Rroxscaffold_1G00024460

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001514
Physical Location & Seq
Reverse (-)
30351417 .. 30356140
4724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024460.1

Sequence Viewer

Length: 1197 bp
ATGGAGACTCTATGCTTTACTCCTACATCTTCGCCACAACATCTCAAAAACCACCACACTTCATCATCAAACTCAGTCCCATCCCTCATCAGACCACTAGTTTTCAAAGACTATAACAATCCAAAGAAGAATATTAACCTAAGAAACCAACAATATTGCAAAGCTATATTAACAAACCCAGCTGAAGTGACCTCCTTCAAACCTTCCCCGCTGCCCAGTCCCTTCGAAACAACGTCGCTGCACGAACCCTCACCAGATCACTCCCATGAGTTAAGGAAGGTCCTCCCGGAGACATTGGAGTACGAGAGCGGCTTTCTCGGTTCCGTGCCGAAAAAGTTTGCCGGAGATGATGGTAACAGAATTCCCCAGACCATGATGAGCTACTTGACGAGGATTTTGACGACTAAGGTTTATGATGTGGCCATTGAATCACCACTGGACTACGCTTCAAAGCTCTCCGACAAGCTCGGCGTTTTCTCCTTTAAACTGCGAGGAGCTTATAATATGATGGCAAAGCTTTCAAGTGAACAACTAGACAGAGGTGTCATTTGCTCATCAGCAGGGAATCATGCTCAAGGAGTTGCACTATCTGCACGAAAACTAGGGTGCACTGCCGTCATTGTCATGCCTATCACAACTCCGGAAATCAAAGTTAAGATCATTGGTGTAGAGTTATCCGATGCAAATGCAATGGCACTGTCATTACATCACGGTGAGAGAGTTGTGCTTGAGCGACTTGGAGGATTTGCAGATGGCATGGCAGTCAAAAAGGTTGGGGAAGAAACTTTTCGATTGTGCAGGGAACTAATGGATGGAGTTGTTCTTGTTAATCGTGATGCCATTTGTGCATCCATAAAAGACATGTTTGAGCAAAAGCGGAGCATACTTGAACCGGCAGGGGCAGCAGCCATTGCTGGAGCTGAAGCATATTGCAAATATTATGGACTGAAGGGTGAAAATGTTGTGGCAATAACCAGTGGAGCAAACATGAATTTTGATAGACTAAGGTTAGTATCCCAACTAGCTGATGTAGGCCGGAGACGTGAAGGTTTCCTTGCAACATTTGTTCCAGAAAAGTCTGGAAGCTTCAAACATTTTTGTGAATTGGTGGGTCCAATGAATATCATCGAGATTAGCTACAGATTTGATGATAAAAAACAAGAAGGTCTTATCCTGTACAGGCAAGAAAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

398

Amino Acids

43.92

Weight (kDa)

8.49

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 158 - 218 4.5e-15 Pyridoxal-phosphate dependent enzyme
PALP PF00291 217 - 327 1.9e-15 Pyridoxal-phosphate dependent enzyme
Thr_dehydrat_C PF00585 342 - 394 3.6e-10 C-terminal regulatory domain of Threonine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AasI GACNNNNNNGTC 1 cut(s) 542
AccBSI CCGCTC 1 cut(s) 309
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 3 cut(s) 209, 309, 877
AcoI YGGCCR 1 cut(s) 420
AcsI RAATTY 2 cut(s) 360, 991
AcuI CTGAAG 3 cut(s) 204, 942, 968
AfaI GTAC 2 cut(s) 302, 1178
AflIII ACRYGT 1 cut(s) 861
AgsI TTSAA 7 cut(s) 106, 199, 428, 450, 522, 890, 1090
AhlI ACTAGT 1 cut(s) 97
AjiI CACGTC 1 cut(s) 1043
Alw21I GWGCWC 1 cut(s) 611
Alw26I GTCTC 2 cut(s) 284, 1033
Alw44I GTGCAC 1 cut(s) 607
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 2 cut(s) 420, 1033
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 4 cut(s) 211, 238, 902, 905
ApoI RAATTY 2 cut(s) 360, 991
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
Asp700I GAANNNNTTC 1 cut(s) 786
AspS9I GGNCC 2 cut(s) 280, 1112
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 4 cut(s) 243, 423, 725, 965
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 2 cut(s) 280, 1112
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 4 cut(s) 198, 225, 914, 917
BccI CCATC 5 cut(s) 88, 344, 502, 746, 806
BceAI ACGGC 1 cut(s) 599
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciVI GTATCC 1 cut(s) 1024
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 2 cut(s) 284, 1033
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 4 cut(s) 98, 533, 602, 1022
BfmI CTRYAG 1 cut(s) 1138
BfuI GTATCC 1 cut(s) 1024
BisI GCNGC 5 cut(s) 212, 239, 310, 903, 906
BlsI GCNGC 5 cut(s) 213, 240, 311, 904, 907
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 2 cut(s) 280, 1112
BmgBI CACGTC 1 cut(s) 1043
BmgT120I GGNCC 2 cut(s) 280, 1112
BmiI GGNNCC 2 cut(s) 322, 1113
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 3 cut(s) 670, 826, 857
BmuI ACTGGG 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 558, 749
BpuMI CCSGG 1 cut(s) 287
BsaWI WCCGGW 1 cut(s) 640
Bse118I RCCGGY 1 cut(s) 892
Bse1I ACTGG 3 cut(s) 216, 441, 975
Bse3DI GCAATG 2 cut(s) 696, 909
BseAI TCCGGA 1 cut(s) 640
BseGI GGATG 3 cut(s) 80, 817, 848
BseMI GCAATG 2 cut(s) 696, 909
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 3 cut(s) 216, 441, 975
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 4 cut(s) 198, 225, 914, 917
BseYI CCCAGC 1 cut(s) 178
BsgI GTGCAG 3 cut(s) 224, 576, 817
BshFI GGCC 2 cut(s) 422, 1035
BsiHKAI GWGCWC 1 cut(s) 611
BsiSI CCGG 5 cut(s) 287, 342, 641, 893, 1036
BslFI GGGAC 2 cut(s) 62, 204
BsmAI GTCTC 2 cut(s) 284, 1033
BsmBI CGTCTC 1 cut(s) 1033
BsmFI GGGAC 2 cut(s) 62, 204
BsnI GGCC 2 cut(s) 422, 1035
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 611
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 1 cut(s) 1176
Bsp143I GATC 2 cut(s) 256, 657
BspACI CCGC 3 cut(s) 209, 309, 877
BspANI GGCC 2 cut(s) 422, 1035
BspCNI CTCAG 1 cut(s) 86
BspEI TCCGGA 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 322, 1113
BspT104I TTCGAA 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 309
BsrDI GCAATG 2 cut(s) 696, 909
BsrFI RCCGGY 1 cut(s) 892
BsrGI TGTACA 1 cut(s) 1176
BsrI ACTGG 3 cut(s) 216, 441, 975
BssAI RCCGGY 1 cut(s) 892
BssMI GATC 2 cut(s) 256, 657
Bst4CI ACNGT 2 cut(s) 699, 713
BstAPI GCANNNNNTGC 2 cut(s) 590, 911
BstAUI TGTACA 1 cut(s) 1176
BstBI TTCGAA 1 cut(s) 225
BstDEI CTNAG 4 cut(s) 73, 140, 405, 1004
BstF5I GGATG 3 cut(s) 80, 817, 848
BstKTI GATC 2 cut(s) 259, 660
BstMAI GTCTC 2 cut(s) 284, 1033
BstMBI GATC 2 cut(s) 256, 657
BstMWI GCNNNNNNNGC 4 cut(s) 590, 845, 902, 911
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 1138
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 4 cut(s) 198, 225, 914, 917
BsuI GTATCC 1 cut(s) 1024
BsuRI GGCC 2 cut(s) 422, 1035
BtrI CACGTC 1 cut(s) 1043
BtsCI GGATG 3 cut(s) 80, 817, 848
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 4 cut(s) 434, 609, 695, 982
Cfr10I RCCGGY 1 cut(s) 892
Cfr13I GGNCC 2 cut(s) 280, 1112
Csp6I GTAC 2 cut(s) 301, 1177
CviAII CATG 7 cut(s) 266, 373, 569, 625, 757, 862, 988
CviQI GTAC 2 cut(s) 301, 1177
DdeI CTNAG 4 cut(s) 73, 140, 405, 1004
DpnI GATC 2 cut(s) 258, 659
DpnII GATC 2 cut(s) 256, 657
DraI TTTAAA 1 cut(s) 484
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
EaeI YGGCCR 1 cut(s) 420
Eco47I GGWCC 2 cut(s) 280, 1112
Eco57I CTGAAG 3 cut(s) 204, 942, 968
EcoO109I RGGNCCY 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 1033
FaeI CATG 7 cut(s) 269, 376, 572, 628, 760, 865, 991
FalI AAGNNNNNCTT 4 cut(s) 1038, 1070, 1152, 1184
FaqI GGGAC 2 cut(s) 62, 204
FatI CATG 7 cut(s) 265, 372, 568, 624, 756, 861, 987
FauI CCCGC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 212, 239, 310, 903, 906
FokI GGATG 3 cut(s) 67, 824, 835
Fsp4HI GCNGC 5 cut(s) 212, 239, 310, 903, 906
FspBI CTAG 4 cut(s) 98, 533, 602, 1022
GluI GCNGC 5 cut(s) 212, 239, 310, 903, 906
GsaI CCCAGC 1 cut(s) 182
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 2 cut(s) 422, 1035
HapII CCGG 5 cut(s) 287, 342, 641, 893, 1036
Hin1II CATG 7 cut(s) 269, 376, 572, 628, 760, 865, 991
HindIII AAGCTT 2 cut(s) 515, 1084
HinfI GANTC 3 cut(s) 7, 428, 565
HpaII CCGG 5 cut(s) 287, 342, 641, 893, 1036
HphI GGTGA 4 cut(s) 243, 423, 725, 965
Hpy166II GTNNAC 2 cut(s) 527, 609
Hpy188I TCNGA 3 cut(s) 92, 460, 679
Hpy188III TCNNGA 5 cut(s) 641, 833, 1070, 1080, 1129
Hpy8I GTNNAC 2 cut(s) 527, 609
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 7 cut(s) 205, 213, 232, 271, 943, 1040, 1157
HpyCH4III ACNGT 2 cut(s) 699, 713
HpyCH4IV ACGT 2 cut(s) 233, 1042
HpyF10VI GCNNNNNNNGC 4 cut(s) 590, 845, 902, 911
HpyF3I CTNAG 4 cut(s) 73, 140, 405, 1004
HpySE526I ACGT 2 cut(s) 233, 1042
Hsp92II CATG 7 cut(s) 269, 376, 572, 628, 760, 865, 991
Kpn2I TCCGGA 1 cut(s) 640
Kzo9I GATC 2 cut(s) 256, 657
LmnI GCTCC 4 cut(s) 494, 879, 917, 980
Lsp1109I GCAGC 4 cut(s) 198, 225, 914, 917
LweI GCATC 3 cut(s) 670, 826, 857
MaeI CTAG 4 cut(s) 98, 533, 602, 1022
MaeII ACGT 2 cut(s) 233, 1042
MaeIII GTNAC 2 cut(s) 187, 353
MalI GATC 2 cut(s) 258, 659
MbiI CCGCTC 1 cut(s) 309
MboI GATC 2 cut(s) 256, 657
MboII GAAGA 3 cut(s) 21, 139, 791
MhlI GDGCHC 1 cut(s) 611
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 3 cut(s) 360, 991, 1103
MluNI TGGCCA 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 8 cut(s) 95, 202, 259, 293, 384, 485, 533, 734
Mox20I TGGCCA 1 cut(s) 422
MroI TCCGGA 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 422
MseI TTAA 7 cut(s) 135, 170, 272, 483, 654, 828, 1195
MslI CAYNNNNRTG 4 cut(s) 264, 623, 711, 1098
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 2 cut(s) 182, 211
MspI CCGG 5 cut(s) 287, 342, 641, 893, 1036
MspR9I CCNGG 1 cut(s) 287
MwoI GCNNNNNNNGC 4 cut(s) 590, 845, 902, 911
NciI CCSGG 1 cut(s) 287
NdeII GATC 2 cut(s) 256, 657
NlaIII CATG 7 cut(s) 269, 376, 572, 628, 760, 865, 991
NlaIV GGNNCC 2 cut(s) 322, 1113
NmeAIII GCCGAG 1 cut(s) 447
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 865
NspV TTCGAA 1 cut(s) 225
PciI ACATGT 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 2 cut(s) 428, 565
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 5 cut(s) 213, 240, 311, 904, 907
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 861
PsiI TTATAA 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 322, 1113
PspPI GGNCC 2 cut(s) 280, 1112
PspPPI RGGWCCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 2 cut(s) 302, 1178
RsaNI GTAC 2 cut(s) 301, 1177
RseI CAYNNNNRTG 4 cut(s) 264, 623, 711, 1098
SaqAI TTAA 7 cut(s) 135, 170, 272, 483, 654, 828, 1195
SatI GCNGC 5 cut(s) 212, 239, 310, 903, 906
Sau3AI GATC 2 cut(s) 256, 657
Sau96I GGNCC 2 cut(s) 280, 1112
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 3 cut(s) 670, 826, 857
SfcI CTRYAG 1 cut(s) 1138
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 2 cut(s) 280, 1112
SmiMI CAYNNNNRTG 4 cut(s) 264, 623, 711, 1098
SmlI CTYRAG 2 cut(s) 573, 728
SmoI CTYRAG 2 cut(s) 573, 728
SpeI ACTAGT 1 cut(s) 97
Sse9I AATT 3 cut(s) 360, 991, 1103
SsiI CCGC 3 cut(s) 209, 309, 877
SspI AATATT 3 cut(s) 133, 155, 938
SspMI CTAG 4 cut(s) 98, 533, 602, 1022
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 699, 713
TaiI ACGT 2 cut(s) 236, 1045
TaqI TCGA 3 cut(s) 225, 790, 1128
TasI AATT 3 cut(s) 360, 991, 1103
TatI WGTACW 1 cut(s) 1176
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 2 cut(s) 428, 565
Tru1I TTAA 7 cut(s) 135, 170, 272, 483, 654, 828, 1195
Tru9I TTAA 7 cut(s) 135, 170, 272, 483, 654, 828, 1195
TscAI CASTG 4 cut(s) 441, 616, 702, 982
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 4 cut(s) 211, 238, 902, 905
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 51, 1004, 1133
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 4 cut(s) 441, 616, 702, 982
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 2 cut(s) 280, 1112
XapI RAATTY 2 cut(s) 360, 991
XceI RCATGY 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 4 cut(s) 98, 533, 602, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.