Prupe.7G076200_v2.0.a1

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001646
Physical Location & Seq
Reverse (-)
11296473 .. 11302121
5649 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G076200.2

Sequence Viewer

Length: 1179 bp
ATGGCCACGACTACAACTCCTGGATCCCACATGGCAGCAGTTAATCCTTCCATATCCTCCTCTCGAAGAACTTATACTTCAGGCACAGCAAATTTTGGTACAAACAGTGAAGTGGGAGTTTGGAGAAGGCTTAAAAGTTCTTCTCACATCTCAGCAACGCAGCCATTCTTCCAGAGCTTCAAATCAGGGCCTACGAAATCTGTAAAGGCTGTCACCAGAGCAATGTCAGGAGCAGCTGAGAACAGTCCGTTGCCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGAGTGGCTGATGATCATGGATATGGATGGGCAATAGCAAAATCTCTTGCTGCTGCTGGGGCTGAAATTCTAGTTGGTACTTGGGTGCCGGCACTTAACATTTTCGAATCCAGTTTAAGGCGTGGGAAGTTTGATGAGTCGCGCAAATTGCCAGATGGCTCTTTAATGGAAATTACCAAGGTTTACCCATTGGACGCGGTTTATGACAATCCTGAGGATGTCCCTGAAGATGTAAAATCCAACAAGCGCTACTTGGGGTCCTCTAATTGGACTGTGAAGGAGGTTGTTGAATCTGTAAAACAGGATTTTGGCAGCATTGATATCCTTGTCCACTCACTTGCTAATGGACCAGAGGTTACTAAACCTCTTTTGGAGACATCAAGATATGGGTATCTTGCAGCAATCTCTGCATCGAGTTACTCATATGTTTCTTTACTCAAGCACTTTGCTCCAATAATTAACCCAGGTGGTGCTTCAATCTCTCTAACGTACATTGCGTCAGAAAGGATCATTCCGGGGTATGGTGGAGGCATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGCGTGCTGGCCTTCGAAGCTGGAAGAAAACACAACGTTAGAGTCAACACAATATCTGCTGGACCATTAAGAAGTCGTGCGGCAAAAGCAATTGGGTTCATTGATATGATGATTGATTACTCATCGGAAAATGCACCCTTGCAGAAAGAATTATCTGCTGAGGAAGTGGGGAATGCTGCTGCCTTCCTGTCATCACCATTGGCATCAGCTATCACCGGTACTGTTCTATACGTCGACAATGGTCTAAATGCAATGGGAGTGGGAGTTGACAGCCCAGTTTTTGAAAACCTTGATATTCCAAAAGCAGCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

393

Amino Acids

41.53

Weight (kDa)

8.5

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 3 cut(s) 439, 494, 867
AciI CCGC 2 cut(s) 494, 944
AclI AACGTT 1 cut(s) 900
AclWI GGATC 3 cut(s) 18, 31, 812
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 91, 363
AcuI CTGAAG 2 cut(s) 63, 543
AfaI GTAC 4 cut(s) 100, 376, 788, 1084
AfeI AGCGCT 1 cut(s) 545
AfiI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 818
AflIII ACRYGT 1 cut(s) 865
AgeI ACCGGT 1 cut(s) 1079
AgsI TTSAA 4 cut(s) 181, 587, 774, 1148
AjnI CCWGG 3 cut(s) 19, 253, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 4 cut(s) 177, 236, 884, 1073
AluI AGCT 4 cut(s) 177, 236, 884, 1073
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 3 cut(s) 18, 31, 812
Aor51HI AGCGCT 1 cut(s) 545
AoxI GGCC 3 cut(s) 3, 188, 873
ApoI RAATTY 2 cut(s) 91, 363
AsiGI ACCGGT 1 cut(s) 1079
AspLEI GCGC 2 cut(s) 441, 546
AspS9I GGNCC 4 cut(s) 188, 555, 644, 926
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 3 cut(s) 205, 1050, 1069
AsuII TTCGAA 2 cut(s) 402, 879
AvaII GGWCC 3 cut(s) 555, 644, 926
AxyI CCTNAGG 1 cut(s) 510
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 58, 90
BbvCI CCTCAGC 1 cut(s) 1023
BccI CCATC 2 cut(s) 318, 446
BciT130I CCWGG 3 cut(s) 21, 255, 762
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 2 cut(s) 368, 851
BfoI RGCGCY 1 cut(s) 547
Bme1390I CCNGG 4 cut(s) 21, 255, 762, 813
Bme18I GGWCC 3 cut(s) 555, 644, 926
BmgT120I GGNCC 4 cut(s) 188, 555, 644, 926
BmiI GGNNCC 3 cut(s) 25, 384, 556
BmrFI CCNGG 4 cut(s) 21, 255, 762, 813
BmrI ACTGGG 1 cut(s) 1133
BmsI GCATC 2 cut(s) 716, 1076
BmuI ACTGGG 1 cut(s) 1133
BoxI GACNNNNGTC 1 cut(s) 1104
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu14I TTCGAA 2 cut(s) 402, 879
BpuEI CTTGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 813
BsaJI CCNNGG 3 cut(s) 474, 760, 812
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1113, 1143
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 414, 564, 818
Bse118I RCCGGY 2 cut(s) 385, 1079
Bse1I ACTGG 2 cut(s) 408, 1139
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 4 cut(s) 228, 291, 789, 1122
BseBI CCWGG 3 cut(s) 21, 255, 762
BseDI CCNNGG 3 cut(s) 474, 760, 812
BseGI GGATG 2 cut(s) 329, 520
BseLI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 818
BseMI GCAATG 4 cut(s) 228, 291, 789, 1122
BseMII CTCAG 4 cut(s) 165, 228, 501, 1014
BseNI ACTGG 2 cut(s) 408, 1139
BseRI GAGGAG 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 353
Bsh1236I CGCG 3 cut(s) 439, 494, 867
BshFI GGCC 3 cut(s) 5, 190, 875
BshNI GGYRCC 1 cut(s) 382
BshTI ACCGGT 1 cut(s) 1079
BsiSI CCGG 3 cut(s) 386, 812, 1080
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 4 cut(s) 254, 414, 564, 818
BsmAI GTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 2 cut(s) 287, 1042
BsnI GGCC 3 cut(s) 5, 190, 875
Bsp119I TTCGAA 2 cut(s) 402, 879
Bsp143I GATC 3 cut(s) 23, 310, 804
BspACI CCGC 2 cut(s) 494, 944
BspANI GGCC 3 cut(s) 5, 190, 875
BspCNI CTCAG 4 cut(s) 164, 229, 502, 1015
BspFNI CGCG 3 cut(s) 439, 494, 867
BspLI GGNNCC 3 cut(s) 25, 384, 556
BspPI GGATC 3 cut(s) 18, 31, 812
BspT104I TTCGAA 2 cut(s) 402, 879
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 4 cut(s) 228, 291, 789, 1122
BsrFI RCCGGY 2 cut(s) 385, 1079
BsrI ACTGG 2 cut(s) 408, 1139
BssAI RCCGGY 2 cut(s) 385, 1079
BssECI CCNNGG 3 cut(s) 474, 760, 812
BssMI GATC 3 cut(s) 23, 310, 804
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 21, 255, 762
Bst4CI ACNGT 4 cut(s) 107, 245, 571, 1087
BstAPI GCANNNNNTGC 2 cut(s) 293, 704
BstBI TTCGAA 2 cut(s) 402, 879
BstC8I GCNNGC 3 cut(s) 387, 869, 873
BstDEI CTNAG 4 cut(s) 151, 237, 510, 1023
BstF5I GGATG 2 cut(s) 329, 520
BstFNI CGCG 3 cut(s) 439, 494, 867
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 2 cut(s) 441, 546
BstKTI GATC 3 cut(s) 26, 313, 807
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 3 cut(s) 23, 310, 804
BstMWI GCNNNNNNNGC 9 cut(s) 259, 293, 302, 356, 445, 704, 845, 881, 950
BstNI CCWGG 3 cut(s) 21, 255, 762
BstPAI GACNNNNGTC 1 cut(s) 1104
BstSCI CCNGG 4 cut(s) 19, 253, 760, 811
BstUI CGCG 3 cut(s) 439, 494, 867
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 510
BsuRI GGCC 3 cut(s) 5, 190, 875
BtsCI GGATG 2 cut(s) 329, 520
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 3 cut(s) 387, 869, 873
CfoI GCGC 2 cut(s) 441, 546
Cfr10I RCCGGY 2 cut(s) 385, 1079
Cfr13I GGNCC 4 cut(s) 188, 555, 644, 926
CseI GACGC 2 cut(s) 500, 783
Csp6I GTAC 4 cut(s) 99, 375, 787, 1083
CspAI ACCGGT 1 cut(s) 1079
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CviAII CATG 3 cut(s) 31, 314, 829
CviQI GTAC 4 cut(s) 99, 375, 787, 1083
DdeI CTNAG 4 cut(s) 151, 237, 510, 1023
DpnI GATC 3 cut(s) 25, 312, 806
DpnII GATC 3 cut(s) 23, 310, 804
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 474
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 3 cut(s) 555, 644, 926
Eco47III AGCGCT 1 cut(s) 545
Eco57I CTGAAG 2 cut(s) 63, 543
Eco81I CCTNAGG 1 cut(s) 510
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoRII CCWGG 3 cut(s) 19, 253, 760
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 3 cut(s) 34, 317, 832
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 503
FatI CATG 3 cut(s) 30, 313, 828
FauNDI CATATG 1 cut(s) 721
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 1098
FokI GGATG 2 cut(s) 336, 527
FspBI CTAG 2 cut(s) 368, 851
GlaI GCGC 2 cut(s) 440, 545
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 3 cut(s) 5, 190, 875
HapII CCGG 3 cut(s) 386, 812, 1080
HgaI GACGC 2 cut(s) 500, 783
HhaI GCGC 2 cut(s) 441, 546
Hin1II CATG 3 cut(s) 34, 317, 832
Hin6I GCGC 2 cut(s) 439, 544
HinP1I GCGC 2 cut(s) 439, 544
HincII GTYRAC 3 cut(s) 910, 1099, 1132
HindII GTYRAC 3 cut(s) 910, 1099, 1132
HinfI GANTC 4 cut(s) 404, 434, 587, 906
HpaII CCGG 3 cut(s) 386, 812, 1080
HphI GGTGA 3 cut(s) 205, 1050, 1069
Hpy166II GTNNAC 5 cut(s) 481, 628, 910, 1099, 1132
Hpy188I TCNGA 2 cut(s) 799, 991
Hpy188III TCNNGA 6 cut(s) 63, 172, 228, 509, 678, 851
Hpy8I GTNNAC 5 cut(s) 481, 628, 910, 1099, 1132
Hpy99I CGWCG 1 cut(s) 1100
HpyAV CCTTC 6 cut(s) 57, 120, 568, 835, 886, 1057
HpyCH4III ACNGT 4 cut(s) 107, 245, 571, 1087
HpyCH4IV ACGT 3 cut(s) 785, 900, 1095
HpyCH4V TGCA 5 cut(s) 695, 707, 998, 1006, 1115
HpyF10VI GCNNNNNNNGC 9 cut(s) 259, 293, 302, 356, 445, 704, 845, 881, 950
HpyF3I CTNAG 4 cut(s) 151, 237, 510, 1023
HpySE526I ACGT 3 cut(s) 785, 900, 1095
Hsp92II CATG 3 cut(s) 34, 317, 832
HspAI GCGC 2 cut(s) 439, 544
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 23, 310, 804
LmnI GCTCC 2 cut(s) 230, 751
LweI GCATC 2 cut(s) 716, 1076
MaeI CTAG 2 cut(s) 368, 851
MaeII ACGT 3 cut(s) 785, 900, 1095
MaeIII GTNAC 4 cut(s) 211, 652, 713, 857
MalI GATC 3 cut(s) 25, 312, 806
MboI GATC 3 cut(s) 23, 310, 804
MboII GAAGA 5 cut(s) 78, 132, 160, 536, 900
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 1 cut(s) 23
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 8 cut(s) 91, 363, 443, 468, 562, 753, 954, 1013
MluI ACGCGT 1 cut(s) 865
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 443, 915
MmeI TCCRAC 1 cut(s) 561
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 42, 132, 393, 413, 461, 756, 932
MslI CAYNNNNRTG 3 cut(s) 318, 866, 968
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 386, 812, 1080
MspR9I CCNGG 4 cut(s) 21, 255, 762, 813
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 2 cut(s) 287, 1042
MvaI CCWGG 3 cut(s) 21, 255, 762
MvnI CGCG 3 cut(s) 439, 494, 867
MwoI GCNNNNNNNGC 9 cut(s) 259, 293, 302, 356, 445, 704, 845, 881, 950
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 813
NdeI CATATG 1 cut(s) 721
NdeII GATC 3 cut(s) 23, 310, 804
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 3 cut(s) 34, 317, 832
NlaIV GGNNCC 3 cut(s) 25, 384, 556
NmuCI GTSAC 2 cut(s) 211, 857
NspV TTCGAA 2 cut(s) 402, 879
OliI CACNNNNGTG 1 cut(s) 866
PcsI WCGNNNNNNNCGW 1 cut(s) 791
PctI GAATGC 2 cut(s) 287, 1042
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 404, 587
PfoI TCCNGGA 1 cut(s) 19
PinAI ACCGGT 1 cut(s) 1079
PleI GAGTC 2 cut(s) 442, 914
PpsI GAGTC 2 cut(s) 442, 914
PpuMI RGGWCCY 1 cut(s) 555
PshAI GACNNNNGTC 1 cut(s) 1104
Psp1406I AACGTT 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 3 cut(s) 19, 253, 760
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 3 cut(s) 19, 253, 760
PspN4I GGNNCC 3 cut(s) 25, 384, 556
PspPI GGNCC 4 cut(s) 188, 555, 644, 926
PspPPI RGGWCCY 1 cut(s) 555
PsuI RGATCY 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 4 cut(s) 100, 376, 788, 1084
RsaNI GTAC 4 cut(s) 99, 375, 787, 1083
RseI CAYNNNNRTG 3 cut(s) 318, 866, 968
SalI GTCGAC 1 cut(s) 1097
SaqAI TTAA 7 cut(s) 42, 132, 393, 413, 461, 756, 932
Sau3AI GATC 3 cut(s) 23, 310, 804
Sau96I GGNCC 4 cut(s) 188, 555, 644, 926
SchI GAGTC 2 cut(s) 443, 915
ScrFI CCNGG 4 cut(s) 21, 255, 762, 813
SfaNI GCATC 2 cut(s) 716, 1076
SfuI TTCGAA 2 cut(s) 402, 879
SinI GGWCC 3 cut(s) 555, 644, 926
SmiMI CAYNNNNRTG 3 cut(s) 318, 866, 968
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 8 cut(s) 91, 363, 443, 468, 562, 753, 954, 1013
SsiI CCGC 2 cut(s) 494, 944
SspMI CTAG 2 cut(s) 368, 851
StyD4I CCNGG 4 cut(s) 19, 253, 760, 811
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 4 cut(s) 107, 245, 571, 1087
TaiI ACGT 3 cut(s) 788, 903, 1098
TaqI TCGA 5 cut(s) 64, 402, 710, 879, 1098
TasI AATT 8 cut(s) 91, 363, 443, 468, 562, 753, 954, 1013
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 2 cut(s) 404, 587
Tru1I TTAA 7 cut(s) 42, 132, 393, 413, 461, 756, 932
Tru9I TTAA 7 cut(s) 42, 132, 393, 413, 461, 756, 932
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 2 cut(s) 211, 857
Tsp45I GTSAC 2 cut(s) 211, 857
TspDTI ATGAA 2 cut(s) 280, 952
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 3 cut(s) 555, 644, 926
XapI RAATTY 2 cut(s) 91, 363
XbaI TCTAGA 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 2 cut(s) 368, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.