MD03G1016900.v1.1

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000940
Physical Location & Seq
Reverse (-)
1331151 .. 1333526
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1016900.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGCCGAAGTTAGCGAAGCGCGAACGGAGCGCGAGTCAAACCCGAGGCGTTGACGAGGCCGCGCAGCTCAGAGCCGTGAAGCGCGAGAAGGTCAACGCCGGCGACCGAGTCGATGACGAGTCGGCGATCCCGAGTGAGCAGGACGCAGTTAGGCGGCGGGCACTCCGGTCCGACTACCTCGCCGTCAAGAACCTCATCAGTGAGAAAAGAGATGATTTGATGTGTCCGGATTCGGAGAAGTTCGGACTTCTCATCAATAAAGTCGAGAAATTGCATGAGCAAGTTCAGAAGCCAAGGGAACAAGTAGCAGACGCAGAGGCACTGCTGGACATAACAAACACATTGTGGACCTCTGTTAAGTCACAGTCCAGTGGCGGGATTAGCCCGTCGGACTTTGTTACTGCTTTGCTTTATAATTTTAGCCAACCGAGAGGAGGGGATGACCGTGTCTCGGTTAATTGGAAGGGAATTGGGTTTGCTGTTTTGCCCATTTTTAAGAGCGCTCATGGTTGTTGCACTGTGCTTGGACCCATGAGTACCGAGTTGAAGCAAAGGAAGGCTGTGGTTCGCAGTAAACGTGTGAAGCCTACTACAACTGATCGGCCTGATGAGATAGATGATACTCAGACGGAAGAGAAAACTGATACTGACAAGAACATGTCAACGATATTTGACATTCTAAGAAGGAAAAAGCGGGTTCGACTTGAAGCTTTGATATTAAACAGAAATTCTTTTGCGCAGACGGTGGAGAACTTATTTGCTCTGTCATTCTTAGTCAAAGATGGCCGAGCTGAATTATCCGTAGATGCAAATGGTTTTCATATTGTTTCACCTAGGAATGCTCCTTCCGCCGAGGACGTGGCATCACGGAAGGTCACTTACCACCATTTTGTGTTCCGGTTTGATTTCAAGGACTGGAAGGTAATGATGGATAGTTTGCCTGTTGGTGAGGAGCTGATGCCTCATAGATCCCCCCCAAACTCTACACGTACCTCTCAAGAGGAACCAGCAGTATATTTACCCACAACCCCGATTCGAAAACTCTCCAGAAACCGTGGCCGGGTTGTGCAGGAACAATCTATTGTAGAAGAATCCCCTGAAAACGATGACGCTATTGGAACTAACGTCTTTTGCAGGTCTAAGCACAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

385

Amino Acids

43.31

Weight (kDa)

9.01

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 233 - 320 5.9e-25 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc16I TGCGCA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 1125
AccII CGCG 4 cut(s) 21, 32, 62, 84
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 6 cut(s) 60, 154, 157, 375, 694, 849
AclWI GGATC 2 cut(s) 121, 963
AcoI YGGCCR 2 cut(s) 784, 1057
AcsI RAATTY 1 cut(s) 727
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 2 cut(s) 538, 991
AfeI AGCGCT 1 cut(s) 502
AfiI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1060
AflIII ACRYGT 3 cut(s) 577, 657, 986
AgsI TTSAA 3 cut(s) 547, 707, 910
AjiI CACGTC 1 cut(s) 859
AluBI AGCT 5 cut(s) 67, 710, 791, 955, 1150
AluI AGCT 5 cut(s) 67, 710, 791, 955, 1150
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 2 cut(s) 121, 963
Ama87I CYCGRG 2 cut(s) 42, 130
Aor13HI TCCGGA 1 cut(s) 226
Aor51HI AGCGCT 1 cut(s) 502
AoxI GGCC 4 cut(s) 57, 602, 784, 1057
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 727
AspA2I CCTAGG 1 cut(s) 833
AspLEI GCGC 6 cut(s) 21, 32, 64, 84, 503, 739
AspS9I GGNCC 3 cut(s) 168, 348, 527
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 2 cut(s) 822, 959
AsuII TTCGAA 1 cut(s) 1036
AvaI CYCGRG 2 cut(s) 42, 130
AvaII GGWCC 3 cut(s) 168, 348, 527
AvrII CCTAGG 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 776, 922
BceAI ACGGC 2 cut(s) 59, 167
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 1 cut(s) 834
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 1125
BisI GCNGC 3 cut(s) 60, 65, 155
BlnI CCTAGG 1 cut(s) 833
BlsI GCNGC 3 cut(s) 61, 66, 156
Bme1390I CCNGG 1 cut(s) 1061
Bme18I GGWCC 3 cut(s) 168, 348, 527
BmeT110I CYCGRG 2 cut(s) 42, 130
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 3 cut(s) 168, 348, 527
BmiI GGNNCC 2 cut(s) 529, 1005
BmrFI CCNGG 1 cut(s) 1061
BmsI GCATC 3 cut(s) 796, 872, 948
BpmI CTGGAG 1 cut(s) 1030
Bpu14I TTCGAA 1 cut(s) 1036
BpuEI CTTGAG 1 cut(s) 981
BpuMI CCSGG 1 cut(s) 1061
BsaAI YACGTR 1 cut(s) 989
BsaJI CCNNGG 5 cut(s) 43, 293, 833, 852, 1054
BsaWI WCCGGW 3 cut(s) 165, 226, 897
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1060
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 369, 920
BseAI TCCGGA 1 cut(s) 226
BseDI CCNNGG 5 cut(s) 43, 293, 833, 852, 1054
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1060
BseMII CTCAG 2 cut(s) 82, 638
BseNI ACTGG 2 cut(s) 369, 920
BseRI GAGGAG 2 cut(s) 447, 965
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 1088
Bsh1236I CGCG 4 cut(s) 21, 32, 62, 84
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 59, 604, 786, 1059
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 2 cut(s) 42, 130
BsiSI CCGG 5 cut(s) 99, 166, 227, 898, 1060
BslI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1060
BsmAI GTCTC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 4 cut(s) 59, 604, 786, 1059
BsoBI CYCGRG 2 cut(s) 42, 130
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 126, 598, 968
BspACI CCGC 6 cut(s) 60, 154, 157, 375, 694, 849
BspANI GGCC 4 cut(s) 59, 604, 786, 1059
BspCNI CTCAG 2 cut(s) 81, 637
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 4 cut(s) 21, 32, 62, 84
BspLI GGNNCC 2 cut(s) 529, 1005
BspMI ACCTGC 1 cut(s) 1125
BspPI GGATC 2 cut(s) 121, 963
BspT104I TTCGAA 1 cut(s) 1036
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 2 cut(s) 369, 920
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 5 cut(s) 43, 293, 833, 852, 1054
BssMI GATC 3 cut(s) 126, 598, 968
BssT1I CCWWGG 2 cut(s) 293, 833
Bst4CI ACNGT 5 cut(s) 366, 446, 520, 745, 1055
Bst6I CTCTTC 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 989
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 2 cut(s) 100, 159
BstDEI CTNAG 5 cut(s) 68, 624, 680, 772, 1140
BstDSI CCRYGG 1 cut(s) 1054
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 4 cut(s) 21, 32, 62, 84
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 6 cut(s) 21, 32, 64, 84, 503, 739
BstKTI GATC 3 cut(s) 129, 601, 971
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 3 cut(s) 126, 598, 968
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 27, 381, 848
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 1059
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 4 cut(s) 21, 32, 62, 84
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 968
BstYI RGATCY 1 cut(s) 968
BsuRI GGCC 4 cut(s) 59, 604, 786, 1059
BtgI CCRYGG 1 cut(s) 1054
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 1 cut(s) 445
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 4 cut(s) 205, 320, 376, 516
BveI ACCTGC 1 cut(s) 1125
Cac8I GCNNGC 2 cut(s) 100, 159
CfoI GCGC 6 cut(s) 21, 32, 64, 84, 503, 739
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 168, 348, 527
CpoI CGGWCCG 1 cut(s) 168
CseI GACGC 3 cut(s) 152, 320, 1118
Csp6I GTAC 2 cut(s) 537, 990
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 4 cut(s) 275, 506, 532, 658
CviQI GTAC 2 cut(s) 537, 990
DdeI CTNAG 5 cut(s) 68, 624, 680, 772, 1140
DpnI GATC 3 cut(s) 128, 600, 970
DpnII GATC 3 cut(s) 126, 598, 968
DraIII CACNNNGTG 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 784, 1057
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 838
Eco130I CCWWGG 2 cut(s) 293, 833
Eco47I GGWCC 3 cut(s) 168, 348, 527
Eco47III AGCGCT 1 cut(s) 502
Eco88I CYCGRG 2 cut(s) 42, 130
EcoT14I CCWWGG 2 cut(s) 293, 833
ErhI CCWWGG 2 cut(s) 293, 833
FaeI CATG 4 cut(s) 278, 509, 535, 661
FaiI YATR 9 cut(s) 276, 332, 414, 507, 533, 659, 822, 966, 1015
FatI CATG 4 cut(s) 274, 505, 531, 657
FauI CCCGC 3 cut(s) 150, 368, 687
Fnu4HI GCNGC 3 cut(s) 60, 65, 155
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 60, 65, 155
FspBI CTAG 1 cut(s) 834
FspI TGCGCA 1 cut(s) 738
GlaI GCGC 6 cut(s) 20, 31, 63, 83, 502, 738
GluI GCNGC 3 cut(s) 60, 65, 155
GsuI CTGGAG 1 cut(s) 1030
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 4 cut(s) 59, 604, 786, 1059
HapII CCGG 5 cut(s) 99, 166, 227, 898, 1060
HgaI GACGC 3 cut(s) 152, 320, 1118
HhaI GCGC 6 cut(s) 21, 32, 64, 84, 503, 739
Hin1II CATG 4 cut(s) 278, 509, 535, 661
Hin6I GCGC 6 cut(s) 19, 30, 62, 82, 501, 737
HinP1I GCGC 6 cut(s) 19, 30, 62, 82, 501, 737
HincII GTYRAC 3 cut(s) 52, 94, 663
HindII GTYRAC 3 cut(s) 52, 94, 663
HindIII AAGCTT 2 cut(s) 708, 1148
HinfI GANTC 6 cut(s) 34, 108, 119, 230, 1033, 1091
HpaII CCGG 5 cut(s) 99, 166, 227, 898, 1060
HphI GGTGA 2 cut(s) 822, 959
Hpy166II GTNNAC 5 cut(s) 52, 94, 348, 575, 663
Hpy188I TCNGA 7 cut(s) 71, 172, 235, 245, 288, 391, 627
Hpy188III TCNNGA 6 cut(s) 130, 187, 227, 265, 998, 1047
Hpy8I GTNNAC 5 cut(s) 52, 94, 348, 575, 663
Hpy99I CGWCG 1 cut(s) 391
HpyAV CCTTC 7 cut(s) 82, 457, 550, 678, 855, 865, 913
HpyCH4III ACNGT 5 cut(s) 366, 446, 520, 745, 1055
HpyCH4IV ACGT 4 cut(s) 577, 858, 988, 1125
HpyCH4V TGCA 5 cut(s) 274, 516, 809, 1069, 1134
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 381, 848
HpyF3I CTNAG 5 cut(s) 68, 624, 680, 772, 1140
HpySE526I ACGT 4 cut(s) 577, 858, 988, 1125
Hsp92II CATG 4 cut(s) 278, 509, 535, 661
HspAI GCGC 6 cut(s) 19, 30, 62, 82, 501, 737
Kpn2I TCCGGA 1 cut(s) 226
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 3 cut(s) 126, 598, 968
LmnI GCTCC 3 cut(s) 27, 847, 952
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 3 cut(s) 796, 872, 948
MaeI CTAG 1 cut(s) 834
MaeII ACGT 4 cut(s) 577, 858, 988, 1125
MaeIII GTNAC 3 cut(s) 360, 397, 874
MalI GATC 3 cut(s) 128, 600, 970
MboI GATC 3 cut(s) 126, 598, 968
MboII GAAGA 2 cut(s) 644, 1100
MflI RGATCY 1 cut(s) 968
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 6 cut(s) 269, 415, 457, 468, 727, 794
MlyI GAGTC 3 cut(s) 43, 117, 128
MmeI TCCRAC 2 cut(s) 195, 369
MreI CGCCGGCG 1 cut(s) 98
MroI TCCGGA 1 cut(s) 226
MroNI GCCGGC 1 cut(s) 98
MseI TTAA 5 cut(s) 357, 456, 495, 719, 1153
MspI CCGG 5 cut(s) 99, 166, 227, 898, 1060
MspR9I CCNGG 1 cut(s) 1061
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 4 cut(s) 21, 32, 62, 84
MwoI GCNNNNNNNGC 3 cut(s) 27, 381, 848
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 1061
NdeII GATC 3 cut(s) 126, 598, 968
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 4 cut(s) 278, 509, 535, 661
NlaIV GGNNCC 2 cut(s) 529, 1005
NmeAIII GCCGAG 2 cut(s) 812, 877
NmuCI GTSAC 2 cut(s) 360, 874
NsbI TGCGCA 1 cut(s) 738
NspI RCATGY 1 cut(s) 661
NspV TTCGAA 1 cut(s) 1036
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 574
PctI GAATGC 1 cut(s) 844
PdiI GCCGGC 1 cut(s) 100
PfeI GAWTC 3 cut(s) 230, 1033, 1091
PflFI GACNNNGTC 2 cut(s) 107, 446
PkrI GCNGC 3 cut(s) 61, 66, 156
PleI GAGTC 3 cut(s) 42, 116, 127
PpsI GAGTC 3 cut(s) 42, 116, 127
Ppu21I YACGTR 1 cut(s) 989
PscI ACATGT 1 cut(s) 657
PsiI TTATAA 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 529, 1005
PspPI GGNCC 3 cut(s) 168, 348, 527
PsuI RGATCY 1 cut(s) 968
PsyI GACNNNGTC 2 cut(s) 107, 446
RsaI GTAC 2 cut(s) 538, 991
RsaNI GTAC 2 cut(s) 537, 990
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SaqAI TTAA 5 cut(s) 357, 456, 495, 719, 1153
SatI GCNGC 3 cut(s) 60, 65, 155
Sau3AI GATC 3 cut(s) 126, 598, 968
Sau96I GGNCC 3 cut(s) 168, 348, 527
SchI GAGTC 3 cut(s) 43, 117, 128
ScrFI CCNGG 1 cut(s) 1061
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 3 cut(s) 796, 872, 948
SfuI TTCGAA 1 cut(s) 1036
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 3 cut(s) 168, 348, 527
SmlI CTYRAG 1 cut(s) 996
SmoI CTYRAG 1 cut(s) 996
Sse9I AATT 6 cut(s) 269, 415, 457, 468, 727, 794
SsiI CCGC 6 cut(s) 60, 154, 157, 375, 694, 849
SspMI CTAG 1 cut(s) 834
StyD4I CCNGG 1 cut(s) 1059
StyI CCWWGG 2 cut(s) 293, 833
TaaI ACNGT 5 cut(s) 366, 446, 520, 745, 1055
TaiI ACGT 4 cut(s) 580, 861, 991, 1128
TaqI TCGA 4 cut(s) 111, 264, 700, 1036
TaqII GACCGA 1 cut(s) 120
TasI AATT 6 cut(s) 269, 415, 457, 468, 727, 794
TauI GCSGC 2 cut(s) 62, 157
TfiI GAWTC 3 cut(s) 230, 1033, 1091
Tru1I TTAA 5 cut(s) 357, 456, 495, 719, 1153
Tru9I TTAA 5 cut(s) 357, 456, 495, 719, 1153
TscAI CASTG 4 cut(s) 205, 327, 376, 523
TseFI GTSAC 2 cut(s) 360, 874
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 2 cut(s) 360, 874
TspDTI ATGAA 1 cut(s) 809
TspGWI ACGGA 4 cut(s) 40, 644, 790, 883
TspRI CASTG 4 cut(s) 205, 327, 376, 523
Tth111I GACNNNGTC 2 cut(s) 107, 446
VpaK11BI GGWCC 3 cut(s) 168, 348, 527
XapI RAATTY 1 cut(s) 727
XceI RCATGY 1 cut(s) 661
XmaJI CCTAGG 1 cut(s) 833
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.