MD12G1059500.v1.1

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001646
Physical Location & Seq
Reverse (-)
6925201 .. 6928074
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1059500.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCCACGACTGTAGCACCCGGATCACACATGGCAGCGGTGAAGCCTTGCATGTCATCCTCTCAAAGAACTTATATGTCATGCACGGCAAAGTTTGGTACAAACAGCGAGGTGGGAGTTTGGAGAAGGCTTAAGAGTTCTTCTCACATCTCAGCAACACGGTCATTCTCCCAGAGCTTGAAATCAGGGCCTGTGAAATCCGTAAAGGTTGTCACCAGAGCAATGTCAGGAGCAGCTGAGAACACTCCACTGCCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATAGCTGGTGTGGCTGATGACCATGGATATGGATGGGCAATATCAAAAGCTCTTGCTGCTGCGGGAGCTGAAATTCTAGTTGGTACATGGGTGCCGGCACTTAACATTTTTGAATCCAGTTTAAGGCGTGGAAAGTTTGATGAATCACGCATACTGCCAGATGGCTCTTTGATGGAAATTACCAAGGTTTACCCATTGGATGCAGTTTTTGACAGTCCCGAGGATGTTCCTGAAGATATAAAAACCAATAAACGCTACTTGGGGTCCTCAAATTGGACTGTTAAGGAGGTGGTTGAATCTGTAAAGCAGGATTTTGGCAGCATTGACATCCTTGTCCACTCACTTGCCAATGGACCAGAGGTTACCAAACCTCTTTTGGAGACGTCAAGATATGGATATCTTGCAGCCATCTCTGCATCAAGTTACTCATATGTTTCTTTACTCAAGCATTTTGCTCCAATAATGAACCCAGGCACGGCTCTTATTTATTTTTCCTCCCCGCTTTCTCCCCTCTTCATTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

272

Amino Acids

29.2

Weight (kDa)

9.11

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 263 1.5e-12 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 623
AatII GACGTC 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 382
AciI CCGC 3 cut(s) 38, 353, 791
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 2 cut(s) 100, 376
AfiI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 766
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 3 cut(s) 181, 404, 587
AjnI CCWGG 2 cut(s) 253, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 5 cut(s) 177, 236, 296, 341, 359
AluI AGCT 5 cut(s) 177, 236, 296, 341, 359
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 3, 188
ApeKI GCWGC 6 cut(s) 35, 233, 347, 350, 609, 695
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 188, 555, 644
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 52, 205
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 2 cut(s) 555, 644
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbvI GCAGC 6 cut(s) 47, 245, 334, 337, 621, 707
BccI CCATC 4 cut(s) 318, 446, 457, 707
BceAI ACGGC 2 cut(s) 102, 783
BciT130I CCWGG 2 cut(s) 255, 762
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 6 cut(s) 36, 234, 348, 351, 610, 696
BlsI GCNGC 6 cut(s) 37, 235, 349, 352, 611, 697
Bme1390I CCNGG 3 cut(s) 21, 255, 762
Bme18I GGWCC 2 cut(s) 555, 644
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 188, 555, 644
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 3 cut(s) 21, 255, 762
BmsI GCATC 2 cut(s) 481, 716
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpuEI CTTGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 4 cut(s) 313, 474, 510, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 414, 564, 766
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 2 cut(s) 255, 762
BseDI CCNNGG 4 cut(s) 313, 474, 510, 760
BseGI GGATG 5 cut(s) 56, 329, 496, 520, 618
BseLI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 766
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 165, 228
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 6 cut(s) 47, 245, 334, 337, 621, 707
BshFI GGCC 2 cut(s) 5, 190
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 21, 386
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 766
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 2 cut(s) 5, 190
BsoBI CYCGRG 1 cut(s) 509
Bsp143I GATC 1 cut(s) 23
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 3 cut(s) 38, 353, 791
BspANI GGCC 2 cut(s) 5, 190
BspCNI CTCAG 2 cut(s) 164, 229
BspLI GGNNCC 2 cut(s) 384, 556
BspPI GGATC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 313, 474, 510, 760
BssMI GATC 1 cut(s) 23
BssNI GRCGYC 1 cut(s) 674
BssT1I CCWWGG 2 cut(s) 313, 474
Bst2UI CCWGG 2 cut(s) 255, 762
Bst4CI ACNGT 4 cut(s) 13, 162, 506, 571
Bst6I CTCTTC 1 cut(s) 809
BstACI GRCGYC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 151, 237
BstDSI CCRYGG 1 cut(s) 313
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 5 cut(s) 56, 329, 496, 520, 618
BstKTI GATC 1 cut(s) 26
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 1 cut(s) 23
BstMWI GCNNNNNNNGC 6 cut(s) 259, 293, 302, 347, 356, 704
BstNI CCWGG 2 cut(s) 255, 762
BstNSI RCATGY 1 cut(s) 55
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 19, 253, 760
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 6 cut(s) 47, 245, 334, 337, 621, 707
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuRI GGCC 2 cut(s) 5, 190
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 5 cut(s) 56, 329, 496, 520, 618
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 188, 555, 644
Csp6I GTAC 2 cut(s) 99, 375
CviAII CATG 5 cut(s) 31, 52, 81, 314, 378
CviQI GTAC 2 cut(s) 99, 375
DdeI CTNAG 2 cut(s) 151, 237
DpnI GATC 1 cut(s) 25
DpnII GATC 1 cut(s) 23
DrdI GACNNNNNNGTC 1 cut(s) 623
DseDI GACNNNNNNGTC 1 cut(s) 623
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Eco130I CCWWGG 2 cut(s) 313, 474
Eco32I GATATC 1 cut(s) 689
Eco47I GGWCC 2 cut(s) 555, 644
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 253, 760
EcoRV GATATC 1 cut(s) 689
EcoT14I CCWWGG 2 cut(s) 313, 474
ErhI CCWWGG 2 cut(s) 313, 474
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 5 cut(s) 34, 55, 84, 317, 381
FaqI GGGAC 1 cut(s) 492
FatI CATG 5 cut(s) 30, 51, 80, 313, 377
FauI CCCGC 2 cut(s) 346, 798
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 6 cut(s) 36, 234, 348, 351, 610, 696
FokI GGATG 5 cut(s) 43, 336, 503, 527, 605
Fsp4HI GCNGC 6 cut(s) 36, 234, 348, 351, 610, 696
FspBI CTAG 1 cut(s) 368
GluI GCNGC 6 cut(s) 36, 234, 348, 351, 610, 696
HaeIII GGCC 2 cut(s) 5, 190
HapII CCGG 2 cut(s) 21, 386
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 5 cut(s) 34, 55, 84, 317, 381
HinfI GANTC 3 cut(s) 404, 434, 587
HpaII CCGG 2 cut(s) 21, 386
HphI GGTGA 2 cut(s) 52, 205
Hpy166II GTNNAC 2 cut(s) 481, 628
Hpy188III TCNNGA 4 cut(s) 228, 509, 521, 678
Hpy8I GTNNAC 2 cut(s) 481, 628
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 4 cut(s) 13, 162, 506, 571
HpyCH4IV ACGT 1 cut(s) 674
HpyCH4V TGCA 5 cut(s) 51, 84, 494, 695, 707
HpyF10VI GCNNNNNNNGC 6 cut(s) 259, 293, 302, 347, 356, 704
HpyF3I CTNAG 2 cut(s) 151, 237
HpySE526I ACGT 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 5 cut(s) 34, 55, 84, 317, 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 23
LmnI GCTCC 3 cut(s) 230, 356, 751
Lsp1109I GCAGC 6 cut(s) 47, 245, 334, 337, 621, 707
LweI GCATC 2 cut(s) 481, 716
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 3 cut(s) 211, 652, 713
MalI GATC 1 cut(s) 25
MboI GATC 1 cut(s) 23
MboII GAAGA 3 cut(s) 132, 536, 796
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 363, 468, 562
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 132, 393, 413, 573, 814
MslI CAYNNNNRTG 1 cut(s) 318
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 38, 236
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 2 cut(s) 21, 386
MspR9I CCNGG 3 cut(s) 21, 255, 762
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 2 cut(s) 255, 762
MwoI GCNNNNNNNGC 6 cut(s) 259, 293, 302, 347, 356, 704
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 21
NcoI CCATGG 1 cut(s) 313
NdeI CATATG 1 cut(s) 721
NdeII GATC 1 cut(s) 23
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 34, 55, 84, 317, 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 211
NspI RCATGY 1 cut(s) 55
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 404, 434, 587
PkrI GCNGC 6 cut(s) 37, 235, 349, 352, 611, 697
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 253, 760
PspEI GGTNACC 1 cut(s) 652
PspGI CCWGG 2 cut(s) 253, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 3 cut(s) 188, 555, 644
PspPPI RGGWCCY 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 100, 376
RsaNI GTAC 2 cut(s) 99, 375
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 5 cut(s) 132, 393, 413, 573, 814
SatI GCNGC 6 cut(s) 36, 234, 348, 351, 610, 696
Sau3AI GATC 1 cut(s) 23
Sau96I GGNCC 3 cut(s) 188, 555, 644
ScrFI CCNGG 3 cut(s) 21, 255, 762
SfaNI GCATC 2 cut(s) 481, 716
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 2 cut(s) 555, 644
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 2 cut(s) 131, 734
SmoI CTYRAG 2 cut(s) 131, 734
Sse9I AATT 3 cut(s) 363, 468, 562
SsiI CCGC 3 cut(s) 38, 353, 791
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 19, 253, 760
StyI CCWWGG 2 cut(s) 313, 474
TaaI ACNGT 4 cut(s) 13, 162, 506, 571
TaiI ACGT 1 cut(s) 677
TasI AATT 3 cut(s) 363, 468, 562
TfiI GAWTC 3 cut(s) 404, 434, 587
Tru1I TTAA 5 cut(s) 132, 393, 413, 573, 814
Tru9I TTAA 5 cut(s) 132, 393, 413, 573, 814
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 6 cut(s) 35, 233, 347, 350, 609, 695
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 4 cut(s) 280, 447, 770, 796
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 255
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 555, 644
XapI RAATTY 1 cut(s) 363
XceI RCATGY 1 cut(s) 55
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XspI CTAG 1 cut(s) 368
ZraI GACGTC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.