Prupe.7G076100_v2.0.a1

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000940
Physical Location & Seq
Forward (+)
11293214 .. 11297499
4286 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G076100.1

Sequence Viewer

Length: 1149 bp
ATGATGGAACGTGAATTGGCCGGTAGCAGTAGCCGTGGAAGAGATAGAATGGAGCAGCCGCAAGCCGTGGCTGACCGTAGAGCTCTCCGATCACGCTATTTTACTGTGAAGACCCTCATTCACGATGAAAGAGATGACATTACAAAAGTGGATTCCGACAAGTTTAATTCGATCATTAATGAAGTGGATAGCTTGCATGAGATGGTACAAAATCCAAGAGAGCAAGTTGCTGATGCAGAGGCGCTTTTGGACATTGCAAACACCTTGATGACCTCTGTGAAGGCGCAGAACAAGGAAGGAATCACAGCTACAGATTTTGTCTCCTGTATTCTTAGAGACTTTGGGCAACAAAGTGGGCTGAGAAGTAACAGAGAAGGGGTTAGCTGCTCAATATCTTGGAAGGATATTGGGACTGAAGTCTCAAATGTTTTCCAGAGAAGCCCCCAATGCTGCACTATGATTGGGCCTATGAATGCTGAAGTAAAGCAACGGAAGGCTCATGTTCATACGAAACGTGTGAAGCCTACAGAAAATGATACCCCAGAAGAGCTTGATAATGTTGCTGTAGAAGAGGAACCTGAGACGGTTAAGAATGTGGCAGCAATGTTTAATATCTTGAGGAAAAACAGAAGAGCCAGGCTTGAAAATCTAGTCCTGAATGGGAATTCTTTTGCGCAGACGGTGGAAAATTTATTTGCCCTCTCATTTCTGGTTAAAGATGGGCGGGCTGAAATAAAAATTAATGAAGAAGGCCATCATCTAGTTTCTCCAAGGAATGCTCCTGCTGCCAAAGCAATTGCCTCAGGGGAGGTTGCTTACTGCCATTTCGTGTTCAGATTTGATTTCAAGGACTGGAAGTTGATGAAAGAGTCTGTTGGGGATGGAGAGCAGCTGATGCCACACCGGCGTCAAGAAAACATATCAAGCAATTCTCAATTTGATCCGCAATGTGTAGAATCTGAAGCAACCACAGCTAAAACACCAATTAGGAAGTTATCCAGAAACAGAGGCTTGGTTTTACAGCTGCGGCCAGTTGGGGGATGCGGCACGGCTCATAGTAATCAATCAGTTGTAGAAGACTCGCCGGTATGTGATTACTCTGAAGATAGAACTGCTGCCATCCGAAAAGGGAAGCGTAAACTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

383

Amino Acids

42.9

Weight (kDa)

7.12

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 675
AciI CCGC 5 cut(s) 59, 724, 944, 1027, 1044
AclWI GGATC 1 cut(s) 935
AcoI YGGCCR 2 cut(s) 18, 1028
AcsI RAATTY 2 cut(s) 664, 688
AcuI CTGAAG 4 cut(s) 435, 498, 981, 1122
AcyI GRCGYC 1 cut(s) 907
AfaI GTAC 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 1037
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 644, 847
AjnI CCWGG 1 cut(s) 635
AluBI AGCT 8 cut(s) 83, 192, 308, 384, 550, 892, 974, 1024
AluI AGCT 8 cut(s) 83, 192, 308, 384, 550, 892, 974, 1024
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 4 cut(s) 325, 330, 424, 575
AlwI GGATC 1 cut(s) 935
AoxI GGCC 4 cut(s) 18, 464, 751, 1028
ApeKI GCWGC 8 cut(s) 55, 384, 450, 599, 785, 889, 1024, 1115
ApoI RAATTY 2 cut(s) 664, 688
AseI ATTAAT 2 cut(s) 177, 741
AspLEI GCGC 3 cut(s) 244, 286, 676
AspS9I GGNCC 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 802
BanII GRGCYC 1 cut(s) 85
BbsI GAAGAC 2 cut(s) 116, 1083
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 8 cut(s) 67, 371, 437, 611, 772, 901, 1011, 1102
BccI CCATC 5 cut(s) 196, 713, 762, 875, 1127
BceAI ACGGC 3 cut(s) 18, 50, 1065
BciT130I CCWGG 1 cut(s) 637
BcoDI GTCTC 4 cut(s) 325, 330, 424, 575
BfaI CTAG 2 cut(s) 650, 761
BfmI CTRYAG 3 cut(s) 309, 525, 564
BfoI RGCGCY 1 cut(s) 245
BglI GCCNNNNNGGC 1 cut(s) 904
Bme1390I CCNGG 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 1 cut(s) 637
BmsI GCATC 3 cut(s) 223, 885, 1031
BoxI GACNNNNGTC 1 cut(s) 416
BpiI GAAGAC 2 cut(s) 116, 1083
BpuEI CTTGAG 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 907
BsaJI CCNNGG 3 cut(s) 34, 66, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 1037
Bse118I RCCGGY 3 cut(s) 20, 903, 1084
Bse1I ACTGG 2 cut(s) 857, 1031
Bse21I CCTNAGG 1 cut(s) 802
Bse3DI GCAATG 3 cut(s) 252, 609, 953
BseBI CCWGG 1 cut(s) 637
BseDI CCNNGG 3 cut(s) 34, 66, 770
BseGI GGATG 3 cut(s) 886, 1046, 1119
BseLI CCNNNNNNNGG 1 cut(s) 1037
BseMI GCAATG 3 cut(s) 252, 609, 953
BseMII CTCAG 3 cut(s) 350, 570, 816
BseNI ACTGG 2 cut(s) 857, 1031
BseXI GCAGC 8 cut(s) 67, 371, 437, 611, 772, 901, 1011, 1102
BsgI GTGCAG 1 cut(s) 436
BshFI GGCC 4 cut(s) 20, 466, 753, 1030
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 3 cut(s) 21, 904, 1085
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 1 cut(s) 1037
BsmAI GTCTC 4 cut(s) 325, 330, 424, 575
BsmBI CGTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 424
BsmI GAATGC 2 cut(s) 478, 781
BsnI GGCC 4 cut(s) 20, 466, 753, 1030
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 3 cut(s) 89, 171, 940
BspACI CCGC 5 cut(s) 59, 724, 944, 1027, 1044
BspANI GGCC 4 cut(s) 20, 466, 753, 1030
BspCNI CTCAG 3 cut(s) 351, 571, 815
BspLI GGNNCC 1 cut(s) 576
BspPI GGATC 1 cut(s) 935
BspQI GCTCTTC 2 cut(s) 540, 625
BsrDI GCAATG 3 cut(s) 252, 609, 953
BsrFI RCCGGY 3 cut(s) 20, 903, 1084
BsrI ACTGG 2 cut(s) 857, 1031
BssAI RCCGGY 3 cut(s) 20, 903, 1084
BssECI CCNNGG 3 cut(s) 34, 66, 770
BssMI GATC 3 cut(s) 89, 171, 940
BssNI GRCGYC 1 cut(s) 907
BssT1I CCWWGG 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 637
Bst4CI ACNGT 4 cut(s) 77, 106, 586, 682
Bst6I CTCTTC 4 cut(s) 34, 540, 564, 625
BstACI GRCGYC 1 cut(s) 907
BstAPI GCANNNNNTGC 1 cut(s) 895
BstC8I GCNNGC 3 cut(s) 63, 194, 726
BstDEI CTNAG 4 cut(s) 332, 359, 579, 802
BstDSI CCRYGG 2 cut(s) 34, 66
BstF5I GGATG 3 cut(s) 886, 1046, 1119
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 3 cut(s) 244, 286, 676
BstKTI GATC 3 cut(s) 92, 174, 943
BstMAI GTCTC 4 cut(s) 325, 330, 424, 575
BstMBI GATC 3 cut(s) 89, 171, 940
BstMWI GCNNNNNNNGC 6 cut(s) 447, 785, 791, 895, 904, 971
BstNI CCWGG 1 cut(s) 637
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 635
BstSFI CTRYAG 3 cut(s) 309, 525, 564
BstV1I GCAGC 8 cut(s) 67, 371, 437, 611, 772, 901, 1011, 1102
BstV2I GAAGAC 2 cut(s) 116, 1083
Bsu36I CCTNAGG 1 cut(s) 802
BsuRI GGCC 4 cut(s) 20, 466, 753, 1030
BtgI CCRYGG 2 cut(s) 34, 66
BtsCI GGATG 3 cut(s) 886, 1046, 1119
Cac8I GCNNGC 3 cut(s) 63, 194, 726
CfoI GCGC 3 cut(s) 244, 286, 676
Cfr10I RCCGGY 3 cut(s) 20, 903, 1084
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 896
Csp6I GTAC 1 cut(s) 206
CviAII CATG 2 cut(s) 197, 500
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 4 cut(s) 332, 359, 579, 802
DpnI GATC 3 cut(s) 91, 173, 942
DpnII GATC 3 cut(s) 89, 171, 940
EaeI YGGCCR 2 cut(s) 18, 1028
Eam1104I CTCTTC 4 cut(s) 34, 540, 564, 625
EarI CTCTTC 4 cut(s) 34, 540, 564, 625
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 4 cut(s) 435, 498, 981, 1122
Eco81I CCTNAGG 1 cut(s) 802
EcoICRI GAGCTC 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 635
EcoT14I CCWWGG 1 cut(s) 770
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 770
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 2 cut(s) 200, 503
FaiI YATR 8 cut(s) 198, 458, 470, 501, 507, 920, 1056, 1090
FaqI GGGAC 1 cut(s) 424
FatI CATG 2 cut(s) 196, 499
FauI CCCGC 1 cut(s) 717
FokI GGATG 3 cut(s) 893, 1053, 1106
FriOI GRGCYC 1 cut(s) 85
FspBI CTAG 2 cut(s) 650, 761
FspI TGCGCA 1 cut(s) 675
GlaI GCGC 3 cut(s) 243, 285, 675
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 4 cut(s) 20, 466, 753, 1030
HapII CCGG 3 cut(s) 21, 904, 1085
HgaI GACGC 1 cut(s) 896
HhaI GCGC 3 cut(s) 244, 286, 676
Hin1I GRCGYC 1 cut(s) 907
Hin1II CATG 2 cut(s) 200, 503
Hin6I GCGC 3 cut(s) 242, 284, 674
HinP1I GCGC 3 cut(s) 242, 284, 674
HinfI GANTC 5 cut(s) 152, 300, 869, 956, 1079
HpaII CCGG 3 cut(s) 21, 904, 1085
Hpy166II GTNNAC 1 cut(s) 1139
Hpy188I TCNGA 6 cut(s) 89, 157, 836, 961, 1102, 1124
Hpy188III TCNNGA 6 cut(s) 122, 433, 616, 655, 911, 999
Hpy8I GTNNAC 1 cut(s) 1139
HpyAV CCTTC 6 cut(s) 274, 290, 368, 394, 487, 743
HpyCH4III ACNGT 4 cut(s) 77, 106, 586, 682
HpyCH4IV ACGT 2 cut(s) 10, 514
HpyCH4V TGCA 4 cut(s) 196, 236, 257, 453
HpyF10VI GCNNNNNNNGC 6 cut(s) 447, 785, 791, 895, 904, 971
HpyF3I CTNAG 4 cut(s) 332, 359, 579, 802
HpySE526I ACGT 2 cut(s) 10, 514
Hsp92I GRCGYC 1 cut(s) 907
Hsp92II CATG 2 cut(s) 200, 503
HspAI GCGC 3 cut(s) 242, 284, 674
Kzo9I GATC 3 cut(s) 89, 171, 940
LguI GCTCTTC 2 cut(s) 540, 625
LmnI GCTCC 2 cut(s) 52, 784
Lsp1109I GCAGC 8 cut(s) 67, 371, 437, 611, 772, 901, 1011, 1102
LweI GCATC 3 cut(s) 223, 885, 1031
MaeI CTAG 2 cut(s) 650, 761
MaeII ACGT 2 cut(s) 10, 514
MaeIII GTNAC 1 cut(s) 365
MalI GATC 3 cut(s) 91, 173, 942
MboI GATC 3 cut(s) 89, 171, 940
MboII GAAGA 8 cut(s) 51, 121, 557, 581, 642, 758, 1088, 1115
MfeI CAATTG 1 cut(s) 795
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 9 cut(s) 14, 166, 664, 688, 738, 795, 928, 935, 984
MlyI GAGTC 2 cut(s) 878, 1073
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 9 cut(s) 125, 232, 283, 565, 612, 710, 802, 811, 1001
MseI TTAA 6 cut(s) 165, 177, 588, 609, 714, 741
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 892, 1024
MspI CCGG 3 cut(s) 21, 904, 1085
MspR9I CCNGG 1 cut(s) 637
MunI CAATTG 1 cut(s) 795
Mva1269I GAATGC 2 cut(s) 478, 781
MvaI CCWGG 1 cut(s) 637
MwoI GCNNNNNNNGC 6 cut(s) 447, 785, 791, 895, 904, 971
NdeII GATC 3 cut(s) 89, 171, 940
NlaIII CATG 2 cut(s) 200, 503
NlaIV GGNNCC 1 cut(s) 576
NsbI TGCGCA 1 cut(s) 675
PciSI GCTCTTC 2 cut(s) 540, 625
PctI GAATGC 2 cut(s) 478, 781
PfeI GAWTC 3 cut(s) 152, 300, 956
PleI GAGTC 2 cut(s) 877, 1073
PpsI GAGTC 2 cut(s) 877, 1073
PshAI GACNNNNGTC 1 cut(s) 416
PshBI ATTAAT 2 cut(s) 177, 741
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 635
PspGI CCWGG 1 cut(s) 635
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 1 cut(s) 464
PvuII CAGCTG 2 cut(s) 892, 1024
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 2 cut(s) 540, 625
SaqAI TTAA 6 cut(s) 165, 177, 588, 609, 714, 741
Sau3AI GATC 3 cut(s) 89, 171, 940
Sau96I GGNCC 1 cut(s) 464
SchI GAGTC 2 cut(s) 878, 1073
ScrFI CCNGG 1 cut(s) 637
SduI GDGCHC 1 cut(s) 85
SfaNI GCATC 3 cut(s) 223, 885, 1031
SfcI CTRYAG 3 cut(s) 309, 525, 564
SgrAI CRCCGGYG 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 9 cut(s) 14, 166, 664, 688, 738, 795, 928, 935, 984
SsiI CCGC 5 cut(s) 59, 724, 944, 1027, 1044
SspMI CTAG 2 cut(s) 650, 761
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 635
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 4 cut(s) 77, 106, 586, 682
TaiI ACGT 2 cut(s) 13, 517
TaqI TCGA 1 cut(s) 170
TasI AATT 9 cut(s) 14, 166, 664, 688, 738, 795, 928, 935, 984
TauI GCSGC 3 cut(s) 61, 1030, 1047
TfiI GAWTC 3 cut(s) 152, 300, 956
Tru1I TTAA 6 cut(s) 165, 177, 588, 609, 714, 741
Tru9I TTAA 6 cut(s) 165, 177, 588, 609, 714, 741
TseI GCWGC 8 cut(s) 55, 384, 450, 599, 785, 889, 1024, 1115
TspDTI ATGAA 6 cut(s) 141, 195, 485, 494, 759, 878
TspGWI ACGGA 1 cut(s) 505
VspI ATTAAT 2 cut(s) 177, 741
XapI RAATTY 2 cut(s) 664, 688
XspI CTAG 2 cut(s) 650, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.