Rh5DG554900

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000940
Physical Location & Seq
Forward (+)
85689834 .. 85690526
693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG554900.1

Sequence Viewer

Length: 393 bp
ATGGCTTATTCAATAATCTCTTGTTCAGCTCCTACGAATGGTCCTGTTGCCAATCAAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGATGGAAATGGTGGCTGTTGGTGAGGAGCTGATGCCTCATAGAGACAGCACAAAGTGCACACCTGCCTCTAAGGAGCAACCAGCAGCATTCAATTCCCAAACAGCAACAGCCTTGCACACAACCCGGATTCAAAAACTTACCAGAAACCATGGCCTGGTTGTGCAGGAAAAATCTACTGCAGAAGAATTCTTTGAAAATGATGATGCTGTTGGAGCCACAGAACTCCGCAGGTGTAAGCGCAGGCTTTCGTTTAATACATGTTTAAGCGGAAGCTTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

130

Amino Acids

14.59

Weight (kDa)

7.73

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 181, 330
Acc36I ACCTGC 2 cut(s) 181, 330
AccB7I CCANNNNNTGG 1 cut(s) 265
AciI CCGC 2 cut(s) 337, 378
AcsI RAATTY 1 cut(s) 296
AdeI CACNNNGTG 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 38, 265
AflII CTTAAG 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 368
AgsI TTSAA 5 cut(s) 12, 94, 202, 242, 305
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 3 cut(s) 29, 139, 384
AluI AGCT 3 cut(s) 29, 139, 384
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 166
AoxI GGCC 1 cut(s) 262
ApaLI GTGCAC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 59, 143
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 206
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 147
BfmI CTRYAG 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 385
BfuAI ACCTGC 2 cut(s) 181, 330
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 235, 266
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 2 cut(s) 235, 266
BmsI GCATC 2 cut(s) 132, 304
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 265
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 38, 265
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 293
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 1 cut(s) 235
BslI CCNNNNNNNGG 2 cut(s) 38, 265
BsmAI GTCTC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 170
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 2 cut(s) 337, 378
BspANI GGCC 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 325
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 2 cut(s) 181, 330
BspTI CTTAAG 1 cut(s) 385
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 259
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 385
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 259
BstHHI GCGC 1 cut(s) 351
BstMAI GTCTC 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 165, 323
BstNI CCWGG 1 cut(s) 266
BstNSI RCATGY 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 233, 264
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 259
BveI ACCTGC 2 cut(s) 181, 330
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 2 cut(s) 260, 369
DdeI CTNAG 1 cut(s) 180
DraIII CACNNNGTG 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 263, 372
FaiI YATR 3 cut(s) 150, 261, 370
FatI CATG 2 cut(s) 259, 368
Fnu4HI GCNGC 1 cut(s) 195
Fsp4HI GCNGC 1 cut(s) 195
GlaI GCGC 1 cut(s) 350
GluI GCNGC 1 cut(s) 195
HaeIII GGCC 1 cut(s) 264
HapII CCGG 1 cut(s) 235
HhaI GCGC 1 cut(s) 351
Hin1II CATG 2 cut(s) 263, 372
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 1 cut(s) 238
HpaII CCGG 1 cut(s) 235
HphI GGTGA 2 cut(s) 59, 143
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 1 cut(s) 83
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4V TGCA 4 cut(s) 168, 226, 274, 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 323
HpyF3I CTNAG 1 cut(s) 180
Hsp92II CATG 2 cut(s) 263, 372
HspAI GCGC 1 cut(s) 349
LmnI GCTCC 4 cut(s) 34, 136, 184, 323
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 2 cut(s) 132, 304
MboII GAAGA 2 cut(s) 115, 305
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 2 cut(s) 202, 296
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 3 cut(s) 127, 156, 187
MseI TTAA 3 cut(s) 363, 374, 386
MspCI CTTAAG 1 cut(s) 385
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 2 cut(s) 235, 266
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 165, 323
NciI CCSGG 1 cut(s) 235
NcoI CCATGG 1 cut(s) 259
NlaIII CATG 2 cut(s) 263, 372
NlaIV GGNNCC 1 cut(s) 325
NspI RCATGY 1 cut(s) 372
PaqCI CACCTGC 2 cut(s) 181, 330
PciI ACATGT 1 cut(s) 368
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 1 cut(s) 196
PscI ACATGT 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 1 cut(s) 41
PstI CTGCAG 1 cut(s) 292
SaqAI TTAA 3 cut(s) 363, 374, 386
SatI GCNGC 1 cut(s) 195
Sau96I GGNCC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 235, 266
SduI GDGCHC 1 cut(s) 170
SetI ASST 5 cut(s) 31, 141, 175, 344, 386
SfaNI GCATC 2 cut(s) 132, 304
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 2 cut(s) 202, 296
SsiI CCGC 2 cut(s) 337, 378
StyD4I CCNGG 2 cut(s) 233, 264
StyI CCWWGG 1 cut(s) 259
TasI AATT 2 cut(s) 202, 296
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 3 cut(s) 363, 374, 386
Tru9I TTAA 3 cut(s) 363, 374, 386
TseI GCWGC 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 265
Vha464I CTTAAG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.