Rorug03G0179000

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000940
Physical Location & Seq
Forward (+)
15215636 .. 15220798
5163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0179000.1

Sequence Viewer

Length: 1809 bp
ATGGAGGCCCTTCACCTTAGCTCAGTTCAGTCCCCTATTCTCCCACCAAAACGGTTTGAATCATTCCCTCTCGGGACCCAAAAGGTTAGCACACGCCGTCCCAACAATCCATTGTTCATCACTGCCACATTGTCCCGACCAACAACTGAGGTTTCCGCTGAACCCTCCTCCTCCTCTGCATCTATCCTGACTCCGTCATCGCCTCCCAATCGGGTCTCTACTAGTTCCTTACAGTATGAACAAGGTTTTCTTGGGGCAGTACCTGACCGTACGGTTGCTAGTGGAAACAACGACATCGTTGACGCTCTGGGATCTTTGACGAACATATTGACGTCAAAAGTGTATGACCTGGCAATTGAATCGCCTTTAGAGCTGGCAACCAAGCTATCCGAGCGGTTGGGAGTGAACATTTGGCTCAAAAGGGAAGATTTGCAGCCGGTGTTTTCGTTCAAGCTTCGTGGAGCTTATAATATGATGGCTAAGATTCCAAGGGAACAATTGGACAGAGGGGTTATATGCTCGTCGGCTGGAAACCATGCGCAAGGAGTTGCATTGTCCGCCAAGAAATTGGGTTGCAATGCAGTCATTGCAATGCCTGTAACTACTCCAGAAATTAAGTGGCAATCTGTTGAGAGATTGGGAGCCACGGTTGTACTTATAGGTGATTCCTACGATGAGGCTCAAGCTTATGCTAAGAAGCGAGCTAAGGAGGAGGGACGGAGCTTCATACCTCCTTTTGATCACCCAGACATTATTATGGGTCAGGGAACAGTCGGAATGGAGGTTATGCGTCAAACAAAAGAGCCATTGCATGCAATTTTTGTGCCGGTGGGGGGTGGTGGCCTAATAGCTGGTATTGCTGCATACGTTAAGAGGCTGTCTCCAGAGGTAAAGATTATTGGTGTGGAGCCCTCGGATGCGAATGCAATGGCATTATCCTTGCATCACGGAGAGAGAATAATGTTGGACCAAGTTGGAGGTTTTGCAGATGGTGTAGCAGTGAAAGAGGTGGGTGAAGAAACCTTTCGGATATGCAAAGAATTGATAGATGGTGTAGTTCTCGTGAGCCGTGACGCCATCTGTGGTTCAATAAAGGACATGTTTGAGGAGACTAGAAGCATTTTAGAACCAGCAGGTGCCCTTGCTCTTGCTGGAGCTGAAGCATACTGCAAGTATTATGGTCTTAAAGGAGAAAATGTTGTTGCAATTACCAGCGGGGCAAATATGAACTTTGATAAACTGAGAGTGGTGACTGAACTTGCAAATGTTGGTCGGCAACAAGAGGCTGTACTCGCAACAGTTATGCCAGAGGTGCCTGGGAGCTTTAAACATTACTGTGAGCTGGTCGGCCCAATGAATATTAGTGAATTCAAATACAGATATAACTCTGATAAAAAGGCTGTCGTACTGTACAGTGTTGGTGTGCACACAGCTTCTGAACTTGAAGCAATGCAAGAGCGCATGGAGTCTTCTCAACTCAAAACGATCAATCTCACGAAAAGTGACTTGGTTAAAGATCACTTGCGTTACTTGATGGGGGGCAGAACAAATGTTGAAAATGAGGTTCTTTGTCGCTTTGTCTTCCCAGAAAGGCCTGGTGCTTTGGTGAAGTTCTTGGACACCTTTAGTCCACGTTGGAATATTAGTTTGTTCCACTACCGCGGACAGGGTGAAACTGGGGCAAATGTCTTGGTTGGAATCCAAGTTTCCAAGAGTGAGATTGATGAGTTCCATTCTCGTGCCAATTCACTTGGGTATGACTATGTAGTAGTGACCAATGATATAGACTTCGAGCTTTTGATGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

602

Amino Acids

65.72

Weight (kDa)

5.93

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 120 - 406 1.3e-79 Pyridoxal-phosphate dependent enzyme
Thr_dehydrat_C PF00585 419 - 508 1.3e-21 C-terminal regulatory domain of Threonine dehydratase
Thr_dehydrat_C PF00585 514 - 597 5.6e-23 C-terminal regulatory domain of Threonine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AarI CACCTGC 1 cut(s) 1124
AatII GACGTC 1 cut(s) 335
Acc16I TGCGCA 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 1124
AccB1I GGYRCC 2 cut(s) 1136, 1312
AccBSI CCGCTC 1 cut(s) 394
AccII CGCG 1 cut(s) 1662
AciI CCGC 6 cut(s) 156, 394, 558, 1215, 1660, 1662
AclWI GGATC 1 cut(s) 319
AcsI RAATTY 1 cut(s) 1367
AcuI CTGAAG 1 cut(s) 1179
AcyI GRCGYC 2 cut(s) 332, 1074
AfaI GTAC 6 cut(s) 261, 271, 654, 1290, 1407, 1412
AfiI CCNNNNNNNGG 2 cut(s) 833, 1666
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 7 cut(s) 59, 359, 451, 1089, 1372, 1445, 1556
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 3 cut(s) 348, 1315, 1594
AjuI GAANNNNNNNTTGG 2 cut(s) 1490, 1522
Alw21I GWGCWC 1 cut(s) 1428
Alw26I GTCTC 3 cut(s) 220, 885, 1103
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 1 cut(s) 319
AlwNI CAGNNNCTG 2 cut(s) 263, 1436
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 4 cut(s) 6, 841, 1348, 1592
ApaLI GTGCAC 1 cut(s) 1424
ApeKI GCWGC 2 cut(s) 433, 860
ApoI RAATTY 1 cut(s) 1367
Asp700I GAANNNNTTC 1 cut(s) 1023
AspLEI GCGC 2 cut(s) 541, 1461
AspS9I GGNCC 4 cut(s) 7, 75, 967, 1349
AsuHPI GGTGA 7 cut(s) 5, 674, 734, 1025, 1261, 1618, 1682
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 2 cut(s) 75, 967
BaeGI GKGCMC 2 cut(s) 1141, 1428
BanI GGYRCC 2 cut(s) 1136, 1312
BanII GRGCYC 1 cut(s) 912
BauI CACGAG 2 cut(s) 1061, 1737
BbsI GAAGAC 2 cut(s) 1461, 1573
Bbv12I GWGCWC 1 cut(s) 1428
BbvI GCAGC 2 cut(s) 445, 847
BccI CCATC 5 cut(s) 469, 983, 1043, 1085, 1528
BceAI ACGGC 2 cut(s) 81, 1053
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 3 cut(s) 350, 1317, 1596
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 220, 885, 1103
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 3 cut(s) 222, 279, 1113
BfuAI ACCTGC 1 cut(s) 1124
BisI GCNGC 2 cut(s) 434, 861
BlsI GCNGC 2 cut(s) 435, 862
Bme1390I CCNGG 3 cut(s) 350, 1317, 1596
Bme18I GGWCC 2 cut(s) 75, 967
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 4 cut(s) 7, 75, 967, 1349
BmiI GGNNCC 6 cut(s) 76, 77, 643, 909, 1138, 1314
BmrFI CCNGG 3 cut(s) 350, 1317, 1596
BmrI ACTGGG 1 cut(s) 1686
BmsI GCATC 4 cut(s) 188, 907, 952, 1791
BmuI ACTGGG 1 cut(s) 1686
BpiI GAAGAC 2 cut(s) 1461, 1573
BplI GAGNNNNNCTC 2 cut(s) 865, 897
BpmI CTGGAG 3 cut(s) 591, 867, 1173
Bpu10I CCTNAGC 2 cut(s) 17, 705
BpuEI CTTGAG 1 cut(s) 666
BsaHI GRCGYC 2 cut(s) 332, 1074
BsaI GGTCTC 1 cut(s) 220
BsaJI CCNNGG 5 cut(s) 488, 645, 912, 1316, 1660
BsaXI ACNNNNNCTCC 4 cut(s) 633, 663, 1182, 1212
Bsc4I CCNNNNNNNGG 2 cut(s) 833, 1666
Bse118I RCCGGY 2 cut(s) 436, 826
Bse1I ACTGG 1 cut(s) 1681
Bse3DI GCAATG 6 cut(s) 583, 585, 597, 806, 933, 1455
BseBI CCWGG 3 cut(s) 350, 1317, 1596
BseDI CCNNGG 5 cut(s) 488, 645, 912, 1316, 1660
BseGI GGATG 1 cut(s) 922
BseLI CCNNNNNNNGG 2 cut(s) 833, 1666
BseMI GCAATG 6 cut(s) 583, 585, 597, 806, 933, 1455
BseMII CTCAG 3 cut(s) 36, 138, 1232
BseNI ACTGG 1 cut(s) 1681
BseRI GAGGAG 5 cut(s) 157, 160, 163, 725, 1121
BseSI GKGCMC 2 cut(s) 1141, 1428
BseXI GCAGC 2 cut(s) 445, 847
Bsh1236I CGCG 1 cut(s) 1662
BshFI GGCC 4 cut(s) 8, 843, 1350, 1594
BshNI GGYRCC 2 cut(s) 1136, 1312
BsiHKAI GWGCWC 1 cut(s) 1428
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 2 cut(s) 437, 827
BsiWI CGTACG 1 cut(s) 269
BslFI GGGAC 5 cut(s) 16, 84, 88, 118, 729
BslI CCNNNNNNNGG 2 cut(s) 833, 1666
BsmAI GTCTC 3 cut(s) 220, 885, 1103
BsmFI GGGAC 5 cut(s) 16, 84, 88, 118, 729
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 4 cut(s) 8, 843, 1350, 1594
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 912, 1141, 1428
Bsp1407I TGTACA 1 cut(s) 1410
Bsp143I GATC 4 cut(s) 311, 739, 1485, 1516
BspACI CCGC 6 cut(s) 156, 394, 558, 1215, 1660, 1662
BspANI GGCC 4 cut(s) 8, 843, 1350, 1594
BspCNI CTCAG 3 cut(s) 35, 139, 1233
BspFNI CGCG 1 cut(s) 1662
BspLI GGNNCC 6 cut(s) 76, 77, 643, 909, 1138, 1314
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 1 cut(s) 319
BspT107I GGYRCC 2 cut(s) 1136, 1312
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 394
BsrDI GCAATG 6 cut(s) 583, 585, 597, 806, 933, 1455
BsrFI RCCGGY 2 cut(s) 436, 826
BsrGI TGTACA 1 cut(s) 1410
BsrI ACTGG 1 cut(s) 1681
BssAI RCCGGY 2 cut(s) 436, 826
BssECI CCNNGG 5 cut(s) 488, 645, 912, 1316, 1660
BssMI GATC 4 cut(s) 311, 739, 1485, 1516
BssNI GRCGYC 2 cut(s) 332, 1074
BssSI CACGAG 2 cut(s) 1061, 1737
BssT1I CCWWGG 1 cut(s) 488
Bst2BI CACGAG 2 cut(s) 1061, 1737
Bst2UI CCWGG 3 cut(s) 350, 1317, 1596
BstACI GRCGYC 2 cut(s) 332, 1074
BstAPI GCANNNNNTGC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 1410
BstC8I GCNNGC 3 cut(s) 375, 702, 813
BstDEI CTNAG 7 cut(s) 17, 22, 147, 480, 693, 705, 1241
BstDSI CCRYGG 2 cut(s) 645, 1660
BstF5I GGATG 1 cut(s) 922
BstFNI CGCG 1 cut(s) 1662
BstHHI GCGC 2 cut(s) 541, 1461
BstKTI GATC 4 cut(s) 314, 742, 1488, 1519
BstMAI GTCTC 3 cut(s) 220, 885, 1103
BstMBI GATC 4 cut(s) 311, 739, 1485, 1516
BstMWI GCNNNNNNNGC 8 cut(s) 370, 391, 557, 587, 857, 1292, 1312, 1801
BstNI CCWGG 3 cut(s) 350, 1317, 1596
BstNSI RCATGY 2 cut(s) 815, 1102
BstSCI CCNGG 3 cut(s) 348, 1315, 1594
BstSLI GKGCMC 2 cut(s) 1141, 1428
BstUI CGCG 1 cut(s) 1662
BstV1I GCAGC 2 cut(s) 445, 847
BstV2I GAAGAC 2 cut(s) 1461, 1573
BstX2I RGATCY 1 cut(s) 311
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 311
BsuRI GGCC 4 cut(s) 8, 843, 1350, 1594
BtgI CCRYGG 2 cut(s) 645, 1660
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 922
BtsI GCAGTG 2 cut(s) 120, 1005
BtsIMutI CAGTG 3 cut(s) 120, 1005, 1420
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 3 cut(s) 375, 702, 813
CaiI CAGNNNCTG 2 cut(s) 263, 1436
CfoI GCGC 2 cut(s) 541, 1461
Cfr10I RCCGGY 2 cut(s) 436, 826
Cfr13I GGNCC 4 cut(s) 7, 75, 967, 1349
Cfr42I CCGCGG 1 cut(s) 1663
CseI GACGC 3 cut(s) 311, 779, 1082
Csp6I GTAC 6 cut(s) 260, 270, 653, 1289, 1406, 1411
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 4 cut(s) 536, 812, 1099, 1462
CviQI GTAC 6 cut(s) 260, 270, 653, 1289, 1406, 1411
DdeI CTNAG 7 cut(s) 17, 22, 147, 480, 693, 705, 1241
DpnI GATC 4 cut(s) 313, 741, 1487, 1518
DpnII GATC 4 cut(s) 311, 739, 1485, 1516
DraI TTTAAA 1 cut(s) 1327
EciI GGCGGA 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 488
Eco147I AGGCCT 1 cut(s) 1594
Eco24I GRGCYC 1 cut(s) 912
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 2 cut(s) 75, 967
Eco57I CTGAAG 1 cut(s) 1179
Eco88I CYCGRG 1 cut(s) 71
EcoO109I RGGNCCY 2 cut(s) 7, 75
EcoRI GAATTC 1 cut(s) 1367
EcoRII CCWGG 3 cut(s) 348, 1315, 1594
EcoT14I CCWWGG 1 cut(s) 488
EcoT22I ATGCAT 1 cut(s) 1806
EcoT38I GRGCYC 1 cut(s) 912
ErhI CCWWGG 1 cut(s) 488
FaeI CATG 4 cut(s) 539, 815, 1102, 1465
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 5 cut(s) 16, 84, 88, 118, 729
FatI CATG 4 cut(s) 535, 811, 1098, 1461
FauI CCCGC 1 cut(s) 1208
FbaI TGATCA 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 434, 861
FokI GGATG 1 cut(s) 929
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 2 cut(s) 434, 861
FspBI CTAG 3 cut(s) 222, 279, 1113
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 2 cut(s) 540, 1460
GluI GCNGC 2 cut(s) 434, 861
GsuI CTGGAG 3 cut(s) 591, 867, 1173
HaeIII GGCC 4 cut(s) 8, 843, 1350, 1594
HapII CCGG 2 cut(s) 437, 827
HgaI GACGC 3 cut(s) 311, 779, 1082
HhaI GCGC 2 cut(s) 541, 1461
Hin1I GRCGYC 2 cut(s) 332, 1074
Hin1II CATG 4 cut(s) 539, 815, 1102, 1465
Hin6I GCGC 2 cut(s) 539, 1459
HinP1I GCGC 2 cut(s) 539, 1459
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 2 cut(s) 452, 684
HinfI GANTC 7 cut(s) 59, 190, 359, 484, 665, 1466, 1698
HpaII CCGG 2 cut(s) 437, 827
HphI GGTGA 7 cut(s) 5, 674, 734, 1025, 1261, 1618, 1682
Hpy166II GTNNAC 4 cut(s) 301, 406, 1426, 1631
Hpy188I TCNGA 6 cut(s) 391, 776, 916, 1029, 1390, 1438
Hpy188III TCNNGA 7 cut(s) 73, 135, 187, 608, 884, 1063, 1495
Hpy8I GTNNAC 4 cut(s) 301, 406, 1426, 1631
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 1 cut(s) 20
HpyCH4IV ACGT 3 cut(s) 332, 867, 1633
HpyF10VI GCNNNNNNNGC 8 cut(s) 370, 391, 557, 587, 857, 1292, 1312, 1801
HpyF3I CTNAG 7 cut(s) 17, 22, 147, 480, 693, 705, 1241
HpySE526I ACGT 3 cut(s) 332, 867, 1633
Hsp92I GRCGYC 2 cut(s) 332, 1074
Hsp92II CATG 4 cut(s) 539, 815, 1102, 1465
HspAI GCGC 2 cut(s) 539, 1459
KflI GGGWCCC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 739
KspI CCGCGG 1 cut(s) 1663
Kzo9I GATC 4 cut(s) 311, 739, 1485, 1516
LmnI GCTCC 6 cut(s) 461, 641, 720, 907, 1154, 1320
Lsp1109I GCAGC 2 cut(s) 445, 847
LweI GCATC 4 cut(s) 188, 907, 952, 1791
MaeI CTAG 3 cut(s) 222, 279, 1113
MaeII ACGT 3 cut(s) 332, 867, 1633
MaeIII GTNAC 6 cut(s) 598, 1070, 1249, 1502, 1526, 1771
MalI GATC 4 cut(s) 313, 741, 1487, 1518
MbiI CCGCTC 1 cut(s) 394
MboI GATC 4 cut(s) 311, 739, 1485, 1516
MboII GAAGA 4 cut(s) 437, 1028, 1461, 1573
MfeI CAATTG 2 cut(s) 354, 497
MflI RGATCY 1 cut(s) 311
MhlI GDGCHC 3 cut(s) 912, 1141, 1428
MluCI AATT 9 cut(s) 354, 497, 566, 612, 816, 1040, 1206, 1367, 1744
MlyI GAGTC 2 cut(s) 184, 1475
MmeI TCCRAC 5 cut(s) 754, 945, 955, 1616, 1675
Mph1103I ATGCAT 1 cut(s) 1806
MroXI GAANNNNTTC 1 cut(s) 1023
MseI TTAA 5 cut(s) 615, 870, 1185, 1326, 1512
MslI CAYNNNNRTG 4 cut(s) 590, 755, 1335, 1737
MspA1I CMGCKG 3 cut(s) 158, 1215, 1662
MspI CCGG 2 cut(s) 437, 827
MspR9I CCNGG 3 cut(s) 350, 1317, 1596
MunI CAATTG 2 cut(s) 354, 497
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 3 cut(s) 350, 1317, 1596
MvnI CGCG 1 cut(s) 1662
MwoI GCNNNNNNNGC 8 cut(s) 370, 391, 557, 587, 857, 1292, 1312, 1801
NdeII GATC 4 cut(s) 311, 739, 1485, 1516
NlaIII CATG 4 cut(s) 539, 815, 1102, 1465
NlaIV GGNNCC 6 cut(s) 76, 77, 643, 909, 1138, 1314
NmuCI GTSAC 4 cut(s) 1070, 1249, 1502, 1771
NsbI TGCGCA 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 1806
NspI RCATGY 2 cut(s) 815, 1102
PaeI GCATGC 1 cut(s) 815
PaqCI CACCTGC 1 cut(s) 1124
PceI AGGCCT 1 cut(s) 1594
PciI ACATGT 1 cut(s) 1098
PctI GAATGC 1 cut(s) 928
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 5 cut(s) 59, 359, 484, 665, 1698
Pfl23II CGTACG 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 193
PkrI GCNGC 2 cut(s) 435, 862
PleI GAGTC 2 cut(s) 184, 1474
PpsI GAGTC 2 cut(s) 184, 1474
PpuMI RGGWCCY 1 cut(s) 75
PscI ACATGT 1 cut(s) 1098
PsiI TTATAA 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 3 cut(s) 348, 1315, 1594
PspGI CCWGG 3 cut(s) 348, 1315, 1594
PspLI CGTACG 1 cut(s) 269
PspN4I GGNNCC 6 cut(s) 76, 77, 643, 909, 1138, 1314
PspPI GGNCC 4 cut(s) 7, 75, 967, 1349
PspPPI RGGWCCY 1 cut(s) 75
PstNI CAGNNNCTG 2 cut(s) 263, 1436
PsuI RGATCY 1 cut(s) 311
PsyI GACNNNGTC 1 cut(s) 193
RsaI GTAC 6 cut(s) 261, 271, 654, 1290, 1407, 1412
RsaNI GTAC 6 cut(s) 260, 270, 653, 1289, 1406, 1411
RseI CAYNNNNRTG 4 cut(s) 590, 755, 1335, 1737
SacII CCGCGG 1 cut(s) 1663
SaqAI TTAA 5 cut(s) 615, 870, 1185, 1326, 1512
SatI GCNGC 2 cut(s) 434, 861
Sau3AI GATC 4 cut(s) 311, 739, 1485, 1516
Sau96I GGNCC 4 cut(s) 7, 75, 967, 1349
SchI GAGTC 2 cut(s) 184, 1475
ScrFI CCNGG 3 cut(s) 350, 1317, 1596
SduI GDGCHC 3 cut(s) 912, 1141, 1428
SfaNI GCATC 4 cut(s) 188, 907, 952, 1791
Sfr303I CCGCGG 1 cut(s) 1663
SgrBI CCGCGG 1 cut(s) 1663
SinI GGWCC 2 cut(s) 75, 967
SmiMI CAYNNNNRTG 4 cut(s) 590, 755, 1335, 1737
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
SpeI ACTAGT 1 cut(s) 221
SphI GCATGC 1 cut(s) 815
Sse9I AATT 9 cut(s) 354, 497, 566, 612, 816, 1040, 1206, 1367, 1744
SseBI AGGCCT 1 cut(s) 1594
SsiI CCGC 6 cut(s) 156, 394, 558, 1215, 1660, 1662
SspI AATATT 2 cut(s) 1360, 1642
SspMI CTAG 3 cut(s) 222, 279, 1113
StuI AGGCCT 1 cut(s) 1594
StyD4I CCNGG 3 cut(s) 348, 1315, 1594
StyI CCWWGG 1 cut(s) 488
TaiI ACGT 3 cut(s) 335, 870, 1636
TaqI TCGA 1 cut(s) 1791
TasI AATT 9 cut(s) 354, 497, 566, 612, 816, 1040, 1206, 1367, 1744
TatI WGTACW 3 cut(s) 652, 1288, 1410
TfiI GAWTC 5 cut(s) 59, 359, 484, 665, 1698
Tru1I TTAA 5 cut(s) 615, 870, 1185, 1326, 1512
Tru9I TTAA 5 cut(s) 615, 870, 1185, 1326, 1512
TscAI CASTG 3 cut(s) 127, 1005, 1420
TseFI GTSAC 4 cut(s) 1070, 1249, 1502, 1771
TseI GCWGC 2 cut(s) 433, 860
Tsp45I GTSAC 4 cut(s) 1070, 1249, 1502, 1771
TspDTI ATGAA 5 cut(s) 106, 252, 715, 1241, 1370
TspGWI ACGGA 3 cut(s) 183, 733, 963
TspRI CASTG 3 cut(s) 127, 1005, 1420
Tth111I GACNNNGTC 1 cut(s) 193
VneI GTGCAC 1 cut(s) 1424
VpaK11BI GGWCC 2 cut(s) 75, 967
XapI RAATTY 1 cut(s) 1367
XceI RCATGY 2 cut(s) 815, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 3 cut(s) 222, 279, 1113
ZraI GACGTC 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 1806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.