Tru1I TTAA 8 cut(s) 135, 173, 275, 522, 963, 1137, 1563, 1691
Tru9I TTAA 8 cut(s) 135, 173, 275, 522, 963, 1137, 1563, 1691
TscAI CASTG 7 cut(s) 444, 655, 705, 1011, 1291, 1495, 1741
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 4 cut(s) 214, 241, 1211, 1214
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 9 cut(s) 51, 467, 762, 798, 1313, 1442, 1553, 1697, 1872
TspGWI ACGGA 3 cut(s) 316, 375, 1201
TspRI CASTG 7 cut(s) 444, 655, 705, 1011, 1291, 1495, 1741
VneI GTGCAC 1 cut(s) 646
VpaK11BI GGWCC 3 cut(s) 283, 1421, 1531
XapI RAATTY 5 cut(s) 363, 867, 1300, 1506, 1601
XceI RCATGY 1 cut(s) 1174
XmnI GAANNNNTTC 2 cut(s) 338, 1095
XspI CTAG 3 cut(s) 98, 641, 1331
ZraI GACGTC 1 cut(s) 405
Zsp2I ATGCAT 1 cut(s) 1843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.