Rmu_sc0007249.1_g000002

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007249.1
OG0001646
Physical Location & Seq
Forward (+)
9476 .. 14163
4688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007249.1_g000002.1.cds

Sequence Viewer

Length: 1422 bp
atgggaatggggcatattagagctgaacccttccaatcaagtccaaaggtccagttaagcacaagggcaaacccgtcattacacaggggcgccaaaagcaacgaacagatcaacgctctgtttcggcatttacaaagtctgccaactccaactgaccaaccctccaacaaaccaaaagcctcaggctttggtttcatcgaacccccacacaaaaccacactccctcacatttatataaaaacccctcactgcaacccttccttctttgctctctcaaacactatcagatttcgaagagttttgttttggtttctggtgatctgtatcgaaaatggccactactgcagctcctgtgaagtggcagtttctaaggggcttagaagctcttcccacatctcatcaaggcaaccattcttcccgagcatccaatcaggccctgcgaagtctgtaaaggttatcacaagagcaatgtcagcagcagctgagaactctccattgcctggattgcctattgatttgagaggaaagagagcattcattgctggtgtggctgatgataatggatatggatgggcaatagcaaaacaacttgctgctgctggtgctgaaattcttcttggtacatgggtgccggcacttaacattttcgagtccagtttaaggcgtgggaagtttgatgaatcacgcatattgcctgacggttctttgatggaaattacaaaagtctatccattggatgcagtttttgacaatcctgatgatgttcctgaagatataaaatcaaacaaacgctacttggggtcctcaaagtggacggttaaggaggttgctgaatctgttaaggaggattttggcagcattgatatcctagtccattccctggctaatggaccagaggttgtcaaacctcttatggaaacatcaagatatggatatcttgcagctatctctgcatcaagctactcgtttatttctttactccagcattttgctccaataatgaacccaggtggtgcgtcaatctctctgacatacattgcttccgagaggatcattcccggatatggtggaggtatgagttcagcgaaggctgctctagagagtgacacacgtgtgctggcctttgaagctgggagaaaacacagactcagagtcaacacaatatctgcaggcccattaagaagtcgtgctgcaaaagctattggttttatcgatatgatgattgattactcatcagccaatgctcccttaccgaaagagttgtctgctgaggaggtggggaacgttgctgccttccttgcatcaccattggcatcagcaatcaccggcagtgttgtatacgtcgacaatggtctaaatgcaatgggtgtgggagttgacagcccaatttttgaaaaccttgagcttccaaaagaagcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

473

Amino Acids

50.97

Weight (kDa)

8.85

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 89, 628
AccB7I CCANNNNNTGG 2 cut(s) 500, 880
AccI GTMKAC 2 cut(s) 1338, 1344
AclI AACGTT 1 cut(s) 1284
AclWI GGATC 1 cut(s) 1058
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 1 cut(s) 609
AcuI CTGAAG 1 cut(s) 789
AcvI CACGTG 1 cut(s) 1112
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 622
AfiI CCNNNNNNNGG 5 cut(s) 500, 660, 810, 880, 1064
AflIII ACRYGT 2 cut(s) 1109, 1111
AgsI TTSAA 2 cut(s) 1127, 1394
AhdI GACNNNNNGTC 1 cut(s) 1151
AjnI CCWGG 3 cut(s) 499, 879, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AleI CACNNNNGTG 1 cut(s) 1112
AluBI AGCT 9 cut(s) 23, 348, 384, 482, 944, 960, 1130, 1199, 1405
AluI AGCT 9 cut(s) 23, 348, 384, 482, 944, 960, 1130, 1199, 1405
AlwI GGATC 1 cut(s) 1058
AlwNI CAGNNNCTG 3 cut(s) 351, 437, 482
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 4 cut(s) 334, 433, 1119, 1171
ApoI RAATTY 1 cut(s) 609
ArsI GACNNNNNNTTYG 2 cut(s) 1247, 1279
Asp700I GAANNNNTTC 2 cut(s) 385, 612
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 5 cut(s) 49, 434, 801, 890, 1172
AsuC2I CCSGG 1 cut(s) 1059
AsuHPI GGTGA 3 cut(s) 328, 1296, 1315
AsuII TTCGAA 1 cut(s) 292
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 3 cut(s) 49, 801, 890
AxyI CCTNAGG 1 cut(s) 181
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 2 cut(s) 89, 628
BbrPI CACGTG 1 cut(s) 1112
BbvCI CCTCAGC 1 cut(s) 1269
BccI CCATC 2 cut(s) 564, 703
BciT130I CCWGG 3 cut(s) 501, 881, 1008
BcnI CCSGG 1 cut(s) 1059
BfaI CTAG 3 cut(s) 869, 1097, 1420
BfmI CTRYAG 2 cut(s) 343, 1167
BfoI RGCGCY 1 cut(s) 93
Bme1390I CCNGG 4 cut(s) 501, 881, 1008, 1059
Bme18I GGWCC 3 cut(s) 49, 801, 890
BmeRI GACNNNNNGTC 1 cut(s) 1151
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 5 cut(s) 49, 434, 801, 890, 1172
BmiI GGNNCC 3 cut(s) 91, 630, 802
BmrFI CCNGG 4 cut(s) 501, 881, 1008, 1059
BmsI GCATC 5 cut(s) 432, 727, 962, 1310, 1322
BoxI GACNNNNGTC 1 cut(s) 1350
BpmI CTGGAG 1 cut(s) 965
Bpu10I CCTNAGC 1 cut(s) 1269
Bpu14I TTCGAA 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 1421
BpuMI CCSGG 1 cut(s) 1059
Bsa29I ATCGAT 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 1112
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 879, 1006
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 332, 362
Bsc4I CCNNNNNNNGG 5 cut(s) 500, 660, 810, 880, 1064
Bse118I RCCGGY 2 cut(s) 631, 1325
Bse1I ACTGG 2 cut(s) 52, 654
Bse21I CCTNAGG 1 cut(s) 181
Bse3DI GCAATG 5 cut(s) 474, 494, 537, 1035, 1368
Bse8I GATNNNNATC 1 cut(s) 323
BseBI CCWGG 3 cut(s) 501, 881, 1008
BseCI ATCGAT 1 cut(s) 1212
BseDI CCNNGG 2 cut(s) 879, 1006
BseGI GGATG 3 cut(s) 423, 575, 742
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 5 cut(s) 500, 660, 810, 880, 1064
BseMI GCAATG 5 cut(s) 474, 494, 537, 1035, 1368
BseMII CTCAG 4 cut(s) 195, 474, 1162, 1260
BseNI ACTGG 2 cut(s) 52, 654
BseRI GAGGAG 1 cut(s) 1286
BseYI CCCAGC 1 cut(s) 1130
BshFI GGCC 4 cut(s) 336, 435, 1121, 1173
BshNI GGYRCC 2 cut(s) 89, 628
BshVI ATCGAT 1 cut(s) 1212
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 3 cut(s) 632, 1059, 1326
BslI CCNNNNNNNGG 5 cut(s) 500, 660, 810, 880, 1064
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 4 cut(s) 336, 435, 1121, 1173
BsoBI CYCGRG 1 cut(s) 418
Bsp119I TTCGAA 1 cut(s) 292
Bsp143I GATC 3 cut(s) 108, 318, 1050
BspANI GGCC 4 cut(s) 336, 435, 1121, 1173
BspCNI CTCAG 4 cut(s) 194, 475, 1161, 1261
BspDI ATCGAT 1 cut(s) 1212
BspLI GGNNCC 3 cut(s) 91, 630, 802
BspMAI CTGCAG 2 cut(s) 347, 1171
BspPI GGATC 1 cut(s) 1058
BspQI GCTCTTC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 292
BspT107I GGYRCC 2 cut(s) 89, 628
BsrDI GCAATG 5 cut(s) 474, 494, 537, 1035, 1368
BsrFI RCCGGY 2 cut(s) 631, 1325
BsrI ACTGG 2 cut(s) 52, 654
BssAI RCCGGY 2 cut(s) 631, 1325
BssECI CCNNGG 2 cut(s) 879, 1006
BssMI GATC 3 cut(s) 108, 318, 1050
BssNAI GTATAC 1 cut(s) 1339
BssNI GRCGYC 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 1339
Bst2UI CCWGG 3 cut(s) 501, 881, 1008
Bst4CI ACNGT 2 cut(s) 701, 817
Bst6I CTCTTC 2 cut(s) 289, 391
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 539
BstBAI YACGTR 1 cut(s) 1112
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 3 cut(s) 633, 1119, 1171
BstDEI CTNAG 6 cut(s) 181, 369, 377, 483, 1148, 1269
BstF5I GGATG 3 cut(s) 423, 575, 742
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 3 cut(s) 111, 321, 1053
BstMBI GATC 3 cut(s) 108, 318, 1050
BstNI CCWGG 3 cut(s) 501, 881, 1008
BstPAI GACNNNNGTC 1 cut(s) 1350
BstSCI CCNGG 4 cut(s) 499, 879, 1006, 1057
BstSFI CTRYAG 2 cut(s) 343, 1167
BstZ17I GTATAC 1 cut(s) 1339
Bsu15I ATCGAT 1 cut(s) 1212
Bsu36I CCTNAGG 1 cut(s) 181
BsuRI GGCC 4 cut(s) 336, 435, 1121, 1173
BsuTUI ATCGAT 1 cut(s) 1212
BtsCI GGATG 3 cut(s) 423, 575, 742
BtsI GCAGTG 2 cut(s) 247, 1336
BtsIMutI CAGTG 2 cut(s) 247, 1336
Cac8I GCNNGC 3 cut(s) 633, 1119, 1171
CaiI CAGNNNCTG 3 cut(s) 351, 437, 482
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 2 cut(s) 631, 1325
Cfr13I GGNCC 5 cut(s) 49, 434, 801, 890, 1172
ClaI ATCGAT 1 cut(s) 1212
CseI GACGC 1 cut(s) 1005
Csp6I GTAC 1 cut(s) 621
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviAII CATG 1 cut(s) 624
CviQI GTAC 1 cut(s) 621
DdeI CTNAG 6 cut(s) 181, 369, 377, 483, 1148, 1269
DinI GGCGCC 1 cut(s) 91
DpnI GATC 3 cut(s) 110, 320, 1052
DpnII GATC 3 cut(s) 108, 318, 1050
DriI GACNNNNNGTC 1 cut(s) 1151
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 289, 391
Eam1105I GACNNNNNGTC 1 cut(s) 1151
EarI CTCTTC 2 cut(s) 289, 391
Eco32I GATATC 2 cut(s) 865, 935
Eco47I GGWCC 3 cut(s) 49, 801, 890
Eco57I CTGAAG 1 cut(s) 789
Eco72I CACGTG 1 cut(s) 1112
Eco81I CCTNAGG 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 418
EcoO109I RGGNCCY 2 cut(s) 434, 801
EcoRII CCWGG 3 cut(s) 499, 879, 1006
EcoRV GATATC 2 cut(s) 865, 935
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
FaeI CATG 1 cut(s) 627
FatI CATG 1 cut(s) 623
FblI GTMKAC 2 cut(s) 1338, 1344
FokI GGATG 3 cut(s) 410, 582, 749
FspBI CTAG 3 cut(s) 869, 1097, 1420
GlaI GCGC 1 cut(s) 91
GsaI CCCAGC 1 cut(s) 1134
GsuI CTGGAG 1 cut(s) 965
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 4 cut(s) 336, 435, 1121, 1173
HapII CCGG 3 cut(s) 632, 1059, 1326
HgaI GACGC 1 cut(s) 1005
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 1 cut(s) 627
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 3 cut(s) 1156, 1345, 1378
HindII GTYRAC 3 cut(s) 1156, 1345, 1378
HinfI GANTC 5 cut(s) 650, 680, 833, 1146, 1152
HpaII CCGG 3 cut(s) 632, 1059, 1326
HphI GGTGA 3 cut(s) 328, 1296, 1315
Hpy166II GTNNAC 5 cut(s) 813, 1156, 1339, 1345, 1378
Hpy188I TCNGA 4 cut(s) 287, 1029, 1045, 1151
Hpy188III TCNNGA 5 cut(s) 418, 755, 767, 924, 1097
Hpy8I GTNNAC 5 cut(s) 813, 1156, 1339, 1345, 1378
Hpy99I CGWCG 1 cut(s) 1346
HpyAV CCTTC 5 cut(s) 40, 267, 271, 1081, 1303
HpyCH4III ACNGT 2 cut(s) 701, 817
HpyCH4IV ACGT 3 cut(s) 1111, 1284, 1341
HpyCH4V TGCA 9 cut(s) 252, 345, 740, 941, 953, 1169, 1193, 1301, 1361
HpyF3I CTNAG 6 cut(s) 181, 369, 377, 483, 1148, 1269
HpySE526I ACGT 3 cut(s) 1111, 1284, 1341
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 1 cut(s) 627
HspAI GCGC 1 cut(s) 90
KasI GGCGCC 1 cut(s) 89
KroI GCCGGC 1 cut(s) 631
KroNI GCCGGC 1 cut(s) 633
Kzo9I GATC 3 cut(s) 108, 318, 1050
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 3 cut(s) 353, 997, 1249
LweI GCATC 5 cut(s) 432, 727, 962, 1310, 1322
MaeI CTAG 3 cut(s) 869, 1097, 1420
MaeII ACGT 3 cut(s) 1111, 1284, 1341
MaeIII GTNAC 1 cut(s) 1103
MalI GATC 3 cut(s) 110, 320, 1052
MboI GATC 3 cut(s) 108, 318, 1050
MboII GAAGA 5 cut(s) 306, 378, 406, 605, 782
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 3 cut(s) 609, 714, 1386
MluNI TGGCCA 1 cut(s) 336
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 3 cut(s) 659, 1140, 1161
MmeI TCCRAC 2 cut(s) 173, 189
Mox20I TGGCCA 1 cut(s) 336
MroNI GCCGGC 1 cut(s) 631
MroXI GAANNNNTTC 2 cut(s) 385, 612
MscI TGGCCA 1 cut(s) 336
MseI TTAA 6 cut(s) 56, 639, 659, 819, 840, 1178
MslI CAYNNNNRTG 1 cut(s) 1112
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 3 cut(s) 632, 1059, 1326
MspR9I CCNGG 4 cut(s) 501, 881, 1008, 1059
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 3 cut(s) 501, 881, 1008
NaeI GCCGGC 1 cut(s) 633
NarI GGCGCC 1 cut(s) 90
NciI CCSGG 1 cut(s) 1059
NdeII GATC 3 cut(s) 108, 318, 1050
NgoMIV GCCGGC 1 cut(s) 631
NlaIII CATG 1 cut(s) 627
NlaIV GGNNCC 3 cut(s) 91, 630, 802
NmuCI GTSAC 1 cut(s) 1103
NspV TTCGAA 1 cut(s) 292
OliI CACNNNNGTG 1 cut(s) 1112
PciSI GCTCTTC 1 cut(s) 391
PctI GAATGC 1 cut(s) 533
PdiI GCCGGC 1 cut(s) 633
PdmI GAANNNNTTC 2 cut(s) 385, 612
PfeI GAWTC 2 cut(s) 680, 833
PflMI CCANNNNNTGG 2 cut(s) 500, 880
PfoI TCCNGGA 1 cut(s) 1057
PleI GAGTC 3 cut(s) 658, 1140, 1160
PluTI GGCGCC 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 1112
PmlI CACGTG 1 cut(s) 1112
PpsI GAGTC 3 cut(s) 658, 1140, 1160
Ppu21I YACGTR 1 cut(s) 1112
PpuMI RGGWCCY 1 cut(s) 801
PshAI GACNNNNGTC 1 cut(s) 1350
Psp1406I AACGTT 1 cut(s) 1284
Psp5II RGGWCCY 1 cut(s) 801
Psp6I CCWGG 3 cut(s) 499, 879, 1006
PspCI CACGTG 1 cut(s) 1112
PspFI CCCAGC 1 cut(s) 1130
PspGI CCWGG 3 cut(s) 499, 879, 1006
PspN4I GGNNCC 3 cut(s) 91, 630, 802
PspPI GGNCC 5 cut(s) 49, 434, 801, 890, 1172
PspPPI RGGWCCY 1 cut(s) 801
PstI CTGCAG 2 cut(s) 347, 1171
PstNI CAGNNNCTG 3 cut(s) 351, 437, 482
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 622
RsaNI GTAC 1 cut(s) 621
RseI CAYNNNNRTG 1 cut(s) 1112
SalI GTCGAC 1 cut(s) 1343
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 6 cut(s) 56, 639, 659, 819, 840, 1178
Sau3AI GATC 3 cut(s) 108, 318, 1050
Sau96I GGNCC 5 cut(s) 49, 434, 801, 890, 1172
SchI GAGTC 3 cut(s) 659, 1140, 1161
ScrFI CCNGG 4 cut(s) 501, 881, 1008, 1059
SfaNI GCATC 5 cut(s) 432, 727, 962, 1310, 1322
SfcI CTRYAG 2 cut(s) 343, 1167
SfoI GGCGCC 1 cut(s) 91
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 3 cut(s) 49, 801, 890
SmiMI CAYNNNNRTG 1 cut(s) 1112
SmlI CTYRAG 1 cut(s) 1400
SmoI CTYRAG 1 cut(s) 1400
Sse9I AATT 3 cut(s) 609, 714, 1386
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 3 cut(s) 869, 1097, 1420
StyD4I CCNGG 4 cut(s) 499, 879, 1006, 1057
TaaI ACNGT 2 cut(s) 701, 817
TaiI ACGT 3 cut(s) 1114, 1287, 1344
TaqI TCGA 6 cut(s) 198, 292, 327, 648, 1212, 1344
TasI AATT 3 cut(s) 609, 714, 1386
TfiI GAWTC 2 cut(s) 680, 833
Tru1I TTAA 6 cut(s) 56, 639, 659, 819, 840, 1178
Tru9I TTAA 6 cut(s) 56, 639, 659, 819, 840, 1178
TscAI CASTG 2 cut(s) 254, 1336
TseFI GTSAC 1 cut(s) 1103
Tsp45I GTSAC 1 cut(s) 1103
TspDTI ATGAA 4 cut(s) 184, 526, 693, 1016
TspRI CASTG 2 cut(s) 254, 1336
Van91I CCANNNNNTGG 2 cut(s) 500, 880
VpaK11BI GGWCC 3 cut(s) 49, 801, 890
XapI RAATTY 1 cut(s) 609
XbaI TCTAGA 1 cut(s) 1096
XmiI GTMKAC 2 cut(s) 1338, 1344
XmnI GAANNNNTTC 2 cut(s) 385, 612
XspI CTAG 3 cut(s) 869, 1097, 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.