Rmu_sc0004329.1_g000005

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004329.1
OG0000940
Physical Location & Seq
Forward (+)
12491 .. 14461
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004329.1_g000005.1.cds

Sequence Viewer

Length: 1149 bp
atggtgaaacgcgtattgggttgtgctactagtactagtagcggaggagatgaaacgcacgaaactgatcacgagacgccggcggtggctgaccctagagtactccgatcacgctaccacactgtcaagaccatcctctacgatgaaagagaggatctcacaagagcagattcggagaagttcaattcgatcattaaggaagtggagagcttgcaccagctggtaacaaagccaagagagcaagtcgcggatgcggaggcgcttttggacattgcaagcaccctgatgacctctgtacgggcacataacaaggaaggaattacagcttcgggtttcgtcaactgtattcttagtgacttcaggaaaccgagtggtaatggcagcagctctatatcttggagggacattgggaatgcggtatcgcatgtttttcagagaagccctcgatgctgcactatgattgggccaatgaatgctgttgtgaagcaacgaaaggtttatcaacgtcaaaaacgtgggaatcctacagacaatgatacccctaaagagcttggtgatgctgttgttgaagaggaaacagagactgttaaaaatgtggcaactatgtttgatattttgaagaaaaagagaagggtcaggcttgaatatctagttttgaatggaaattctttttcacagacagtggaaaatgtgtttgccctatcgtttcttgttaaagatgggcgagtcgaaataaaagtgaacgaggaaggccatcatctgtttttctctgtgtttggtatgtcagcgccaaggaatgctcctgctgccgaagcaattgcctcaggcgctgttacttactgccacttaatcttcagatttgatttcaaggactggaagttaatgaaagaagttgttgggaatggggaggagctgatgccacataggtttccagctgacgatgtatcaaacaattcgcaactggaagaacctcaagcaacaacaggtacaacaccaattaggaagttctcaaggaacagaggcttggttataagggagcagcccgttgggactgatagtagcagcagatcaattgctgaagacagcgctgaatgcgattatgctacaagcgcttctgccactcgtaaagggaagcgtaaactgatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

382

Amino Acids

42.48

Weight (kDa)

7.66

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
AccII CGCG 2 cut(s) 12, 248
AciI CCGC 5 cut(s) 42, 83, 248, 254, 416
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 3 cut(s) 343, 838, 1098
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 34, 102, 297, 988
AfeI AGCGCT 2 cut(s) 1087, 1111
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 6 cut(s) 184, 569, 619, 644, 658, 868
AhlI ACTAGT 2 cut(s) 29, 35
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 1007, 1039
AluBI AGCT 7 cut(s) 210, 220, 326, 387, 550, 913, 935
AluI AGCT 7 cut(s) 210, 220, 326, 387, 550, 913, 935
Alw26I GTCTC 2 cut(s) 68, 575
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 2 cut(s) 584, 830
Aor51HI AGCGCT 2 cut(s) 1087, 1111
AoxI GGCC 2 cut(s) 464, 751
ApeKI GCWGC 6 cut(s) 381, 384, 450, 806, 1039, 1062
ApoI RAATTY 1 cut(s) 664
AspLEI GCGC 5 cut(s) 262, 790, 830, 1088, 1112
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 16, 566
AxyI CCTNAGG 1 cut(s) 823
BaeGI GKGCMC 1 cut(s) 304
BauI CACGAG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 1086
BbvI GCAGC 6 cut(s) 393, 396, 437, 793, 1051, 1074
BccI CCATC 3 cut(s) 140, 713, 762
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 2 cut(s) 68, 575
BcuI ACTAGT 2 cut(s) 29, 35
BfaI CTAG 4 cut(s) 30, 36, 96, 650
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 5 cut(s) 263, 791, 831, 1089, 1113
BisI GCNGC 6 cut(s) 382, 385, 451, 807, 1040, 1063
BlsI GCNGC 6 cut(s) 383, 386, 452, 808, 1041, 1064
BmcAI AGTACT 2 cut(s) 34, 102
BmgT120I GGNCC 1 cut(s) 464
BmsI GCATC 4 cut(s) 241, 437, 547, 906
BpiI GAAGAC 1 cut(s) 1086
BplI GAGNNNNNCTC 4 cut(s) 141, 173, 427, 459
BpuEI CTTGAG 2 cut(s) 957, 994
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 791
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 878, 966
Bse21I CCTNAGG 1 cut(s) 823
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 791
BseGI GGATG 2 cut(s) 132, 256
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 2 cut(s) 878, 966
BseRI GAGGAG 2 cut(s) 60, 923
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 6 cut(s) 393, 396, 437, 793, 1051, 1074
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 12, 248
BshFI GGCC 2 cut(s) 466, 753
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 2 cut(s) 416, 1063
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 68, 575
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 416, 1063
BsmI GAATGC 4 cut(s) 418, 478, 802, 1097
BsnI GGCC 2 cut(s) 466, 753
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 5 cut(s) 67, 107, 154, 189, 1067
BspACI CCGC 5 cut(s) 42, 83, 248, 254, 416
BspANI GGCC 2 cut(s) 466, 753
BspCNI CTCAG 1 cut(s) 836
BspFNI CGCG 2 cut(s) 12, 248
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 878, 966
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 791
BssMI GATC 5 cut(s) 67, 107, 154, 189, 1067
BssNI GRCGYC 1 cut(s) 77
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 791
Bst2BI CACGAG 1 cut(s) 71
Bst4CI ACNGT 4 cut(s) 124, 344, 586, 682
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 81, 212, 277
BstDEI CTNAG 2 cut(s) 350, 823
BstF5I GGATG 2 cut(s) 132, 256
BstFNI CGCG 2 cut(s) 12, 248
BstH2I RGCGCY 5 cut(s) 263, 791, 831, 1089, 1113
BstHHI GCGC 5 cut(s) 262, 790, 830, 1088, 1112
BstKTI GATC 5 cut(s) 70, 110, 157, 192, 1070
BstMAI GTCTC 2 cut(s) 68, 575
BstMBI GATC 5 cut(s) 67, 107, 154, 189, 1067
BstMWI GCNNNNNNNGC 7 cut(s) 238, 447, 806, 812, 827, 1092, 1109
BstNSI RCATGY 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 304
BstUI CGCG 2 cut(s) 12, 248
BstV1I GCAGC 6 cut(s) 393, 396, 437, 793, 1051, 1074
BstV2I GAAGAC 1 cut(s) 1086
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 823
BsuRI GGCC 2 cut(s) 466, 753
BtsCI GGATG 2 cut(s) 132, 256
BtsIMutI CAGTG 2 cut(s) 120, 687
Cac8I GCNNGC 3 cut(s) 81, 212, 277
CaiI CAGNNNCTG 2 cut(s) 584, 830
CfoI GCGC 5 cut(s) 262, 790, 830, 1088, 1112
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 33, 101, 296, 987
CviAII CATG 1 cut(s) 425
CviQI GTAC 4 cut(s) 33, 101, 296, 987
DdeI CTNAG 2 cut(s) 350, 823
DpnI GATC 5 cut(s) 69, 109, 156, 191, 1069
DpnII GATC 5 cut(s) 67, 107, 154, 189, 1067
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 791
Eco47III AGCGCT 2 cut(s) 1087, 1111
Eco57I CTGAAG 3 cut(s) 343, 838, 1098
Eco81I CCTNAGG 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 791
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 1 cut(s) 428
FaqI GGGAC 2 cut(s) 416, 1063
FatI CATG 1 cut(s) 424
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 6 cut(s) 382, 385, 451, 807, 1040, 1063
FokI GGATG 2 cut(s) 119, 263
Fsp4HI GCNGC 6 cut(s) 382, 385, 451, 807, 1040, 1063
FspBI CTAG 4 cut(s) 30, 36, 96, 650
GlaI GCGC 5 cut(s) 261, 789, 829, 1087, 1111
GluI GCNGC 6 cut(s) 382, 385, 451, 807, 1040, 1063
HaeII RGCGCY 5 cut(s) 263, 791, 831, 1089, 1113
HaeIII GGCC 2 cut(s) 466, 753
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 85
HhaI GCGC 5 cut(s) 262, 790, 830, 1088, 1112
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 5 cut(s) 260, 788, 828, 1086, 1110
HinP1I GCGC 5 cut(s) 260, 788, 828, 1086, 1110
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 3 cut(s) 170, 520, 726
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 16, 566
Hpy166II GTNNAC 3 cut(s) 340, 742, 1139
Hpy188I TCNGA 4 cut(s) 107, 175, 435, 857
Hpy188III TCNNGA 3 cut(s) 71, 127, 361
Hpy8I GTNNAC 3 cut(s) 340, 742, 1139
HpyAV CCTTC 3 cut(s) 308, 624, 743
HpyCH4III ACNGT 4 cut(s) 124, 344, 586, 682
HpyCH4IV ACGT 2 cut(s) 505, 514
HpyCH4V TGCA 3 cut(s) 214, 275, 453
HpyF10VI GCNNNNNNNGC 7 cut(s) 238, 447, 806, 812, 827, 1092, 1109
HpyF3I CTNAG 2 cut(s) 350, 823
HpySE526I ACGT 2 cut(s) 505, 514
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 5 cut(s) 260, 788, 828, 1086, 1110
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 107, 154, 189, 1067
LmnI GCTCC 3 cut(s) 805, 910, 1036
Lsp1109I GCAGC 6 cut(s) 393, 396, 437, 793, 1051, 1074
LweI GCATC 4 cut(s) 241, 437, 547, 906
MaeI CTAG 4 cut(s) 30, 36, 96, 650
MaeII ACGT 2 cut(s) 505, 514
MaeIII GTNAC 3 cut(s) 223, 353, 832
MalI GATC 5 cut(s) 69, 109, 156, 191, 1069
MboI GATC 5 cut(s) 67, 107, 154, 189, 1067
MboII GAAGA 5 cut(s) 581, 631, 844, 977, 1091
MfeI CAATTG 2 cut(s) 816, 1071
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 7 cut(s) 184, 318, 664, 816, 952, 996, 1071
MluI ACGCGT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 735
MreI CGCCGGCG 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 5 cut(s) 195, 588, 714, 848, 881
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 220, 935
MspI CCGG 1 cut(s) 80
MunI CAATTG 2 cut(s) 816, 1071
Mva1269I GAATGC 4 cut(s) 418, 478, 802, 1097
MvnI CGCG 2 cut(s) 12, 248
MwoI GCNNNNNNNGC 7 cut(s) 238, 447, 806, 812, 827, 1092, 1109
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 5 cut(s) 67, 107, 154, 189, 1067
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 1 cut(s) 428
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PctI GAATGC 4 cut(s) 418, 478, 802, 1097
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 170, 520
PkrI GCNGC 6 cut(s) 383, 386, 452, 808, 1041, 1064
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
PsiI TTATAA 1 cut(s) 1031
PspPI GGNCC 1 cut(s) 464
PstNI CAGNNNCTG 2 cut(s) 584, 830
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 220, 935
RsaI GTAC 4 cut(s) 34, 102, 297, 988
RsaNI GTAC 4 cut(s) 33, 101, 296, 987
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 5 cut(s) 195, 588, 714, 848, 881
SatI GCNGC 6 cut(s) 382, 385, 451, 807, 1040, 1063
Sau3AI GATC 5 cut(s) 67, 107, 154, 189, 1067
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 2 cut(s) 34, 102
SchI GAGTC 1 cut(s) 735
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 4 cut(s) 241, 437, 547, 906
SfcI CTRYAG 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 972, 1009
SmoI CTYRAG 2 cut(s) 972, 1009
SpeI ACTAGT 2 cut(s) 29, 35
Sse9I AATT 7 cut(s) 184, 318, 664, 816, 952, 996, 1071
SsiI CCGC 5 cut(s) 42, 83, 248, 254, 416
SspMI CTAG 4 cut(s) 30, 36, 96, 650
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 4 cut(s) 124, 344, 586, 682
TaiI ACGT 2 cut(s) 508, 517
TaqI TCGA 3 cut(s) 188, 445, 729
TasI AATT 7 cut(s) 184, 318, 664, 816, 952, 996, 1071
TatI WGTACW 2 cut(s) 32, 100
TfiI GAWTC 2 cut(s) 170, 520
Tru1I TTAA 5 cut(s) 195, 588, 714, 848, 881
Tru9I TTAA 5 cut(s) 195, 588, 714, 848, 881
TscAI CASTG 2 cut(s) 127, 687
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 6 cut(s) 381, 384, 450, 806, 1039, 1062
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 66, 159, 485, 899
TspRI CASTG 2 cut(s) 127, 687
XapI RAATTY 1 cut(s) 664
XceI RCATGY 1 cut(s) 428
XspI CTAG 4 cut(s) 30, 36, 96, 650
ZrmI AGTACT 2 cut(s) 34, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.