Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002416

Physical Location & Seq

Genomic Coordinates Forward (+)

38495343 .. 38498368

Gene Structure 3026 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1268900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1200 bp

ATGTTATTAAAACCCTGTAAAGTTGAGGAAAATCTTCCAAAACCCTATATATATGGTGTCCATTTCCTTTCTTCCCTCCAGTTTTCAATCACATTAATTTCTGGAATGGCTTCGTGGTTTAGTTTCATGATCTCCTCTGTTTCTCTTCTATCTCAGCTGGTTATACTGCAACCTTGTAAAGTGCATGGTAATAGTGATGCAGGCGAAGATAAACAACTAAAGCAATCAAATGGATGTGATTATTTTCAAGGAAGTTGGATAATTGATAATTCATATCCACTTTACGATTCATCAAAGTGTCCATTATTCATCGATCGAGAATTCGATTGCCTGAAGAATGGTAGACCTGATAAAGAATACCTCAAATATAGATGGAAACCGGCCAGTGACTGTGACCTTCCAAGATTCAATGGTGAGGACATGCTGAGGAGGGTAAAGGGAAAGAAGATCTTACTCGTCGGGGACTCTCTAAGCTTCAACCAATGGCAGTCACTTACATGCATGTTGCACACTGCAGTGCCCCAATCAAATTATACTCTAGATGACCCCACATTTTCTTTGCCTGAATATGGAGTTTCTGTTACACTATCTCGAAATACATTTCTAGTGGATCTAGTCACGACAGAGATGGGCAAGGTTCTCAAGCTTGACTCCATTGAAAATGGCAAAGCATGGAAAGGATACGACATGCTTATCTTCAACACTTGGCATTGGTGGCTCCACACAGGAGGCAAGCAACCATGGGACTACATTGAATCAGGAGGTAAAATACTCAAGGAAATTGACCGTTTGATTGCTTTTAGGGAGGGATTAACTACTTGGTCCAAGTGGGTGGACTCGAATGTTGATGCTAACAAGACCAAAGTTTTCTTTCAAGGCATTTCTCCAACCCATTACGATGGAAATGAATGGGACGACCCAAAGTCAACTTGCAATGGACAAACTCAACCTGTTAGTGGATCAATCTATCCAGGAGGTCCACCACCAGCAACAACTGTGGTCAAGGATGTCTTGACTACAATGTCAACGCCAATAACTTTGTTGGACATAACGTTACTATCGCAATTGCGAAAGGACGGGCACCCATCTGTATACGGCCTCGATGGAAAACATGGAAATGATTGTAGTCACTGGTGCCTTGCTGGTGTCCCAGATTCATGGAATGAACTATTGTATGCTATTCTTGTGACTACTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

400

Amino Acids

45.0

Weight (kDa)

5.74

Isoelectric Point (pI)

37.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	78 - 131	3.4e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	133 - 393	4e-90	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14850 AT3G14850
fragaria_vesca	FvH4_3g17810 FvH4_3g17820 FvH4_3g17830
malus_domestica	MD03G1246700.v1.1 MD11G1262000.v1.1 MD11G1268700.v1.1 MD11G1268800.v1.1 MD11G1268900.v1.1
prunus_persica	Prupe.4G157200_v2.0.a1 Prupe.4G157400_v2.0.a1 Prupe.4G157400_v2.0.a1 Prupe.4G157400_v2.0.a1 Prupe.4G157500_v2.0.a1 Prupe.4G157600_v2.0.a1
pyrus_communis	pycom03g19570 pycom11g23100 pycom11g23770
rosa_chinensis	RchiOBHm_Chr5g0029591 RchiOBHm_Chr5g0029601 RchiOBHm_Chr5g0029611
rosa_laevigata	RLG00000033185 RLG00000033186 RLG00000033187
rosa_multiflora	Rmu_sc0001608.1_g000005 Rmu_sc0001608.1_g000006
rosa_roxburghii	Rroxscaffold_1G00050310 Rroxscaffold_1G00050320
rosa_samantha	Rh5AG207700 Rh5BG206200 Rh5BG206300 Rh5BG206400 Rh5CG228300 Rh5CG228400 Rh5CG228500 Rh5DG210000 Rh5DG210100
rosa_wichuraiana	Rw5G018880 Rw5G018890 Rw5G018900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1021
AccB1I	GGYRCC	2 cut(s)	1080, 1134
AccI	GTMKAC	2 cut(s)	343, 1092
AclI	AACGTT	1 cut(s)	1052
AclWI	GGATC	2 cut(s)	618, 967
AcoI	YGGCCR	1 cut(s)	381
AcsI	RAATTY	1 cut(s)	320
AcuI	CTGAAG	1 cut(s)	353
AfiI	CCNNNNNNNGG	2 cut(s)	569, 956
AgsI	TTSAA	8 cut(s)	87, 248, 409, 478, 659, 700, 755, 875
AhdI	GACNNNNNGTC	1 cut(s)	922
AjnI	CCWGG	1 cut(s)	970
AleI	CACNNNNGTG	1 cut(s)	515
AluBI	AGCT	3 cut(s)	157, 474, 646
AluI	AGCT	3 cut(s)	157, 474, 646
AlwI	GGATC	2 cut(s)	618, 967
AlwNI	CAGNNNCTG	1 cut(s)	390
AoxI	GGCC	2 cut(s)	381, 1096
ApoI	RAATTY	1 cut(s)	320
AseI	ATTAAT	1 cut(s)	95
Asp700I	GAANNNNTTC	2 cut(s)	33, 109
AspS9I	GGNCC	2 cut(s)	822, 977
AsuHPI	GGTGA	1 cut(s)	425
AvaII	GGWCC	2 cut(s)	822, 977
BaeGI	GKGCMC	2 cut(s)	522, 1083
BanI	GGYRCC	2 cut(s)	1080, 1134
BbvCI	CCTCAGC	1 cut(s)	425
BccI	CCATC	5 cut(s)	366, 622, 893, 1093, 1097
BceAI	ACGGC	1 cut(s)	1111
BciT130I	CCWGG	1 cut(s)	972
BciVI	GTATCC	1 cut(s)	674
BfaI	CTAG	4 cut(s)	539, 605, 614, 1198
BfmI	CTRYAG	1 cut(s)	513
BfuI	GTATCC	1 cut(s)	674
BglII	AGATCT	1 cut(s)	447
Bme1390I	CCNGG	1 cut(s)	972
Bme18I	GGWCC	2 cut(s)	822, 977
BmeRI	GACNNNNNGTC	1 cut(s)	922
BmgT120I	GGNCC	2 cut(s)	822, 977
BmiI	GGNNCC	3 cut(s)	719, 1082, 1136
BmrFI	CCNGG	1 cut(s)	972
BmsI	GCATC	2 cut(s)	187, 838
BpmI	CTGGAG	1 cut(s)	62
Bpu10I	CCTNAGC	1 cut(s)	425
BpuEI	CTTGAG	2 cut(s)	626, 758
Bsa29I	ATCGAT	1 cut(s)	312
BsaJI	CCNNGG	1 cut(s)	740
BsaXI	ACNNNNNCTCC	2 cut(s)	564, 594
Bsc4I	CCNNNNNNNGG	2 cut(s)	569, 956
Bse118I	RCCGGY	1 cut(s)	379
Bse1I	ACTGG	3 cut(s)	79, 384, 1136
Bse3DI	GCAATG	1 cut(s)	940
BseBI	CCWGG	1 cut(s)	972
BseCI	ATCGAT	1 cut(s)	312
BseDI	CCNNGG	1 cut(s)	740
BseGI	GGATG	2 cut(s)	239, 1012
BseLI	CCNNNNNNNGG	2 cut(s)	569, 956
BseMI	GCAATG	1 cut(s)	940
BseMII	CTCAG	2 cut(s)	167, 416
BseNI	ACTGG	3 cut(s)	79, 384, 1136
BseRI	GAGGAG	2 cut(s)	124, 442
BseSI	GKGCMC	2 cut(s)	522, 1083
Bsh1285I	CGRYCG	1 cut(s)	316
BshFI	GGCC	2 cut(s)	383, 1098
BshNI	GGYRCC	2 cut(s)	1080, 1134
BshVI	ATCGAT	1 cut(s)	312
BsiEI	CGRYCG	1 cut(s)	316
BsiSI	CCGG	1 cut(s)	380
BslFI	GGGAC	4 cut(s)	476, 758, 926, 1133
BslI	CCNNNNNNNGG	2 cut(s)	569, 956
BsmFI	GGGAC	4 cut(s)	476, 758, 926, 1133
BsnI	GGCC	2 cut(s)	383, 1098
Bsp1286I	GDGCHC	2 cut(s)	522, 1083
Bsp143I	GATC	5 cut(s)	129, 313, 447, 610, 959
Bsp19I	CCATGG	1 cut(s)	740
BspANI	GGCC	2 cut(s)	383, 1098
BspCNI	CTCAG	2 cut(s)	166, 417
BspDI	ATCGAT	1 cut(s)	312
BspHI	TCATGA	1 cut(s)	126
BspLI	GGNNCC	3 cut(s)	719, 1082, 1136
BspMAI	CTGCAG	1 cut(s)	517
BspPI	GGATC	2 cut(s)	618, 967
BspT107I	GGYRCC	2 cut(s)	1080, 1134
BsrDI	GCAATG	1 cut(s)	940
BsrFI	RCCGGY	1 cut(s)	379
BsrI	ACTGG	3 cut(s)	79, 384, 1136
BssAI	RCCGGY	1 cut(s)	379
BssECI	CCNNGG	1 cut(s)	740
BssMI	GATC	5 cut(s)	129, 313, 447, 610, 959
BssNAI	GTATAC	1 cut(s)	1093
BssT1I	CCWWGG	1 cut(s)	740
Bst1107I	GTATAC	1 cut(s)	1093
Bst2UI	CCWGG	1 cut(s)	972
Bst4CI	ACNGT	3 cut(s)	392, 788, 997
Bst6I	CTCTTC	1 cut(s)	150
BstC8I	GCNNGC	2 cut(s)	202, 734
BstDEI	CTNAG	3 cut(s)	153, 425, 470
BstDSI	CCRYGG	1 cut(s)	740
BstF5I	GGATG	2 cut(s)	239, 1012
BstKTI	GATC	5 cut(s)	132, 316, 450, 613, 962
BstMBI	GATC	5 cut(s)	129, 313, 447, 610, 959
BstMCI	CGRYCG	1 cut(s)	316
BstMWI	GCNNNNNNNGC	1 cut(s)	715
BstNI	CCWGG	1 cut(s)	972
BstNSI	RCATGY	4 cut(s)	424, 501, 505, 691
BstSCI	CCNGG	1 cut(s)	970
BstSFI	CTRYAG	1 cut(s)	513
BstSLI	GKGCMC	2 cut(s)	522, 1083
BstX2I	RGATCY	2 cut(s)	447, 610
BstXI	CCANNNNNNTGG	3 cut(s)	832, 899, 1158
BstYI	RGATCY	2 cut(s)	447, 610
BstZ17I	GTATAC	1 cut(s)	1093
Bsu15I	ATCGAT	1 cut(s)	312
BsuI	GTATCC	1 cut(s)	674
BsuRI	GGCC	2 cut(s)	383, 1098
BsuTUI	ATCGAT	1 cut(s)	312
BtgI	CCRYGG	1 cut(s)	740
BtsCI	GGATG	2 cut(s)	239, 1012
BtsI	GCAGTG	2 cut(s)	510, 522
BtsIMutI	CAGTG	4 cut(s)	391, 510, 522, 1129
Cac8I	GCNNGC	2 cut(s)	202, 734
CaiI	CAGNNNCTG	1 cut(s)	390
CciI	TCATGA	1 cut(s)	126
Cfr10I	RCCGGY	1 cut(s)	379
Cfr13I	GGNCC	2 cut(s)	822, 977
ClaI	ATCGAT	1 cut(s)	312
CspCI	CAANNNNNGTGG	2 cut(s)	978, 1013
CviJI	RGCY	7 cut(s)	110, 157, 383, 474, 646, 718, 1098
CviKI_1	RGCY	7 cut(s)	110, 157, 383, 474, 646, 718, 1098
DdeI	CTNAG	3 cut(s)	153, 425, 470
DpnI	GATC	5 cut(s)	131, 315, 449, 612, 961
DpnII	GATC	5 cut(s)	129, 313, 447, 610, 959
DrdI	GACNNNNNNGTC	1 cut(s)	1021
DriI	GACNNNNNGTC	1 cut(s)	922
DseDI	GACNNNNNNGTC	1 cut(s)	1021
EaeI	YGGCCR	1 cut(s)	381
Eam1104I	CTCTTC	1 cut(s)	150
Eam1105I	GACNNNNNGTC	1 cut(s)	922
EarI	CTCTTC	1 cut(s)	150
Eco130I	CCWWGG	1 cut(s)	740
Eco47I	GGWCC	2 cut(s)	822, 977
Eco57I	CTGAAG	1 cut(s)	353
EcoRI	GAATTC	1 cut(s)	320
EcoRII	CCWGG	1 cut(s)	970
EcoT14I	CCWWGG	1 cut(s)	740
EcoT22I	ATGCAT	1 cut(s)	503
ErhI	CCWWGG	1 cut(s)	740
FalI	AAGNNNNNCTT	4 cut(s)	434, 466, 995, 1027
FaqI	GGGAC	4 cut(s)	476, 758, 926, 1133
FblI	GTMKAC	2 cut(s)	343, 1092
FokI	GGATG	2 cut(s)	246, 1019
FspBI	CTAG	4 cut(s)	539, 605, 614, 1198
GsuI	CTGGAG	1 cut(s)	62
HaeIII	GGCC	2 cut(s)	383, 1098
HapII	CCGG	1 cut(s)	380
HincII	GTYRAC	2 cut(s)	927, 1026
HindII	GTYRAC	2 cut(s)	927, 1026
HindIII	AAGCTT	2 cut(s)	472, 644
HinfI	GANTC	7 cut(s)	287, 405, 464, 650, 755, 836, 1154
HpaII	CCGG	1 cut(s)	380
HphI	GGTGA	1 cut(s)	425
Hpy166II	GTNNAC	6 cut(s)	344, 835, 927, 980, 1026, 1093
Hpy188III	TCNNGA	8 cut(s)	102, 127, 317, 539, 591, 619, 759, 1012
Hpy8I	GTNNAC	6 cut(s)	344, 835, 927, 980, 1026, 1093
Hpy99I	CGWCG	1 cut(s)	461
HpyAV	CCTTC	1 cut(s)	407
HpyCH4III	ACNGT	3 cut(s)	392, 788, 997
HpyCH4IV	ACGT	1 cut(s)	1052
HpyCH4V	TGCA	7 cut(s)	169, 184, 200, 501, 508, 515, 933
HpyF10VI	GCNNNNNNNGC	1 cut(s)	715
HpyF3I	CTNAG	3 cut(s)	153, 425, 470
HpySE526I	ACGT	1 cut(s)	1052
Kzo9I	GATC	5 cut(s)	129, 313, 447, 610, 959
LmnI	GCTCC	1 cut(s)	723
LweI	GCATC	2 cut(s)	187, 838
MaeI	CTAG	4 cut(s)	539, 605, 614, 1198
MaeII	ACGT	1 cut(s)	1052
MaeIII	GTNAC	8 cut(s)	386, 392, 489, 580, 616, 1053, 1127, 1186
MalI	GATC	5 cut(s)	131, 315, 449, 612, 961
MboI	GATC	5 cut(s)	129, 313, 447, 610, 959
MboII	GAAGA	7 cut(s)	26, 63, 137, 218, 346, 457, 688
MfeI	CAATTG	1 cut(s)	1064
MflI	RGATCY	2 cut(s)	447, 610
MhlI	GDGCHC	2 cut(s)	522, 1083
MluCI	AATT	7 cut(s)	96, 261, 268, 320, 529, 780, 1064
MlyI	GAGTC	3 cut(s)	458, 644, 830
MmeI	TCCRAC	3 cut(s)	236, 911, 1023
Mph1103I	ATGCAT	1 cut(s)	503
MroXI	GAANNNNTTC	2 cut(s)	33, 109
MseI	TTAA	3 cut(s)	8, 95, 812
MslI	CAYNNNNRTG	5 cut(s)	295, 496, 515, 897, 1116
MspA1I	CMGCKG	1 cut(s)	157
MspI	CCGG	1 cut(s)	380
MspR9I	CCNGG	1 cut(s)	972
MunI	CAATTG	1 cut(s)	1064
MvaI	CCWGG	1 cut(s)	972
MwoI	GCNNNNNNNGC	1 cut(s)	715
NcoI	CCATGG	1 cut(s)	740
NdeII	GATC	5 cut(s)	129, 313, 447, 610, 959
NlaIV	GGNNCC	3 cut(s)	719, 1082, 1136
NmuCI	GTSAC	6 cut(s)	386, 392, 489, 616, 1127, 1186
NsiI	ATGCAT	1 cut(s)	503
NspI	RCATGY	4 cut(s)	424, 501, 505, 691
OliI	CACNNNNGTG	1 cut(s)	515
PagI	TCATGA	1 cut(s)	126
PdmI	GAANNNNTTC	2 cut(s)	33, 109
PfeI	GAWTC	4 cut(s)	287, 405, 755, 1154
PfoI	TCCNGGA	1 cut(s)	970
Ple19I	CGATCG	1 cut(s)	316
PleI	GAGTC	3 cut(s)	458, 644, 830
PpsI	GAGTC	3 cut(s)	458, 644, 830
PshBI	ATTAAT	1 cut(s)	95
Psp1406I	AACGTT	1 cut(s)	1052
Psp6I	CCWGG	1 cut(s)	970
PspGI	CCWGG	1 cut(s)	970
PspN4I	GGNNCC	3 cut(s)	719, 1082, 1136
PspPI	GGNCC	2 cut(s)	822, 977
PstI	CTGCAG	1 cut(s)	517
PstNI	CAGNNNCTG	1 cut(s)	390
PsuI	RGATCY	2 cut(s)	447, 610
PvuI	CGATCG	1 cut(s)	316
PvuII	CAGCTG	1 cut(s)	157
RseI	CAYNNNNRTG	5 cut(s)	295, 496, 515, 897, 1116
SaqAI	TTAA	3 cut(s)	8, 95, 812
Sau3AI	GATC	5 cut(s)	129, 313, 447, 610, 959
Sau96I	GGNCC	2 cut(s)	822, 977
SchI	GAGTC	3 cut(s)	458, 644, 830
ScrFI	CCNGG	1 cut(s)	972
SduI	GDGCHC	2 cut(s)	522, 1083
SfaNI	GCATC	2 cut(s)	187, 838
SfcI	CTRYAG	1 cut(s)	513
SinI	GGWCC	2 cut(s)	822, 977
SmiMI	CAYNNNNRTG	5 cut(s)	295, 496, 515, 897, 1116
SmlI	CTYRAG	2 cut(s)	641, 773
SmoI	CTYRAG	2 cut(s)	641, 773
Sse9I	AATT	7 cut(s)	96, 261, 268, 320, 529, 780, 1064
SspMI	CTAG	4 cut(s)	539, 605, 614, 1198
StyD4I	CCNGG	1 cut(s)	970
StyI	CCWWGG	1 cut(s)	740
TaaI	ACNGT	3 cut(s)	392, 788, 997
TaiI	ACGT	1 cut(s)	1055
TaqI	TCGA	6 cut(s)	312, 316, 324, 592, 839, 1101
TasI	AATT	7 cut(s)	96, 261, 268, 320, 529, 780, 1064
TfiI	GAWTC	4 cut(s)	287, 405, 755, 1154
Tru1I	TTAA	3 cut(s)	8, 95, 812
Tru9I	TTAA	3 cut(s)	8, 95, 812
TscAI	CASTG	4 cut(s)	391, 517, 522, 1136
TseFI	GTSAC	6 cut(s)	386, 392, 489, 616, 1127, 1186
Tsp45I	GTSAC	6 cut(s)	386, 392, 489, 616, 1127, 1186
TspDTI	ATGAA	7 cut(s)	115, 261, 279, 298, 921, 1146, 1179
TspRI	CASTG	4 cut(s)	391, 517, 522, 1136
VpaK11BI	GGWCC	2 cut(s)	822, 977
VspI	ATTAAT	1 cut(s)	95
XapI	RAATTY	1 cut(s)	320
XbaI	TCTAGA	1 cut(s)	538
XceI	RCATGY	4 cut(s)	424, 501, 505, 691
XmiI	GTMKAC	2 cut(s)	343, 1092
XmnI	GAANNNNTTC	2 cut(s)	33, 109
XspI	CTAG	4 cut(s)	539, 605, 614, 1198
Zsp2I	ATGCAT	1 cut(s)	503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.