FvH4_3g44150

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000940
Physical Location & Seq
Forward (+)
36808788 .. 36811431
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44150.t1

Sequence Viewer

Length: 1011 bp
ATGGCTGAGAAAGAGTGTCATGTCAATCCTGAGCTACTCAACTTCGGCCAAGACCCGACTCCACGGCGAGAGCTCCGTTCCGAGTACCTCGCCGTCCGGAGCTTCACCAGTGAGAAAAAAGACGATTTAGTGAGCCCGGATTCGAAGAAGTTTGATGTGATCATTGACTACATTGACAAATTGCACGAGCAAGAAACTACCCCAACCGATTTCATTACCGGTTTGATTACACATTTCGGGCAAGTTAAGTCATCAGTGAACAAAGATGAGGCCAATGTGTCGCTTAAATGGAAAGACATTGGAATTTTTGTTTCACCAATACTCAAGAGGGCTCTTGGATGTTGCACAATGCTTGGACCCATGAATGCTGTGATCAAGCAAAAGAAGGCTGCTGTCTGTCGAACAAAGCCTACCACAGCTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCGAAAGAACTGATACAGACTTGAACATGGCAACAATGTTTGAGATTTTAAAGAACAAGAAGAAAGTTGATCTTAATTGTTTGATACTGAATAGAAGGTCTTTTGCACAGACAGTGGAGAATCTTTTTGCTCTGTCATTTCTAGCGAAAGATGGCAGAGTTAGAATTGCTGTCCAAGTAAATGGTTCTCATATTGTTTCACCTACGAATGGTCCTGTTGCCAATGAAGTGGCCTATCACCATTTTGTGTTTCGATTTGATTTCAAGGAATGGAAGATCATGATAGAAATGGTGGCTGTTGGTGAAGAGCTCATGCCTCATAGAAACCACACAAAGTGCACACCTGCCTCGGGTGACAAACCAGCAGCTTTCAATTCCCAAACAGACACCCCACACACAATCCAGACTCGAAAACATACCAGAAACGATGGCCTGGTTGTACAGGATAAATCTACTGCAGAAGAATTCTTGGAAAATGATGATGCTGCTGGAGCTACAGCACTACGCAGGTGTAAGCGCAAGCTTTTGTTTAATTCATGTGTCAGCGGAAGTTTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

337

Amino Acids

37.69

Weight (kDa)

8.53

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 173 - 255 2.8e-20 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 802, 948
Acc36I ACCTGC 2 cut(s) 802, 948
AccIII TCCGGA 1 cut(s) 96
AciI CCGC 1 cut(s) 996
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 303, 914
AdeI CACNNNGTG 1 cut(s) 786
AfaI GTAC 2 cut(s) 86, 891
AfiI CCNNNNNNNGG 2 cut(s) 659, 800
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 475, 715, 823
AjnI CCWGG 1 cut(s) 882
AluBI AGCT 8 cut(s) 34, 73, 102, 419, 760, 818, 944, 973
AluI AGCT 8 cut(s) 34, 73, 102, 419, 760, 818, 944, 973
Alw21I GWGCWC 3 cut(s) 75, 762, 791
Alw44I GTGCAC 1 cut(s) 787
Ama87I CYCGRG 1 cut(s) 799
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 4 cut(s) 46, 270, 681, 880
ApaLI GTGCAC 1 cut(s) 787
ApeKI GCWGC 3 cut(s) 389, 815, 935
ApoI RAATTY 2 cut(s) 303, 914
AsiGI ACCGGT 1 cut(s) 218
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 2 cut(s) 356, 662
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 6 cut(s) 97, 306, 642, 680, 764, 815
AsuII TTCGAA 1 cut(s) 143
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 2 cut(s) 356, 662
BaeGI GKGCMC 1 cut(s) 791
BanII GRGCYC 4 cut(s) 75, 137, 334, 762
BauI CACGAG 1 cut(s) 185
Bbv12I GWGCWC 3 cut(s) 75, 762, 791
BbvI GCAGC 3 cut(s) 376, 827, 922
BccI CCATC 2 cut(s) 596, 872
BceAI ACGGC 2 cut(s) 77, 80
BciT130I CCWGG 1 cut(s) 884
BclI TGATCA 2 cut(s) 159, 372
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 2 cut(s) 906, 945
BfuAI ACCTGC 2 cut(s) 802, 948
BisI GCNGC 3 cut(s) 390, 816, 936
BlsI GCNGC 3 cut(s) 391, 817, 937
Bme1390I CCNGG 2 cut(s) 137, 884
Bme18I GGWCC 2 cut(s) 356, 662
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 2 cut(s) 356, 662
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 137, 884
BmsI GCATC 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 960
Bpu10I CCTNAGC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 62, 429, 798
BsaWI WCCGGW 2 cut(s) 96, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 659, 800
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 1 cut(s) 884
BseDI CCNNGG 3 cut(s) 62, 429, 798
BseGI GGATG 1 cut(s) 344
BseLI CCNNNNNNNGG 2 cut(s) 659, 800
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 791
BseXI GCAGC 3 cut(s) 376, 827, 922
BshFI GGCC 4 cut(s) 48, 272, 683, 882
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 3 cut(s) 75, 762, 791
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 3 cut(s) 97, 137, 219
BslI CCNNNNNNNGG 2 cut(s) 659, 800
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 4 cut(s) 48, 272, 683, 882
BsoBI CYCGRG 1 cut(s) 799
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 5 cut(s) 75, 137, 334, 762, 791
Bsp13I TCCGGA 1 cut(s) 96
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 5 cut(s) 159, 372, 435, 520, 726
BspACI CCGC 1 cut(s) 996
BspANI GGCC 4 cut(s) 48, 272, 683, 882
BspCNI CTCAG 1 cut(s) 22
BspEI TCCGGA 1 cut(s) 96
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 1 cut(s) 358
BspMAI CTGCAG 1 cut(s) 910
BspMI ACCTGC 2 cut(s) 802, 948
BspQI GCTCTTC 1 cut(s) 750
BspT104I TTCGAA 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 3 cut(s) 62, 429, 798
BssMI GATC 5 cut(s) 159, 372, 435, 520, 726
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 1 cut(s) 565
Bst6I CTCTTC 1 cut(s) 750
BstAUI TGTACA 1 cut(s) 889
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 971
BstDEI CTNAG 2 cut(s) 6, 30
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 5 cut(s) 162, 375, 438, 523, 729
BstMBI GATC 5 cut(s) 159, 372, 435, 520, 726
BstMWI GCNNNNNNNGC 1 cut(s) 941
BstNI CCWGG 1 cut(s) 884
BstSCI CCNGG 2 cut(s) 135, 882
BstSFI CTRYAG 2 cut(s) 906, 945
BstSLI GKGCMC 1 cut(s) 791
BstV1I GCAGC 3 cut(s) 376, 827, 922
BstXI CCANNNNNNTGG 2 cut(s) 632, 679
BsuRI GGCC 4 cut(s) 48, 272, 683, 882
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 1 cut(s) 344
BtsIMutI CAGTG 3 cut(s) 115, 261, 570
BveI ACCTGC 2 cut(s) 802, 948
Cac8I GCNNGC 1 cut(s) 971
CciI TCATGA 1 cut(s) 729
CfoI GCGC 1 cut(s) 969
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 356, 662
Csp6I GTAC 2 cut(s) 85, 890
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 6 cut(s) 20, 361, 478, 730, 763, 987
CviQI GTAC 2 cut(s) 85, 890
DdeI CTNAG 2 cut(s) 6, 30
DpnI GATC 5 cut(s) 161, 374, 437, 522, 728
DpnII GATC 5 cut(s) 159, 372, 435, 520, 726
DraI TTTAAA 1 cut(s) 501
DraIII CACNNNGTG 1 cut(s) 786
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
Ecl136II GAGCTC 2 cut(s) 73, 760
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 4 cut(s) 75, 137, 334, 762
Eco47I GGWCC 2 cut(s) 356, 662
Eco53kI GAGCTC 2 cut(s) 73, 760
Eco88I CYCGRG 1 cut(s) 799
EcoICRI GAGCTC 2 cut(s) 73, 760
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 882
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 4 cut(s) 75, 137, 334, 762
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 6 cut(s) 23, 364, 481, 733, 766, 990
FaiI YATR 9 cut(s) 21, 362, 479, 642, 731, 764, 771, 867, 988
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FatI CATG 6 cut(s) 19, 360, 477, 729, 762, 986
FbaI TGATCA 2 cut(s) 159, 372
Fnu4HI GCNGC 3 cut(s) 390, 816, 936
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 4 cut(s) 75, 137, 334, 762
Fsp4HI GCNGC 3 cut(s) 390, 816, 936
FspBI CTAG 1 cut(s) 593
GlaI GCGC 1 cut(s) 968
GluI GCNGC 3 cut(s) 390, 816, 936
GsuI CTGGAG 1 cut(s) 960
HaeIII GGCC 4 cut(s) 48, 272, 683, 882
HapII CCGG 3 cut(s) 97, 137, 219
HhaI GCGC 1 cut(s) 969
Hin1II CATG 6 cut(s) 23, 364, 481, 733, 766, 990
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 4 cut(s) 58, 140, 571, 856
HpaII CCGG 3 cut(s) 97, 137, 219
HphI GGTGA 6 cut(s) 97, 306, 642, 680, 764, 815
Hpy166II GTNNAC 2 cut(s) 259, 789
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 5 cut(s) 29, 97, 325, 730, 853
Hpy8I GTNNAC 2 cut(s) 259, 789
HpyAV CCTTC 3 cut(s) 379, 440, 540
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4V TGCA 5 cut(s) 184, 345, 557, 789, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 941
HpyF3I CTNAG 2 cut(s) 6, 30
Hsp92II CATG 6 cut(s) 23, 364, 481, 733, 766, 990
HspAI GCGC 1 cut(s) 967
Kpn2I TCCGGA 1 cut(s) 96
Ksp22I TGATCA 2 cut(s) 159, 372
Kzo9I GATC 5 cut(s) 159, 372, 435, 520, 726
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 3 cut(s) 78, 99, 941
Lsp1109I GCAGC 3 cut(s) 376, 827, 922
LweI GCATC 1 cut(s) 922
MaeI CTAG 1 cut(s) 593
MaeIII GTNAC 1 cut(s) 803
MalI GATC 5 cut(s) 161, 374, 437, 522, 728
MboI GATC 5 cut(s) 159, 372, 435, 520, 726
MboII GAAGA 5 cut(s) 157, 523, 736, 767, 923
MhlI GDGCHC 5 cut(s) 75, 137, 334, 762, 791
MluCI AATT 7 cut(s) 179, 303, 526, 615, 823, 914, 982
MlyI GAGTC 2 cut(s) 52, 850
MnlI CCTC 6 cut(s) 98, 262, 321, 443, 777, 808
MroI TCCGGA 1 cut(s) 96
MseI TTAA 6 cut(s) 246, 285, 500, 525, 981, 1004
MspA1I CMGCKG 1 cut(s) 996
MspI CCGG 3 cut(s) 97, 137, 219
MspR9I CCNGG 2 cut(s) 137, 884
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 884
MwoI GCNNNNNNNGC 1 cut(s) 941
NciI CCSGG 1 cut(s) 137
NdeII GATC 5 cut(s) 159, 372, 435, 520, 726
NlaIII CATG 6 cut(s) 23, 364, 481, 733, 766, 990
NlaIV GGNNCC 1 cut(s) 358
NmuCI GTSAC 1 cut(s) 803
NspV TTCGAA 1 cut(s) 143
PagI TCATGA 1 cut(s) 729
PaqCI CACCTGC 2 cut(s) 802, 948
PciSI GCTCTTC 1 cut(s) 750
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 2 cut(s) 140, 571
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 3 cut(s) 391, 817, 937
PleI GAGTC 2 cut(s) 52, 850
PpsI GAGTC 2 cut(s) 52, 850
Psp124BI GAGCTC 2 cut(s) 75, 762
Psp6I CCWGG 1 cut(s) 882
PspGI CCWGG 1 cut(s) 882
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 2 cut(s) 356, 662
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
PstI CTGCAG 1 cut(s) 910
RsaI GTAC 2 cut(s) 86, 891
RsaNI GTAC 2 cut(s) 85, 890
SacI GAGCTC 2 cut(s) 75, 762
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 6 cut(s) 246, 285, 500, 525, 981, 1004
SatI GCNGC 3 cut(s) 390, 816, 936
Sau3AI GATC 5 cut(s) 159, 372, 435, 520, 726
Sau96I GGNCC 2 cut(s) 356, 662
SchI GAGTC 2 cut(s) 52, 850
ScrFI CCNGG 2 cut(s) 137, 884
SduI GDGCHC 5 cut(s) 75, 137, 334, 762, 791
SfaNI GCATC 1 cut(s) 922
SfcI CTRYAG 2 cut(s) 906, 945
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 2 cut(s) 356, 662
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 7 cut(s) 179, 303, 526, 615, 823, 914, 982
SsiI CCGC 1 cut(s) 996
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 2 cut(s) 75, 762
StyD4I CCNGG 2 cut(s) 135, 882
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 1 cut(s) 565
TaqI TCGA 4 cut(s) 143, 400, 703, 859
TasI AATT 7 cut(s) 179, 303, 526, 615, 823, 914, 982
TatI WGTACW 1 cut(s) 889
TfiI GAWTC 2 cut(s) 140, 571
Tru1I TTAA 6 cut(s) 246, 285, 500, 525, 981, 1004
Tru9I TTAA 6 cut(s) 246, 285, 500, 525, 981, 1004
TscAI CASTG 3 cut(s) 115, 261, 570
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 3 cut(s) 389, 815, 935
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 5 cut(s) 202, 377, 459, 690, 975
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 3 cut(s) 115, 261, 570
VneI GTGCAC 1 cut(s) 787
VpaK11BI GGWCC 2 cut(s) 356, 662
XapI RAATTY 2 cut(s) 303, 914
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.