Rmu_sc0006749.1_g000006

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006749.1
OG0001514
Physical Location & Seq
Reverse (-)
24686 .. 33294
8609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006749.1_g000006.1.cds

Sequence Viewer

Length: 2808 bp
atggagactctatgctttactcctacatcttcactacaacttctcaaaaaccaccgcacttcatcatcaaccacagtcccatccctcatcagaccactagttttcaaagactataacaatccaaagaagaatattaacctcagaaaccaagaatgcaaagctataattacaaacccagctgaagtgacctccttcaaaccttccccgctgcccagtcccttcgaaacgacgccgctgcacgaaccctcacaatatcactcccatgagttaaggaaggtcctcccggagacattggagtacgagagcggctttctcggttccgtgccgaaaaagttcgccggcgatgatggtaacggaattccccagaccatgatgagctacttgacgaggattttgacgtctaaggtttatgatgtggccattgaatcaccactggactacgcttcaaagctctccgacaagctcggcgttcatctttggctcaagagagaggatctacaacccgttttctcctttaaactgcgaggagcttataatatgatggcaaagcttgaaagtgaacaactggacagaggtgtcatttgctcatcagcagggaatcatgctcaaggagttgcactatctgcacgaaaactagggtgcactgccgtcattgtcatgcctattacaactccggaaatcaaatggaaaacagtggagagtttaggtggaacagttgtgcttgtgggagagtcctatgatgaagctcaatcttacgcactacaaagggccaaagatgaaggcctcatatttgtacatccttttgatcacccagatgtgatagcaggtcagggaactgtaggaatggagatactacgccaaattcaaggcccattgcacgcaatctttgtgcctgttggtggtggtggactaattgccggcattgcttcctatgtcaagagtattcggccggaggttaagatcattggtgtagagttatccgatgcaaatgcaatggcactgtcattacatcacggtgagagagttgtgcttgagcgagttggaggatttgcagatggcatggcagtcaaaaaagttggggaagaaacttttcgattgtgccgggaactaatggatggagttgttcttgttaatcgtgatgccatttgtgcatccataaaagacatgtttgagcaaaaacggagcatacttgaaccggcaggggcagcagccattgctggagctgaagcatattgcaaatattatggactgaagggtgaaaatgttgtggcaataaccagtggagcaaacatgaattttgatagactgaggttggtatcccaactagctgatgttggtcggagacgtgaaggtttccttgcaacatttgttccagaaaagtgtggaagcttcaaacttttttgtgaattggtgggtccaatgaatatcatcgagattagctacagatttgatgataaaaaacaagaaggtcttatccttgtttcctttcacacaatttcagagttggaagggatgttggaccgtatgaagttggctcagcttcaaacaattaacctcacagataaagaactaatcaaagatcacatggaatttttggtgggaggtcgatcaaatgtcgaaaacgagattctttgccgccttttctttccagagagaccaggcgctctgatgaagttcttaaatgctttcagtcccctttggaacattaccttgttccattacagtggacagggtgaaactggtgcaaatgctttggtgggtttccaagtcgcaagctctgagattgacaagttcatggtgctctatgatgtgggtgatgggcagcctttggttcctgcaatgttcatatttggagactcagttgtggatgtgggtaataacaacaacctcttctccttaatcaaatccaacttccctccttatggaagagattttgtcaatcacaatcccaccggcaggttctgcaatggaaagcttgcttcagacttcactgctgaaaaccttggctttgcttctcaccaaccagcttatctcagcggagaagccacaggaaaaagcctcttgattggtgccaactttgcttcagctggctctggctattatgacgctacagcaaagttatttaatgcaatttcactgagccagcaggtggagtactacaaggaatatcaaaataaggtggtgggaattggaggaaaagtgaatgctacattaatcatagcgggtgcaatataccttgttagtgctgggagcagtgattttgttcagaactactacatcaatcctcttcttaacaaggtctatacgacagaccagttctctaatattctcatgcagtcctatgaaaacttcatacagaatctatatgccttgggagcacgcagaatcggagtgaccaccctgccaccacttggatgtctgccggcggccatcacaatatttggattaggcggcaacaagtgcgtggctaagcttaatggcgatgcagtctcatttaacaataagctaaatgccacatctcagtacttgcaaactaagctgcccggactcaagttggtcgtctttgacatctaccagcctctctacaaccttgtcacgaagccttcagagaatgggttctcagaggcgagaagggcttgttgtggatcagggttgctggaaacatcaatactatgcaatgccaagtccaccggcacatgcgccaatgcgtcagagtatgtgttctgggatggttttcacccttcggaggctgcaaacaaaattttagctgatgatttgctgagtagtggcatctctctcgtcttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

935

Amino Acids

102.06

Weight (kDa)

6.24

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AarI CACCTGC 1 cut(s) 2137
AasI GACNNNNNNGTC 1 cut(s) 575
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 3 cut(s) 815, 1947, 2137
AccB1I GGYRCC 1 cut(s) 2069
AccB7I CCANNNNNTGG 2 cut(s) 2149, 2411
AccBSI CCGCTC 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 9 cut(s) 55, 206, 233, 306, 1636, 2037, 2222, 2426, 2451
AclWI GGATC 2 cut(s) 501, 2653
AcoI YGGCCR 3 cut(s) 417, 947, 2427
AcsI RAATTY 5 cut(s) 357, 861, 1294, 1589, 2760
AcuI CTGAAG 6 cut(s) 201, 1245, 1271, 1965, 2067, 2589
AcyI GRCGYC 2 cut(s) 230, 398
AfaI GTAC 4 cut(s) 299, 795, 2156, 2525
AfiI CCNNNNNNNGG 6 cut(s) 899, 1922, 1956, 2149, 2411, 2746
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 9 cut(s) 106, 196, 425, 447, 554, 866, 1193, 1393, 1544
AhlI ACTAGT 1 cut(s) 97
AjiI CACGTC 1 cut(s) 1346
AjnI CCWGG 1 cut(s) 1657
Alw21I GWGCWC 3 cut(s) 644, 1803, 2380
Alw26I GTCTC 5 cut(s) 281, 1336, 1648, 1848, 2494
Alw44I GTGCAC 1 cut(s) 640
AlwI GGATC 2 cut(s) 501, 2653
AlwNI CAGNNNCTG 1 cut(s) 1962
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 6 cut(s) 417, 768, 781, 868, 947, 2427
ApaLI GTGCAC 1 cut(s) 640
ApeKI GCWGC 7 cut(s) 208, 235, 1205, 1208, 1822, 2539, 2750
ApoI RAATTY 5 cut(s) 357, 861, 1294, 1589, 2760
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AseI ATTAAT 1 cut(s) 2213
Asp700I GAANNNNTTC 3 cut(s) 332, 1089, 2615
AspLEI GCGC 2 cut(s) 1664, 2702
AspS9I GGNCC 5 cut(s) 277, 768, 869, 1415, 1519
AsuC2I CCSGG 3 cut(s) 284, 1103, 2544
AsuHPI GGTGA 8 cut(s) 420, 800, 1028, 1268, 1745, 1826, 2009, 2729
AsuII TTCGAA 1 cut(s) 222
AvaII GGWCC 3 cut(s) 277, 1415, 1519
BaeGI GKGCMC 1 cut(s) 644
BalI TGGCCA 1 cut(s) 419
BanI GGYRCC 1 cut(s) 2069
BbsI GAAGAC 1 cut(s) 2794
Bbv12I GWGCWC 3 cut(s) 644, 1803, 2380
BbvI GCAGC 7 cut(s) 195, 222, 1217, 1220, 1834, 2526, 2737
BccI CCATC 8 cut(s) 88, 341, 535, 1049, 1109, 1811, 2438, 2723
BceAI ACGGC 1 cut(s) 632
BcgI CGANNNNNNTGC 5 cut(s) 217, 251, 971, 1005, 2779
BciT130I CCWGG 1 cut(s) 1659
BciVI GTATCC 1 cut(s) 1327
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 3 cut(s) 284, 1103, 2544
BcoDI GTCTC 5 cut(s) 281, 1336, 1648, 1848, 2494
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 3 cut(s) 98, 635, 1325
BfmI CTRYAG 3 cut(s) 837, 1441, 2109
BfoI RGCGCY 1 cut(s) 1665
BfuAI ACCTGC 3 cut(s) 815, 1947, 2137
BfuI GTATCC 1 cut(s) 1327
BlpI GCTNAGC 2 cut(s) 1536, 2469
BmcAI AGTACT 2 cut(s) 2156, 2525
Bme1390I CCNGG 4 cut(s) 284, 1103, 1659, 2544
Bme18I GGWCC 3 cut(s) 277, 1415, 1519
BmgBI CACGTC 1 cut(s) 1346
BmgT120I GGNCC 5 cut(s) 277, 768, 869, 1415, 1519
BmiI GGNNCC 4 cut(s) 319, 1416, 1833, 2071
BmrFI CCNGG 4 cut(s) 284, 1103, 1659, 2544
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 5 cut(s) 973, 1129, 1160, 2473, 2799
BmuI ACTGGG 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 2794
BpmI CTGGAG 1 cut(s) 1239
Bpu1102I GCTNAGC 2 cut(s) 1536, 2469
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 4 cut(s) 467, 591, 1052, 2534
BpuMI CCSGG 3 cut(s) 284, 1103, 2544
BsaHI GRCGYC 2 cut(s) 230, 398
BsaI GGTCTC 1 cut(s) 1648
BsaJI CCNNGG 2 cut(s) 2002, 2370
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 6 cut(s) 1845, 1875, 1877, 1907, 2184, 2214
Bsc4I CCNNNNNNNGG 6 cut(s) 899, 1922, 1956, 2149, 2411, 2746
Bse118I RCCGGY 6 cut(s) 338, 917, 1195, 1952, 2422, 2690
Bse1I ACTGG 6 cut(s) 213, 438, 570, 1278, 1744, 2314
Bse3DI GCAATG 7 cut(s) 872, 921, 999, 1212, 1845, 1972, 2683
BseAI TCCGGA 1 cut(s) 673
BseBI CCWGG 1 cut(s) 1659
BseDI CCNNGG 2 cut(s) 2002, 2370
BseGI GGATG 8 cut(s) 80, 796, 1120, 1151, 1518, 1873, 2420, 2734
BseLI CCNNNNNNNGG 6 cut(s) 899, 1922, 1956, 2149, 2411, 2746
BseMI GCAATG 7 cut(s) 872, 921, 999, 1212, 1845, 1972, 2683
BseNI ACTGG 6 cut(s) 213, 438, 570, 1278, 1744, 2314
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 644
BseX3I CGGCCG 1 cut(s) 947
BseXI GCAGC 7 cut(s) 195, 222, 1217, 1220, 1834, 2526, 2737
BseYI CCCAGC 2 cut(s) 175, 2246
BsgI GTGCAG 2 cut(s) 221, 609
Bsh1285I CGRYCG 1 cut(s) 950
BshFI GGCC 6 cut(s) 419, 770, 783, 870, 949, 2429
BshNI GGYRCC 1 cut(s) 2069
BsiEI CGRYCG 1 cut(s) 950
BsiHKAI GWGCWC 3 cut(s) 644, 1803, 2380
BslFI GGGAC 3 cut(s) 62, 201, 1677
BslI CCNNNNNNNGG 6 cut(s) 899, 1922, 1956, 2149, 2411, 2746
BsmAI GTCTC 5 cut(s) 281, 1336, 1648, 1848, 2494
BsmBI CGTCTC 1 cut(s) 1336
BsmFI GGGAC 3 cut(s) 62, 201, 1677
BsmI GAATGC 2 cut(s) 158, 2209
BsnI GGCC 6 cut(s) 419, 770, 783, 870, 949, 2429
Bso31I GGTCTC 1 cut(s) 1648
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 3 cut(s) 644, 1803, 2380
Bsp13I TCCGGA 1 cut(s) 673
Bsp1407I TGTACA 1 cut(s) 793
Bsp143I GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
Bsp1720I GCTNAGC 2 cut(s) 1536, 2469
BspACI CCGC 9 cut(s) 55, 206, 233, 306, 1636, 2037, 2222, 2426, 2451
BspANI GGCC 6 cut(s) 419, 770, 783, 870, 949, 2429
BspEI TCCGGA 1 cut(s) 673
BspLI GGNNCC 4 cut(s) 319, 1416, 1833, 2071
BspMI ACCTGC 3 cut(s) 815, 1947, 2137
BspPI GGATC 2 cut(s) 501, 2653
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 2069
BspTNI GGTCTC 1 cut(s) 1648
BsrBI CCGCTC 1 cut(s) 306
BsrDI GCAATG 7 cut(s) 872, 921, 999, 1212, 1845, 1972, 2683
BsrFI RCCGGY 6 cut(s) 338, 917, 1195, 1952, 2422, 2690
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 6 cut(s) 213, 438, 570, 1278, 1744, 2314
BssAI RCCGGY 6 cut(s) 338, 917, 1195, 1952, 2422, 2690
BssECI CCNNGG 2 cut(s) 2002, 2370
BssMI GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
BssNI GRCGYC 2 cut(s) 230, 398
BssT1I CCWWGG 2 cut(s) 2002, 2370
Bst2UI CCWGG 1 cut(s) 1659
Bst4CI ACNGT 8 cut(s) 76, 694, 715, 838, 1002, 1016, 1523, 1724
Bst6I CTCTTC 3 cut(s) 1895, 1921, 2292
BstACI GRCGYC 2 cut(s) 230, 398
BstAPI GCANNNNNTGC 4 cut(s) 623, 1214, 1962, 2460
BstAUI TGTACA 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 9 cut(s) 340, 879, 919, 1774, 1977, 2089, 2144, 2380, 2424
BstF5I GGATG 8 cut(s) 80, 796, 1120, 1151, 1518, 1873, 2420, 2734
BstH2I RGCGCY 1 cut(s) 1665
BstHHI GCGC 2 cut(s) 1664, 2702
BstKTI GATC 6 cut(s) 496, 808, 963, 1582, 1610, 2648
BstMAI GTCTC 5 cut(s) 281, 1336, 1648, 1848, 2494
BstMBI GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
BstMCI CGRYCG 1 cut(s) 950
BstNI CCWGG 1 cut(s) 1659
BstNSI RCATGY 2 cut(s) 1168, 2700
BstSCI CCNGG 4 cut(s) 282, 1101, 1657, 2542
BstSFI CTRYAG 3 cut(s) 837, 1441, 2109
BstSLI GKGCMC 1 cut(s) 644
BstV1I GCAGC 7 cut(s) 195, 222, 1217, 1220, 1834, 2526, 2737
BstV2I GAAGAC 1 cut(s) 2794
BstX2I RGATCY 1 cut(s) 493
BstXI CCANNNNNNTGG 1 cut(s) 1724
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 1 cut(s) 947
BsuI GTATCC 1 cut(s) 1327
BsuRI GGCC 6 cut(s) 419, 770, 783, 870, 949, 2429
BtgZI GCGATG 2 cut(s) 357, 2496
BtrI CACGTC 1 cut(s) 1346
BtsCI GGATG 8 cut(s) 80, 796, 1120, 1151, 1518, 1873, 2420, 2734
BtsI GCAGTG 3 cut(s) 642, 1989, 2260
BtsIMutI CAGTG 9 cut(s) 431, 642, 699, 998, 1285, 1729, 1989, 2135, 2260
BveI ACCTGC 3 cut(s) 815, 1947, 2137
Cac8I GCNNGC 9 cut(s) 340, 879, 919, 1774, 1977, 2089, 2144, 2380, 2424
CaiI CAGNNNCTG 1 cut(s) 1962
CfoI GCGC 2 cut(s) 1664, 2702
Cfr10I RCCGGY 6 cut(s) 338, 917, 1195, 1952, 2422, 2690
Cfr13I GGNCC 5 cut(s) 277, 768, 869, 1415, 1519
CseI GACGC 3 cut(s) 238, 2114, 2697
Csp6I GTAC 4 cut(s) 298, 794, 2155, 2524
CviQI GTAC 4 cut(s) 298, 794, 2155, 2524
DpnI GATC 6 cut(s) 495, 807, 962, 1581, 1609, 2647
DpnII GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
DraI TTTAAA 1 cut(s) 517
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 3 cut(s) 417, 947, 2427
EagI CGGCCG 1 cut(s) 947
Eam1104I CTCTTC 3 cut(s) 1895, 1921, 2292
EarI CTCTTC 3 cut(s) 1895, 1921, 2292
EclXI CGGCCG 1 cut(s) 947
Eco130I CCWWGG 2 cut(s) 2002, 2370
Eco147I AGGCCT 1 cut(s) 783
Eco31I GGTCTC 1 cut(s) 1648
Eco47I GGWCC 3 cut(s) 277, 1415, 1519
Eco52I CGGCCG 1 cut(s) 947
Eco57I CTGAAG 6 cut(s) 201, 1245, 1271, 1965, 2067, 2589
EcoO109I RGGNCCY 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 1657
EcoT14I CCWWGG 2 cut(s) 2002, 2370
ErhI CCWWGG 2 cut(s) 2002, 2370
Esp3I CGTCTC 1 cut(s) 1336
FalI AAGNNNNNCTT 4 cut(s) 1341, 1373, 1455, 1487
FaqI GGGAC 3 cut(s) 62, 201, 1677
FauI CCCGC 2 cut(s) 213, 2215
FbaI TGATCA 1 cut(s) 805
FokI GGATG 8 cut(s) 67, 783, 1127, 1138, 1525, 1880, 2427, 2741
FspBI CTAG 3 cut(s) 98, 635, 1325
GlaI GCGC 2 cut(s) 1663, 2701
GsaI CCCAGC 2 cut(s) 179, 2250
GsuI CTGGAG 1 cut(s) 1239
HaeII RGCGCY 1 cut(s) 1665
HaeIII GGCC 6 cut(s) 419, 770, 783, 870, 949, 2429
HgaI GACGC 3 cut(s) 238, 2114, 2697
HhaI GCGC 2 cut(s) 1664, 2702
Hin1I GRCGYC 2 cut(s) 230, 398
Hin6I GCGC 2 cut(s) 1662, 2700
HinP1I GCGC 2 cut(s) 1662, 2700
HindIII AAGCTT 4 cut(s) 548, 1387, 1973, 2471
HinfI GANTC 9 cut(s) 7, 425, 598, 731, 1627, 1856, 2359, 2385, 2547
HphI GGTGA 8 cut(s) 420, 800, 1028, 1268, 1745, 1826, 2009, 2729
Hpy166II GTNNAC 5 cut(s) 560, 642, 908, 1727, 2688
Hpy188III TCNNGA 9 cut(s) 484, 674, 937, 1136, 1373, 1432, 1649, 2062, 2596
Hpy8I GTNNAC 5 cut(s) 560, 642, 908, 1727, 2688
Hpy99I CGWCG 1 cut(s) 232
HpyCH4III ACNGT 8 cut(s) 76, 694, 715, 838, 1002, 1016, 1523, 1724
HpyCH4IV ACGT 2 cut(s) 398, 1345
HpySE526I ACGT 2 cut(s) 398, 1345
Hsp92I GRCGYC 2 cut(s) 230, 398
HspAI GCGC 2 cut(s) 1662, 2700
Kpn2I TCCGGA 1 cut(s) 673
KroI GCCGGC 3 cut(s) 338, 917, 2422
KroNI GCCGGC 3 cut(s) 340, 919, 2424
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
LmnI GCTCC 6 cut(s) 527, 1182, 1220, 1283, 2250, 2375
Lsp1109I GCAGC 7 cut(s) 195, 222, 1217, 1220, 1834, 2526, 2737
LweI GCATC 5 cut(s) 973, 1129, 1160, 2473, 2799
MaeI CTAG 3 cut(s) 98, 635, 1325
MaeII ACGT 2 cut(s) 398, 1345
MaeIII GTNAC 4 cut(s) 184, 350, 2392, 2593
MalI GATC 6 cut(s) 495, 807, 962, 1581, 1609, 2647
MbiI CCGCTC 1 cut(s) 306
MboI GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
MboII GAAGA 7 cut(s) 21, 139, 1094, 1882, 1938, 2279, 2794
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 3 cut(s) 644, 1803, 2380
MlsI TGGCCA 1 cut(s) 419
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 3 cut(s) 740, 1850, 2541
MmeI TCCRAC 6 cut(s) 480, 1021, 1319, 1485, 1497, 1932
Mox20I TGGCCA 1 cut(s) 419
MreI CGCCGGCG 1 cut(s) 338
MroI TCCGGA 1 cut(s) 673
MroNI GCCGGC 3 cut(s) 338, 917, 2422
MroXI GAANNNNTTC 3 cut(s) 332, 1089, 2615
MscI TGGCCA 1 cut(s) 419
MslI CAYNNNNRTG 6 cut(s) 261, 656, 813, 1014, 1722, 2413
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 5 cut(s) 179, 208, 235, 2037, 2087
MspR9I CCNGG 4 cut(s) 284, 1103, 1659, 2544
Mva1269I GAATGC 2 cut(s) 158, 2209
MvaI CCWGG 1 cut(s) 1659
NaeI GCCGGC 3 cut(s) 340, 919, 2424
NciI CCSGG 3 cut(s) 284, 1103, 2544
NdeII GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
NgoMIV GCCGGC 3 cut(s) 338, 917, 2422
NlaIV GGNNCC 4 cut(s) 319, 1416, 1833, 2071
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 3 cut(s) 184, 2392, 2593
NspI RCATGY 2 cut(s) 1168, 2700
NspV TTCGAA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 2137
PceI AGGCCT 1 cut(s) 783
PciI ACATGT 1 cut(s) 1164
PctI GAATGC 2 cut(s) 158, 2209
PdiI GCCGGC 3 cut(s) 340, 919, 2424
PdmI GAANNNNTTC 3 cut(s) 332, 1089, 2615
PfeI GAWTC 5 cut(s) 425, 598, 1627, 2359, 2385
PflMI CCANNNNNTGG 2 cut(s) 2149, 2411
PfoI TCCNGGA 1 cut(s) 282
PleI GAGTC 3 cut(s) 739, 1850, 2541
PpsI GAGTC 3 cut(s) 739, 1850, 2541
PpuMI RGGWCCY 1 cut(s) 277
PscI ACATGT 1 cut(s) 1164
PshBI ATTAAT 1 cut(s) 2213
PsiI TTATAA 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 1657
PspFI CCCAGC 2 cut(s) 175, 2246
PspGI CCWGG 1 cut(s) 1657
PspN4I GGNNCC 4 cut(s) 319, 1416, 1833, 2071
PspPI GGNCC 5 cut(s) 277, 768, 869, 1415, 1519
PspPPI RGGWCCY 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 1962
PsuI RGATCY 1 cut(s) 493
PvuII CAGCTG 2 cut(s) 179, 2087
RsaI GTAC 4 cut(s) 299, 795, 2156, 2525
RsaNI GTAC 4 cut(s) 298, 794, 2155, 2524
RseI CAYNNNNRTG 6 cut(s) 261, 656, 813, 1014, 1722, 2413
Sau3AI GATC 6 cut(s) 493, 805, 960, 1579, 1607, 2645
Sau96I GGNCC 5 cut(s) 277, 768, 869, 1415, 1519
ScaI AGTACT 2 cut(s) 2156, 2525
SchI GAGTC 3 cut(s) 740, 1850, 2541
ScrFI CCNGG 4 cut(s) 284, 1103, 1659, 2544
SduI GDGCHC 3 cut(s) 644, 1803, 2380
SfaNI GCATC 5 cut(s) 973, 1129, 1160, 2473, 2799
SfcI CTRYAG 3 cut(s) 837, 1441, 2109
SfuI TTCGAA 1 cut(s) 222
SgrAI CRCCGGYG 1 cut(s) 338
SinI GGWCC 3 cut(s) 277, 1415, 1519
SmiMI CAYNNNNRTG 6 cut(s) 261, 656, 813, 1014, 1722, 2413
SmlI CTYRAG 4 cut(s) 482, 606, 1031, 2549
SmoI CTYRAG 4 cut(s) 482, 606, 1031, 2549
SpeI ACTAGT 1 cut(s) 97
SseBI AGGCCT 1 cut(s) 783
SsiI CCGC 9 cut(s) 55, 206, 233, 306, 1636, 2037, 2222, 2426, 2451
SspI AATATT 4 cut(s) 133, 1241, 2326, 2439
SspMI CTAG 3 cut(s) 98, 635, 1325
StuI AGGCCT 1 cut(s) 783
StyD4I CCNGG 4 cut(s) 282, 1101, 1657, 2542
StyI CCWWGG 2 cut(s) 2002, 2370
TaaI ACNGT 8 cut(s) 76, 694, 715, 838, 1002, 1016, 1523, 1724
TaiI ACGT 2 cut(s) 401, 1348
TaqI TCGA 5 cut(s) 222, 1093, 1431, 1606, 1617
TatI WGTACW 3 cut(s) 793, 2154, 2523
TauI GCSGC 5 cut(s) 235, 309, 1638, 2429, 2454
TfiI GAWTC 5 cut(s) 425, 598, 1627, 2359, 2385
TscAI CASTG 9 cut(s) 438, 649, 699, 1005, 1285, 1729, 1996, 2142, 2260
TseFI GTSAC 3 cut(s) 184, 2392, 2593
TseI GCWGC 7 cut(s) 208, 235, 1205, 1208, 1822, 2539, 2750
Tsp45I GTSAC 3 cut(s) 184, 2392, 2593
TspGWI ACGGA 3 cut(s) 310, 369, 1195
TspRI CASTG 9 cut(s) 438, 649, 699, 1005, 1285, 1729, 1996, 2142, 2260
Van91I CCANNNNNTGG 2 cut(s) 2149, 2411
VneI GTGCAC 1 cut(s) 640
VpaK11BI GGWCC 3 cut(s) 277, 1415, 1519
VspI ATTAAT 1 cut(s) 2213
XapI RAATTY 5 cut(s) 357, 861, 1294, 1589, 2760
XceI RCATGY 2 cut(s) 1168, 2700
XmnI GAANNNNTTC 3 cut(s) 332, 1089, 2615
XspI CTAG 3 cut(s) 98, 635, 1325
ZraI GACGTC 1 cut(s) 399
ZrmI AGTACT 2 cut(s) 2156, 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.