pycom11g01240

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000940
Physical Location & Seq
Reverse (-)
983424 .. 985458
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g01240.2

Sequence Viewer

Length: 1170 bp
ATGCCGAAGTCAGCGAAGCGCGAACGGATCGCGAGTCAAACCCGAGGCGTTGACGATGCCGCGCAGCTCAGAGCCGTGAAGCGCGAGAAGGTCAACGCCGGCGACCGAGTGGATGACGAGTCGTCTCGCCCGAGTGAGCAGGACGCCGTTCGGCGGCGGGCGCTCCGGTCCGACTACCTCGCCGTCAAGAACCTCATCAGTGAGAAAAGAGATGATTTGATGTGCCCGGATTCGGAGAAGTTCGGACTTCTCATCAATGAAGTCGAGAAATTGCATGAGCAAGTTCAGAAGCCAAGGGAACAAGTAGCAGATGCAGAGGCACTGCTGGACATAACTAACACATTGTGGACCTCTGTTAAGTCACAGTCCAGTGCCGGGATTAGCCCGTCCGACTTTGTTACTGCTTTGCTTAATAATTTTAGCCAACCGAGAGGAGGGGATGACCATGTCTCAGTTAATTGGAAGGAAATTGGGTTGGCTGTTTTGCCCATTTTCAAGAATGCTCATGGGTGTTGCACTATGCTTGGACCCATGAGTACTGAGTTGAAGCAAAGGAAGGCTGTGGTTCGCAGTAAACGTGTAAAGCCTACCACAACTGATCGGCCTGATGAGATAGATGATACTCAGACAGAAGAGAAAACTGATACTGACAAGAACATGTCAACGATATTTGACATTCTAAGAAGGAAAAAGCGGGTTCGACTTGAAGCTTTGATATTAAACAGAAACTCTTTTGCGCAGACGGTGGAGAACTTATTTGCTCTGTCGTTCTTAGTCAAAGATGGCCGGGCTGAATTATCTGTGGATGCAAATGGTTTTCATACTGTTTCACCTAGGAATGCTCCTTCTGCTGAGGATGTGGCATCACGGAGGGTGACTTACCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGGTAATGATGGATAGTTTGCCTGTTGGTGAGGAGCTGATGCCTCATAGATCACCCCCAAACACCACACCTGCCTCTCAAGAGGAACAAGCAGCATACGATTCCCAAACAGCCTTACCCACAACCCCGATTCGAAAACTCTCCAGAAACCGTGGTCGGGTTGTGCAGGAAGAATCTATTGTAGAAGAATCCCCTGAAAATGATGATGCTACTGGATCTAACGTCATTCGCAGGTCTAAGCGCAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

390

Amino Acids

43.85

Weight (kDa)

8.31

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 997
Acc16I TGCGCA 1 cut(s) 738
Acc36I ACCTGC 2 cut(s) 997, 1140
AccII CGCG 4 cut(s) 21, 32, 62, 84
AciI CCGC 4 cut(s) 60, 154, 157, 694
AclWI GGATC 2 cut(s) 35, 1141
AcoI YGGCCR 1 cut(s) 784
AcyI GRCGYC 1 cut(s) 144
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 5 cut(s) 153, 232, 375, 434, 1075
AflIII ACRYGT 2 cut(s) 577, 657
AgsI TTSAA 4 cut(s) 496, 547, 707, 910
AhdI GACNNNNNGTC 1 cut(s) 121
AluBI AGCT 4 cut(s) 67, 710, 955, 1165
AluI AGCT 4 cut(s) 67, 710, 955, 1165
Alw26I GTCTC 2 cut(s) 129, 454
AlwI GGATC 2 cut(s) 35, 1141
Ama87I CYCGRG 2 cut(s) 42, 130
AoxI GGCC 2 cut(s) 602, 784
ApeKI GCWGC 2 cut(s) 64, 1010
AspA2I CCTAGG 1 cut(s) 833
AspLEI GCGC 6 cut(s) 21, 64, 84, 163, 739, 1161
AspS9I GGNCC 3 cut(s) 168, 348, 527
AsuC2I CCSGG 3 cut(s) 227, 376, 788
AsuHPI GGTGA 4 cut(s) 822, 886, 959, 963
AsuII TTCGAA 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 42, 130
AvaII GGWCC 3 cut(s) 168, 348, 527
AvrII CCTAGG 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 227
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 2 cut(s) 76, 1022
BccI CCATC 2 cut(s) 776, 922
BceAI ACGGC 3 cut(s) 59, 131, 167
BcnI CCSGG 3 cut(s) 227, 376, 788
BcoDI GTCTC 2 cut(s) 129, 454
BfaI CTAG 1 cut(s) 834
BfoI RGCGCY 1 cut(s) 164
BfuAI ACCTGC 2 cut(s) 997, 1140
BisI GCNGC 4 cut(s) 60, 65, 155, 1011
BlnI CCTAGG 1 cut(s) 833
BlsI GCNGC 4 cut(s) 61, 66, 156, 1012
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 3 cut(s) 227, 376, 788
Bme18I GGWCC 3 cut(s) 168, 348, 527
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 2 cut(s) 42, 130
BmgT120I GGNCC 3 cut(s) 168, 348, 527
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 3 cut(s) 227, 376, 788
BmsI GCATC 6 cut(s) 46, 301, 796, 872, 948, 1114
BpmI CTGGAG 1 cut(s) 1045
Bpu10I CCTNAGC 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 981
BpuMI CCSGG 3 cut(s) 227, 376, 788
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 43, 293, 833, 1069
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 232, 375, 434, 1075
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 369, 920, 1135
BseDI CCNNGG 4 cut(s) 43, 293, 833, 1069
BseGI GGATG 4 cut(s) 118, 445, 811, 862
BseLI CCNNNNNNNGG 5 cut(s) 153, 232, 375, 434, 1075
BseMII CTCAG 5 cut(s) 82, 465, 531, 638, 843
BseNI ACTGG 3 cut(s) 369, 920, 1135
BseRI GAGGAG 2 cut(s) 447, 965
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 2 cut(s) 76, 1022
BsgI GTGCAG 1 cut(s) 1103
Bsh1236I CGCG 4 cut(s) 21, 32, 62, 84
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 604, 786
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 2 cut(s) 42, 130
BsiSI CCGG 5 cut(s) 99, 166, 227, 375, 787
BslI CCNNNNNNNGG 5 cut(s) 153, 232, 375, 434, 1075
BsmAI GTCTC 2 cut(s) 129, 454
BsmBI CGTCTC 1 cut(s) 129
BsmI GAATGC 2 cut(s) 505, 844
BsnI GGCC 2 cut(s) 604, 786
BsoBI CYCGRG 2 cut(s) 42, 130
Bsp119I TTCGAA 1 cut(s) 1051
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 4 cut(s) 27, 598, 968, 1133
Bsp68I TCGCGA 1 cut(s) 32
BspACI CCGC 4 cut(s) 60, 154, 157, 694
BspANI GGCC 2 cut(s) 604, 786
BspCNI CTCAG 5 cut(s) 81, 464, 532, 637, 844
BspFNI CGCG 4 cut(s) 21, 32, 62, 84
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 2 cut(s) 997, 1140
BspPI GGATC 2 cut(s) 35, 1141
BspT104I TTCGAA 1 cut(s) 1051
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 3 cut(s) 369, 920, 1135
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 43, 293, 833, 1069
BssMI GATC 4 cut(s) 27, 598, 968, 1133
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 2 cut(s) 293, 833
Bst4CI ACNGT 4 cut(s) 366, 745, 826, 1070
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 3 cut(s) 100, 159, 1163
BstDEI CTNAG 8 cut(s) 68, 451, 540, 624, 680, 772, 852, 1155
BstDSI CCRYGG 1 cut(s) 1069
BstF5I GGATG 4 cut(s) 118, 445, 811, 862
BstFNI CGCG 4 cut(s) 21, 32, 62, 84
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 6 cut(s) 21, 64, 84, 163, 739, 1161
BstKTI GATC 4 cut(s) 30, 601, 971, 1136
BstMAI GTCTC 2 cut(s) 129, 454
BstMBI GATC 4 cut(s) 27, 598, 968, 1133
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 160, 848
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 3 cut(s) 225, 374, 786
BstSLI GKGCMC 1 cut(s) 227
BstUI CGCG 4 cut(s) 21, 32, 62, 84
BstV1I GCAGC 2 cut(s) 76, 1022
BstX2I RGATCY 1 cut(s) 1133
BstYI RGATCY 1 cut(s) 1133
BsuRI GGCC 2 cut(s) 604, 786
BtgI CCRYGG 1 cut(s) 1069
BtsCI GGATG 4 cut(s) 118, 445, 811, 862
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 3 cut(s) 205, 320, 376
BtuMI TCGCGA 1 cut(s) 32
BveI ACCTGC 2 cut(s) 997, 1140
Cac8I GCNNGC 3 cut(s) 100, 159, 1163
CfoI GCGC 6 cut(s) 21, 64, 84, 163, 739, 1161
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 168, 348, 527
CpoI CGGWCCG 1 cut(s) 168
CseI GACGC 1 cut(s) 152
Csp6I GTAC 1 cut(s) 537
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 5 cut(s) 275, 446, 506, 532, 658
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 8 cut(s) 68, 451, 540, 624, 680, 772, 852, 1155
DpnI GATC 4 cut(s) 29, 600, 970, 1135
DpnII GATC 4 cut(s) 27, 598, 968, 1133
DraIII CACNNNGTG 1 cut(s) 345
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 627
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 2 cut(s) 293, 833
Eco47I GGWCC 3 cut(s) 168, 348, 527
Eco88I CYCGRG 2 cut(s) 42, 130
EcoT14I CCWWGG 2 cut(s) 293, 833
ErhI CCWWGG 2 cut(s) 293, 833
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 5 cut(s) 278, 449, 509, 535, 661
FatI CATG 5 cut(s) 274, 445, 505, 531, 657
FauI CCCGC 2 cut(s) 150, 687
Fnu4HI GCNGC 4 cut(s) 60, 65, 155, 1011
FokI GGATG 4 cut(s) 125, 452, 818, 869
Fsp4HI GCNGC 4 cut(s) 60, 65, 155, 1011
FspBI CTAG 1 cut(s) 834
FspI TGCGCA 1 cut(s) 738
GlaI GCGC 6 cut(s) 20, 63, 83, 162, 738, 1160
GluI GCNGC 4 cut(s) 60, 65, 155, 1011
GsuI CTGGAG 1 cut(s) 1045
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 604, 786
HapII CCGG 5 cut(s) 99, 166, 227, 375, 787
HgaI GACGC 1 cut(s) 152
HhaI GCGC 6 cut(s) 21, 64, 84, 163, 739, 1161
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 5 cut(s) 278, 449, 509, 535, 661
Hin6I GCGC 6 cut(s) 19, 62, 82, 161, 737, 1159
HinP1I GCGC 6 cut(s) 19, 62, 82, 161, 737, 1159
HincII GTYRAC 3 cut(s) 52, 94, 663
HindII GTYRAC 3 cut(s) 52, 94, 663
HindIII AAGCTT 2 cut(s) 708, 1163
HinfI GANTC 7 cut(s) 34, 119, 230, 1019, 1048, 1091, 1106
HpaII CCGG 5 cut(s) 99, 166, 227, 375, 787
HphI GGTGA 4 cut(s) 822, 886, 959, 963
Hpy166II GTNNAC 5 cut(s) 52, 94, 348, 575, 663
Hpy188I TCNGA 8 cut(s) 71, 172, 235, 245, 288, 391, 627, 899
Hpy188III TCNNGA 6 cut(s) 31, 187, 265, 496, 998, 1062
Hpy8I GTNNAC 5 cut(s) 52, 94, 348, 575, 663
HpyAV CCTTC 6 cut(s) 82, 457, 550, 678, 855, 913
HpyCH4III ACNGT 4 cut(s) 366, 745, 826, 1070
HpyCH4IV ACGT 2 cut(s) 577, 1140
HpyCH4V TGCA 5 cut(s) 274, 314, 516, 809, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 848
HpyF3I CTNAG 8 cut(s) 68, 451, 540, 624, 680, 772, 852, 1155
HpySE526I ACGT 2 cut(s) 577, 1140
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 5 cut(s) 278, 449, 509, 535, 661
HspAI GCGC 6 cut(s) 19, 62, 82, 161, 737, 1159
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 27, 598, 968, 1133
LmnI GCTCC 3 cut(s) 168, 847, 952
Lsp1109I GCAGC 2 cut(s) 76, 1022
LweI GCATC 6 cut(s) 46, 301, 796, 872, 948, 1114
MaeI CTAG 1 cut(s) 834
MaeII ACGT 2 cut(s) 577, 1140
MaeIII GTNAC 3 cut(s) 360, 397, 874
MalI GATC 4 cut(s) 29, 600, 970, 1135
MboI GATC 4 cut(s) 27, 598, 968, 1133
MboII GAAGA 3 cut(s) 644, 1100, 1115
MflI RGATCY 1 cut(s) 1133
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 5 cut(s) 269, 415, 457, 468, 794
MlyI GAGTC 2 cut(s) 43, 128
MmeI TCCRAC 2 cut(s) 195, 414
MreI CGCCGGCG 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 98
MseI TTAA 5 cut(s) 357, 411, 456, 719, 1168
MspI CCGG 5 cut(s) 99, 166, 227, 375, 787
MspR9I CCNGG 3 cut(s) 227, 376, 788
Mva1269I GAATGC 2 cut(s) 505, 844
MvnI CGCG 4 cut(s) 21, 32, 62, 84
MwoI GCNNNNNNNGC 2 cut(s) 160, 848
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 3 cut(s) 227, 376, 788
NdeII GATC 4 cut(s) 27, 598, 968, 1133
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 5 cut(s) 278, 449, 509, 535, 661
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 2 cut(s) 360, 874
NruI TCGCGA 1 cut(s) 32
NsbI TGCGCA 1 cut(s) 738
NspI RCATGY 1 cut(s) 661
NspV TTCGAA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 997
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 574
PctI GAATGC 2 cut(s) 505, 844
PdiI GCCGGC 1 cut(s) 100
PfeI GAWTC 5 cut(s) 230, 1019, 1048, 1091, 1106
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 4 cut(s) 61, 66, 156, 1012
PleI GAGTC 2 cut(s) 42, 127
PpsI GAGTC 2 cut(s) 42, 127
PscI ACATGT 1 cut(s) 657
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 3 cut(s) 168, 348, 527
PsuI RGATCY 1 cut(s) 1133
PsyI GACNNNGTC 1 cut(s) 446
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SaqAI TTAA 5 cut(s) 357, 411, 456, 719, 1168
SatI GCNGC 4 cut(s) 60, 65, 155, 1011
Sau3AI GATC 4 cut(s) 27, 598, 968, 1133
Sau96I GGNCC 3 cut(s) 168, 348, 527
ScaI AGTACT 1 cut(s) 538
SchI GAGTC 2 cut(s) 43, 128
ScrFI CCNGG 3 cut(s) 227, 376, 788
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 6 cut(s) 46, 301, 796, 872, 948, 1114
SfuI TTCGAA 1 cut(s) 1051
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 3 cut(s) 168, 348, 527
SmlI CTYRAG 1 cut(s) 996
SmoI CTYRAG 1 cut(s) 996
Sse9I AATT 5 cut(s) 269, 415, 457, 468, 794
SsiI CCGC 4 cut(s) 60, 154, 157, 694
SspMI CTAG 1 cut(s) 834
StyD4I CCNGG 3 cut(s) 225, 374, 786
StyI CCWWGG 2 cut(s) 293, 833
TaaI ACNGT 4 cut(s) 366, 745, 826, 1070
TaiI ACGT 2 cut(s) 580, 1143
TaqI TCGA 3 cut(s) 264, 700, 1051
TaqII GACCGA 1 cut(s) 120
TasI AATT 5 cut(s) 269, 415, 457, 468, 794
TatI WGTACW 1 cut(s) 536
TauI GCSGC 2 cut(s) 62, 157
TfiI GAWTC 5 cut(s) 230, 1019, 1048, 1091, 1106
Tru1I TTAA 5 cut(s) 357, 411, 456, 719, 1168
Tru9I TTAA 5 cut(s) 357, 411, 456, 719, 1168
TscAI CASTG 3 cut(s) 205, 327, 376
TseFI GTSAC 2 cut(s) 360, 874
TseI GCWGC 2 cut(s) 64, 1010
Tsp45I GTSAC 2 cut(s) 360, 874
TspDTI ATGAA 2 cut(s) 273, 809
TspGWI ACGGA 2 cut(s) 40, 883
TspRI CASTG 3 cut(s) 205, 327, 376
Tth111I GACNNNGTC 1 cut(s) 446
VpaK11BI GGWCC 3 cut(s) 168, 348, 527
XceI RCATGY 1 cut(s) 661
XmaJI CCTAGG 1 cut(s) 833
XspI CTAG 1 cut(s) 834
ZrmI AGTACT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.