Rh5DG554700

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000940
Physical Location & Seq
Forward (+)
85685127 .. 85688534
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG554700.1

Sequence Viewer

Length: 915 bp
ATGCCGAGGACCGCGAAACGCGAACGGAGCGCCGTTCAAGGCCGTGGCGTCGACGACGGCGAGGAGGCGGAGCGGCTCCGAGCGGTGAAGCGCGAGCGACTCAATTCCGAGCAGGTTGAAAATGAGTCAACCGATCAAGACGCCGGTCAACGGCGAGCTCTCCGATCTGACTACAATGAAATCCTGAACCTCATTAGTGAGAAGAGGAATGAGTTACTGAAGCCGGAGTCGGAGAAATTTGGCGTCATCATTGGTAGAGTTGAGAAGTTGCATGAGCTAGTTCAAAAGCCAAGAGAGCAAGTGGCAGATGCAGAGGCACTGTTAGACATAACCAACACATTAGTGACCTCTGTTAAGTCACAGTCCAGTGCTGGCATTACCCCATCGGACTTCATTACCTGTTTGATTCGAGATTTTGGGCAATTAAGGGATGGCCATGCCTCGATTCGATGGAAAGACGTTGGGGTTGCTGTGGCGCCAATTTTCAAGAGGGCTCATGGGTGCTGTACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCAGCTGTTCGTACTAGACGAATAAAGCCTACCACAACTGATGAGCCTGAGGAGATTGATGCACAGGAAGAAGAAAAAACTGATACGGATAGGAATATGTCAACGATGTTTGAGATATTAAGAAAGAAGAAACGTGTCCGACTGGAATGTTTAATATTAAACAGAATGTTCTTTGCACAGACAGTCGAGAATTTATTTGCTCTTTCATTCCTTGTTAAAGATGGCCGGGCTGAAATCATAGTTGATGCAAATCGCGTTCATTTTGTTGCACCAAGGAATGCTGCCAAGGCCACTGAAGTGGCATCTCGTGAGGTTGTTTATAGACACTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

304

Amino Acids

34.88

Weight (kDa)

8.81

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 229 - 303 2.2e-18 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 103
AccB1I GGYRCC 1 cut(s) 475
AccBSI CCGCTC 2 cut(s) 73, 83
AccI GTMKAC 1 cut(s) 51
AccII CGCG 4 cut(s) 14, 21, 93, 804
AciI CCGC 4 cut(s) 12, 68, 73, 83
AcoI YGGCCR 2 cut(s) 433, 772
AcsI RAATTY 2 cut(s) 236, 739
AcuI CTGAAG 2 cut(s) 239, 864
AcyI GRCGYC 4 cut(s) 48, 141, 243, 476
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 2 cut(s) 508, 562
AfiI CCNNNNNNNGG 1 cut(s) 150
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 5 cut(s) 38, 119, 284, 487, 898
AleI CACNNNNGTG 2 cut(s) 341, 845
AluBI AGCT 3 cut(s) 158, 277, 554
AluI AGCT 3 cut(s) 158, 277, 554
Alw21I GWGCWC 1 cut(s) 160
AoxI GGCC 4 cut(s) 40, 433, 772, 837
ApeKI GCWGC 2 cut(s) 551, 830
ApoI RAATTY 2 cut(s) 236, 739
AspLEI GCGC 3 cut(s) 32, 93, 478
AspS9I GGNCC 2 cut(s) 9, 518
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 9, 518
AxyI CCTNAGG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 2 cut(s) 160, 496
BauI CACGAG 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 563, 817
BccI CCATC 4 cut(s) 391, 425, 444, 764
BceAI ACGGC 4 cut(s) 17, 27, 73, 167
BcnI CCSGG 1 cut(s) 776
BfaI CTAG 2 cut(s) 278, 564
BfoI RGCGCY 2 cut(s) 33, 479
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 3 cut(s) 74, 552, 831
BlsI GCNGC 3 cut(s) 75, 553, 832
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 2 cut(s) 9, 518
BmgT120I GGNCC 2 cut(s) 9, 518
BmiI GGNNCC 3 cut(s) 77, 477, 520
BmrFI CCNGG 1 cut(s) 776
BmsI GCATC 4 cut(s) 298, 598, 784, 860
BoxI GACNNNNGTC 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 4 cut(s) 48, 141, 243, 476
BsaJI CCNNGG 4 cut(s) 5, 43, 821, 834
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 366, 696, 908
Bse21I CCTNAGG 1 cut(s) 597
Bse8I GATNNNNATC 1 cut(s) 798
BseDI CCNNGG 4 cut(s) 5, 43, 821, 834
BseGI GGATG 1 cut(s) 436
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMII CTCAG 2 cut(s) 522, 588
BseNI ACTGG 3 cut(s) 366, 696, 908
BseRI GAGGAG 2 cut(s) 77, 614
BseXI GCAGC 2 cut(s) 563, 817
Bsh1236I CGCG 4 cut(s) 14, 21, 93, 804
BshFI GGCC 4 cut(s) 42, 435, 774, 839
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 3 cut(s) 144, 224, 775
BslI CCNNNNNNNGG 1 cut(s) 150
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 4 cut(s) 42, 435, 774, 839
Bsp1286I GDGCHC 2 cut(s) 160, 496
Bsp1407I TGTACA 1 cut(s) 506
Bsp143I GATC 2 cut(s) 133, 164
BspACI CCGC 4 cut(s) 12, 68, 73, 83
BspANI GGCC 4 cut(s) 42, 435, 774, 839
BspCNI CTCAG 2 cut(s) 523, 589
BspFNI CGCG 4 cut(s) 14, 21, 93, 804
BspLI GGNNCC 3 cut(s) 77, 477, 520
BspMI ACCTGC 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 475
BsrBI CCGCTC 2 cut(s) 73, 83
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 506
BsrI ACTGG 3 cut(s) 366, 696, 908
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 4 cut(s) 5, 43, 821, 834
BssMI GATC 2 cut(s) 133, 164
BssNI GRCGYC 4 cut(s) 48, 141, 243, 476
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 821, 834
Bst2BI CACGAG 1 cut(s) 855
Bst4CI ACNGT 3 cut(s) 321, 363, 733
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 4 cut(s) 48, 141, 243, 476
BstAUI TGTACA 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 95, 156, 373
BstDEI CTNAG 2 cut(s) 531, 597
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 4 cut(s) 14, 21, 93, 804
BstH2I RGCGCY 2 cut(s) 33, 479
BstHHI GCGC 3 cut(s) 32, 93, 478
BstKTI GATC 2 cut(s) 136, 167
BstMBI GATC 2 cut(s) 133, 164
BstMWI GCNNNNNNNGC 3 cut(s) 27, 295, 836
BstPAI GACNNNNGTC 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 774
BstUI CGCG 4 cut(s) 14, 21, 93, 804
BstV1I GCAGC 2 cut(s) 563, 817
BstXI CCANNNNNNTGG 1 cut(s) 847
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 4 cut(s) 42, 435, 774, 839
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 317, 373, 840
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 3 cut(s) 95, 156, 373
CfoI GCGC 3 cut(s) 32, 93, 478
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 9, 518
CseI GACGC 3 cut(s) 37, 149, 232
Csp6I GTAC 2 cut(s) 507, 561
CviAII CATG 4 cut(s) 272, 437, 497, 523
CviQI GTAC 2 cut(s) 507, 561
DdeI CTNAG 2 cut(s) 531, 597
DinI GGCGCC 1 cut(s) 477
DpnI GATC 2 cut(s) 135, 166
DpnII GATC 2 cut(s) 133, 164
DraIII CACNNNGTG 1 cut(s) 880
EaeI YGGCCR 2 cut(s) 433, 772
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 821, 834
Eco24I GRGCYC 2 cut(s) 160, 496
Eco47I GGWCC 2 cut(s) 9, 518
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 2 cut(s) 239, 864
Eco81I CCTNAGG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 158
EcoT14I CCWWGG 2 cut(s) 821, 834
EcoT38I GRGCYC 2 cut(s) 160, 496
EgeI GGCGCC 1 cut(s) 477
EheI GGCGCC 1 cut(s) 477
ErhI CCWWGG 2 cut(s) 821, 834
FaeI CATG 4 cut(s) 275, 440, 500, 526
FatI CATG 4 cut(s) 271, 436, 496, 522
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 74, 552, 831
FokI GGATG 1 cut(s) 443
FriOI GRGCYC 2 cut(s) 160, 496
Fsp4HI GCNGC 3 cut(s) 74, 552, 831
FspBI CTAG 2 cut(s) 278, 564
GlaI GCGC 3 cut(s) 31, 92, 477
GluI GCNGC 3 cut(s) 74, 552, 831
HaeII RGCGCY 2 cut(s) 33, 479
HaeIII GGCC 4 cut(s) 42, 435, 774, 839
HapII CCGG 3 cut(s) 144, 224, 775
HgaI GACGC 3 cut(s) 37, 149, 232
HhaI GCGC 3 cut(s) 32, 93, 478
Hin1I GRCGYC 4 cut(s) 48, 141, 243, 476
Hin1II CATG 4 cut(s) 275, 440, 500, 526
Hin6I GCGC 3 cut(s) 30, 91, 476
HinP1I GCGC 3 cut(s) 30, 91, 476
HincII GTYRAC 4 cut(s) 52, 129, 149, 651
HindII GTYRAC 4 cut(s) 52, 129, 149, 651
HinfI GANTC 5 cut(s) 99, 125, 227, 406, 445
HpaII CCGG 3 cut(s) 144, 224, 775
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 4 cut(s) 52, 129, 149, 651
Hpy188I TCNGA 8 cut(s) 80, 109, 164, 169, 232, 388, 689, 887
Hpy188III TCNNGA 6 cut(s) 137, 184, 410, 487, 736, 857
Hpy8I GTNNAC 4 cut(s) 52, 129, 149, 651
Hpy99I CGWCG 3 cut(s) 53, 56, 59
HpyAV CCTTC 2 cut(s) 541, 901
HpyCH4III ACNGT 3 cut(s) 321, 363, 733
HpyCH4IV ACGT 2 cut(s) 459, 682
HpyCH4V TGCA 6 cut(s) 271, 311, 611, 725, 797, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 295, 836
HpyF3I CTNAG 2 cut(s) 531, 597
HpySE526I ACGT 2 cut(s) 459, 682
Hsp92I GRCGYC 4 cut(s) 48, 141, 243, 476
Hsp92II CATG 4 cut(s) 275, 440, 500, 526
HspAI GCGC 3 cut(s) 30, 91, 476
KasI GGCGCC 1 cut(s) 475
Kzo9I GATC 2 cut(s) 133, 164
LmnI GCTCC 3 cut(s) 27, 70, 81
Lsp1109I GCAGC 2 cut(s) 563, 817
LweI GCATC 4 cut(s) 298, 598, 784, 860
MaeI CTAG 2 cut(s) 278, 564
MaeII ACGT 2 cut(s) 459, 682
MaeIII GTNAC 3 cut(s) 213, 343, 357
MalI GATC 2 cut(s) 135, 166
MbiI CCGCTC 2 cut(s) 73, 83
MboI GATC 2 cut(s) 133, 164
MboII GAAGA 4 cut(s) 214, 629, 632, 688
MhlI GDGCHC 2 cut(s) 160, 496
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 103, 236, 422, 480, 739
MluNI TGGCCA 1 cut(s) 435
Mly113I GGCGCC 1 cut(s) 476
MlyI GAGTC 3 cut(s) 93, 134, 236
MmeI TCCRAC 2 cut(s) 210, 712
Mox20I TGGCCA 1 cut(s) 435
MscI TGGCCA 1 cut(s) 435
MseI TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
MslI CAYNNNNRTG 2 cut(s) 341, 845
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 3 cut(s) 144, 224, 775
MspR9I CCNGG 1 cut(s) 776
Mva1269I GAATGC 1 cut(s) 832
MvnI CGCG 4 cut(s) 14, 21, 93, 804
MwoI GCNNNNNNNGC 3 cut(s) 27, 295, 836
NarI GGCGCC 1 cut(s) 476
NciI CCSGG 1 cut(s) 776
NdeII GATC 2 cut(s) 133, 164
NlaIII CATG 4 cut(s) 275, 440, 500, 526
NlaIV GGNNCC 3 cut(s) 77, 477, 520
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 343, 357
OliI CACNNNNGTG 2 cut(s) 341, 845
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PctI GAATGC 1 cut(s) 832
PfeI GAWTC 2 cut(s) 406, 445
PkrI GCNGC 3 cut(s) 75, 553, 832
PleI GAGTC 3 cut(s) 93, 133, 235
PluTI GGCGCC 1 cut(s) 479
PpsI GAGTC 3 cut(s) 93, 133, 235
PshAI GACNNNNGTC 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 160
PspN4I GGNNCC 3 cut(s) 77, 477, 520
PspPI GGNCC 2 cut(s) 9, 518
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 2 cut(s) 508, 562
RsaNI GTAC 2 cut(s) 507, 561
RseI CAYNNNNRTG 2 cut(s) 341, 845
SacI GAGCTC 1 cut(s) 160
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
SatI GCNGC 3 cut(s) 74, 552, 831
Sau3AI GATC 2 cut(s) 133, 164
Sau96I GGNCC 2 cut(s) 9, 518
SchI GAGTC 3 cut(s) 93, 134, 236
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 2 cut(s) 160, 496
SfaNI GCATC 4 cut(s) 298, 598, 784, 860
SfoI GGCGCC 1 cut(s) 477
SgrDI CGTCGACG 1 cut(s) 50
SinI GGWCC 2 cut(s) 9, 518
SmiMI CAYNNNNRTG 2 cut(s) 341, 845
Sse9I AATT 5 cut(s) 103, 236, 422, 480, 739
SsiI CCGC 4 cut(s) 12, 68, 73, 83
SspDI GGCGCC 1 cut(s) 475
SspI AATATT 1 cut(s) 705
SspMI CTAG 2 cut(s) 278, 564
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 2 cut(s) 821, 834
TaaI ACNGT 3 cut(s) 321, 363, 733
TaiI ACGT 2 cut(s) 462, 685
TaqI TCGA 5 cut(s) 51, 409, 443, 448, 735
TasI AATT 5 cut(s) 103, 236, 422, 480, 739
TatI WGTACW 1 cut(s) 506
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 2 cut(s) 406, 445
Tru1I TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
Tru9I TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
TscAI CASTG 3 cut(s) 324, 373, 847
TseFI GTSAC 2 cut(s) 343, 357
TseI GCWGC 2 cut(s) 551, 830
Tsp45I GTSAC 2 cut(s) 343, 357
TspDTI ATGAA 5 cut(s) 192, 382, 539, 744, 797
TspGWI ACGGA 2 cut(s) 40, 650
TspRI CASTG 3 cut(s) 324, 373, 847
VpaK11BI GGWCC 2 cut(s) 9, 518
XapI RAATTY 2 cut(s) 236, 739
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 2 cut(s) 278, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.