pycom11g12560

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001514
Physical Location & Seq
Reverse (-)
11473032 .. 11474724
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g12560.1

Sequence Viewer

Length: 741 bp
ATGCAGGTCAAGATAATTGGGGTAGAGGTATCCGATGCAAACGCAATGGCACTATCCATACATCATGGTCAGAGAATTATGTTGGAGAAAGTGGGAGGATTTGCAGATGGAATAGCAGTAAAAAAGGTTGGGGAAGAAACTTTTCGGTTATGCAGAGAACTCATTGATGGAGTAGTTCTTGTTAGTCGTGATGCCGTTTGTGCATCAATAAAAGATATGTTTGAGGAAAAACGGAGCATACTTGAACCGGCAGGCGCTGTAGCCATCGCTGGAGCAGAAGCATATTGCAAATATTATGGACTGAAGGGTGAAAATGTTGTTGCTATAAGTAGTGGAGCTAACATGAACTTTGACAGACTAAGATTGGTATCTCAACTAGCCGATGTTGGTAGGAGATGCGAAGTTTTCCTTGCAACATTTGTTCCAGAAAAGCGTGGAAGCTTCAAACAGTTTTGTGAACTGGTGGGAGGTCGATCGTCCTCTATCGAAAATGAGATCCTTTGCCAGTTTGCCATTCCAGCGAGGCCAGGTGCTTTATTGAAGTTCTTAGATGCTTTCAGTCCCCTTTGGAACATAAGCTTGTTCCATTACAGTGGACAGGGTGAAAGTGGTGCGGATGCGTTGGTCGGTTTACATGTTGCAAGCTCTGAGATAAACAAGTTCAAGGCTTTGGCTGATGCTCTAGGGTTTAATTATAAATTTGAGACAAACAATGAGACCTTACAATTGCTATTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

247

Amino Acids

26.75

Weight (kDa)

6.0

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 3 - 112 2.4e-16 Pyridoxal-phosphate dependent enzyme
Thr_dehydrat_C PF00585 157 - 245 2.6e-18 C-terminal regulatory domain of Threonine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AciI CCGC 1 cut(s) 614
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 4 cut(s) 245, 445, 541, 664
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 4 cut(s) 338, 441, 579, 645
AluI AGCT 4 cut(s) 338, 441, 579, 645
Alw26I GTCTC 2 cut(s) 698, 710
AlwI GGATC 1 cut(s) 490
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 524
ApoI RAATTY 1 cut(s) 698
Asp700I GAANNNNTTC 1 cut(s) 141
AspLEI GCGC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 320, 614
BccI CCATC 3 cut(s) 101, 161, 272
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 698, 710
BfaI CTAG 2 cut(s) 377, 683
BfmI CTRYAG 1 cut(s) 258
BfoI RGCGCY 1 cut(s) 258
BfuI GTATCC 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 528
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 7 cut(s) 25, 181, 212, 386, 541, 607, 667
BpmI CTGGAG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 367
BsaI GGTCTC 1 cut(s) 710
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 2 cut(s) 465, 505
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 367
BseBI CCWGG 1 cut(s) 528
BseGI GGATG 1 cut(s) 622
BseJI GATNNNNATC 1 cut(s) 367
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 2 cut(s) 465, 505
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 526
BsiEI CGRYCG 1 cut(s) 476
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 546
BsmAI GTCTC 2 cut(s) 698, 710
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 526
Bso31I GGTCTC 1 cut(s) 710
Bsp143I GATC 2 cut(s) 473, 495
BspACI CCGC 1 cut(s) 614
BspANI GGCC 1 cut(s) 526
BspCNI CTCAG 1 cut(s) 640
BspPI GGATC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 2 cut(s) 465, 505
BssAI RCCGGY 1 cut(s) 247
BssMI GATC 2 cut(s) 473, 495
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 450, 593
BstC8I GCNNGC 2 cut(s) 253, 643
BstDEI CTNAG 3 cut(s) 359, 547, 648
BstF5I GGATG 1 cut(s) 622
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 2 cut(s) 476, 498
BstMAI GTCTC 2 cut(s) 698, 710
BstMBI GATC 2 cut(s) 473, 495
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 2 cut(s) 200, 518
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 638
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 593
BstYI RGATCY 1 cut(s) 495
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 526
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 622
BtsIMutI CAGTG 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 253, 643
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 247
CviAII CATG 3 cut(s) 65, 343, 635
CviJI RGCY 9 cut(s) 263, 338, 380, 441, 526, 579, 645, 668, 674
CviKI_1 RGCY 9 cut(s) 263, 338, 380, 441, 526, 579, 645, 668, 674
DdeI CTNAG 3 cut(s) 359, 547, 648
DpnI GATC 2 cut(s) 475, 497
DpnII GATC 2 cut(s) 473, 495
Eco31I GGTCTC 1 cut(s) 710
Eco57I CTGAAG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 526
FaeI CATG 3 cut(s) 68, 346, 638
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FaqI GGGAC 1 cut(s) 546
FatI CATG 3 cut(s) 64, 342, 634
FokI GGATG 1 cut(s) 629
FspBI CTAG 2 cut(s) 377, 683
GlaI GCGC 1 cut(s) 256
GsuI CTGGAG 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 1 cut(s) 526
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 257
Hin1II CATG 3 cut(s) 68, 346, 638
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HindIII AAGCTT 2 cut(s) 439, 577
HpaII CCGG 1 cut(s) 248
HphI GGTGA 2 cut(s) 320, 614
Hpy166II GTNNAC 3 cut(s) 458, 596, 632
Hpy188I TCNGA 3 cut(s) 34, 72, 649
Hpy188III TCNNGA 3 cut(s) 10, 188, 425
Hpy8I GTNNAC 3 cut(s) 458, 596, 632
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 2 cut(s) 450, 593
HpyCH4V TGCA 9 cut(s) 4, 38, 104, 153, 203, 288, 413, 641, 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 518
HpyF3I CTNAG 3 cut(s) 359, 547, 648
Hsp92II CATG 3 cut(s) 68, 346, 638
HspAI GCGC 1 cut(s) 255
Kzo9I GATC 2 cut(s) 473, 495
LmnI GCTCC 3 cut(s) 234, 272, 335
LweI GCATC 7 cut(s) 25, 181, 212, 386, 541, 607, 667
MaeI CTAG 2 cut(s) 377, 683
MalI GATC 2 cut(s) 475, 497
MboI GATC 2 cut(s) 473, 495
MboII GAAGA 1 cut(s) 146
MfeI CAATTG 1 cut(s) 725
MflI RGATCY 1 cut(s) 495
MluCI AATT 5 cut(s) 15, 75, 691, 698, 725
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 6 cut(s) 19, 89, 217, 461, 490, 516
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 528
MunI CAATTG 1 cut(s) 725
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 2 cut(s) 200, 518
NdeII GATC 2 cut(s) 473, 495
NlaIII CATG 3 cut(s) 68, 346, 638
NspI RCATGY 1 cut(s) 638
PciI ACATGT 1 cut(s) 634
PdmI GAANNNNTTC 1 cut(s) 141
Ple19I CGATCG 1 cut(s) 476
PscI ACATGT 1 cut(s) 634
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 495
PvuI CGATCG 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 1 cut(s) 690
Sau3AI GATC 2 cut(s) 473, 495
ScrFI CCNGG 1 cut(s) 528
SfaNI GCATC 7 cut(s) 25, 181, 212, 386, 541, 607, 667
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 591
Sse9I AATT 5 cut(s) 15, 75, 691, 698, 725
SsiI CCGC 1 cut(s) 614
SspI AATATT 1 cut(s) 293
SspMI CTAG 2 cut(s) 377, 683
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 2 cut(s) 450, 593
TaqI TCGA 2 cut(s) 472, 486
TasI AATT 5 cut(s) 15, 75, 691, 698, 725
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 1 cut(s) 598
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 1 cut(s) 598
XapI RAATTY 1 cut(s) 698
XceI RCATGY 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 2 cut(s) 377, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.