Rh3DG255700

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000940
Physical Location & Seq
Reverse (-)
25296758 .. 25298730
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG255700.1

Sequence Viewer

Length: 1128 bp
ATGGTGAAACGCGTATTGGGTTGTGCTACTAGTACTAGTAGCGGAGGAGATGAAACGCACGAAACTGATCACGCGACGCCGGCGGTGGCTGACCCTAGAGTACTCCGATCACGCTACCACACTGTCAAGACCATCCTCTACGATGAAAGAGAGGATCTCACAAGAGCAGATTCGGAGAAGTTCAATTCGATCATTAAGGAAGTGGAGAGCTTGCACCAGCTGGTAACAAAGCCAAGAGAGCAAGTCGCCGATGCGGAGGCGCTTTTGGACATTGCAAGCACCCTGTTGACCTCTGTACGGGCACATAACAAGGAAGGAATTACAGCTTCGGGTTTCGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTGATGGCAGCAGCTCTATATCTTGGAGGGACATTGGGAATGCGGTATCGCATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCAATGAATGCTGTTGTGAAGCAACGAAAGGTTTATCAGCGTCAAAAACGTGGGAATCCTACAGACAATGACACCCCTAAAGAGCTTGGTGATGCTGTTGTAGAAGAGGAAACAGAGACTGTTAAAAATGTGGCAACTATGTTTAATATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGGCGAGTCGAAATAAAAGTGAACGAGGAAGGCCATCATCTAGTGTCCCCAAGGAATGCTCCTGCTGCCGAAGCAATTGCCTCAGGCGCTGTTACTTACTGCCACTTAATCTTCAGATTTGATTTCAAGGACTGGAAGTTAATGAAAGAAGTTGTTGGGAATGGGGAGGAGCTGATGCCACATAGGTTTCCAGCTGAAGATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGCTTGGTTATAAGGGAGCAGCCCGTTGGGACTGATAGTAGCAGCAGATCAATTGCTGAAGACAGCACTGAATGTGATTATGCTGCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

375

Amino Acids

41.63

Weight (kDa)

8.14

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 212 - 299 4e-22 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1010
AccII CGCG 2 cut(s) 12, 74
AciI CCGC 4 cut(s) 42, 83, 254, 416
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 4 cut(s) 343, 817, 936, 1077
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 34, 102, 297, 967
AfeI AGCGCT 1 cut(s) 1090
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 5 cut(s) 184, 619, 644, 658, 847
AhlI ACTAGT 2 cut(s) 29, 35
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 986, 1018
AluBI AGCT 7 cut(s) 210, 220, 326, 387, 550, 892, 914
AluI AGCT 7 cut(s) 210, 220, 326, 387, 550, 892, 914
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 2 cut(s) 584, 809
Aor51HI AGCGCT 1 cut(s) 1090
AoxI GGCC 2 cut(s) 464, 751
ApeKI GCWGC 7 cut(s) 381, 384, 450, 785, 1018, 1041, 1082
ApoI RAATTY 1 cut(s) 664
AspLEI GCGC 3 cut(s) 262, 809, 1091
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 3 cut(s) 16, 386, 566
AxyI CCTNAGG 1 cut(s) 802
BaeGI GKGCMC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 1065
BbvI GCAGC 7 cut(s) 393, 396, 437, 772, 1030, 1053, 1069
BccI CCATC 4 cut(s) 140, 371, 713, 762
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 575
BcuI ACTAGT 2 cut(s) 29, 35
BfaI CTAG 5 cut(s) 30, 36, 96, 650, 761
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 3 cut(s) 263, 810, 1092
BisI GCNGC 7 cut(s) 382, 385, 451, 786, 1019, 1042, 1083
BlsI GCNGC 7 cut(s) 383, 386, 452, 787, 1020, 1043, 1084
BmcAI AGTACT 2 cut(s) 34, 102
BmgT120I GGNCC 1 cut(s) 464
BmsI GCATC 4 cut(s) 241, 437, 547, 885
BpiI GAAGAC 1 cut(s) 1065
BplI GAGNNNNNCTC 4 cut(s) 141, 173, 427, 459
BpuEI CTTGAG 2 cut(s) 936, 973
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 770
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 857, 945
Bse21I CCTNAGG 1 cut(s) 802
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 770
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 857, 945
BseRI GAGGAG 2 cut(s) 60, 902
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 7 cut(s) 393, 396, 437, 772, 1030, 1053, 1069
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 12, 74
BshFI GGCC 2 cut(s) 466, 753
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 3 cut(s) 416, 751, 1042
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 3 cut(s) 416, 751, 1042
BsmI GAATGC 3 cut(s) 418, 478, 781
BsnI GGCC 2 cut(s) 466, 753
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 5 cut(s) 67, 107, 154, 189, 1046
BspACI CCGC 4 cut(s) 42, 83, 254, 416
BspANI GGCC 2 cut(s) 466, 753
BspCNI CTCAG 1 cut(s) 815
BspFNI CGCG 2 cut(s) 12, 74
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 857, 945
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 770
BssMI GATC 5 cut(s) 67, 107, 154, 189, 1046
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 124, 344, 586, 682
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 4 cut(s) 81, 212, 277, 1087
BstDEI CTNAG 2 cut(s) 350, 802
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 2 cut(s) 12, 74
BstH2I RGCGCY 3 cut(s) 263, 810, 1092
BstHHI GCGC 3 cut(s) 262, 809, 1091
BstKTI GATC 5 cut(s) 70, 110, 157, 192, 1049
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 67, 107, 154, 189, 1046
BstMWI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1088
BstNSI RCATGY 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 304
BstUI CGCG 2 cut(s) 12, 74
BstV1I GCAGC 7 cut(s) 393, 396, 437, 772, 1030, 1053, 1069
BstV2I GAAGAC 1 cut(s) 1065
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 802
BsuRI GGCC 2 cut(s) 466, 753
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 120, 687, 1065
Cac8I GCNNGC 4 cut(s) 81, 212, 277, 1087
CaiI CAGNNNCTG 2 cut(s) 584, 809
CfoI GCGC 3 cut(s) 262, 809, 1091
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 2 cut(s) 85, 494
Csp6I GTAC 4 cut(s) 33, 101, 296, 966
CviAII CATG 1 cut(s) 425
CviQI GTAC 4 cut(s) 33, 101, 296, 966
DdeI CTNAG 2 cut(s) 350, 802
DpnI GATC 5 cut(s) 69, 109, 156, 191, 1048
DpnII GATC 5 cut(s) 67, 107, 154, 189, 1046
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 770
Eco47III AGCGCT 1 cut(s) 1090
Eco57I CTGAAG 4 cut(s) 343, 817, 936, 1077
Eco81I CCTNAGG 1 cut(s) 802
EcoT14I CCWWGG 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 770
FaeI CATG 1 cut(s) 428
FaiI YATR 9 cut(s) 306, 392, 426, 458, 605, 903, 1010, 1080, 1126
FaqI GGGAC 3 cut(s) 416, 751, 1042
FatI CATG 1 cut(s) 424
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 382, 385, 451, 786, 1019, 1042, 1083
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 7 cut(s) 382, 385, 451, 786, 1019, 1042, 1083
FspBI CTAG 5 cut(s) 30, 36, 96, 650, 761
GlaI GCGC 3 cut(s) 261, 808, 1090
GluI GCNGC 7 cut(s) 382, 385, 451, 786, 1019, 1042, 1083
HaeII RGCGCY 3 cut(s) 263, 810, 1092
HaeIII GGCC 2 cut(s) 466, 753
HapII CCGG 1 cut(s) 80
HgaI GACGC 2 cut(s) 85, 494
HhaI GCGC 3 cut(s) 262, 809, 1091
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 3 cut(s) 260, 807, 1089
HinP1I GCGC 3 cut(s) 260, 807, 1089
HincII GTYRAC 2 cut(s) 288, 340
HindII GTYRAC 2 cut(s) 288, 340
HinfI GANTC 3 cut(s) 170, 520, 726
HpaII CCGG 1 cut(s) 80
HphI GGTGA 3 cut(s) 16, 386, 566
Hpy166II GTNNAC 4 cut(s) 288, 340, 742, 1118
Hpy188I TCNGA 3 cut(s) 107, 175, 836
Hpy188III TCNNGA 3 cut(s) 127, 361, 433
Hpy8I GTNNAC 4 cut(s) 288, 340, 742, 1118
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 308, 624, 743
HpyCH4III ACNGT 4 cut(s) 124, 344, 586, 682
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 4 cut(s) 214, 275, 453, 1085
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1088
HpyF3I CTNAG 2 cut(s) 350, 802
HpySE526I ACGT 1 cut(s) 514
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 3 cut(s) 260, 807, 1089
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 107, 154, 189, 1046
LmnI GCTCC 3 cut(s) 784, 889, 1015
Lsp1109I GCAGC 7 cut(s) 393, 396, 437, 772, 1030, 1053, 1069
LweI GCATC 4 cut(s) 241, 437, 547, 885
MaeI CTAG 5 cut(s) 30, 36, 96, 650, 761
MaeII ACGT 1 cut(s) 514
MaeIII GTNAC 3 cut(s) 223, 353, 811
MalI GATC 5 cut(s) 69, 109, 156, 191, 1048
MboI GATC 5 cut(s) 67, 107, 154, 189, 1046
MboII GAAGA 6 cut(s) 581, 631, 823, 929, 956, 1070
MfeI CAATTG 2 cut(s) 795, 1050
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 7 cut(s) 184, 318, 664, 795, 931, 975, 1050
MluI ACGCGT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 735
MreI CGCCGGCG 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
MspA1I CMGCKG 2 cut(s) 220, 914
MspI CCGG 1 cut(s) 80
MunI CAATTG 2 cut(s) 795, 1050
Mva1269I GAATGC 3 cut(s) 418, 478, 781
MvnI CGCG 2 cut(s) 12, 74
MwoI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1088
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 5 cut(s) 67, 107, 154, 189, 1046
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 1 cut(s) 428
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 428
PctI GAATGC 3 cut(s) 418, 478, 781
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 170, 520
PkrI GCNGC 7 cut(s) 383, 386, 452, 787, 1020, 1043, 1084
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
PsiI TTATAA 1 cut(s) 1010
PspPI GGNCC 1 cut(s) 464
PstNI CAGNNNCTG 2 cut(s) 584, 809
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 220, 914
RsaI GTAC 4 cut(s) 34, 102, 297, 967
RsaNI GTAC 4 cut(s) 33, 101, 296, 966
SaqAI TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
SatI GCNGC 7 cut(s) 382, 385, 451, 786, 1019, 1042, 1083
Sau3AI GATC 5 cut(s) 67, 107, 154, 189, 1046
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 2 cut(s) 34, 102
SchI GAGTC 1 cut(s) 735
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 4 cut(s) 241, 437, 547, 885
SfcI CTRYAG 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 951, 988
SmoI CTYRAG 2 cut(s) 951, 988
SpeI ACTAGT 2 cut(s) 29, 35
Sse9I AATT 7 cut(s) 184, 318, 664, 795, 931, 975, 1050
SsiI CCGC 4 cut(s) 42, 83, 254, 416
SspI AATATT 1 cut(s) 613
SspMI CTAG 5 cut(s) 30, 36, 96, 650, 761
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 4 cut(s) 124, 344, 586, 682
TaiI ACGT 1 cut(s) 517
TaqI TCGA 3 cut(s) 188, 445, 729
TasI AATT 7 cut(s) 184, 318, 664, 795, 931, 975, 1050
TatI WGTACW 2 cut(s) 32, 100
TfiI GAWTC 2 cut(s) 170, 520
Tru1I TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
Tru9I TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
TscAI CASTG 3 cut(s) 127, 687, 1072
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 7 cut(s) 381, 384, 450, 785, 1018, 1041, 1082
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 66, 159, 485, 878
TspRI CASTG 3 cut(s) 127, 687, 1072
XapI RAATTY 1 cut(s) 664
XceI RCATGY 1 cut(s) 428
XspI CTAG 5 cut(s) 30, 36, 96, 650, 761
ZrmI AGTACT 2 cut(s) 34, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.