Prupe.6G116600_v2.0.a1

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001514
Physical Location & Seq
Reverse (-)
8482591 .. 8485555
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G116600.1

Sequence Viewer

Length: 1254 bp
ATGGAGAGGCTCTGCCTGGCTCCTTCGCCCTCATCTCTGCTTCTCACTACACGGCACAATAATCAAAAGAAGAACCTTAGACACCAACCATGCATCATCACTGCTTTGTTCACAAACCCAGCAGAAGAGATTTCCCTCAAACCTTCAACACCACTCAACTCTTTCGAAAAGGCACCGCACAAACCCTTCCACCAAACTCAGCCGTTGAAGAAAGTCTCCGCGGAGTCTTTGAAGTACGAGAGTGGCTTTCTTGGTGCGGTGCCTGATAAGTCTGGCGGAGATAATGGAAGAGAAGGAAACAATAACATTCATGATGCCATGAGATACTTGACAAATATTTTGATGTCTAAGGTTTATGATGTTTCCATTGAGTCGCCATTGGACTATGCTCCAAAGCTCTCTGAGAAGCTCCATGCTAATCTTTGGCTCAAGAGAGAGGACCTACAACCTGTCTTCTCCTTTAAAATCCGAGGAGCTTACAATATGATGGCTAATCTTTCAAAACAACAATTAGCCAGGGGTGTCATTTGTTCATCAGCGGGGAATCATGCTCAGGGAGTTGCAGTATCAGCACAAAAACTTGGTTGCAGTGCTGTGATAGTCATGCCTCTTACAACTCCTAAAATCAAACGTGAGTCGTATGACGAAGCCGAAGCGTATGCCCAACAAAGGGCCAAAGATGAAGGCTTAATATTTGTGCATCCTTTTGATCACCCGGATGTCATAGCAGGCCAAGGAACCATAGGAATGGAGATCATGCGTCAAATTCAAGGCCCATTGCATGCAATCTTTGTGCCTGTGGGTGGTGGAGGACTCATAGCTGGTATTGCTGCCTATGTGAAGAGAGTTCACCCCAAGGTGAAGATTATTGGTGTAGAGGCATATGATGCAAATGCAATGGCACTATCCATACATCATGGGAAGAGAATTATGTTAGAAAGAGCGGGAGGATTTCACAGATGGCATAGCATAAAAAAAGTCGGGGAAGAAACTTTCCGTTTATGCAGAGAACTCATCGATGGATTTGTTCTTGTCGATCATGATGCCATTTGTGCATCAATTAAAGATATGTTTGAGGAAAATCGAAGCATACTTGAACCAGCGGGAGCCGTAGCCATTGCTGGGGCAGAAACATATTGCAAATATTATGGGCTGAAGGGTGAAAATGTTGTGGCTATAACCAGTGGAGCTAACATGAACTTTGACAGACTAGGGTTGGTATCCCAACTTGCTGTTGGTAGGAGATGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

418

Amino Acids

45.76

Weight (kDa)

8.86

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 172, 259
AccBSI CCGCTC 1 cut(s) 944
AccII CGCG 1 cut(s) 221
AciI CCGC 8 cut(s) 176, 219, 221, 257, 276, 539, 944, 1103
AcsI RAATTY 1 cut(s) 765
AcuI CTGAAG 1 cut(s) 1175
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 4 cut(s) 669, 670, 803, 1122
AgsI TTSAA 6 cut(s) 147, 208, 232, 501, 770, 1097
AjnI CCWGG 2 cut(s) 15, 515
AluBI AGCT 5 cut(s) 397, 409, 476, 821, 1190
AluI AGCT 5 cut(s) 397, 409, 476, 821, 1190
Alw26I GTCTC 1 cut(s) 220
AoxI GGCC 3 cut(s) 672, 730, 772
ApeKI GCWGC 1 cut(s) 830
ApoI RAATTY 1 cut(s) 765
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
AspS9I GGNCC 3 cut(s) 439, 672, 773
AsuC2I CCSGG 1 cut(s) 716
AsuHPI GGTGA 4 cut(s) 704, 842, 871, 1172
AsuII TTCGAA 1 cut(s) 165
AvaII GGWCC 1 cut(s) 439
BanI GGYRCC 2 cut(s) 172, 259
BbsI GAAGAC 1 cut(s) 445
BbvI GCAGC 1 cut(s) 817
BccI CCATC 3 cut(s) 481, 954, 1013
BceAI ACGGC 3 cut(s) 68, 187, 1094
BcgI CGANNNNNNTGC 4 cut(s) 641, 675, 1025, 1059
BciT130I CCWGG 2 cut(s) 17, 517
BciVI GTATCC 1 cut(s) 1231
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 716
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 1211
BfuI GTATCC 1 cut(s) 1231
BisI GCNGC 1 cut(s) 831
BlsI GCNGC 1 cut(s) 832
Bme1390I CCNGG 3 cut(s) 17, 517, 716
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 439, 672, 773
BmiI GGNNCC 5 cut(s) 21, 174, 261, 739, 1108
BmrFI CCNGG 3 cut(s) 17, 517, 716
BmsI GCATC 6 cut(s) 102, 304, 709, 877, 1033, 1064
BpiI GAAGAC 1 cut(s) 445
Bpu10I CCTNAGC 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 716
Bsa29I ATCGAT 1 cut(s) 1017
BsaJI CCNNGG 5 cut(s) 219, 469, 516, 733, 855
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 4 cut(s) 669, 670, 803, 1122
Bse1I ACTGG 1 cut(s) 1182
Bse3DI GCAATG 3 cut(s) 776, 903, 1116
BseBI CCWGG 2 cut(s) 17, 517
BseCI ATCGAT 1 cut(s) 1017
BseDI CCNNGG 5 cut(s) 219, 469, 516, 733, 855
BseGI GGATG 2 cut(s) 700, 724
BseLI CCNNNNNNNGG 4 cut(s) 669, 670, 803, 1122
BseMI GCAATG 3 cut(s) 776, 903, 1116
BseMII CTCAG 3 cut(s) 212, 393, 566
BseNI ACTGG 1 cut(s) 1182
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 1 cut(s) 817
BseYI CCCAGC 2 cut(s) 118, 1121
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 3 cut(s) 674, 732, 774
BshNI GGYRCC 2 cut(s) 172, 259
BshVI ATCGAT 1 cut(s) 1017
BsiSI CCGG 1 cut(s) 716
BslI CCNNNNNNNGG 4 cut(s) 669, 670, 803, 1122
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 3 cut(s) 674, 732, 774
Bsp119I TTCGAA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 709, 753, 1036
BspACI CCGC 8 cut(s) 176, 219, 221, 257, 276, 539, 944, 1103
BspANI GGCC 3 cut(s) 674, 732, 774
BspCNI CTCAG 3 cut(s) 211, 394, 565
BspDI ATCGAT 1 cut(s) 1017
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 2 cut(s) 310, 1039
BspLI GGNNCC 5 cut(s) 21, 174, 261, 739, 1108
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 172, 259
BsrBI CCGCTC 1 cut(s) 944
BsrDI GCAATG 3 cut(s) 776, 903, 1116
BsrI ACTGG 1 cut(s) 1182
BssECI CCNNGG 5 cut(s) 219, 469, 516, 733, 855
BssMI GATC 3 cut(s) 709, 753, 1036
BssT1I CCWWGG 2 cut(s) 733, 855
Bst2UI CCWGG 2 cut(s) 17, 517
Bst6I CTCTTC 4 cut(s) 120, 283, 836, 917
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 730, 783
BstDEI CTNAG 5 cut(s) 77, 198, 348, 402, 552
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 700, 724
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 3 cut(s) 712, 756, 1039
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 3 cut(s) 709, 753, 1036
BstMWI GCNNNNNNNGC 4 cut(s) 569, 827, 887, 1052
BstNI CCWGG 2 cut(s) 17, 517
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 3 cut(s) 15, 515, 714
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 817
BstV2I GAAGAC 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 748
Bsu15I ATCGAT 1 cut(s) 1017
BsuI GTATCC 1 cut(s) 1231
BsuRI GGCC 3 cut(s) 674, 732, 774
BsuTUI ATCGAT 1 cut(s) 1017
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 700, 724
BtsI GCAGTG 2 cut(s) 99, 595
BtsIMutI CAGTG 3 cut(s) 99, 595, 1189
Cac8I GCNNGC 2 cut(s) 730, 783
CciI TCATGA 2 cut(s) 310, 1039
Cfr13I GGNCC 3 cut(s) 439, 672, 773
Cfr42I CCGCGG 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 1017
CseI GACGC 1 cut(s) 749
Csp6I GTAC 1 cut(s) 235
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 5 cut(s) 77, 198, 348, 402, 552
DpnI GATC 3 cut(s) 711, 755, 1038
DpnII GATC 3 cut(s) 709, 753, 1036
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 4 cut(s) 120, 283, 836, 917
EarI CTCTTC 4 cut(s) 120, 283, 836, 917
EciI GGCGGA 1 cut(s) 291
Eco130I CCWWGG 2 cut(s) 733, 855
Eco47I GGWCC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 1175
EcoO109I RGGNCCY 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 15, 515
EcoT14I CCWWGG 2 cut(s) 733, 855
EcoT22I ATGCAT 1 cut(s) 95
ErhI CCWWGG 2 cut(s) 733, 855
FauI CCCGC 3 cut(s) 532, 937, 1096
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 1 cut(s) 831
FokI GGATG 2 cut(s) 687, 731
Fsp4HI GCNGC 1 cut(s) 831
FspBI CTAG 1 cut(s) 1211
GluI GCNGC 1 cut(s) 831
GsaI CCCAGC 2 cut(s) 122, 1125
HaeIII GGCC 3 cut(s) 674, 732, 774
HapII CCGG 1 cut(s) 716
HgaI GACGC 1 cut(s) 749
HinfI GANTC 5 cut(s) 224, 371, 544, 635, 813
HpaII CCGG 1 cut(s) 716
HphI GGTGA 4 cut(s) 704, 842, 871, 1172
Hpy166II GTNNAC 2 cut(s) 111, 850
Hpy188I TCNGA 2 cut(s) 403, 470
Hpy188III TCNNGA 3 cut(s) 311, 430, 1040
Hpy8I GTNNAC 2 cut(s) 111, 850
HpyAV CCTTC 6 cut(s) 33, 153, 196, 287, 677, 1150
HpyCH4IV ACGT 1 cut(s) 631
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 827, 887, 1052
HpyF3I CTNAG 5 cut(s) 77, 198, 348, 402, 552
HpySE526I ACGT 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 709
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 3 cut(s) 709, 753, 1036
LmnI GCTCC 6 cut(s) 25, 394, 414, 473, 1106, 1187
Lsp1109I GCAGC 1 cut(s) 817
LweI GCATC 6 cut(s) 102, 304, 709, 877, 1033, 1064
MaeI CTAG 1 cut(s) 1211
MaeII ACGT 1 cut(s) 631
MalI GATC 3 cut(s) 711, 755, 1038
MbiI CCGCTC 1 cut(s) 944
MboI GATC 3 cut(s) 709, 753, 1036
MboII GAAGA 9 cut(s) 82, 137, 220, 300, 445, 853, 874, 934, 998
MluCI AATT 4 cut(s) 509, 765, 927, 1059
MlyI GAGTC 4 cut(s) 233, 380, 644, 807
MnlI CCTC 9 cut(s) 40, 146, 430, 464, 618, 803, 871, 941, 1069
Mph1103I ATGCAT 1 cut(s) 95
MseI TTAA 3 cut(s) 462, 689, 1062
MslI CAYNNNNRTG 2 cut(s) 717, 746
MspA1I CMGCKG 3 cut(s) 221, 539, 1103
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 3 cut(s) 17, 517, 716
MvaI CCWGG 2 cut(s) 17, 517
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 569, 827, 887, 1052
NciI CCSGG 1 cut(s) 716
NdeI CATATG 1 cut(s) 883
NdeII GATC 3 cut(s) 709, 753, 1036
NlaIV GGNNCC 5 cut(s) 21, 174, 261, 739, 1108
NsiI ATGCAT 1 cut(s) 95
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 165
PaeI GCATGC 1 cut(s) 785
PagI TCATGA 2 cut(s) 310, 1039
PfeI GAWTC 1 cut(s) 544
PkrI GCNGC 1 cut(s) 832
PleI GAGTC 4 cut(s) 232, 379, 643, 807
PpsI GAGTC 4 cut(s) 232, 379, 643, 807
PpuMI RGGWCCY 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 2 cut(s) 15, 515
PspFI CCCAGC 2 cut(s) 118, 1121
PspGI CCWGG 2 cut(s) 15, 515
PspN4I GGNNCC 5 cut(s) 21, 174, 261, 739, 1108
PspPI GGNCC 3 cut(s) 439, 672, 773
PspPPI RGGWCCY 1 cut(s) 439
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 2 cut(s) 717, 746
SacII CCGCGG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 462, 689, 1062
SatI GCNGC 1 cut(s) 831
Sau3AI GATC 3 cut(s) 709, 753, 1036
Sau96I GGNCC 3 cut(s) 439, 672, 773
SchI GAGTC 4 cut(s) 233, 380, 644, 807
ScrFI CCNGG 3 cut(s) 17, 517, 716
SfaNI GCATC 6 cut(s) 102, 304, 709, 877, 1033, 1064
Sfr303I CCGCGG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 2 cut(s) 717, 746
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SphI GCATGC 1 cut(s) 785
Sse9I AATT 4 cut(s) 509, 765, 927, 1059
SsiI CCGC 8 cut(s) 176, 219, 221, 257, 276, 539, 944, 1103
SspI AATATT 3 cut(s) 337, 693, 1145
SspMI CTAG 1 cut(s) 1211
StyD4I CCNGG 3 cut(s) 15, 515, 714
StyI CCWWGG 2 cut(s) 733, 855
TaiI ACGT 1 cut(s) 634
TaqI TCGA 4 cut(s) 165, 1017, 1035, 1084
TasI AATT 4 cut(s) 509, 765, 927, 1059
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 3 cut(s) 462, 689, 1062
Tru9I TTAA 3 cut(s) 462, 689, 1062
TscAI CASTG 3 cut(s) 106, 595, 1189
TseI GCWGC 1 cut(s) 830
TspDTI ATGAA 4 cut(s) 299, 522, 696, 1211
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 3 cut(s) 106, 595, 1189
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 1 cut(s) 765
XceI RCATGY 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 1232
XspI CTAG 1 cut(s) 1211
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.